AP5Z1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AP5Z1
Entrez Gene ID	9907
Full Name	adaptor related protein complex 5 zeta 1 subunit
Synonyms	KIAA0415,SPG48,zeta
General protein information	Preferred Names adaptor related protein complex 5 zeta 1 subunit Names AP-5 complex subunit zeta-1 adapter-related protein complex 5 zeta subunit adaptor-related protein complex 5 zeta subunit zeta5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7p22.1
Summary	This gene was identified by genome-wide screen for genes involved in homologous recombination DNA double-strand break repair (HR-DSBR). The encoded protein was found in a complex with other proteins that have a role in HR-DSBR. Knockdown of this gene reduced homologous recombination, and mutations in this gene were found in patients with spastic paraplegia. It was concluded that this gene likely encodes a helicase (PMID:20613862). [provided by RefSeq, Jan 2011].
Disorder MIM:	613653
Disorder Html:	Spastic paraplegia 48, autosomal recessive, 613647 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001364858.1	NP_001351787.1	AP-5 complex subunit zeta-1 isoform 2
XM_017012864.1	XP_016868353.1	AP-5 complex subunit zeta-1 isoform X1
NM_014855.2	NP_055670.1	AP-5 complex subunit zeta-1
NM_014855.3	NP_055670.1	AP-5 complex subunit zeta-1 isoform 1

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	AT3G15160	NP_188134.2
Pan troglodytes (chimpanzee)	AP5Z1	XP_003318329.1
Canis lupus familiaris (dog)	AP5Z1	XP_851709.2
Bos taurus (cattle)	KIAA0415	NP_001192287.1
Rattus norvegicus (Norway rat)	Ap5z1	NP_001032297.3
Gallus gallus (chicken)	AP5Z1	XP_003642161.1
Homo sapiens (human)	AP5Z1	NP_055670.1
Mus musculus (house mouse)	Ap5z1	NP_766313.2
Xenopus tropicalis (tropical clawed frog)	LOC100497560	XP_002942735.2
Danio rerio (zebrafish)	LOC100537934	XP_005164237.1
Oryza sativa (rice)	Os04g0626900	NP_001053950.1

Gene Ontology
Bibliography

Related articles in PubMed

The fifth adaptor protein complex.
Hirst J, Barlow LD, Francisco GC, Sahlender DA, Seaman MN, Dacks JB, Robinson MS
PLoS biology9(10)e1001170(2011 Oct)

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.
S?abicki M, Theis M, Krastev DB, Samsonov S, Mundwiller E, Junqueira M, Paszkowski-Rogacz M, Teyra J, Heninger AK, Poser I, Prieur F, Truchetto J, Confavreux C, Marelli C, Durr A, Camdessanche JP, Brice A, Shevchenko A, Pisabarro MT, Stevanin G, Buchholz F
PLoS biology8(6)e1000408(2010 Jun)

Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epithelium.
Wistow G, Bernstein SL, Ray S, Wyatt MK, Behal A, Touchman JW, Bouffard G, Smith D, Peterson K
Molecular vision8185-95(2002 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following AP5Z1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AP5Z1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20424	NM_014855.3 Latest version!	Homo sapiens adaptor related protein complex 5 subunit zeta 1 (AP5Z1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$566.30 $809.00
OHu97650	NM_001364858.1 Latest version!	Homo sapiens adaptor related protein complex 5 subunit zeta 1 (AP5Z1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu97650	XM_017012864.1 Latest version!	Homo sapiens adaptor related protein complex 5 zeta 1 subunit (AP5Z1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu20424	NM_014855.2	Homo sapiens adaptor related protein complex 5 zeta 1 subunit (AP5Z1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$566.30 $809.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AP5Z1 ( NM_014855.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055670.1 Homo sapiens adaptor related protein complex 5 zeta 1 subunit (AP5Z1), mRNA.

Price & Availability↑

CloneID	OHu20424
Clone ID Related Accession (Same CDS sequence)	NM_014855.2 , NM_014855.3
Accession Version	NM_014855.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	AP-5 complex subunit zeta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI766915.1, CD671081.1, BQ228738.1, BC008841.2, AB007875.1 and AC092610.1. This sequence is a reference standard in the RefSeqGene project. On Jan 25, 2011 this sequence version replaced NM_014855.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCGG CAGGAGCGGA GAGTTTGCTC CACCAGGCCA GGGAGATCCA GGACGAGGAG 
CTGAAGAAGT TCTGTTCCCG GATCTGTAAA CTGCTGCAGG CGGAGGACTT GGGGCCGGAC 
ACCCTCGACT CCCTGCAGAG GCTCTTCCTC ATCATCTCAG CCACGAAGTA CAGCCGGAGG 
CTGGAGAAGA CATGCGTAGA CCTGCTGCAG GCCACCCTCG GCCTGCCTGC ATGCCCCGAG 
CAGCTCCAGG TGCTTTGCGC CGCCATCCTG CGAGAGATGT CCCCCTCTGA CAGCCTCAGC 
CTGGCCTGGG ACCACACGCA GAACAGCCGG CAGCTGAGCC TGGTGGCCTC CGTTCTCTTG 
GCCCAGGGTG ACAGAAACGA GGAGGTCAGA GCCGTGGGCC AGGGCGTGCT ACGAGCGCTG 
GAGAGCCGGC AGCCTGAGGG ACCCAGCCTC AGACACCTCC TCCCCGTCAT GGCCAAGGTC 
GTGGTCCTCA GCCCGGGCAC CCTCCAGGAG GACCAGGCCA CCCTGCTCAG CAAGCGGCTG 
GTCGACTGGC TGCGCTACGC CAGCCTCCAG CAAGGGCTCC CACACTCCGG CGGCTTCTTC 
TCCACGCCCA GGGCCCGGCA GCCGGGCCCC GTCACCGAGG TGGACGGGGC GGTAGCCACA 
GACTTCTTCA CGGTGCTCTC CAGCGGCCAC CGCTTCACAG ACGACCAGTG GCTGAACGTG 
CAGGCCTTCT CTATGCTGCG GGCGTGGCTG CTGCACAGCG GCCCCGAGGG CCCGGGCACC 
CTGGACACAG ATGACAGGTC AGAGCAGGAG GGCTCCACTC TGTCGGTGAT CTCCGCCACC 
TCCTCTGCCG GCCGCCTGCT GCCGCCCCGG GAGCGGCTTC GGGAGGTGGC CTTCGAGTAC 
TGCCAGCGCC TCATTGAGCA AAGTAACCGA CGAGCCCTGA GGAAGGGGGA CTCCGACCTG 
CAGAAAGCTT GCCTGGTGGA GGCCGTGCTG GTGCTGGACG TGCTGTGCCG GCAGGACCCG 
TCCTTCCTGT ACCGAAGTCT CTCCTGCCTG AAGGCCCTGC ACGGGCGGGT GCGCGGGGAC 
CCGGCCTCTG TGCGGGTGCT GCTGCCCCTC GCCCACTTCT TCCTGAGCCA CGGGGAAGCG 
GCTGCAGTGG ACTCGGAAGC CGTCTACCAG CACCTGTTCA CCAGGATCCC GGTGGAGCAG 
TTCCACAGCC CCATGCTGGC CTTTGAATTC ATCCAGTTCT GCAGGGACAA CCTCCACCTG 
TTCAGCGGGC ACCTCAGCAC CCTCAGATTG AGCTTCCCCA ACCTCTTTAA GTTCCTGGCC 
TGGAACAGCC CACCCCTCAC CTCCGAGTTT GTGGCGCTCC TCCCGGCCCT GGTGGACGCT 
GGCACAGCCC TGGAGATGCT GCACGCGCTG CTGGACCTGC CCTGCTTGAC GGCGGTGCTG 
GACCTGCAGC TCAGGTCAGC ACCGGCTGCA TCCGAGAGGC CACTCTGGGA CACCTCTCTC 
AGGGCCCCCA GCTGCCTGGA GGCCTTCCGG GACCCGCAGT TCCAGGGTCT TTTCCAATAC 
CTGCTGCGCC CCAAGGCCAG TGGCGCCACT GAGAGGTTGG CGCCACTCCA CCAGCTGCTG 
CAGCCCATGG CCGGCTGTGC CCGCGTGGCC CAGTGTGCCC AGGCCGTGCC CACGCTGCTG 
CAGGCATTCT TCTCAGCAGT GACCCAGGTG GCTGACGGGT CCCTGATCAA CCAGCTGGCG 
CTGCTGCTCC TGGGCAGGAG CGACTCGCTC TACCCGGCCC CAGGGTACGC TGCCGGTGTG 
CACAGTGTGC TGAGTTCTCA GTTCCTGGCC CTGTGTACGC TGAAACCCTC CCTGGTGGTG 
GAGCTGGCAA GAGACCTGCT GGAGTTCCTG GGCAGCGTGA ATGGTCTCTG CAGCAGGGCG 
AGCCTCGTCA CCAGCGTGGT GTGGGCCATC GGCGAGTACC TGTCGGTGAC CTACGATCGG 
AGGTGCACCG TGGAGCAGAT CAACAAGTTC TTCGAAGCCC TGGAGGCTCT GCTATTCGAG 
GTCACCCAGT GCCGCCCCTC TGCTGCCCTG CCCAGGTGTC CCCCCCAGGT GGTCACCGTG 
CTGATGACCA CGCTGACGAA GCTGGCCTCC CGGAGCCAAG ATCTGATCCC CAGGGCCTCT 
TTATTGCTGT CAAAGATGAG GACCCTGGCT CACAGTCCAG CCACCAGCTC CACGCACAGC 
GAGGAGGGCG CGGAAGCCAT CCGTACCCGG GCCACAGAGC TGCTGACCCT GCTGAAGATG 
CCTAGCGTGG CCCAGTTTGT GCTCACACCC AGCACGGAGG TGTGCAGCCC CCGCTATCAC 
CGCGATGCCA ACACGGCCCT GCCCCTGGCC CTGCGCACGG TCAGCCGGCT GGTGGAGAGG 
GAGGCCGGCC TCATGCCAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055670.1
CDS	86..2509
Translation	MFSAGAESLLHQAREIQDEELKKFCSRICKLLQAEDLGPDTLDSLQRLFLIISATKYSRRLEKTCVDLLQATLGLPACPEQLQVLCAAILREMSPSDSLSLAWDHTQNSRQLSLVASVLLAQGDRNEEVRAVGQGVLRALESRQPEGPSLRHLLPVMAKVVVLSPGTLQEDQATLLSKRLVDWLRYASLQQGLPHSGGFFSTPRARQPGPVTEVDGAVATDFFTVLSSGHRFTDDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASVRVLLPLAHFFLSHGEAAAVDSEAVYQHLFTRIPVEQFHSPMLAFEFIQFCRDNLHLFSGHLSTLRLSFPNLFKFLAWNSPPLTSEFVALLPALVDAGTALEMLHALLDLPCLTAVLDLQLRSAPAASERPLWDTSLRAPSCLEAFRDPQFQGLFQYLLRPKASGATERLAPLHQLLQPMAGCARVAQCAQAVPTLLQAFFSAVTQVADGSLINQLALLLLGRSDSLYPAPGYAAGVHSVLSSQFLALCTLKPSLVVELARDLLEFLGSVNGLCSRASLVTSVVWAIGEYLSVTYDRRCTVEQINKFFEALEALLFEVTQCRPSAALPRCPPQVVTVLMTTLTKLASRSQDLIPRASLLLSKMRTLAHSPATSSTHSEEGAEAIRTRATELLTLLKMPSVAQFVLTPSTEVCSPRYHRDANTALPLALRTVSRLVEREAGLMPG

Target ORF information:

RefSeq Version	NM_014855.2
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 5 zeta 1 subunit (AP5Z1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014855.2

ORF Insert Sequence:

ATGTTCTCGG CAGGAGCGGA GAGTTTGCTC CACCAGGCCA GGGAGATCCA GGACGAGGAG 
CTGAAGAAGT TCTGTTCCCG GATCTGTAAA CTGCTGCAGG CGGAGGACTT GGGGCCGGAC 
ACCCTCGACT CCCTGCAGAG GCTCTTCCTC ATCATCTCAG CCACGAAGTA CAGCCGGAGG 
CTGGAGAAGA CATGCGTAGA CCTGCTGCAG GCCACCCTCG GCCTGCCTGC ATGCCCCGAG 
CAGCTCCAGG TGCTTTGCGC CGCCATCCTG CGAGAGATGT CCCCCTCTGA CAGCCTCAGC 
CTGGCCTGGG ACCACACGCA GAACAGCCGG CAGCTGAGCC TGGTGGCCTC CGTTCTCTTG 
GCCCAGGGTG ACAGAAACGA GGAGGTCAGA GCCGTGGGCC AGGGCGTGCT ACGAGCGCTG 
GAGAGCCGGC AGCCTGAGGG ACCCAGCCTC AGACACCTCC TCCCCGTCAT GGCCAAGGTC 
GTGGTCCTCA GCCCGGGCAC CCTCCAGGAG GACCAGGCCA CCCTGCTCAG CAAGCGGCTG 
GTCGACTGGC TGCGCTACGC CAGCCTCCAG CAAGGGCTCC CACACTCCGG CGGCTTCTTC 
TCCACGCCCA GGGCCCGGCA GCCGGGCCCC GTCACCGAGG TGGACGGGGC GGTAGCCACA 
GACTTCTTCA CGGTGCTCTC CAGCGGCCAC CGCTTCACAG ACGACCAGTG GCTGAACGTG 
CAGGCCTTCT CTATGCTGCG GGCGTGGCTG CTGCACAGCG GCCCCGAGGG CCCGGGCACC 
CTGGACACAG ATGACAGGTC AGAGCAGGAG GGCTCCACTC TGTCGGTGAT CTCCGCCACC 
TCCTCTGCCG GCCGCCTGCT GCCGCCCCGG GAGCGGCTTC GGGAGGTGGC CTTCGAGTAC 
TGCCAGCGCC TCATTGAGCA AAGTAACCGA CGAGCCCTGA GGAAGGGGGA CTCCGACCTG 
CAGAAAGCTT GCCTGGTGGA GGCCGTGCTG GTGCTGGACG TGCTGTGCCG GCAGGACCCG 
TCCTTCCTGT ACCGAAGTCT CTCCTGCCTG AAGGCCCTGC ACGGGCGGGT GCGCGGGGAC 
CCGGCCTCTG TGCGGGTGCT GCTGCCCCTC GCCCACTTCT TCCTGAGCCA CGGGGAAGCG 
GCTGCAGTGG ACTCGGAAGC CGTCTACCAG CACCTGTTCA CCAGGATCCC GGTGGAGCAG 
TTCCACAGCC CCATGCTGGC CTTTGAATTC ATCCAGTTCT GCAGGGACAA CCTCCACCTG 
TTCAGCGGGC ACCTCAGCAC CCTCAGATTG AGCTTCCCCA ACCTCTTTAA GTTCCTGGCC 
TGGAACAGCC CACCCCTCAC CTCCGAGTTT GTGGCGCTCC TCCCGGCCCT GGTGGACGCT 
GGCACAGCCC TGGAGATGCT GCACGCGCTG CTGGACCTGC CCTGCTTGAC GGCGGTGCTG 
GACCTGCAGC TCAGGTCAGC ACCGGCTGCA TCCGAGAGGC CACTCTGGGA CACCTCTCTC 
AGGGCCCCCA GCTGCCTGGA GGCCTTCCGG GACCCGCAGT TCCAGGGTCT TTTCCAATAC 
CTGCTGCGCC CCAAGGCCAG TGGCGCCACT GAGAGGTTGG CGCCACTCCA CCAGCTGCTG 
CAGCCCATGG CCGGCTGTGC CCGCGTGGCC CAGTGTGCCC AGGCCGTGCC CACGCTGCTG 
CAGGCATTCT TCTCAGCAGT GACCCAGGTG GCTGACGGGT CCCTGATCAA CCAGCTGGCG 
CTGCTGCTCC TGGGCAGGAG CGACTCGCTC TACCCGGCCC CAGGGTACGC TGCCGGTGTG 
CACAGTGTGC TGAGTTCTCA GTTCCTGGCC CTGTGTACGC TGAAACCCTC CCTGGTGGTG 
GAGCTGGCAA GAGACCTGCT GGAGTTCCTG GGCAGCGTGA ATGGTCTCTG CAGCAGGGCG 
AGCCTCGTCA CCAGCGTGGT GTGGGCCATC GGCGAGTACC TGTCGGTGAC CTACGATCGG 
AGGTGCACCG TGGAGCAGAT CAACAAGTTC TTCGAAGCCC TGGAGGCTCT GCTATTCGAG 
GTCACCCAGT GCCGCCCCTC TGCTGCCCTG CCCAGGTGTC CCCCCCAGGT GGTCACCGTG 
CTGATGACCA CGCTGACGAA GCTGGCCTCC CGGAGCCAAG ATCTGATCCC CAGGGCCTCT 
TTATTGCTGT CAAAGATGAG GACCCTGGCT CACAGTCCAG CCACCAGCTC CACGCACAGC 
GAGGAGGGCG CGGAAGCCAT CCGTACCCGG GCCACAGAGC TGCTGACCCT GCTGAAGATG 
CCTAGCGTGG CCCAGTTTGT GCTCACACCC AGCACGGAGG TGTGCAGCCC CCGCTATCAC 
CGCGATGCCA ACACGGCCCT GCCCCTGGCC CTGCGCACGG TCAGCCGGCT GGTGGAGAGG 
GAGGCCGGCC TCATGCCAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP5Z1 ( NM_014855.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055670.1 Homo sapiens adaptor related protein complex 5 subunit zeta 1 (AP5Z1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20424
Clone ID Related Accession (Same CDS sequence)	NM_014855.2 , NM_014855.3
Accession Version	NM_014855.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2424bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	AP-5 complex subunit zeta-1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY145300.1, BI766915.1, CD671081.1, BQ228738.1, BC008841.2, AB007875.1, AC092610.1 and AI927641.1. This sequence is a reference standard in the RefSeqGene project. On Jul 11, 2018 this sequence version replaced NM_014855.2. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.81952.1, SRR1660805.176643.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000649063.2/ ENSP00000497815.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCTCGG CAGGAGCGGA GAGTTTGCTC CACCAGGCCA GGGAGATCCA GGACGAGGAG 
CTGAAGAAGT TCTGTTCCCG GATCTGTAAA CTGCTGCAGG CGGAGGACTT GGGGCCGGAC 
ACCCTCGACT CCCTGCAGAG GCTCTTCCTC ATCATCTCAG CCACGAAGTA CAGCCGGAGG 
CTGGAGAAGA CATGCGTAGA CCTGCTGCAG GCCACCCTCG GCCTGCCTGC ATGCCCCGAG 
CAGCTCCAGG TGCTTTGCGC CGCCATCCTG CGAGAGATGT CCCCCTCTGA CAGCCTCAGC 
CTGGCCTGGG ACCACACGCA GAACAGCCGG CAGCTGAGCC TGGTGGCCTC CGTTCTCTTG 
GCCCAGGGTG ACAGAAACGA GGAGGTCAGA GCCGTGGGCC AGGGCGTGCT ACGAGCGCTG 
GAGAGCCGGC AGCCTGAGGG ACCCAGCCTC AGACACCTCC TCCCCGTCAT GGCCAAGGTC 
GTGGTCCTCA GCCCGGGCAC CCTCCAGGAG GACCAGGCCA CCCTGCTCAG CAAGCGGCTG 
GTCGACTGGC TGCGCTACGC CAGCCTCCAG CAAGGGCTCC CACACTCCGG CGGCTTCTTC 
TCCACGCCCA GGGCCCGGCA GCCGGGCCCC GTCACCGAGG TGGACGGGGC GGTAGCCACA 
GACTTCTTCA CGGTGCTCTC CAGCGGCCAC CGCTTCACAG ACGACCAGTG GCTGAACGTG 
CAGGCCTTCT CTATGCTGCG GGCGTGGCTG CTGCACAGCG GCCCCGAGGG CCCGGGCACC 
CTGGACACAG ATGACAGGTC AGAGCAGGAG GGCTCCACTC TGTCGGTGAT CTCCGCCACC 
TCCTCTGCCG GCCGCCTGCT GCCGCCCCGG GAGCGGCTTC GGGAGGTGGC CTTCGAGTAC 
TGCCAGCGCC TCATTGAGCA AAGTAACCGA CGAGCCCTGA GGAAGGGGGA CTCCGACCTG 
CAGAAAGCTT GCCTGGTGGA GGCCGTGCTG GTGCTGGACG TGCTGTGCCG GCAGGACCCG 
TCCTTCCTGT ACCGAAGTCT CTCCTGCCTG AAGGCCCTGC ACGGGCGGGT GCGCGGGGAC 
CCGGCCTCTG TGCGGGTGCT GCTGCCCCTC GCCCACTTCT TCCTGAGCCA CGGGGAAGCG 
GCTGCAGTGG ACTCGGAAGC CGTCTACCAG CACCTGTTCA CCAGGATCCC GGTGGAGCAG 
TTCCACAGCC CCATGCTGGC CTTTGAATTC ATCCAGTTCT GCAGGGACAA CCTCCACCTG 
TTCAGCGGGC ACCTCAGCAC CCTCAGATTG AGCTTCCCCA ACCTCTTTAA GTTCCTGGCC 
TGGAACAGCC CACCCCTCAC CTCCGAGTTT GTGGCGCTCC TCCCGGCCCT GGTGGACGCT 
GGCACAGCCC TGGAGATGCT GCACGCGCTG CTGGACCTGC CCTGCTTGAC GGCGGTGCTG 
GACCTGCAGC TCAGGTCAGC ACCGGCTGCA TCCGAGAGGC CACTCTGGGA CACCTCTCTC 
AGGGCCCCCA GCTGCCTGGA GGCCTTCCGG GACCCGCAGT TCCAGGGTCT TTTCCAATAC 
CTGCTGCGCC CCAAGGCCAG TGGCGCCACT GAGAGGTTGG CGCCACTCCA CCAGCTGCTG 
CAGCCCATGG CCGGCTGTGC CCGCGTGGCC CAGTGTGCCC AGGCCGTGCC CACGCTGCTG 
CAGGCATTCT TCTCAGCAGT GACCCAGGTG GCTGACGGGT CCCTGATCAA CCAGCTGGCG 
CTGCTGCTCC TGGGCAGGAG CGACTCGCTC TACCCGGCCC CAGGGTACGC TGCCGGTGTG 
CACAGTGTGC TGAGTTCTCA GTTCCTGGCC CTGTGTACGC TGAAACCCTC CCTGGTGGTG 
GAGCTGGCAA GAGACCTGCT GGAGTTCCTG GGCAGCGTGA ATGGTCTCTG CAGCAGGGCG 
AGCCTCGTCA CCAGCGTGGT GTGGGCCATC GGCGAGTACC TGTCGGTGAC CTACGATCGG 
AGGTGCACCG TGGAGCAGAT CAACAAGTTC TTCGAAGCCC TGGAGGCTCT GCTATTCGAG 
GTCACCCAGT GCCGCCCCTC TGCTGCCCTG CCCAGGTGTC CCCCCCAGGT GGTCACCGTG 
CTGATGACCA CGCTGACGAA GCTGGCCTCC CGGAGCCAAG ATCTGATCCC CAGGGCCTCT 
TTATTGCTGT CAAAGATGAG GACCCTGGCT CACAGTCCAG CCACCAGCTC CACGCACAGC 
GAGGAGGGCG CGGAAGCCAT CCGTACCCGG GCCACAGAGC TGCTGACCCT GCTGAAGATG 
CCTAGCGTGG CCCAGTTTGT GCTCACACCC AGCACGGAGG TGTGCAGCCC CCGCTATCAC 
CGCGATGCCA ACACGGCCCT GCCCCTGGCC CTGCGCACGG TCAGCCGGCT GGTGGAGAGG 
GAGGCCGGCC TCATGCCAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055670.1
CDS	94..2517
Translation	MFSAGAESLLHQAREIQDEELKKFCSRICKLLQAEDLGPDTLDSLQRLFLIISATKYSRRLEKTCVDLLQATLGLPACPEQLQVLCAAILREMSPSDSLSLAWDHTQNSRQLSLVASVLLAQGDRNEEVRAVGQGVLRALESRQPEGPSLRHLLPVMAKVVVLSPGTLQEDQATLLSKRLVDWLRYASLQQGLPHSGGFFSTPRARQPGPVTEVDGAVATDFFTVLSSGHRFTDDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASVRVLLPLAHFFLSHGEAAAVDSEAVYQHLFTRIPVEQFHSPMLAFEFIQFCRDNLHLFSGHLSTLRLSFPNLFKFLAWNSPPLTSEFVALLPALVDAGTALEMLHALLDLPCLTAVLDLQLRSAPAASERPLWDTSLRAPSCLEAFRDPQFQGLFQYLLRPKASGATERLAPLHQLLQPMAGCARVAQCAQAVPTLLQAFFSAVTQVADGSLINQLALLLLGRSDSLYPAPGYAAGVHSVLSSQFLALCTLKPSLVVELARDLLEFLGSVNGLCSRASLVTSVVWAIGEYLSVTYDRRCTVEQINKFFEALEALLFEVTQCRPSAALPRCPPQVVTVLMTTLTKLASRSQDLIPRASLLLSKMRTLAHSPATSSTHSEEGAEAIRTRATELLTLLKMPSVAQFVLTPSTEVCSPRYHRDANTALPLALRTVSRLVEREAGLMPG

Target ORF information:

RefSeq Version	NM_014855.3
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 5 subunit zeta 1 (AP5Z1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014855.3

ORF Insert Sequence:

ATGTTCTCGG CAGGAGCGGA GAGTTTGCTC CACCAGGCCA GGGAGATCCA GGACGAGGAG 
CTGAAGAAGT TCTGTTCCCG GATCTGTAAA CTGCTGCAGG CGGAGGACTT GGGGCCGGAC 
ACCCTCGACT CCCTGCAGAG GCTCTTCCTC ATCATCTCAG CCACGAAGTA CAGCCGGAGG 
CTGGAGAAGA CATGCGTAGA CCTGCTGCAG GCCACCCTCG GCCTGCCTGC ATGCCCCGAG 
CAGCTCCAGG TGCTTTGCGC CGCCATCCTG CGAGAGATGT CCCCCTCTGA CAGCCTCAGC 
CTGGCCTGGG ACCACACGCA GAACAGCCGG CAGCTGAGCC TGGTGGCCTC CGTTCTCTTG 
GCCCAGGGTG ACAGAAACGA GGAGGTCAGA GCCGTGGGCC AGGGCGTGCT ACGAGCGCTG 
GAGAGCCGGC AGCCTGAGGG ACCCAGCCTC AGACACCTCC TCCCCGTCAT GGCCAAGGTC 
GTGGTCCTCA GCCCGGGCAC CCTCCAGGAG GACCAGGCCA CCCTGCTCAG CAAGCGGCTG 
GTCGACTGGC TGCGCTACGC CAGCCTCCAG CAAGGGCTCC CACACTCCGG CGGCTTCTTC 
TCCACGCCCA GGGCCCGGCA GCCGGGCCCC GTCACCGAGG TGGACGGGGC GGTAGCCACA 
GACTTCTTCA CGGTGCTCTC CAGCGGCCAC CGCTTCACAG ACGACCAGTG GCTGAACGTG 
CAGGCCTTCT CTATGCTGCG GGCGTGGCTG CTGCACAGCG GCCCCGAGGG CCCGGGCACC 
CTGGACACAG ATGACAGGTC AGAGCAGGAG GGCTCCACTC TGTCGGTGAT CTCCGCCACC 
TCCTCTGCCG GCCGCCTGCT GCCGCCCCGG GAGCGGCTTC GGGAGGTGGC CTTCGAGTAC 
TGCCAGCGCC TCATTGAGCA AAGTAACCGA CGAGCCCTGA GGAAGGGGGA CTCCGACCTG 
CAGAAAGCTT GCCTGGTGGA GGCCGTGCTG GTGCTGGACG TGCTGTGCCG GCAGGACCCG 
TCCTTCCTGT ACCGAAGTCT CTCCTGCCTG AAGGCCCTGC ACGGGCGGGT GCGCGGGGAC 
CCGGCCTCTG TGCGGGTGCT GCTGCCCCTC GCCCACTTCT TCCTGAGCCA CGGGGAAGCG 
GCTGCAGTGG ACTCGGAAGC CGTCTACCAG CACCTGTTCA CCAGGATCCC GGTGGAGCAG 
TTCCACAGCC CCATGCTGGC CTTTGAATTC ATCCAGTTCT GCAGGGACAA CCTCCACCTG 
TTCAGCGGGC ACCTCAGCAC CCTCAGATTG AGCTTCCCCA ACCTCTTTAA GTTCCTGGCC 
TGGAACAGCC CACCCCTCAC CTCCGAGTTT GTGGCGCTCC TCCCGGCCCT GGTGGACGCT 
GGCACAGCCC TGGAGATGCT GCACGCGCTG CTGGACCTGC CCTGCTTGAC GGCGGTGCTG 
GACCTGCAGC TCAGGTCAGC ACCGGCTGCA TCCGAGAGGC CACTCTGGGA CACCTCTCTC 
AGGGCCCCCA GCTGCCTGGA GGCCTTCCGG GACCCGCAGT TCCAGGGTCT TTTCCAATAC 
CTGCTGCGCC CCAAGGCCAG TGGCGCCACT GAGAGGTTGG CGCCACTCCA CCAGCTGCTG 
CAGCCCATGG CCGGCTGTGC CCGCGTGGCC CAGTGTGCCC AGGCCGTGCC CACGCTGCTG 
CAGGCATTCT TCTCAGCAGT GACCCAGGTG GCTGACGGGT CCCTGATCAA CCAGCTGGCG 
CTGCTGCTCC TGGGCAGGAG CGACTCGCTC TACCCGGCCC CAGGGTACGC TGCCGGTGTG 
CACAGTGTGC TGAGTTCTCA GTTCCTGGCC CTGTGTACGC TGAAACCCTC CCTGGTGGTG 
GAGCTGGCAA GAGACCTGCT GGAGTTCCTG GGCAGCGTGA ATGGTCTCTG CAGCAGGGCG 
AGCCTCGTCA CCAGCGTGGT GTGGGCCATC GGCGAGTACC TGTCGGTGAC CTACGATCGG 
AGGTGCACCG TGGAGCAGAT CAACAAGTTC TTCGAAGCCC TGGAGGCTCT GCTATTCGAG 
GTCACCCAGT GCCGCCCCTC TGCTGCCCTG CCCAGGTGTC CCCCCCAGGT GGTCACCGTG 
CTGATGACCA CGCTGACGAA GCTGGCCTCC CGGAGCCAAG ATCTGATCCC CAGGGCCTCT 
TTATTGCTGT CAAAGATGAG GACCCTGGCT CACAGTCCAG CCACCAGCTC CACGCACAGC 
GAGGAGGGCG CGGAAGCCAT CCGTACCCGG GCCACAGAGC TGCTGACCCT GCTGAAGATG 
CCTAGCGTGG CCCAGTTTGT GCTCACACCC AGCACGGAGG TGTGCAGCCC CCGCTATCAC 
CGCGATGCCA ACACGGCCCT GCCCCTGGCC CTGCGCACGG TCAGCCGGCT GGTGGAGAGG 
GAGGCCGGCC TCATGCCAGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP5Z1 ( NM_001364858.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351787.1 Homo sapiens adaptor related protein complex 5 subunit zeta 1 (AP5Z1), transcript variant 2, mRNA.

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CloneID	OHu97650
Clone ID Related Accession (Same CDS sequence)	NM_001364858.1 , XM_017012864.1
Accession Version	NM_001364858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	AP-5 complex subunit zeta-1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092610.1. On Jul 11, 2018 this sequence version replaced XM_017012864.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.267196.1, SRR1803614.226556.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAAGG TCGTGGTCCT CAGCCCGGGC ACCCTCCAGG AGGACCAGGC CACCCTGCTC 
AGCAAGCGGC TGGTCGACTG GCTGCGCTAC GCCAGCCTCC AGCAAGGGCT CCCACACTCC 
GGCGGCTTCT TCTCCACGCC CAGGGCCCGG CAGCCGGGCC CCGTCACCGA GGTGGACGGG 
GCGGTAGCCA CAGACTTCTT CACGGTGCTC TCCAGCGGCC ACCGCTTCAC AGACGACCAG 
TGGCTGAACG TGCAGGCCTT CTCTATGCTG CGGGCGTGGC TGCTGCACAG CGGCCCCGAG 
GGCCCGGGCA CCCTGGACAC AGATGACAGG TCAGAGCAGG AGGGCTCCAC TCTGTCGGTG 
ATCTCCGCCA CCTCCTCTGC CGGCCGCCTG CTGCCGCCCC GGGAGCGGCT TCGGGAGGTG 
GCCTTCGAGT ACTGCCAGCG CCTCATTGAG CAAAGTAACC GACGAGCCCT GAGGAAGGGG 
GACTCCGACC TGCAGAAAGC TTGCCTGGTG GAGGCCGTGC TGGTGCTGGA CGTGCTGTGC 
CGGCAGGACC CGTCCTTCCT GTACCGAAGT CTCTCCTGCC TGAAGGCCCT GCACGGGCGG 
GTGCGCGGGG ACCCGGCCTC TGTGCGGGTG CTGCTGCCCC TCGCCCACTT CTTCCTGAGC 
CACGGGGAAG CGGCTGCAGT GGACTCGGAA GCCGTCTACC AGCACCTGTT CACCAGGATC 
CCGGTGGAGC AGTTCCACAG CCCCATGCTG GCCTTTGAAT TCATCCAGTT CTGCAGGGAC 
AACCTCCACC TGTTCAGCGG GCACCTCAGC ACCCTCAGAT TGAGCTTCCC CAACCTCTTT 
AAGTTCCTGG CCTGGAACAG CCCACCCCTC ACCTCCGAGT TTGTGGCGCT CCTCCCGGCC 
CTGGTGGACG CTGGCACAGC CCTGGAGATG CTGCACGCGC TGCTGGACCT GCCCTGCTTG 
ACGGCGGTGC TGGACCTGCA GCTCAGGTCA GCACCGGCTG CATCCGAGAG GCCACTCTGG 
GACACCTCTC TCAGGGCCCC CAGCTGCCTG GAGGCCTTCC GGGACCCGCA GTTCCAGGGT 
CTTTTCCAAT ACCTGCTGCG CCCCAAGGCC AGTGGCGCCA CTGAGAGGTT GGCGCCACTC 
CACCAGCTGC TGCAGCCCAT GGCCGGCTGT GCCCGCGTGG CCCAGTGTGC CCAGGCCGTG 
CCCACGCTGC TGCAGGCATT CTTCTCAGCA GTGACCCAGG TGGCTGACGG GTCCCTGATC 
AACCAGCTGG CGCTGCTGCT CCTGGGCAGG AGCGACTCGC TCTACCCGGC CCCAGGGTAC 
GCTGCCGGTG TGCACAGTGT GCTGAGTTCT CAGTTCCTGG CCCTGTGTAC GCTGAAACCC 
TCCCTGGTGG TGGAGCTGGC AAGAGACCTG CTGGAGTTCC TGGGCAGCGT GAATGGTCTC 
TGCAGCAGGG CGAGCCTCGT CACCAGCGTG GTGTGGGCCA TCGGCGAGTA CCTGTCGGTG 
ACCTACGATC GGAGGTGCAC CGTGGAGCAG ATCAACAAGT TCTTCGAAGC CCTGGAGGCT 
CTGCTATTCG AGGTCACCCA GTGCCGCCCC TCTGCTGCCC TGCCCAGGTG TCCCCCCCAG 
GTGGTCACCG TGCTGATGAC CACGCTGACG AAGCTGGCCT CCCGGAGCCA AGATCTGATC 
CCCAGGGCCT CTTTATTGCT GTCAAAGATG AGGACCCTGG CTCACAGTCC AGCCACCAGC 
TCCACGCACA GCGAGGAGGG CGCGGAAGCC ATCCGTACCC GGGCCACAGA GCTGCTGACC 
CTGCTGAAGA TGCCTAGCGT GGCCCAGTTT GTGCTCACAC CCAGCACGGA GGTGTGCAGC 
CCCCGCTATC ACCGCGATGC CAACACGGCC CTGCCCCTGG CCCTGCGCAC GGTCAGCCGG 
CTGGTGGAGA GGGAGGCCGG CCTCATGCCA GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351787.1
CDS	375..2330
Translation	MAKVVVLSPGTLQEDQATLLSKRLVDWLRYASLQQGLPHSGGFFSTPRARQPGPVTEVDGAVATDFFTVLSSGHRFTDDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASVRVLLPLAHFFLSHGEAAAVDSEAVYQHLFTRIPVEQFHSPMLAFEFIQFCRDNLHLFSGHLSTLRLSFPNLFKFLAWNSPPLTSEFVALLPALVDAGTALEMLHALLDLPCLTAVLDLQLRSAPAASERPLWDTSLRAPSCLEAFRDPQFQGLFQYLLRPKASGATERLAPLHQLLQPMAGCARVAQCAQAVPTLLQAFFSAVTQVADGSLINQLALLLLGRSDSLYPAPGYAAGVHSVLSSQFLALCTLKPSLVVELARDLLEFLGSVNGLCSRASLVTSVVWAIGEYLSVTYDRRCTVEQINKFFEALEALLFEVTQCRPSAALPRCPPQVVTVLMTTLTKLASRSQDLIPRASLLLSKMRTLAHSPATSSTHSEEGAEAIRTRATELLTLLKMPSVAQFVLTPSTEVCSPRYHRDANTALPLALRTVSRLVEREAGLMPG

Target ORF information:

RefSeq Version	NM_001364858.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 5 subunit zeta 1 (AP5Z1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364858.1

ORF Insert Sequence:

ATGGCCAAGG TCGTGGTCCT CAGCCCGGGC ACCCTCCAGG AGGACCAGGC CACCCTGCTC 
AGCAAGCGGC TGGTCGACTG GCTGCGCTAC GCCAGCCTCC AGCAAGGGCT CCCACACTCC 
GGCGGCTTCT TCTCCACGCC CAGGGCCCGG CAGCCGGGCC CCGTCACCGA GGTGGACGGG 
GCGGTAGCCA CAGACTTCTT CACGGTGCTC TCCAGCGGCC ACCGCTTCAC AGACGACCAG 
TGGCTGAACG TGCAGGCCTT CTCTATGCTG CGGGCGTGGC TGCTGCACAG CGGCCCCGAG 
GGCCCGGGCA CCCTGGACAC AGATGACAGG TCAGAGCAGG AGGGCTCCAC TCTGTCGGTG 
ATCTCCGCCA CCTCCTCTGC CGGCCGCCTG CTGCCGCCCC GGGAGCGGCT TCGGGAGGTG 
GCCTTCGAGT ACTGCCAGCG CCTCATTGAG CAAAGTAACC GACGAGCCCT GAGGAAGGGG 
GACTCCGACC TGCAGAAAGC TTGCCTGGTG GAGGCCGTGC TGGTGCTGGA CGTGCTGTGC 
CGGCAGGACC CGTCCTTCCT GTACCGAAGT CTCTCCTGCC TGAAGGCCCT GCACGGGCGG 
GTGCGCGGGG ACCCGGCCTC TGTGCGGGTG CTGCTGCCCC TCGCCCACTT CTTCCTGAGC 
CACGGGGAAG CGGCTGCAGT GGACTCGGAA GCCGTCTACC AGCACCTGTT CACCAGGATC 
CCGGTGGAGC AGTTCCACAG CCCCATGCTG GCCTTTGAAT TCATCCAGTT CTGCAGGGAC 
AACCTCCACC TGTTCAGCGG GCACCTCAGC ACCCTCAGAT TGAGCTTCCC CAACCTCTTT 
AAGTTCCTGG CCTGGAACAG CCCACCCCTC ACCTCCGAGT TTGTGGCGCT CCTCCCGGCC 
CTGGTGGACG CTGGCACAGC CCTGGAGATG CTGCACGCGC TGCTGGACCT GCCCTGCTTG 
ACGGCGGTGC TGGACCTGCA GCTCAGGTCA GCACCGGCTG CATCCGAGAG GCCACTCTGG 
GACACCTCTC TCAGGGCCCC CAGCTGCCTG GAGGCCTTCC GGGACCCGCA GTTCCAGGGT 
CTTTTCCAAT ACCTGCTGCG CCCCAAGGCC AGTGGCGCCA CTGAGAGGTT GGCGCCACTC 
CACCAGCTGC TGCAGCCCAT GGCCGGCTGT GCCCGCGTGG CCCAGTGTGC CCAGGCCGTG 
CCCACGCTGC TGCAGGCATT CTTCTCAGCA GTGACCCAGG TGGCTGACGG GTCCCTGATC 
AACCAGCTGG CGCTGCTGCT CCTGGGCAGG AGCGACTCGC TCTACCCGGC CCCAGGGTAC 
GCTGCCGGTG TGCACAGTGT GCTGAGTTCT CAGTTCCTGG CCCTGTGTAC GCTGAAACCC 
TCCCTGGTGG TGGAGCTGGC AAGAGACCTG CTGGAGTTCC TGGGCAGCGT GAATGGTCTC 
TGCAGCAGGG CGAGCCTCGT CACCAGCGTG GTGTGGGCCA TCGGCGAGTA CCTGTCGGTG 
ACCTACGATC GGAGGTGCAC CGTGGAGCAG ATCAACAAGT TCTTCGAAGC CCTGGAGGCT 
CTGCTATTCG AGGTCACCCA GTGCCGCCCC TCTGCTGCCC TGCCCAGGTG TCCCCCCCAG 
GTGGTCACCG TGCTGATGAC CACGCTGACG AAGCTGGCCT CCCGGAGCCA AGATCTGATC 
CCCAGGGCCT CTTTATTGCT GTCAAAGATG AGGACCCTGG CTCACAGTCC AGCCACCAGC 
TCCACGCACA GCGAGGAGGG CGCGGAAGCC ATCCGTACCC GGGCCACAGA GCTGCTGACC 
CTGCTGAAGA TGCCTAGCGT GGCCCAGTTT GTGCTCACAC CCAGCACGGA GGTGTGCAGC 
CCCCGCTATC ACCGCGATGC CAACACGGCC CTGCCCCTGG CCCTGCGCAC GGTCAGCCGG 
CTGGTGGAGA GGGAGGCCGG CCTCATGCCA GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AP5Z1 ( XM_017012864.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868353.1 Homo sapiens adaptor related protein complex 5 zeta 1 subunit (AP5Z1), transcript variant X1, mRNA.

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CloneID	OHu97650
Clone ID Related Accession (Same CDS sequence)	NM_001364858.1 , XM_017012864.1
Accession Version	XM_017012864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	AP-5 complex subunit zeta-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG TCGTGGTCCT CAGCCCGGGC ACCCTCCAGG AGGACCAGGC CACCCTGCTC 
AGCAAGCGGC TGGTCGACTG GCTGCGCTAC GCCAGCCTCC AGCAAGGGCT CCCACACTCC 
GGCGGCTTCT TCTCCACGCC CAGGGCCCGG CAGCCGGGCC CCGTCACCGA GGTGGACGGG 
GCGGTAGCCA CAGACTTCTT CACGGTGCTC TCCAGCGGCC ACCGCTTCAC AGACGACCAG 
TGGCTGAACG TGCAGGCCTT CTCTATGCTG CGGGCGTGGC TGCTGCACAG CGGCCCCGAG 
GGCCCGGGCA CCCTGGACAC AGATGACAGG TCAGAGCAGG AGGGCTCCAC TCTGTCGGTG 
ATCTCCGCCA CCTCCTCTGC CGGCCGCCTG CTGCCGCCCC GGGAGCGGCT TCGGGAGGTG 
GCCTTCGAGT ACTGCCAGCG CCTCATTGAG CAAAGTAACC GACGAGCCCT GAGGAAGGGG 
GACTCCGACC TGCAGAAAGC TTGCCTGGTG GAGGCCGTGC TGGTGCTGGA CGTGCTGTGC 
CGGCAGGACC CGTCCTTCCT GTACCGAAGT CTCTCCTGCC TGAAGGCCCT GCACGGGCGG 
GTGCGCGGGG ACCCGGCCTC TGTGCGGGTG CTGCTGCCCC TCGCCCACTT CTTCCTGAGC 
CACGGGGAAG CGGCTGCAGT GGACTCGGAA GCCGTCTACC AGCACCTGTT CACCAGGATC 
CCGGTGGAGC AGTTCCACAG CCCCATGCTG GCCTTTGAAT TCATCCAGTT CTGCAGGGAC 
AACCTCCACC TGTTCAGCGG GCACCTCAGC ACCCTCAGAT TGAGCTTCCC CAACCTCTTT 
AAGTTCCTGG CCTGGAACAG CCCACCCCTC ACCTCCGAGT TTGTGGCGCT CCTCCCGGCC 
CTGGTGGACG CTGGCACAGC CCTGGAGATG CTGCACGCGC TGCTGGACCT GCCCTGCTTG 
ACGGCGGTGC TGGACCTGCA GCTCAGGTCA GCACCGGCTG CATCCGAGAG GCCACTCTGG 
GACACCTCTC TCAGGGCCCC CAGCTGCCTG GAGGCCTTCC GGGACCCGCA GTTCCAGGGT 
CTTTTCCAAT ACCTGCTGCG CCCCAAGGCC AGTGGCGCCA CTGAGAGGTT GGCGCCACTC 
CACCAGCTGC TGCAGCCCAT GGCCGGCTGT GCCCGCGTGG CCCAGTGTGC CCAGGCCGTG 
CCCACGCTGC TGCAGGCATT CTTCTCAGCA GTGACCCAGG TGGCTGACGG GTCCCTGATC 
AACCAGCTGG CGCTGCTGCT CCTGGGCAGG AGCGACTCGC TCTACCCGGC CCCAGGGTAC 
GCTGCCGGTG TGCACAGTGT GCTGAGTTCT CAGTTCCTGG CCCTGTGTAC GCTGAAACCC 
TCCCTGGTGG TGGAGCTGGC AAGAGACCTG CTGGAGTTCC TGGGCAGCGT GAATGGTCTC 
TGCAGCAGGG CGAGCCTCGT CACCAGCGTG GTGTGGGCCA TCGGCGAGTA CCTGTCGGTG 
ACCTACGATC GGAGGTGCAC CGTGGAGCAG ATCAACAAGT TCTTCGAAGC CCTGGAGGCT 
CTGCTATTCG AGGTCACCCA GTGCCGCCCC TCTGCTGCCC TGCCCAGGTG TCCCCCCCAG 
GTGGTCACCG TGCTGATGAC CACGCTGACG AAGCTGGCCT CCCGGAGCCA AGATCTGATC 
CCCAGGGCCT CTTTATTGCT GTCAAAGATG AGGACCCTGG CTCACAGTCC AGCCACCAGC 
TCCACGCACA GCGAGGAGGG CGCGGAAGCC ATCCGTACCC GGGCCACAGA GCTGCTGACC 
CTGCTGAAGA TGCCTAGCGT GGCCCAGTTT GTGCTCACAC CCAGCACGGA GGTGTGCAGC 
CCCCGCTATC ACCGCGATGC CAACACGGCC CTGCCCCTGG CCCTGCGCAC GGTCAGCCGG 
CTGGTGGAGA GGGAGGCCGG CCTCATGCCA GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868353.1
CDS	381..2336
Translation	MAKVVVLSPGTLQEDQATLLSKRLVDWLRYASLQQGLPHSGGFFSTPRARQPGPVTEVDGAVATDFFTVLSSGHRFTDDQWLNVQAFSMLRAWLLHSGPEGPGTLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASVRVLLPLAHFFLSHGEAAAVDSEAVYQHLFTRIPVEQFHSPMLAFEFIQFCRDNLHLFSGHLSTLRLSFPNLFKFLAWNSPPLTSEFVALLPALVDAGTALEMLHALLDLPCLTAVLDLQLRSAPAASERPLWDTSLRAPSCLEAFRDPQFQGLFQYLLRPKASGATERLAPLHQLLQPMAGCARVAQCAQAVPTLLQAFFSAVTQVADGSLINQLALLLLGRSDSLYPAPGYAAGVHSVLSSQFLALCTLKPSLVVELARDLLEFLGSVNGLCSRASLVTSVVWAIGEYLSVTYDRRCTVEQINKFFEALEALLFEVTQCRPSAALPRCPPQVVTVLMTTLTKLASRSQDLIPRASLLLSKMRTLAHSPATSSTHSEEGAEAIRTRATELLTLLKMPSVAQFVLTPSTEVCSPRYHRDANTALPLALRTVSRLVEREAGLMPG

Target ORF information:

RefSeq Version	XM_017012864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens adaptor related protein complex 5 zeta 1 subunit (AP5Z1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012864.1

ORF Insert Sequence:

ATGGCCAAGG TCGTGGTCCT CAGCCCGGGC ACCCTCCAGG AGGACCAGGC CACCCTGCTC 
AGCAAGCGGC TGGTCGACTG GCTGCGCTAC GCCAGCCTCC AGCAAGGGCT CCCACACTCC 
GGCGGCTTCT TCTCCACGCC CAGGGCCCGG CAGCCGGGCC CCGTCACCGA GGTGGACGGG 
GCGGTAGCCA CAGACTTCTT CACGGTGCTC TCCAGCGGCC ACCGCTTCAC AGACGACCAG 
TGGCTGAACG TGCAGGCCTT CTCTATGCTG CGGGCGTGGC TGCTGCACAG CGGCCCCGAG 
GGCCCGGGCA CCCTGGACAC AGATGACAGG TCAGAGCAGG AGGGCTCCAC TCTGTCGGTG 
ATCTCCGCCA CCTCCTCTGC CGGCCGCCTG CTGCCGCCCC GGGAGCGGCT TCGGGAGGTG 
GCCTTCGAGT ACTGCCAGCG CCTCATTGAG CAAAGTAACC GACGAGCCCT GAGGAAGGGG 
GACTCCGACC TGCAGAAAGC TTGCCTGGTG GAGGCCGTGC TGGTGCTGGA CGTGCTGTGC 
CGGCAGGACC CGTCCTTCCT GTACCGAAGT CTCTCCTGCC TGAAGGCCCT GCACGGGCGG 
GTGCGCGGGG ACCCGGCCTC TGTGCGGGTG CTGCTGCCCC TCGCCCACTT CTTCCTGAGC 
CACGGGGAAG CGGCTGCAGT GGACTCGGAA GCCGTCTACC AGCACCTGTT CACCAGGATC 
CCGGTGGAGC AGTTCCACAG CCCCATGCTG GCCTTTGAAT TCATCCAGTT CTGCAGGGAC 
AACCTCCACC TGTTCAGCGG GCACCTCAGC ACCCTCAGAT TGAGCTTCCC CAACCTCTTT 
AAGTTCCTGG CCTGGAACAG CCCACCCCTC ACCTCCGAGT TTGTGGCGCT CCTCCCGGCC 
CTGGTGGACG CTGGCACAGC CCTGGAGATG CTGCACGCGC TGCTGGACCT GCCCTGCTTG 
ACGGCGGTGC TGGACCTGCA GCTCAGGTCA GCACCGGCTG CATCCGAGAG GCCACTCTGG 
GACACCTCTC TCAGGGCCCC CAGCTGCCTG GAGGCCTTCC GGGACCCGCA GTTCCAGGGT 
CTTTTCCAAT ACCTGCTGCG CCCCAAGGCC AGTGGCGCCA CTGAGAGGTT GGCGCCACTC 
CACCAGCTGC TGCAGCCCAT GGCCGGCTGT GCCCGCGTGG CCCAGTGTGC CCAGGCCGTG 
CCCACGCTGC TGCAGGCATT CTTCTCAGCA GTGACCCAGG TGGCTGACGG GTCCCTGATC 
AACCAGCTGG CGCTGCTGCT CCTGGGCAGG AGCGACTCGC TCTACCCGGC CCCAGGGTAC 
GCTGCCGGTG TGCACAGTGT GCTGAGTTCT CAGTTCCTGG CCCTGTGTAC GCTGAAACCC 
TCCCTGGTGG TGGAGCTGGC AAGAGACCTG CTGGAGTTCC TGGGCAGCGT GAATGGTCTC 
TGCAGCAGGG CGAGCCTCGT CACCAGCGTG GTGTGGGCCA TCGGCGAGTA CCTGTCGGTG 
ACCTACGATC GGAGGTGCAC CGTGGAGCAG ATCAACAAGT TCTTCGAAGC CCTGGAGGCT 
CTGCTATTCG AGGTCACCCA GTGCCGCCCC TCTGCTGCCC TGCCCAGGTG TCCCCCCCAG 
GTGGTCACCG TGCTGATGAC CACGCTGACG AAGCTGGCCT CCCGGAGCCA AGATCTGATC 
CCCAGGGCCT CTTTATTGCT GTCAAAGATG AGGACCCTGG CTCACAGTCC AGCCACCAGC 
TCCACGCACA GCGAGGAGGG CGCGGAAGCC ATCCGTACCC GGGCCACAGA GCTGCTGACC 
CTGCTGAAGA TGCCTAGCGT GGCCCAGTTT GTGCTCACAC CCAGCACGGA GGTGTGCAGC 
CCCCGCTATC ACCGCGATGC CAACACGGCC CTGCCCCTGG CCCTGCGCAC GGTCAGCCGG 
CTGGTGGAGA GGGAGGCCGG CCTCATGCCA GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The fifth adaptor protein complex.
PLoS biology9(10)e1001170(2011 Oct)
Hirst J,Barlow LD,Francisco GC,Sahlender DA,Seaman MN,Dacks JB,Robinson MS

A genome-scale DNA repair RNAi screen identifies SPG48 as a novel gene associated with hereditary spastic paraplegia.
PLoS biology8(6)e1000408(2010 Jun)
S?abicki M,Theis M,Krastev DB,Samsonov S,Mundwiller E,Junqueira M,Paszkowski-Rogacz M,Teyra J,Heninger AK,Poser I,Prieur F,Truchetto J,Confavreux C,Marelli C,Durr A,Camdessanche JP,Brice A,Shevchenko A,Pisabarro MT,Stevanin G,Buchholz F

Expressed sequence tag analysis of adult human iris for the NEIBank Project: steroid-response factors and similarities with retinal pigment epithelium.
Molecular vision8185-95(2002 Jun)
Wistow G,Bernstein SL,Ray S,Wyatt MK,Behal A,Touchman JW,Bouffard G,Smith D,Peterson K

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AP5Z1 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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