CDC27 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC27
Entrez Gene ID	996
Full Name	cell division cycle 27
Synonyms	ANAPC3,APC3,CDC27Hs,D0S1430E,D17S978E,H-NUC,HNUC,NUC2
General protein information	Preferred Names cell division cycle 27 Names cell division cycle protein 27 homolog D0S1430E, D17S978E anaphase promoting complex subunit 3 anaphase-promoting complex, protein 3 cell division cycle 27 homolog nuc2 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.32
Summary	The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y. [provided by RefSeq, May 2014].
Disorder MIM:	116946

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256.4	NP_001247.3	cell division cycle protein 27 homolog isoform 2
XM_017025483.1	XP_016880972.1	cell division cycle protein 27 homolog isoform X6
XM_011525547.2	XP_011523849.1	cell division cycle protein 27 homolog isoform X2
XM_024451052.1	XP_024306820.1	cell division cycle protein 27 homolog isoform X5
NM_001353050.2	NP_001339979.1	cell division cycle protein 27 homolog isoform 8
NM_001353035.2	NP_001339964.1	cell division cycle protein 27 homolog isoform 5
NM_001353047.1	NP_001339976.1	cell division cycle protein 27 homolog isoform 6
XM_011525546.2	XP_011523848.1	cell division cycle protein 27 homolog isoform X1
NM_001293091.2	NP_001280020.1	cell division cycle protein 27 homolog isoform 4
NM_001353050.1	NP_001339979.1	cell division cycle protein 27 homolog isoform 8
XM_017025485.2	XP_016880974.1	cell division cycle protein 27 homolog isoform X7
NM_001353035.1	NP_001339964.1	cell division cycle protein 27 homolog isoform 5
XM_011525548.2	XP_011523850.1	cell division cycle protein 27 homolog isoform X5
XM_017025485.1	XP_016880974.1	cell division cycle protein 27 homolog isoform X9
NM_001293089.1	NP_001280018.1	cell division cycle protein 27 homolog isoform 3
XM_011525550.2	XP_011523852.1	cell division cycle protein 27 homolog isoform X8
NM_001114091.2	NP_001107563.1	cell division cycle protein 27 homolog isoform 1
XM_011525547.3	XP_011523849.1	cell division cycle protein 27 homolog isoform X2
XM_017025481.2	XP_016880970.1	cell division cycle protein 27 homolog isoform X3
XM_011525548.3	XP_011523850.1	cell division cycle protein 27 homolog isoform X4
XM_017025484.2	XP_016880973.1	cell division cycle protein 27 homolog isoform X6
NM_001353049.2	NP_001339978.1	cell division cycle protein 27 homolog isoform 7
XM_017025481.1	XP_016880970.1	cell division cycle protein 27 homolog isoform X3
XM_017025484.1	XP_016880973.1	cell division cycle protein 27 homolog isoform X7
XM_017025482.1	XP_016880971.1	cell division cycle protein 27 homolog isoform X4
NM_001293089.2	NP_001280018.1	cell division cycle protein 27 homolog isoform 3
NM_001353049.1	NP_001339978.1	cell division cycle protein 27 homolog isoform 7
XM_011525546.3	XP_011523848.1	cell division cycle protein 27 homolog isoform X1
XM_017025488.1	XP_016880977.1	cell division cycle protein 27 homolog isoform X9
NM_001353051.2	NP_001339980.1	cell division cycle protein 27 homolog isoform 9
NM_001353047.2	NP_001339976.1	cell division cycle protein 27 homolog isoform 6
NM_001114091.4	NP_001107563.1	cell division cycle protein 27 homolog isoform 1
NM_001114091.3	NP_001107563.1	cell division cycle protein 27 homolog isoform 1
NM_001256.5	NP_001247.3	cell division cycle protein 27 homolog isoform 2
XM_017025487.1	XP_016880976.1	cell division cycle protein 27 homolog isoform X11
NM_001293091.1	NP_001280020.1	cell division cycle protein 27 homolog isoform 4
NM_001353051.1	NP_001339980.1	cell division cycle protein 27 homolog isoform 9
NM_001293091.3	NP_001280020.1	cell division cycle protein 27 homolog isoform 4
NM_001256.6	NP_001247.3	cell division cycle protein 27 homolog isoform 2
NM_001293089.3	NP_001280018.1	cell division cycle protein 27 homolog isoform 3
XM_011525549.2	XP_011523851.1	cell division cycle protein 27 homolog isoform X4
XM_017025486.1	XP_016880975.1	cell division cycle protein 27 homolog isoform X8

Pathways from BioSystems

Homologies

	CDC27	XP_001115976.2
Canis lupus familiaris (dog)	CDC27	XP_548047.2
Rattus norvegicus (Norway rat)	Cdc27	NP_001019964.1
Homo sapiens (human)	CDC27	NP_001107563.1
Bos taurus (cattle)	CDC27	NP_001098898.1
Gallus gallus (chicken)	CDC27	NP_001006331.1
Danio rerio (zebrafish)	cdc27	NP_958857.1
Mus musculus (house mouse)	Cdc27	NP_663411.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007690	XP_308181.4
Oryza sativa (rice)	Os06g0622500	NP_001058099.2
Xenopus tropicalis (tropical clawed frog)	cdc27	NP_001072470.1
Pan troglodytes (chimpanzee)	CDC27	XP_511624.2
Drosophila melanogaster (fruit fly)	Cdc27	NP_001261503.1
Arabidopsis thaliana (thale cress)	HBT	NP_849994.1

Gene Ontology
Bibliography

Related articles in PubMed

Inhibition of Anaphase-Promoting Complex by Silence APC/C
Wang C, Su Z, Hou H, Li D, Pan Z, Tian W, Mo C
Journal of cellular biochemistry118(10)3150-3157(2017 Oct)

UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.
Yoshimura S, Kasamatsu A, Nakashima D, Iyoda M, Kasama H, Saito T, Takahara T, Endo-Sakamoto Y, Shiiba M, Tanzawa H, Uzawa K
Biochemical and biophysical research communications485(4)820-825(2017 04)

mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27.
Feng Z, Zhang L, Zhou J, Zhou S, Li L, Guo X, Feng G, Ma Z, Huang W, Huang F
Oncotarget8(4)6304-6318(2017 Jan)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The levels of CDC20 and CylinB1 increased and the levels of Ku70 and APC3 decreased after irradiation. APC/C(Cdh1) is involved in regulation of radiosensitivity in human NPC CNE-1 cells.
Title: Inhibition of Anaphase-Promoting Complex by Silence APC/C

Data indicate that UBE2S and APC3 co-regulate the expression level of P21 at G2/M check point via the ubiquitin-proteasome system.
Title: UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.

Cdc27 is a novel binding partner of Elmo1.Cdc27-Elmo1 has a cellular role independent from the Elmo-Dock1-Rac signal module.
Title: Identification of a novel protein interaction between Elmo1 and Cdc27.

These findings suggested that polymorphisms in cdc27 may contribute to the susceptibility of BC though functional studies are needed to further elucidate the underling mechanisms of the associations
Title: Association between polymorphisms in cdc27 and breast cancer in a Chinese population.

miR-27a could modulate proliferation and radiosensitivity of TNBC cells. CDC-27 is a direct target of miR-27a and its downregulation conferred increased radioresistance of the cells.
Title: MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27.

The following CDC27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27728	NM_001114091.4 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$419.50-$587.30 $839.00
OHu87732	XM_017025488.1 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu102845	NM_001353051.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu56775	XM_011525547.3 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu87726	NM_001353035.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87727	NM_001353047.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87731	NM_001353049.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87725	XM_017025481.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87726	XM_017025482.1 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87727	XM_017025483.1 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87731	XM_017025487.1 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu27729	NM_001256.6 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27730	NM_001293089.3 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27731	NM_001293091.3 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56777	NM_001353050.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27731	XM_024451052.1 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56774	XM_011525546.3 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56776	XM_011525548.3 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56776	XM_011525549.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56777	XM_011525550.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87728	XM_017025484.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87729	XM_017025485.2 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87730	XM_017025486.1 Latest version!	Homo sapiens cell division cycle 27 (CDC27), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu56777	NM_001353050.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87726	NM_001353035.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87729	XM_017025485.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu56775	XM_011525547.2	Homo sapiens cell division cycle 27 (CDC27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu56776	XM_011525548.2	Homo sapiens cell division cycle 27 (CDC27), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87725	XM_017025481.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu87728	XM_017025484.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27730	NM_001293089.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87731	NM_001353049.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu56774	XM_011525546.2	Homo sapiens cell division cycle 27 (CDC27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu87727	NM_001353047.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu27728	NM_001114091.2	Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$419.50-$587.30 $839.00
OHu27728	NM_001114091.3	Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$419.50-$587.30 $839.00
OHu27729	NM_001256.4	Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27731	NM_001293091.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu102845	NM_001353051.1	Homo sapiens cell division cycle 27 (CDC27), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu27731	NM_001293091.2	Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27729	NM_001256.5	Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu27730	NM_001293089.2	Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC27 ( NM_001114091.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107563.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27728
Clone ID Related Accession (Same CDS sequence)	NM_001114091.2 , NM_001114091.4 , NM_001114091.3
Accession Version	NM_001114091.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, BC011656.2, AC002558.2 and AA927469.1. On May 28, 2014 this sequence version replaced NM_001114091.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011656.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGGTTTACA AGAAGTTAGG TCAAACGCAC 
CTCGCCCTGA TGAATTTCTC TTGGGCTATG GATTTAGATC CTAAAGGAGC CAATAACCAG 
ATTAAAGAGG CAATTGATAA GCGTTATCTT CCAGATGATG AGGAGCCAAT AACCCAAGAA 
GAACAGATCA TGGGAACAGA TGAATCCCAG GAGAGCAGCA TGACAGATGC GGATGACACA 
CAACTTCATG CAGCTGAAAG TGATGAATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107563.1
CDS	141..2633
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001114091.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114091.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGGTTTACA AGAAGTTAGG TCAAACGCAC 
CTCGCCCTGA TGAATTTCTC TTGGGCTATG GATTTAGATC CTAAAGGAGC CAATAACCAG 
ATTAAAGAGG CAATTGATAA GCGTTATCTT CCAGATGATG AGGAGCCAAT AACCCAAGAA 
GAACAGATCA TGGGAACAGA TGAATCCCAG GAGAGCAGCA TGACAGATGC GGATGACACA 
CAACTTCATG CAGCTGAAAG TGATGAATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001114091.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107563.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27728
Clone ID Related Accession (Same CDS sequence)	NM_001114091.2 , NM_001114091.4 , NM_001114091.3
Accession Version	NM_001114091.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, BC011656.2, AC002558.2 and AA927469.1. On May 31, 2019 this sequence version replaced NM_001114091.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011656.2, SRR1660809.244186.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGGTTTACA AGAAGTTAGG TCAAACGCAC 
CTCGCCCTGA TGAATTTCTC TTGGGCTATG GATTTAGATC CTAAAGGAGC CAATAACCAG 
ATTAAAGAGG CAATTGATAA GCGTTATCTT CCAGATGATG AGGAGCCAAT AACCCAAGAA 
GAACAGATCA TGGGAACAGA TGAATCCCAG GAGAGCAGCA TGACAGATGC GGATGACACA 
CAACTTCATG CAGCTGAAAG TGATGAATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107563.1
CDS	124..2616
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001114091.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114091.4

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGGTTTACA AGAAGTTAGG TCAAACGCAC 
CTCGCCCTGA TGAATTTCTC TTGGGCTATG GATTTAGATC CTAAAGGAGC CAATAACCAG 
ATTAAAGAGG CAATTGATAA GCGTTATCTT CCAGATGATG AGGAGCCAAT AACCCAAGAA 
GAACAGATCA TGGGAACAGA TGAATCCCAG GAGAGCAGCA TGACAGATGC GGATGACACA 
CAACTTCATG CAGCTGAAAG TGATGAATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001114091.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001107563.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27728
Clone ID Related Accession (Same CDS sequence)	NM_001114091.2 , NM_001114091.4 , NM_001114091.3
Accession Version	NM_001114091.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2, HY023336.1, BC011656.2 and AA927469.1. On Jun 28, 2017 this sequence version replaced NM_001114091.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.244186.1, BC011656.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGGTTTACA AGAAGTTAGG TCAAACGCAC 
CTCGCCCTGA TGAATTTCTC TTGGGCTATG GATTTAGATC CTAAAGGAGC CAATAACCAG 
ATTAAAGAGG CAATTGATAA GCGTTATCTT CCAGATGATG AGGAGCCAAT AACCCAAGAA 
GAACAGATCA TGGGAACAGA TGAATCCCAG GAGAGCAGCA TGACAGATGC GGATGACACA 
CAACTTCATG CAGCTGAAAG TGATGAATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001107563.1
CDS	168..2660
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001114091.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114091.3

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGGTTTACA AGAAGTTAGG TCAAACGCAC 
CTCGCCCTGA TGAATTTCTC TTGGGCTATG GATTTAGATC CTAAAGGAGC CAATAACCAG 
ATTAAAGAGG CAATTGATAA GCGTTATCTT CCAGATGATG AGGAGCCAAT AACCCAAGAA 
GAACAGATCA TGGGAACAGA TGAATCCCAG GAGAGCAGCA TGACAGATGC GGATGACACA 
CAACTTCATG CAGCTGAAAG TGATGAATTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025488.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880977.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu87732
Clone ID Related Accession (Same CDS sequence)	XM_017025488.1
Accession Version	XM_017025488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880977.1
CDS	1299..2582
Translation	MKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_017025488.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025488.1

ORF Insert Sequence:

ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353051.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339980.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu102845
Clone ID Related Accession (Same CDS sequence)	NM_001353051.2 , NM_001353051.1
Accession Version	NM_001353051.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On May 31, 2019 this sequence version replaced NM_001353051.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.169886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGGTT TGATGAGCCT TCTTCGTGAA ATGGGGAAAG GTTATTTAGC TTTGTGTTCA 
TACAACTGCA AAGAAGCTAT AAATATTTTG AGCCATCTAC CTTCTCACCA CTACAATACT 
GGTTGGGTAC TGTGCCAAAT TGGAAGGGCC TATTTTGAAC TTTCAGAGTA CATGCAAGCT 
GAAAGAATAT TCTCAGAGGT TAGAAGGATT GAGAATTATA GAGTTGAAGG CATGGAGATC 
TACTCTACAA CACTTTGGCA TCTTCAAAAA GATGTTGCTC TTTCAGTTCT GTCAAAAGAC 
TTAACAGACA TGGATAAAAA TTCGCCAGAG GCCTGGTGTG CTGCAGGGAA CTGTTTCAGT 
CTGCAACGGG AACATGATAT TGCAATTAAA TTCTTCCAGA GAGCTATCCA AGTTGATCCA 
AATTACGCTT ATGCCTATAC TCTATTAGGG CATGAGTTTG TCTTAACTGA AGAATTGGAC 
AAAGCATTAG CTTGTTTTCG AAATGCTATC AGAGTCAATC CTAGACATTA TAATGCATGG 
TATGGTTTAG GAATGATTTA TTACAAGCAA GAAAAATTCA GCCTTGCAGA AATGCATTTC 
CAAAAAGCGC TTGATATCAA CCCTCAAAGT TCAGTTTTAC TTTGCCACAT TGGAGTAGTT 
CAACATGCAC TGAAAAAATC AGAGAAGGCT TTGGATACCC TAAACAAAGC CATTGTCATT 
GATCCCAAGA ACCCTCTATG CAAATTTCAC AGAGCCTCAG TTTTATTTGC AAATGAAAAA 
TATAAGTCTG CTTTACAAGA ACTTGAAGAA TTGAAACAAA TTGTTCCCAA AGAATCCCTC 
GTTTACTTCT TAATAGGAAA GGTTTACAAG AAGTTAGGTC AAACGCACCT CGCCCTGATG 
AATTTCTCTT GGGCTATGGA TTTAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA 
ATTGATAAGC GTTATCTTCC AGATGATGAG GAGCCAATAA CCCAAGAAGA ACAGATCATG 
GGAACAGATG AATCCCAGGA GAGCAGCATG ACAGATGCGG ATGACACACA ACTTCATGCA 
GCTGAAAGTG ATGAATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339980.1
CDS	1119..2399
Translation	MKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001353051.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353051.2

ORF Insert Sequence:

ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGGTT TGATGAGCCT TCTTCGTGAA ATGGGGAAAG GTTATTTAGC TTTGTGTTCA 
TACAACTGCA AAGAAGCTAT AAATATTTTG AGCCATCTAC CTTCTCACCA CTACAATACT 
GGTTGGGTAC TGTGCCAAAT TGGAAGGGCC TATTTTGAAC TTTCAGAGTA CATGCAAGCT 
GAAAGAATAT TCTCAGAGGT TAGAAGGATT GAGAATTATA GAGTTGAAGG CATGGAGATC 
TACTCTACAA CACTTTGGCA TCTTCAAAAA GATGTTGCTC TTTCAGTTCT GTCAAAAGAC 
TTAACAGACA TGGATAAAAA TTCGCCAGAG GCCTGGTGTG CTGCAGGGAA CTGTTTCAGT 
CTGCAACGGG AACATGATAT TGCAATTAAA TTCTTCCAGA GAGCTATCCA AGTTGATCCA 
AATTACGCTT ATGCCTATAC TCTATTAGGG CATGAGTTTG TCTTAACTGA AGAATTGGAC 
AAAGCATTAG CTTGTTTTCG AAATGCTATC AGAGTCAATC CTAGACATTA TAATGCATGG 
TATGGTTTAG GAATGATTTA TTACAAGCAA GAAAAATTCA GCCTTGCAGA AATGCATTTC 
CAAAAAGCGC TTGATATCAA CCCTCAAAGT TCAGTTTTAC TTTGCCACAT TGGAGTAGTT 
CAACATGCAC TGAAAAAATC AGAGAAGGCT TTGGATACCC TAAACAAAGC CATTGTCATT 
GATCCCAAGA ACCCTCTATG CAAATTTCAC AGAGCCTCAG TTTTATTTGC AAATGAAAAA 
TATAAGTCTG CTTTACAAGA ACTTGAAGAA TTGAAACAAA TTGTTCCCAA AGAATCCCTC 
GTTTACTTCT TAATAGGAAA GGTTTACAAG AAGTTAGGTC AAACGCACCT CGCCCTGATG 
AATTTCTCTT GGGCTATGGA TTTAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA 
ATTGATAAGC GTTATCTTCC AGATGATGAG GAGCCAATAA CCCAAGAAGA ACAGATCATG 
GGAACAGATG AATCCCAGGA GAGCAGCATG ACAGATGCGG ATGACACACA ACTTCATGCA 
GCTGAAAGTG ATGAATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353051.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339980.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu102845
Clone ID Related Accession (Same CDS sequence)	NM_001353051.2 , NM_001353051.1
Accession Version	NM_001353051.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-16
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.169886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGGTT TGATGAGCCT TCTTCGTGAA ATGGGGAAAG GTTATTTAGC TTTGTGTTCA 
TACAACTGCA AAGAAGCTAT AAATATTTTG AGCCATCTAC CTTCTCACCA CTACAATACT 
GGTTGGGTAC TGTGCCAAAT TGGAAGGGCC TATTTTGAAC TTTCAGAGTA CATGCAAGCT 
GAAAGAATAT TCTCAGAGGT TAGAAGGATT GAGAATTATA GAGTTGAAGG CATGGAGATC 
TACTCTACAA CACTTTGGCA TCTTCAAAAA GATGTTGCTC TTTCAGTTCT GTCAAAAGAC 
TTAACAGACA TGGATAAAAA TTCGCCAGAG GCCTGGTGTG CTGCAGGGAA CTGTTTCAGT 
CTGCAACGGG AACATGATAT TGCAATTAAA TTCTTCCAGA GAGCTATCCA AGTTGATCCA 
AATTACGCTT ATGCCTATAC TCTATTAGGG CATGAGTTTG TCTTAACTGA AGAATTGGAC 
AAAGCATTAG CTTGTTTTCG AAATGCTATC AGAGTCAATC CTAGACATTA TAATGCATGG 
TATGGTTTAG GAATGATTTA TTACAAGCAA GAAAAATTCA GCCTTGCAGA AATGCATTTC 
CAAAAAGCGC TTGATATCAA CCCTCAAAGT TCAGTTTTAC TTTGCCACAT TGGAGTAGTT 
CAACATGCAC TGAAAAAATC AGAGAAGGCT TTGGATACCC TAAACAAAGC CATTGTCATT 
GATCCCAAGA ACCCTCTATG CAAATTTCAC AGAGCCTCAG TTTTATTTGC AAATGAAAAA 
TATAAGTCTG CTTTACAAGA ACTTGAAGAA TTGAAACAAA TTGTTCCCAA AGAATCCCTC 
GTTTACTTCT TAATAGGAAA GGTTTACAAG AAGTTAGGTC AAACGCACCT CGCCCTGATG 
AATTTCTCTT GGGCTATGGA TTTAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA 
ATTGATAAGC GTTATCTTCC AGATGATGAG GAGCCAATAA CCCAAGAAGA ACAGATCATG 
GGAACAGATG AATCCCAGGA GAGCAGCATG ACAGATGCGG ATGACACACA ACTTCATGCA 
GCTGAAAGTG ATGAATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339980.1
CDS	1163..2443
Translation	MKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001353051.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353051.1

ORF Insert Sequence:

ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGGTT TGATGAGCCT TCTTCGTGAA ATGGGGAAAG GTTATTTAGC TTTGTGTTCA 
TACAACTGCA AAGAAGCTAT AAATATTTTG AGCCATCTAC CTTCTCACCA CTACAATACT 
GGTTGGGTAC TGTGCCAAAT TGGAAGGGCC TATTTTGAAC TTTCAGAGTA CATGCAAGCT 
GAAAGAATAT TCTCAGAGGT TAGAAGGATT GAGAATTATA GAGTTGAAGG CATGGAGATC 
TACTCTACAA CACTTTGGCA TCTTCAAAAA GATGTTGCTC TTTCAGTTCT GTCAAAAGAC 
TTAACAGACA TGGATAAAAA TTCGCCAGAG GCCTGGTGTG CTGCAGGGAA CTGTTTCAGT 
CTGCAACGGG AACATGATAT TGCAATTAAA TTCTTCCAGA GAGCTATCCA AGTTGATCCA 
AATTACGCTT ATGCCTATAC TCTATTAGGG CATGAGTTTG TCTTAACTGA AGAATTGGAC 
AAAGCATTAG CTTGTTTTCG AAATGCTATC AGAGTCAATC CTAGACATTA TAATGCATGG 
TATGGTTTAG GAATGATTTA TTACAAGCAA GAAAAATTCA GCCTTGCAGA AATGCATTTC 
CAAAAAGCGC TTGATATCAA CCCTCAAAGT TCAGTTTTAC TTTGCCACAT TGGAGTAGTT 
CAACATGCAC TGAAAAAATC AGAGAAGGCT TTGGATACCC TAAACAAAGC CATTGTCATT 
GATCCCAAGA ACCCTCTATG CAAATTTCAC AGAGCCTCAG TTTTATTTGC AAATGAAAAA 
TATAAGTCTG CTTTACAAGA ACTTGAAGAA TTGAAACAAA TTGTTCCCAA AGAATCCCTC 
GTTTACTTCT TAATAGGAAA GGTTTACAAG AAGTTAGGTC AAACGCACCT CGCCCTGATG 
AATTTCTCTT GGGCTATGGA TTTAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA 
ATTGATAAGC GTTATCTTCC AGATGATGAG GAGCCAATAA CCCAAGAAGA ACAGATCATG 
GGAACAGATG AATCCCAGGA GAGCAGCATG ACAGATGCGG ATGACACACA ACTTCATGCA 
GCTGAAAGTG ATGAATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525547.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523849.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu56775
Clone ID Related Accession (Same CDS sequence)	XM_011525547.2 , XM_011525547.3
Accession Version	XM_011525547.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGATCCTAA AGGAGCCAAT AACCAGATTA 
AAGAGGCAAT TGATAAGCGT TATCTTCCAG ATGATGAGGA GCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523849.1
CDS	128..2455
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_011525547.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525547.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGATCCTAA AGGAGCCAAT AACCAGATTA 
AAGAGGCAAT TGATAAGCGT TATCTTCCAG ATGATGAGGA GCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525547.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523849.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X3, mRNA.

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CloneID	OHu56775
Clone ID Related Accession (Same CDS sequence)	XM_011525547.2 , XM_011525547.3
Accession Version	XM_011525547.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525547.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGATCCTAA AGGAGCCAAT AACCAGATTA 
AAGAGGCAAT TGATAAGCGT TATCTTCCAG ATGATGAGGA GCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523849.1
CDS	102..2429
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_011525547.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525547.3

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGAAGG TTTGATGAGC CTTCTTCGTG AAATGGGGAA AGGTTATTTA 
GCTTTGTGTT CATACAACTG CAAAGAAGCT ATAAATATTT TGAGCCATCT ACCTTCTCAC 
CACTACAATA CTGGTTGGGT ACTGTGCCAA ATTGGAAGGG CCTATTTTGA ACTTTCAGAG 
TACATGCAAG CTGAAAGAAT ATTCTCAGAG GTTAGAAGGA TTGAGAATTA TAGAGTTGAA 
GGCATGGAGA TCTACTCTAC AACACTTTGG CATCTTCAAA AAGATGTTGC TCTTTCAGTT 
CTGTCAAAAG ACTTAACAGA CATGGATAAA AATTCGCCAG AGGCCTGGTG TGCTGCAGGG 
AACTGTTTCA GTCTGCAACG GGAACATGAT ATTGCAATTA AATTCTTCCA GAGAGCTATC 
CAAGTTGATC CAAATTACGC TTATGCCTAT ACTCTATTAG GGCATGAGTT TGTCTTAACT 
GAAGAATTGG ACAAAGCATT AGCTTGTTTT CGAAATGCTA TCAGAGTCAA TCCTAGACAT 
TATAATGCAT GGTATGGTTT AGGAATGATT TATTACAAGC AAGAAAAATT CAGCCTTGCA 
GAAATGCATT TCCAAAAAGC GCTTGATATC AACCCTCAAA GTTCAGTTTT ACTTTGCCAC 
ATTGGAGTAG TTCAACATGC ACTGAAAAAA TCAGAGAAGG CTTTGGATAC CCTAAACAAA 
GCCATTGTCA TTGATCCCAA GAACCCTCTA TGCAAATTTC ACAGAGCCTC AGTTTTATTT 
GCAAATGAAA AATATAAGTC TGCTTTACAA GAACTTGAAG AATTGAAACA AATTGTTCCC 
AAAGAATCCC TCGTTTACTT CTTAATAGGA AAGATCCTAA AGGAGCCAAT AACCAGATTA 
AAGAGGCAAT TGATAAGCGT TATCTTCCAG ATGATGAGGA GCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353035.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339964.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 5, mRNA.

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CloneID	OHu87726
Clone ID Related Accession (Same CDS sequence)	NM_001353035.2 , NM_001353035.1 , XM_017025482.1
Accession Version	NM_001353035.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On May 31, 2019 this sequence version replaced NM_001353035.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.17650.1, SRR1803614.269236.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC 
GTTATCTTCC AGATGATGAG GAGCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339964.1
CDS	124..2433
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	NM_001353035.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353035.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC 
GTTATCTTCC AGATGATGAG GAGCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353035.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339964.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu87726
Clone ID Related Accession (Same CDS sequence)	NM_001353035.2 , NM_001353035.1 , XM_017025482.1
Accession Version	NM_001353035.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On Jun 28, 2017 this sequence version replaced XM_017025482.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.17650.1, SRR1803614.269236.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC 
GTTATCTTCC AGATGATGAG GAGCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339964.1
CDS	168..2477
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	NM_001353035.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353035.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC 
GTTATCTTCC AGATGATGAG GAGCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353047.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339976.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu87727
Clone ID Related Accession (Same CDS sequence)	XM_017025483.1 , NM_001353047.1 , NM_001353047.2
Accession Version	NM_001353047.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On Jun 28, 2017 this sequence version replaced XM_017025483.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.205587.1, SRR1660805.110354.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339976.1
CDS	168..2474
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	NM_001353047.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353047.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353047.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339976.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu87727
Clone ID Related Accession (Same CDS sequence)	XM_017025483.1 , NM_001353047.1 , NM_001353047.2
Accession Version	NM_001353047.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On May 31, 2019 this sequence version replaced NM_001353047.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.205587.1, SRR1660807.61673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339976.1
CDS	124..2430
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	NM_001353047.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353047.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353049.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339978.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu87731
Clone ID Related Accession (Same CDS sequence)	NM_001353049.2 , NM_001353049.1 , XM_017025487.1
Accession Version	NM_001353049.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On May 31, 2019 this sequence version replaced NM_001353049.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.241438.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339978.1
CDS	159..2285
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	NM_001353049.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353049.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353049.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339978.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 7, mRNA.

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CloneID	OHu87731
Clone ID Related Accession (Same CDS sequence)	NM_001353049.2 , NM_001353049.1 , XM_017025487.1
Accession Version	NM_001353049.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On Jun 28, 2017 this sequence version replaced XM_017025487.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.241438.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339978.1
CDS	203..2329
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	NM_001353049.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353049.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025481.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880970.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X4, mRNA.

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CloneID	OHu87725
Clone ID Related Accession (Same CDS sequence)	XM_017025481.2 , XM_017025481.1
Accession Version	XM_017025481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG 
AGGCAATTGA TAAGCGTTAT CTTCCAGATG ATGAGGAGCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880970.1
CDS	102..2426
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_017025481.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025481.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG 
AGGCAATTGA TAAGCGTTAT CTTCCAGATG ATGAGGAGCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025481.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880970.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu87725
Clone ID Related Accession (Same CDS sequence)	XM_017025481.2 , XM_017025481.1
Accession Version	XM_017025481.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG 
AGGCAATTGA TAAGCGTTAT CTTCCAGATG ATGAGGAGCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880970.1
CDS	128..2452
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_017025481.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025481.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG 
AGGCAATTGA TAAGCGTTAT CTTCCAGATG ATGAGGAGCC AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025482.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880971.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu87726
Clone ID Related Accession (Same CDS sequence)	NM_001353035.2 , NM_001353035.1 , XM_017025482.1
Accession Version	XM_017025482.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC 
GTTATCTTCC AGATGATGAG GAGCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880971.1
CDS	128..2437
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_017025482.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025482.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGATCCT AAAGGAGCCA ATAACCAGAT TAAAGAGGCA ATTGATAAGC 
GTTATCTTCC AGATGATGAG GAGCCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025483.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880972.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu87727
Clone ID Related Accession (Same CDS sequence)	XM_017025483.1 , NM_001353047.1 , NM_001353047.2
Accession Version	XM_017025483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880972.1
CDS	128..2434
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_017025483.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025483.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025487.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880976.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X13, mRNA.

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CloneID	OHu87731
Clone ID Related Accession (Same CDS sequence)	NM_001353049.2 , NM_001353049.1 , XM_017025487.1
Accession Version	XM_017025487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880976.1
CDS	195..2321
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKILKEPITRLKRQLISVIFQMMRSQ

Target ORF information:

RefSeq Version	XM_017025487.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025487.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGATCCTAAA GGAGCCAATA ACCAGATTAA AGAGGCAATT GATAAGCGTT 
ATCTTCCAGA TGATGAGGAG CCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001256.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247.3 Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.

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CloneID	OHu27729
Clone ID Related Accession (Same CDS sequence)	NM_001256.4 , NM_001256.5 , NM_001256.6
Accession Version	NM_001256.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform 2
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, S78234.1, AC002558.2 and AA927469.1. On May 28, 2014 this sequence version replaced NM_001256.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S78234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGGTTTA CAAGAAGTTA GGTCAAACGC ACCTCGCCCT GATGAATTTC 
TCTTGGGCTA TGGATTTAGA TCCTAAAGGA GCCAATAACC AGATTAAAGA GGCAATTGAT 
AAGCGTTATC TTCCAGATGA TGAGGAGCCA ATAACCCAAG AAGAACAGAT CATGGGAACA 
GATGAATCCC AGGAGAGCAG CATGACAGAT GCGGATGACA CACAACTTCA TGCAGCTGAA 
AGTGATGAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247.3
CDS	141..2615
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001256.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256.4

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGGTTTA CAAGAAGTTA GGTCAAACGC ACCTCGCCCT GATGAATTTC 
TCTTGGGCTA TGGATTTAGA TCCTAAAGGA GCCAATAACC AGATTAAAGA GGCAATTGAT 
AAGCGTTATC TTCCAGATGA TGAGGAGCCA ATAACCCAAG AAGAACAGAT CATGGGAACA 
GATGAATCCC AGGAGAGCAG CATGACAGAT GCGGATGACA CACAACTTCA TGCAGCTGAA 
AGTGATGAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001256.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247.3 Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27729
Clone ID Related Accession (Same CDS sequence)	NM_001256.4 , NM_001256.5 , NM_001256.6
Accession Version	NM_001256.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2, HY023336.1, S78234.1 and AA927469.1. On Jun 28, 2017 this sequence version replaced NM_001256.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.224122.1, SRR1803615.70207.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGGTTTA CAAGAAGTTA GGTCAAACGC ACCTCGCCCT GATGAATTTC 
TCTTGGGCTA TGGATTTAGA TCCTAAAGGA GCCAATAACC AGATTAAAGA GGCAATTGAT 
AAGCGTTATC TTCCAGATGA TGAGGAGCCA ATAACCCAAG AAGAACAGAT CATGGGAACA 
GATGAATCCC AGGAGAGCAG CATGACAGAT GCGGATGACA CACAACTTCA TGCAGCTGAA 
AGTGATGAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247.3
CDS	168..2642
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001256.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256.5

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGGTTTA CAAGAAGTTA GGTCAAACGC ACCTCGCCCT GATGAATTTC 
TCTTGGGCTA TGGATTTAGA TCCTAAAGGA GCCAATAACC AGATTAAAGA GGCAATTGAT 
AAGCGTTATC TTCCAGATGA TGAGGAGCCA ATAACCCAAG AAGAACAGAT CATGGGAACA 
GATGAATCCC AGGAGAGCAG CATGACAGAT GCGGATGACA CACAACTTCA TGCAGCTGAA 
AGTGATGAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001256.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247.3 Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.

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CloneID	OHu27729
Clone ID Related Accession (Same CDS sequence)	NM_001256.4 , NM_001256.5 , NM_001256.6
Accession Version	NM_001256.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, S78234.1, AC002558.2 and AA927469.1. On Nov 23, 2018 this sequence version replaced NM_001256.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.224122.1, SRR1803615.70207.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000066544.8/ ENSP00000066544.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGGTTTA CAAGAAGTTA GGTCAAACGC ACCTCGCCCT GATGAATTTC 
TCTTGGGCTA TGGATTTAGA TCCTAAAGGA GCCAATAACC AGATTAAAGA GGCAATTGAT 
AAGCGTTATC TTCCAGATGA TGAGGAGCCA ATAACCCAAG AAGAACAGAT CATGGGAACA 
GATGAATCCC AGGAGAGCAG CATGACAGAT GCGGATGACA CACAACTTCA TGCAGCTGAA 
AGTGATGAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247.3
CDS	124..2598
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001256.6
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256.6

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGAA 
GGTTTGATGA GCCTTCTTCG TGAAATGGGG AAAGGTTATT TAGCTTTGTG TTCATACAAC 
TGCAAAGAAG CTATAAATAT TTTGAGCCAT CTACCTTCTC ACCACTACAA TACTGGTTGG 
GTACTGTGCC AAATTGGAAG GGCCTATTTT GAACTTTCAG AGTACATGCA AGCTGAAAGA 
ATATTCTCAG AGGTTAGAAG GATTGAGAAT TATAGAGTTG AAGGCATGGA GATCTACTCT 
ACAACACTTT GGCATCTTCA AAAAGATGTT GCTCTTTCAG TTCTGTCAAA AGACTTAACA 
GACATGGATA AAAATTCGCC AGAGGCCTGG TGTGCTGCAG GGAACTGTTT CAGTCTGCAA 
CGGGAACATG ATATTGCAAT TAAATTCTTC CAGAGAGCTA TCCAAGTTGA TCCAAATTAC 
GCTTATGCCT ATACTCTATT AGGGCATGAG TTTGTCTTAA CTGAAGAATT GGACAAAGCA 
TTAGCTTGTT TTCGAAATGC TATCAGAGTC AATCCTAGAC ATTATAATGC ATGGTATGGT 
TTAGGAATGA TTTATTACAA GCAAGAAAAA TTCAGCCTTG CAGAAATGCA TTTCCAAAAA 
GCGCTTGATA TCAACCCTCA AAGTTCAGTT TTACTTTGCC ACATTGGAGT AGTTCAACAT 
GCACTGAAAA AATCAGAGAA GGCTTTGGAT ACCCTAAACA AAGCCATTGT CATTGATCCC 
AAGAACCCTC TATGCAAATT TCACAGAGCC TCAGTTTTAT TTGCAAATGA AAAATATAAG 
TCTGCTTTAC AAGAACTTGA AGAATTGAAA CAAATTGTTC CCAAAGAATC CCTCGTTTAC 
TTCTTAATAG GAAAGGTTTA CAAGAAGTTA GGTCAAACGC ACCTCGCCCT GATGAATTTC 
TCTTGGGCTA TGGATTTAGA TCCTAAAGGA GCCAATAACC AGATTAAAGA GGCAATTGAT 
AAGCGTTATC TTCCAGATGA TGAGGAGCCA ATAACCCAAG AAGAACAGAT CATGGGAACA 
GATGAATCCC AGGAGAGCAG CATGACAGAT GCGGATGACA CACAACTTCA TGCAGCTGAA 
AGTGATGAAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001293089.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280018.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27730
Clone ID Related Accession (Same CDS sequence)	NM_001293089.1 , NM_001293089.2 , NM_001293089.3
Accession Version	NM_001293089.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform 3
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, U00001.1, AC002558.2 and AA927469.1. On May 28, 2014 this sequence version replaced XM_005257892.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U00001.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280018.1
CDS	141..2612
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001293089.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293089.1

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001293089.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280018.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27730
Clone ID Related Accession (Same CDS sequence)	NM_001293089.1 , NM_001293089.2 , NM_001293089.3
Accession Version	NM_001293089.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2, HY023336.1, U00001.1 and AA927469.1. On Jun 28, 2017 this sequence version replaced NM_001293089.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.245793.1, SRR1803617.91054.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280018.1
CDS	168..2639
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001293089.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293089.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001293089.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280018.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27730
Clone ID Related Accession (Same CDS sequence)	NM_001293089.1 , NM_001293089.2 , NM_001293089.3
Accession Version	NM_001293089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, U00001.1, AC002558.2 and AA927469.1. On May 31, 2019 this sequence version replaced NM_001293089.2. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.245793.1, SRR1660807.141675.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280018.1
CDS	124..2595
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001293089.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293089.3

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGTCT 
GTTGCCAGAA TCGGCCAAAC TGGAACAAAG TCTGTCTTCT CACAGAGTGG AAATAGCCGA 
GAGGTAACTC CAATTCTTGC ACAAACACAA AGTTCTGGTC CACAAACAAG TACAACACCT 
CAGGTATTGA GCCCCACTAT TACATCTCCC CCAAACGCAC TGCCTCGAAG AAGTTCACGA 
CTCTTTACTA GTGACAGCTC CACAACCAAG GAGAATAGCA AAAAATTAAA AATGAAGTTT 
CCACCTAAAA TCCCAAACAG AAAAACAAAA AGTAAAACTA ATAAAGGAGG AATAACTCAA 
CCTAACATAA ATGATAGCCT GGAAATTACA AAATTGGACT CTTCCATCAT TTCAGAAGGG 
AAAATATCCA CAATCACACC TCAGATTCAG GCCTTTAATC TACAAAAAGC AGCAGCAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001293091.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280020.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27731
Clone ID Related Accession (Same CDS sequence)	XM_024451052.1 , NM_001293091.2 , NM_001293091.1 , NM_001293091.3
Accession Version	NM_001293091.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2, HY023336.1, AK296881.1 and AA927469.1. On Jun 28, 2017 this sequence version replaced NM_001293091.1. Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and it thus differs in its 5' UTR and initiates translation at an alternate start codon, and it also uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.224081.1, SRR1660805.109066.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280020.1
CDS	203..2494
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001293091.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293091.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001293091.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280020.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27731
Clone ID Related Accession (Same CDS sequence)	XM_024451052.1 , NM_001293091.2 , NM_001293091.1 , NM_001293091.3
Accession Version	NM_001293091.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-14
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform 4
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, AK296881.1, AC002558.2 and AA927469.1. On May 28, 2014 this sequence version replaced XM_005257895.1. Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and it thus differs in its 5' UTR and initiates translation at an alternate start codon, and it also uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296881.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280020.1
CDS	176..2467
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001293091.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293091.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001293091.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001280020.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu27731
Clone ID Related Accession (Same CDS sequence)	XM_024451052.1 , NM_001293091.2 , NM_001293091.1 , NM_001293091.3
Accession Version	NM_001293091.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY023336.1, AK296881.1, AC002558.2 and AA927469.1. On May 31, 2019 this sequence version replaced NM_001293091.2. Transcript Variant: This variant (4) lacks an alternate exon in the 5' region and it thus differs in its 5' UTR and initiates translation at an alternate start codon, and it also uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (4) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.224081.1, SRR1660805.109066.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001280020.1
CDS	159..2450
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001293091.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001293091.3

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353050.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339979.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu56777
Clone ID Related Accession (Same CDS sequence)	NM_001353050.2 , NM_001353050.1 , XM_011525550.2
Accession Version	NM_001353050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On Jun 1, 2019 this sequence version replaced NM_001353050.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.76091.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGAC TTTTCGTGTT 
TTACAGTCTG TTGCCAGAAT CGGCCAAACT GGAACAAAGT CTGTCTTCTC ACAGAGTGGA 
AATAGCCGAG AGGTAACTCC AATTCTTGCA CAAACACAAA GTTCTGGTCC ACAAACAAGT 
ACAACACCTC AGGTATTGAG CCCCACTATT ACATCTCCCC CAAACGCACT GCCTCGAAGA 
AGTTCACGAC TCTTTACTAG TGACAGCTCC ACAACCAAGG AGAATAGCAA AAAATTAAAA 
ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339979.1
CDS	159..2342
Translation	MASTKPLCLPRCGFPRRTPLCRSKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001353050.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353050.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGAC TTTTCGTGTT 
TTACAGTCTG TTGCCAGAAT CGGCCAAACT GGAACAAAGT CTGTCTTCTC ACAGAGTGGA 
AATAGCCGAG AGGTAACTCC AATTCTTGCA CAAACACAAA GTTCTGGTCC ACAAACAAGT 
ACAACACCTC AGGTATTGAG CCCCACTATT ACATCTCCCC CAAACGCACT GCCTCGAAGA 
AGTTCACGAC TCTTTACTAG TGACAGCTCC ACAACCAAGG AGAATAGCAA AAAATTAAAA 
ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( NM_001353050.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001339979.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant 8, mRNA.

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CloneID	OHu56777
Clone ID Related Accession (Same CDS sequence)	NM_001353050.2 , NM_001353050.1 , XM_011525550.2
Accession Version	NM_001353050.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC002558.2. On Jun 28, 2017 this sequence version replaced XM_011525550.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.76091.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGAC TTTTCGTGTT 
TTACAGTCTG TTGCCAGAAT CGGCCAAACT GGAACAAAGT CTGTCTTCTC ACAGAGTGGA 
AATAGCCGAG AGGTAACTCC AATTCTTGCA CAAACACAAA GTTCTGGTCC ACAAACAAGT 
ACAACACCTC AGGTATTGAG CCCCACTATT ACATCTCCCC CAAACGCACT GCCTCGAAGA 
AGTTCACGAC TCTTTACTAG TGACAGCTCC ACAACCAAGG AGAATAGCAA AAAATTAAAA 
ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001339979.1
CDS	203..2386
Translation	MASTKPLCLPRCGFPRRTPLCRSKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	NM_001353050.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353050.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGAC TTTTCGTGTT 
TTACAGTCTG TTGCCAGAAT CGGCCAAACT GGAACAAAGT CTGTCTTCTC ACAGAGTGGA 
AATAGCCGAG AGGTAACTCC AATTCTTGCA CAAACACAAA GTTCTGGTCC ACAAACAAGT 
ACAACACCTC AGGTATTGAG CCCCACTATT ACATCTCCCC CAAACGCACT GCCTCGAAGA 
AGTTCACGAC TCTTTACTAG TGACAGCTCC ACAACCAAGG AGAATAGCAA AAAATTAAAA 
ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_024451052.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306820.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X7, mRNA.

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CloneID	OHu27731
Clone ID Related Accession (Same CDS sequence)	XM_024451052.1 , NM_001293091.2 , NM_001293091.1 , NM_001293091.3
Accession Version	XM_024451052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306820.1
CDS	383..2674
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_024451052.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451052.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGAAGGT 
TTGATGAGCC TTCTTCGTGA AATGGGGAAA GGTTATTTAG CTTTGTGTTC ATACAACTGC 
AAAGAAGCTA TAAATATTTT GAGCCATCTA CCTTCTCACC ACTACAATAC TGGTTGGGTA 
CTGTGCCAAA TTGGAAGGGC CTATTTTGAA CTTTCAGAGT ACATGCAAGC TGAAAGAATA 
TTCTCAGAGG TTAGAAGGAT TGAGAATTAT AGAGTTGAAG GCATGGAGAT CTACTCTACA 
ACACTTTGGC ATCTTCAAAA AGATGTTGCT CTTTCAGTTC TGTCAAAAGA CTTAACAGAC 
ATGGATAAAA ATTCGCCAGA GGCCTGGTGT GCTGCAGGGA ACTGTTTCAG TCTGCAACGG 
GAACATGATA TTGCAATTAA ATTCTTCCAG AGAGCTATCC AAGTTGATCC AAATTACGCT 
TATGCCTATA CTCTATTAGG GCATGAGTTT GTCTTAACTG AAGAATTGGA CAAAGCATTA 
GCTTGTTTTC GAAATGCTAT CAGAGTCAAT CCTAGACATT ATAATGCATG GTATGGTTTA 
GGAATGATTT ATTACAAGCA AGAAAAATTC AGCCTTGCAG AAATGCATTT CCAAAAAGCG 
CTTGATATCA ACCCTCAAAG TTCAGTTTTA CTTTGCCACA TTGGAGTAGT TCAACATGCA 
CTGAAAAAAT CAGAGAAGGC TTTGGATACC CTAAACAAAG CCATTGTCAT TGATCCCAAG 
AACCCTCTAT GCAAATTTCA CAGAGCCTCA GTTTTATTTG CAAATGAAAA ATATAAGTCT 
GCTTTACAAG AACTTGAAGA ATTGAAACAA ATTGTTCCCA AAGAATCCCT CGTTTACTTC 
TTAATAGGAA AGGTTTACAA GAAGTTAGGT CAAACGCACC TCGCCCTGAT GAATTTCTCT 
TGGGCTATGG ATTTAGATCC TAAAGGAGCC AATAACCAGA TTAAAGAGGC AATTGATAAG 
CGTTATCTTC CAGATGATGA GGAGCCAATA ACCCAAGAAG AACAGATCAT GGGAACAGAT 
GAATCCCAGG AGAGCAGCAT GACAGATGCG GATGACACAC AACTTCATGC AGCTGAAAGT 
GATGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525546.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523848.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56774
Clone ID Related Accession (Same CDS sequence)	XM_011525546.2 , XM_011525546.3
Accession Version	XM_011525546.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523848.1
CDS	128..2617
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_011525546.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525546.2

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525546.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523848.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu56774
Clone ID Related Accession (Same CDS sequence)	XM_011525546.2 , XM_011525546.3
Accession Version	XM_011525546.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2490bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525546.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523848.1
CDS	102..2591
Translation	MTVLQEPVQAAIWQALNHYAYRDAVFLAERLYAEVHSEEALFLLATCYYRSGKAYKAYRLLKGHSCTTPQCKYLLAKCCVDLSKLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_011525546.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525546.3

ORF Insert Sequence:

ATGACGGTGC TGCAGGAACC CGTCCAGGCT GCTATATGGC AAGCACTAAA CCACTATGCT 
TACCGAGATG CGGTTTTCCT CGCAGAACGC CTTTATGCAG AAGTACACTC AGAAGAAGCC 
TTGTTTTTAC TGGCAACCTG TTATTACCGC TCAGGAAAGG CATATAAAGC ATATAGACTC 
TTGAAAGGAC ACAGTTGTAC TACACCGCAA TGCAAATACC TGCTTGCAAA ATGTTGTGTT 
GATCTCAGCA AGCTTGCAGA AGGGGAACAA ATCTTATCTG GTGGAGTGTT TAATAAGCAG 
AAAAGCCATG ATGATATTGT TACTGAGTTT GGTGATTCAG CTTGCTTTAC TCTTTCATTG 
TTGGGACATG TATATTGCAA GACAGATCGG CTTGCCAAAG GATCAGAATG TTACCAAAAG 
AGCCTTAGTT TAAATCCTTT CCTCTGGTCT CCCTTTGAAT CATTATGTGA AATAGGTGAA 
AAGCCAGATC CTGACCAAAC ATTTAAATTC ACATCTTTAC AGAACTTTAG CAACTGTCTG 
CCCAACTCTT GCACAACACA AGTACCTAAT CATAGTTTAT CTCACAGACA GCCTGAGACA 
GTTCTTACGG AAACACCCCA GGACACAATT GAATTAAACA GATTGAATTT AGAATCTTCC 
AATTCAAAGT ACTCCTTGAA TACAGATTCC TCAGTGTCTT ATATTGATTC AGCTGTAATT 
TCACCTGATA CTGTCCCACT GGGAACAGGA ACTTCCATAT TATCTAAACA GGTTCAAAAT 
AAACCAAAAA CTGGTCGAAG TTTATTAGGA GGACCAGCAG CTCTTAGTCC ATTAACCCCA 
AGTTTTGGGA TTTTGCCATT AGAAACCCCA AGTCCTGGAG ATGGATCCTA TTTACAAAAC 
TACACTAATA CACCTCCTGT AATTGATGTG CCATCCACCG GAGCCCCTTC AAAAAAGACT 
TTTCGTGTTT TACAGTCTGT TGCCAGAATC GGCCAAACTG GAACAAAGTC TGTCTTCTCA 
CAGAGTGGAA ATAGCCGAGA GGTAACTCCA ATTCTTGCAC AAACACAAAG TTCTGGTCCA 
CAAACAAGTA CAACACCTCA GGTATTGAGC CCCACTATTA CATCTCCCCC AAACGCACTG 
CCTCGAAGAA GTTCACGACT CTTTACTAGT GACAGCTCCA CAACCAAGGA GAATAGCAAA 
AAATTAAAAA TGAAGTTTCC ACCTAAAATC CCAAACAGAA AAACAAAAAG TAAAACTAAT 
AAAGGAGGAA TAACTCAACC TAACATAAAT GATAGCCTGG AAATTACAAA ATTGGACTCT 
TCCATCATTT CAGAAGGGAA AATATCCACA ATCACACCTC AGATTCAGGC CTTTAATCTA 
CAAAAAGCAG CAGCAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525548.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523850.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu56776
Clone ID Related Accession (Same CDS sequence)	XM_011525548.2 , XM_011525548.3 , XM_011525549.2
Accession Version	XM_011525548.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525548.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGACTTTT 
CGTGTTTTAC AGTCTGTTGC CAGAATCGGC CAAACTGGAA CAAAGTCTGT CTTCTCACAG 
AGTGGAAATA GCCGAGAGGT AACTCCAATT CTTGCACAAA CACAAAGTTC TGGTCCACAA 
ACAAGTACAA CACCTCAGGT ATTGAGCCCC ACTATTACAT CTCCCCCAAA CGCACTGCCT 
CGAAGAAGTT CACGACTCTT TACTAGTGAC AGCTCCACAA CCAAGGAGAA TAGCAAAAAA 
TTAAAAATGA AGTTTCCACC TAAAATCCCA AACAGAAAAA CAAAAAGTAA AACTAATAAA 
GGAGGAATAA CTCAACCTAA CATAAATGAT AGCCTGGAAA TTACAAAATT GGACTCTTCC 
ATCATTTCAG AAGGGAAAAT ATCCACAATC ACACCTCAGA TTCAGGCCTT TAATCTACAA 
AAAGCAGCAG CAGAAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523850.1
CDS	196..2505
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_011525548.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525548.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGACTTTT 
CGTGTTTTAC AGTCTGTTGC CAGAATCGGC CAAACTGGAA CAAAGTCTGT CTTCTCACAG 
AGTGGAAATA GCCGAGAGGT AACTCCAATT CTTGCACAAA CACAAAGTTC TGGTCCACAA 
ACAAGTACAA CACCTCAGGT ATTGAGCCCC ACTATTACAT CTCCCCCAAA CGCACTGCCT 
CGAAGAAGTT CACGACTCTT TACTAGTGAC AGCTCCACAA CCAAGGAGAA TAGCAAAAAA 
TTAAAAATGA AGTTTCCACC TAAAATCCCA AACAGAAAAA CAAAAAGTAA AACTAATAAA 
GGAGGAATAA CTCAACCTAA CATAAATGAT AGCCTGGAAA TTACAAAATT GGACTCTTCC 
ATCATTTCAG AAGGGAAAAT ATCCACAATC ACACCTCAGA TTCAGGCCTT TAATCTACAA 
AAAGCAGCAG CAGAAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525548.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523850.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu56776
Clone ID Related Accession (Same CDS sequence)	XM_011525548.2 , XM_011525548.3 , XM_011525549.2
Accession Version	XM_011525548.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011525548.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGACTTTT 
CGTGTTTTAC AGTCTGTTGC CAGAATCGGC CAAACTGGAA CAAAGTCTGT CTTCTCACAG 
AGTGGAAATA GCCGAGAGGT AACTCCAATT CTTGCACAAA CACAAAGTTC TGGTCCACAA 
ACAAGTACAA CACCTCAGGT ATTGAGCCCC ACTATTACAT CTCCCCCAAA CGCACTGCCT 
CGAAGAAGTT CACGACTCTT TACTAGTGAC AGCTCCACAA CCAAGGAGAA TAGCAAAAAA 
TTAAAAATGA AGTTTCCACC TAAAATCCCA AACAGAAAAA CAAAAAGTAA AACTAATAAA 
GGAGGAATAA CTCAACCTAA CATAAATGAT AGCCTGGAAA TTACAAAATT GGACTCTTCC 
ATCATTTCAG AAGGGAAAAT ATCCACAATC ACACCTCAGA TTCAGGCCTT TAATCTACAA 
AAAGCAGCAG CAGAAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523850.1
CDS	116..2425
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_011525548.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525548.3

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGACTTTT 
CGTGTTTTAC AGTCTGTTGC CAGAATCGGC CAAACTGGAA CAAAGTCTGT CTTCTCACAG 
AGTGGAAATA GCCGAGAGGT AACTCCAATT CTTGCACAAA CACAAAGTTC TGGTCCACAA 
ACAAGTACAA CACCTCAGGT ATTGAGCCCC ACTATTACAT CTCCCCCAAA CGCACTGCCT 
CGAAGAAGTT CACGACTCTT TACTAGTGAC AGCTCCACAA CCAAGGAGAA TAGCAAAAAA 
TTAAAAATGA AGTTTCCACC TAAAATCCCA AACAGAAAAA CAAAAAGTAA AACTAATAAA 
GGAGGAATAA CTCAACCTAA CATAAATGAT AGCCTGGAAA TTACAAAATT GGACTCTTCC 
ATCATTTCAG AAGGGAAAAT ATCCACAATC ACACCTCAGA TTCAGGCCTT TAATCTACAA 
AAAGCAGCAG CAGAAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525549.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523851.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu56776
Clone ID Related Accession (Same CDS sequence)	XM_011525548.2 , XM_011525548.3 , XM_011525549.2
Accession Version	XM_011525549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGACTTTT 
CGTGTTTTAC AGTCTGTTGC CAGAATCGGC CAAACTGGAA CAAAGTCTGT CTTCTCACAG 
AGTGGAAATA GCCGAGAGGT AACTCCAATT CTTGCACAAA CACAAAGTTC TGGTCCACAA 
ACAAGTACAA CACCTCAGGT ATTGAGCCCC ACTATTACAT CTCCCCCAAA CGCACTGCCT 
CGAAGAAGTT CACGACTCTT TACTAGTGAC AGCTCCACAA CCAAGGAGAA TAGCAAAAAA 
TTAAAAATGA AGTTTCCACC TAAAATCCCA AACAGAAAAA CAAAAAGTAA AACTAATAAA 
GGAGGAATAA CTCAACCTAA CATAAATGAT AGCCTGGAAA TTACAAAATT GGACTCTTCC 
ATCATTTCAG AAGGGAAAAT ATCCACAATC ACACCTCAGA TTCAGGCCTT TAATCTACAA 
AAAGCAGCAG CAGAAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523851.1
CDS	319..2628
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_011525549.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525549.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGACTTTT 
CGTGTTTTAC AGTCTGTTGC CAGAATCGGC CAAACTGGAA CAAAGTCTGT CTTCTCACAG 
AGTGGAAATA GCCGAGAGGT AACTCCAATT CTTGCACAAA CACAAAGTTC TGGTCCACAA 
ACAAGTACAA CACCTCAGGT ATTGAGCCCC ACTATTACAT CTCCCCCAAA CGCACTGCCT 
CGAAGAAGTT CACGACTCTT TACTAGTGAC AGCTCCACAA CCAAGGAGAA TAGCAAAAAA 
TTAAAAATGA AGTTTCCACC TAAAATCCCA AACAGAAAAA CAAAAAGTAA AACTAATAAA 
GGAGGAATAA CTCAACCTAA CATAAATGAT AGCCTGGAAA TTACAAAATT GGACTCTTCC 
ATCATTTCAG AAGGGAAAAT ATCCACAATC ACACCTCAGA TTCAGGCCTT TAATCTACAA 
AAAGCAGCAG CAGAAGGTTT GATGAGCCTT CTTCGTGAAA TGGGGAAAGG TTATTTAGCT 
TTGTGTTCAT ACAACTGCAA AGAAGCTATA AATATTTTGA GCCATCTACC TTCTCACCAC 
TACAATACTG GTTGGGTACT GTGCCAAATT GGAAGGGCCT ATTTTGAACT TTCAGAGTAC 
ATGCAAGCTG AAAGAATATT CTCAGAGGTT AGAAGGATTG AGAATTATAG AGTTGAAGGC 
ATGGAGATCT ACTCTACAAC ACTTTGGCAT CTTCAAAAAG ATGTTGCTCT TTCAGTTCTG 
TCAAAAGACT TAACAGACAT GGATAAAAAT TCGCCAGAGG CCTGGTGTGC TGCAGGGAAC 
TGTTTCAGTC TGCAACGGGA ACATGATATT GCAATTAAAT TCTTCCAGAG AGCTATCCAA 
GTTGATCCAA ATTACGCTTA TGCCTATACT CTATTAGGGC ATGAGTTTGT CTTAACTGAA 
GAATTGGACA AAGCATTAGC TTGTTTTCGA AATGCTATCA GAGTCAATCC TAGACATTAT 
AATGCATGGT ATGGTTTAGG AATGATTTAT TACAAGCAAG AAAAATTCAG CCTTGCAGAA 
ATGCATTTCC AAAAAGCGCT TGATATCAAC CCTCAAAGTT CAGTTTTACT TTGCCACATT 
GGAGTAGTTC AACATGCACT GAAAAAATCA GAGAAGGCTT TGGATACCCT AAACAAAGCC 
ATTGTCATTG ATCCCAAGAA CCCTCTATGC AAATTTCACA GAGCCTCAGT TTTATTTGCA 
AATGAAAAAT ATAAGTCTGC TTTACAAGAA CTTGAAGAAT TGAAACAAAT TGTTCCCAAA 
GAATCCCTCG TTTACTTCTT AATAGGAAAG GTTTACAAGA AGTTAGGTCA AACGCACCTC 
GCCCTGATGA ATTTCTCTTG GGCTATGGAT TTAGATCCTA AAGGAGCCAA TAACCAGATT 
AAAGAGGCAA TTGATAAGCG TTATCTTCCA GATGATGAGG AGCCAATAAC CCAAGAAGAA 
CAGATCATGG GAACAGATGA ATCCCAGGAG AGCAGCATGA CAGATGCGGA TGACACACAA 
CTTCATGCAG CTGAAAGTGA TGAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_011525550.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011523852.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu56777
Clone ID Related Accession (Same CDS sequence)	NM_001353050.2 , NM_001353050.1 , XM_011525550.2
Accession Version	XM_011525550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	cell division cycle protein 27 homolog isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011525550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGAC TTTTCGTGTT 
TTACAGTCTG TTGCCAGAAT CGGCCAAACT GGAACAAAGT CTGTCTTCTC ACAGAGTGGA 
AATAGCCGAG AGGTAACTCC AATTCTTGCA CAAACACAAA GTTCTGGTCC ACAAACAAGT 
ACAACACCTC AGGTATTGAG CCCCACTATT ACATCTCCCC CAAACGCACT GCCTCGAAGA 
AGTTCACGAC TCTTTACTAG TGACAGCTCC ACAACCAAGG AGAATAGCAA AAAATTAAAA 
ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011523852.1
CDS	132..2315
Translation	MASTKPLCLPRCGFPRRTPLCRSKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKTFRVLQSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_011525550.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011525550.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGAC TTTTCGTGTT 
TTACAGTCTG TTGCCAGAAT CGGCCAAACT GGAACAAAGT CTGTCTTCTC ACAGAGTGGA 
AATAGCCGAG AGGTAACTCC AATTCTTGCA CAAACACAAA GTTCTGGTCC ACAAACAAGT 
ACAACACCTC AGGTATTGAG CCCCACTATT ACATCTCCCC CAAACGCACT GCCTCGAAGA 
AGTTCACGAC TCTTTACTAG TGACAGCTCC ACAACCAAGG AGAATAGCAA AAAATTAAAA 
ATGAAGTTTC CACCTAAAAT CCCAAACAGA AAAACAAAAA GTAAAACTAA TAAAGGAGGA 
ATAACTCAAC CTAACATAAA TGATAGCCTG GAAATTACAA AATTGGACTC TTCCATCATT 
TCAGAAGGGA AAATATCCAC AATCACACCT CAGATTCAGG CCTTTAATCT ACAAAAAGCA 
GCAGCAGAAG GTTTGATGAG CCTTCTTCGT GAAATGGGGA AAGGTTATTT AGCTTTGTGT 
TCATACAACT GCAAAGAAGC TATAAATATT TTGAGCCATC TACCTTCTCA CCACTACAAT 
ACTGGTTGGG TACTGTGCCA AATTGGAAGG GCCTATTTTG AACTTTCAGA GTACATGCAA 
GCTGAAAGAA TATTCTCAGA GGTTAGAAGG ATTGAGAATT ATAGAGTTGA AGGCATGGAG 
ATCTACTCTA CAACACTTTG GCATCTTCAA AAAGATGTTG CTCTTTCAGT TCTGTCAAAA 
GACTTAACAG ACATGGATAA AAATTCGCCA GAGGCCTGGT GTGCTGCAGG GAACTGTTTC 
AGTCTGCAAC GGGAACATGA TATTGCAATT AAATTCTTCC AGAGAGCTAT CCAAGTTGAT 
CCAAATTACG CTTATGCCTA TACTCTATTA GGGCATGAGT TTGTCTTAAC TGAAGAATTG 
GACAAAGCAT TAGCTTGTTT TCGAAATGCT ATCAGAGTCA ATCCTAGACA TTATAATGCA 
TGGTATGGTT TAGGAATGAT TTATTACAAG CAAGAAAAAT TCAGCCTTGC AGAAATGCAT 
TTCCAAAAAG CGCTTGATAT CAACCCTCAA AGTTCAGTTT TACTTTGCCA CATTGGAGTA 
GTTCAACATG CACTGAAAAA ATCAGAGAAG GCTTTGGATA CCCTAAACAA AGCCATTGTC 
ATTGATCCCA AGAACCCTCT ATGCAAATTT CACAGAGCCT CAGTTTTATT TGCAAATGAA 
AAATATAAGT CTGCTTTACA AGAACTTGAA GAATTGAAAC AAATTGTTCC CAAAGAATCC 
CTCGTTTACT TCTTAATAGG AAAGGTTTAC AAGAAGTTAG GTCAAACGCA CCTCGCCCTG 
ATGAATTTCT CTTGGGCTAT GGATTTAGAT CCTAAAGGAG CCAATAACCA GATTAAAGAG 
GCAATTGATA AGCGTTATCT TCCAGATGAT GAGGAGCCAA TAACCCAAGA AGAACAGATC 
ATGGGAACAG ATGAATCCCA GGAGAGCAGC ATGACAGATG CGGATGACAC ACAACTTCAT 
GCAGCTGAAA GTGATGAATT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025484.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880973.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu87728
Clone ID Related Accession (Same CDS sequence)	XM_017025484.2 , XM_017025484.1
Accession Version	XM_017025484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGGTTTG 
ATGAGCCTTC TTCGTGAAAT GGGGAAAGGT TATTTAGCTT TGTGTTCATA CAACTGCAAA 
GAAGCTATAA ATATTTTGAG CCATCTACCT TCTCACCACT ACAATACTGG TTGGGTACTG 
TGCCAAATTG GAAGGGCCTA TTTTGAACTT TCAGAGTACA TGCAAGCTGA AAGAATATTC 
TCAGAGGTTA GAAGGATTGA GAATTATAGA GTTGAAGGCA TGGAGATCTA CTCTACAACA 
CTTTGGCATC TTCAAAAAGA TGTTGCTCTT TCAGTTCTGT CAAAAGACTT AACAGACATG 
GATAAAAATT CGCCAGAGGC CTGGTGTGCT GCAGGGAACT GTTTCAGTCT GCAACGGGAA 
CATGATATTG CAATTAAATT CTTCCAGAGA GCTATCCAAG TTGATCCAAA TTACGCTTAT 
GCCTATACTC TATTAGGGCA TGAGTTTGTC TTAACTGAAG AATTGGACAA AGCATTAGCT 
TGTTTTCGAA ATGCTATCAG AGTCAATCCT AGACATTATA ATGCATGGTA TGGTTTAGGA 
ATGATTTATT ACAAGCAAGA AAAATTCAGC CTTGCAGAAA TGCATTTCCA AAAAGCGCTT 
GATATCAACC CTCAAAGTTC AGTTTTACTT TGCCACATTG GAGTAGTTCA ACATGCACTG 
AAAAAATCAG AGAAGGCTTT GGATACCCTA AACAAAGCCA TTGTCATTGA TCCCAAGAAC 
CCTCTATGCA AATTTCACAG AGCCTCAGTT TTATTTGCAA ATGAAAAATA TAAGTCTGCT 
TTACAAGAAC TTGAAGAATT GAAACAAATT GTTCCCAAAG AATCCCTCGT TTACTTCTTA 
ATAGGAAAGG TTTACAAGAA GTTAGGTCAA ACGCACCTCG CCCTGATGAA TTTCTCTTGG 
GCTATGGATT TAGATCCTAA AGGAGCCAAT AACCAGATTA AAGAGGCAAT TGATAAGCGT 
TATCTTCCAG ATGATGAGGA GCCAATAACC CAAGAAGAAC AGATCATGGG AACAGATGAA 
TCCCAGGAGA GCAGCATGAC AGATGCGGAT GACACACAAC TTCATGCAGC TGAAAGTGAT 
GAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880973.1
CDS	116..2404
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_017025484.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025484.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGGTTTG 
ATGAGCCTTC TTCGTGAAAT GGGGAAAGGT TATTTAGCTT TGTGTTCATA CAACTGCAAA 
GAAGCTATAA ATATTTTGAG CCATCTACCT TCTCACCACT ACAATACTGG TTGGGTACTG 
TGCCAAATTG GAAGGGCCTA TTTTGAACTT TCAGAGTACA TGCAAGCTGA AAGAATATTC 
TCAGAGGTTA GAAGGATTGA GAATTATAGA GTTGAAGGCA TGGAGATCTA CTCTACAACA 
CTTTGGCATC TTCAAAAAGA TGTTGCTCTT TCAGTTCTGT CAAAAGACTT AACAGACATG 
GATAAAAATT CGCCAGAGGC CTGGTGTGCT GCAGGGAACT GTTTCAGTCT GCAACGGGAA 
CATGATATTG CAATTAAATT CTTCCAGAGA GCTATCCAAG TTGATCCAAA TTACGCTTAT 
GCCTATACTC TATTAGGGCA TGAGTTTGTC TTAACTGAAG AATTGGACAA AGCATTAGCT 
TGTTTTCGAA ATGCTATCAG AGTCAATCCT AGACATTATA ATGCATGGTA TGGTTTAGGA 
ATGATTTATT ACAAGCAAGA AAAATTCAGC CTTGCAGAAA TGCATTTCCA AAAAGCGCTT 
GATATCAACC CTCAAAGTTC AGTTTTACTT TGCCACATTG GAGTAGTTCA ACATGCACTG 
AAAAAATCAG AGAAGGCTTT GGATACCCTA AACAAAGCCA TTGTCATTGA TCCCAAGAAC 
CCTCTATGCA AATTTCACAG AGCCTCAGTT TTATTTGCAA ATGAAAAATA TAAGTCTGCT 
TTACAAGAAC TTGAAGAATT GAAACAAATT GTTCCCAAAG AATCCCTCGT TTACTTCTTA 
ATAGGAAAGG TTTACAAGAA GTTAGGTCAA ACGCACCTCG CCCTGATGAA TTTCTCTTGG 
GCTATGGATT TAGATCCTAA AGGAGCCAAT AACCAGATTA AAGAGGCAAT TGATAAGCGT 
TATCTTCCAG ATGATGAGGA GCCAATAACC CAAGAAGAAC AGATCATGGG AACAGATGAA 
TCCCAGGAGA GCAGCATGAC AGATGCGGAT GACACACAAC TTCATGCAGC TGAAAGTGAT 
GAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025484.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880973.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu87728
Clone ID Related Accession (Same CDS sequence)	XM_017025484.2 , XM_017025484.1
Accession Version	XM_017025484.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGGTTTG 
ATGAGCCTTC TTCGTGAAAT GGGGAAAGGT TATTTAGCTT TGTGTTCATA CAACTGCAAA 
GAAGCTATAA ATATTTTGAG CCATCTACCT TCTCACCACT ACAATACTGG TTGGGTACTG 
TGCCAAATTG GAAGGGCCTA TTTTGAACTT TCAGAGTACA TGCAAGCTGA AAGAATATTC 
TCAGAGGTTA GAAGGATTGA GAATTATAGA GTTGAAGGCA TGGAGATCTA CTCTACAACA 
CTTTGGCATC TTCAAAAAGA TGTTGCTCTT TCAGTTCTGT CAAAAGACTT AACAGACATG 
GATAAAAATT CGCCAGAGGC CTGGTGTGCT GCAGGGAACT GTTTCAGTCT GCAACGGGAA 
CATGATATTG CAATTAAATT CTTCCAGAGA GCTATCCAAG TTGATCCAAA TTACGCTTAT 
GCCTATACTC TATTAGGGCA TGAGTTTGTC TTAACTGAAG AATTGGACAA AGCATTAGCT 
TGTTTTCGAA ATGCTATCAG AGTCAATCCT AGACATTATA ATGCATGGTA TGGTTTAGGA 
ATGATTTATT ACAAGCAAGA AAAATTCAGC CTTGCAGAAA TGCATTTCCA AAAAGCGCTT 
GATATCAACC CTCAAAGTTC AGTTTTACTT TGCCACATTG GAGTAGTTCA ACATGCACTG 
AAAAAATCAG AGAAGGCTTT GGATACCCTA AACAAAGCCA TTGTCATTGA TCCCAAGAAC 
CCTCTATGCA AATTTCACAG AGCCTCAGTT TTATTTGCAA ATGAAAAATA TAAGTCTGCT 
TTACAAGAAC TTGAAGAATT GAAACAAATT GTTCCCAAAG AATCCCTCGT TTACTTCTTA 
ATAGGAAAGG TTTACAAGAA GTTAGGTCAA ACGCACCTCG CCCTGATGAA TTTCTCTTGG 
GCTATGGATT TAGATCCTAA AGGAGCCAAT AACCAGATTA AAGAGGCAAT TGATAAGCGT 
TATCTTCCAG ATGATGAGGA GCCAATAACC CAAGAAGAAC AGATCATGGG AACAGATGAA 
TCCCAGGAGA GCAGCATGAC AGATGCGGAT GACACACAAC TTCATGCAGC TGAAAGTGAT 
GAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880973.1
CDS	192..2480
Translation	MASTKPLCLPRCGFPRRTPLCRRLAEGEQILSGGVFNKQKSHDDIVTEFGDSACFTLSLLGHVYCKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_017025484.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025484.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGGC TTGCAGAAGG GGAACAAATC TTATCTGGTG GAGTGTTTAA TAAGCAGAAA 
AGCCATGATG ATATTGTTAC TGAGTTTGGT GATTCAGCTT GCTTTACTCT TTCATTGTTG 
GGACATGTAT ATTGCAAGAC AGATCGGCTT GCCAAAGGAT CAGAATGTTA CCAAAAGAGC 
CTTAGTTTAA ATCCTTTCCT CTGGTCTCCC TTTGAATCAT TATGTGAAAT AGGTGAAAAG 
CCAGATCCTG ACCAAACATT TAAATTCACA TCTTTACAGA ACTTTAGCAA CTGTCTGCCC 
AACTCTTGCA CAACACAAGT ACCTAATCAT AGTTTATCTC ACAGACAGCC TGAGACAGTT 
CTTACGGAAA CACCCCAGGA CACAATTGAA TTAAACAGAT TGAATTTAGA ATCTTCCAAT 
TCAAAGTACT CCTTGAATAC AGATTCCTCA GTGTCTTATA TTGATTCAGC TGTAATTTCA 
CCTGATACTG TCCCACTGGG AACAGGAACT TCCATATTAT CTAAACAGGT TCAAAATAAA 
CCAAAAACTG GTCGAAGTTT ATTAGGAGGA CCAGCAGCTC TTAGTCCATT AACCCCAAGT 
TTTGGGATTT TGCCATTAGA AACCCCAAGT CCTGGAGATG GATCCTATTT ACAAAACTAC 
ACTAATACAC CTCCTGTAAT TGATGTGCCA TCCACCGGAG CCCCTTCAAA AAAGTCTGTT 
GCCAGAATCG GCCAAACTGG AACAAAGTCT GTCTTCTCAC AGAGTGGAAA TAGCCGAGAG 
GTAACTCCAA TTCTTGCACA AACACAAAGT TCTGGTCCAC AAACAAGTAC AACACCTCAG 
GTATTGAGCC CCACTATTAC ATCTCCCCCA AACGCACTGC CTCGAAGAAG TTCACGACTC 
TTTACTAGTG ACAGCTCCAC AACCAAGGAG AATAGCAAAA AATTAAAAAT GAAGTTTCCA 
CCTAAAATCC CAAACAGAAA AACAAAAAGT AAAACTAATA AAGGAGGAAT AACTCAACCT 
AACATAAATG ATAGCCTGGA AATTACAAAA TTGGACTCTT CCATCATTTC AGAAGGGAAA 
ATATCCACAA TCACACCTCA GATTCAGGCC TTTAATCTAC AAAAAGCAGC AGCAGGTTTG 
ATGAGCCTTC TTCGTGAAAT GGGGAAAGGT TATTTAGCTT TGTGTTCATA CAACTGCAAA 
GAAGCTATAA ATATTTTGAG CCATCTACCT TCTCACCACT ACAATACTGG TTGGGTACTG 
TGCCAAATTG GAAGGGCCTA TTTTGAACTT TCAGAGTACA TGCAAGCTGA AAGAATATTC 
TCAGAGGTTA GAAGGATTGA GAATTATAGA GTTGAAGGCA TGGAGATCTA CTCTACAACA 
CTTTGGCATC TTCAAAAAGA TGTTGCTCTT TCAGTTCTGT CAAAAGACTT AACAGACATG 
GATAAAAATT CGCCAGAGGC CTGGTGTGCT GCAGGGAACT GTTTCAGTCT GCAACGGGAA 
CATGATATTG CAATTAAATT CTTCCAGAGA GCTATCCAAG TTGATCCAAA TTACGCTTAT 
GCCTATACTC TATTAGGGCA TGAGTTTGTC TTAACTGAAG AATTGGACAA AGCATTAGCT 
TGTTTTCGAA ATGCTATCAG AGTCAATCCT AGACATTATA ATGCATGGTA TGGTTTAGGA 
ATGATTTATT ACAAGCAAGA AAAATTCAGC CTTGCAGAAA TGCATTTCCA AAAAGCGCTT 
GATATCAACC CTCAAAGTTC AGTTTTACTT TGCCACATTG GAGTAGTTCA ACATGCACTG 
AAAAAATCAG AGAAGGCTTT GGATACCCTA AACAAAGCCA TTGTCATTGA TCCCAAGAAC 
CCTCTATGCA AATTTCACAG AGCCTCAGTT TTATTTGCAA ATGAAAAATA TAAGTCTGCT 
TTACAAGAAC TTGAAGAATT GAAACAAATT GTTCCCAAAG AATCCCTCGT TTACTTCTTA 
ATAGGAAAGG TTTACAAGAA GTTAGGTCAA ACGCACCTCG CCCTGATGAA TTTCTCTTGG 
GCTATGGATT TAGATCCTAA AGGAGCCAAT AACCAGATTA AAGAGGCAAT TGATAAGCGT 
TATCTTCCAG ATGATGAGGA GCCAATAACC CAAGAAGAAC AGATCATGGG AACAGATGAA 
TCCCAGGAGA GCAGCATGAC AGATGCGGAT GACACACAAC TTCATGCAGC TGAAAGTGAT 
GAATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025485.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880974.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X9, mRNA.

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CloneID	OHu87729
Clone ID Related Accession (Same CDS sequence)	XM_017025485.2 , XM_017025485.1
Accession Version	XM_017025485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGTC TGTTGCCAGA 
ATCGGCCAAA CTGGAACAAA GTCTGTCTTC TCACAGAGTG GAAATAGCCG AGAGGTAACT 
CCAATTCTTG CACAAACACA AAGTTCTGGT CCACAAACAA GTACAACACC TCAGGTATTG 
AGCCCCACTA TTACATCTCC CCCAAACGCA CTGCCTCGAA GAAGTTCACG ACTCTTTACT 
AGTGACAGCT CCACAACCAA GGAGAATAGC AAAAAATTAA AAATGAAGTT TCCACCTAAA 
ATCCCAAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG GAATAACTCA ACCTAACATA 
AATGATAGCC TGGAAATTAC AAAATTGGAC TCTTCCATCA TTTCAGAAGG GAAAATATCC 
ACAATCACAC CTCAGATTCA GGCCTTTAAT CTACAAAAAG CAGCAGCAGA AGGTTTGATG 
AGCCTTCTTC GTGAAATGGG GAAAGGTTAT TTAGCTTTGT GTTCATACAA CTGCAAAGAA 
GCTATAAATA TTTTGAGCCA TCTACCTTCT CACCACTACA ATACTGGTTG GGTACTGTGC 
CAAATTGGAA GGGCCTATTT TGAACTTTCA GAGTACATGC AAGCTGAAAG AATATTCTCA 
GAGGTTAGAA GGATTGAGAA TTATAGAGTT GAAGGCATGG AGATCTACTC TACAACACTT 
TGGCATCTTC AAAAAGATGT TGCTCTTTCA GTTCTGTCAA AAGACTTAAC AGACATGGAT 
AAAAATTCGC CAGAGGCCTG GTGTGCTGCA GGGAACTGTT TCAGTCTGCA ACGGGAACAT 
GATATTGCAA TTAAATTCTT CCAGAGAGCT ATCCAAGTTG ATCCAAATTA CGCTTATGCC 
TATACTCTAT TAGGGCATGA GTTTGTCTTA ACTGAAGAAT TGGACAAAGC ATTAGCTTGT 
TTTCGAAATG CTATCAGAGT CAATCCTAGA CATTATAATG CATGGTATGG TTTAGGAATG 
ATTTATTACA AGCAAGAAAA ATTCAGCCTT GCAGAAATGC ATTTCCAAAA AGCGCTTGAT 
ATCAACCCTC AAAGTTCAGT TTTACTTTGC CACATTGGAG TAGTTCAACA TGCACTGAAA 
AAATCAGAGA AGGCTTTGGA TACCCTAAAC AAAGCCATTG TCATTGATCC CAAGAACCCT 
CTATGCAAAT TTCACAGAGC CTCAGTTTTA TTTGCAAATG AAAAATATAA GTCTGCTTTA 
CAAGAACTTG AAGAATTGAA ACAAATTGTT CCCAAAGAAT CCCTCGTTTA CTTCTTAATA 
GGAAAGGTTT ACAAGAAGTT AGGTCAAACG CACCTCGCCC TGATGAATTT CTCTTGGGCT 
ATGGATTTAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG AGGCAATTGA TAAGCGTTAT 
CTTCCAGATG ATGAGGAGCC AATAACCCAA GAAGAACAGA TCATGGGAAC AGATGAATCC 
CAGGAGAGCA GCATGACAGA TGCGGATGAC ACACAACTTC ATGCAGCTGA AAGTGATGAA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880974.1
CDS	116..2281
Translation	MASTKPLCLPRCGFPRRTPLCRSKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_017025485.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025485.2

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGTC TGTTGCCAGA 
ATCGGCCAAA CTGGAACAAA GTCTGTCTTC TCACAGAGTG GAAATAGCCG AGAGGTAACT 
CCAATTCTTG CACAAACACA AAGTTCTGGT CCACAAACAA GTACAACACC TCAGGTATTG 
AGCCCCACTA TTACATCTCC CCCAAACGCA CTGCCTCGAA GAAGTTCACG ACTCTTTACT 
AGTGACAGCT CCACAACCAA GGAGAATAGC AAAAAATTAA AAATGAAGTT TCCACCTAAA 
ATCCCAAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG GAATAACTCA ACCTAACATA 
AATGATAGCC TGGAAATTAC AAAATTGGAC TCTTCCATCA TTTCAGAAGG GAAAATATCC 
ACAATCACAC CTCAGATTCA GGCCTTTAAT CTACAAAAAG CAGCAGCAGA AGGTTTGATG 
AGCCTTCTTC GTGAAATGGG GAAAGGTTAT TTAGCTTTGT GTTCATACAA CTGCAAAGAA 
GCTATAAATA TTTTGAGCCA TCTACCTTCT CACCACTACA ATACTGGTTG GGTACTGTGC 
CAAATTGGAA GGGCCTATTT TGAACTTTCA GAGTACATGC AAGCTGAAAG AATATTCTCA 
GAGGTTAGAA GGATTGAGAA TTATAGAGTT GAAGGCATGG AGATCTACTC TACAACACTT 
TGGCATCTTC AAAAAGATGT TGCTCTTTCA GTTCTGTCAA AAGACTTAAC AGACATGGAT 
AAAAATTCGC CAGAGGCCTG GTGTGCTGCA GGGAACTGTT TCAGTCTGCA ACGGGAACAT 
GATATTGCAA TTAAATTCTT CCAGAGAGCT ATCCAAGTTG ATCCAAATTA CGCTTATGCC 
TATACTCTAT TAGGGCATGA GTTTGTCTTA ACTGAAGAAT TGGACAAAGC ATTAGCTTGT 
TTTCGAAATG CTATCAGAGT CAATCCTAGA CATTATAATG CATGGTATGG TTTAGGAATG 
ATTTATTACA AGCAAGAAAA ATTCAGCCTT GCAGAAATGC ATTTCCAAAA AGCGCTTGAT 
ATCAACCCTC AAAGTTCAGT TTTACTTTGC CACATTGGAG TAGTTCAACA TGCACTGAAA 
AAATCAGAGA AGGCTTTGGA TACCCTAAAC AAAGCCATTG TCATTGATCC CAAGAACCCT 
CTATGCAAAT TTCACAGAGC CTCAGTTTTA TTTGCAAATG AAAAATATAA GTCTGCTTTA 
CAAGAACTTG AAGAATTGAA ACAAATTGTT CCCAAAGAAT CCCTCGTTTA CTTCTTAATA 
GGAAAGGTTT ACAAGAAGTT AGGTCAAACG CACCTCGCCC TGATGAATTT CTCTTGGGCT 
ATGGATTTAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG AGGCAATTGA TAAGCGTTAT 
CTTCCAGATG ATGAGGAGCC AATAACCCAA GAAGAACAGA TCATGGGAAC AGATGAATCC 
CAGGAGAGCA GCATGACAGA TGCGGATGAC ACACAACTTC ATGCAGCTGA AAGTGATGAA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025485.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880974.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu87729
Clone ID Related Accession (Same CDS sequence)	XM_017025485.2 , XM_017025485.1
Accession Version	XM_017025485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGTC TGTTGCCAGA 
ATCGGCCAAA CTGGAACAAA GTCTGTCTTC TCACAGAGTG GAAATAGCCG AGAGGTAACT 
CCAATTCTTG CACAAACACA AAGTTCTGGT CCACAAACAA GTACAACACC TCAGGTATTG 
AGCCCCACTA TTACATCTCC CCCAAACGCA CTGCCTCGAA GAAGTTCACG ACTCTTTACT 
AGTGACAGCT CCACAACCAA GGAGAATAGC AAAAAATTAA AAATGAAGTT TCCACCTAAA 
ATCCCAAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG GAATAACTCA ACCTAACATA 
AATGATAGCC TGGAAATTAC AAAATTGGAC TCTTCCATCA TTTCAGAAGG GAAAATATCC 
ACAATCACAC CTCAGATTCA GGCCTTTAAT CTACAAAAAG CAGCAGCAGA AGGTTTGATG 
AGCCTTCTTC GTGAAATGGG GAAAGGTTAT TTAGCTTTGT GTTCATACAA CTGCAAAGAA 
GCTATAAATA TTTTGAGCCA TCTACCTTCT CACCACTACA ATACTGGTTG GGTACTGTGC 
CAAATTGGAA GGGCCTATTT TGAACTTTCA GAGTACATGC AAGCTGAAAG AATATTCTCA 
GAGGTTAGAA GGATTGAGAA TTATAGAGTT GAAGGCATGG AGATCTACTC TACAACACTT 
TGGCATCTTC AAAAAGATGT TGCTCTTTCA GTTCTGTCAA AAGACTTAAC AGACATGGAT 
AAAAATTCGC CAGAGGCCTG GTGTGCTGCA GGGAACTGTT TCAGTCTGCA ACGGGAACAT 
GATATTGCAA TTAAATTCTT CCAGAGAGCT ATCCAAGTTG ATCCAAATTA CGCTTATGCC 
TATACTCTAT TAGGGCATGA GTTTGTCTTA ACTGAAGAAT TGGACAAAGC ATTAGCTTGT 
TTTCGAAATG CTATCAGAGT CAATCCTAGA CATTATAATG CATGGTATGG TTTAGGAATG 
ATTTATTACA AGCAAGAAAA ATTCAGCCTT GCAGAAATGC ATTTCCAAAA AGCGCTTGAT 
ATCAACCCTC AAAGTTCAGT TTTACTTTGC CACATTGGAG TAGTTCAACA TGCACTGAAA 
AAATCAGAGA AGGCTTTGGA TACCCTAAAC AAAGCCATTG TCATTGATCC CAAGAACCCT 
CTATGCAAAT TTCACAGAGC CTCAGTTTTA TTTGCAAATG AAAAATATAA GTCTGCTTTA 
CAAGAACTTG AAGAATTGAA ACAAATTGTT CCCAAAGAAT CCCTCGTTTA CTTCTTAATA 
GGAAAGGTTT ACAAGAAGTT AGGTCAAACG CACCTCGCCC TGATGAATTT CTCTTGGGCT 
ATGGATTTAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG AGGCAATTGA TAAGCGTTAT 
CTTCCAGATG ATGAGGAGCC AATAACCCAA GAAGAACAGA TCATGGGAAC AGATGAATCC 
CAGGAGAGCA GCATGACAGA TGCGGATGAC ACACAACTTC ATGCAGCTGA AAGTGATGAA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880974.1
CDS	193..2358
Translation	MASTKPLCLPRCGFPRRTPLCRSKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAEGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_017025485.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025485.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGTC TGTTGCCAGA 
ATCGGCCAAA CTGGAACAAA GTCTGTCTTC TCACAGAGTG GAAATAGCCG AGAGGTAACT 
CCAATTCTTG CACAAACACA AAGTTCTGGT CCACAAACAA GTACAACACC TCAGGTATTG 
AGCCCCACTA TTACATCTCC CCCAAACGCA CTGCCTCGAA GAAGTTCACG ACTCTTTACT 
AGTGACAGCT CCACAACCAA GGAGAATAGC AAAAAATTAA AAATGAAGTT TCCACCTAAA 
ATCCCAAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG GAATAACTCA ACCTAACATA 
AATGATAGCC TGGAAATTAC AAAATTGGAC TCTTCCATCA TTTCAGAAGG GAAAATATCC 
ACAATCACAC CTCAGATTCA GGCCTTTAAT CTACAAAAAG CAGCAGCAGA AGGTTTGATG 
AGCCTTCTTC GTGAAATGGG GAAAGGTTAT TTAGCTTTGT GTTCATACAA CTGCAAAGAA 
GCTATAAATA TTTTGAGCCA TCTACCTTCT CACCACTACA ATACTGGTTG GGTACTGTGC 
CAAATTGGAA GGGCCTATTT TGAACTTTCA GAGTACATGC AAGCTGAAAG AATATTCTCA 
GAGGTTAGAA GGATTGAGAA TTATAGAGTT GAAGGCATGG AGATCTACTC TACAACACTT 
TGGCATCTTC AAAAAGATGT TGCTCTTTCA GTTCTGTCAA AAGACTTAAC AGACATGGAT 
AAAAATTCGC CAGAGGCCTG GTGTGCTGCA GGGAACTGTT TCAGTCTGCA ACGGGAACAT 
GATATTGCAA TTAAATTCTT CCAGAGAGCT ATCCAAGTTG ATCCAAATTA CGCTTATGCC 
TATACTCTAT TAGGGCATGA GTTTGTCTTA ACTGAAGAAT TGGACAAAGC ATTAGCTTGT 
TTTCGAAATG CTATCAGAGT CAATCCTAGA CATTATAATG CATGGTATGG TTTAGGAATG 
ATTTATTACA AGCAAGAAAA ATTCAGCCTT GCAGAAATGC ATTTCCAAAA AGCGCTTGAT 
ATCAACCCTC AAAGTTCAGT TTTACTTTGC CACATTGGAG TAGTTCAACA TGCACTGAAA 
AAATCAGAGA AGGCTTTGGA TACCCTAAAC AAAGCCATTG TCATTGATCC CAAGAACCCT 
CTATGCAAAT TTCACAGAGC CTCAGTTTTA TTTGCAAATG AAAAATATAA GTCTGCTTTA 
CAAGAACTTG AAGAATTGAA ACAAATTGTT CCCAAAGAAT CCCTCGTTTA CTTCTTAATA 
GGAAAGGTTT ACAAGAAGTT AGGTCAAACG CACCTCGCCC TGATGAATTT CTCTTGGGCT 
ATGGATTTAG ATCCTAAAGG AGCCAATAAC CAGATTAAAG AGGCAATTGA TAAGCGTTAT 
CTTCCAGATG ATGAGGAGCC AATAACCCAA GAAGAACAGA TCATGGGAAC AGATGAATCC 
CAGGAGAGCA GCATGACAGA TGCGGATGAC ACACAACTTC ATGCAGCTGA AAGTGATGAA 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC27 ( XM_017025486.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880975.1 Homo sapiens cell division cycle 27 (CDC27), transcript variant X10, mRNA.

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CloneID	OHu87730
Clone ID Related Accession (Same CDS sequence)	XM_017025486.1
Accession Version	XM_017025486.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cell division cycle protein 27 homolog isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGTC TGTTGCCAGA 
ATCGGCCAAA CTGGAACAAA GTCTGTCTTC TCACAGAGTG GAAATAGCCG AGAGGTAACT 
CCAATTCTTG CACAAACACA AAGTTCTGGT CCACAAACAA GTACAACACC TCAGGTATTG 
AGCCCCACTA TTACATCTCC CCCAAACGCA CTGCCTCGAA GAAGTTCACG ACTCTTTACT 
AGTGACAGCT CCACAACCAA GGAGAATAGC AAAAAATTAA AAATGAAGTT TCCACCTAAA 
ATCCCAAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG GAATAACTCA ACCTAACATA 
AATGATAGCC TGGAAATTAC AAAATTGGAC TCTTCCATCA TTTCAGAAGG GAAAATATCC 
ACAATCACAC CTCAGATTCA GGCCTTTAAT CTACAAAAAG CAGCAGCAGG TTTGATGAGC 
CTTCTTCGTG AAATGGGGAA AGGTTATTTA GCTTTGTGTT CATACAACTG CAAAGAAGCT 
ATAAATATTT TGAGCCATCT ACCTTCTCAC CACTACAATA CTGGTTGGGT ACTGTGCCAA 
ATTGGAAGGG CCTATTTTGA ACTTTCAGAG TACATGCAAG CTGAAAGAAT ATTCTCAGAG 
GTTAGAAGGA TTGAGAATTA TAGAGTTGAA GGCATGGAGA TCTACTCTAC AACACTTTGG 
CATCTTCAAA AAGATGTTGC TCTTTCAGTT CTGTCAAAAG ACTTAACAGA CATGGATAAA 
AATTCGCCAG AGGCCTGGTG TGCTGCAGGG AACTGTTTCA GTCTGCAACG GGAACATGAT 
ATTGCAATTA AATTCTTCCA GAGAGCTATC CAAGTTGATC CAAATTACGC TTATGCCTAT 
ACTCTATTAG GGCATGAGTT TGTCTTAACT GAAGAATTGG ACAAAGCATT AGCTTGTTTT 
CGAAATGCTA TCAGAGTCAA TCCTAGACAT TATAATGCAT GGTATGGTTT AGGAATGATT 
TATTACAAGC AAGAAAAATT CAGCCTTGCA GAAATGCATT TCCAAAAAGC GCTTGATATC 
AACCCTCAAA GTTCAGTTTT ACTTTGCCAC ATTGGAGTAG TTCAACATGC ACTGAAAAAA 
TCAGAGAAGG CTTTGGATAC CCTAAACAAA GCCATTGTCA TTGATCCCAA GAACCCTCTA 
TGCAAATTTC ACAGAGCCTC AGTTTTATTT GCAAATGAAA AATATAAGTC TGCTTTACAA 
GAACTTGAAG AATTGAAACA AATTGTTCCC AAAGAATCCC TCGTTTACTT CTTAATAGGA 
AAGGTTTACA AGAAGTTAGG TCAAACGCAC CTCGCCCTGA TGAATTTCTC TTGGGCTATG 
GATTTAGATC CTAAAGGAGC CAATAACCAG ATTAAAGAGG CAATTGATAA GCGTTATCTT 
CCAGATGATG AGGAGCCAAT AACCCAAGAA GAACAGATCA TGGGAACAGA TGAATCCCAG 
GAGAGCAGCA TGACAGATGC GGATGACACA CAACTTCATG CAGCTGAAAG TGATGAATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880975.1
CDS	113..2275
Translation	MASTKPLCLPRCGFPRRTPLCRSKTDRLAKGSECYQKSLSLNPFLWSPFESLCEIGEKPDPDQTFKFTSLQNFSNCLPNSCTTQVPNHSLSHRQPETVLTETPQDTIELNRLNLESSNSKYSLNTDSSVSYIDSAVISPDTVPLGTGTSILSKQVQNKPKTGRSLLGGPAALSPLTPSFGILPLETPSPGDGSYLQNYTNTPPVIDVPSTGAPSKKSVARIGQTGTKSVFSQSGNSREVTPILAQTQSSGPQTSTTPQVLSPTITSPPNALPRRSSRLFTSDSSTTKENSKKLKMKFPPKIPNRKTKSKTNKGGITQPNINDSLEITKLDSSIISEGKISTITPQIQAFNLQKAAAGLMSLLREMGKGYLALCSYNCKEAINILSHLPSHHYNTGWVLCQIGRAYFELSEYMQAERIFSEVRRIENYRVEGMEIYSTTLWHLQKDVALSVLSKDLTDMDKNSPEAWCAAGNCFSLQREHDIAIKFFQRAIQVDPNYAYAYTLLGHEFVLTEELDKALACFRNAIRVNPRHYNAWYGLGMIYYKQEKFSLAEMHFQKALDINPQSSVLLCHIGVVQHALKKSEKALDTLNKAIVIDPKNPLCKFHRASVLFANEKYKSALQELEELKQIVPKESLVYFLIGKVYKKLGQTHLALMNFSWAMDLDPKGANNQIKEAIDKRYLPDDEEPITQEEQIMGTDESQESSMTDADDTQLHAAESDEF

Target ORF information:

RefSeq Version	XM_017025486.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 27 (CDC27), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025486.1

ORF Insert Sequence:

ATGGCAAGCA CTAAACCACT ATGCTTACCG AGATGCGGTT TTCCTCGCAG AACGCCTTTA 
TGCAGAAGCA AGACAGATCG GCTTGCCAAA GGATCAGAAT GTTACCAAAA GAGCCTTAGT 
TTAAATCCTT TCCTCTGGTC TCCCTTTGAA TCATTATGTG AAATAGGTGA AAAGCCAGAT 
CCTGACCAAA CATTTAAATT CACATCTTTA CAGAACTTTA GCAACTGTCT GCCCAACTCT 
TGCACAACAC AAGTACCTAA TCATAGTTTA TCTCACAGAC AGCCTGAGAC AGTTCTTACG 
GAAACACCCC AGGACACAAT TGAATTAAAC AGATTGAATT TAGAATCTTC CAATTCAAAG 
TACTCCTTGA ATACAGATTC CTCAGTGTCT TATATTGATT CAGCTGTAAT TTCACCTGAT 
ACTGTCCCAC TGGGAACAGG AACTTCCATA TTATCTAAAC AGGTTCAAAA TAAACCAAAA 
ACTGGTCGAA GTTTATTAGG AGGACCAGCA GCTCTTAGTC CATTAACCCC AAGTTTTGGG 
ATTTTGCCAT TAGAAACCCC AAGTCCTGGA GATGGATCCT ATTTACAAAA CTACACTAAT 
ACACCTCCTG TAATTGATGT GCCATCCACC GGAGCCCCTT CAAAAAAGTC TGTTGCCAGA 
ATCGGCCAAA CTGGAACAAA GTCTGTCTTC TCACAGAGTG GAAATAGCCG AGAGGTAACT 
CCAATTCTTG CACAAACACA AAGTTCTGGT CCACAAACAA GTACAACACC TCAGGTATTG 
AGCCCCACTA TTACATCTCC CCCAAACGCA CTGCCTCGAA GAAGTTCACG ACTCTTTACT 
AGTGACAGCT CCACAACCAA GGAGAATAGC AAAAAATTAA AAATGAAGTT TCCACCTAAA 
ATCCCAAACA GAAAAACAAA AAGTAAAACT AATAAAGGAG GAATAACTCA ACCTAACATA 
AATGATAGCC TGGAAATTAC AAAATTGGAC TCTTCCATCA TTTCAGAAGG GAAAATATCC 
ACAATCACAC CTCAGATTCA GGCCTTTAAT CTACAAAAAG CAGCAGCAGG TTTGATGAGC 
CTTCTTCGTG AAATGGGGAA AGGTTATTTA GCTTTGTGTT CATACAACTG CAAAGAAGCT 
ATAAATATTT TGAGCCATCT ACCTTCTCAC CACTACAATA CTGGTTGGGT ACTGTGCCAA 
ATTGGAAGGG CCTATTTTGA ACTTTCAGAG TACATGCAAG CTGAAAGAAT ATTCTCAGAG 
GTTAGAAGGA TTGAGAATTA TAGAGTTGAA GGCATGGAGA TCTACTCTAC AACACTTTGG 
CATCTTCAAA AAGATGTTGC TCTTTCAGTT CTGTCAAAAG ACTTAACAGA CATGGATAAA 
AATTCGCCAG AGGCCTGGTG TGCTGCAGGG AACTGTTTCA GTCTGCAACG GGAACATGAT 
ATTGCAATTA AATTCTTCCA GAGAGCTATC CAAGTTGATC CAAATTACGC TTATGCCTAT 
ACTCTATTAG GGCATGAGTT TGTCTTAACT GAAGAATTGG ACAAAGCATT AGCTTGTTTT 
CGAAATGCTA TCAGAGTCAA TCCTAGACAT TATAATGCAT GGTATGGTTT AGGAATGATT 
TATTACAAGC AAGAAAAATT CAGCCTTGCA GAAATGCATT TCCAAAAAGC GCTTGATATC 
AACCCTCAAA GTTCAGTTTT ACTTTGCCAC ATTGGAGTAG TTCAACATGC ACTGAAAAAA 
TCAGAGAAGG CTTTGGATAC CCTAAACAAA GCCATTGTCA TTGATCCCAA GAACCCTCTA 
TGCAAATTTC ACAGAGCCTC AGTTTTATTT GCAAATGAAA AATATAAGTC TGCTTTACAA 
GAACTTGAAG AATTGAAACA AATTGTTCCC AAAGAATCCC TCGTTTACTT CTTAATAGGA 
AAGGTTTACA AGAAGTTAGG TCAAACGCAC CTCGCCCTGA TGAATTTCTC TTGGGCTATG 
GATTTAGATC CTAAAGGAGC CAATAACCAG ATTAAAGAGG CAATTGATAA GCGTTATCTT 
CCAGATGATG AGGAGCCAAT AACCCAAGAA GAACAGATCA TGGGAACAGA TGAATCCCAG 
GAGAGCAGCA TGACAGATGC GGATGACACA CAACTTCATG CAGCTGAAAG TGATGAATTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inhibition of Anaphase-Promoting Complex by Silence APC/C
Journal of cellular biochemistry118(10)3150-3157(2017 Oct)
Wang C,Su Z,Hou H,Li D,Pan Z,Tian W,Mo C

UBE2S associated with OSCC proliferation by promotion of P21 degradation via the ubiquitin-proteasome system.
Biochemical and biophysical research communications485(4)820-825(2017 04)
Yoshimura S,Kasamatsu A,Nakashima D,Iyoda M,Kasama H,Saito T,Takahara T,Endo-Sakamoto Y,Shiiba M,Tanzawa H,Uzawa K

mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27.
Oncotarget8(4)6304-6318(2017 Jan)
Feng Z,Zhang L,Zhou J,Zhou S,Li L,Guo X,Feng G,Ma Z,Huang W,Huang F

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

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CDC27 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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