ndufaf2 cDNA ORF clone, Ictalurus punctatus(channel catfish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ndufaf2
Entrez Gene ID	100529027
Full Name	NADH:ubiquinone oxidoreductase complex assembly factor 2
Synonyms	mimit
Gene Type	protein-coding
Organism	Ictalurus punctatus(channel catfish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001201297.1	NP_001188226.1	mimitin, mitochondrial

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.
Liu S, Zhang Y, Zhou Z, Waldbieser G, Sun F, Lu J, Zhang J, Jiang Y, Zhang H, Wang X, Rajendran KV, Khoo L, Kucuktas H, Peatman E, Liu Z
BMC genomics13595(2012 Nov)

Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).
Chen F, Lee Y, Jiang Y, Wang S, Peatman E, Abernathy J, Liu H, Liu S, Kucuktas H, Ke C, Liu Z
PloS one5(7)e11546(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ndufaf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ndufaf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OIc39304	NM_001201297.1 Latest version!	Ictalurus punctatus NADH:ubiquinone oxidoreductase complex assembly factor 2 (ndufaf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ndufaf2 ( NM_001201297.1 ) cDNA ORF clone, Ictalurus punctatus(channel catfish) -> NP_001188226.1 Ictalurus punctatus NADH:ubiquinone oxidoreductase complex assembly factor 2 (ndufaf2), mRNA.

Price & Availability↑

CloneID	OIc39304
Clone ID Related Accession (Same CDS sequence)	NM_001201297.1
Accession Version	NM_001201297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 486bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Ictalurus punctatus(channel catfish)
Product	mimitin, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GU589419.1. ##Evidence-Data-START## Transcript exon combination :: GU589419.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00116755, SAMN04372583 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGAGTCGTT TTGCTTCTCT TCTAAGGAGG ACTTTTGGTG TAATGAAAGA ACATGTCGGA 
ACAGATCATT TAGGAAATAA ATACTATTAC ATACCCGAAC AGAAGACATG GACAGGCCGT 
GCTGTTCGGG CTCGGCGGTT TGTGGAGGCA TCCAATCCCT CAGAGTTTGA GTACCTCGAA 
GGAAGCATTC CCTCTGAATG GGATGCTTGG ATTAGAGGAC GACGCAAGGA TCCTCCTACT 
GTCGAGGAGT TGCTAAAGAA TGAACGTTAC AGAGAACAAA TCAAAGTCAA AGCACAGGAG 
GCTGGAGAGA AGGACCGAGA ACTGCAAGCC AAAGAGCTCG CAGAGGGGCT AATAACACAG 
CCGATGCAGA CTCAGGTCAA AGGTCACGCT GCTGCCACAC AGTTCGGGCA GTCAGAAATC 
AGCGAGGAGC CTGTGAGCAC TGCGAACACG TTCCAGCCCG GATCCTGGGC TCCTCCAAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001188226.1
CDS	55..540
Translation	MSRFASLLRRTFGVMKEHVGTDHLGNKYYYIPEQKTWTGRAVRARRFVEASNPSEFEYLEGSIPSEWDAWIRGRRKDPPTVEELLKNERYREQIKVKAQEAGEKDRELQAKELAEGLITQPMQTQVKGHAAATQFGQSEISEEPVSTANTFQPGSWAPPKK

Target ORF information:

RefSeq Version	NM_001201297.1
Organism	Ictalurus punctatus(channel catfish)
Definition	Ictalurus punctatus NADH:ubiquinone oxidoreductase complex assembly factor 2 (ndufaf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001201297.1

ORF Insert Sequence:

ATGAGTCGTT TTGCTTCTCT TCTAAGGAGG ACTTTTGGTG TAATGAAAGA ACATGTCGGA 
ACAGATCATT TAGGAAATAA ATACTATTAC ATACCCGAAC AGAAGACATG GACAGGCCGT 
GCTGTTCGGG CTCGGCGGTT TGTGGAGGCA TCCAATCCCT CAGAGTTTGA GTACCTCGAA 
GGAAGCATTC CCTCTGAATG GGATGCTTGG ATTAGAGGAC GACGCAAGGA TCCTCCTACT 
GTCGAGGAGT TGCTAAAGAA TGAACGTTAC AGAGAACAAA TCAAAGTCAA AGCACAGGAG 
GCTGGAGAGA AGGACCGAGA ACTGCAAGCC AAAGAGCTCG CAGAGGGGCT AATAACACAG 
CCGATGCAGA CTCAGGTCAA AGGTCACGCT GCTGCCACAC AGTTCGGGCA GTCAGAAATC 
AGCGAGGAGC CTGTGAGCAC TGCGAACACG TTCCAGCCCG GATCCTGGGC TCCTCCAAAA 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Efficient assembly and annotation of the transcriptome of catfish by RNA-Seq analysis of a doubled haploid homozygote.
BMC genomics13595(2012 Nov)
Liu S,Zhang Y,Zhou Z,Waldbieser G,Sun F,Lu J,Zhang J,Jiang Y,Zhang H,Wang X,Rajendran KV,Khoo L,Kucuktas H,Peatman E,Liu Z

Identification and characterization of full-length cDNAs in channel catfish (Ictalurus punctatus) and blue catfish (Ictalurus furcatus).
PloS one5(7)e11546(2010 Jul)
Chen F,Lee Y,Jiang Y,Wang S,Peatman E,Abernathy J,Liu H,Liu S,Kucuktas H,Ke C,Liu Z