GOLGA5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GOLGA5
Entrez Gene ID	9950
Full Name	golgin A5
Synonyms	GOLIM5,RFG5,ret-II
General protein information	Preferred Names golgin A5 Names golgin subfamily A member 5 RET-fused gene 5 protein cell proliferation-inducing gene 31 protein golgi autoantigen, golgin subfamily a, 5 golgi integral membrane protein 5 golgin-84
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q32.12
Summary	The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. A pseudogene of this gene is located on the short arm of chromosome 5. [provided by RefSeq, Jul 2013].
Disorder MIM:	606918

mRNA and Protein(s)

mRNA	Protein	Name
NM_005113.3	NP_005104.3	golgin subfamily A member 5
NM_005113.4	NP_005104.4	golgin subfamily A member 5
XM_011537420.2	XP_011535722.1	golgin subfamily A member 5 isoform X1
XM_011537420.3	XP_011535722.1	golgin subfamily A member 5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	GOLGA5	NP_005104.3
Pan troglodytes (chimpanzee)	GOLGA5	XP_001148857.1
Canis lupus familiaris (dog)	GOLGA5	XP_537356.2
Mus musculus (house mouse)	Golga5	NP_001185933.1
Gallus gallus (chicken)	GOLGA5	XP_421329.2
Danio rerio (zebrafish)	golga5	NP_998576.1
Caenorhabditis elegans (roundworm)	T24B1.1	NP_492137.1
Bos taurus (cattle)	GOLGA5	NP_001091476.1
Rattus norvegicus (Norway rat)	Golga5	NP_001028237.1
	GOLGA5	XP_001093961.1
Xenopus tropicalis (tropical clawed frog)	golga5	NP_001016232.1

Gene Ontology
Bibliography

Related articles in PubMed

Targeting of a chlamydial protease impedes intracellular bacterial growth.
Christian JG, Heymann J, Paschen SA, Vier J, Schauenburg L, Rupp J, Meyer TF, H?cker G, Heuer D
PLoS pathogens7(9)e1002283(2011 Sep)

Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde transport.
Sohda M, Misumi Y, Yamamoto A, Nakamura N, Ogata S, Sakisaka S, Hirose S, Ikehara Y, Oda K
Traffic (Copenhagen, Denmark)11(12)1552-66(2010 Dec)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction.
Heuer D, Rejman Lipinski A, Machuy N, Karlas A, Wehrens A, Siedler F, Brinkmann V, Meyer TF
Nature457(7230)731-5(2009 Feb)

Golgin tethers define subpopulations of COPI vesicles.
Malsam J, Satoh A, Pelletier L, Warren G
Science (New York, N.Y.)307(5712)1095-8(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Golgin-84 on coat protein I vesicles interacts with the conserved oligomeric Golgi complex before SNARE assembly.
Title: Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde transport.

involved in generating and maintaining the architecture of the Golgi apparatus
Title: Golgin-84 is a rab1 binding partner involved in Golgi structure.

The following GOLGA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GOLGA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20812	NM_005113.3 Latest version!	Homo sapiens golgin A5 (GOLGA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu63546	XM_011537420.3 Latest version!	Homo sapiens golgin A5 (GOLGA5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu107276	NM_005113.4 Latest version!	Homo sapiens golgin A5 (GOLGA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu63546	XM_011537420.2	Homo sapiens golgin A5 (GOLGA5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GOLGA5 ( NM_005113.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005104.3 Homo sapiens golgin A5 (GOLGA5), mRNA.

Price & Availability↑

CloneID	OHu20812
Clone ID Related Accession (Same CDS sequence)	NM_005113.3
Accession Version	NM_005113.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-13
Organism	Homo sapiens(human)
Product	golgin subfamily A member 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB096772.1, AF085199.1, AI288133.1 and AW294222.1. This sequence is a reference standard in the RefSeqGene project. On Jul 23, 2013 this sequence version replaced NM_005113.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.85329.1, SRR1803615.191994.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTCTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GTATTCGCCT GGGAATTTTT CTCCGAAGAT ACCCCATAGC GCGAGTTTTT 
GTAATTATAT ATATGGCTTT GCTTCACCTC TGGGTCATGA TTGTTCTGTT GACTTACACA 
CCAGAAATGC ACCACGACCA ACCATATGGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005104.3
CDS	257..2452
Translation	MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTLQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRYPIARVFVIIYMALLHLWVMIVLLTYTPEMHHDQPYGK

Target ORF information:

RefSeq Version	NM_005113.3
Organism	Homo sapiens(human)
Definition	Homo sapiens golgin A5 (GOLGA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005113.3

ORF Insert Sequence:

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTCTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GTATTCGCCT GGGAATTTTT CTCCGAAGAT ACCCCATAGC GCGAGTTTTT 
GTAATTATAT ATATGGCTTT GCTTCACCTC TGGGTCATGA TTGTTCTGTT GACTTACACA 
CCAGAAATGC ACCACGACCA ACCATATGGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GOLGA5 ( XM_011537420.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535722.1 Homo sapiens golgin A5 (GOLGA5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63546
Clone ID Related Accession (Same CDS sequence)	XM_011537420.2 , XM_011537420.3
Accession Version	XM_011537420.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	golgin subfamily A member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537420.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTTTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GGCTTTGCTT CACCTCTGGG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535722.1
CDS	257..2332
Translation	MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTFQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFRLCFTSGS

Target ORF information:

RefSeq Version	XM_011537420.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgin A5 (GOLGA5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537420.2

ORF Insert Sequence:

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTTTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GGCTTTGCTT CACCTCTGGG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GOLGA5 ( XM_011537420.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535722.1 Homo sapiens golgin A5 (GOLGA5), transcript variant X1, mRNA.

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CloneID	OHu63546
Clone ID Related Accession (Same CDS sequence)	XM_011537420.2 , XM_011537420.3
Accession Version	XM_011537420.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	golgin subfamily A member 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537420.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTTTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GGCTTTGCTT CACCTCTGGG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535722.1
CDS	180..2255
Translation	MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTFQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFRLCFTSGS

Target ORF information:

RefSeq Version	XM_011537420.3
Organism	Homo sapiens(human)
Definition	Homo sapiens golgin A5 (GOLGA5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537420.3

ORF Insert Sequence:

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTTTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GGCTTTGCTT CACCTCTGGG TCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GOLGA5 ( NM_005113.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005104.4 Homo sapiens golgin A5 (GOLGA5), mRNA.

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CloneID	OHu107276
Clone ID Related Accession (Same CDS sequence)	NM_005113.4
Accession Version	NM_005113.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	golgin subfamily A member 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL132987.4. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005113.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.122087.1, SRR1803611.169184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000163416.7/ ENSP00000163416.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTTTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GTATTCGCCT GGGAATTTTT CTCCGAAGAT ACCCCATAGC GCGAGTTTTT 
GTAATTATAT ATATGGCTTT GCTTCACCTC TGGGTCATGA TTGTTCTGTT GACTTACACA 
CCAGAAATGC ACCACGACCA ACCATATGGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005104.4
CDS	183..2378
Translation	MSWFVDLAGKAEDLLNRVDQGAATALSRKDNASNIYSKNTDYTELHQQNTDLIYQTGPKSTYISSAADNIRNQKATILAGTANVKVGSRTPVEASHPVENASVPRPSSHFVRRKKSEPDDELLFDFLNSSQKEPTGRVEIRKEKGKTPVFQSSQTSSVSSVNPSVTTIKTIEENSFGSQTHEAASNSDSSHEGQEESSKENVSSNAACPDHTPTPNDDGKSHELSNLRLENQLLRNEVQSLNQEMASLLQRSKETQEELNKARARVEKWNADHSKSDRMTRGLRAQVDDLTEAVAAKDSQLAVLKVRLQEADQLLSTRTEALEALQSEKSRIMQDQSEGNSLQNQALQTFQERLHEADATLKREQESYKQMQSEFAARLNKVEMERQNLAEAITLAERKYSDEKKRVDELQQQVKLYKLNLESSKQELIDYKQKATRILQSKEKLINSLKEGSGFEGLDSSTASSMELEELRHEKEMQREEIQKLMGQIHQLRSELQDMEAQQVNEAESAREQLQDLHDQIAGQKASKQELETELERLKQEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQLTNKTLSNSSQSELENRLHQLTETLIQKQTMLESLSTEKNSLVFQLERLEQQMNSASGSSSNGSSINMSGIDNGEGTRLRNVPVLFNDTETNLAGMYGKVRKAASSIDQFSIRLGIFLRRYPIARVFVIIYMALLHLWVMIVLLTYTPEMHHDQPYGK

Target ORF information:

RefSeq Version	NM_005113.4
Organism	Homo sapiens(human)
Definition	Homo sapiens golgin A5 (GOLGA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005113.4

ORF Insert Sequence:

ATGTCTTGGT TTGTTGATCT TGCTGGAAAG GCAGAAGATC TTTTAAACCG AGTTGATCAA 
GGGGCTGCAA CAGCTCTCAG TAGGAAAGAC AATGCCAGCA ACATATATAG CAAAAATACT 
GACTATACTG AACTTCACCA GCAAAATACA GATTTGATAT ATCAGACTGG ACCTAAATCT 
ACGTATATTT CATCAGCAGC TGATAACATT CGAAATCAAA AAGCCACCAT CTTAGCTGGC 
ACTGCAAATG TGAAAGTAGG ATCTCGGACA CCAGTAGAGG CCTCTCATCC TGTTGAAAAT 
GCATCTGTTC CTAGGCCTTC ATCCCATTTT GTGCGAAGAA AAAAGTCAGA ACCTGATGAT 
GAGCTGCTGT TTGATTTTCT TAATAGTTCA CAGAAGGAGC CTACCGGGAG GGTGGAAATC 
AGAAAGGAAA AAGGCAAGAC ACCTGTCTTT CAGAGCTCTC AGACATCAAG TGTCAGTTCT 
GTGAACCCCA GTGTAACCAC CATCAAAACC ATTGAAGAAA ATTCTTTTGG GAGCCAAACC 
CACGAAGCTG CCAGTAACTC AGATTCTAGC CATGAAGGTC AAGAGGAATC TTCAAAGGAA 
AATGTGTCAT CAAATGCTGC CTGCCCTGAC CACACCCCAA CACCTAATGA TGATGGCAAA 
TCACATGAAC TGTCTAACCT TCGACTGGAG AATCAGCTGC TGAGGAATGA AGTTCAGTCT 
TTAAATCAAG AAATGGCCTC GTTACTCCAA AGATCCAAAG AGACTCAAGA AGAATTAAAC 
AAAGCAAGAG CAAGAGTTGA AAAGTGGAAT GCTGACCATT CAAAGAGTGA TCGAATGACT 
CGAGGACTCC GAGCCCAAGT AGATGACCTG ACTGAAGCTG TGGCTGCAAA GGATTCCCAG 
CTGGCTGTAC TGAAAGTGAG ACTCCAGGAA GCTGACCAGC TACTGAGTAC TCGCACAGAA 
GCATTAGAAG CCTTACAGAG TGAAAAATCA CGAATAATGC AGGATCAAAG TGAAGGTAAC 
AGCCTGCAGA ATCAAGCTCT GCAGACTTTT CAGGAGAGAC TGCATGAAGC GGATGCCACT 
CTGAAGAGAG AGCAGGAGAG CTATAAACAG ATGCAGAGCG AGTTTGCTGC ACGCCTTAAT 
AAAGTGGAAA TGGAACGTCA GAATTTAGCA GAAGCAATTA CACTGGCCGA AAGAAAATAC 
TCAGATGAGA AGAAGAGGGT TGATGAACTG CAGCAGCAAG TCAAGCTGTA TAAGTTGAAC 
TTGGAGTCCT CTAAGCAGGA ATTAATTGAC TACAAGCAAA AAGCTACTAG AATACTGCAA 
TCTAAGGAAA AATTGATTAA CAGCTTGAAA GAAGGCTCTG GTTTTGAAGG CCTAGATAGC 
AGCACTGCCA GTAGCATGGA GCTGGAAGAA CTTCGGCATG AGAAAGAGAT GCAGAGGGAG 
GAAATACAGA AGCTGATGGG CCAGATACAT CAGCTCAGAT CCGAATTACA GGATATGGAG 
GCACAGCAAG TTAATGAAGC AGAATCAGCA AGAGAACAGT TACAGGATCT GCATGACCAA 
ATAGCTGGGC AGAAAGCATC CAAACAAGAA CTAGAGACAG AACTGGAGCG ACTGAAGCAG 
GAGTTCCACT ATATAGAAGA AGATCTTTAT CGAACAAAGA ACACATTGCA AAGCAGAATT 
AAAGATCGAG ACGAAGAAAT TCAAAAACTC AGGAATCAGC TTACCAATAA AACTTTAAGC 
AATAGCAGTC AGTCTGAGTT AGAAAATCGA CTCCATCAGC TAACAGAGAC TCTCATCCAG 
AAACAGACCA TGCTGGAGAG TCTCAGCACA GAAAAGAACT CCCTGGTCTT TCAACTGGAG 
CGCCTCGAAC AGCAGATGAA CTCCGCCTCT GGAAGTAGTA GTAATGGGTC TTCGATTAAT 
ATGTCTGGAA TTGACAATGG TGAAGGCACT CGTCTGCGAA ATGTTCCTGT TCTTTTTAAT 
GACACAGAAA CTAATCTGGC AGGAATGTAC GGAAAAGTTC GCAAAGCTGC TAGTTCAATT 
GATCAGTTTA GTATTCGCCT GGGAATTTTT CTCCGAAGAT ACCCCATAGC GCGAGTTTTT 
GTAATTATAT ATATGGCTTT GCTTCACCTC TGGGTCATGA TTGTTCTGTT GACTTACACA 
CCAGAAATGC ACCACGACCA ACCATATGGC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Targeting of a chlamydial protease impedes intracellular bacterial growth.
PLoS pathogens7(9)e1002283(2011 Sep)
Christian JG,Heymann J,Paschen SA,Vier J,Schauenburg L,Rupp J,Meyer TF,H?cker G,Heuer D

Interaction of Golgin-84 with the COG complex mediates the intra-Golgi retrograde transport.
Traffic (Copenhagen, Denmark)11(12)1552-66(2010 Dec)
Sohda M,Misumi Y,Yamamoto A,Nakamura N,Ogata S,Sakisaka S,Hirose S,Ikehara Y,Oda K

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction.
Nature457(7230)731-5(2009 Feb)
Heuer D,Rejman Lipinski A,Machuy N,Karlas A,Wehrens A,Siedler F,Brinkmann V,Meyer TF

Golgin tethers define subpopulations of COPI vesicles.
Science (New York, N.Y.)307(5712)1095-8(2005 Feb)
Malsam J,Satoh A,Pelletier L,Warren G

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GOLGA5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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