SPATA2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPATA2
Entrez Gene ID	9825
Full Name	spermatogenesis associated 2
Synonyms	PD1,PPP1R145,tamo
General protein information	Preferred Names spermatogenesis associated 2 Names spermatogenesis-associated protein 2 protein phosphatase 1, regulatory subunit 145 spermatogenesis associated PD1 spermatogenesis-associated protein PD1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.13
Disorder MIM:	607662

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135773.1	NP_001129245.1	spermatogenesis-associated protein 2
XM_011529116.1	XP_011527418.1	spermatogenesis-associated protein 2 isoform X1
XM_006723894.1	XP_006723957.1	spermatogenesis-associated protein 2 isoform X1
NM_006038.3	NP_006029.1	spermatogenesis-associated protein 2
NM_006038.4	NP_006029.1	spermatogenesis-associated protein 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SPATA2	NP_006029.1
Rattus norvegicus (Norway rat)	Spata2	NP_446127.3
Danio rerio (zebrafish)	spata2	NP_001073660.2
Xenopus tropicalis (tropical clawed frog)	spata2	XP_002933200.1
Pan troglodytes (chimpanzee)	SPATA2	XP_001167299.1
Mus musculus (house mouse)	Spata2	NP_739562.1
Gallus gallus (chicken)	SPATA2	NP_001007874.1
	SPATA2	XP_001098177.1
Canis lupus familiaris (dog)	SPATA2	XP_543047.2
Bos taurus (cattle)	SPATA2	NP_001179609.1

Gene Ontology
Bibliography

Related articles in PubMed

SPATA2 promotes CYLD activity and regulates TNF-induced NF-?B signaling and cell death.
Schlicher L, Wissler M, Preiss F, Brauns-Schubert P, Jakob C, Dumit V, Borner C, Dengjel J, Maurer U
EMBO reports17(10)1485-1497(2016 10)

SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
Elliott PR, Leske D, Hrdinka M, Bagola K, Fiil BK, McLaughlin SH, Wagstaff J, Volkmar N, Christianson JC, Kessler BM, Freund SM, Komander D, Gyrd-Hansen M
Molecular cell63(6)990-1005(2016 Sep)

SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes.
Wagner SA, Satpathy S, Beli P, Choudhary C
The EMBO journal35(17)1868-84(2016 09)

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
Kupka S, De Miguel D, Draber P, Martino L, Surinova S, Rittinger K, Walczak H
Cell reports16(9)2271-80(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study identifies SPATA2 as an important factor in the TNF signaling pathway with a substantial role for the effects mediated by the cytokine.
Title: SPATA2 promotes CYLD activity and regulates TNF-induced NF-?B signaling and cell death.

The data reveal SPATA2 as a high-affinity binding partner of CYLD and HOIP, and a regulatory component of linear ubiquitin chain assembly complex-mediated NF-kappaB signaling.
Title: SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.

The predicted PUB domain in the N-terminus of SPATA2 interacts with the USP domain of CYLD and SPATA2 is required for recruitment of CYLD to the TNF-alpha receptor-associated signaling complexes.
Title: SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes.

SPATA2 has been described as a previously unrecognized factor in the linear ubiquitin chain assembly complex-dependent signaling pathways that serves as an adaptor between HOIP and CYLD, thereby enabling recruitment of CYLD to signaling complexes.
Title: SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.

The aim of this study was to investigate whether the HCP5, TNIP1, TNFAIP3, SPATA2 and COG6 genes were genetic risk factors for psoriasis in Chinese population.
Title: Investigating the genetic association of HCP5, SPATA2, TNIP1, TNFAIP3 and COG6 with psoriasis in Chinese population.

The following SPATA2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPATA2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28204	NM_001135773.1 Latest version!	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu28204	NM_006038.4 Latest version!	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu28204	XM_006723894.1 Latest version!	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu28204	XM_011529116.1 Latest version!	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
View Old Accession Versions >>
OHu28204	NM_006038.3	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPATA2 ( NM_001135773.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001129245.1 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28204
Clone ID Related Accession (Same CDS sequence)	NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version	NM_001135773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	spermatogenesis-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK289644.1, BC009481.2, AL031685.18 and N62318.1. Transcript Variant: This variant (2) has an alternate 5' UTR exon, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289644.1, SRR1803616.9152.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END##

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001129245.1
CDS	351..1913
Translation	MGKPSSMDTKFKDDLFRKYVQFHESKVDTTTSRQRPGSDECLRVAASTLLSLHKVDPFYRFRLIQFYEVVESSLRSLSSSSLRALHGAFSMLETVGINLFLYPWKKEFRSIKTYTGPFVYYVKSTLLEEDIRAILSCMGYTPELGTAYKLRELVETLQVKMVSFELFLAKVECEQMLEIHSQVKDKGYSELDIVSERKSSAEDVRGCSDALRRRAEGREHLTASMSRVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPLKASLSLRKEPVATDVGDDLKDEIIRPSPSLLTMASSPHGSPDVLPPASPSNGPALLRGTYFSTQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

Target ORF information:

RefSeq Version	NM_001135773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135773.1

ORF Insert Sequence:

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPATA2 ( NM_006038.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006029.1 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28204
Clone ID Related Accession (Same CDS sequence)	NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version	NM_006038.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-19
Organism	Homo sapiens(human)
Product	spermatogenesis-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295536.1, BC009481.2, AL031685.18 and N62318.1. On Oct 11, 2008 this sequence version replaced NM_006038.2. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018300.1, SRR1660807.54080.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006029.1
CDS	267..1829
Translation	MGKPSSMDTKFKDDLFRKYVQFHESKVDTTTSRQRPGSDECLRVAASTLLSLHKVDPFYRFRLIQFYEVVESSLRSLSSSSLRALHGAFSMLETVGINLFLYPWKKEFRSIKTYTGPFVYYVKSTLLEEDIRAILSCMGYTPELGTAYKLRELVETLQVKMVSFELFLAKVECEQMLEIHSQVKDKGYSELDIVSERKSSAEDVRGCSDALRRRAEGREHLTASMSRVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPLKASLSLRKEPVATDVGDDLKDEIIRPSPSLLTMASSPHGSPDVLPPASPSNGPALLRGTYFSTQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

Target ORF information:

RefSeq Version	NM_006038.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006038.3

ORF Insert Sequence:

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPATA2 ( NM_006038.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_006029.1 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28204
Clone ID Related Accession (Same CDS sequence)	NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version	NM_006038.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	spermatogenesis-associated protein 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK295536.1, BC009481.2, AL031685.18 and N62318.1. On Nov 23, 2018 this sequence version replaced NM_006038.3. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.445800.1, AB018300.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000289431.10/ ENSP00000289431.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006029.1
CDS	253..1815
Translation	MGKPSSMDTKFKDDLFRKYVQFHESKVDTTTSRQRPGSDECLRVAASTLLSLHKVDPFYRFRLIQFYEVVESSLRSLSSSSLRALHGAFSMLETVGINLFLYPWKKEFRSIKTYTGPFVYYVKSTLLEEDIRAILSCMGYTPELGTAYKLRELVETLQVKMVSFELFLAKVECEQMLEIHSQVKDKGYSELDIVSERKSSAEDVRGCSDALRRRAEGREHLTASMSRVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPLKASLSLRKEPVATDVGDDLKDEIIRPSPSLLTMASSPHGSPDVLPPASPSNGPALLRGTYFSTQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

Target ORF information:

RefSeq Version	NM_006038.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006038.4

ORF Insert Sequence:

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPATA2 ( XM_011529116.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527418.1 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X2, mRNA.

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CloneID	OHu28204
Clone ID Related Accession (Same CDS sequence)	NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version	XM_011529116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spermatogenesis-associated protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527418.1
CDS	224..1786
Translation	MGKPSSMDTKFKDDLFRKYVQFHESKVDTTTSRQRPGSDECLRVAASTLLSLHKVDPFYRFRLIQFYEVVESSLRSLSSSSLRALHGAFSMLETVGINLFLYPWKKEFRSIKTYTGPFVYYVKSTLLEEDIRAILSCMGYTPELGTAYKLRELVETLQVKMVSFELFLAKVECEQMLEIHSQVKDKGYSELDIVSERKSSAEDVRGCSDALRRRAEGREHLTASMSRVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPLKASLSLRKEPVATDVGDDLKDEIIRPSPSLLTMASSPHGSPDVLPPASPSNGPALLRGTYFSTQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

Target ORF information:

RefSeq Version	XM_011529116.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529116.1

ORF Insert Sequence:

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPATA2 ( XM_006723894.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723957.1 Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu28204
Clone ID Related Accession (Same CDS sequence)	NM_001135773.1 , XM_011529116.1 , XM_006723894.1 , NM_006038.3 , NM_006038.4
Accession Version	XM_006723894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spermatogenesis-associated protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723957.1
CDS	187..1749
Translation	MGKPSSMDTKFKDDLFRKYVQFHESKVDTTTSRQRPGSDECLRVAASTLLSLHKVDPFYRFRLIQFYEVVESSLRSLSSSSLRALHGAFSMLETVGINLFLYPWKKEFRSIKTYTGPFVYYVKSTLLEEDIRAILSCMGYTPELGTAYKLRELVETLQVKMVSFELFLAKVECEQMLEIHSQVKDKGYSELDIVSERKSSAEDVRGCSDALRRRAEGREHLTASMSRVALQKSASERAAKDYYKPRVTKPSRSVDAYDSYWESRKPPLKASLSLRKEPVATDVGDDLKDEIIRPSPSLLTMASSPHGSPDVLPPASPSNGPALLRGTYFSTQDDVDLYTDSEPRATYRRQDALRPDVWLLRNDAHSLYHKRSPPAKESALSKCQSCGLSCSSSLCQRCDSLLTCPPASKPSAFPSKASTHDSLAHGASLREKYPGQTQGLDRLPHLHSKSKPSTTPTSRCGFCNRPGATNTCTQCSKVSCDACLSAYHYDPCYKKSELHKFMPNNQLNYKSTQLSHLVYR

Target ORF information:

RefSeq Version	XM_006723894.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spermatogenesis associated 2 (SPATA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723894.1

ORF Insert Sequence:

ATGGGGAAGC CCAGTTCAAT GGATACTAAA TTCAAGGATG ACTTATTTCG GAAGTACGTG 
CAGTTCCATG AGAGCAAAGT GGATACCACC ACCAGCAGGC AGCGGCCTGG CAGCGATGAG 
TGCCTGCGGG TGGCAGCCTC AACCCTGCTC AGCCTGCACA AGGTGGATCC CTTTTATCGA 
TTCCGGCTGA TCCAGTTCTA TGAGGTGGTG GAGAGCTCCT TGCGCTCGCT CAGCTCCTCT 
AGCCTGCGGG CTCTGCACGG CGCCTTCAGC ATGCTGGAGA CGGTGGGCAT CAACCTCTTC 
CTCTACCCGT GGAAGAAGGA ATTCAGAAGC ATCAAGACCT ACACGGGCCC TTTTGTTTAT 
TATGTCAAGT CGACATTACT GGAAGAGGAC ATCCGAGCCA TCCTGAGCTG CATGGGCTAC 
ACACCTGAGC TGGGCACTGC ATACAAGCTC AGAGAGCTCG TGGAGACCCT CCAGGTGAAG 
ATGGTCTCCT TTGAGCTCTT TCTGGCCAAA GTCGAGTGTG AGCAGATGCT AGAAATCCAC 
TCACAAGTGA AGGACAAGGG CTACTCCGAG CTGGACATTG TGAGCGAGCG CAAGAGCAGT 
GCAGAGGATG TGCGCGGCTG CTCGGACGCC CTGCGGCGGC GGGCAGAGGG CCGGGAGCAC 
CTGACGGCCT CCATGTCACG AGTGGCACTC CAGAAGTCGG CCAGCGAGCG GGCGGCCAAG 
GACTACTACA AGCCCCGCGT GACCAAGCCC TCGAGGTCAG TGGATGCCTA TGACAGCTAC 
TGGGAGAGCC GGAAGCCACC CCTGAAGGCC TCATTGAGTC TTCGGAAGGA GCCTGTGGCA 
ACGGATGTGG GGGACGACCT CAAGGATGAG ATCATCCGCC CATCCCCTTC GCTGCTGACC 
ATGGCCAGCT CCCCCCACGG CAGCCCGGAT GTGCTTCCAC CCGCCTCCCC CAGCAACGGC 
CCGGCCCTGC TGCGCGGTAC CTACTTCTCC ACTCAGGATG ACGTGGATCT GTACACAGAC 
TCTGAACCCA GGGCCACCTA CCGTCGGCAG GATGCTCTGC GGCCGGATGT GTGGCTGCTC 
AGAAACGATG CCCACTCCCT CTACCACAAG CGCTCGCCCC CTGCCAAAGA GTCCGCCCTC 
TCCAAGTGCC AAAGCTGCGG GCTGTCCTGC AGCTCCTCCC TCTGCCAGCG CTGTGACAGC 
CTGCTCACCT GTCCTCCAGC TTCCAAGCCC AGCGCCTTCC CCAGCAAGGC CTCGACTCAT 
GACAGCCTGG CCCACGGGGC ATCTCTGCGG GAGAAGTACC CAGGCCAGAC TCAGGGCCTC 
GACCGCCTCC CGCACCTTCA CTCCAAATCC AAGCCCTCCA CCACGCCCAC TTCCCGCTGT 
GGCTTCTGCA ACCGCCCAGG CGCCACCAAC ACCTGCACCC AGTGTTCAAA AGTCTCATGT 
GACGCCTGCC TCAGCGCTTA CCATTATGAC CCCTGCTACA AAAAGAGTGA GCTGCACAAG 
TTCATGCCCA ACAACCAGCT GAACTACAAG TCCACCCAGC TCTCCCATCT CGTGTACAGA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SPATA2 promotes CYLD activity and regulates TNF-induced NF-?B signaling and cell death.
EMBO reports17(10)1485-1497(2016 10)
Schlicher L,Wissler M,Preiss F,Brauns-Schubert P,Jakob C,Dumit V,Borner C,Dengjel J,Maurer U

SPATA2 Links CYLD to LUBAC, Activates CYLD, and Controls LUBAC Signaling.
Molecular cell63(6)990-1005(2016 Sep)
Elliott PR,Leske D,Hrdinka M,Bagola K,Fiil BK,McLaughlin SH,Wagstaff J,Volkmar N,Christianson JC,Kessler BM,Freund SM,Komander D,Gyrd-Hansen M

SPATA2 links CYLD to the TNF-? receptor signaling complex and modulates the receptor signaling outcomes.
The EMBO journal35(17)1868-84(2016 09)
Wagner SA,Satpathy S,Beli P,Choudhary C

SPATA2-Mediated Binding of CYLD to HOIP Enables CYLD Recruitment to Signaling Complexes.
Cell reports16(9)2271-80(2016 08)
Kupka S,De Miguel D,Draber P,Martino L,Surinova S,Rittinger K,Walczak H

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SPATA2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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