DCLRE1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCLRE1A
Entrez Gene ID	9937
Full Name	DNA cross-link repair 1A
Synonyms	PSO2,SNM1,SNM1A
General protein information	Preferred Names DNA cross-link repair 1A Names DNA cross-link repair 1A protein DNA-crosslink repair gene SNM1 SNM1 homolog A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q25.3
Summary	This gene encodes a conserved protein that is involved in the repair of DNA interstrand cross-links. DNA cross-links suppress transcription, replication, and DNA segregation. The encoded protein is a regulator of the mitotic cell cycle checkpoint. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012].
Disorder MIM:	609682

mRNA and Protein(s)

mRNA	Protein	Name
NM_001271816.2	NP_001258745.1	DNA cross-link repair 1A protein
NM_014881.4	NP_055696.3	DNA cross-link repair 1A protein
NM_001271816.1	NP_001258745.1	DNA cross-link repair 1A protein
NM_014881.5	NP_055696.3	DNA cross-link repair 1A protein
XM_006718090.1	XP_006718153.1	DNA cross-link repair 1A protein isoform X1
XM_011540429.1	XP_011538731.1	DNA cross-link repair 1A protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DCLRE1A	XP_508045.2
Oryza sativa (rice)	Os04g0401800	NP_001052690.1
Bos taurus (cattle)	DCLRE1A	XP_002698556.1
Arabidopsis thaliana (thale cress)	SNM1	NP_001030773.1
Canis lupus familiaris (dog)	DCLRE1A	XP_005637802.1
Gallus gallus (chicken)	DCLRE1A	NP_001008683.1
Homo sapiens (human)	DCLRE1A	NP_001258745.1
Rattus norvegicus (Norway rat)	Dclre1a	NP_001099671.1
	DCLRE1A	XP_001090942.1
Mus musculus (house mouse)	Dclre1a	NP_061301.3
Danio rerio (zebrafish)	dclre1a	NP_001018385.3
Xenopus tropicalis (tropical clawed frog)	dclre1a	XP_004919436.1

Gene Ontology
Bibliography

Related articles in PubMed

RPA activates the XPF-ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks.
Abdullah UB, McGouran JF, Brolih S, Ptchelkine D, El-Sagheer AH, Brown T, McHugh PJ
The EMBO journal36(14)2047-2060(2017 07)

The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Allerston CK, Lee SY, Newman JA, Schofield CJ, McHugh PJ, Gileadi O
Nucleic acids research43(22)11047-60(2015 Dec)

Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.
Sengerov? B, Allerston CK, Abu M, Lee SY, Hartley J, Kiakos K, Schofield CJ, Hartley JA, Gileadi O, McHugh PJ
The Journal of biological chemistry287(31)26254-67(2012 Jul)

Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.
Wang AT, Sengerov? B, Cattell E, Inagawa T, Hartley JM, Kiakos K, Burgess-Brown NA, Swift LP, Enzlin JH, Schofield CJ, Gileadi O, Hartley JA, McHugh PJ
Genes & development25(17)1859-70(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Strikingly, the addition of the single-stranded DNA (ssDNA)-binding replication protein A (RPA) selectively restores XPF-ERCC1 endonuclease activity on this structure. The 5'-3' exonuclease SNM1A can load from the XPF-ERCC1-RPA-induced incisions and digest past the crosslink to quantitatively complete the unhooking reaction.
Title: RPA activates the XPF-ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks.

Different charge distributions along the DNA binding groove may account for the drastic difference in processivity and DNA digestion efficiency, including that of damaged substrates, between SNM1A and SNM1B.
Title: The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.

differences in the substrate selectivities of SNM1A and SNM1B are likely to be relevant to their in vivo roles
Title: Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.

collaboration between hSNM1A and XPF-ERCC1 is necessary to initiate interstrand cross-link repair in replicating human cells
Title: Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.

RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger
Title: RAD18-dependent recruitment of SNM1A to DNA repair complexes by a ubiquitin-binding zinc finger.

The following DCLRE1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCLRE1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03901	NM_014881.5 Latest version!	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu03901	NM_001271816.2 Latest version!	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu03901	XM_011540429.1 Latest version!	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu03901	XM_006718090.1 Latest version!	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
View Old Accession Versions >>
OHu03901	NM_001271816.1	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
OHu03901	NM_014881.4	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCLRE1A ( NM_001271816.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258745.1 Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03901
Clone ID Related Accession (Same CDS sequence)	NM_001271816.2 , NM_014881.4 , NM_001271816.1 , NM_014881.5 , XM_006718090.1 , XM_011540429.1
Accession Version	NM_001271816.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	DNA cross-link repair 1A protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB224073.1 and D42045.1. On May 31, 2019 this sequence version replaced NM_001271816.1. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.26689.1, SRR1660803.247075.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258745.1
CDS	707..3829
Translation	MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNAGCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWHVFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLEERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANNSEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGPLLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAASVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLSSENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIGVYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSERSQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKTCPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY

Target ORF information:

RefSeq Version	NM_001271816.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271816.2

ORF Insert Sequence:

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCLRE1A ( NM_014881.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055696.3 Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03901
Clone ID Related Accession (Same CDS sequence)	NM_001271816.2 , NM_014881.4 , NM_001271816.1 , NM_014881.5 , XM_006718090.1 , XM_011540429.1
Accession Version	NM_014881.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	DNA cross-link repair 1A protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA456679.1, D42045.1 and H98725.1. On Nov 28, 2012 this sequence version replaced NM_014881.3. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.122951.1, SRR1803613.45413.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055696.3
CDS	1035..4157
Translation	MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNAGCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWHVFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLEERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANNSEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGPLLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAASVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLSSENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIGVYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSERSQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKTCPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY

Target ORF information:

RefSeq Version	NM_014881.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014881.4

ORF Insert Sequence:

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCLRE1A ( NM_001271816.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258745.1 Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03901
Clone ID Related Accession (Same CDS sequence)	NM_001271816.2 , NM_014881.4 , NM_001271816.1 , NM_014881.5 , XM_006718090.1 , XM_011540429.1
Accession Version	NM_001271816.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	DNA cross-link repair 1A protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB224073.1, D42045.1 and H98725.1. Transcript Variant: This variant (1) represents the shorter transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.21631.1, SRR1803611.91673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258745.1
CDS	717..3839
Translation	MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNAGCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWHVFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLEERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANNSEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGPLLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAASVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLSSENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIGVYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSERSQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKTCPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY

Target ORF information:

RefSeq Version	NM_001271816.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271816.1

ORF Insert Sequence:

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCLRE1A ( NM_014881.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055696.3 Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03901
Clone ID Related Accession (Same CDS sequence)	NM_001271816.2 , NM_014881.4 , NM_001271816.1 , NM_014881.5 , XM_006718090.1 , XM_011540429.1
Accession Version	NM_014881.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	DNA cross-link repair 1A protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from D42045.1. On Nov 22, 2018 this sequence version replaced NM_014881.4. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D42045.1, SRR1803615.122951.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361384.7/ ENSP00000355185.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055696.3
CDS	901..4023
Translation	MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNAGCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWHVFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLEERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANNSEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGPLLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAASVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLSSENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIGVYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSERSQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKTCPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY

Target ORF information:

RefSeq Version	NM_014881.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014881.5

ORF Insert Sequence:

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCLRE1A ( XM_006718090.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718153.1 Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant X1, mRNA.

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CloneID	OHu03901
Clone ID Related Accession (Same CDS sequence)	NM_001271816.2 , NM_014881.4 , NM_001271816.1 , NM_014881.5 , XM_006718090.1 , XM_011540429.1
Accession Version	XM_006718090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA cross-link repair 1A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718153.1
CDS	729..3851
Translation	MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNAGCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWHVFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLEERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANNSEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGPLLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAASVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLSSENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIGVYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSERSQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKTCPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY

Target ORF information:

RefSeq Version	XM_006718090.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718090.1

ORF Insert Sequence:

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCLRE1A ( XM_011540429.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538731.1 Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant X2, mRNA.

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CloneID	OHu03901
Clone ID Related Accession (Same CDS sequence)	NM_001271816.2 , NM_014881.4 , NM_001271816.1 , NM_014881.5 , XM_006718090.1 , XM_011540429.1
Accession Version	XM_011540429.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA cross-link repair 1A protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538731.1
CDS	785..3907
Translation	MLEDISEEDIWEYKSKRKPKRVDPNNGSKNILKSVEKATDGKYQSKRSRNRKRAAEAKEVKDHEVPLGNAGCQTSVASSQNSSCGDGIQQTQDKETTPGKLCRTQKSQHVSPKIRPVYDGYCPNCQMPFSSLIGQTPRWHVFECLDSPPRSETECPDGLLCTSTIPFHYKRYTHFLLAQSRAGDHPFSSPSPASGGSFSETKSGVLCSLEERWSSYQNQTDNSVSNDPLLMTQYFKKSPSLTEASEKISTHIQTSQQALQFTDFVENDKLVGVALRLANNSEHINLPLPENDFSDCEISYSPLQSDEDTHDIDEKPDDSQEQLFFTESSKDGSLEEDDDSCGFFKKRHGPLLKDQDESCPKVNSFLTRDKYDEGLYRFNSLNDLSQPISQNNESTLPYDLACTGGDFVLFPPALAGKLAASVHQATKAKPDEPEFHSAQSNKQKQVIEESSVYNQVSLPLVKSLMLKPFESQVEGYLSSQPTQNTIRKLSSENLNAKNNTNSACFCRKALEGVPVGKATILNTENLSSTPAPKYLKILPSGLKYNARHPSTKVMKQMDIGVYFGLPPKRKEEKLLGESALEGINLNPVPSPNQKRSSQCKRKAEKSLSDLEFDASTLHESQLSVELSSERSQRQKKRCRKSNSLQEGACQKRSDHLINTESEAVNLSKVKVFTKSAHGGLQRGNKKIPESSNVGGSRKKTCPFYKKIPGTGFTVDAFQYGVVEGCTAYFLTHFHSDHYAGLSKHFTFPVYCSEITGNLLKNKLHVQEQYIHPLPLDTECIVNGVKVVLLDANHCPGAVMILFYLPNGTVILHTGDFRADPSMERSLLADQKVHMLYLDTTYCSPEYTFPSQQEVIRFAINTAFEAVTLNPHALVVCGTYSIGKEKVFLAIADVLGSKVGMSQEKYKTLQCLNIPEINSLITTDMCSSLVHLLPMMQINFKGLQSHLKKCGGKYNQILAFRPTGWTHSNKFTRIADVIPQTKGNISIYGIPYSEHSSYLEMKRFVQWLKPQKIIPTVNVGTWKSRSTMEKYFREWKLEAGY

Target ORF information:

RefSeq Version	XM_011540429.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA cross-link repair 1A (DCLRE1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540429.1

ORF Insert Sequence:

ATGTTAGAAG ACATTTCCGA AGAAGACATT TGGGAATACA AATCTAAAAG AAAACCAAAA 
CGAGTTGATC CAAATAATGG CTCTAAAAAT ATTCTAAAAT CTGTTGAAAA AGCAACAGAT 
GGAAAATACC AGTCAAAACG GAGTAGAAAC AGAAAAAGAG CCGCAGAAGC TAAAGAGGTG 
AAGGACCATG AAGTGCCCCT TGGAAATGCA GGTTGTCAGA CTTCTGTTGC TTCTAGTCAG 
AATTCAAGTT GTGGAGATGG TATTCAGCAG ACCCAAGACA AGGAAACTAC TCCAGGAAAA 
CTCTGTAGAA CTCAAAAAAG CCAACACGTG TCCCCAAAGA TACGTCCAGT TTATGATGGA 
TACTGTCCAA ATTGCCAGAT GCCTTTTTCC TCATTGATAG GGCAGACACC TCGATGGCAT 
GTTTTTGAAT GTTTGGATTC TCCACCACGC TCTGAAACAG AGTGTCCTGA TGGTCTTCTG 
TGTACCTCAA CCATTCCTTT TCATTACAAG AGATACACTC ACTTCCTGCT AGCTCAAAGC 
AGGGCTGGTG ATCATCCTTT TAGCAGCCCA TCACCTGCGT CAGGTGGCAG TTTCAGTGAG 
ACTAAGTCAG GCGTCCTTTG TAGCCTTGAG GAAAGATGGT CTTCGTATCA GAACCAAACT 
GATAACTCGG TTTCAAATGA TCCCTTATTG ATGACACAGT ATTTTAAAAA GTCTCCGTCT 
CTGACTGAAG CCAGTGAAAA GATTTCTACT CATATCCAAA CATCCCAACA AGCTCTACAA 
TTTACAGATT TTGTTGAGAA TGACAAACTA GTGGGAGTTG CTTTGCGTCT TGCAAACAAC 
TCAGAACACA TAAATTTGCC ATTGCCAGAA AATGACTTCA GTGACTGTGA AATCTCCTAT 
TCTCCACTTC AAAGTGATGA AGACACTCAT GATATCGATG AAAAACCGGA TGATTCACAA 
GAACAACTGT TTTTTACCGA AAGCTCAAAA GATGGCAGCC TCGAAGAAGA TGATGACAGC 
TGTGGTTTTT TTAAAAAACG ACATGGTCCC TTACTGAAGG ACCAGGATGA GAGCTGCCCC 
AAAGTGAACA GCTTCTTAAC TCGGGATAAG TATGATGAAG GATTGTATAG ATTCAATAGT 
CTAAATGATT TGTCTCAACC TATTTCTCAA AATAATGAGA GTACTTTGCC TTATGATCTG 
GCATGTACTG GTGGTGATTT TGTGTTGTTT CCACCTGCAT TGGCAGGGAA GCTTGCTGCT 
TCTGTTCATC AGGCAACTAA AGCAAAACCT GATGAGCCAG AATTTCACTC AGCTCAATCA 
AATAAACAGA AACAGGTAAT TGAAGAATCA TCTGTTTACA ATCAAGTTTC TCTTCCGTTA 
GTTAAGAGTT TAATGTTGAA ACCTTTTGAA AGTCAGGTAG AAGGGTATCT TTCTTCCCAA 
CCAACCCAAA ATACAATTAG AAAATTATCA AGTGAGAACT TGAATGCTAA GAATAATACT 
AACTCAGCAT GTTTCTGCAG AAAGGCATTA GAGGGTGTGC CAGTTGGTAA AGCTACAATT 
TTAAATACAG AAAACTTGTC TAGTACACCT GCTCCGAAGT ATTTGAAAAT ATTGCCTTCT 
GGTCTTAAGT ATAATGCAAG ACATCCTTCT ACCAAGGTAA TGAAGCAAAT GGATATAGGT 
GTGTATTTTG GACTACCTCC CAAAAGAAAG GAAGAGAAAT TGCTAGGGGA AAGTGCATTA 
GAAGGGATAA ACTTAAATCC AGTTCCAAGT CCTAATCAAA AGAGGTCCTC GCAGTGCAAG 
AGGAAAGCAG AAAAATCTTT AAGTGATTTA GAATTTGATG CAAGTACTTT ACATGAGAGT 
CAGCTTTCTG TGGAACTTTC TAGTGAGAGG TCACAGCGTC AAAAAAAGAG ATGTAGAAAG 
TCAAATTCAC TGCAGGAAGG AGCGTGTCAG AAGAGATCAG ATCACCTTAT TAATACAGAA 
TCTGAAGCAG TCAATTTAAG TAAAGTCAAA GTCTTCACAA AATCAGCTCA TGGTGGGCTG 
CAAAGGGGCA ACAAGAAAAT CCCAGAGTCA TCTAATGTAG GAGGATCAAG AAAAAAGACA 
TGTCCATTCT ATAAGAAAAT ACCTGGAACC GGCTTTACAG TTGATGCCTT TCAGTATGGC 
GTGGTTGAAG GTTGCACAGC CTATTTTCTC ACACATTTTC ATTCTGATCA TTATGCTGGA 
TTGTCTAAAC ACTTCACATT TCCAGTTTAT TGTAGTGAGA TAACTGGCAA TTTGTTGAAG 
AACAAGCTTC ATGTGCAAGA ACAATATATT CACCCATTGC CACTGGACAC TGAATGTATT 
GTGAATGGTG TCAAAGTTGT TTTGCTTGAT GCCAATCACT GTCCAGGTGC TGTCATGATC 
CTCTTTTATC TTCCTAATGG TACTGTCATA TTACACACGG GAGACTTCAG AGCAGATCCC 
AGCATGGAAC GTTCTCTTCT TGCGGACCAG AAAGTCCATA TGCTGTACTT AGATACCACA 
TATTGTAGCC CAGAATACAC CTTTCCATCT CAGCAAGAGG TTATCCGGTT TGCCATCAAC 
ACTGCCTTTG AGGCTGTAAC TCTAAACCCA CATGCTCTTG TTGTCTGTGG CACTTACTCT 
ATTGGAAAAG AGAAAGTCTT CCTAGCCATT GCTGATGTTT TAGGTTCAAA AGTGGGCATG 
TCCCAGGAAA AATATAAAAC TCTACAGTGC CTCAATATAC CAGAAATTAA TTCACTCATC 
ACTACCGACA TGTGCAGTTC ATTGGTTCAC CTTCTCCCAA TGATGCAAAT TAATTTTAAG 
GGCTTACAGA GTCATTTGAA GAAGTGTGGT GGGAAATACA ATCAGATTTT GGCATTTCGA 
CCTACAGGAT GGACACACTC TAACAAGTTC ACTAGAATAG CAGATGTTAT TCCCCAGACC 
AAAGGAAACA TTTCAATATA TGGAATTCCT TACAGTGAAC ACAGCAGCTA CCTAGAAATG 
AAGCGCTTTG TCCAGTGGCT GAAGCCCCAG AAAATCATAC CTACTGTAAA TGTGGGCACC 
TGGAAATCTA GGAGCACAAT GGAGAAATAT TTTAGAGAGT GGAAATTGGA AGCTGGATAT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RPA activates the XPF-ERCC1 endonuclease to initiate processing of DNA interstrand crosslinks.
The EMBO journal36(14)2047-2060(2017 07)
Abdullah UB,McGouran JF,Brolih S,Ptchelkine D,El-Sagheer AH,Brown T,McHugh PJ

The structures of the SNM1A and SNM1B/Apollo nuclease domains reveal a potential basis for their distinct DNA processing activities.
Nucleic acids research43(22)11047-60(2015 Dec)
Allerston CK,Lee SY,Newman JA,Schofield CJ,McHugh PJ,Gileadi O

Characterization of the human SNM1A and SNM1B/Apollo DNA repair exonucleases.
The Journal of biological chemistry287(31)26254-67(2012 Jul)
Sengerov? B,Allerston CK,Abu M,Lee SY,Hartley J,Kiakos K,Schofield CJ,Hartley JA,Gileadi O,McHugh PJ

Human SNM1A and XPF-ERCC1 collaborate to initiate DNA interstrand cross-link repair.
Genes & development25(17)1859-70(2011 Sep)
Wang AT,Sengerov? B,Cattell E,Inagawa T,Hartley JM,Kiakos K,Burgess-Brown NA,Swift LP,Enzlin JH,Schofield CJ,Gileadi O,Hartley JA,McHugh PJ

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DCLRE1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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