CASP8AP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CASP8AP2
Entrez Gene ID	9994
Full Name	caspase 8 associated protein 2
Synonyms	CED-4,FLASH,RIP25
General protein information	Preferred Names caspase 8 associated protein 2 Names CASP8-associated protein 2 FLASH homolog RIP25 FLICE associated huge FLICE-associated huge protein human FLASH
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q15
Summary	This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008].
Disorder MIM:	606880

mRNA and Protein(s)

mRNA	Protein	Name
NM_001137668.1	NP_001131140.1	CASP8-associated protein 2
NM_012115.4	NP_036247.1	CASP8-associated protein 2
NM_001137667.1	NP_001131139.1	CASP8-associated protein 2
NM_012115.3	NP_036247.1	CASP8-associated protein 2
NM_001137668.2	NP_001131140.1	CASP8-associated protein 2
NM_001137667.2	NP_001131139.1	CASP8-associated protein 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CASP8AP2	XP_005210940.1
Pan troglodytes (chimpanzee)	CASP8AP2	XP_003311433.1
	CASP8AP2	XP_001098220.1
Rattus norvegicus (Norway rat)	Casp8ap2	NP_001101391.1
Mus musculus (house mouse)	Casp8ap2	NP_001116450.1
Homo sapiens (human)	CASP8AP2	NP_001131139.1
Canis lupus familiaris (dog)	CASP8AP2	XP_539042.3

Gene Ontology
Bibliography

Related articles in PubMed

U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.
Skrajna A, Yang XC, Bucholc K, Zhang J, Hall TMT, Dadlez M, Marzluff WF, Dominski Z
RNA (New York, N.Y.)23(6)938-951(2017 06)

The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3'-end processing.
Aik WS, Lin MH, Tan D, Tripathy A, Marzluff WF, Dominski Z, Chou CY, Tong L
PloS one12(10)e0186034(2017)

Regional Bias of Intratumoral Genetic Heterogeneity of Apoptosis-Related Genes BAX, APAF1, and FLASH in Colon Cancers with High Microsatellite Instability.
Choi MR, Gwak M, Yoo NJ, Lee SH
Digestive diseases and sciences60(6)1674-9(2015 Jun)

Low expressions of ARS2 and CASP8AP2 predict relapse and poor prognosis in pediatric acute lymphoblastic leukemia patients treated on China CCLG-ALL 2008 protocol.
Cui L, Gao C, Zhang RD, Jiao Y, Li WJ, Zhao XX, Liu SG, Yue ZX, Zheng HY, Deng GR, Wu MY, Li ZG, Jia HT
Leukemia research39(2)115-23(2015 Feb)

A conserved interaction that is essential for the biogenesis of histone locus bodies.
Yang XC, Sabath I, Kunduru L, van Wijnen AJ, Marzluff WF, Dominski Z
The Journal of biological chemistry289(49)33767-82(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

FLASH plays two roles in 3' end processing of histone pre-mRNAs: It interacts with Lsm11 to form a docking platform for the polyadenylation factors, and it cooperates with SLBP to recruit U7 snRNP to histone pre-mRNA.
Title: U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.

Our results indicate that the APAF1, BAX, and FLASH genes not only harbor frameshift mutations but also demonstrate mutational ITH, which together might play a role in the tumorigenesis of CRC with MSI-H by affecting the apoptosis of cancer cells.
Title: Regional Bias of Intratumoral Genetic Heterogeneity of Apoptosis-Related Genes BAX, APAF1, and FLASH in Colon Cancers with High Microsatellite Instability.

ARS2 and CASP8AP2 expressions can precisely predict high-risk of relapse and ALL prognosis.
Title: Low expressions of ARS2 and CASP8AP2 predict relapse and poor prognosis in pediatric acute lymphoblastic leukemia patients treated on China CCLG-ALL 2008 protocol.

The conserved C-terminal domain shared by FLASH, YARP, and Mute recognizes the C-terminal sequence of NPAT orthologues, thus acting as a signal targeting proteins to histone locus bodies.
Title: A conserved interaction that is essential for the biogenesis of histone locus bodies.

FLASH is required for embryogenesis, but not for cell proliferation or differentiation in embryonic stem cells.
Title: FLASH/casp8ap2 is indispensable for early embryogenesis but dispensable for proliferation and differentiation of ES cells.

The following CASP8AP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CASP8AP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06056	NM_001137668.2 Latest version!	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu06056	NM_001137667.2 Latest version!	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu06056	NM_012115.4 Latest version!	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
OHu06056	NM_012115.3	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu06056	NM_001137668.1	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu06056	NM_001137667.1	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CASP8AP2 ( NM_012115.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036247.1 Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 1, mRNA.

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CloneID	OHu06056
Clone ID Related Accession (Same CDS sequence)	NM_001137668.1 , NM_012115.4 , NM_001137667.1 , NM_012115.3 , NM_001137668.2 , NM_001137667.2
Accession Version	NM_012115.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	CASP8-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL353692.14, AF154415.1 and AF164678.2. On Aug 1, 2019 this sequence version replaced NM_012115.3. Transcript Variant: This variant (1) represents the longest transcript. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132830.1, AF154415.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036247.1
CDS	256..6204
Translation	MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR

Target ORF information:

RefSeq Version	NM_012115.4
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012115.4

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP8AP2 ( NM_001137668.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001131140.1 Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 3, mRNA.

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CloneID	OHu06056
Clone ID Related Accession (Same CDS sequence)	NM_001137668.1 , NM_012115.4 , NM_001137667.1 , NM_012115.3 , NM_001137668.2 , NM_001137667.2
Accession Version	NM_001137668.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	CASP8-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL353692.14, AF154415.1 and AF164678.2. On Aug 1, 2019 this sequence version replaced NM_001137668.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF164678.2 [ECO:0000331] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131140.1
CDS	84..6032
Translation	MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR

Target ORF information:

RefSeq Version	NM_001137668.2
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137668.2

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP8AP2 ( NM_001137667.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001131139.1 Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 2, mRNA.

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CloneID	OHu06056
Clone ID Related Accession (Same CDS sequence)	NM_001137668.1 , NM_012115.4 , NM_001137667.1 , NM_012115.3 , NM_001137668.2 , NM_001137667.2
Accession Version	NM_001137667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	CASP8-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL353692.14, BC132828.1 and AF164678.2. On Aug 1, 2019 this sequence version replaced NM_001137667.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC132828.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131139.1
CDS	217..6165
Translation	MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR

Target ORF information:

RefSeq Version	NM_001137667.2
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137667.2

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP8AP2 ( NM_001137668.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001131140.1 Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 3, mRNA.

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CloneID	OHu06056
Clone ID Related Accession (Same CDS sequence)	NM_001137668.1 , NM_012115.4 , NM_001137667.1 , NM_012115.3 , NM_001137668.2 , NM_001137667.2
Accession Version	NM_001137668.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	CASP8-associated protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP192859.1, BC132828.1 and AF154415.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.166674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131140.1
CDS	64..6012
Translation	MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR

Target ORF information:

RefSeq Version	NM_001137668.1
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137668.1

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP8AP2 ( NM_001137667.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001131139.1 Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 2, mRNA.

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CloneID	OHu06056
Clone ID Related Accession (Same CDS sequence)	NM_001137668.1 , NM_012115.4 , NM_001137667.1 , NM_012115.3 , NM_001137668.2 , NM_001137667.2
Accession Version	NM_001137667.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-08-10
Organism	Homo sapiens(human)
Product	CASP8-associated protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363682.1, BC132828.1 and AF154415.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.162252.1, SRR1803617.148363.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001131139.1
CDS	197..6145
Translation	MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR

Target ORF information:

RefSeq Version	NM_001137667.1
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001137667.1

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CASP8AP2 ( NM_012115.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036247.1 Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 1, mRNA.

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CloneID	OHu06056
Clone ID Related Accession (Same CDS sequence)	NM_001137668.1 , NM_012115.4 , NM_001137667.1 , NM_012115.3 , NM_001137668.2 , NM_001137667.2
Accession Version	NM_012115.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	CASP8-associated protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP363682.1, BC132830.1 and AF154415.1. On Nov 13, 2008 this sequence version replaced NM_012115.2. Transcript Variant: This variant (1) represents the longest transcript. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF154415.1, BC132830.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036247.1
CDS	236..6184
Translation	MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR

Target ORF information:

RefSeq Version	NM_012115.3
Organism	Homo sapiens(human)
Definition	Homo sapiens caspase 8 associated protein 2 (CASP8AP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012115.3

ORF Insert Sequence:

ATGGCAGCAG ATGATGACAA TGGTGATGGA ACAAGTTTAT TTGATGTCTT TTCTGCTTCT 
CCTCTTAAGA ACAATGATGA AGGCTCACTG GACATATACG CTGGGTTGGA CAGTGCTGTT 
TCTGACAGCG CTTCCAAATC CTGTGTACCA TCAAGAAATT GTTTGGACTT ATATGAAGAG 
ATCCTGACTG AAGAAGGAAC TGCAAAGGAG GCAACATATA ATGATTTGCA AGTAGAATAT 
GGAAAATGTC AACTACAAAT GAAAGAGCTG ATGAAAAAAT TTAAAGAAAT ACAGACACAG 
AATTTCAGCT TAATAAACGA AAACCAGTCT CTTAAGAAGA ATATTTCAGC ACTTATCAAA 
ACTGCCAGAG TGGAAATAAA CCGCAAGGAT GAAGAAATAA GTAATCTTCA CCAAAGATTG 
TCTGAGTTTC CACATTTTCG AAATAATCAT AAAACTGCAA GGACATTTGA TACAGTTAAA 
ACAAAAGATC TTAAATCTAG ATCTCCACAT TTGGATGATT GTTCAAAGAC TGATCACAGA 
GCTAAAAGTG ATGTTTCTAA AGATGTACAT CATAGCACTT CACTGCCAAA TCTGGAAAAG 
GAAGGAAAAC CACATTCTGA TAAAAGGAGT ACTTCACATT TACCTACATC TGTTGAGAAA 
CACTGCACTA ATGGTGTTTG GTCACGTTCT CATTATCAGG TTGGCGAGGG TAGCTCAAAT 
GAGGATAGTA GAAGAGGAAG AAAAGATATT AGACATAGCC AGTTTAACAG AGGAACTGAA 
AGAGTACGAA AAGACTTAAG TACTGGCTGT GGTGATGGTG AACCAAGGAT ATTGGAGGCT 
AGTCAAAGGC TACAAGGACA TCCTGAGAAA TATGGTAAAG GTGAACCAAA GACTGAAAGC 
AAAAGTTCGA AGTTTAAAAG TAACTCAGAT TCTGACTATA AAGGTGAACG CATTAACTCT 
TCTTGGGAGA AAGAGACCCC TGGAGAAAGG TCACACAGTC GAGTAGACTC TCAAAGTGAC 
AAAAAACTAG AAAGACAAAG TGAAAGATCA CAAAATATAA ATAGGAAAGA AGTTAAATCA 
CAAGACAAAG AAGAAAGAAA AGTTGATCAA AAACCTAAAT CAGTAGTAAA GGACCAAGAT 
CACTGGAGAA GATCTGAACG AGCATCACTT CCTCATTCCA AGAATGAAAT AACATTTTCT 
CATAATTCAA GTAAATACCA TCTAGAAGAG AGAAGAGGAT GGGAAGATTG TAAAAGAGAC 
AAGAGTGTAA ACAGTCATAG TTTTCAAGAT GGAAGATGTC CATCTTCTCT TTCAAACAGT 
AGAACTCACA AAAACATTGA CTCTAAGGAA GTTGATGCCA TGCATCAGTG GGAAAATACA 
CCTTTAAAAG CAGAAAGACA TAGAACTGAA GATAAGAGGA AAAGAGAACA AGAAAGCAAA 
GAAGAAAATA GGCATATTAG AAATGAAAAA AGAGTACCTA CAGAACATTT GCAGAAGACT 
AATAAGGAAA CTAAGAAAAC CACTACTGAT TTAAAGAAAC AGAATGAACC AAAGACTGAT 
AAGGGAGAAG TCCTTGATAA TGGTGTTTCT GAAGGAGCAG ATAATAAAGA GCTTGCAATG 
AAAGCTGAGA GTGGTCCAAA TGAAACAAAA AACAAGGACC TAAAATTGAG TTTTATGAAA 
AAATTGAACT TAACTCTTTC TCCTGCTAAA AAGCAACCTG TTTCCCAGGA TAATCAGCAT 
AAAATAACTG ATATTCCCAA GTCCAGTGGT GTATGTGATT CAGAGTCTTC AATGCAAGTT 
AAAACAGTGG CATATGTTCC CTCCATAAGT GAACATATCT TGGGGGAAGC AGCTGTCAGT 
GAACATACCA TGGGGGAAAC CAAGTCAACG TTATTGGAAC CAAAGGTTGC TCTTCTAGCA 
GTGACTGAAC CCAGGATCGG TATCTCAGAA ACCAACAAGG AAGACGAAAA TAGTTTGTTA 
GTTAGGTCTG TTGACAATAC TATGCATTGT GAAGAGCCCA TTTGTGGTAC AGAGACTTCC 
TTCCCATCTC CTATGGAAAT ACAACAGACA GAATCCTTGT TTCCATCAAC AGGAATGAAA 
CAAACCATTA ATAATGGAAG GGCAGCAGCT CCTGTGGTAA TGGATGTATT ACAAACAGAT 
GTGTCTCAAA ACTTTGGCTT GGAATTGGAT ACCAAAAGAA ATGATAATTC AGATTATTGT 
GGTATTTCTG AAGGTATGGA GATGAAGGTG GCACTTTCAA CAACAGTGAG TGAAACCACT 
GAAAGCATTT TGCAGCCTTC AATTGAGGAA GCTGATATTT TGCCAATAAT GCTTTCAGAA 
GATAATAACC CAAAATTTGA GCCTTCTGTT ATAGTTACAC CACTGGTTGA GAGTAAGTCG 
TGTCATCTGG AGCCTTGCTT ACCTAAAGAG ACTCTAGATT CTTCACTTCA GCAGACTGAG 
TTAATGGACC ACAGAATGGC AACTGGTGAA ACAAACTCAG TATATCATGA TGATGATAAC 
TCGGTTTTGA GCATTGACCT TAATCACCTG AGACCTATTC CAGAAGCCAT CAGTCCTCTG 
AATAGTCCAG TGAGACCTGT AGCAAAAGTT CTTAGAAATG AAAGCCCACC TCAAGTTCCA 
GTGTATAATA ACAGTCATAA AGATGTGTTT TTACCAAATT CAGCTCATTC TACCTCTAAG 
AGTCAGTCTG ATCTCAATAA GGAAAATCAA AAGCCAATTT ACAAATCTGA CAAATGTACA 
GAAGCAGACA CATGTAAGAA TTCACCATTA GATGAATTAG AAGAAGGAGA AATTAGAAGT 
GATAGTGAAA CATCTAAACC ACAAGAAAGT TTTGAAAAAA ATTCCAAACG TAGAGTGTCA 
GCTGATGTGC GGAAGTCAAA GACTATCCCA CGACGTGGGA AAAGTACTGT GTGTTTAGAT 
AAAGACAGTA GGAAAACACA TGTAAGAATC CATCAGACCA ATAACAAATG GAATAAAAGA 
CCTGATAAAT CTAGCAGATC TTCAAAAACA GAGAAGAAAG ATAAAGTGAT GAGCACTTCC 
AGCTTGGAAA AAATAGTTCC AATTATTGCT GTACCCTCTT CTGAACAAGA GATCATGCAC 
ATGTTACGAA TGATAAGAAA ACATGTAAGA AAAAATTATA TGAAATTCAA GGCAAAATTT 
TCATTAATAC AATTTCACAG AATTATTGAG TCAGCAATTT TGAGTTTTAC ATCTTTAATT 
AAACATCTCA ACTTACACAA AATCTCTAAG TCAGTGACTA CCTTACAGAA GAATCTCTGT 
GATATTATAG AGTCTAAACT TAAGCAAGTT AAAAAGAATG GCATAGTTGA TCGTTTATTT 
GAACAGCAAC TACCAGATAT GAAAAAAAAA TTGTGGAAGT TTGTAGATGA CCAACTTGAT 
TATTTGTTTG CAAAGCTTAA GAAAATCTTA GTATGTGATT CCAAAAGCTT TGGAAGAGAT 
AGTGATGAAG GCAAACTTGA AAAAACAAGT AAACAGAATG CACAGTATTC AAATAGTCAG 
AAAAGGAGTG TGGACAACTC CAACAGAGAA TTGCTGAAAG AAAAATTATC AAAATCAGAA 
GACCCTGTTC ATTATAAGTC TTTAGTGGGA TGTAAAAAAT CTGAGGAAAA TTATCAAGAC 
CAAAATAACT CCAGTATTAA CACTGTAAAG CATGACATTA AAAAAAATTT TAACATCTGC 
TTTGATAATA TAAAGAACTC TCAATCCGAA GAGCGCTCCT TGGAAGTACA CTGTCCAAGC 
ACCCCAAAGT CAGAAAAAAA CGAAGGAAGC AGCATAGAGG ATGCACAGAC ATCCCAGCAT 
GCAACTTTGA AGCCAGAACG AAGTTTCGAG ATTCTTACCG AACAGCAAGC ATCGAGCCTT 
ACTTTTAATT TAGTGAGTGA TGCACAAATG GGTGAAATAT TTAAAAGTTT GTTGCAAGGT 
TCTGATCTTT TAGACAGCAG TGTTAACTGT ACTGAAAAAA GTGAGTGGGA GTTAAAGACT 
CCAGAGAAGC AGCTGCTAGA GACTCTTAAG TGCGAGTCTA TACCAGCTTG TACAACAGAA 
GAGCTAGTTT CAGGGGTGGC TTCTCCATGT CCTAAAATGA TTAGTGATGA TAATTGGTCA 
TTATTATCAT CTGAAAAAGG TCCATCTCTG TCTTCAGGGC TTTCATTGCC GGTTCATCCT 
GATGTGTTGG ATGAAAGTTG TATGTTTGAA GTGTCTACTA ACCTACCTTT AAGTAAAGAT 
AATGTGTGTA GTGTAGAAAA GAGCAAGCCC TGCGTTTCTT CCATACTTCT TGAAGATCTA 
GCAGTCTCTT TAACAGTACC ATCGCCTCTG AAGTCAGATG GTCATCTCAG TTTTTTAAAG 
CCTGATATGT CGTCCAGTTC AACTCCTGAA GAAGTCATTA GTGCTCATTT TAGTGAAGAT 
GCCTTACTTG AGGAAGAGGA TGCATCTGAG CAAGATATTC ATTTAGCTCT GGAGTCTGAT 
AATTCAAGCA GTAAATCAAG TTGTTCTTCT TCCTGGACAA GCCGATCTGT TGCTCCAGGC 
TTTCAGTACC ACCCTAATCT ACCTATGCAT GCCGTCATAA TGGAAAAGTC CAATGATCAT 
TTCATTGTGA AAATACGACG TGCAACACCA TCTACCTCTT CTGGCCTTAA ACAGAGTATG 
ATGCCTGATG AATTATTGAC ATCTTTGCCC AGACATGGAA AGGAAGCTGA TGAAGGACCA 
GAGAAAGAAT ATATTTCATG TCAGAACACA GTTTTTAAAT CTGTGGAGGA ATTGGAAAAC 
TCCAACAAAA ATGTTGATGG CAGCAAGTCA ACTCATGAAG AACAGAGCTC TATGATACAA 
ACACAGGTTC CTGATATATA TGAATTTCTT AAAGATGCTT CAGATAAGAT GGGTCATAGT 
GATGAAGTGG CTGATGAATG TTTCAAATTG CATCAAGTAT GGGAAACAAA AGTGCCTGAA 
AGCATTGAAG AATTGCCTTC AATGGAAGAA ATCTCACACT CTGTTGGGGA ACATCTTCCA 
AACACATACG TAGATCTAAC GAAAGATCCA GTCACTGAAA CCAAAAACTT GGGGGAATTC 
ATAGAAGTAA CAGTTTTACA TATTGATCAG TTGGGATGTT CTGGAGGCAA TTTAAATCAG 
AGTGCTCAAA TATTAGACAA TTCTTTGCAG GCTGATACTG TAGGTGCTTT TATTGATTTG 
ACACAAGATG CTTCAAGTGA GGCTAAAAGT GAAGGTAATC ATCCTGCATT AGCTGTGGAA 
GACTTGGGAT GTGGGGTGAT ACAGGTAGAT GAAGATAATT GTAAGGAAGA AAAGGCACAA 
GTGGCAAACA GGCCTTTAAA ATGCATTGTT GAGGAAACCT ATATCGACTT GACCACAGAA 
TCTCCCAGTT CATGTGAAGT AAAAAAAGAT GAGTTAAAAT CAGAGCCAGG ATCAAATTGT 
GATAACTCGG AGTTGCCTGG GACTTTGCAT AATTCTCACA AAAAGAGAAG AAACATTTCT 
GATCTAAATC ATCCTCATAA AAAACAAAGA AAGGAAACAG ACTTAACTAA TAAGGAAAAG 
ACCAAGAAAC CTACCCAAGA TTCTTGTGAG AATACTGAAG CTCACCAAAA GAAAGCCAGT 
AAGAAGAAGG CCCCTCCTGT GACTAAAGAT CCCTCATCAT TAAAGGCAAC CCCAGGGATT 
AAGGATTCAT CAGCAGCACT TGCCACTTCT ACAAGTCTTT CTGCAAAAAA TGTTATTAAA 
AAGAAGGGAG AAATTATCAT TTTATGGACA AGAAATGATG ACCGGGAAAT TTTATTGGAG 
TGTCAGAAAA GAGGGCCATC ATTTAAAACA TTTGCATATT TAGCCGCCAA GTTGGATAAA 
AATCCAAATC AGGTCTCAGA AAGATTCCAG CAGCTAATGA AGCTCTTTGA AAAGTCAAAA 
TGCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

U7 snRNP is recruited to histone pre-mRNA in a FLASH-dependent manner by two separate regions of the stem-loop binding protein.
RNA (New York, N.Y.)23(6)938-951(2017 06)
Skrajna A,Yang XC,Bucholc K,Zhang J,Hall TMT,Dadlez M,Marzluff WF,Dominski Z

The N-terminal domains of FLASH and Lsm11 form a 2:1 heterotrimer for histone pre-mRNA 3'-end processing.
PloS one12(10)e0186034(2017)
Aik WS,Lin MH,Tan D,Tripathy A,Marzluff WF,Dominski Z,Chou CY,Tong L

Regional Bias of Intratumoral Genetic Heterogeneity of Apoptosis-Related Genes BAX, APAF1, and FLASH in Colon Cancers with High Microsatellite Instability.
Digestive diseases and sciences60(6)1674-9(2015 Jun)
Choi MR,Gwak M,Yoo NJ,Lee SH

Low expressions of ARS2 and CASP8AP2 predict relapse and poor prognosis in pediatric acute lymphoblastic leukemia patients treated on China CCLG-ALL 2008 protocol.
Leukemia research39(2)115-23(2015 Feb)
Cui L,Gao C,Zhang RD,Jiao Y,Li WJ,Zhao XX,Liu SG,Yue ZX,Zheng HY,Deng GR,Wu MY,Li ZG,Jia HT

A conserved interaction that is essential for the biogenesis of histone locus bodies.
The Journal of biological chemistry289(49)33767-82(2014 Dec)
Yang XC,Sabath I,Kunduru L,van Wijnen AJ,Marzluff WF,Dominski Z

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CASP8AP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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