CDC25B gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_004358.4	NP_004349.1	M-phase inducer phosphatase 2 isoform 2
NM_001287518.1	NP_001274447.1	M-phase inducer phosphatase 2 isoform 6
NM_001287522.1	NP_001274451.1	M-phase inducer phosphatase 2 isoform 8
NM_021872.3	NP_068658.1	M-phase inducer phosphatase 2 isoform 3
NM_001287519.1	NP_001274448.1	M-phase inducer phosphatase 2 isoform 7
NM_021873.3	NP_068659.1	M-phase inducer phosphatase 2 isoform 1
NM_001287517.1	NP_001274446.1	M-phase inducer phosphatase 2 isoform 5
NM_001287524.1	NP_001274453.1	M-phase inducer phosphatase 2 isoform 9
NM_001287516.1	NP_001274445.1	M-phase inducer phosphatase 2 isoform 4
NM_001287520.1	NP_001274449.1	M-phase inducer phosphatase 2 isoform 7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14301	NM_001287524.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu14379	NM_001287520.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu14379	NM_001287519.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu20649	NM_021873.3 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu14266	NM_001287517.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu14272	NM_001287522.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu14285	NM_001287516.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu14337	NM_001287518.1 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu14355	NM_004358.4 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$335.30 $479.00
OHu14356	NM_021872.3 Latest version!	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$321.30 $459.00

CDC25B ( NM_001287524.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274453.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu14301
Clone ID Related Accession (Same CDS sequence)	NM_001287524.1
Accession Version	NM_001287524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 9
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA065196.1, BX640836.1, BC051711.1 and DA107932.1. Transcript Variant: This variant (10) differs in the 5' UTR and has multiple coding region differences, compared to variant 1, which causes translation initiation at a downstream start codon. The resulting protein (isoform 9) has a shorter N-terminus and lacks an internal region compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640836.1, SRR1803616.193084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGATG GATTTGTCTT CAAGATGCCA TGGAAGCCCA CACATCCCAG CTCCACCCAT 
GCTCTGGCAG AGTGGGCCAG CCGCAGGGAA GCCTTTGCCC AGAGACCCAG CTCGGCCCCC 
GACCTGATGT GTCTCAGTCC TGACCGGAAG ATGGAAGTGG AGGAGCTCAG CCCCCTGGCC 
CTAGGTCGCT TCTCTCTGAC CCCTGCAGAG GGGGATACTG AGGAAGATGA TGGATTTGTG 
GACATCCTAG AGAGTGACTT AAAGGATGAT GATGCAGTTC CCCCAGGCAT GGAGAGTCTC 
ATTAGTGCCC CACTGGTCAA GACCTTGGAA AAGGAAGAGG AAAAGGACCT CGTCATGTAC 
AGCAAGTGCC AGCGGCTCTT CCGCTCTCCG TCCATGCCCT GCAGCGTGAT CCGGCCCATC 
CTCAAGAGGC TGGAGCGGCC CCAGGACAGG GACACGCCCG TGCAGAATAA GCGGAGGCGG 
AGCGTGACCC CTCCTGAGGA GCAGCAGGAG GCTGAGGAAC CTAAAGCCCG CGTCCTCCGC 
TCAAAATCAC TGTGTCACGA TGAGATCGAG AACCTCCTGG ACAGTGACCA CCGAGAGCTG 
ATTGGAGATT ACTCTAAGGC CTTCCTCCTA CAGACAGTAG ACGGAAAGCA CCAAGACCTC 
AAGTACATCT CACCAGAAAC GATGGTGGCC CTATTGACGG GCAAGTTCAG CAACATCGTG 
GATAAGTTTG TGATTGTAGA CTGCAGATAC CCCTATGAAT ATGAAGGCGG GCACATCAAG 
ACTGCGGTGA ACTTGCCCCT GGAACGCGAC GCCGAGAGCT TCCTACTGAA GAGCCCCATC 
GCGCCCTGTA GCCTGGACAA GAGAGTCATC CTCATTTTCC ACTGTGAATT CTCATCTGAG 
CGTGGGCCCC GCATGTGCCG TTTCATCAGG GAACGAGACC GTGCTGTCAA CGACTACCCC 
AGCCTCTACT ACCCTGAGAT GTATATCCTG AAAGGCGGCT ACAAGGAGTT CTTCCCTCAG 
CACCCGAACT TCTGTGAACC CCAGGACTAC CGGCCCATGA ACCACGAGGC CTTCAAGGAT 
GAGCTAAAGA CCTTCCGCCT CAAGACTCGC AGCTGGGCTG GGGAGCGGAG CCGGCGGGAG 
CTCTGTAGCC GGCTGCAGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274453.1
CDS	300..1466
Translation	MPDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287524.1

ORF Insert Sequence:

ATGCCGGATG GATTTGTCTT CAAGATGCCA TGGAAGCCCA CACATCCCAG CTCCACCCAT 
GCTCTGGCAG AGTGGGCCAG CCGCAGGGAA GCCTTTGCCC AGAGACCCAG CTCGGCCCCC 
GACCTGATGT GTCTCAGTCC TGACCGGAAG ATGGAAGTGG AGGAGCTCAG CCCCCTGGCC 
CTAGGTCGCT TCTCTCTGAC CCCTGCAGAG GGGGATACTG AGGAAGATGA TGGATTTGTG 
GACATCCTAG AGAGTGACTT AAAGGATGAT GATGCAGTTC CCCCAGGCAT GGAGAGTCTC 
ATTAGTGCCC CACTGGTCAA GACCTTGGAA AAGGAAGAGG AAAAGGACCT CGTCATGTAC 
AGCAAGTGCC AGCGGCTCTT CCGCTCTCCG TCCATGCCCT GCAGCGTGAT CCGGCCCATC 
CTCAAGAGGC TGGAGCGGCC CCAGGACAGG GACACGCCCG TGCAGAATAA GCGGAGGCGG 
AGCGTGACCC CTCCTGAGGA GCAGCAGGAG GCTGAGGAAC CTAAAGCCCG CGTCCTCCGC 
TCAAAATCAC TGTGTCACGA TGAGATCGAG AACCTCCTGG ACAGTGACCA CCGAGAGCTG 
ATTGGAGATT ACTCTAAGGC CTTCCTCCTA CAGACAGTAG ACGGAAAGCA CCAAGACCTC 
AAGTACATCT CACCAGAAAC GATGGTGGCC CTATTGACGG GCAAGTTCAG CAACATCGTG 
GATAAGTTTG TGATTGTAGA CTGCAGATAC CCCTATGAAT ATGAAGGCGG GCACATCAAG 
ACTGCGGTGA ACTTGCCCCT GGAACGCGAC GCCGAGAGCT TCCTACTGAA GAGCCCCATC 
GCGCCCTGTA GCCTGGACAA GAGAGTCATC CTCATTTTCC ACTGTGAATT CTCATCTGAG 
CGTGGGCCCC GCATGTGCCG TTTCATCAGG GAACGAGACC GTGCTGTCAA CGACTACCCC 
AGCCTCTACT ACCCTGAGAT GTATATCCTG AAAGGCGGCT ACAAGGAGTT CTTCCCTCAG 
CACCCGAACT TCTGTGAACC CCAGGACTAC CGGCCCATGA ACCACGAGGC CTTCAAGGAT 
GAGCTAAAGA CCTTCCGCCT CAAGACTCGC AGCTGGGCTG GGGAGCGGAG CCGGCGGGAG 
CTCTGTAGCC GGCTGCAGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_001287519.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274448.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu14379
Clone ID Related Accession (Same CDS sequence)	NM_001287519.1 , NM_001287520.1
Accession Version	NM_001287519.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL109804.41, BX647988.1, BC051711.1, BM793120.1, AL561439.3 and DA107932.1. Transcript Variant: This variant (7) uses an alternate splice site in the 5' UTR which results in initiation of translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 7) has a shorter N-terminus compared to isoform 1. Variants 7 and 8 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.106772.1, SRR1803617.20724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCC CCAGCCCTAT GGACCCCCAC ATGGCGGAGC AGACGTTTGA ACAGGCCATC 
CAGGCAGCCA GCCGGATCAT TCGAAACGAG CAGTTTGCCA TCAGACGCTT CCAGTCTATG 
CCGGTGAGGC TGCTGGGCCA CAGCCCCGTG CTTCGGAACA TCACCAACTC CCAGGCGCCC 
GACGGCCGGA GGAAGAGCGA GGCGGGCAGT GGAGCTGCCA GCAGCTCTGG GGAAGACAAG 
GAGAATGATG GATTTGTCTT CAAGATGCCA TGGAAGCCCA CACATCCCAG CTCCACCCAT 
GCTCTGGCAG AGTGGGCCAG CCGCAGGGAA GCCTTTGCCC AGAGACCCAG CTCGGCCCCC 
GACCTGATGT GTCTCAGTCC TGACCGGAAG ATGGAAGTGG AGGAGCTCAG CCCCCTGGCC 
CTAGGTCGCT TCTCTCTGAC CCCTGCAGAG GGGGATACTG AGGAAGATGA TGGATTTGTG 
GACATCCTAG AGAGTGACTT AAAGGATGAT GATGCAGTTC CCCCAGGCAT GGAGAGTCTC 
ATTAGTGCCC CACTGGTCAA GACCTTGGAA AAGGAAGAGG AAAAGGACCT CGTCATGTAC 
AGCAAGTGCC AGCGGCTCTT CCGCTCTCCG TCCATGCCCT GCAGCGTGAT CCGGCCCATC 
CTCAAGAGGC TGGAGCGGCC CCAGGACAGG GACACGCCCG TGCAGAATAA GCGGAGGCGG 
AGCGTGACCC CTCCTGAGGA GCAGCAGGAG GCTGAGGAAC CTAAAGCCCG CGTCCTCCGC 
TCAAAATCAC TGTGTCACGA TGAGATCGAG AACCTCCTGG ACAGTGACCA CCGAGAGCTG 
ATTGGAGATT ACTCTAAGGC CTTCCTCCTA CAGACAGTAG ACGGAAAGCA CCAAGACCTC 
AAGTACATCT CACCAGAAAC GATGGTGGCC CTATTGACGG GCAAGTTCAG CAACATCGTG 
GATAAGTTTG TGATTGTAGA CTGCAGATAC CCCTATGAAT ATGAAGGCGG GCACATCAAG 
ACTGCGGTGA ACTTGCCCCT GGAACGCGAC GCCGAGAGCT TCCTACTGAA GAGCCCCATC 
GCGCCCTGTA GCCTGGACAA GAGAGTCATC CTCATTTTCC ACTGTGAATT CTCATCTGAG 
CGTGGGCCCC GCATGTGCCG TTTCATCAGG GAACGAGACC GTGCTGTCAA CGACTACCCC 
AGCCTCTACT ACCCTGAGAT GTATATCCTG AAAGGCGGCT ACAAGGAGTT CTTCCCTCAG 
CACCCGAACT TCTGTGAACC CCAGGACTAC CGGCCCATGA ACCACGAGGC CTTCAAGGAT 
GAGCTAAAGA CCTTCCGCCT CAAGACTCGC AGCTGGGCTG GGGAGCGGAG CCGGCGGGAG 
CTCTGTAGCC GGCTGCAGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274448.1
CDS	236..1642
Translation	MDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287519.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287519.1

ORF Insert Sequence:

ATGGATTCCC CCAGCCCTAT GGACCCCCAC ATGGCGGAGC AGACGTTTGA ACAGGCCATC 
CAGGCAGCCA GCCGGATCAT TCGAAACGAG CAGTTTGCCA TCAGACGCTT CCAGTCTATG 
CCGGTGAGGC TGCTGGGCCA CAGCCCCGTG CTTCGGAACA TCACCAACTC CCAGGCGCCC 
GACGGCCGGA GGAAGAGCGA GGCGGGCAGT GGAGCTGCCA GCAGCTCTGG GGAAGACAAG 
GAGAATGATG GATTTGTCTT CAAGATGCCA TGGAAGCCCA CACATCCCAG CTCCACCCAT 
GCTCTGGCAG AGTGGGCCAG CCGCAGGGAA GCCTTTGCCC AGAGACCCAG CTCGGCCCCC 
GACCTGATGT GTCTCAGTCC TGACCGGAAG ATGGAAGTGG AGGAGCTCAG CCCCCTGGCC 
CTAGGTCGCT TCTCTCTGAC CCCTGCAGAG GGGGATACTG AGGAAGATGA TGGATTTGTG 
GACATCCTAG AGAGTGACTT AAAGGATGAT GATGCAGTTC CCCCAGGCAT GGAGAGTCTC 
ATTAGTGCCC CACTGGTCAA GACCTTGGAA AAGGAAGAGG AAAAGGACCT CGTCATGTAC 
AGCAAGTGCC AGCGGCTCTT CCGCTCTCCG TCCATGCCCT GCAGCGTGAT CCGGCCCATC 
CTCAAGAGGC TGGAGCGGCC CCAGGACAGG GACACGCCCG TGCAGAATAA GCGGAGGCGG 
AGCGTGACCC CTCCTGAGGA GCAGCAGGAG GCTGAGGAAC CTAAAGCCCG CGTCCTCCGC 
TCAAAATCAC TGTGTCACGA TGAGATCGAG AACCTCCTGG ACAGTGACCA CCGAGAGCTG 
ATTGGAGATT ACTCTAAGGC CTTCCTCCTA CAGACAGTAG ACGGAAAGCA CCAAGACCTC 
AAGTACATCT CACCAGAAAC GATGGTGGCC CTATTGACGG GCAAGTTCAG CAACATCGTG 
GATAAGTTTG TGATTGTAGA CTGCAGATAC CCCTATGAAT ATGAAGGCGG GCACATCAAG 
ACTGCGGTGA ACTTGCCCCT GGAACGCGAC GCCGAGAGCT TCCTACTGAA GAGCCCCATC 
GCGCCCTGTA GCCTGGACAA GAGAGTCATC CTCATTTTCC ACTGTGAATT CTCATCTGAG 
CGTGGGCCCC GCATGTGCCG TTTCATCAGG GAACGAGACC GTGCTGTCAA CGACTACCCC 
AGCCTCTACT ACCCTGAGAT GTATATCCTG AAAGGCGGCT ACAAGGAGTT CTTCCCTCAG 
CACCCGAACT TCTGTGAACC CCAGGACTAC CGGCCCATGA ACCACGAGGC CTTCAAGGAT 
GAGCTAAAGA CCTTCCGCCT CAAGACTCGC AGCTGGGCTG GGGAGCGGAG CCGGCGGGAG 
CTCTGTAGCC GGCTGCAGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_001287520.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274449.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu14379
Clone ID Related Accession (Same CDS sequence)	NM_001287519.1 , NM_001287520.1
Accession Version	NM_001287520.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FR695900.1 and DA107932.1. On Dec 17, 2013 this sequence version replaced XM_005260900.1. Transcript Variant: This variant (8), also known as CDC25B6, uses an alternate splice site in the 5' UTR which results in initiation of translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 7) has a shorter N-terminus compared to isoform 1. Variants 7 and 8 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FR695900.1, SRR1803613.62917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCC CCAGCCCTAT GGACCCCCAC ATGGCGGAGC AGACGTTTGA ACAGGCCATC 
CAGGCAGCCA GCCGGATCAT TCGAAACGAG CAGTTTGCCA TCAGACGCTT CCAGTCTATG 
CCGGTGAGGC TGCTGGGCCA CAGCCCCGTG CTTCGGAACA TCACCAACTC CCAGGCGCCC 
GACGGCCGGA GGAAGAGCGA GGCGGGCAGT GGAGCTGCCA GCAGCTCTGG GGAAGACAAG 
GAGAATGATG GATTTGTCTT CAAGATGCCA TGGAAGCCCA CACATCCCAG CTCCACCCAT 
GCTCTGGCAG AGTGGGCCAG CCGCAGGGAA GCCTTTGCCC AGAGACCCAG CTCGGCCCCC 
GACCTGATGT GTCTCAGTCC TGACCGGAAG ATGGAAGTGG AGGAGCTCAG CCCCCTGGCC 
CTAGGTCGCT TCTCTCTGAC CCCTGCAGAG GGGGATACTG AGGAAGATGA TGGATTTGTG 
GACATCCTAG AGAGTGACTT AAAGGATGAT GATGCAGTTC CCCCAGGCAT GGAGAGTCTC 
ATTAGTGCCC CACTGGTCAA GACCTTGGAA AAGGAAGAGG AAAAGGACCT CGTCATGTAC 
AGCAAGTGCC AGCGGCTCTT CCGCTCTCCG TCCATGCCCT GCAGCGTGAT CCGGCCCATC 
CTCAAGAGGC TGGAGCGGCC CCAGGACAGG GACACGCCCG TGCAGAATAA GCGGAGGCGG 
AGCGTGACCC CTCCTGAGGA GCAGCAGGAG GCTGAGGAAC CTAAAGCCCG CGTCCTCCGC 
TCAAAATCAC TGTGTCACGA TGAGATCGAG AACCTCCTGG ACAGTGACCA CCGAGAGCTG 
ATTGGAGATT ACTCTAAGGC CTTCCTCCTA CAGACAGTAG ACGGAAAGCA CCAAGACCTC 
AAGTACATCT CACCAGAAAC GATGGTGGCC CTATTGACGG GCAAGTTCAG CAACATCGTG 
GATAAGTTTG TGATTGTAGA CTGCAGATAC CCCTATGAAT ATGAAGGCGG GCACATCAAG 
ACTGCGGTGA ACTTGCCCCT GGAACGCGAC GCCGAGAGCT TCCTACTGAA GAGCCCCATC 
GCGCCCTGTA GCCTGGACAA GAGAGTCATC CTCATTTTCC ACTGTGAATT CTCATCTGAG 
CGTGGGCCCC GCATGTGCCG TTTCATCAGG GAACGAGACC GTGCTGTCAA CGACTACCCC 
AGCCTCTACT ACCCTGAGAT GTATATCCTG AAAGGCGGCT ACAAGGAGTT CTTCCCTCAG 
CACCCGAACT TCTGTGAACC CCAGGACTAC CGGCCCATGA ACCACGAGGC CTTCAAGGAT 
GAGCTAAAGA CCTTCCGCCT CAAGACTCGC AGCTGGGCTG GGGAGCGGAG CCGGCGGGAG 
CTCTGTAGCC GGCTGCAGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274449.1
CDS	278..1684
Translation	MDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287520.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287520.1

ORF Insert Sequence:

ATGGATTCCC CCAGCCCTAT GGACCCCCAC ATGGCGGAGC AGACGTTTGA ACAGGCCATC 
CAGGCAGCCA GCCGGATCAT TCGAAACGAG CAGTTTGCCA TCAGACGCTT CCAGTCTATG 
CCGGTGAGGC TGCTGGGCCA CAGCCCCGTG CTTCGGAACA TCACCAACTC CCAGGCGCCC 
GACGGCCGGA GGAAGAGCGA GGCGGGCAGT GGAGCTGCCA GCAGCTCTGG GGAAGACAAG 
GAGAATGATG GATTTGTCTT CAAGATGCCA TGGAAGCCCA CACATCCCAG CTCCACCCAT 
GCTCTGGCAG AGTGGGCCAG CCGCAGGGAA GCCTTTGCCC AGAGACCCAG CTCGGCCCCC 
GACCTGATGT GTCTCAGTCC TGACCGGAAG ATGGAAGTGG AGGAGCTCAG CCCCCTGGCC 
CTAGGTCGCT TCTCTCTGAC CCCTGCAGAG GGGGATACTG AGGAAGATGA TGGATTTGTG 
GACATCCTAG AGAGTGACTT AAAGGATGAT GATGCAGTTC CCCCAGGCAT GGAGAGTCTC 
ATTAGTGCCC CACTGGTCAA GACCTTGGAA AAGGAAGAGG AAAAGGACCT CGTCATGTAC 
AGCAAGTGCC AGCGGCTCTT CCGCTCTCCG TCCATGCCCT GCAGCGTGAT CCGGCCCATC 
CTCAAGAGGC TGGAGCGGCC CCAGGACAGG GACACGCCCG TGCAGAATAA GCGGAGGCGG 
AGCGTGACCC CTCCTGAGGA GCAGCAGGAG GCTGAGGAAC CTAAAGCCCG CGTCCTCCGC 
TCAAAATCAC TGTGTCACGA TGAGATCGAG AACCTCCTGG ACAGTGACCA CCGAGAGCTG 
ATTGGAGATT ACTCTAAGGC CTTCCTCCTA CAGACAGTAG ACGGAAAGCA CCAAGACCTC 
AAGTACATCT CACCAGAAAC GATGGTGGCC CTATTGACGG GCAAGTTCAG CAACATCGTG 
GATAAGTTTG TGATTGTAGA CTGCAGATAC CCCTATGAAT ATGAAGGCGG GCACATCAAG 
ACTGCGGTGA ACTTGCCCCT GGAACGCGAC GCCGAGAGCT TCCTACTGAA GAGCCCCATC 
GCGCCCTGTA GCCTGGACAA GAGAGTCATC CTCATTTTCC ACTGTGAATT CTCATCTGAG 
CGTGGGCCCC GCATGTGCCG TTTCATCAGG GAACGAGACC GTGCTGTCAA CGACTACCCC 
AGCCTCTACT ACCCTGAGAT GTATATCCTG AAAGGCGGCT ACAAGGAGTT CTTCCCTCAG 
CACCCGAACT TCTGTGAACC CCAGGACTAC CGGCCCATGA ACCACGAGGC CTTCAAGGAT 
GAGCTAAAGA CCTTCCGCCT CAAGACTCGC AGCTGGGCTG GGGAGCGGAG CCGGCGGGAG 
CTCTGTAGCC GGCTGCAGGA CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_021873.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068659.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 1, mRNA.

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CloneID	OHu20649
Clone ID Related Accession (Same CDS sequence)	NM_021873.3
Accession Version	NM_021873.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092713.1, BC051711.1, S78187.1, BX640836.1 and DA107932.1. This sequence is a reference standard in the RefSeqGene project. On Dec 17, 2013 this sequence version replaced NM_021873.2. Transcript Variant: This variant (1), also known as CDC25B3, represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC051711.1, SRR1660809.58539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGC CCCAGCCGGA GCCCGCGCCA GGCTCGGCTC TCAGTCCAGC AGGCGTGTGC 
GGTGGCGCCC AGCGTCCGGG CCACCTCCCG GGCCTCCTGC TGGGATCTCA TGGCCTCCTG 
GGGTCCCCGG TGCGGGCGGC CGCTTCCTCG CCGGTCACCA CCCTCACCCA GACCATGCAC 
GACCTCGCCG GGCTCGGCAG CGAAACCCCA AAGAGTCAGG TAGGGACCCT GCTCTTCCGC 
AGCCGCAGCC GCCTGACGCA CCTATCCCTG TCTCGACGGG CATCCGAATC CTCCCTGTCG 
TCTGAATCCT CCGAATCTTC TGATGCAGGT CTCTGCATGG ATTCCCCCAG CCCTATGGAC 
CCCCACATGG CGGAGCAGAC GTTTGAACAG GCCATCCAGG CAGCCAGCCG GATCATTCGA 
AACGAGCAGT TTGCCATCAG ACGCTTCCAG TCTATGCCGG TGAGGCTGCT GGGCCACAGC 
CCCGTGCTTC GGAACATCAC CAACTCCCAG GCGCCCGACG GCCGGAGGAA GAGCGAGGCG 
GGCAGTGGAG CTGCCAGCAG CTCTGGGGAA GACAAGGAGA ATGATGGATT TGTCTTCAAG 
ATGCCATGGA AGCCCACACA TCCCAGCTCC ACCCATGCTC TGGCAGAGTG GGCCAGCCGC 
AGGGAAGCCT TTGCCCAGAG ACCCAGCTCG GCCCCCGACC TGATGTGTCT CAGTCCTGAC 
CGGAAGATGG AAGTGGAGGA GCTCAGCCCC CTGGCCCTAG GTCGCTTCTC TCTGACCCCT 
GCAGAGGGGG ATACTGAGGA AGATGATGGA TTTGTGGACA TCCTAGAGAG TGACTTAAAG 
GATGATGATG CAGTTCCCCC AGGCATGGAG AGTCTCATTA GTGCCCCACT GGTCAAGACC 
TTGGAAAAGG AAGAGGAAAA GGACCTCGTC ATGTACAGCA AGTGCCAGCG GCTCTTCCGC 
TCTCCGTCCA TGCCCTGCAG CGTGATCCGG CCCATCCTCA AGAGGCTGGA GCGGCCCCAG 
GACAGGGACA CGCCCGTGCA GAATAAGCGG AGGCGGAGCG TGACCCCTCC TGAGGAGCAG 
CAGGAGGCTG AGGAACCTAA AGCCCGCGTC CTCCGCTCAA AATCACTGTG TCACGATGAG 
ATCGAGAACC TCCTGGACAG TGACCACCGA GAGCTGATTG GAGATTACTC TAAGGCCTTC 
CTCCTACAGA CAGTAGACGG AAAGCACCAA GACCTCAAGT ACATCTCACC AGAAACGATG 
GTGGCCCTAT TGACGGGCAA GTTCAGCAAC ATCGTGGATA AGTTTGTGAT TGTAGACTGC 
AGATACCCCT ATGAATATGA AGGCGGGCAC ATCAAGACTG CGGTGAACTT GCCCCTGGAA 
CGCGACGCCG AGAGCTTCCT ACTGAAGAGC CCCATCGCGC CCTGTAGCCT GGACAAGAGA 
GTCATCCTCA TTTTCCACTG TGAATTCTCA TCTGAGCGTG GGCCCCGCAT GTGCCGTTTC 
ATCAGGGAAC GAGACCGTGC TGTCAACGAC TACCCCAGCC TCTACTACCC TGAGATGTAT 
ATCCTGAAAG GCGGCTACAA GGAGTTCTTC CCTCAGCACC CGAACTTCTG TGAACCCCAG 
GACTACCGGC CCATGAACCA CGAGGCCTTC AAGGATGAGC TAAAGACCTT CCGCCTCAAG 
ACTCGCAGCT GGGCTGGGGA GCGGAGCCGG CGGGAGCTCT GTAGCCGGCT GCAGGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068659.1
CDS	794..2536
Translation	MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_021873.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021873.3

ORF Insert Sequence:

ATGGAGGTGC CCCAGCCGGA GCCCGCGCCA GGCTCGGCTC TCAGTCCAGC AGGCGTGTGC 
GGTGGCGCCC AGCGTCCGGG CCACCTCCCG GGCCTCCTGC TGGGATCTCA TGGCCTCCTG 
GGGTCCCCGG TGCGGGCGGC CGCTTCCTCG CCGGTCACCA CCCTCACCCA GACCATGCAC 
GACCTCGCCG GGCTCGGCAG CGAAACCCCA AAGAGTCAGG TAGGGACCCT GCTCTTCCGC 
AGCCGCAGCC GCCTGACGCA CCTATCCCTG TCTCGACGGG CATCCGAATC CTCCCTGTCG 
TCTGAATCCT CCGAATCTTC TGATGCAGGT CTCTGCATGG ATTCCCCCAG CCCTATGGAC 
CCCCACATGG CGGAGCAGAC GTTTGAACAG GCCATCCAGG CAGCCAGCCG GATCATTCGA 
AACGAGCAGT TTGCCATCAG ACGCTTCCAG TCTATGCCGG TGAGGCTGCT GGGCCACAGC 
CCCGTGCTTC GGAACATCAC CAACTCCCAG GCGCCCGACG GCCGGAGGAA GAGCGAGGCG 
GGCAGTGGAG CTGCCAGCAG CTCTGGGGAA GACAAGGAGA ATGATGGATT TGTCTTCAAG 
ATGCCATGGA AGCCCACACA TCCCAGCTCC ACCCATGCTC TGGCAGAGTG GGCCAGCCGC 
AGGGAAGCCT TTGCCCAGAG ACCCAGCTCG GCCCCCGACC TGATGTGTCT CAGTCCTGAC 
CGGAAGATGG AAGTGGAGGA GCTCAGCCCC CTGGCCCTAG GTCGCTTCTC TCTGACCCCT 
GCAGAGGGGG ATACTGAGGA AGATGATGGA TTTGTGGACA TCCTAGAGAG TGACTTAAAG 
GATGATGATG CAGTTCCCCC AGGCATGGAG AGTCTCATTA GTGCCCCACT GGTCAAGACC 
TTGGAAAAGG AAGAGGAAAA GGACCTCGTC ATGTACAGCA AGTGCCAGCG GCTCTTCCGC 
TCTCCGTCCA TGCCCTGCAG CGTGATCCGG CCCATCCTCA AGAGGCTGGA GCGGCCCCAG 
GACAGGGACA CGCCCGTGCA GAATAAGCGG AGGCGGAGCG TGACCCCTCC TGAGGAGCAG 
CAGGAGGCTG AGGAACCTAA AGCCCGCGTC CTCCGCTCAA AATCACTGTG TCACGATGAG 
ATCGAGAACC TCCTGGACAG TGACCACCGA GAGCTGATTG GAGATTACTC TAAGGCCTTC 
CTCCTACAGA CAGTAGACGG AAAGCACCAA GACCTCAAGT ACATCTCACC AGAAACGATG 
GTGGCCCTAT TGACGGGCAA GTTCAGCAAC ATCGTGGATA AGTTTGTGAT TGTAGACTGC 
AGATACCCCT ATGAATATGA AGGCGGGCAC ATCAAGACTG CGGTGAACTT GCCCCTGGAA 
CGCGACGCCG AGAGCTTCCT ACTGAAGAGC CCCATCGCGC CCTGTAGCCT GGACAAGAGA 
GTCATCCTCA TTTTCCACTG TGAATTCTCA TCTGAGCGTG GGCCCCGCAT GTGCCGTTTC 
ATCAGGGAAC GAGACCGTGC TGTCAACGAC TACCCCAGCC TCTACTACCC TGAGATGTAT 
ATCCTGAAAG GCGGCTACAA GGAGTTCTTC CCTCAGCACC CGAACTTCTG TGAACCCCAG 
GACTACCGGC CCATGAACCA CGAGGCCTTC AAGGATGAGC TAAAGACCTT CCGCCTCAAG 
ACTCGCAGCT GGGCTGGGGA GCGGAGCCGG CGGGAGCTCT GTAGCCGGCT GCAGGACCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_001287517.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274446.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 5, mRNA.

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CloneID	OHu14266
Clone ID Related Accession (Same CDS sequence)	NM_001287517.1
Accession Version	NM_001287517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1509bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB477384.1, AK299192.1, AL109804.41 and DA107932.1. On Dec 17, 2013 this sequence version replaced XM_005260898.1. Transcript Variant: This variant (5) differs in the 5' UTR which results in initiation of translation at an upstream start codon, and uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The resulting protein (isoform 5) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.224928.1, SRR1660805.70783.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGCC GCAGCCGCCT GACGCACCTA TCCCTGTCTC GACGGGCATC CGAATCCTCC 
CTGTCGTCTG AATCCTCCGA ATCTTCTGAT GCAGGTCTCT GCATGGATTC CCCCAGCCCT 
ATGGACCCCC ACATGGCGGA GCAGACGTTT GAACAGGCCA TCCAGGCAGC CAGCCGGATC 
ATTCGAAACG AGCAGTTTGC CATCAGACGC TTCCAGTCTA TGCCGGTGAG GCTGCTGGGC 
CACAGCCCCG TGCTTCGGAA CATCACCAAC TCCCAGGCGC CCGACGGCCG GAGGAAGAGC 
GAGGCGGGCA GTGGAGCTGC CAGCAGCTCT GGGGAAGACA AGGAGAATGA TGGATTTGTC 
TTCAAGATGC CATGGAAGCC CACACATCCC AGCTCCACCC ATGCTCTGGC AGAGTGGGCC 
AGCCGCAGGG AAGCCTTTGC CCAGAGACCC AGCTCGGCCC CCGACCTGAT GTGTCTCAGT 
CCTGACCGGA AGATGGAAGT GGAGGAGCTC AGCCCCCTGG CCCTAGGTCG CTTCTCTCTG 
ACCCCTGCAG AGGGGGATAC TGAGGAAGAT GATGGATTTG TGGACATCCT AGAGAGTGAC 
TTAAAGGATG ATGATGCAGT TCCCCCAGGC ATGGAGAGTC TCATTAGTGC CCCACTGGTC 
AAGACCTTGG AAAAGGAAGA GGAAAAGGAC CTCGTCATGT ACAGCAAGTG CCAGCGGCTC 
TTCCGCTCTC CGTCCATGCC CTGCAGCGTG ATCCGGCCCA TCCTCAAGAG GCTGGAGCGG 
CCCCAGGACA GGGACACGCC CGTGCAGAAT AAGCGGAGGC GGAGCGTGAC CCCTCCTGAG 
GAGCAGCAGG AGGCTGAGGA ACCTAAAGCC CGCGTCCTCC GCTCAAAATC ACTGTGTCAC 
GATGAGATCG AGAACCTCCT GGACAGTGAC CACCGAGAGC TGATTGGAGA TTACTCTAAG 
GCCTTCCTCC TACAGACAGT AGACGGAAAG CACCAAGACC TCAAGTACAT CTCACCAGAA 
ACGATGGTGG CCCTATTGAC GGGCAAGTTC AGCAACATCG TGGATAAGTT TGTGATTGTA 
GACTGCAGAT ACCCCTATGA ATATGAAGGC GGGCACATCA AGACTGCGGT GAACTTGCCC 
CTGGAACGCG ACGCCGAGAG CTTCCTACTG AAGAGCCCCA TCGCGCCCTG TAGCCTGGAC 
AAGAGAGTCA TCCTCATTTT CCACTGTGAA TTCTCATCTG AGCGTGGGCC CCGCATGTGC 
CGTTTCATCA GGGAACGAGA CCGTGCTGTC AACGACTACC CCAGCCTCTA CTACCCTGAG 
ATGTATATCC TGAAAGGCGG CTACAAGGAG TTCTTCCCTC AGCACCCGAA CTTCTGTGAA 
CCCCAGGACT ACCGGCCCAT GAACCACGAG GCCTTCAAGG ATGAGCTAAA GACCTTCCGC 
CTCAAGACTC GCAGCTGGGC TGGGGAGCGG AGCCGGCGGG AGCTCTGTAG CCGGCTGCAG 
GACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274446.1
CDS	361..1869
Translation	MHSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287517.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287517.1

ORF Insert Sequence:

ATGCACAGCC GCAGCCGCCT GACGCACCTA TCCCTGTCTC GACGGGCATC CGAATCCTCC 
CTGTCGTCTG AATCCTCCGA ATCTTCTGAT GCAGGTCTCT GCATGGATTC CCCCAGCCCT 
ATGGACCCCC ACATGGCGGA GCAGACGTTT GAACAGGCCA TCCAGGCAGC CAGCCGGATC 
ATTCGAAACG AGCAGTTTGC CATCAGACGC TTCCAGTCTA TGCCGGTGAG GCTGCTGGGC 
CACAGCCCCG TGCTTCGGAA CATCACCAAC TCCCAGGCGC CCGACGGCCG GAGGAAGAGC 
GAGGCGGGCA GTGGAGCTGC CAGCAGCTCT GGGGAAGACA AGGAGAATGA TGGATTTGTC 
TTCAAGATGC CATGGAAGCC CACACATCCC AGCTCCACCC ATGCTCTGGC AGAGTGGGCC 
AGCCGCAGGG AAGCCTTTGC CCAGAGACCC AGCTCGGCCC CCGACCTGAT GTGTCTCAGT 
CCTGACCGGA AGATGGAAGT GGAGGAGCTC AGCCCCCTGG CCCTAGGTCG CTTCTCTCTG 
ACCCCTGCAG AGGGGGATAC TGAGGAAGAT GATGGATTTG TGGACATCCT AGAGAGTGAC 
TTAAAGGATG ATGATGCAGT TCCCCCAGGC ATGGAGAGTC TCATTAGTGC CCCACTGGTC 
AAGACCTTGG AAAAGGAAGA GGAAAAGGAC CTCGTCATGT ACAGCAAGTG CCAGCGGCTC 
TTCCGCTCTC CGTCCATGCC CTGCAGCGTG ATCCGGCCCA TCCTCAAGAG GCTGGAGCGG 
CCCCAGGACA GGGACACGCC CGTGCAGAAT AAGCGGAGGC GGAGCGTGAC CCCTCCTGAG 
GAGCAGCAGG AGGCTGAGGA ACCTAAAGCC CGCGTCCTCC GCTCAAAATC ACTGTGTCAC 
GATGAGATCG AGAACCTCCT GGACAGTGAC CACCGAGAGC TGATTGGAGA TTACTCTAAG 
GCCTTCCTCC TACAGACAGT AGACGGAAAG CACCAAGACC TCAAGTACAT CTCACCAGAA 
ACGATGGTGG CCCTATTGAC GGGCAAGTTC AGCAACATCG TGGATAAGTT TGTGATTGTA 
GACTGCAGAT ACCCCTATGA ATATGAAGGC GGGCACATCA AGACTGCGGT GAACTTGCCC 
CTGGAACGCG ACGCCGAGAG CTTCCTACTG AAGAGCCCCA TCGCGCCCTG TAGCCTGGAC 
AAGAGAGTCA TCCTCATTTT CCACTGTGAA TTCTCATCTG AGCGTGGGCC CCGCATGTGC 
CGTTTCATCA GGGAACGAGA CCGTGCTGTC AACGACTACC CCAGCCTCTA CTACCCTGAG 
ATGTATATCC TGAAAGGCGG CTACAAGGAG TTCTTCCCTC AGCACCCGAA CTTCTGTGAA 
CCCCAGGACT ACCGGCCCAT GAACCACGAG GCCTTCAAGG ATGAGCTAAA GACCTTCCGC 
CTCAAGACTC GCAGCTGGGC TGGGGAGCGG AGCCGGCGGG AGCTCTGTAG CCGGCTGCAG 
GACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_001287522.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274451.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 9, mRNA.

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CloneID	OHu14272
Clone ID Related Accession (Same CDS sequence)	NM_001287522.1
Accession Version	NM_001287522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1284bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FR695901.1, AL561439.3 and DA107932.1. Transcript Variant: This variant (9) uses an alternate splice site in the 5' UTR which results in initiation of translation at a downstream start codon, compared to variant 1. The encoded protein (isoform 8) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FR695901.1, SRR1803616.79733.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCCC CCAGCCCTAT GGACCCCCAC ATGGCGGAGC AGACGTTTGA ACAGGCCATC 
CAGGCAGCCA GCCGGATCAT TCGAAACGAG CAGTTTGCCA TCAGACGCTT CCAGTCTATG 
CCGGATGGAT TTGTCTTCAA GATGCCATGG AAGCCCACAC ATCCCAGCTC CACCCATGCT 
CTGGCAGAGT GGGCCAGCCG CAGGGAAGCC TTTGCCCAGA GACCCAGCTC GGCCCCCGAC 
CTGATGTGTC TCAGTCCTGA CCGGAAGATG GAAGTGGAGG AGCTCAGCCC CCTGGCCCTA 
GGTCGCTTCT CTCTGACCCC TGCAGAGGGG GATACTGAGG AAGATGATGG ATTTGTGGAC 
ATCCTAGAGA GTGACTTAAA GGATGATGAT GCAGTTCCCC CAGGCATGGA GAGTCTCATT 
AGTGCCCCAC TGGTCAAGAC CTTGGAAAAG GAAGAGGAAA AGGACCTCGT CATGTACAGC 
AAGTGCCAGC GGCTCTTCCG CTCTCCGTCC ATGCCCTGCA GCGTGATCCG GCCCATCCTC 
AAGAGGCTGG AGCGGCCCCA GGACAGGGAC ACGCCCGTGC AGAATAAGCG GAGGCGGAGC 
GTGACCCCTC CTGAGGAGCA GCAGGAGGCT GAGGAACCTA AAGCCCGCGT CCTCCGCTCA 
AAATCACTGT GTCACGATGA GATCGAGAAC CTCCTGGACA GTGACCACCG AGAGCTGATT 
GGAGATTACT CTAAGGCCTT CCTCCTACAG ACAGTAGACG GAAAGCACCA AGACCTCAAG 
TACATCTCAC CAGAAACGAT GGTGGCCCTA TTGACGGGCA AGTTCAGCAA CATCGTGGAT 
AAGTTTGTGA TTGTAGACTG CAGATACCCC TATGAATATG AAGGCGGGCA CATCAAGACT 
GCGGTGAACT TGCCCCTGGA ACGCGACGCC GAGAGCTTCC TACTGAAGAG CCCCATCGCG 
CCCTGTAGCC TGGACAAGAG AGTCATCCTC ATTTTCCACT GTGAATTCTC ATCTGAGCGT 
GGGCCCCGCA TGTGCCGTTT CATCAGGGAA CGAGACCGTG CTGTCAACGA CTACCCCAGC 
CTCTACTACC CTGAGATGTA TATCCTGAAA GGCGGCTACA AGGAGTTCTT CCCTCAGCAC 
CCGAACTTCT GTGAACCCCA GGACTACCGG CCCATGAACC ACGAGGCCTT CAAGGATGAG 
CTAAAGACCT TCCGCCTCAA GACTCGCAGC TGGGCTGGGG AGCGGAGCCG GCGGGAGCTC 
TGTAGCCGGC TGCAGGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274451.1
CDS	278..1561
Translation	MDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287522.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287522.1

ORF Insert Sequence:

ATGGATTCCC CCAGCCCTAT GGACCCCCAC ATGGCGGAGC AGACGTTTGA ACAGGCCATC 
CAGGCAGCCA GCCGGATCAT TCGAAACGAG CAGTTTGCCA TCAGACGCTT CCAGTCTATG 
CCGGATGGAT TTGTCTTCAA GATGCCATGG AAGCCCACAC ATCCCAGCTC CACCCATGCT 
CTGGCAGAGT GGGCCAGCCG CAGGGAAGCC TTTGCCCAGA GACCCAGCTC GGCCCCCGAC 
CTGATGTGTC TCAGTCCTGA CCGGAAGATG GAAGTGGAGG AGCTCAGCCC CCTGGCCCTA 
GGTCGCTTCT CTCTGACCCC TGCAGAGGGG GATACTGAGG AAGATGATGG ATTTGTGGAC 
ATCCTAGAGA GTGACTTAAA GGATGATGAT GCAGTTCCCC CAGGCATGGA GAGTCTCATT 
AGTGCCCCAC TGGTCAAGAC CTTGGAAAAG GAAGAGGAAA AGGACCTCGT CATGTACAGC 
AAGTGCCAGC GGCTCTTCCG CTCTCCGTCC ATGCCCTGCA GCGTGATCCG GCCCATCCTC 
AAGAGGCTGG AGCGGCCCCA GGACAGGGAC ACGCCCGTGC AGAATAAGCG GAGGCGGAGC 
GTGACCCCTC CTGAGGAGCA GCAGGAGGCT GAGGAACCTA AAGCCCGCGT CCTCCGCTCA 
AAATCACTGT GTCACGATGA GATCGAGAAC CTCCTGGACA GTGACCACCG AGAGCTGATT 
GGAGATTACT CTAAGGCCTT CCTCCTACAG ACAGTAGACG GAAAGCACCA AGACCTCAAG 
TACATCTCAC CAGAAACGAT GGTGGCCCTA TTGACGGGCA AGTTCAGCAA CATCGTGGAT 
AAGTTTGTGA TTGTAGACTG CAGATACCCC TATGAATATG AAGGCGGGCA CATCAAGACT 
GCGGTGAACT TGCCCCTGGA ACGCGACGCC GAGAGCTTCC TACTGAAGAG CCCCATCGCG 
CCCTGTAGCC TGGACAAGAG AGTCATCCTC ATTTTCCACT GTGAATTCTC ATCTGAGCGT 
GGGCCCCGCA TGTGCCGTTT CATCAGGGAA CGAGACCGTG CTGTCAACGA CTACCCCAGC 
CTCTACTACC CTGAGATGTA TATCCTGAAA GGCGGCTACA AGGAGTTCTT CCCTCAGCAC 
CCGAACTTCT GTGAACCCCA GGACTACCGG CCCATGAACC ACGAGGCCTT CAAGGATGAG 
CTAAAGACCT TCCGCCTCAA GACTCGCAGC TGGGCTGGGG AGCGGAGCCG GCGGGAGCTC 
TGTAGCCGGC TGCAGGACCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_001287516.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274445.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu14285
Clone ID Related Accession (Same CDS sequence)	NM_001287516.1
Accession Version	NM_001287516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1551bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB477384.1, AK295573.1, AL109804.41 and DA107932.1. On Dec 17, 2013 this sequence version replaced XM_005260897.1. Transcript Variant: This variant (4) differs in the 5' UTR and initiates translation at an upstream start codon, compared to variant 1. The resulting protein (isoform 4) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.229920.1, SRR1660803.146470.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGCG AAACCCCAAA GAGTCAGGTA GGGACCCTGC TCTTCCGCAG CCGCAGCCGC 
CTGACGCACC TATCCCTGTC TCGACGGGCA TCCGAATCCT CCCTGTCGTC TGAATCCTCC 
GAATCTTCTG ATGCAGGTCT CTGCATGGAT TCCCCCAGCC CTATGGACCC CCACATGGCG 
GAGCAGACGT TTGAACAGGC CATCCAGGCA GCCAGCCGGA TCATTCGAAA CGAGCAGTTT 
GCCATCAGAC GCTTCCAGTC TATGCCGGTG AGGCTGCTGG GCCACAGCCC CGTGCTTCGG 
AACATCACCA ACTCCCAGGC GCCCGACGGC CGGAGGAAGA GCGAGGCGGG CAGTGGAGCT 
GCCAGCAGCT CTGGGGAAGA CAAGGAGAAT GATGGATTTG TCTTCAAGAT GCCATGGAAG 
CCCACACATC CCAGCTCCAC CCATGCTCTG GCAGAGTGGG CCAGCCGCAG GGAAGCCTTT 
GCCCAGAGAC CCAGCTCGGC CCCCGACCTG ATGTGTCTCA GTCCTGACCG GAAGATGGAA 
GTGGAGGAGC TCAGCCCCCT GGCCCTAGGT CGCTTCTCTC TGACCCCTGC AGAGGGGGAT 
ACTGAGGAAG ATGATGGATT TGTGGACATC CTAGAGAGTG ACTTAAAGGA TGATGATGCA 
GTTCCCCCAG GCATGGAGAG TCTCATTAGT GCCCCACTGG TCAAGACCTT GGAAAAGGAA 
GAGGAAAAGG ACCTCGTCAT GTACAGCAAG TGCCAGCGGC TCTTCCGCTC TCCGTCCATG 
CCCTGCAGCG TGATCCGGCC CATCCTCAAG AGGCTGGAGC GGCCCCAGGA CAGGGACACG 
CCCGTGCAGA ATAAGCGGAG GCGGAGCGTG ACCCCTCCTG AGGAGCAGCA GGAGGCTGAG 
GAACCTAAAG CCCGCGTCCT CCGCTCAAAA TCACTGTGTC ACGATGAGAT CGAGAACCTC 
CTGGACAGTG ACCACCGAGA GCTGATTGGA GATTACTCTA AGGCCTTCCT CCTACAGACA 
GTAGACGGAA AGCACCAAGA CCTCAAGTAC ATCTCACCAG AAACGATGGT GGCCCTATTG 
ACGGGCAAGT TCAGCAACAT CGTGGATAAG TTTGTGATTG TAGACTGCAG ATACCCCTAT 
GAATATGAAG GCGGGCACAT CAAGACTGCG GTGAACTTGC CCCTGGAACG CGACGCCGAG 
AGCTTCCTAC TGAAGAGCCC CATCGCGCCC TGTAGCCTGG ACAAGAGAGT CATCCTCATT 
TTCCACTGTG AATTCTCATC TGAGCGTGGG CCCCGCATGT GCCGTTTCAT CAGGGAACGA 
GACCGTGCTG TCAACGACTA CCCCAGCCTC TACTACCCTG AGATGTATAT CCTGAAAGGC 
GGCTACAAGG AGTTCTTCCC TCAGCACCCG AACTTCTGTG AACCCCAGGA CTACCGGCCC 
ATGAACCACG AGGCCTTCAA GGATGAGCTA AAGACCTTCC GCCTCAAGAC TCGCAGCTGG 
GCTGGGGAGC GGAGCCGGCG GGAGCTCTGT AGCCGGCTGC AGGACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274445.1
CDS	361..1911
Translation	MHSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287516.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287516.1

ORF Insert Sequence:

ATGCACAGCG AAACCCCAAA GAGTCAGGTA GGGACCCTGC TCTTCCGCAG CCGCAGCCGC 
CTGACGCACC TATCCCTGTC TCGACGGGCA TCCGAATCCT CCCTGTCGTC TGAATCCTCC 
GAATCTTCTG ATGCAGGTCT CTGCATGGAT TCCCCCAGCC CTATGGACCC CCACATGGCG 
GAGCAGACGT TTGAACAGGC CATCCAGGCA GCCAGCCGGA TCATTCGAAA CGAGCAGTTT 
GCCATCAGAC GCTTCCAGTC TATGCCGGTG AGGCTGCTGG GCCACAGCCC CGTGCTTCGG 
AACATCACCA ACTCCCAGGC GCCCGACGGC CGGAGGAAGA GCGAGGCGGG CAGTGGAGCT 
GCCAGCAGCT CTGGGGAAGA CAAGGAGAAT GATGGATTTG TCTTCAAGAT GCCATGGAAG 
CCCACACATC CCAGCTCCAC CCATGCTCTG GCAGAGTGGG CCAGCCGCAG GGAAGCCTTT 
GCCCAGAGAC CCAGCTCGGC CCCCGACCTG ATGTGTCTCA GTCCTGACCG GAAGATGGAA 
GTGGAGGAGC TCAGCCCCCT GGCCCTAGGT CGCTTCTCTC TGACCCCTGC AGAGGGGGAT 
ACTGAGGAAG ATGATGGATT TGTGGACATC CTAGAGAGTG ACTTAAAGGA TGATGATGCA 
GTTCCCCCAG GCATGGAGAG TCTCATTAGT GCCCCACTGG TCAAGACCTT GGAAAAGGAA 
GAGGAAAAGG ACCTCGTCAT GTACAGCAAG TGCCAGCGGC TCTTCCGCTC TCCGTCCATG 
CCCTGCAGCG TGATCCGGCC CATCCTCAAG AGGCTGGAGC GGCCCCAGGA CAGGGACACG 
CCCGTGCAGA ATAAGCGGAG GCGGAGCGTG ACCCCTCCTG AGGAGCAGCA GGAGGCTGAG 
GAACCTAAAG CCCGCGTCCT CCGCTCAAAA TCACTGTGTC ACGATGAGAT CGAGAACCTC 
CTGGACAGTG ACCACCGAGA GCTGATTGGA GATTACTCTA AGGCCTTCCT CCTACAGACA 
GTAGACGGAA AGCACCAAGA CCTCAAGTAC ATCTCACCAG AAACGATGGT GGCCCTATTG 
ACGGGCAAGT TCAGCAACAT CGTGGATAAG TTTGTGATTG TAGACTGCAG ATACCCCTAT 
GAATATGAAG GCGGGCACAT CAAGACTGCG GTGAACTTGC CCCTGGAACG CGACGCCGAG 
AGCTTCCTAC TGAAGAGCCC CATCGCGCCC TGTAGCCTGG ACAAGAGAGT CATCCTCATT 
TTCCACTGTG AATTCTCATC TGAGCGTGGG CCCCGCATGT GCCGTTTCAT CAGGGAACGA 
GACCGTGCTG TCAACGACTA CCCCAGCCTC TACTACCCTG AGATGTATAT CCTGAAAGGC 
GGCTACAAGG AGTTCTTCCC TCAGCACCCG AACTTCTGTG AACCCCAGGA CTACCGGCCC 
ATGAACCACG AGGCCTTCAA GGATGAGCTA AAGACCTTCC GCCTCAAGAC TCGCAGCTGG 
GCTGGGGAGC GGAGCCGGCG GGAGCTCTGT AGCCGGCTGC AGGACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_001287518.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001274447.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 6, mRNA.

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CloneID	OHu14337
Clone ID Related Accession (Same CDS sequence)	NM_001287518.1
Accession Version	NM_001287518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB477384.1, AK299028.1, AL109804.41 and DA107932.1. On Dec 17, 2013 this sequence version replaced XM_005260899.1. Transcript Variant: This variant (6) differs in the 5' UTR, initiates translation at an upstream start codon, and lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting protein (isoform 6) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.160537.1, SRR1803615.147781.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGCG AAACCCCAAA GAGTCAGGTA GGGACCCTGC TCTTCCGCAG CCGCAGCCGC 
CTGACGCACC TATCCCTGTC TCGACGGGCA TCCGAATCCT CCCTGTCGTC TGAATCCTCC 
GAATCTTCTG ATGCAGGTCT CTGCATGGAT TCCCCCAGCC CTATGGACCC CCACATGGCG 
GAGCAGACGT TTGAACAGGC CATCCAGGCA GCCAGCCGGA TCATTCGAAA CGAGCAGTTT 
GCCATCAGAC GCTTCCAGTC TATGCCGGTG AGGCTGCTGG GCCACAGCCC CGTGCTTCGG 
AACATCACCA ACTCCCAGGC GCCCGACGGC CGGAGGAAGA GCGAGGCGGG CAGTGGAGCT 
GCCAGCAGCT CTGGGGAAGA CAAGGAGAAT GATGGATTTG TCTTCAAGAT GCCATGGAAG 
CCCACACATC CCAGCTCCAC CCATGCTCTG GCAGAGTGGG CCAGCCGCAG GGAAGCCTTT 
GCCCAGAGAC CCAGCTCGGC CCCCGACCTG ATGTGTCTCA GTCCTGACCG GAAGATGGAA 
GTGGAGGAGC TCAGCCCCCT GGCCCTAGGT CGCTTCTCTC TGACCCCTGC AGAGGGGGAT 
ACTGAGGAAG ATGATGGATT TGTGGACATC CTAGAGAGTG ACTTAAAGGA CCTCGTCATG 
TACAGCAAGT GCCAGCGGCT CTTCCGCTCT CCGTCCATGC CCTGCAGCGT GATCCGGCCC 
ATCCTCAAGA GGCTGGAGCG GCCCCAGGAC AGGGACACGC CCGTGCAGAA TAAGCGGAGG 
CGGAGCGTGA CCCCTCCTGA GGAGCAGCAG GAGGCTGAGG AACCTAAAGC CCGCGTCCTC 
CGCTCAAAAT CACTGTGTCA CGATGAGATC GAGAACCTCC TGGACAGTGA CCACCGAGAG 
CTGATTGGAG ATTACTCTAA GGCCTTCCTC CTACAGACAG TAGACGGAAA GCACCAAGAC 
CTCAAGTACA TCTCACCAGA AACGATGGTG GCCCTATTGA CGGGCAAGTT CAGCAACATC 
GTGGATAAGT TTGTGATTGT AGACTGCAGA TACCCCTATG AATATGAAGG CGGGCACATC 
AAGACTGCGG TGAACTTGCC CCTGGAACGC GACGCCGAGA GCTTCCTACT GAAGAGCCCC 
ATCGCGCCCT GTAGCCTGGA CAAGAGAGTC ATCCTCATTT TCCACTGTGA ATTCTCATCT 
GAGCGTGGGC CCCGCATGTG CCGTTTCATC AGGGAACGAG ACCGTGCTGT CAACGACTAC 
CCCAGCCTCT ACTACCCTGA GATGTATATC CTGAAAGGCG GCTACAAGGA GTTCTTCCCT 
CAGCACCCGA ACTTCTGTGA ACCCCAGGAC TACCGGCCCA TGAACCACGA GGCCTTCAAG 
GATGAGCTAA AGACCTTCCG CCTCAAGACT CGCAGCTGGG CTGGGGAGCG GAGCCGGCGG 
GAGCTCTGTA GCCGGCTGCA GGACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274447.1
CDS	361..1830
Translation	MHSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_001287518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287518.1

ORF Insert Sequence:

ATGCACAGCG AAACCCCAAA GAGTCAGGTA GGGACCCTGC TCTTCCGCAG CCGCAGCCGC 
CTGACGCACC TATCCCTGTC TCGACGGGCA TCCGAATCCT CCCTGTCGTC TGAATCCTCC 
GAATCTTCTG ATGCAGGTCT CTGCATGGAT TCCCCCAGCC CTATGGACCC CCACATGGCG 
GAGCAGACGT TTGAACAGGC CATCCAGGCA GCCAGCCGGA TCATTCGAAA CGAGCAGTTT 
GCCATCAGAC GCTTCCAGTC TATGCCGGTG AGGCTGCTGG GCCACAGCCC CGTGCTTCGG 
AACATCACCA ACTCCCAGGC GCCCGACGGC CGGAGGAAGA GCGAGGCGGG CAGTGGAGCT 
GCCAGCAGCT CTGGGGAAGA CAAGGAGAAT GATGGATTTG TCTTCAAGAT GCCATGGAAG 
CCCACACATC CCAGCTCCAC CCATGCTCTG GCAGAGTGGG CCAGCCGCAG GGAAGCCTTT 
GCCCAGAGAC CCAGCTCGGC CCCCGACCTG ATGTGTCTCA GTCCTGACCG GAAGATGGAA 
GTGGAGGAGC TCAGCCCCCT GGCCCTAGGT CGCTTCTCTC TGACCCCTGC AGAGGGGGAT 
ACTGAGGAAG ATGATGGATT TGTGGACATC CTAGAGAGTG ACTTAAAGGA CCTCGTCATG 
TACAGCAAGT GCCAGCGGCT CTTCCGCTCT CCGTCCATGC CCTGCAGCGT GATCCGGCCC 
ATCCTCAAGA GGCTGGAGCG GCCCCAGGAC AGGGACACGC CCGTGCAGAA TAAGCGGAGG 
CGGAGCGTGA CCCCTCCTGA GGAGCAGCAG GAGGCTGAGG AACCTAAAGC CCGCGTCCTC 
CGCTCAAAAT CACTGTGTCA CGATGAGATC GAGAACCTCC TGGACAGTGA CCACCGAGAG 
CTGATTGGAG ATTACTCTAA GGCCTTCCTC CTACAGACAG TAGACGGAAA GCACCAAGAC 
CTCAAGTACA TCTCACCAGA AACGATGGTG GCCCTATTGA CGGGCAAGTT CAGCAACATC 
GTGGATAAGT TTGTGATTGT AGACTGCAGA TACCCCTATG AATATGAAGG CGGGCACATC 
AAGACTGCGG TGAACTTGCC CCTGGAACGC GACGCCGAGA GCTTCCTACT GAAGAGCCCC 
ATCGCGCCCT GTAGCCTGGA CAAGAGAGTC ATCCTCATTT TCCACTGTGA ATTCTCATCT 
GAGCGTGGGC CCCGCATGTG CCGTTTCATC AGGGAACGAG ACCGTGCTGT CAACGACTAC 
CCCAGCCTCT ACTACCCTGA GATGTATATC CTGAAAGGCG GCTACAAGGA GTTCTTCCCT 
CAGCACCCGA ACTTCTGTGA ACCCCAGGAC TACCGGCCCA TGAACCACGA GGCCTTCAAG 
GATGAGCTAA AGACCTTCCG CCTCAAGACT CGCAGCTGGG CTGGGGAGCG GAGCCGGCGG 
GAGCTCTGTA GCCGGCTGCA GGACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_004358.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004349.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 2, mRNA.

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CloneID	OHu14355
Clone ID Related Accession (Same CDS sequence)	NM_004358.4
Accession Version	NM_004358.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1701bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092713.1, BC051711.1, S78187.1, BX640836.1 and DA107932.1. On Dec 17, 2013 this sequence version replaced NM_004358.3. Transcript Variant: This variant (2), also known as CDC25B1, uses an alternate splice site in the 5' coding region, compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S78187.1, M81934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGC CCCAGCCGGA GCCCGCGCCA GGCTCGGCTC TCAGTCCAGC AGGCGTGTGC 
GGTGGCGCCC AGCGTCCGGG CCACCTCCCG GGCCTCCTGC TGGGATCTCA TGGCCTCCTG 
GGGTCCCCGG TGCGGGCGGC CGCTTCCTCG CCGGTCACCA CCCTCACCCA GACCATGCAC 
GACCTCGCCG GGCTCGGCAG CCGCAGCCGC CTGACGCACC TATCCCTGTC TCGACGGGCA 
TCCGAATCCT CCCTGTCGTC TGAATCCTCC GAATCTTCTG ATGCAGGTCT CTGCATGGAT 
TCCCCCAGCC CTATGGACCC CCACATGGCG GAGCAGACGT TTGAACAGGC CATCCAGGCA 
GCCAGCCGGA TCATTCGAAA CGAGCAGTTT GCCATCAGAC GCTTCCAGTC TATGCCGGTG 
AGGCTGCTGG GCCACAGCCC CGTGCTTCGG AACATCACCA ACTCCCAGGC GCCCGACGGC 
CGGAGGAAGA GCGAGGCGGG CAGTGGAGCT GCCAGCAGCT CTGGGGAAGA CAAGGAGAAT 
GATGGATTTG TCTTCAAGAT GCCATGGAAG CCCACACATC CCAGCTCCAC CCATGCTCTG 
GCAGAGTGGG CCAGCCGCAG GGAAGCCTTT GCCCAGAGAC CCAGCTCGGC CCCCGACCTG 
ATGTGTCTCA GTCCTGACCG GAAGATGGAA GTGGAGGAGC TCAGCCCCCT GGCCCTAGGT 
CGCTTCTCTC TGACCCCTGC AGAGGGGGAT ACTGAGGAAG ATGATGGATT TGTGGACATC 
CTAGAGAGTG ACTTAAAGGA TGATGATGCA GTTCCCCCAG GCATGGAGAG TCTCATTAGT 
GCCCCACTGG TCAAGACCTT GGAAAAGGAA GAGGAAAAGG ACCTCGTCAT GTACAGCAAG 
TGCCAGCGGC TCTTCCGCTC TCCGTCCATG CCCTGCAGCG TGATCCGGCC CATCCTCAAG 
AGGCTGGAGC GGCCCCAGGA CAGGGACACG CCCGTGCAGA ATAAGCGGAG GCGGAGCGTG 
ACCCCTCCTG AGGAGCAGCA GGAGGCTGAG GAACCTAAAG CCCGCGTCCT CCGCTCAAAA 
TCACTGTGTC ACGATGAGAT CGAGAACCTC CTGGACAGTG ACCACCGAGA GCTGATTGGA 
GATTACTCTA AGGCCTTCCT CCTACAGACA GTAGACGGAA AGCACCAAGA CCTCAAGTAC 
ATCTCACCAG AAACGATGGT GGCCCTATTG ACGGGCAAGT TCAGCAACAT CGTGGATAAG 
TTTGTGATTG TAGACTGCAG ATACCCCTAT GAATATGAAG GCGGGCACAT CAAGACTGCG 
GTGAACTTGC CCCTGGAACG CGACGCCGAG AGCTTCCTAC TGAAGAGCCC CATCGCGCCC 
TGTAGCCTGG ACAAGAGAGT CATCCTCATT TTCCACTGTG AATTCTCATC TGAGCGTGGG 
CCCCGCATGT GCCGTTTCAT CAGGGAACGA GACCGTGCTG TCAACGACTA CCCCAGCCTC 
TACTACCCTG AGATGTATAT CCTGAAAGGC GGCTACAAGG AGTTCTTCCC TCAGCACCCG 
AACTTCTGTG AACCCCAGGA CTACCGGCCC ATGAACCACG AGGCCTTCAA GGATGAGCTA 
AAGACCTTCC GCCTCAAGAC TCGCAGCTGG GCTGGGGAGC GGAGCCGGCG GGAGCTCTGT 
AGCCGGCTGC AGGACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004349.1
CDS	794..2494
Translation	MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_004358.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004358.4

ORF Insert Sequence:

ATGGAGGTGC CCCAGCCGGA GCCCGCGCCA GGCTCGGCTC TCAGTCCAGC AGGCGTGTGC 
GGTGGCGCCC AGCGTCCGGG CCACCTCCCG GGCCTCCTGC TGGGATCTCA TGGCCTCCTG 
GGGTCCCCGG TGCGGGCGGC CGCTTCCTCG CCGGTCACCA CCCTCACCCA GACCATGCAC 
GACCTCGCCG GGCTCGGCAG CCGCAGCCGC CTGACGCACC TATCCCTGTC TCGACGGGCA 
TCCGAATCCT CCCTGTCGTC TGAATCCTCC GAATCTTCTG ATGCAGGTCT CTGCATGGAT 
TCCCCCAGCC CTATGGACCC CCACATGGCG GAGCAGACGT TTGAACAGGC CATCCAGGCA 
GCCAGCCGGA TCATTCGAAA CGAGCAGTTT GCCATCAGAC GCTTCCAGTC TATGCCGGTG 
AGGCTGCTGG GCCACAGCCC CGTGCTTCGG AACATCACCA ACTCCCAGGC GCCCGACGGC 
CGGAGGAAGA GCGAGGCGGG CAGTGGAGCT GCCAGCAGCT CTGGGGAAGA CAAGGAGAAT 
GATGGATTTG TCTTCAAGAT GCCATGGAAG CCCACACATC CCAGCTCCAC CCATGCTCTG 
GCAGAGTGGG CCAGCCGCAG GGAAGCCTTT GCCCAGAGAC CCAGCTCGGC CCCCGACCTG 
ATGTGTCTCA GTCCTGACCG GAAGATGGAA GTGGAGGAGC TCAGCCCCCT GGCCCTAGGT 
CGCTTCTCTC TGACCCCTGC AGAGGGGGAT ACTGAGGAAG ATGATGGATT TGTGGACATC 
CTAGAGAGTG ACTTAAAGGA TGATGATGCA GTTCCCCCAG GCATGGAGAG TCTCATTAGT 
GCCCCACTGG TCAAGACCTT GGAAAAGGAA GAGGAAAAGG ACCTCGTCAT GTACAGCAAG 
TGCCAGCGGC TCTTCCGCTC TCCGTCCATG CCCTGCAGCG TGATCCGGCC CATCCTCAAG 
AGGCTGGAGC GGCCCCAGGA CAGGGACACG CCCGTGCAGA ATAAGCGGAG GCGGAGCGTG 
ACCCCTCCTG AGGAGCAGCA GGAGGCTGAG GAACCTAAAG CCCGCGTCCT CCGCTCAAAA 
TCACTGTGTC ACGATGAGAT CGAGAACCTC CTGGACAGTG ACCACCGAGA GCTGATTGGA 
GATTACTCTA AGGCCTTCCT CCTACAGACA GTAGACGGAA AGCACCAAGA CCTCAAGTAC 
ATCTCACCAG AAACGATGGT GGCCCTATTG ACGGGCAAGT TCAGCAACAT CGTGGATAAG 
TTTGTGATTG TAGACTGCAG ATACCCCTAT GAATATGAAG GCGGGCACAT CAAGACTGCG 
GTGAACTTGC CCCTGGAACG CGACGCCGAG AGCTTCCTAC TGAAGAGCCC CATCGCGCCC 
TGTAGCCTGG ACAAGAGAGT CATCCTCATT TTCCACTGTG AATTCTCATC TGAGCGTGGG 
CCCCGCATGT GCCGTTTCAT CAGGGAACGA GACCGTGCTG TCAACGACTA CCCCAGCCTC 
TACTACCCTG AGATGTATAT CCTGAAAGGC GGCTACAAGG AGTTCTTCCC TCAGCACCCG 
AACTTCTGTG AACCCCAGGA CTACCGGCCC ATGAACCACG AGGCCTTCAA GGATGAGCTA 
AAGACCTTCC GCCTCAAGAC TCGCAGCTGG GCTGGGGAGC GGAGCCGGCG GGAGCTCTGT 
AGCCGGCTGC AGGACCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC25B ( NM_021872.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_068658.1 Homo sapiens cell division cycle 25B (CDC25B), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14356
Clone ID Related Accession (Same CDS sequence)	NM_021872.3
Accession Version	NM_021872.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1620bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	M-phase inducer phosphatase 2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK092713.1, BC051711.1, S78187.1, Z68092.1, BX640836.1 and DA107932.1. On Dec 17, 2013 this sequence version replaced NM_021872.2. Transcript Variant: This variant (3), also known as CDC25B2, lacks an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3) compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.13775.1, SRR1803613.272094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGTGC CCCAGCCGGA GCCCGCGCCA GGCTCGGCTC TCAGTCCAGC AGGCGTGTGC 
GGTGGCGCCC AGCGTCCGGG CCACCTCCCG GGCCTCCTGC TGGGATCTCA TGGCCTCCTG 
GGGTCCCCGG TGCGGGCGGC CGCTTCCTCG CCGGTCACCA CCCTCACCCA GACCATGCAC 
GACCTCGCCG GGCTCGGCAG CGAAACCCCA AAGAGTCAGG TAGGGACCCT GCTCTTCCGC 
AGCCGCAGCC GCCTGACGCA CCTATCCCTG TCTCGACGGG CATCCGAATC CTCCCTGTCG 
TCTGAATCCT CCGAATCTTC TGATGCAGGT CTCTGCATGG ATTCCCCCAG CCCTATGGAC 
CCCCACATGG CGGAGCAGAC GTTTGAACAG GCCATCCAGG CAGCCAGCCG GATCATTCGA 
AACGAGCAGT TTGCCATCAG ACGCTTCCAG TCTATGCCGG ATGGATTTGT CTTCAAGATG 
CCATGGAAGC CCACACATCC CAGCTCCACC CATGCTCTGG CAGAGTGGGC CAGCCGCAGG 
GAAGCCTTTG CCCAGAGACC CAGCTCGGCC CCCGACCTGA TGTGTCTCAG TCCTGACCGG 
AAGATGGAAG TGGAGGAGCT CAGCCCCCTG GCCCTAGGTC GCTTCTCTCT GACCCCTGCA 
GAGGGGGATA CTGAGGAAGA TGATGGATTT GTGGACATCC TAGAGAGTGA CTTAAAGGAT 
GATGATGCAG TTCCCCCAGG CATGGAGAGT CTCATTAGTG CCCCACTGGT CAAGACCTTG 
GAAAAGGAAG AGGAAAAGGA CCTCGTCATG TACAGCAAGT GCCAGCGGCT CTTCCGCTCT 
CCGTCCATGC CCTGCAGCGT GATCCGGCCC ATCCTCAAGA GGCTGGAGCG GCCCCAGGAC 
AGGGACACGC CCGTGCAGAA TAAGCGGAGG CGGAGCGTGA CCCCTCCTGA GGAGCAGCAG 
GAGGCTGAGG AACCTAAAGC CCGCGTCCTC CGCTCAAAAT CACTGTGTCA CGATGAGATC 
GAGAACCTCC TGGACAGTGA CCACCGAGAG CTGATTGGAG ATTACTCTAA GGCCTTCCTC 
CTACAGACAG TAGACGGAAA GCACCAAGAC CTCAAGTACA TCTCACCAGA AACGATGGTG 
GCCCTATTGA CGGGCAAGTT CAGCAACATC GTGGATAAGT TTGTGATTGT AGACTGCAGA 
TACCCCTATG AATATGAAGG CGGGCACATC AAGACTGCGG TGAACTTGCC CCTGGAACGC 
GACGCCGAGA GCTTCCTACT GAAGAGCCCC ATCGCGCCCT GTAGCCTGGA CAAGAGAGTC 
ATCCTCATTT TCCACTGTGA ATTCTCATCT GAGCGTGGGC CCCGCATGTG CCGTTTCATC 
AGGGAACGAG ACCGTGCTGT CAACGACTAC CCCAGCCTCT ACTACCCTGA GATGTATATC 
CTGAAAGGCG GCTACAAGGA GTTCTTCCCT CAGCACCCGA ACTTCTGTGA ACCCCAGGAC 
TACCGGCCCA TGAACCACGA GGCCTTCAAG GATGAGCTAA AGACCTTCCG CCTCAAGACT 
CGCAGCTGGG CTGGGGAGCG GAGCCGGCGG GAGCTCTGTA GCCGGCTGCA GGACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068658.1
CDS	794..2413
Translation	MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ

Target ORF information:

RefSeq Version	NM_021872.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cell division cycle 25B (CDC25B), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021872.3

ORF Insert Sequence:

ATGGAGGTGC CCCAGCCGGA GCCCGCGCCA GGCTCGGCTC TCAGTCCAGC AGGCGTGTGC 
GGTGGCGCCC AGCGTCCGGG CCACCTCCCG GGCCTCCTGC TGGGATCTCA TGGCCTCCTG 
GGGTCCCCGG TGCGGGCGGC CGCTTCCTCG CCGGTCACCA CCCTCACCCA GACCATGCAC 
GACCTCGCCG GGCTCGGCAG CGAAACCCCA AAGAGTCAGG TAGGGACCCT GCTCTTCCGC 
AGCCGCAGCC GCCTGACGCA CCTATCCCTG TCTCGACGGG CATCCGAATC CTCCCTGTCG 
TCTGAATCCT CCGAATCTTC TGATGCAGGT CTCTGCATGG ATTCCCCCAG CCCTATGGAC 
CCCCACATGG CGGAGCAGAC GTTTGAACAG GCCATCCAGG CAGCCAGCCG GATCATTCGA 
AACGAGCAGT TTGCCATCAG ACGCTTCCAG TCTATGCCGG ATGGATTTGT CTTCAAGATG 
CCATGGAAGC CCACACATCC CAGCTCCACC CATGCTCTGG CAGAGTGGGC CAGCCGCAGG 
GAAGCCTTTG CCCAGAGACC CAGCTCGGCC CCCGACCTGA TGTGTCTCAG TCCTGACCGG 
AAGATGGAAG TGGAGGAGCT CAGCCCCCTG GCCCTAGGTC GCTTCTCTCT GACCCCTGCA 
GAGGGGGATA CTGAGGAAGA TGATGGATTT GTGGACATCC TAGAGAGTGA CTTAAAGGAT 
GATGATGCAG TTCCCCCAGG CATGGAGAGT CTCATTAGTG CCCCACTGGT CAAGACCTTG 
GAAAAGGAAG AGGAAAAGGA CCTCGTCATG TACAGCAAGT GCCAGCGGCT CTTCCGCTCT 
CCGTCCATGC CCTGCAGCGT GATCCGGCCC ATCCTCAAGA GGCTGGAGCG GCCCCAGGAC 
AGGGACACGC CCGTGCAGAA TAAGCGGAGG CGGAGCGTGA CCCCTCCTGA GGAGCAGCAG 
GAGGCTGAGG AACCTAAAGC CCGCGTCCTC CGCTCAAAAT CACTGTGTCA CGATGAGATC 
GAGAACCTCC TGGACAGTGA CCACCGAGAG CTGATTGGAG ATTACTCTAA GGCCTTCCTC 
CTACAGACAG TAGACGGAAA GCACCAAGAC CTCAAGTACA TCTCACCAGA AACGATGGTG 
GCCCTATTGA CGGGCAAGTT CAGCAACATC GTGGATAAGT TTGTGATTGT AGACTGCAGA 
TACCCCTATG AATATGAAGG CGGGCACATC AAGACTGCGG TGAACTTGCC CCTGGAACGC 
GACGCCGAGA GCTTCCTACT GAAGAGCCCC ATCGCGCCCT GTAGCCTGGA CAAGAGAGTC 
ATCCTCATTT TCCACTGTGA ATTCTCATCT GAGCGTGGGC CCCGCATGTG CCGTTTCATC 
AGGGAACGAG ACCGTGCTGT CAACGACTAC CCCAGCCTCT ACTACCCTGA GATGTATATC 
CTGAAAGGCG GCTACAAGGA GTTCTTCCCT CAGCACCCGA ACTTCTGTGA ACCCCAGGAC 
TACCGGCCCA TGAACCACGA GGCCTTCAAG GATGAGCTAA AGACCTTCCG CCTCAAGACT 
CGCAGCTGGG CTGGGGAGCG GAGCCGGCGG GAGCTCTGTA GCCGGCTGCA GGACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	CDC25B
Entrez Gene ID	994
Full Name	cell division cycle 25B
General protein information	Preferred Names cell division cycle 25B Names M-phase inducer phosphatase 2 dual specificity phosphatase Cdc25B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p13
Summary	CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].
Disorder MIM:	116949

Homo sapiens (human)	CDC25B	NP_068659.1
Bos taurus (cattle)	CDC25B	NP_001178344.1
Pan troglodytes (chimpanzee)	CDC25B	XP_001162751.1
Mus musculus (house mouse)	Cdc25b	NP_075606.1
Rattus norvegicus (Norway rat)	Cdc25b	NP_598256.1
Xenopus tropicalis (tropical clawed frog)	cdc25b	XP_002936339.1
Canis lupus familiaris (dog)	CDC25B	XP_850186.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

CDC25B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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