MED13 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED13
Entrez Gene ID	9969
Full Name	mediator complex subunit 13
Synonyms	ARC250,DRIP250,HSPC221,THRAP1,TRAP240
General protein information	Preferred Names mediator complex subunit 13 Names mediator of RNA polymerase II transcription subunit 13 activator-recruited cofactor 250 kDa component mediator of RNA polymerase II transcription, subunit 13 homolog thyroid hormone receptor-associated protein 1 thyroid hormone receptor-associated protein complex 240 kDa component thyroid hormone receptor-associated protein complex component TRAP240 thyroid hormone receptor-associated protein, 240 kDa subunit vitamin D3 receptor-interacting protein complex component DRIP250
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q23.2
Summary	This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of this gene is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. [provided by RefSeq, Jul 2008].
Disorder MIM:	603808

mRNA and Protein(s)

mRNA	Protein	Name
XM_011525553.3	XP_011523855.1	mediator of RNA polymerase II transcription subunit 13 isoform X3
XM_011525551.2	XP_011523853.1	mediator of RNA polymerase II transcription subunit 13 isoform X1
XM_011525552.2	XP_011523854.1	mediator of RNA polymerase II transcription subunit 13 isoform X2
NM_005121.2	NP_005112.2	mediator of RNA polymerase II transcription subunit 13
XM_011525553.2	XP_011523855.1	mediator of RNA polymerase II transcription subunit 13 isoform X3
NM_005121.3	NP_005112.2	mediator of RNA polymerase II transcription subunit 13

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	med13a	XP_005172748.1
Xenopus tropicalis (tropical clawed frog)	med13	XP_004911678.1
Homo sapiens (human)	MED13	NP_005112.2
Pan troglodytes (chimpanzee)	MED13	XP_001138050.1
Drosophila melanogaster (fruit fly)	skd	NP_001262134.1
Canis lupus familiaris (dog)	MED13	XP_005625048.1
Bos taurus (cattle)	MED13	XP_002695649.2
Mus musculus (house mouse)	Med13	NP_001074400.1
	MED13	XP_001110128.2
Rattus norvegicus (Norway rat)	Med13	NP_001100505.1
Gallus gallus (chicken)	MED13	XP_004946777.1

Gene Ontology
Bibliography

Related articles in PubMed

Frameshift Mutations of HSPA4 and MED13 in Gastric and Colorectal Cancers.
Jo YS, Choi MR, Song SY, Kim MS, Yoo NJ, Lee SH
Pathology oncology research : POR22(4)769-72(2016 Oct)

Mediator kinase module and human tumorigenesis.
Clark AD, Oldenbroek M, Boyer TG
Critical reviews in biochemistry and molecular biology50(5)393-426(2015)

MED13-dependent signaling from the heart confers leanness by enhancing metabolism in adipose tissue and liver.
Baskin KK, Grueter CE, Kusminski CM, Holland WL, Bookout AL, Satapati S, Kong YM, Burgess SC, Malloy CR, Scherer PE, Newgard CB, Bassel-Duby R, Olson EN
EMBO molecular medicine6(12)1610-21(2014 Dec)

Genetic determinants of P wave duration and PR segment.
Verweij N, Mateo Leach I, van den Boogaard M, van Veldhuisen DJ, Christoffels VM, , Hillege HL, van Gilst WH, Barnett P, de Boer RA, van der Harst P
Circulation. Cardiovascular genetics7(4)475-81(2014 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Each of HSPA4 and MED13 mutations were detected in GC.
Title: Frameshift Mutations of HSPA4 and MED13 in Gastric and Colorectal Cancers.

Data suggest that MED13, MED12, CDK8 and cyclin C (CycC) comprise a four-subunit "kinase" module of the Mediator complex that functions as a major ingress of oncogenic and developmental signaling/gene expression in humans. [REVIEW]
Title: Mediator kinase module and human tumorigenesis.

Cardiac overexpression of MED13 in transgenic mice confers a lean phenotype that is associated with increased lipid uptake, beta-oxidation and mitochondrial content in white adipose tissue and liver
Title: MED13-dependent signaling from the heart confers leanness by enhancing metabolism in adipose tissue and liver.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: The variant rs1867277 in FOXE1 gene confers thyroid cancer susceptibility through the recruitment of USF1/USF2 transcription factors.

The following MED13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu63562	XM_011525552.2 Latest version!	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu63563	XM_011525553.3 Latest version!	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu04704	NM_005121.3 Latest version!	Homo sapiens mediator complex subunit 13 (MED13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu63561	XM_011525551.2 Latest version!	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu63563	XM_011525553.2	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu04704	NM_005121.2	Homo sapiens mediator complex subunit 13 (MED13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED13 ( XM_011525552.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523854.1 Homo sapiens mediator complex subunit 13 (MED13), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu63562
Clone ID Related Accession (Same CDS sequence)	XM_011525552.2
Accession Version	XM_011525552.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 13 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525552.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTGGAG CTGTTTGCTG 
AGTCGTCGAA ACTTGCAGTC TCTAAGTAAA AGGCTCAAAG ACATGTGTAG AATGTGTGGT 
ATATCTGCTG CAGACTCCCC TAGCATTCTC AGTGCTTGCT TGGTGGCAAT GGAGCCGCAA 
GGCTCTTTTG TTATTATGCC AGATTCTGTG TCAACTGGTT CTGTATTTGG AAGAAGCACG 
ACTCTAAATA TGCAGACATC TCAGCTAAAT ACCCCACAGG ATACATCATG TACTCATATA 
CTTGTGTTTC CTACTTCTGC TTCTGTGCAA GTAGCTTCAG CTACTTATAC CACTGAAAAT 
TTGGATTTAG CTTTCAATCC CAACAATGGA CCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523854.1
CDS	779..6757
Translation	MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPSFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGCSMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHPDVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNGPL

Target ORF information:

RefSeq Version	XM_011525552.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525552.2

ORF Insert Sequence:

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTGGAG CTGTTTGCTG 
AGTCGTCGAA ACTTGCAGTC TCTAAGTAAA AGGCTCAAAG ACATGTGTAG AATGTGTGGT 
ATATCTGCTG CAGACTCCCC TAGCATTCTC AGTGCTTGCT TGGTGGCAAT GGAGCCGCAA 
GGCTCTTTTG TTATTATGCC AGATTCTGTG TCAACTGGTT CTGTATTTGG AAGAAGCACG 
ACTCTAAATA TGCAGACATC TCAGCTAAAT ACCCCACAGG ATACATCATG TACTCATATA 
CTTGTGTTTC CTACTTCTGC TTCTGTGCAA GTAGCTTCAG CTACTTATAC CACTGAAAAT 
TTGGATTTAG CTTTCAATCC CAACAATGGA CCCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED13 ( XM_011525553.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523855.1 Homo sapiens mediator complex subunit 13 (MED13), transcript variant X3, mRNA.

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CloneID	OHu63563
Clone ID Related Accession (Same CDS sequence)	XM_011525553.3 , XM_011525553.2
Accession Version	XM_011525553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 13 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGATT CAGCTACAAA AAAATTAATT GGTGAATGGA AACAGTTCTA TCCTATCTCA 
TGTTGCTTGA AGGAGATGTC TGAAGAAAAA CAGGAAGATA TGGATTGGGA AGATGATTCT 
TTAGCTGCAG TAGAAGTTCT TGTTGCTGGT GTCCGAATGA TCTACCCAGC ATGCTTTGTT 
CTAGTCCCTC AGTCAGACAT TCCTACTCCT AGCCCTGTGG GATCCACTCA CTGTTCATCT 
TCTTGCTTGG GTGTCCACCA AGTGCCTGCT TCCACAAGAG ATCCTGCTAT GTCTTCGGTT 
ACGCTTACAC CACCTACGTC TCCTGAGGAA GTCCAAACAG TTGATCCTCA GTCTGTCCAG 
AAGTGGGTCA AATTTTCTTC AGTATCTGAT GGCTTCAACT CCGATAGTAC TAGCCACCAT 
GGTGGGAAAA TACCCAGAAA ATTAGCAAAT CATGTGGTGG ATAGAGTTTG GCAAGAATGC 
AATATGAACA GAGCACAGAA CAAGAGGAAG TATTCTGCTT CATCAGGTGG TCTATGCGAA 
GAAGCGACAG CTGCTAAAGT GGCATCCTGG GATTTTGTTG AAGCCACACA AAGAACAAAT 
TGCAGTTGTT TGAGGCACAA AAATCTCAAG TCAAGAAATG CTGGACAACA AGGACAGGCA 
CCATCTTTAG GTCAGCAACA ACAAATACTT CCTAAGCACA AGACCAATGA GAAGCAAGAA 
AAGAGTGAAA AGCCACAGAA ACGCCCCTTG ACTCCTTTTC ACCATCGTGT GTCTGTTAGT 
GATGATGTTG GCATGGACGC AGATTCAGCC AGCCAAAGAC TTGTGATCTC TGCTCCAGAC 
AGTCAAGTGA GATTTTCAAA TATCCGAACT AATGATGTAG CAAAGACTCC TCAGATGCAT 
GGCACCGAAA TGGCAAATTC ACCTCAACCA CCCCCACTTA GTCCTCACCC TTGTGATGTG 
GTTGATGAAG GAGTGACTAA AACACCTTCA ACTCCTCAGA GTCAACATTT TTATCAAATG 
CCAACACCAG ATCCCTTGGT TCCTTCTAAA CCAATGGAAG ATAGGATAGA CAGTTTGTCC 
CAGTCTTTCC CACCTCAATA TCAGGAAGCT GTAGAACCTA CAGTATATGT TGGTACAGCA 
GTAAACTTGG AAGAAGATGA AGCCAATATA GCCTGGAAGT ATTACAAGTT CCCAAAGAAA 
AAAGATGTAG AGTTTTTACC ACCTCAACTT CCAAGTGATA AATTCAAGGA TGATCCAGTT 
GGACCTTTTG GACAGGAAAG TGTAACATCA GTTACAGAGT TAATGGTGCA ATGTAAGAAA 
CCTTTAAAAG TTTCTGATGA ATTAGTGCAG CAATATCAAA TTAAAAACCA GTGTCTTTCA 
GCAATAGCAT CTGATGCAGA ACAAGAACCT AAAATTGATC CATATGCATT TGTTGAAGGA 
GATGAGGAAT TCCTTTTTCC TGATAAAAAA GATAGACAAA ATAGTGAGAG AGAAGCTGGA 
AAAAAACACA AGGTAGAAGA TGGGACATCT AGTGTAACAG TGTTATCACA TGAAGAAGAT 
GCTATGTCAT TATTTAGTCC CTCTATCAAG CAAGATGCTC CACGCCCTAC TAGTCATGCC 
CGTCCTCCAT CAACAAGTTT GATTTATGAC TCAGACCTGG CTGTCTCTTA TACTGACCTT 
GATAATCTCT TCAATTCTGA TGAAGATGAA CTAACACCTG GATCTAAAAA ATCAGCAAAT 
GGATCAGATG ATAAAGCCAG CTGCAAGGAA TCAAAGACAG GAAATCTGGA CCCGTTATCT 
TGCATAAGCA CTGCAGATCT TCATAAAATG TATCCTACAC CACCATCATT GGAACAACAT 
ATTATGGGAT TTTCCCCAAT GAATATGAAT AATAAAGAAT ATGGTAGTAT GGATACAACA 
CCTGGAGGAA CTGTTCTAGA AGGAAATAGT TCTAGTATAG GAGCGCAGTT CAAAATTGAG 
GTTGATGAGG GATTCTGTAG CCCCAAACCT TCTGAAATTA AAGATTTTTC TTATGTCTAT 
AAGCCTGAAA ATTGTCAAAT TCTAGTGGGA TGTTCCATGT TTGCACCTCT AAAAACTCTA 
CCAAGCCAAT ATCTGCCCCC TATCAAATTG CCAGAAGAGT GTATTTACCG TCAGAGTTGG 
ACTGTTGGAA AATTGGAATT GCTTTCTTCA GGGCCTTCAA TGCCATTCAT CAAAGAGGGT 
GATGGAAGTA ATATGGATCA AGAATATGGC ACTGCTTATA CACCTCAAAC TCATACTTCT 
TTTGGGATGC CTCCTAGCAG TGCACCTCCT AGTAACAGCG GAGCAGGAAT TCTTCCTTCT 
CCATCCACCC CTCGGTTTCC AACTCCAAGG ACTCCAAGGA CTCCTCGGAC TCCTCGTGGA 
GCTGGTGGAC CTGCTAGTGC TCAAGGTTCA GTCAAATATG AAAATTCAGA CTTGTATTCA 
CCAGCTTCTA CCCCATCTAC ATGCAGACCC CTTAATTCTG TTGAACCTGC AACTGTCCCT 
TCCATCCCTG AAGCACACAG TCTTTATGTA AACCTCATCC TTTCAGAATC AGTTATGAAT 
TTGTTTAAAG ACTGTAACTT TGATAGTTGT TGCATCTGTG TTTGCAACAT GAACATCAAG 
GGTGCCGATG TTGGAGTTTA CATTCCAGAT CCAACGCAGG AAGCACAATA TAGGTGTACC 
TGTGGCTTCA GTGCTGTCAT GAACAGAAAA TTTGGAAACA ATTCAGGATT ATTTCTTGAA 
GATGAACTAG ATATCATAGG ACGCAATACA GACTGTGGCA AAGAAGCAGA AAAACGTTTT 
GAAGCTCTCA GGGCTACCTC TGCTGAACAT GTTAATGGAG GACTAAAGGA ATCTGAAAAA 
TTATCTGATG ATTTGATATT ATTGCTACAA GATCAGTGCA CTAATTTATT TTCACCCTTT 
GGAGCAGCAG ACCAAGATCC TTTTCCTAAA AGTGGTGTAA TTAGCAATTG GGTACGTGTT 
GAAGAGCGTG ACTGTTGCAA TGACTGCTAC CTTGCATTAG AACATGGGCG TCAGTTCATG 
GATAACATGT CAGGAGGAAA AGTTGATGAA GCACTTGTGA AAAGTTCATG CTTACACCCC 
TGGTCCAAAA GAAACGATGT GAGTATGCAG TGCTCACAGG ATATACTTCG AATGCTCCTC 
TCTCTTCAGC CAGTTCTTCA GGATGCCATT CAGAAAAAAA GAACAGTAAG ACCTTGGGGT 
GTTCAGGGTC CTCTCACTTG GCAACAATTT CATAAAATGG CTGGCCGAGG CTCTTATGGA 
ACTGATGAAT CCCCAGAACC ACTGCCAATC CCCACATTTT TGTTGGGTTA TGATTATGAT 
TATCTGGTGC TTTCTCCATT TGCTCTTCCT TATTGGGAGA GACTTATGCT GGAACCCTAT 
GGATCTCAAA GAGATATAGC CTATGTTGTA CTGTGTCCAG AAAATGAAGC CTTGTTAAAT 
GGAGCAAAAA GCTTTTTTAG AGATCTTACT GCAATATATG AGTCCTGTCG ATTAGGTCAA 
CATAGACCTG TTTCTCGACT GTTAACAGAT GGGATCATGA GAGTTGGATC TACTGCATCA 
AAGAAACTAT CAGAAAAGTT GGTAGCAGAA TGGTTTTCTC AGGCAGCTGA CGGTAACAAT 
GAAGCATTTT CTAAACTCAA GCTTTATGCA CAAGTCTGCA GATATGACCT AGGTCCTTAT 
CTTGCTTCCC TGCCATTGGA CAGCTCTCTA CTTTCCCAGC CAAATTTAGT TGCCCCTACA 
AGTCAGTCTT TGATTACTCC ACCTCAGATG ACAAATACTG GAAATGCTAA TACTCCATCT 
GCCACCTTAG CATCTGCAGC GAGCAGCACT ATGACAGTGA CTTCAGGTGT TGCCATATCT 
ACTTCAGTTG CCACAGCTAA TTCAACTTTG ACCACAGCTT CAACTTCATC TTCATCATCC 
TCCAACTTGA ATAGTGGAGT ATCATCAAAT AAACTACCTT CGTTTCCACC CTTTGGCAGT 
ATGAACAGTA ATGCTGCAGG ATCCATGTCT ACACAAGCAA ATACAGTTCA GAGTGGTCAG 
CTAGGAGGGC AACAGACATC AGCTCTACAG ACAGCTGGGA TTTCTGGAGA ATCATCTTCA 
CTTCCCACTC AGCCGCATCC TGATGTGTCT GAAAGCACGA TGGATCGGGA TAAAGTGGGA 
ATCCCCACAG ATGGTGATTC ACATGCAGTC ACGTATCCAC CTGCAATTGT TGTTTATATA 
ATTGATCCTT TTACATACGA AAATACAGAC GAGAGCACTA ACTCTTCTAG TGTGTGGACA 
TTGGGGCTAC TTCGATGCTT TCTAGAAATG GTCCAGACTC TTCCTCCTCA TATCAAGAGT 
ACTGTTTCTG TACAGATTAT TCCTTGTCAG TACCTGTTGC AACCTGTGAA GCATGAAGAT 
AGAGAAATCT ATCCCCAGCA TTTAAAATCC CTGGCTTTTT CGGCCTTTAC CCAGTGTCGG 
AGGCCACTTC CAACATCAAC CAATGTGAAA ACATTGACTG GCTTTGGTCC AGGTTTAGCC 
ATGGAAACTG CCCTTAGAAG TCCTGATAGA CCAGAGTGTA TTCGACTTTA TGCACCTCCT 
TTTATTCTGG CTCCAGTGAA GGACAAACAG ACAGAGCTAG GAGAAACATT TGGAGAAGCT 
GGACAGAAAT ATAATGTTCT TTTTGTGGGA TACTGTTTAT CACATGATCA AAGGTGGATT 
CTTGCATCTT GCACAGATCT ATATGGAGAA CTTTTAGAAA CTTGTATCAT TAACATCGAT 
GTTCCAAATA GGGCTCGTCG GAAAAAAAGT TCTGCTAGAA AATTTGGTCT ACAGAAACTT 
TGGGAGTGGT GCTTAGGACT TGTACAAATG AGTTCATTGC CATGGAGAGT TGTAATTGGT 
CGTCTAGGAA GGATTGGTCA TGGAGAATTG AAAGATTGGA GCTGTTTGCT GAGTCGTCGA 
AACTTGCAGT CTCTAAGTAA AAGGCTCAAA GACATGTGTA GAATGTGTGG TATATCTGCT 
GCAGACTCCC CTAGCATTCT CAGTGCTTGC TTGGTGGCAA TGGAGCCGCA AGGCTCTTTT 
GTTATTATGC CAGATTCTGT GTCAACTGGT TCTGTATTTG GAAGAAGCAC GACTCTAAAT 
ATGCAGACAT CTCAGCTAAA TACCCCACAG GATACATCAT GTACTCATAT ACTTGTGTTT 
CCTACTTCTG CTTCTGTGCA AGTAGCTTCA GCTACTTATA CCACTGAAAA TTTGGATTTA 
GCTTTCAATC CCAACAATGA TGGAGCAGAT GGAATGGGTA TCTTTGATTT GTTAGACACA 
GGAGATGATC TTGACCCTGA TATCATTAAT ATCCTTCCTG CTTCTCCAAC TGGTTCTCCT 
GTACATTCTC CAGGATCTCA TTACCCCCAT GGAGGTGATG CGGGCAAGGG TCAGAGTACT 
GATCGGCTAC TATCAACAGA ACCTCATGAG GAAGTACCTA ATATTCTTCA GCAACCATTG 
GCCCTTGGTT ACTTTGTATC AACTGCCAAA GCAGGTCCAT TACCTGACTG GTTCTGGTCA 
GCATGTCCTC AAGCACAATA TCAGTGTCCC CTTTTTCTTA AGGCCTCTTT GCACCTCCAC 
GTGCCTTCAG TGCAATCTGA CGAGCTGCTT CACAGTAAAC ACTCCCACCC ACTTGACTCA 
AATCAGACTT CAGATGTCCT CAGGTTTGTT TTGGAACAGT ACAATGCACT CTCCTGGCTA 
ACCTGTGACC CTGCAACCCA GGACAGACGC TCATGTCTCC CAATTCATTT TGTGGTGCTG 
AATCAGTTAT ATAACTTTAT TATGAATATG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523855.1
CDS	145..6000
Translation	MSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGCSMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHPDVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML

Target ORF information:

RefSeq Version	XM_011525553.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525553.3

ORF Insert Sequence:

ATGTCTGATT CAGCTACAAA AAAATTAATT GGTGAATGGA AACAGTTCTA TCCTATCTCA 
TGTTGCTTGA AGGAGATGTC TGAAGAAAAA CAGGAAGATA TGGATTGGGA AGATGATTCT 
TTAGCTGCAG TAGAAGTTCT TGTTGCTGGT GTCCGAATGA TCTACCCAGC ATGCTTTGTT 
CTAGTCCCTC AGTCAGACAT TCCTACTCCT AGCCCTGTGG GATCCACTCA CTGTTCATCT 
TCTTGCTTGG GTGTCCACCA AGTGCCTGCT TCCACAAGAG ATCCTGCTAT GTCTTCGGTT 
ACGCTTACAC CACCTACGTC TCCTGAGGAA GTCCAAACAG TTGATCCTCA GTCTGTCCAG 
AAGTGGGTCA AATTTTCTTC AGTATCTGAT GGCTTCAACT CCGATAGTAC TAGCCACCAT 
GGTGGGAAAA TACCCAGAAA ATTAGCAAAT CATGTGGTGG ATAGAGTTTG GCAAGAATGC 
AATATGAACA GAGCACAGAA CAAGAGGAAG TATTCTGCTT CATCAGGTGG TCTATGCGAA 
GAAGCGACAG CTGCTAAAGT GGCATCCTGG GATTTTGTTG AAGCCACACA AAGAACAAAT 
TGCAGTTGTT TGAGGCACAA AAATCTCAAG TCAAGAAATG CTGGACAACA AGGACAGGCA 
CCATCTTTAG GTCAGCAACA ACAAATACTT CCTAAGCACA AGACCAATGA GAAGCAAGAA 
AAGAGTGAAA AGCCACAGAA ACGCCCCTTG ACTCCTTTTC ACCATCGTGT GTCTGTTAGT 
GATGATGTTG GCATGGACGC AGATTCAGCC AGCCAAAGAC TTGTGATCTC TGCTCCAGAC 
AGTCAAGTGA GATTTTCAAA TATCCGAACT AATGATGTAG CAAAGACTCC TCAGATGCAT 
GGCACCGAAA TGGCAAATTC ACCTCAACCA CCCCCACTTA GTCCTCACCC TTGTGATGTG 
GTTGATGAAG GAGTGACTAA AACACCTTCA ACTCCTCAGA GTCAACATTT TTATCAAATG 
CCAACACCAG ATCCCTTGGT TCCTTCTAAA CCAATGGAAG ATAGGATAGA CAGTTTGTCC 
CAGTCTTTCC CACCTCAATA TCAGGAAGCT GTAGAACCTA CAGTATATGT TGGTACAGCA 
GTAAACTTGG AAGAAGATGA AGCCAATATA GCCTGGAAGT ATTACAAGTT CCCAAAGAAA 
AAAGATGTAG AGTTTTTACC ACCTCAACTT CCAAGTGATA AATTCAAGGA TGATCCAGTT 
GGACCTTTTG GACAGGAAAG TGTAACATCA GTTACAGAGT TAATGGTGCA ATGTAAGAAA 
CCTTTAAAAG TTTCTGATGA ATTAGTGCAG CAATATCAAA TTAAAAACCA GTGTCTTTCA 
GCAATAGCAT CTGATGCAGA ACAAGAACCT AAAATTGATC CATATGCATT TGTTGAAGGA 
GATGAGGAAT TCCTTTTTCC TGATAAAAAA GATAGACAAA ATAGTGAGAG AGAAGCTGGA 
AAAAAACACA AGGTAGAAGA TGGGACATCT AGTGTAACAG TGTTATCACA TGAAGAAGAT 
GCTATGTCAT TATTTAGTCC CTCTATCAAG CAAGATGCTC CACGCCCTAC TAGTCATGCC 
CGTCCTCCAT CAACAAGTTT GATTTATGAC TCAGACCTGG CTGTCTCTTA TACTGACCTT 
GATAATCTCT TCAATTCTGA TGAAGATGAA CTAACACCTG GATCTAAAAA ATCAGCAAAT 
GGATCAGATG ATAAAGCCAG CTGCAAGGAA TCAAAGACAG GAAATCTGGA CCCGTTATCT 
TGCATAAGCA CTGCAGATCT TCATAAAATG TATCCTACAC CACCATCATT GGAACAACAT 
ATTATGGGAT TTTCCCCAAT GAATATGAAT AATAAAGAAT ATGGTAGTAT GGATACAACA 
CCTGGAGGAA CTGTTCTAGA AGGAAATAGT TCTAGTATAG GAGCGCAGTT CAAAATTGAG 
GTTGATGAGG GATTCTGTAG CCCCAAACCT TCTGAAATTA AAGATTTTTC TTATGTCTAT 
AAGCCTGAAA ATTGTCAAAT TCTAGTGGGA TGTTCCATGT TTGCACCTCT AAAAACTCTA 
CCAAGCCAAT ATCTGCCCCC TATCAAATTG CCAGAAGAGT GTATTTACCG TCAGAGTTGG 
ACTGTTGGAA AATTGGAATT GCTTTCTTCA GGGCCTTCAA TGCCATTCAT CAAAGAGGGT 
GATGGAAGTA ATATGGATCA AGAATATGGC ACTGCTTATA CACCTCAAAC TCATACTTCT 
TTTGGGATGC CTCCTAGCAG TGCACCTCCT AGTAACAGCG GAGCAGGAAT TCTTCCTTCT 
CCATCCACCC CTCGGTTTCC AACTCCAAGG ACTCCAAGGA CTCCTCGGAC TCCTCGTGGA 
GCTGGTGGAC CTGCTAGTGC TCAAGGTTCA GTCAAATATG AAAATTCAGA CTTGTATTCA 
CCAGCTTCTA CCCCATCTAC ATGCAGACCC CTTAATTCTG TTGAACCTGC AACTGTCCCT 
TCCATCCCTG AAGCACACAG TCTTTATGTA AACCTCATCC TTTCAGAATC AGTTATGAAT 
TTGTTTAAAG ACTGTAACTT TGATAGTTGT TGCATCTGTG TTTGCAACAT GAACATCAAG 
GGTGCCGATG TTGGAGTTTA CATTCCAGAT CCAACGCAGG AAGCACAATA TAGGTGTACC 
TGTGGCTTCA GTGCTGTCAT GAACAGAAAA TTTGGAAACA ATTCAGGATT ATTTCTTGAA 
GATGAACTAG ATATCATAGG ACGCAATACA GACTGTGGCA AAGAAGCAGA AAAACGTTTT 
GAAGCTCTCA GGGCTACCTC TGCTGAACAT GTTAATGGAG GACTAAAGGA ATCTGAAAAA 
TTATCTGATG ATTTGATATT ATTGCTACAA GATCAGTGCA CTAATTTATT TTCACCCTTT 
GGAGCAGCAG ACCAAGATCC TTTTCCTAAA AGTGGTGTAA TTAGCAATTG GGTACGTGTT 
GAAGAGCGTG ACTGTTGCAA TGACTGCTAC CTTGCATTAG AACATGGGCG TCAGTTCATG 
GATAACATGT CAGGAGGAAA AGTTGATGAA GCACTTGTGA AAAGTTCATG CTTACACCCC 
TGGTCCAAAA GAAACGATGT GAGTATGCAG TGCTCACAGG ATATACTTCG AATGCTCCTC 
TCTCTTCAGC CAGTTCTTCA GGATGCCATT CAGAAAAAAA GAACAGTAAG ACCTTGGGGT 
GTTCAGGGTC CTCTCACTTG GCAACAATTT CATAAAATGG CTGGCCGAGG CTCTTATGGA 
ACTGATGAAT CCCCAGAACC ACTGCCAATC CCCACATTTT TGTTGGGTTA TGATTATGAT 
TATCTGGTGC TTTCTCCATT TGCTCTTCCT TATTGGGAGA GACTTATGCT GGAACCCTAT 
GGATCTCAAA GAGATATAGC CTATGTTGTA CTGTGTCCAG AAAATGAAGC CTTGTTAAAT 
GGAGCAAAAA GCTTTTTTAG AGATCTTACT GCAATATATG AGTCCTGTCG ATTAGGTCAA 
CATAGACCTG TTTCTCGACT GTTAACAGAT GGGATCATGA GAGTTGGATC TACTGCATCA 
AAGAAACTAT CAGAAAAGTT GGTAGCAGAA TGGTTTTCTC AGGCAGCTGA CGGTAACAAT 
GAAGCATTTT CTAAACTCAA GCTTTATGCA CAAGTCTGCA GATATGACCT AGGTCCTTAT 
CTTGCTTCCC TGCCATTGGA CAGCTCTCTA CTTTCCCAGC CAAATTTAGT TGCCCCTACA 
AGTCAGTCTT TGATTACTCC ACCTCAGATG ACAAATACTG GAAATGCTAA TACTCCATCT 
GCCACCTTAG CATCTGCAGC GAGCAGCACT ATGACAGTGA CTTCAGGTGT TGCCATATCT 
ACTTCAGTTG CCACAGCTAA TTCAACTTTG ACCACAGCTT CAACTTCATC TTCATCATCC 
TCCAACTTGA ATAGTGGAGT ATCATCAAAT AAACTACCTT CGTTTCCACC CTTTGGCAGT 
ATGAACAGTA ATGCTGCAGG ATCCATGTCT ACACAAGCAA ATACAGTTCA GAGTGGTCAG 
CTAGGAGGGC AACAGACATC AGCTCTACAG ACAGCTGGGA TTTCTGGAGA ATCATCTTCA 
CTTCCCACTC AGCCGCATCC TGATGTGTCT GAAAGCACGA TGGATCGGGA TAAAGTGGGA 
ATCCCCACAG ATGGTGATTC ACATGCAGTC ACGTATCCAC CTGCAATTGT TGTTTATATA 
ATTGATCCTT TTACATACGA AAATACAGAC GAGAGCACTA ACTCTTCTAG TGTGTGGACA 
TTGGGGCTAC TTCGATGCTT TCTAGAAATG GTCCAGACTC TTCCTCCTCA TATCAAGAGT 
ACTGTTTCTG TACAGATTAT TCCTTGTCAG TACCTGTTGC AACCTGTGAA GCATGAAGAT 
AGAGAAATCT ATCCCCAGCA TTTAAAATCC CTGGCTTTTT CGGCCTTTAC CCAGTGTCGG 
AGGCCACTTC CAACATCAAC CAATGTGAAA ACATTGACTG GCTTTGGTCC AGGTTTAGCC 
ATGGAAACTG CCCTTAGAAG TCCTGATAGA CCAGAGTGTA TTCGACTTTA TGCACCTCCT 
TTTATTCTGG CTCCAGTGAA GGACAAACAG ACAGAGCTAG GAGAAACATT TGGAGAAGCT 
GGACAGAAAT ATAATGTTCT TTTTGTGGGA TACTGTTTAT CACATGATCA AAGGTGGATT 
CTTGCATCTT GCACAGATCT ATATGGAGAA CTTTTAGAAA CTTGTATCAT TAACATCGAT 
GTTCCAAATA GGGCTCGTCG GAAAAAAAGT TCTGCTAGAA AATTTGGTCT ACAGAAACTT 
TGGGAGTGGT GCTTAGGACT TGTACAAATG AGTTCATTGC CATGGAGAGT TGTAATTGGT 
CGTCTAGGAA GGATTGGTCA TGGAGAATTG AAAGATTGGA GCTGTTTGCT GAGTCGTCGA 
AACTTGCAGT CTCTAAGTAA AAGGCTCAAA GACATGTGTA GAATGTGTGG TATATCTGCT 
GCAGACTCCC CTAGCATTCT CAGTGCTTGC TTGGTGGCAA TGGAGCCGCA AGGCTCTTTT 
GTTATTATGC CAGATTCTGT GTCAACTGGT TCTGTATTTG GAAGAAGCAC GACTCTAAAT 
ATGCAGACAT CTCAGCTAAA TACCCCACAG GATACATCAT GTACTCATAT ACTTGTGTTT 
CCTACTTCTG CTTCTGTGCA AGTAGCTTCA GCTACTTATA CCACTGAAAA TTTGGATTTA 
GCTTTCAATC CCAACAATGA TGGAGCAGAT GGAATGGGTA TCTTTGATTT GTTAGACACA 
GGAGATGATC TTGACCCTGA TATCATTAAT ATCCTTCCTG CTTCTCCAAC TGGTTCTCCT 
GTACATTCTC CAGGATCTCA TTACCCCCAT GGAGGTGATG CGGGCAAGGG TCAGAGTACT 
GATCGGCTAC TATCAACAGA ACCTCATGAG GAAGTACCTA ATATTCTTCA GCAACCATTG 
GCCCTTGGTT ACTTTGTATC AACTGCCAAA GCAGGTCCAT TACCTGACTG GTTCTGGTCA 
GCATGTCCTC AAGCACAATA TCAGTGTCCC CTTTTTCTTA AGGCCTCTTT GCACCTCCAC 
GTGCCTTCAG TGCAATCTGA CGAGCTGCTT CACAGTAAAC ACTCCCACCC ACTTGACTCA 
AATCAGACTT CAGATGTCCT CAGGTTTGTT TTGGAACAGT ACAATGCACT CTCCTGGCTA 
ACCTGTGACC CTGCAACCCA GGACAGACGC TCATGTCTCC CAATTCATTT TGTGGTGCTG 
AATCAGTTAT ATAACTTTAT TATGAATATG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED13 ( XM_011525553.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523855.1 Homo sapiens mediator complex subunit 13 (MED13), transcript variant X3, mRNA.

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CloneID	OHu63563
Clone ID Related Accession (Same CDS sequence)	XM_011525553.3 , XM_011525553.2
Accession Version	XM_011525553.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 13 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGATT CAGCTACAAA AAAATTAATT GGTGAATGGA AACAGTTCTA TCCTATCTCA 
TGTTGCTTGA AGGAGATGTC TGAAGAAAAA CAGGAAGATA TGGATTGGGA AGATGATTCT 
TTAGCTGCAG TAGAAGTTCT TGTTGCTGGT GTCCGAATGA TCTACCCAGC ATGCTTTGTT 
CTAGTCCCTC AGTCAGACAT TCCTACTCCT AGCCCTGTGG GATCCACTCA CTGTTCATCT 
TCTTGCTTGG GTGTCCACCA AGTGCCTGCT TCCACAAGAG ATCCTGCTAT GTCTTCGGTT 
ACGCTTACAC CACCTACGTC TCCTGAGGAA GTCCAAACAG TTGATCCTCA GTCTGTCCAG 
AAGTGGGTCA AATTTTCTTC AGTATCTGAT GGCTTCAACT CCGATAGTAC TAGCCACCAT 
GGTGGGAAAA TACCCAGAAA ATTAGCAAAT CATGTGGTGG ATAGAGTTTG GCAAGAATGC 
AATATGAACA GAGCACAGAA CAAGAGGAAG TATTCTGCTT CATCAGGTGG TCTATGCGAA 
GAAGCGACAG CTGCTAAAGT GGCATCCTGG GATTTTGTTG AAGCCACACA AAGAACAAAT 
TGCAGTTGTT TGAGGCACAA AAATCTCAAG TCAAGAAATG CTGGACAACA AGGACAGGCA 
CCATCTTTAG GTCAGCAACA ACAAATACTT CCTAAGCACA AGACCAATGA GAAGCAAGAA 
AAGAGTGAAA AGCCACAGAA ACGCCCCTTG ACTCCTTTTC ACCATCGTGT GTCTGTTAGT 
GATGATGTTG GCATGGACGC AGATTCAGCC AGCCAAAGAC TTGTGATCTC TGCTCCAGAC 
AGTCAAGTGA GATTTTCAAA TATCCGAACT AATGATGTAG CAAAGACTCC TCAGATGCAT 
GGCACCGAAA TGGCAAATTC ACCTCAACCA CCCCCACTTA GTCCTCACCC TTGTGATGTG 
GTTGATGAAG GAGTGACTAA AACACCTTCA ACTCCTCAGA GTCAACATTT TTATCAAATG 
CCAACACCAG ATCCCTTGGT TCCTTCTAAA CCAATGGAAG ATAGGATAGA CAGTTTGTCC 
CAGTCTTTCC CACCTCAATA TCAGGAAGCT GTAGAACCTA CAGTATATGT TGGTACAGCA 
GTAAACTTGG AAGAAGATGA AGCCAATATA GCCTGGAAGT ATTACAAGTT CCCAAAGAAA 
AAAGATGTAG AGTTTTTACC ACCTCAACTT CCAAGTGATA AATTCAAGGA TGATCCAGTT 
GGACCTTTTG GACAGGAAAG TGTAACATCA GTTACAGAGT TAATGGTGCA ATGTAAGAAA 
CCTTTAAAAG TTTCTGATGA ATTAGTGCAG CAATATCAAA TTAAAAACCA GTGTCTTTCA 
GCAATAGCAT CTGATGCAGA ACAAGAACCT AAAATTGATC CATATGCATT TGTTGAAGGA 
GATGAGGAAT TCCTTTTTCC TGATAAAAAA GATAGACAAA ATAGTGAGAG AGAAGCTGGA 
AAAAAACACA AGGTAGAAGA TGGGACATCT AGTGTAACAG TGTTATCACA TGAAGAAGAT 
GCTATGTCAT TATTTAGTCC CTCTATCAAG CAAGATGCTC CACGCCCTAC TAGTCATGCC 
CGTCCTCCAT CAACAAGTTT GATTTATGAC TCAGACCTGG CTGTCTCTTA TACTGACCTT 
GATAATCTCT TCAATTCTGA TGAAGATGAA CTAACACCTG GATCTAAAAA ATCAGCAAAT 
GGATCAGATG ATAAAGCCAG CTGCAAGGAA TCAAAGACAG GAAATCTGGA CCCGTTATCT 
TGCATAAGCA CTGCAGATCT TCATAAAATG TATCCTACAC CACCATCATT GGAACAACAT 
ATTATGGGAT TTTCCCCAAT GAATATGAAT AATAAAGAAT ATGGTAGTAT GGATACAACA 
CCTGGAGGAA CTGTTCTAGA AGGAAATAGT TCTAGTATAG GAGCGCAGTT CAAAATTGAG 
GTTGATGAGG GATTCTGTAG CCCCAAACCT TCTGAAATTA AAGATTTTTC TTATGTCTAT 
AAGCCTGAAA ATTGTCAAAT TCTAGTGGGA TGTTCCATGT TTGCACCTCT AAAAACTCTA 
CCAAGCCAAT ATCTGCCCCC TATCAAATTG CCAGAAGAGT GTATTTACCG TCAGAGTTGG 
ACTGTTGGAA AATTGGAATT GCTTTCTTCA GGGCCTTCAA TGCCATTCAT CAAAGAGGGT 
GATGGAAGTA ATATGGATCA AGAATATGGC ACTGCTTATA CACCTCAAAC TCATACTTCT 
TTTGGGATGC CTCCTAGCAG TGCACCTCCT AGTAACAGCG GAGCAGGAAT TCTTCCTTCT 
CCATCCACCC CTCGGTTTCC AACTCCAAGG ACTCCAAGGA CTCCTCGGAC TCCTCGTGGA 
GCTGGTGGAC CTGCTAGTGC TCAAGGTTCA GTCAAATATG AAAATTCAGA CTTGTATTCA 
CCAGCTTCTA CCCCATCTAC ATGCAGACCC CTTAATTCTG TTGAACCTGC AACTGTCCCT 
TCCATCCCTG AAGCACACAG TCTTTATGTA AACCTCATCC TTTCAGAATC AGTTATGAAT 
TTGTTTAAAG ACTGTAACTT TGATAGTTGT TGCATCTGTG TTTGCAACAT GAACATCAAG 
GGTGCCGATG TTGGAGTTTA CATTCCAGAT CCAACGCAGG AAGCACAATA TAGGTGTACC 
TGTGGCTTCA GTGCTGTCAT GAACAGAAAA TTTGGAAACA ATTCAGGATT ATTTCTTGAA 
GATGAACTAG ATATCATAGG ACGCAATACA GACTGTGGCA AAGAAGCAGA AAAACGTTTT 
GAAGCTCTCA GGGCTACCTC TGCTGAACAT GTTAATGGAG GACTAAAGGA ATCTGAAAAA 
TTATCTGATG ATTTGATATT ATTGCTACAA GATCAGTGCA CTAATTTATT TTCACCCTTT 
GGAGCAGCAG ACCAAGATCC TTTTCCTAAA AGTGGTGTAA TTAGCAATTG GGTACGTGTT 
GAAGAGCGTG ACTGTTGCAA TGACTGCTAC CTTGCATTAG AACATGGGCG TCAGTTCATG 
GATAACATGT CAGGAGGAAA AGTTGATGAA GCACTTGTGA AAAGTTCATG CTTACACCCC 
TGGTCCAAAA GAAACGATGT GAGTATGCAG TGCTCACAGG ATATACTTCG AATGCTCCTC 
TCTCTTCAGC CAGTTCTTCA GGATGCCATT CAGAAAAAAA GAACAGTAAG ACCTTGGGGT 
GTTCAGGGTC CTCTCACTTG GCAACAATTT CATAAAATGG CTGGCCGAGG CTCTTATGGA 
ACTGATGAAT CCCCAGAACC ACTGCCAATC CCCACATTTT TGTTGGGTTA TGATTATGAT 
TATCTGGTGC TTTCTCCATT TGCTCTTCCT TATTGGGAGA GACTTATGCT GGAACCCTAT 
GGATCTCAAA GAGATATAGC CTATGTTGTA CTGTGTCCAG AAAATGAAGC CTTGTTAAAT 
GGAGCAAAAA GCTTTTTTAG AGATCTTACT GCAATATATG AGTCCTGTCG ATTAGGTCAA 
CATAGACCTG TTTCTCGACT GTTAACAGAT GGGATCATGA GAGTTGGATC TACTGCATCA 
AAGAAACTAT CAGAAAAGTT GGTAGCAGAA TGGTTTTCTC AGGCAGCTGA CGGTAACAAT 
GAAGCATTTT CTAAACTCAA GCTTTATGCA CAAGTCTGCA GATATGACCT AGGTCCTTAT 
CTTGCTTCCC TGCCATTGGA CAGCTCTCTA CTTTCCCAGC CAAATTTAGT TGCCCCTACA 
AGTCAGTCTT TGATTACTCC ACCTCAGATG ACAAATACTG GAAATGCTAA TACTCCATCT 
GCCACCTTAG CATCTGCAGC GAGCAGCACT ATGACAGTGA CTTCAGGTGT TGCCATATCT 
ACTTCAGTTG CCACAGCTAA TTCAACTTTG ACCACAGCTT CAACTTCATC TTCATCATCC 
TCCAACTTGA ATAGTGGAGT ATCATCAAAT AAACTACCTT CGTTTCCACC CTTTGGCAGT 
ATGAACAGTA ATGCTGCAGG ATCCATGTCT ACACAAGCAA ATACAGTTCA GAGTGGTCAG 
CTAGGAGGGC AACAGACATC AGCTCTACAG ACAGCTGGGA TTTCTGGAGA ATCATCTTCA 
CTTCCCACTC AGCCGCATCC TGATGTGTCT GAAAGCACGA TGGATCGGGA TAAAGTGGGA 
ATCCCCACAG ATGGTGATTC ACATGCAGTC ACGTATCCAC CTGCAATTGT TGTTTATATA 
ATTGATCCTT TTACATACGA AAATACAGAC GAGAGCACTA ACTCTTCTAG TGTGTGGACA 
TTGGGGCTAC TTCGATGCTT TCTAGAAATG GTCCAGACTC TTCCTCCTCA TATCAAGAGT 
ACTGTTTCTG TACAGATTAT TCCTTGTCAG TACCTGTTGC AACCTGTGAA GCATGAAGAT 
AGAGAAATCT ATCCCCAGCA TTTAAAATCC CTGGCTTTTT CGGCCTTTAC CCAGTGTCGG 
AGGCCACTTC CAACATCAAC CAATGTGAAA ACATTGACTG GCTTTGGTCC AGGTTTAGCC 
ATGGAAACTG CCCTTAGAAG TCCTGATAGA CCAGAGTGTA TTCGACTTTA TGCACCTCCT 
TTTATTCTGG CTCCAGTGAA GGACAAACAG ACAGAGCTAG GAGAAACATT TGGAGAAGCT 
GGACAGAAAT ATAATGTTCT TTTTGTGGGA TACTGTTTAT CACATGATCA AAGGTGGATT 
CTTGCATCTT GCACAGATCT ATATGGAGAA CTTTTAGAAA CTTGTATCAT TAACATCGAT 
GTTCCAAATA GGGCTCGTCG GAAAAAAAGT TCTGCTAGAA AATTTGGTCT ACAGAAACTT 
TGGGAGTGGT GCTTAGGACT TGTACAAATG AGTTCATTGC CATGGAGAGT TGTAATTGGT 
CGTCTAGGAA GGATTGGTCA TGGAGAATTG AAAGATTGGA GCTGTTTGCT GAGTCGTCGA 
AACTTGCAGT CTCTAAGTAA AAGGCTCAAA GACATGTGTA GAATGTGTGG TATATCTGCT 
GCAGACTCCC CTAGCATTCT CAGTGCTTGC TTGGTGGCAA TGGAGCCGCA AGGCTCTTTT 
GTTATTATGC CAGATTCTGT GTCAACTGGT TCTGTATTTG GAAGAAGCAC GACTCTAAAT 
ATGCAGACAT CTCAGCTAAA TACCCCACAG GATACATCAT GTACTCATAT ACTTGTGTTT 
CCTACTTCTG CTTCTGTGCA AGTAGCTTCA GCTACTTATA CCACTGAAAA TTTGGATTTA 
GCTTTCAATC CCAACAATGA TGGAGCAGAT GGAATGGGTA TCTTTGATTT GTTAGACACA 
GGAGATGATC TTGACCCTGA TATCATTAAT ATCCTTCCTG CTTCTCCAAC TGGTTCTCCT 
GTACATTCTC CAGGATCTCA TTACCCCCAT GGAGGTGATG CGGGCAAGGG TCAGAGTACT 
GATCGGCTAC TATCAACAGA ACCTCATGAG GAAGTACCTA ATATTCTTCA GCAACCATTG 
GCCCTTGGTT ACTTTGTATC AACTGCCAAA GCAGGTCCAT TACCTGACTG GTTCTGGTCA 
GCATGTCCTC AAGCACAATA TCAGTGTCCC CTTTTTCTTA AGGCCTCTTT GCACCTCCAC 
GTGCCTTCAG TGCAATCTGA CGAGCTGCTT CACAGTAAAC ACTCCCACCC ACTTGACTCA 
AATCAGACTT CAGATGTCCT CAGGTTTGTT TTGGAACAGT ACAATGCACT CTCCTGGCTA 
ACCTGTGACC CTGCAACCCA GGACAGACGC TCATGTCTCC CAATTCATTT TGTGGTGCTG 
AATCAGTTAT ATAACTTTAT TATGAATATG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523855.1
CDS	162..6017
Translation	MSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGCSMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHPDVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML

Target ORF information:

RefSeq Version	XM_011525553.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525553.2

ORF Insert Sequence:

ATGTCTGATT CAGCTACAAA AAAATTAATT GGTGAATGGA AACAGTTCTA TCCTATCTCA 
TGTTGCTTGA AGGAGATGTC TGAAGAAAAA CAGGAAGATA TGGATTGGGA AGATGATTCT 
TTAGCTGCAG TAGAAGTTCT TGTTGCTGGT GTCCGAATGA TCTACCCAGC ATGCTTTGTT 
CTAGTCCCTC AGTCAGACAT TCCTACTCCT AGCCCTGTGG GATCCACTCA CTGTTCATCT 
TCTTGCTTGG GTGTCCACCA AGTGCCTGCT TCCACAAGAG ATCCTGCTAT GTCTTCGGTT 
ACGCTTACAC CACCTACGTC TCCTGAGGAA GTCCAAACAG TTGATCCTCA GTCTGTCCAG 
AAGTGGGTCA AATTTTCTTC AGTATCTGAT GGCTTCAACT CCGATAGTAC TAGCCACCAT 
GGTGGGAAAA TACCCAGAAA ATTAGCAAAT CATGTGGTGG ATAGAGTTTG GCAAGAATGC 
AATATGAACA GAGCACAGAA CAAGAGGAAG TATTCTGCTT CATCAGGTGG TCTATGCGAA 
GAAGCGACAG CTGCTAAAGT GGCATCCTGG GATTTTGTTG AAGCCACACA AAGAACAAAT 
TGCAGTTGTT TGAGGCACAA AAATCTCAAG TCAAGAAATG CTGGACAACA AGGACAGGCA 
CCATCTTTAG GTCAGCAACA ACAAATACTT CCTAAGCACA AGACCAATGA GAAGCAAGAA 
AAGAGTGAAA AGCCACAGAA ACGCCCCTTG ACTCCTTTTC ACCATCGTGT GTCTGTTAGT 
GATGATGTTG GCATGGACGC AGATTCAGCC AGCCAAAGAC TTGTGATCTC TGCTCCAGAC 
AGTCAAGTGA GATTTTCAAA TATCCGAACT AATGATGTAG CAAAGACTCC TCAGATGCAT 
GGCACCGAAA TGGCAAATTC ACCTCAACCA CCCCCACTTA GTCCTCACCC TTGTGATGTG 
GTTGATGAAG GAGTGACTAA AACACCTTCA ACTCCTCAGA GTCAACATTT TTATCAAATG 
CCAACACCAG ATCCCTTGGT TCCTTCTAAA CCAATGGAAG ATAGGATAGA CAGTTTGTCC 
CAGTCTTTCC CACCTCAATA TCAGGAAGCT GTAGAACCTA CAGTATATGT TGGTACAGCA 
GTAAACTTGG AAGAAGATGA AGCCAATATA GCCTGGAAGT ATTACAAGTT CCCAAAGAAA 
AAAGATGTAG AGTTTTTACC ACCTCAACTT CCAAGTGATA AATTCAAGGA TGATCCAGTT 
GGACCTTTTG GACAGGAAAG TGTAACATCA GTTACAGAGT TAATGGTGCA ATGTAAGAAA 
CCTTTAAAAG TTTCTGATGA ATTAGTGCAG CAATATCAAA TTAAAAACCA GTGTCTTTCA 
GCAATAGCAT CTGATGCAGA ACAAGAACCT AAAATTGATC CATATGCATT TGTTGAAGGA 
GATGAGGAAT TCCTTTTTCC TGATAAAAAA GATAGACAAA ATAGTGAGAG AGAAGCTGGA 
AAAAAACACA AGGTAGAAGA TGGGACATCT AGTGTAACAG TGTTATCACA TGAAGAAGAT 
GCTATGTCAT TATTTAGTCC CTCTATCAAG CAAGATGCTC CACGCCCTAC TAGTCATGCC 
CGTCCTCCAT CAACAAGTTT GATTTATGAC TCAGACCTGG CTGTCTCTTA TACTGACCTT 
GATAATCTCT TCAATTCTGA TGAAGATGAA CTAACACCTG GATCTAAAAA ATCAGCAAAT 
GGATCAGATG ATAAAGCCAG CTGCAAGGAA TCAAAGACAG GAAATCTGGA CCCGTTATCT 
TGCATAAGCA CTGCAGATCT TCATAAAATG TATCCTACAC CACCATCATT GGAACAACAT 
ATTATGGGAT TTTCCCCAAT GAATATGAAT AATAAAGAAT ATGGTAGTAT GGATACAACA 
CCTGGAGGAA CTGTTCTAGA AGGAAATAGT TCTAGTATAG GAGCGCAGTT CAAAATTGAG 
GTTGATGAGG GATTCTGTAG CCCCAAACCT TCTGAAATTA AAGATTTTTC TTATGTCTAT 
AAGCCTGAAA ATTGTCAAAT TCTAGTGGGA TGTTCCATGT TTGCACCTCT AAAAACTCTA 
CCAAGCCAAT ATCTGCCCCC TATCAAATTG CCAGAAGAGT GTATTTACCG TCAGAGTTGG 
ACTGTTGGAA AATTGGAATT GCTTTCTTCA GGGCCTTCAA TGCCATTCAT CAAAGAGGGT 
GATGGAAGTA ATATGGATCA AGAATATGGC ACTGCTTATA CACCTCAAAC TCATACTTCT 
TTTGGGATGC CTCCTAGCAG TGCACCTCCT AGTAACAGCG GAGCAGGAAT TCTTCCTTCT 
CCATCCACCC CTCGGTTTCC AACTCCAAGG ACTCCAAGGA CTCCTCGGAC TCCTCGTGGA 
GCTGGTGGAC CTGCTAGTGC TCAAGGTTCA GTCAAATATG AAAATTCAGA CTTGTATTCA 
CCAGCTTCTA CCCCATCTAC ATGCAGACCC CTTAATTCTG TTGAACCTGC AACTGTCCCT 
TCCATCCCTG AAGCACACAG TCTTTATGTA AACCTCATCC TTTCAGAATC AGTTATGAAT 
TTGTTTAAAG ACTGTAACTT TGATAGTTGT TGCATCTGTG TTTGCAACAT GAACATCAAG 
GGTGCCGATG TTGGAGTTTA CATTCCAGAT CCAACGCAGG AAGCACAATA TAGGTGTACC 
TGTGGCTTCA GTGCTGTCAT GAACAGAAAA TTTGGAAACA ATTCAGGATT ATTTCTTGAA 
GATGAACTAG ATATCATAGG ACGCAATACA GACTGTGGCA AAGAAGCAGA AAAACGTTTT 
GAAGCTCTCA GGGCTACCTC TGCTGAACAT GTTAATGGAG GACTAAAGGA ATCTGAAAAA 
TTATCTGATG ATTTGATATT ATTGCTACAA GATCAGTGCA CTAATTTATT TTCACCCTTT 
GGAGCAGCAG ACCAAGATCC TTTTCCTAAA AGTGGTGTAA TTAGCAATTG GGTACGTGTT 
GAAGAGCGTG ACTGTTGCAA TGACTGCTAC CTTGCATTAG AACATGGGCG TCAGTTCATG 
GATAACATGT CAGGAGGAAA AGTTGATGAA GCACTTGTGA AAAGTTCATG CTTACACCCC 
TGGTCCAAAA GAAACGATGT GAGTATGCAG TGCTCACAGG ATATACTTCG AATGCTCCTC 
TCTCTTCAGC CAGTTCTTCA GGATGCCATT CAGAAAAAAA GAACAGTAAG ACCTTGGGGT 
GTTCAGGGTC CTCTCACTTG GCAACAATTT CATAAAATGG CTGGCCGAGG CTCTTATGGA 
ACTGATGAAT CCCCAGAACC ACTGCCAATC CCCACATTTT TGTTGGGTTA TGATTATGAT 
TATCTGGTGC TTTCTCCATT TGCTCTTCCT TATTGGGAGA GACTTATGCT GGAACCCTAT 
GGATCTCAAA GAGATATAGC CTATGTTGTA CTGTGTCCAG AAAATGAAGC CTTGTTAAAT 
GGAGCAAAAA GCTTTTTTAG AGATCTTACT GCAATATATG AGTCCTGTCG ATTAGGTCAA 
CATAGACCTG TTTCTCGACT GTTAACAGAT GGGATCATGA GAGTTGGATC TACTGCATCA 
AAGAAACTAT CAGAAAAGTT GGTAGCAGAA TGGTTTTCTC AGGCAGCTGA CGGTAACAAT 
GAAGCATTTT CTAAACTCAA GCTTTATGCA CAAGTCTGCA GATATGACCT AGGTCCTTAT 
CTTGCTTCCC TGCCATTGGA CAGCTCTCTA CTTTCCCAGC CAAATTTAGT TGCCCCTACA 
AGTCAGTCTT TGATTACTCC ACCTCAGATG ACAAATACTG GAAATGCTAA TACTCCATCT 
GCCACCTTAG CATCTGCAGC GAGCAGCACT ATGACAGTGA CTTCAGGTGT TGCCATATCT 
ACTTCAGTTG CCACAGCTAA TTCAACTTTG ACCACAGCTT CAACTTCATC TTCATCATCC 
TCCAACTTGA ATAGTGGAGT ATCATCAAAT AAACTACCTT CGTTTCCACC CTTTGGCAGT 
ATGAACAGTA ATGCTGCAGG ATCCATGTCT ACACAAGCAA ATACAGTTCA GAGTGGTCAG 
CTAGGAGGGC AACAGACATC AGCTCTACAG ACAGCTGGGA TTTCTGGAGA ATCATCTTCA 
CTTCCCACTC AGCCGCATCC TGATGTGTCT GAAAGCACGA TGGATCGGGA TAAAGTGGGA 
ATCCCCACAG ATGGTGATTC ACATGCAGTC ACGTATCCAC CTGCAATTGT TGTTTATATA 
ATTGATCCTT TTACATACGA AAATACAGAC GAGAGCACTA ACTCTTCTAG TGTGTGGACA 
TTGGGGCTAC TTCGATGCTT TCTAGAAATG GTCCAGACTC TTCCTCCTCA TATCAAGAGT 
ACTGTTTCTG TACAGATTAT TCCTTGTCAG TACCTGTTGC AACCTGTGAA GCATGAAGAT 
AGAGAAATCT ATCCCCAGCA TTTAAAATCC CTGGCTTTTT CGGCCTTTAC CCAGTGTCGG 
AGGCCACTTC CAACATCAAC CAATGTGAAA ACATTGACTG GCTTTGGTCC AGGTTTAGCC 
ATGGAAACTG CCCTTAGAAG TCCTGATAGA CCAGAGTGTA TTCGACTTTA TGCACCTCCT 
TTTATTCTGG CTCCAGTGAA GGACAAACAG ACAGAGCTAG GAGAAACATT TGGAGAAGCT 
GGACAGAAAT ATAATGTTCT TTTTGTGGGA TACTGTTTAT CACATGATCA AAGGTGGATT 
CTTGCATCTT GCACAGATCT ATATGGAGAA CTTTTAGAAA CTTGTATCAT TAACATCGAT 
GTTCCAAATA GGGCTCGTCG GAAAAAAAGT TCTGCTAGAA AATTTGGTCT ACAGAAACTT 
TGGGAGTGGT GCTTAGGACT TGTACAAATG AGTTCATTGC CATGGAGAGT TGTAATTGGT 
CGTCTAGGAA GGATTGGTCA TGGAGAATTG AAAGATTGGA GCTGTTTGCT GAGTCGTCGA 
AACTTGCAGT CTCTAAGTAA AAGGCTCAAA GACATGTGTA GAATGTGTGG TATATCTGCT 
GCAGACTCCC CTAGCATTCT CAGTGCTTGC TTGGTGGCAA TGGAGCCGCA AGGCTCTTTT 
GTTATTATGC CAGATTCTGT GTCAACTGGT TCTGTATTTG GAAGAAGCAC GACTCTAAAT 
ATGCAGACAT CTCAGCTAAA TACCCCACAG GATACATCAT GTACTCATAT ACTTGTGTTT 
CCTACTTCTG CTTCTGTGCA AGTAGCTTCA GCTACTTATA CCACTGAAAA TTTGGATTTA 
GCTTTCAATC CCAACAATGA TGGAGCAGAT GGAATGGGTA TCTTTGATTT GTTAGACACA 
GGAGATGATC TTGACCCTGA TATCATTAAT ATCCTTCCTG CTTCTCCAAC TGGTTCTCCT 
GTACATTCTC CAGGATCTCA TTACCCCCAT GGAGGTGATG CGGGCAAGGG TCAGAGTACT 
GATCGGCTAC TATCAACAGA ACCTCATGAG GAAGTACCTA ATATTCTTCA GCAACCATTG 
GCCCTTGGTT ACTTTGTATC AACTGCCAAA GCAGGTCCAT TACCTGACTG GTTCTGGTCA 
GCATGTCCTC AAGCACAATA TCAGTGTCCC CTTTTTCTTA AGGCCTCTTT GCACCTCCAC 
GTGCCTTCAG TGCAATCTGA CGAGCTGCTT CACAGTAAAC ACTCCCACCC ACTTGACTCA 
AATCAGACTT CAGATGTCCT CAGGTTTGTT TTGGAACAGT ACAATGCACT CTCCTGGCTA 
ACCTGTGACC CTGCAACCCA GGACAGACGC TCATGTCTCC CAATTCATTT TGTGGTGCTG 
AATCAGTTAT ATAACTTTAT TATGAATATG CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED13 ( NM_005121.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005112.2 Homo sapiens mediator complex subunit 13 (MED13), mRNA.

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CloneID	OHu04704
Clone ID Related Accession (Same CDS sequence)	NM_005121.2 , NM_005121.3
Accession Version	NM_005121.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP332524.1, BQ709424.1, AF117754.1, AB011165.2, AC060798.10 and CN273870.1. This sequence is a reference standard in the RefSeqGene project. On May 26, 2006 this sequence version replaced NM_005121.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117754.1, BC140891.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTGGAG CTGTTTGCTG 
AGTCGTCGAA ACTTGCAGTC TCTAAGTAAA AGGCTCAAAG ACATGTGTAG AATGTGTGGT 
ATATCTGCTG CAGACTCCCC TAGCATTCTC AGTGCTTGCT TGGTGGCAAT GGAGCCGCAA 
GGCTCTTTTG TTATTATGCC AGATTCTGTG TCAACTGGTT CTGTATTTGG AAGAAGCACG 
ACTCTAAATA TGCAGACATC TCAGCTAAAT ACCCCACAGG ATACATCATG TACTCATATA 
CTTGTGTTTC CTACTTCTGC TTCTGTGCAA GTAGCTTCAG CTACTTATAC CACTGAAAAT 
TTGGATTTAG CTTTCAATCC CAACAATGAT GGAGCAGATG GAATGGGTAT CTTTGATTTG 
TTAGACACAG GAGATGATCT TGACCCTGAT ATCATTAATA TCCTTCCTGC TTCTCCAACT 
GGTTCTCCTG TACATTCTCC AGGATCTCAT TACCCCCATG GAGGTGATGC GGGCAAGGGT 
CAGAGTACTG ATCGGCTACT ATCAACAGAA CCTCATGAGG AAGTACCTAA TATTCTTCAG 
CAACCATTGG CCCTTGGTTA CTTTGTATCA ACTGCCAAAG CAGGTCCATT ACCTGACTGG 
TTCTGGTCAG CATGTCCTCA AGCACAATAT CAGTGTCCCC TTTTTCTTAA GGCCTCTTTG 
CACCTCCACG TGCCTTCAGT GCAATCTGAC GAGCTGCTTC ACAGTAAACA CTCCCACCCA 
CTTGACTCAA ATCAGACTTC AGATGTCCTC AGGTTTGTTT TGGAACAGTA CAATGCACTC 
TCCTGGCTAA CCTGTGACCC TGCAACCCAG GACAGACGCT CATGTCTCCC AATTCATTTT 
GTGGTGCTGA ATCAGTTATA TAACTTTATT ATGAATATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005112.2
CDS	78..6602
Translation	MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPSFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGCSMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHPDVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML

Target ORF information:

RefSeq Version	NM_005121.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 13 (MED13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005121.2

ORF Insert Sequence:

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTGGAG CTGTTTGCTG 
AGTCGTCGAA ACTTGCAGTC TCTAAGTAAA AGGCTCAAAG ACATGTGTAG AATGTGTGGT 
ATATCTGCTG CAGACTCCCC TAGCATTCTC AGTGCTTGCT TGGTGGCAAT GGAGCCGCAA 
GGCTCTTTTG TTATTATGCC AGATTCTGTG TCAACTGGTT CTGTATTTGG AAGAAGCACG 
ACTCTAAATA TGCAGACATC TCAGCTAAAT ACCCCACAGG ATACATCATG TACTCATATA 
CTTGTGTTTC CTACTTCTGC TTCTGTGCAA GTAGCTTCAG CTACTTATAC CACTGAAAAT 
TTGGATTTAG CTTTCAATCC CAACAATGAT GGAGCAGATG GAATGGGTAT CTTTGATTTG 
TTAGACACAG GAGATGATCT TGACCCTGAT ATCATTAATA TCCTTCCTGC TTCTCCAACT 
GGTTCTCCTG TACATTCTCC AGGATCTCAT TACCCCCATG GAGGTGATGC GGGCAAGGGT 
CAGAGTACTG ATCGGCTACT ATCAACAGAA CCTCATGAGG AAGTACCTAA TATTCTTCAG 
CAACCATTGG CCCTTGGTTA CTTTGTATCA ACTGCCAAAG CAGGTCCATT ACCTGACTGG 
TTCTGGTCAG CATGTCCTCA AGCACAATAT CAGTGTCCCC TTTTTCTTAA GGCCTCTTTG 
CACCTCCACG TGCCTTCAGT GCAATCTGAC GAGCTGCTTC ACAGTAAACA CTCCCACCCA 
CTTGACTCAA ATCAGACTTC AGATGTCCTC AGGTTTGTTT TGGAACAGTA CAATGCACTC 
TCCTGGCTAA CCTGTGACCC TGCAACCCAG GACAGACGCT CATGTCTCCC AATTCATTTT 
GTGGTGCTGA ATCAGTTATA TAACTTTATT ATGAATATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED13 ( NM_005121.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005112.2 Homo sapiens mediator complex subunit 13 (MED13), mRNA.

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CloneID	OHu04704
Clone ID Related Accession (Same CDS sequence)	NM_005121.2 , NM_005121.3
Accession Version	NM_005121.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6525bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 13
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP332524.1, BQ709424.1, AF117754.1, AB011165.2, AC060798.10 and CN273870.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005121.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF117754.1, BC140891.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397786.7/ ENSP00000380888.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTGGAG CTGTTTGCTG 
AGTCGTCGAA ACTTGCAGTC TCTAAGTAAA AGGCTCAAAG ACATGTGTAG AATGTGTGGT 
ATATCTGCTG CAGACTCCCC TAGCATTCTC AGTGCTTGCT TGGTGGCAAT GGAGCCGCAA 
GGCTCTTTTG TTATTATGCC AGATTCTGTG TCAACTGGTT CTGTATTTGG AAGAAGCACG 
ACTCTAAATA TGCAGACATC TCAGCTAAAT ACCCCACAGG ATACATCATG TACTCATATA 
CTTGTGTTTC CTACTTCTGC TTCTGTGCAA GTAGCTTCAG CTACTTATAC CACTGAAAAT 
TTGGATTTAG CTTTCAATCC CAACAATGAT GGAGCAGATG GAATGGGTAT CTTTGATTTG 
TTAGACACAG GAGATGATCT TGACCCTGAT ATCATTAATA TCCTTCCTGC TTCTCCAACT 
GGTTCTCCTG TACATTCTCC AGGATCTCAT TACCCCCATG GAGGTGATGC GGGCAAGGGT 
CAGAGTACTG ATCGGCTACT ATCAACAGAA CCTCATGAGG AAGTACCTAA TATTCTTCAG 
CAACCATTGG CCCTTGGTTA CTTTGTATCA ACTGCCAAAG CAGGTCCATT ACCTGACTGG 
TTCTGGTCAG CATGTCCTCA AGCACAATAT CAGTGTCCCC TTTTTCTTAA GGCCTCTTTG 
CACCTCCACG TGCCTTCAGT GCAATCTGAC GAGCTGCTTC ACAGTAAACA CTCCCACCCA 
CTTGACTCAA ATCAGACTTC AGATGTCCTC AGGTTTGTTT TGGAACAGTA CAATGCACTC 
TCCTGGCTAA CCTGTGACCC TGCAACCCAG GACAGACGCT CATGTCTCCC AATTCATTTT 
GTGGTGCTGA ATCAGTTATA TAACTTTATT ATGAATATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005112.2
CDS	74..6598
Translation	MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPSFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGCSMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHPDVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLKDMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML

Target ORF information:

RefSeq Version	NM_005121.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 13 (MED13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005121.3

ORF Insert Sequence:

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTGGAG CTGTTTGCTG 
AGTCGTCGAA ACTTGCAGTC TCTAAGTAAA AGGCTCAAAG ACATGTGTAG AATGTGTGGT 
ATATCTGCTG CAGACTCCCC TAGCATTCTC AGTGCTTGCT TGGTGGCAAT GGAGCCGCAA 
GGCTCTTTTG TTATTATGCC AGATTCTGTG TCAACTGGTT CTGTATTTGG AAGAAGCACG 
ACTCTAAATA TGCAGACATC TCAGCTAAAT ACCCCACAGG ATACATCATG TACTCATATA 
CTTGTGTTTC CTACTTCTGC TTCTGTGCAA GTAGCTTCAG CTACTTATAC CACTGAAAAT 
TTGGATTTAG CTTTCAATCC CAACAATGAT GGAGCAGATG GAATGGGTAT CTTTGATTTG 
TTAGACACAG GAGATGATCT TGACCCTGAT ATCATTAATA TCCTTCCTGC TTCTCCAACT 
GGTTCTCCTG TACATTCTCC AGGATCTCAT TACCCCCATG GAGGTGATGC GGGCAAGGGT 
CAGAGTACTG ATCGGCTACT ATCAACAGAA CCTCATGAGG AAGTACCTAA TATTCTTCAG 
CAACCATTGG CCCTTGGTTA CTTTGTATCA ACTGCCAAAG CAGGTCCATT ACCTGACTGG 
TTCTGGTCAG CATGTCCTCA AGCACAATAT CAGTGTCCCC TTTTTCTTAA GGCCTCTTTG 
CACCTCCACG TGCCTTCAGT GCAATCTGAC GAGCTGCTTC ACAGTAAACA CTCCCACCCA 
CTTGACTCAA ATCAGACTTC AGATGTCCTC AGGTTTGTTT TGGAACAGTA CAATGCACTC 
TCCTGGCTAA CCTGTGACCC TGCAACCCAG GACAGACGCT CATGTCTCCC AATTCATTTT 
GTGGTGCTGA ATCAGTTATA TAACTTTATT ATGAATATGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED13 ( XM_011525551.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523853.1 Homo sapiens mediator complex subunit 13 (MED13), transcript variant X1, mRNA.

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CloneID	OHu63561
Clone ID Related Accession (Same CDS sequence)	XM_011525551.2
Accession Version	XM_011525551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 13 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525551.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTCTGT GTCAACTGGT 
TCTGTATTTG GAAGAAGCAC GACTCTAAAT ATGCAGACAT CTCAGCTAAA TACCCCACAG 
GATACATCAT GTACTCATAT ACTTGTGTTT CCTACTTCTG CTTCTGTGCA AGTAGCTTCA 
GCTACTTATA CCACTGAAAA TTTGGATTTA GCTTTCAATC CCAACAATGA TGGAGCAGAT 
GGAATGGGTA TCTTTGATTT GTTAGACACA GGAGATGATC TTGACCCTGA TATCATTAAT 
ATCCTTCCTG CTTCTCCAAC TGGTTCTCCT GTACATTCTC CAGGATCTCA TTACCCCCAT 
GGAGGTGATG CGGGCAAGGG TCAGAGTACT GATCGGCTAC TATCAACAGA ACCTCATGAG 
GAAGTACCTA ATATTCTTCA GCAACCATTG GCCCTTGGTT ACTTTGTATC AACTGCCAAA 
GCAGGTCCAT TACCTGACTG GTTCTGGTCA GCATGTCCTC AAGCACAATA TCAGTGTCCC 
CTTTTTCTTA AGGCCTCTTT GCACCTCCAC GTGCCTTCAG TGCAATCTGA CGAGCTGCTT 
CACAGTAAAC ACTCCCACCC ACTTGACTCA AATCAGACTT CAGATGTCCT CAGGTTTGTT 
TTGGAACAGT ACAATGCACT CTCCTGGCTA ACCTGTGACC CTGCAACCCA GGACAGACGC 
TCATGTCTCC CAATTCATTT TGTGGTGCTG AATCAGTTAT ATAACTTTAT TATGAATATG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523853.1
CDS	779..7144
Translation	MSASFVPNGASLEDCHCNLFCLADLTGIKWKKYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWRRDQRPGRRELWIFWWGEDPSFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIGKWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHITLAQQSNSPFQVILCPFGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCCLKEMSEEKQEDMDWEDDSLAAVEVLVAGVRMIYPACFVLVPQSDIPTPSPVGSTHCSSSCLGVHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSVQKWVKFSSVSDGFNSDSTSHHGGKIPRKLANHVVDRVWQECNMNRAQNKRKYSASSGGLCEEATAAKVASWDFVEATQRTNCSCLRHKNLKSRNAGQQGQAPSLGQQQQILPKHKTNEKQEKSEKPQKRPLTPFHHRVSVSDDVGMDADSASQRLVISAPDSQVRFSNIRTNDVAKTPQMHGTEMANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHFYQMPTPDPLVPSKPMEDRIDSLSQSFPPQYQEAVEPTVYVGTAVNLEEDEANIAWKYYKFPKKKDVEFLPPQLPSDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDELVQQYQIKNQCLSAIASDAEQEPKIDPYAFVEGDEEFLFPDKKDRQNSEREAGKKHKVEDGTSSVTVLSHEEDAMSLFSPSIKQDAPRPTSHARPPSTSLIYDSDLAVSYTDLDNLFNSDEDELTPGSKKSANGSDDKASCKESKTGNLDPLSCISTADLHKMYPTPPSLEQHIMGFSPMNMNNKEYGSMDTTPGGTVLEGNSSSIGAQFKIEVDEGFCSPKPSEIKDFSYVYKPENCQILVGCSMFAPLKTLPSQYLPPIKLPEECIYRQSWTVGKLELLSSGPSMPFIKEGDGSNMDQEYGTAYTPQTHTSFGMPPSSAPPSNSGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTCRPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYRCTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRATSAEHVNGGLKESEKLSDDLILLLQDQCTNLFSPFGAADQDPFPKSGVISNWVRVEERDCCNDCYLALEHGRQFMDNMSGGKVDEALVKSSCLHPWSKRNDVSMQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPLPIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLGQHRPVSRLLTDGIMRVGSTASKKLSEKLVAEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASLPLDSSLLSQPNLVAPTSQSLITPPQMTNTGNANTPSATLASAASSTMTVTSGVAISTSVATANSTLTTASTSSSSSSNLNSGVSSNKLPSFPPFGSMNSNAAGSMSTQANTVQSGQLGGQQTSALQTAGISGESSSLPTQPHPDVSESTMDRDKVGIPTDGDSHAVTYPPAIVVYIIDPFTYENTDESTNSSSVWTLGLLRCFLEMVQTLPPHIKSTVSVQIIPCQYLLQPVKHEDREIYPQHLKSLAFSAFTQCRRPLPTSTNVKTLTGFGPGLAMETALRSPDRPECIRLYAPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINIDVPNRARRKKSSARKFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDSVSTGSVFGRSTTLNMQTSQLNTPQDTSCTHILVFPTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTGSPVHSPGSHYPHGGDAGKGQSTDRLLSTEPHEEVPNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLHVPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPATQDRRSCLPIHFVVLNQLYNFIMNML

Target ORF information:

RefSeq Version	XM_011525551.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 13 (MED13), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525551.2

ORF Insert Sequence:

ATGAGTGCCT CCTTCGTGCC GAACGGGGCC AGCCTGGAAG ATTGTCACTG TAACCTCTTC 
TGCCTGGCTG ACTTGACAGG AATTAAGTGG AAAAAATATG TATGGCAAGG CCCAACTTCT 
GCCCCTATTC TGTTTCCTGT GACAGAAGAA GACCCCATTT TGAGCAGTTT TAGTCGCTGC 
CTTAAGGCAG ATGTACTTGG TGTTTGGCGG CGAGATCAAA GACCTGGAAG AAGAGAATTG 
TGGATATTTT GGTGGGGTGA AGACCCCAGT TTTGCTGACC TTATTCACCA TGACTTATCA 
GAAGAAGAAG ATGGAGTGTG GGAGAATGGA CTTTCCTATG AATGCCGTAC TCTGCTTTTC 
AAAGCAGTTC ACAATCTATT GGAACGGTGT TTAATGAACA GGAATTTTGT ACGTATTGGC 
AAGTGGTTTG TAAAGCCTTA TGAAAAAGAT GAAAAACCTA TAAATAAAAG TGAACACTTG 
TCCTGCTCCT TCACCTTTTT CTTGCATGGA GACAGCAATG TTTGTACCAG TGTGGAAATT 
AACCAACATC AACCTGTATA CCTTCTCAGT GAAGAGCATA TCACCCTTGC TCAACAGTCT 
AATAGCCCAT TTCAAGTTAT CTTATGCCCA TTTGGACTAA ATGGCACTCT CACAGGACAG 
GCATTCAAGA TGTCTGATTC AGCTACAAAA AAATTAATTG GTGAATGGAA ACAGTTCTAT 
CCTATCTCAT GTTGCTTGAA GGAGATGTCT GAAGAAAAAC AGGAAGATAT GGATTGGGAA 
GATGATTCTT TAGCTGCAGT AGAAGTTCTT GTTGCTGGTG TCCGAATGAT CTACCCAGCA 
TGCTTTGTTC TAGTCCCTCA GTCAGACATT CCTACTCCTA GCCCTGTGGG ATCCACTCAC 
TGTTCATCTT CTTGCTTGGG TGTCCACCAA GTGCCTGCTT CCACAAGAGA TCCTGCTATG 
TCTTCGGTTA CGCTTACACC ACCTACGTCT CCTGAGGAAG TCCAAACAGT TGATCCTCAG 
TCTGTCCAGA AGTGGGTCAA ATTTTCTTCA GTATCTGATG GCTTCAACTC CGATAGTACT 
AGCCACCATG GTGGGAAAAT ACCCAGAAAA TTAGCAAATC ATGTGGTGGA TAGAGTTTGG 
CAAGAATGCA ATATGAACAG AGCACAGAAC AAGAGGAAGT ATTCTGCTTC ATCAGGTGGT 
CTATGCGAAG AAGCGACAGC TGCTAAAGTG GCATCCTGGG ATTTTGTTGA AGCCACACAA 
AGAACAAATT GCAGTTGTTT GAGGCACAAA AATCTCAAGT CAAGAAATGC TGGACAACAA 
GGACAGGCAC CATCTTTAGG TCAGCAACAA CAAATACTTC CTAAGCACAA GACCAATGAG 
AAGCAAGAAA AGAGTGAAAA GCCACAGAAA CGCCCCTTGA CTCCTTTTCA CCATCGTGTG 
TCTGTTAGTG ATGATGTTGG CATGGACGCA GATTCAGCCA GCCAAAGACT TGTGATCTCT 
GCTCCAGACA GTCAAGTGAG ATTTTCAAAT ATCCGAACTA ATGATGTAGC AAAGACTCCT 
CAGATGCATG GCACCGAAAT GGCAAATTCA CCTCAACCAC CCCCACTTAG TCCTCACCCT 
TGTGATGTGG TTGATGAAGG AGTGACTAAA ACACCTTCAA CTCCTCAGAG TCAACATTTT 
TATCAAATGC CAACACCAGA TCCCTTGGTT CCTTCTAAAC CAATGGAAGA TAGGATAGAC 
AGTTTGTCCC AGTCTTTCCC ACCTCAATAT CAGGAAGCTG TAGAACCTAC AGTATATGTT 
GGTACAGCAG TAAACTTGGA AGAAGATGAA GCCAATATAG CCTGGAAGTA TTACAAGTTC 
CCAAAGAAAA AAGATGTAGA GTTTTTACCA CCTCAACTTC CAAGTGATAA ATTCAAGGAT 
GATCCAGTTG GACCTTTTGG ACAGGAAAGT GTAACATCAG TTACAGAGTT AATGGTGCAA 
TGTAAGAAAC CTTTAAAAGT TTCTGATGAA TTAGTGCAGC AATATCAAAT TAAAAACCAG 
TGTCTTTCAG CAATAGCATC TGATGCAGAA CAAGAACCTA AAATTGATCC ATATGCATTT 
GTTGAAGGAG ATGAGGAATT CCTTTTTCCT GATAAAAAAG ATAGACAAAA TAGTGAGAGA 
GAAGCTGGAA AAAAACACAA GGTAGAAGAT GGGACATCTA GTGTAACAGT GTTATCACAT 
GAAGAAGATG CTATGTCATT ATTTAGTCCC TCTATCAAGC AAGATGCTCC ACGCCCTACT 
AGTCATGCCC GTCCTCCATC AACAAGTTTG ATTTATGACT CAGACCTGGC TGTCTCTTAT 
ACTGACCTTG ATAATCTCTT CAATTCTGAT GAAGATGAAC TAACACCTGG ATCTAAAAAA 
TCAGCAAATG GATCAGATGA TAAAGCCAGC TGCAAGGAAT CAAAGACAGG AAATCTGGAC 
CCGTTATCTT GCATAAGCAC TGCAGATCTT CATAAAATGT ATCCTACACC ACCATCATTG 
GAACAACATA TTATGGGATT TTCCCCAATG AATATGAATA ATAAAGAATA TGGTAGTATG 
GATACAACAC CTGGAGGAAC TGTTCTAGAA GGAAATAGTT CTAGTATAGG AGCGCAGTTC 
AAAATTGAGG TTGATGAGGG ATTCTGTAGC CCCAAACCTT CTGAAATTAA AGATTTTTCT 
TATGTCTATA AGCCTGAAAA TTGTCAAATT CTAGTGGGAT GTTCCATGTT TGCACCTCTA 
AAAACTCTAC CAAGCCAATA TCTGCCCCCT ATCAAATTGC CAGAAGAGTG TATTTACCGT 
CAGAGTTGGA CTGTTGGAAA ATTGGAATTG CTTTCTTCAG GGCCTTCAAT GCCATTCATC 
AAAGAGGGTG ATGGAAGTAA TATGGATCAA GAATATGGCA CTGCTTATAC ACCTCAAACT 
CATACTTCTT TTGGGATGCC TCCTAGCAGT GCACCTCCTA GTAACAGCGG AGCAGGAATT 
CTTCCTTCTC CATCCACCCC TCGGTTTCCA ACTCCAAGGA CTCCAAGGAC TCCTCGGACT 
CCTCGTGGAG CTGGTGGACC TGCTAGTGCT CAAGGTTCAG TCAAATATGA AAATTCAGAC 
TTGTATTCAC CAGCTTCTAC CCCATCTACA TGCAGACCCC TTAATTCTGT TGAACCTGCA 
ACTGTCCCTT CCATCCCTGA AGCACACAGT CTTTATGTAA ACCTCATCCT TTCAGAATCA 
GTTATGAATT TGTTTAAAGA CTGTAACTTT GATAGTTGTT GCATCTGTGT TTGCAACATG 
AACATCAAGG GTGCCGATGT TGGAGTTTAC ATTCCAGATC CAACGCAGGA AGCACAATAT 
AGGTGTACCT GTGGCTTCAG TGCTGTCATG AACAGAAAAT TTGGAAACAA TTCAGGATTA 
TTTCTTGAAG ATGAACTAGA TATCATAGGA CGCAATACAG ACTGTGGCAA AGAAGCAGAA 
AAACGTTTTG AAGCTCTCAG GGCTACCTCT GCTGAACATG TTAATGGAGG ACTAAAGGAA 
TCTGAAAAAT TATCTGATGA TTTGATATTA TTGCTACAAG ATCAGTGCAC TAATTTATTT 
TCACCCTTTG GAGCAGCAGA CCAAGATCCT TTTCCTAAAA GTGGTGTAAT TAGCAATTGG 
GTACGTGTTG AAGAGCGTGA CTGTTGCAAT GACTGCTACC TTGCATTAGA ACATGGGCGT 
CAGTTCATGG ATAACATGTC AGGAGGAAAA GTTGATGAAG CACTTGTGAA AAGTTCATGC 
TTACACCCCT GGTCCAAAAG AAACGATGTG AGTATGCAGT GCTCACAGGA TATACTTCGA 
ATGCTCCTCT CTCTTCAGCC AGTTCTTCAG GATGCCATTC AGAAAAAAAG AACAGTAAGA 
CCTTGGGGTG TTCAGGGTCC TCTCACTTGG CAACAATTTC ATAAAATGGC TGGCCGAGGC 
TCTTATGGAA CTGATGAATC CCCAGAACCA CTGCCAATCC CCACATTTTT GTTGGGTTAT 
GATTATGATT ATCTGGTGCT TTCTCCATTT GCTCTTCCTT ATTGGGAGAG ACTTATGCTG 
GAACCCTATG GATCTCAAAG AGATATAGCC TATGTTGTAC TGTGTCCAGA AAATGAAGCC 
TTGTTAAATG GAGCAAAAAG CTTTTTTAGA GATCTTACTG CAATATATGA GTCCTGTCGA 
TTAGGTCAAC ATAGACCTGT TTCTCGACTG TTAACAGATG GGATCATGAG AGTTGGATCT 
ACTGCATCAA AGAAACTATC AGAAAAGTTG GTAGCAGAAT GGTTTTCTCA GGCAGCTGAC 
GGTAACAATG AAGCATTTTC TAAACTCAAG CTTTATGCAC AAGTCTGCAG ATATGACCTA 
GGTCCTTATC TTGCTTCCCT GCCATTGGAC AGCTCTCTAC TTTCCCAGCC AAATTTAGTT 
GCCCCTACAA GTCAGTCTTT GATTACTCCA CCTCAGATGA CAAATACTGG AAATGCTAAT 
ACTCCATCTG CCACCTTAGC ATCTGCAGCG AGCAGCACTA TGACAGTGAC TTCAGGTGTT 
GCCATATCTA CTTCAGTTGC CACAGCTAAT TCAACTTTGA CCACAGCTTC AACTTCATCT 
TCATCATCCT CCAACTTGAA TAGTGGAGTA TCATCAAATA AACTACCTTC GTTTCCACCC 
TTTGGCAGTA TGAACAGTAA TGCTGCAGGA TCCATGTCTA CACAAGCAAA TACAGTTCAG 
AGTGGTCAGC TAGGAGGGCA ACAGACATCA GCTCTACAGA CAGCTGGGAT TTCTGGAGAA 
TCATCTTCAC TTCCCACTCA GCCGCATCCT GATGTGTCTG AAAGCACGAT GGATCGGGAT 
AAAGTGGGAA TCCCCACAGA TGGTGATTCA CATGCAGTCA CGTATCCACC TGCAATTGTT 
GTTTATATAA TTGATCCTTT TACATACGAA AATACAGACG AGAGCACTAA CTCTTCTAGT 
GTGTGGACAT TGGGGCTACT TCGATGCTTT CTAGAAATGG TCCAGACTCT TCCTCCTCAT 
ATCAAGAGTA CTGTTTCTGT ACAGATTATT CCTTGTCAGT ACCTGTTGCA ACCTGTGAAG 
CATGAAGATA GAGAAATCTA TCCCCAGCAT TTAAAATCCC TGGCTTTTTC GGCCTTTACC 
CAGTGTCGGA GGCCACTTCC AACATCAACC AATGTGAAAA CATTGACTGG CTTTGGTCCA 
GGTTTAGCCA TGGAAACTGC CCTTAGAAGT CCTGATAGAC CAGAGTGTAT TCGACTTTAT 
GCACCTCCTT TTATTCTGGC TCCAGTGAAG GACAAACAGA CAGAGCTAGG AGAAACATTT 
GGAGAAGCTG GACAGAAATA TAATGTTCTT TTTGTGGGAT ACTGTTTATC ACATGATCAA 
AGGTGGATTC TTGCATCTTG CACAGATCTA TATGGAGAAC TTTTAGAAAC TTGTATCATT 
AACATCGATG TTCCAAATAG GGCTCGTCGG AAAAAAAGTT CTGCTAGAAA ATTTGGTCTA 
CAGAAACTTT GGGAGTGGTG CTTAGGACTT GTACAAATGA GTTCATTGCC ATGGAGAGTT 
GTAATTGGTC GTCTAGGAAG GATTGGTCAT GGAGAATTGA AAGATTCTGT GTCAACTGGT 
TCTGTATTTG GAAGAAGCAC GACTCTAAAT ATGCAGACAT CTCAGCTAAA TACCCCACAG 
GATACATCAT GTACTCATAT ACTTGTGTTT CCTACTTCTG CTTCTGTGCA AGTAGCTTCA 
GCTACTTATA CCACTGAAAA TTTGGATTTA GCTTTCAATC CCAACAATGA TGGAGCAGAT 
GGAATGGGTA TCTTTGATTT GTTAGACACA GGAGATGATC TTGACCCTGA TATCATTAAT 
ATCCTTCCTG CTTCTCCAAC TGGTTCTCCT GTACATTCTC CAGGATCTCA TTACCCCCAT 
GGAGGTGATG CGGGCAAGGG TCAGAGTACT GATCGGCTAC TATCAACAGA ACCTCATGAG 
GAAGTACCTA ATATTCTTCA GCAACCATTG GCCCTTGGTT ACTTTGTATC AACTGCCAAA 
GCAGGTCCAT TACCTGACTG GTTCTGGTCA GCATGTCCTC AAGCACAATA TCAGTGTCCC 
CTTTTTCTTA AGGCCTCTTT GCACCTCCAC GTGCCTTCAG TGCAATCTGA CGAGCTGCTT 
CACAGTAAAC ACTCCCACCC ACTTGACTCA AATCAGACTT CAGATGTCCT CAGGTTTGTT 
TTGGAACAGT ACAATGCACT CTCCTGGCTA ACCTGTGACC CTGCAACCCA GGACAGACGC 
TCATGTCTCC CAATTCATTT TGTGGTGCTG AATCAGTTAT ATAACTTTAT TATGAATATG 
CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Frameshift Mutations of HSPA4 and MED13 in Gastric and Colorectal Cancers.
Pathology oncology research : POR22(4)769-72(2016 Oct)
Jo YS,Choi MR,Song SY,Kim MS,Yoo NJ,Lee SH

Mediator kinase module and human tumorigenesis.
Critical reviews in biochemistry and molecular biology50(5)393-426(2015)
Clark AD,Oldenbroek M,Boyer TG

MED13-dependent signaling from the heart confers leanness by enhancing metabolism in adipose tissue and liver.
EMBO molecular medicine6(12)1610-21(2014 Dec)
Baskin KK,Grueter CE,Kusminski CM,Holland WL,Bookout AL,Satapati S,Kong YM,Burgess SC,Malloy CR,Scherer PE,Newgard CB,Bassel-Duby R,Olson EN

Genetic determinants of P wave duration and PR segment.
Circulation. Cardiovascular genetics7(4)475-81(2014 Aug)
Verweij N,Mateo Leach I,van den Boogaard M,van Veldhuisen DJ,Christoffels VM,,Hillege HL,van Gilst WH,Barnett P,de Boer RA,van der Harst P

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MED13 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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