CKAP5 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CKAP5
Entrez Gene ID	9793
Full Name	cytoskeleton associated protein 5
Synonyms	CHTOG,MSPS,TOG,TOGp,ch-TOG
General protein information	Preferred Names cytoskeleton associated protein 5 Names cytoskeleton-associated protein 5 colonic and hepatic tumor over-expressed gene protein colonic and hepatic tumor overexpressed gene protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p11.2
Summary	This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011].
Disorder MIM:	611142

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008938.3	NP_001008938.1	cytoskeleton-associated protein 5 isoform a
NM_014756.3	NP_055571.2	cytoskeleton-associated protein 5 isoform b
NM_001008938.4	NP_001008938.1	cytoskeleton-associated protein 5 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CKAP5	XP_005631330.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011162	XP_309487.4
Rattus norvegicus (Norway rat)	Ckap5	XP_006234666.1
Xenopus tropicalis (tropical clawed frog)	ckap5	NP_001120559.1
Homo sapiens (human)	CKAP5	NP_001008938.1
Pan troglodytes (chimpanzee)	CKAP5	XP_001165890.1
Oryza sativa (rice)	Os01g0816400	NP_001044618.1
Arabidopsis thaliana (thale cress)	MOR1	NP_565811.2
	CKAP5	XP_002808118.1
Bos taurus (cattle)	CKAP5	NP_001178292.1
Mus musculus (house mouse)	Ckap5	NP_001159461.1
Gallus gallus (chicken)	CKAP5	NP_001186575.1
Danio rerio (zebrafish)	ckap5	NP_001032756.2
Drosophila melanogaster (fruit fly)	msps	NP_001163627.1

Gene Ontology
Bibliography

Related articles in PubMed

Eribulin targets a ch-TOG-dependent directed migration of cancer cells.
Chanez B, Gon?alves A, Badache A, Verdier-Pinard P
Oncotarget6(39)41667-78(2015 Dec)

Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module.
Roostalu J, Cade NI, Surrey T
Nature cell biology17(11)1422-34(2015 Nov)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

TTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation.
Watanabe T, Kakeno M, Matsui T, Sugiyama I, Arimura N, Matsuzawa K, Shirahige A, Ishidate F, Nishioka T, Taya S, Hoshino M, Kaibuchi K
The Journal of cell biology210(5)737-51(2015 Aug)

TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
Guti?rrez-Caballero C, Burgess SG, Bayliss R, Royle SJ
Biology open4(2)170-9(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Eribulin binding to the tip of microtubules and subsequent loss of ch-TOG is a priming event leading to alterations in microtubule dynamics and breast cancer cell migration.
Title: Eribulin targets a ch-TOG-dependent directed migration of cancer cells.

Data show that cytoskeleton associated protein 5 (chTOG) only weakly promotes importin-regulated microtubule nucleation, but acts synergistically with microtubule- associated protein TPX2.
Title: Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module.

Aurora-A kinase does not regulate TACC3-chTOG complex formation, indicating that Aurora-A solely functions as a recruitment factor for the TACC3-chTOG complex to centrosomes and proximal mitotic spindles.
Title: The centrosomal adaptor TACC3 and the microtubule polymerase chTOG interact via defined C-terminal subdomains in an Aurora-A kinase-independent manner.

Data show that five genes CKAP5, KPNB1, RAN, TPX2 and KIF11 were shown to be essential for tumor cell survival in both head and neck squamous cell carcinoma (HNSCC)and non-small cell lung cancer (NSCLC), but most particularly in HNSCC.
Title: Functional genetic screens identify genes essential for tumor cell survival in head and neck and lung cancer.

These observations indicate that EB1 and ch-TOG regulate microtubule organisation differently via distinct regions in the plus ends of microtubules.
Title: Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells.

The following CKAP5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CKAP5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14016	NM_014756.3 Latest version!	Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu19569	NM_001008938.4 Latest version!	Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
View Old Accession Versions >>
OHu19569	NM_001008938.3	Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CKAP5 ( NM_014756.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055571.2 Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14016
Clone ID Related Accession (Same CDS sequence)	NM_014756.3
Accession Version	NM_014756.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	cytoskeleton-associated protein 5 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP282525.1, BC120869.1, X92474.1, D43948.1, CB052595.1 and BM980015.1. On Aug 27, 2011 this sequence version replaced NM_014756.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 3' coding region, compared to variant 1. The resulting isoform (b) lacks an internal segment, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.223511.1, SRR1803611.76723.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGATG ACAGTGAGTG GTTGAAACTG CCAGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GGTTAAGTGG GTATGAAGAG GCCCTGAAGA TCTTCCAGAA AATAAAGGAT 
GAAAAGAGCC CAGAGTGGTC CAAATTTTTA GGATTGATCA AAAAATTTGT CACTGATTCC 
AATGCAGTGG TTCAATTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACAGG AGAAGTTGTG TCAGGTGTTG TAAGTAAGGT GTTCAATCAA 
CCTAAAGCTA AAGCCAAGGA GCTGGGCATA GAGATCTGTC TTATGTACAT AGAGATTGAG 
AAAGGAGAGG CTGTTCAAGA AGAGCTCCTG AAAGGCTTGG ACAATAAGAA TCCCAAGATC 
ATAGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTTGGTTC CAAAATCATC 
TTGCTTAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AGTCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAACTAAT TGCTGTGGAG ATTTACAGAT GGATTCGGGA TGCTCTGAGA 
CCCCCATTAC AAAATATAAA CTCTGTTCAG TTGAAAGAAC TAGAAGAAGA ATGGGTCAAA 
CTGCCAACAA GTGCTCCTAG ACCTACTCGA TTTCTTCGTT CCCAACAAGA ACTAGAAGCT 
AAATTGGAAC AACAACAGTC TGCTGGTGGA GATGCTGAAG GAGGTGGTGA TGATGGTGAT 
GAGGTGCCAC AAATAGATGC TTATGAGCTT TTAGAAGCTG TAGAAATCCT TTCCAAACTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAGAGAAA AGAGGCCCTG 
GAGTCTGTAG AAGTACTAAT AAAAAACCCC AAACTGGAAG CTGGCGATTA TGCAGATTTA 
GTAAAAGCAT TAAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT GGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGGAAGA AATTTGGACA ATATGCAGGA 
CATGTTGTGC CAACCATCTT GGAGAAATTC AAAGAGAAGA AACCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AGCAGACATC TCTTTTTATT 
GCAAGAAGTT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTGCT AAAGCCCTTT 
TGTGCTGCAC TACTTAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCCGCATTT 
GAAGCATTGG GTACTGCTTT GAAGGTGGTT GGCGAGAAAG CAGTAAACCC ATTCCTAGCT 
GATGTGGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAGGT AGAACTGATA 
CATGGTAAGA AAGCTGGACT AGCTGCTGAT AAGAAGGAAT TCAAACCTCT GCCTGGAAGG 
ACTGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACATTTCTGC ACCCAAACCA 
GGACCTCTAA AAAAGGCACC TGCTGCTAAG GCTGGTGGGC CACCAAAAAA GGGGAAACCA 
GCTGCACCAG GAGGCGCAGG GAATACTGGA ACCAAGAACA AGAAAGGACT GGAGACTAAA 
GAAATAGTGG AGCCTGAGCT CTCGATAGAA GTATGTGAAG AAAAAGCTTC AGCTGTTCTT 
CCCCCTACCT GTATACAGCT TCTTGACAGC AGTAACTGGA AAGAAAGGCT GGCTTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAGCTAATG GACCGAACTG AAATGCCATG CCAGGCATTA 
GTGAGGATGC TAGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCATA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC GTCAGCTCAG 
GTTGTATTAG ATGGCCTTGT GGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCAAAA 
GAAGCTATGA CAGCAATAGC CGAAGCCTGT ATGTTACCAT GGACTGCTGA ACAGGTTGTG 
TCAATGGCTT TCTCACAAAA GAATCCCAAA AATCAGTCAG AAACTCTGAA TTGGCTATCA 
AATGCCATAA AAGAATTTGG TTTTTCTGGG TTGAATGTCA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACTG CTGCCATAAC CCTGCTTGGC 
GTGATGTATC TGTATGTTGG TCCCTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAAAGTCC ACCTGCTCCA 
ACCAGAGGAA TTTCCAAGCA TAGCACAAGT GGTACAGATG AAGGAGAAGA TGGAGATGAA 
CCAGATGACG GGAGCAATGA TGTCGTTGAT CTTTTGCCGA GGACGGAGAT CAGTGATAAA 
ATCACTTCAG AGTTGGTATC TAAGATTGGT GATAAGAATT GGAAGATTAG GAAAGAAGGC 
CTAGATGAAG TGGCAGGTAT TATTAATGAC GCAAAATTTA TCCAACCGAA TATAGGTGAA 
CTTCCAACTG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT ACAGCAAACG 
CTGAATATCC TGCAACAACT GGCAGTAGCC ATGGGCCCAA ATATTAAGCA ACATGTAAAA 
AATTTAGGCA TCCCTATCAT CACAGTCCTT GGAGACAGCA AGAACAATGT TCGAGCTGCT 
GCCCTAGCGA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAAGGA 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC TACTCTTCGT TCCACCCCTA CAGACCTTAT CCTTTGTGTT 
CCTCATCTCT ACTCCTGCCT AGAAGATCGA AATGGAGATG TGCGAAAGAA GGCCCAAGAT 
GCCTTGCCAT TCTTCATGAT GCATTTAGGA TATGAAAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAGCCAA CTTCTAAAGA TCAGGTATTG GCCATGCTAG AGAAAGCCAA AGTTAACATG 
CCAGCCAAGC CTGCTCCACC CACTAAAGCA ACTTCTAAAC CAATGGGAGG GTCCGCTCCA 
GCCAAATTCC AGCCTGCATC AGCACCTGCT GAAGATTGTA TTTCCAGCAG TACAGAACCC 
AAACCTGATC CAAAAAAGGC CAAAGCTCCA GGATTATCCT CTAAAGCAAA GAGTGCACAA 
GGGAAGAAGA TGCCAAGCAA AACCAGCTTA AAGGAGGATG AAGACAAATC CGGGCCTATT 
TTTATTGTTG TTCCAAATGG AAAAGAGCAA AGGATGAAAG ATGAAAAAGG ATTGAAGGTG 
CTAAAGTGGA ATTTTACTAC CCCACGGGAT GAATACATTG AGCAACTAAA GACTCAAATG 
TCTAGCTGTG TGGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAAGCCC TTGCTGTTAT GGTTGATCAC TTGGAGAGTG AAAAAGAAGG AGTTATTGGT 
TGCCTGGATC TTATCTTAAA GTGGCTTACC CTGAGGTTTT TTGACACCAA TACAAGCGTC 
CTGATGAAAG CACTAGAATA TTTAAAATTG CTCTTCACCT TGCTAAGTGA AGAAGAATAT 
CATCTTACTG AGAATGAAGC ATCTTCCTTC ATCCCCTATC TTGTCGTCAA GGTTGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTTCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCTAGCA AGATGTTTCC CTTTATCATG GAAGGAACCA AATCCAAAAA CTCTAAGCAG 
AGAGCAGAGT GCCTGGAAGA GCTGGGATGT CTGGTTGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAA ATAGCTGTTC ACATAGGAGA CCGTGACAAT 
GCTGTACGCA ATGCTGCACT CAACACCATT GTAACGGTGT ACAATGTACA TGGGGATCAG 
GTGTTCAAAC TGATTGGAAA TCTTTCTGAA AAGGATATGA GCATGCTCGA GGAGAGGATT 
AAGCGGTCAG CAAAGAGACC CTCTGCTGCA CCAATAAAAC AGGTGGAAGA GAAACCTCAG 
CGTGCACAGA ACATAAGCTC CAATGCCAAC ATGTTACGCA AGGGACCAGC TGAGGACATG 
TCTTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTC 
CGCCGAGAAT TCCAGCTGGA TCTAGATGAG ATTGAGAATG ACAATGGTAC AGTCCGATGT 
GAAATGCCAG AACTTGTTCA GCACAAACTG GATGACATTT TTGAGCCAGT CCTTATTCCT 
GAACCCAAGA TCCGGGCTGT TTCTCCACAC TTCGATGACA TGCACAGTAA TACAGCATCC 
ACAATCAATT TCATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG TATCCAAGCT 
CTGACACAGC TGTTTCAGAT AGAGAGCCTT GCCCGGGAGG CCTCCACTGG AGTACTAAAA 
GACCTAATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GGATTGAAGA TCTTGAGGAA 
GGACAACAGG TCATCCGCTC TGTGAACCTC TTGGTGGTGA AGGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTACTTGTT TTGCTCCAAG ACAGCCTGCT AGCAACAGCC 
AGTTCTCCCA AATTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGAATGGT TCGACTGTTG 
CCTGATACCA TCAATAGCAT TAACCTAGAC AGAATTCTTC TGGATATCCA CATTTTCATG 
AAGGTCTTCC CCAAAGAGAA ACTGAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAGACCCTGC TACACACCTT ATGCAAATTA AAAGGGCCCA AGATCCTGGA CCACCTAACG 
ATGATCGACA ACAAAAACGA GTCTGAGCTG GAGGCCCATC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACTGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATA 
GATGAAAAAT CATCAAAGGC CAAAGTGAAT GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AAAACACTAA AGAGGGACTA GCAGAGTTAT ATGAATATAA GAAGAAATAC 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AATTCCTCAC AGTTCTTCCA GAGCTATGTC 
GAAAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGTCGTAT TTCCACTTCA 
ACAGGCATCT CCCCTCAGAT GGAAGTCACA TGTGTGCCCA CGCCCACAAG CACAGTGTCC 
TCCATAGGTA ACACAAATGG GGAAGAAGTG GGGCCATCTG TCTACTTGGA AAGGCTAAAG 
ATCCTCCGAC AGCGATGTGG TCTGGACAAC ACAAAGCAAG ATGACCGACC TCCTTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCTACT GTCGCCTCTT CCACAGACAT GCTCCACAGC 
AAACTCTCTC AGCTCCGGGA GTCACGGGAG CAGCACCAGC ATTCAGACCT GGATTCTAAC 
CAGACTCACT CTTCAGGAAC TGTGACCTCC TCCTCCTCCA CAGCTAACAT AGACGACTTG 
AAAAAAAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055571.2
CDS	127..6045
Translation	MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEWVKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELIHGKKAGLAADKKEFKPLPGRTAASGAAGDKDTKDISAPKPGPLKKAPAAKAGGPPKKGKPAAPGGAGNTGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQVVLDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVVSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTAAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDEPDDGSNDVVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGELPTALKGRLNDSNKILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKVNMPAKPAPPTKATSKPMGGSAPAKFQPASAPAEDCISSSTEPKPDPKKAKAPGLSSKAKSAQGKKMPSKTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSVLMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVEEKPQRAQNISSNANMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	NM_014756.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014756.3

ORF Insert Sequence:

ATGGGAGATG ACAGTGAGTG GTTGAAACTG CCAGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GGTTAAGTGG GTATGAAGAG GCCCTGAAGA TCTTCCAGAA AATAAAGGAT 
GAAAAGAGCC CAGAGTGGTC CAAATTTTTA GGATTGATCA AAAAATTTGT CACTGATTCC 
AATGCAGTGG TTCAATTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACAGG AGAAGTTGTG TCAGGTGTTG TAAGTAAGGT GTTCAATCAA 
CCTAAAGCTA AAGCCAAGGA GCTGGGCATA GAGATCTGTC TTATGTACAT AGAGATTGAG 
AAAGGAGAGG CTGTTCAAGA AGAGCTCCTG AAAGGCTTGG ACAATAAGAA TCCCAAGATC 
ATAGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTTGGTTC CAAAATCATC 
TTGCTTAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AGTCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAACTAAT TGCTGTGGAG ATTTACAGAT GGATTCGGGA TGCTCTGAGA 
CCCCCATTAC AAAATATAAA CTCTGTTCAG TTGAAAGAAC TAGAAGAAGA ATGGGTCAAA 
CTGCCAACAA GTGCTCCTAG ACCTACTCGA TTTCTTCGTT CCCAACAAGA ACTAGAAGCT 
AAATTGGAAC AACAACAGTC TGCTGGTGGA GATGCTGAAG GAGGTGGTGA TGATGGTGAT 
GAGGTGCCAC AAATAGATGC TTATGAGCTT TTAGAAGCTG TAGAAATCCT TTCCAAACTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAGAGAAA AGAGGCCCTG 
GAGTCTGTAG AAGTACTAAT AAAAAACCCC AAACTGGAAG CTGGCGATTA TGCAGATTTA 
GTAAAAGCAT TAAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT GGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGGAAGA AATTTGGACA ATATGCAGGA 
CATGTTGTGC CAACCATCTT GGAGAAATTC AAAGAGAAGA AACCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AGCAGACATC TCTTTTTATT 
GCAAGAAGTT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTGCT AAAGCCCTTT 
TGTGCTGCAC TACTTAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCCGCATTT 
GAAGCATTGG GTACTGCTTT GAAGGTGGTT GGCGAGAAAG CAGTAAACCC ATTCCTAGCT 
GATGTGGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAGGT AGAACTGATA 
CATGGTAAGA AAGCTGGACT AGCTGCTGAT AAGAAGGAAT TCAAACCTCT GCCTGGAAGG 
ACTGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACATTTCTGC ACCCAAACCA 
GGACCTCTAA AAAAGGCACC TGCTGCTAAG GCTGGTGGGC CACCAAAAAA GGGGAAACCA 
GCTGCACCAG GAGGCGCAGG GAATACTGGA ACCAAGAACA AGAAAGGACT GGAGACTAAA 
GAAATAGTGG AGCCTGAGCT CTCGATAGAA GTATGTGAAG AAAAAGCTTC AGCTGTTCTT 
CCCCCTACCT GTATACAGCT TCTTGACAGC AGTAACTGGA AAGAAAGGCT GGCTTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAGCTAATG GACCGAACTG AAATGCCATG CCAGGCATTA 
GTGAGGATGC TAGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCATA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC GTCAGCTCAG 
GTTGTATTAG ATGGCCTTGT GGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCAAAA 
GAAGCTATGA CAGCAATAGC CGAAGCCTGT ATGTTACCAT GGACTGCTGA ACAGGTTGTG 
TCAATGGCTT TCTCACAAAA GAATCCCAAA AATCAGTCAG AAACTCTGAA TTGGCTATCA 
AATGCCATAA AAGAATTTGG TTTTTCTGGG TTGAATGTCA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACTG CTGCCATAAC CCTGCTTGGC 
GTGATGTATC TGTATGTTGG TCCCTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAAAGTCC ACCTGCTCCA 
ACCAGAGGAA TTTCCAAGCA TAGCACAAGT GGTACAGATG AAGGAGAAGA TGGAGATGAA 
CCAGATGACG GGAGCAATGA TGTCGTTGAT CTTTTGCCGA GGACGGAGAT CAGTGATAAA 
ATCACTTCAG AGTTGGTATC TAAGATTGGT GATAAGAATT GGAAGATTAG GAAAGAAGGC 
CTAGATGAAG TGGCAGGTAT TATTAATGAC GCAAAATTTA TCCAACCGAA TATAGGTGAA 
CTTCCAACTG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT ACAGCAAACG 
CTGAATATCC TGCAACAACT GGCAGTAGCC ATGGGCCCAA ATATTAAGCA ACATGTAAAA 
AATTTAGGCA TCCCTATCAT CACAGTCCTT GGAGACAGCA AGAACAATGT TCGAGCTGCT 
GCCCTAGCGA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAAGGA 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC TACTCTTCGT TCCACCCCTA CAGACCTTAT CCTTTGTGTT 
CCTCATCTCT ACTCCTGCCT AGAAGATCGA AATGGAGATG TGCGAAAGAA GGCCCAAGAT 
GCCTTGCCAT TCTTCATGAT GCATTTAGGA TATGAAAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAGCCAA CTTCTAAAGA TCAGGTATTG GCCATGCTAG AGAAAGCCAA AGTTAACATG 
CCAGCCAAGC CTGCTCCACC CACTAAAGCA ACTTCTAAAC CAATGGGAGG GTCCGCTCCA 
GCCAAATTCC AGCCTGCATC AGCACCTGCT GAAGATTGTA TTTCCAGCAG TACAGAACCC 
AAACCTGATC CAAAAAAGGC CAAAGCTCCA GGATTATCCT CTAAAGCAAA GAGTGCACAA 
GGGAAGAAGA TGCCAAGCAA AACCAGCTTA AAGGAGGATG AAGACAAATC CGGGCCTATT 
TTTATTGTTG TTCCAAATGG AAAAGAGCAA AGGATGAAAG ATGAAAAAGG ATTGAAGGTG 
CTAAAGTGGA ATTTTACTAC CCCACGGGAT GAATACATTG AGCAACTAAA GACTCAAATG 
TCTAGCTGTG TGGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAAGCCC TTGCTGTTAT GGTTGATCAC TTGGAGAGTG AAAAAGAAGG AGTTATTGGT 
TGCCTGGATC TTATCTTAAA GTGGCTTACC CTGAGGTTTT TTGACACCAA TACAAGCGTC 
CTGATGAAAG CACTAGAATA TTTAAAATTG CTCTTCACCT TGCTAAGTGA AGAAGAATAT 
CATCTTACTG AGAATGAAGC ATCTTCCTTC ATCCCCTATC TTGTCGTCAA GGTTGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTTCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCTAGCA AGATGTTTCC CTTTATCATG GAAGGAACCA AATCCAAAAA CTCTAAGCAG 
AGAGCAGAGT GCCTGGAAGA GCTGGGATGT CTGGTTGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAA ATAGCTGTTC ACATAGGAGA CCGTGACAAT 
GCTGTACGCA ATGCTGCACT CAACACCATT GTAACGGTGT ACAATGTACA TGGGGATCAG 
GTGTTCAAAC TGATTGGAAA TCTTTCTGAA AAGGATATGA GCATGCTCGA GGAGAGGATT 
AAGCGGTCAG CAAAGAGACC CTCTGCTGCA CCAATAAAAC AGGTGGAAGA GAAACCTCAG 
CGTGCACAGA ACATAAGCTC CAATGCCAAC ATGTTACGCA AGGGACCAGC TGAGGACATG 
TCTTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTC 
CGCCGAGAAT TCCAGCTGGA TCTAGATGAG ATTGAGAATG ACAATGGTAC AGTCCGATGT 
GAAATGCCAG AACTTGTTCA GCACAAACTG GATGACATTT TTGAGCCAGT CCTTATTCCT 
GAACCCAAGA TCCGGGCTGT TTCTCCACAC TTCGATGACA TGCACAGTAA TACAGCATCC 
ACAATCAATT TCATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG TATCCAAGCT 
CTGACACAGC TGTTTCAGAT AGAGAGCCTT GCCCGGGAGG CCTCCACTGG AGTACTAAAA 
GACCTAATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GGATTGAAGA TCTTGAGGAA 
GGACAACAGG TCATCCGCTC TGTGAACCTC TTGGTGGTGA AGGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTACTTGTT TTGCTCCAAG ACAGCCTGCT AGCAACAGCC 
AGTTCTCCCA AATTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGAATGGT TCGACTGTTG 
CCTGATACCA TCAATAGCAT TAACCTAGAC AGAATTCTTC TGGATATCCA CATTTTCATG 
AAGGTCTTCC CCAAAGAGAA ACTGAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAGACCCTGC TACACACCTT ATGCAAATTA AAAGGGCCCA AGATCCTGGA CCACCTAACG 
ATGATCGACA ACAAAAACGA GTCTGAGCTG GAGGCCCATC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACTGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATA 
GATGAAAAAT CATCAAAGGC CAAAGTGAAT GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AAAACACTAA AGAGGGACTA GCAGAGTTAT ATGAATATAA GAAGAAATAC 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AATTCCTCAC AGTTCTTCCA GAGCTATGTC 
GAAAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGTCGTAT TTCCACTTCA 
ACAGGCATCT CCCCTCAGAT GGAAGTCACA TGTGTGCCCA CGCCCACAAG CACAGTGTCC 
TCCATAGGTA ACACAAATGG GGAAGAAGTG GGGCCATCTG TCTACTTGGA AAGGCTAAAG 
ATCCTCCGAC AGCGATGTGG TCTGGACAAC ACAAAGCAAG ATGACCGACC TCCTTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCTACT GTCGCCTCTT CCACAGACAT GCTCCACAGC 
AAACTCTCTC AGCTCCGGGA GTCACGGGAG CAGCACCAGC ATTCAGACCT GGATTCTAAC 
CAGACTCACT CTTCAGGAAC TGTGACCTCC TCCTCCTCCA CAGCTAACAT AGACGACTTG 
AAAAAAAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( NM_001008938.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008938.1 Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 1, mRNA.

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CloneID	OHu19569
Clone ID Related Accession (Same CDS sequence)	NM_001008938.3 , NM_001008938.4
Accession Version	NM_001008938.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	cytoskeleton-associated protein 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP282525.1, BC120869.1, D43948.1, CB052595.1 and BM980015.1. This sequence is a reference standard in the RefSeqGene project. On Aug 30, 2011 this sequence version replaced NM_001008938.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.198539.1, SRR1803614.45127.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGAGATG ACAGTGAGTG GTTGAAACTG CCAGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GGTTAAGTGG GTATGAAGAG GCCCTGAAGA TCTTCCAGAA AATAAAGGAT 
GAAAAGAGCC CAGAGTGGTC CAAATTTTTA GGATTGATCA AAAAATTTGT CACTGATTCC 
AATGCAGTGG TTCAATTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACAGG AGAAGTTGTG TCAGGTGTTG TAAGTAAGGT GTTCAATCAA 
CCTAAAGCTA AAGCCAAGGA GCTGGGCATA GAGATCTGTC TTATGTACAT AGAGATTGAG 
AAAGGAGAGG CTGTTCAAGA AGAGCTCCTG AAAGGCTTGG ACAATAAGAA TCCCAAGATC 
ATAGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTTGGTTC CAAAATCATC 
TTGCTTAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AGTCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAACTAAT TGCTGTGGAG ATTTACAGAT GGATTCGGGA TGCTCTGAGA 
CCCCCATTAC AAAATATAAA CTCTGTTCAG TTGAAAGAAC TAGAAGAAGA ATGGGTCAAA 
CTGCCAACAA GTGCTCCTAG ACCTACTCGA TTTCTTCGTT CCCAACAAGA ACTAGAAGCT 
AAATTGGAAC AACAACAGTC TGCTGGTGGA GATGCTGAAG GAGGTGGTGA TGATGGTGAT 
GAGGTGCCAC AAATAGATGC TTATGAGCTT TTAGAAGCTG TAGAAATCCT TTCCAAACTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAGAGAAA AGAGGCCCTG 
GAGTCTGTAG AAGTACTAAT AAAAAACCCC AAACTGGAAG CTGGCGATTA TGCAGATTTA 
GTAAAAGCAT TAAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT GGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGGAAGA AATTTGGACA ATATGCAGGA 
CATGTTGTGC CAACCATCTT GGAGAAATTC AAAGAGAAGA AACCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AGCAGACATC TCTTTTTATT 
GCAAGAAGTT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTGCT AAAGCCCTTT 
TGTGCTGCAC TACTTAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCCGCATTT 
GAAGCATTGG GTACTGCTTT GAAGGTGGTT GGCGAGAAAG CAGTAAACCC ATTCCTAGCT 
GATGTGGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAGGT AGAACTGATA 
CATGGTAAGA AAGCTGGACT AGCTGCTGAT AAGAAGGAAT TCAAACCTCT GCCTGGAAGG 
ACTGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACATTTCTGC ACCCAAACCA 
GGACCTCTAA AAAAGGCACC TGCTGCTAAG GCTGGTGGGC CACCAAAAAA GGGGAAACCA 
GCTGCACCAG GAGGCGCAGG GAATACTGGA ACCAAGAACA AGAAAGGACT GGAGACTAAA 
GAAATAGTGG AGCCTGAGCT CTCGATAGAA GTATGTGAAG AAAAAGCTTC AGCTGTTCTT 
CCCCCTACCT GTATACAGCT TCTTGACAGC AGTAACTGGA AAGAAAGGCT GGCTTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAGCTAATG GACCGAACTG AAATGCCATG CCAGGCATTA 
GTGAGGATGC TAGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCATA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC GTCAGCTCAG 
GTTGTATTAG ATGGCCTTGT GGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCAAAA 
GAAGCTATGA CAGCAATAGC CGAAGCCTGT ATGTTACCAT GGACTGCTGA ACAGGTTGTG 
TCAATGGCTT TCTCACAAAA GAATCCCAAA AATCAGTCAG AAACTCTGAA TTGGCTATCA 
AATGCCATAA AAGAATTTGG TTTTTCTGGG TTGAATGTCA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACTG CTGCCATAAC CCTGCTTGGC 
GTGATGTATC TGTATGTTGG TCCCTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAAAGTCC ACCTGCTCCA 
ACCAGAGGAA TTTCCAAGCA TAGCACAAGT GGTACAGATG AAGGAGAAGA TGGAGATGAA 
CCAGATGACG GGAGCAATGA TGTCGTTGAT CTTTTGCCGA GGACGGAGAT CAGTGATAAA 
ATCACTTCAG AGTTGGTATC TAAGATTGGT GATAAGAATT GGAAGATTAG GAAAGAAGGC 
CTAGATGAAG TGGCAGGTAT TATTAATGAC GCAAAATTTA TCCAACCGAA TATAGGTGAA 
CTTCCAACTG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT ACAGCAAACG 
CTGAATATCC TGCAACAACT GGCAGTAGCC ATGGGCCCAA ATATTAAGCA ACATGTAAAA 
AATTTAGGCA TCCCTATCAT CACAGTCCTT GGAGACAGCA AGAACAATGT TCGAGCTGCT 
GCCCTAGCGA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAAGGA 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC TACTCTTCGT TCCACCCCTA CAGACCTTAT CCTTTGTGTT 
CCTCATCTCT ACTCCTGCCT AGAAGATCGA AATGGAGATG TGCGAAAGAA GGCCCAAGAT 
GCCTTGCCAT TCTTCATGAT GCATTTAGGA TATGAAAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAGCCAA CTTCTAAAGA TCAGGTATTG GCCATGCTAG AGAAAGCCAA AGTTAACATG 
CCAGCCAAGC CTGCTCCACC CACTAAAGCA ACTTCTAAAC CAATGGGAGG GTCCGCTCCA 
GCCAAATTCC AGCCTGCATC AGCACCTGCT GAAGATTGTA TTTCCAGCAG TACAGAACCC 
AAACCTGATC CAAAAAAGGC CAAAGCTCCA GGATTATCCT CTAAAGCAAA GAGTGCACAA 
GGGAAGAAGA TGCCAAGCAA AACCAGCTTA AAGGAGGATG AAGACAAATC CGGGCCTATT 
TTTATTGTTG TTCCAAATGG AAAAGAGCAA AGGATGAAAG ATGAAAAAGG ATTGAAGGTG 
CTAAAGTGGA ATTTTACTAC CCCACGGGAT GAATACATTG AGCAACTAAA GACTCAAATG 
TCTAGCTGTG TGGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAAGCCC TTGCTGTTAT GGTTGATCAC TTGGAGAGTG AAAAAGAAGG AGTTATTGGT 
TGCCTGGATC TTATCTTAAA GTGGCTTACC CTGAGGTTTT TTGACACCAA TACAAGCGTC 
CTGATGAAAG CACTAGAATA TTTAAAATTG CTCTTCACCT TGCTAAGTGA AGAAGAATAT 
CATCTTACTG AGAATGAAGC ATCTTCCTTC ATCCCCTATC TTGTCGTCAA GGTTGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTTCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCTAGCA AGATGTTTCC CTTTATCATG GAAGGAACCA AATCCAAAAA CTCTAAGCAG 
AGAGCAGAGT GCCTGGAAGA GCTGGGATGT CTGGTTGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAA ATAGCTGTTC ACATAGGAGA CCGTGACAAT 
GCTGTACGCA ATGCTGCACT CAACACCATT GTAACGGTGT ACAATGTACA TGGGGATCAG 
GTGTTCAAAC TGATTGGAAA TCTTTCTGAA AAGGATATGA GCATGCTCGA GGAGAGGATT 
AAGCGGTCAG CAAAGAGACC CTCTGCTGCA CCAATAAAAC AGGTGGAAGA GAAACCTCAG 
CGTGCACAGA ACATAAGCTC CAATGCCAAC ATGTTACGCA AGGGACCAGC TGAGGACATG 
TCTTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTC 
CGCCGAGAAT TCCAGCTGGA TCTAGATGAG ATTGAGAATG ACAATGGTAC AGTCCGATGT 
GAAATGCCAG AACTTGTTCA GCACAAACTG GATGACATTT TTGAGCCAGT CCTTATTCCT 
GAACCCAAGA TCCGGGCTGT TTCTCCACAC TTCGATGACA TGCACAGTAA TACAGCATCC 
ACAATCAATT TCATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG TATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCCGGCCAT 
ATTGATCAGT TTCTGATAGC CACTTTTATG CAGCTAAGAC TCATCTACAA CACACACATG 
GCAGATGAGA AATTGGAGAA GGACGAGATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAGAGCCTT GCCCGGGAGG CCTCCACTGG AGTACTAAAA 
GACCTAATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GGATTGAAGA TCTTGAGGAA 
GGACAACAGG TCATCCGCTC TGTGAACCTC TTGGTGGTGA AGGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTACTTGTT TTGCTCCAAG ACAGCCTGCT AGCAACAGCC 
AGTTCTCCCA AATTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGAATGGT TCGACTGTTG 
CCTGATACCA TCAATAGCAT TAACCTAGAC AGAATTCTTC TGGATATCCA CATTTTCATG 
AAGGTCTTCC CCAAAGAGAA ACTGAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAGACCCTGC TACACACCTT ATGCAAATTA AAAGGGCCCA AGATCCTGGA CCACCTAACG 
ATGATCGACA ACAAAAACGA GTCTGAGCTG GAGGCCCATC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACTGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATA 
GATGAAAAAT CATCAAAGGC CAAAGTGAAT GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AAAACACTAA AGAGGGACTA GCAGAGTTAT ATGAATATAA GAAGAAATAC 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AATTCCTCAC AGTTCTTCCA GAGCTATGTC 
GAAAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGTCGTAT TTCCACTTCA 
ACAGGCATCT CCCCTCAGAT GGAAGTCACA TGTGTGCCCA CGCCCACAAG CACAGTGTCC 
TCCATAGGTA ACACAAATGG GGAAGAAGTG GGGCCATCTG TCTACTTGGA AAGGCTAAAG 
ATCCTCCGAC AGCGATGTGG TCTGGACAAC ACAAAGCAAG ATGACCGACC TCCTTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCTACT GTCGCCTCTT CCACAGACAT GCTCCACAGC 
AAACTCTCTC AGCTCCGGGA GTCACGGGAG CAGCACCAGC ATTCAGACCT GGATTCTAAC 
CAGACTCACT CTTCAGGAAC TGTGACCTCC TCCTCCTCCA CAGCTAACAT AGACGACTTG 
AAAAAAAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008938.1
CDS	127..6225
Translation	MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEWVKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELIHGKKAGLAADKKEFKPLPGRTAASGAAGDKDTKDISAPKPGPLKKAPAAKAGGPPKKGKPAAPGGAGNTGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQVVLDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVVSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTAAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDEPDDGSNDVVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGELPTALKGRLNDSNKILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKVNMPAKPAPPTKATSKPMGGSAPAKFQPASAPAEDCISSSTEPKPDPKKAKAPGLSSKAKSAQGKKMPSKTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSVLMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVEEKPQRAQNISSNANMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	NM_001008938.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008938.3

ORF Insert Sequence:

ATGGGAGATG ACAGTGAGTG GTTGAAACTG CCAGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GGTTAAGTGG GTATGAAGAG GCCCTGAAGA TCTTCCAGAA AATAAAGGAT 
GAAAAGAGCC CAGAGTGGTC CAAATTTTTA GGATTGATCA AAAAATTTGT CACTGATTCC 
AATGCAGTGG TTCAATTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACAGG AGAAGTTGTG TCAGGTGTTG TAAGTAAGGT GTTCAATCAA 
CCTAAAGCTA AAGCCAAGGA GCTGGGCATA GAGATCTGTC TTATGTACAT AGAGATTGAG 
AAAGGAGAGG CTGTTCAAGA AGAGCTCCTG AAAGGCTTGG ACAATAAGAA TCCCAAGATC 
ATAGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTTGGTTC CAAAATCATC 
TTGCTTAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AGTCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAACTAAT TGCTGTGGAG ATTTACAGAT GGATTCGGGA TGCTCTGAGA 
CCCCCATTAC AAAATATAAA CTCTGTTCAG TTGAAAGAAC TAGAAGAAGA ATGGGTCAAA 
CTGCCAACAA GTGCTCCTAG ACCTACTCGA TTTCTTCGTT CCCAACAAGA ACTAGAAGCT 
AAATTGGAAC AACAACAGTC TGCTGGTGGA GATGCTGAAG GAGGTGGTGA TGATGGTGAT 
GAGGTGCCAC AAATAGATGC TTATGAGCTT TTAGAAGCTG TAGAAATCCT TTCCAAACTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAGAGAAA AGAGGCCCTG 
GAGTCTGTAG AAGTACTAAT AAAAAACCCC AAACTGGAAG CTGGCGATTA TGCAGATTTA 
GTAAAAGCAT TAAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT GGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGGAAGA AATTTGGACA ATATGCAGGA 
CATGTTGTGC CAACCATCTT GGAGAAATTC AAAGAGAAGA AACCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AGCAGACATC TCTTTTTATT 
GCAAGAAGTT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTGCT AAAGCCCTTT 
TGTGCTGCAC TACTTAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCCGCATTT 
GAAGCATTGG GTACTGCTTT GAAGGTGGTT GGCGAGAAAG CAGTAAACCC ATTCCTAGCT 
GATGTGGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAGGT AGAACTGATA 
CATGGTAAGA AAGCTGGACT AGCTGCTGAT AAGAAGGAAT TCAAACCTCT GCCTGGAAGG 
ACTGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACATTTCTGC ACCCAAACCA 
GGACCTCTAA AAAAGGCACC TGCTGCTAAG GCTGGTGGGC CACCAAAAAA GGGGAAACCA 
GCTGCACCAG GAGGCGCAGG GAATACTGGA ACCAAGAACA AGAAAGGACT GGAGACTAAA 
GAAATAGTGG AGCCTGAGCT CTCGATAGAA GTATGTGAAG AAAAAGCTTC AGCTGTTCTT 
CCCCCTACCT GTATACAGCT TCTTGACAGC AGTAACTGGA AAGAAAGGCT GGCTTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAGCTAATG GACCGAACTG AAATGCCATG CCAGGCATTA 
GTGAGGATGC TAGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCATA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC GTCAGCTCAG 
GTTGTATTAG ATGGCCTTGT GGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCAAAA 
GAAGCTATGA CAGCAATAGC CGAAGCCTGT ATGTTACCAT GGACTGCTGA ACAGGTTGTG 
TCAATGGCTT TCTCACAAAA GAATCCCAAA AATCAGTCAG AAACTCTGAA TTGGCTATCA 
AATGCCATAA AAGAATTTGG TTTTTCTGGG TTGAATGTCA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACTG CTGCCATAAC CCTGCTTGGC 
GTGATGTATC TGTATGTTGG TCCCTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAAAGTCC ACCTGCTCCA 
ACCAGAGGAA TTTCCAAGCA TAGCACAAGT GGTACAGATG AAGGAGAAGA TGGAGATGAA 
CCAGATGACG GGAGCAATGA TGTCGTTGAT CTTTTGCCGA GGACGGAGAT CAGTGATAAA 
ATCACTTCAG AGTTGGTATC TAAGATTGGT GATAAGAATT GGAAGATTAG GAAAGAAGGC 
CTAGATGAAG TGGCAGGTAT TATTAATGAC GCAAAATTTA TCCAACCGAA TATAGGTGAA 
CTTCCAACTG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT ACAGCAAACG 
CTGAATATCC TGCAACAACT GGCAGTAGCC ATGGGCCCAA ATATTAAGCA ACATGTAAAA 
AATTTAGGCA TCCCTATCAT CACAGTCCTT GGAGACAGCA AGAACAATGT TCGAGCTGCT 
GCCCTAGCGA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAAGGA 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC TACTCTTCGT TCCACCCCTA CAGACCTTAT CCTTTGTGTT 
CCTCATCTCT ACTCCTGCCT AGAAGATCGA AATGGAGATG TGCGAAAGAA GGCCCAAGAT 
GCCTTGCCAT TCTTCATGAT GCATTTAGGA TATGAAAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAGCCAA CTTCTAAAGA TCAGGTATTG GCCATGCTAG AGAAAGCCAA AGTTAACATG 
CCAGCCAAGC CTGCTCCACC CACTAAAGCA ACTTCTAAAC CAATGGGAGG GTCCGCTCCA 
GCCAAATTCC AGCCTGCATC AGCACCTGCT GAAGATTGTA TTTCCAGCAG TACAGAACCC 
AAACCTGATC CAAAAAAGGC CAAAGCTCCA GGATTATCCT CTAAAGCAAA GAGTGCACAA 
GGGAAGAAGA TGCCAAGCAA AACCAGCTTA AAGGAGGATG AAGACAAATC CGGGCCTATT 
TTTATTGTTG TTCCAAATGG AAAAGAGCAA AGGATGAAAG ATGAAAAAGG ATTGAAGGTG 
CTAAAGTGGA ATTTTACTAC CCCACGGGAT GAATACATTG AGCAACTAAA GACTCAAATG 
TCTAGCTGTG TGGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAAGCCC TTGCTGTTAT GGTTGATCAC TTGGAGAGTG AAAAAGAAGG AGTTATTGGT 
TGCCTGGATC TTATCTTAAA GTGGCTTACC CTGAGGTTTT TTGACACCAA TACAAGCGTC 
CTGATGAAAG CACTAGAATA TTTAAAATTG CTCTTCACCT TGCTAAGTGA AGAAGAATAT 
CATCTTACTG AGAATGAAGC ATCTTCCTTC ATCCCCTATC TTGTCGTCAA GGTTGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTTCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCTAGCA AGATGTTTCC CTTTATCATG GAAGGAACCA AATCCAAAAA CTCTAAGCAG 
AGAGCAGAGT GCCTGGAAGA GCTGGGATGT CTGGTTGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAA ATAGCTGTTC ACATAGGAGA CCGTGACAAT 
GCTGTACGCA ATGCTGCACT CAACACCATT GTAACGGTGT ACAATGTACA TGGGGATCAG 
GTGTTCAAAC TGATTGGAAA TCTTTCTGAA AAGGATATGA GCATGCTCGA GGAGAGGATT 
AAGCGGTCAG CAAAGAGACC CTCTGCTGCA CCAATAAAAC AGGTGGAAGA GAAACCTCAG 
CGTGCACAGA ACATAAGCTC CAATGCCAAC ATGTTACGCA AGGGACCAGC TGAGGACATG 
TCTTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTC 
CGCCGAGAAT TCCAGCTGGA TCTAGATGAG ATTGAGAATG ACAATGGTAC AGTCCGATGT 
GAAATGCCAG AACTTGTTCA GCACAAACTG GATGACATTT TTGAGCCAGT CCTTATTCCT 
GAACCCAAGA TCCGGGCTGT TTCTCCACAC TTCGATGACA TGCACAGTAA TACAGCATCC 
ACAATCAATT TCATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG TATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCCGGCCAT 
ATTGATCAGT TTCTGATAGC CACTTTTATG CAGCTAAGAC TCATCTACAA CACACACATG 
GCAGATGAGA AATTGGAGAA GGACGAGATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAGAGCCTT GCCCGGGAGG CCTCCACTGG AGTACTAAAA 
GACCTAATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GGATTGAAGA TCTTGAGGAA 
GGACAACAGG TCATCCGCTC TGTGAACCTC TTGGTGGTGA AGGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTACTTGTT TTGCTCCAAG ACAGCCTGCT AGCAACAGCC 
AGTTCTCCCA AATTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGAATGGT TCGACTGTTG 
CCTGATACCA TCAATAGCAT TAACCTAGAC AGAATTCTTC TGGATATCCA CATTTTCATG 
AAGGTCTTCC CCAAAGAGAA ACTGAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAGACCCTGC TACACACCTT ATGCAAATTA AAAGGGCCCA AGATCCTGGA CCACCTAACG 
ATGATCGACA ACAAAAACGA GTCTGAGCTG GAGGCCCATC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACTGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATA 
GATGAAAAAT CATCAAAGGC CAAAGTGAAT GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AAAACACTAA AGAGGGACTA GCAGAGTTAT ATGAATATAA GAAGAAATAC 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AATTCCTCAC AGTTCTTCCA GAGCTATGTC 
GAAAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGTCGTAT TTCCACTTCA 
ACAGGCATCT CCCCTCAGAT GGAAGTCACA TGTGTGCCCA CGCCCACAAG CACAGTGTCC 
TCCATAGGTA ACACAAATGG GGAAGAAGTG GGGCCATCTG TCTACTTGGA AAGGCTAAAG 
ATCCTCCGAC AGCGATGTGG TCTGGACAAC ACAAAGCAAG ATGACCGACC TCCTTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCTACT GTCGCCTCTT CCACAGACAT GCTCCACAGC 
AAACTCTCTC AGCTCCGGGA GTCACGGGAG CAGCACCAGC ATTCAGACCT GGATTCTAAC 
CAGACTCACT CTTCAGGAAC TGTGACCTCC TCCTCCTCCA CAGCTAACAT AGACGACTTG 
AAAAAAAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CKAP5 ( NM_001008938.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008938.1 Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 1, mRNA.

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CloneID	OHu19569
Clone ID Related Accession (Same CDS sequence)	NM_001008938.3 , NM_001008938.4
Accession Version	NM_001008938.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	cytoskeleton-associated protein 5 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP282525.1, BC120869.1, D43948.1, CB052595.1, BM980015.1 and AC115088.6. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_001008938.3. Transcript Variant: This variant (1) encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.45127.1, SRR1803612.265289.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000529230.6/ ENSP00000432768.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGGAGATG ACAGTGAGTG GTTGAAACTG CCAGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GGTTAAGTGG GTATGAAGAG GCCCTGAAGA TCTTCCAGAA AATAAAGGAT 
GAAAAGAGCC CAGAGTGGTC CAAATTTTTA GGATTGATCA AAAAATTTGT CACTGATTCC 
AATGCAGTGG TTCAATTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACAGG AGAAGTTGTG TCAGGTGTTG TAAGTAAGGT GTTCAATCAA 
CCTAAAGCTA AAGCCAAGGA GCTGGGCATA GAGATCTGTC TTATGTACAT AGAGATTGAG 
AAAGGAGAGG CTGTTCAAGA AGAGCTCCTG AAAGGCTTGG ACAATAAGAA TCCCAAGATC 
ATAGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTTGGTTC CAAAATCATC 
TTGCTTAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AGTCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAACTAAT TGCTGTGGAG ATTTACAGAT GGATTCGGGA TGCTCTGAGA 
CCCCCATTAC AAAATATAAA CTCTGTTCAG TTGAAAGAAC TAGAAGAAGA ATGGGTCAAA 
CTGCCAACAA GTGCTCCTAG ACCTACTCGA TTTCTTCGTT CCCAACAAGA ACTAGAAGCT 
AAATTGGAAC AACAACAGTC TGCTGGTGGA GATGCTGAAG GAGGTGGTGA TGATGGTGAT 
GAGGTGCCAC AAATAGATGC TTATGAGCTT TTAGAAGCTG TAGAAATCCT TTCCAAACTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAGAGAAA AGAGGCCCTG 
GAGTCTGTAG AAGTACTAAT AAAAAACCCC AAACTGGAAG CTGGCGATTA TGCAGATTTA 
GTAAAAGCAT TAAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT GGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGGAAGA AATTTGGACA ATATGCAGGA 
CATGTTGTGC CAACCATCTT GGAGAAATTC AAAGAGAAGA AACCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AGCAGACATC TCTTTTTATT 
GCAAGAAGTT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTGCT AAAGCCCTTT 
TGTGCTGCAC TACTTAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCCGCATTT 
GAAGCATTGG GTACTGCTTT GAAGGTGGTT GGCGAGAAAG CAGTAAACCC ATTCCTAGCT 
GATGTGGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAGGT AGAACTGATA 
CATGGTAAGA AAGCTGGACT AGCTGCTGAT AAGAAGGAAT TCAAACCTCT GCCTGGAAGG 
ACTGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACATTTCTGC ACCCAAACCA 
GGACCTCTAA AAAAGGCACC TGCTGCTAAG GCTGGTGGGC CACCAAAAAA GGGGAAACCA 
GCTGCACCAG GAGGCGCAGG GAATACTGGA ACCAAGAACA AGAAAGGACT GGAGACTAAA 
GAAATAGTGG AGCCTGAGCT CTCGATAGAA GTATGTGAAG AAAAAGCTTC AGCTGTTCTT 
CCCCCTACCT GTATACAGCT TCTTGACAGC AGTAACTGGA AAGAAAGGCT GGCTTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAGCTAATG GACCGAACTG AAATGCCATG CCAGGCATTA 
GTGAGGATGC TAGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCATA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC GTCAGCTCAG 
GTTGTATTAG ATGGCCTTGT GGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCAAAA 
GAAGCTATGA CAGCAATAGC CGAAGCCTGT ATGTTACCAT GGACTGCTGA ACAGGTTGTG 
TCAATGGCTT TCTCACAAAA GAATCCCAAA AATCAGTCAG AAACTCTGAA TTGGCTATCA 
AATGCCATAA AAGAATTTGG TTTTTCTGGG TTGAATGTCA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACTG CTGCCATAAC CCTGCTTGGC 
GTGATGTATC TGTATGTTGG TCCCTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAAAGTCC ACCTGCTCCA 
ACCAGAGGAA TTTCCAAGCA TAGCACAAGT GGTACAGATG AAGGAGAAGA TGGAGATGAA 
CCAGATGACG GGAGCAATGA TGTCGTTGAT CTTTTGCCGA GGACGGAGAT CAGTGATAAA 
ATCACTTCAG AGTTGGTATC TAAGATTGGT GATAAGAATT GGAAGATTAG GAAAGAAGGC 
CTAGATGAAG TGGCAGGTAT TATTAATGAC GCAAAATTTA TCCAACCGAA TATAGGTGAA 
CTTCCAACTG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT ACAGCAAACG 
CTGAATATCC TGCAACAACT GGCAGTAGCC ATGGGCCCAA ATATTAAGCA ACATGTAAAA 
AATTTAGGCA TCCCTATCAT CACAGTCCTT GGAGACAGCA AGAACAATGT TCGAGCTGCT 
GCCCTAGCGA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAAGGA 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC TACTCTTCGT TCCACCCCTA CAGACCTTAT CCTTTGTGTT 
CCTCATCTCT ACTCCTGCCT AGAAGATCGA AATGGAGATG TGCGAAAGAA GGCCCAAGAT 
GCCTTGCCAT TCTTCATGAT GCATTTAGGA TATGAAAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAGCCAA CTTCTAAAGA TCAGGTATTG GCCATGCTAG AGAAAGCCAA AGTTAACATG 
CCAGCCAAGC CTGCTCCACC CACTAAAGCA ACTTCTAAAC CAATGGGAGG GTCCGCTCCA 
GCCAAATTCC AGCCTGCATC AGCACCTGCT GAAGATTGTA TTTCCAGCAG TACAGAACCC 
AAACCTGATC CAAAAAAGGC CAAAGCTCCA GGATTATCCT CTAAAGCAAA GAGTGCACAA 
GGGAAGAAGA TGCCAAGCAA AACCAGCTTA AAGGAGGATG AAGACAAATC CGGGCCTATT 
TTTATTGTTG TTCCAAATGG AAAAGAGCAA AGGATGAAAG ATGAAAAAGG ATTGAAGGTG 
CTAAAGTGGA ATTTTACTAC CCCACGGGAT GAATACATTG AGCAACTAAA GACTCAAATG 
TCTAGCTGTG TGGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAAGCCC TTGCTGTTAT GGTTGATCAC TTGGAGAGTG AAAAAGAAGG AGTTATTGGT 
TGCCTGGATC TTATCTTAAA GTGGCTTACC CTGAGGTTTT TTGACACCAA TACAAGCGTC 
CTGATGAAAG CACTAGAATA TTTAAAATTG CTCTTCACCT TGCTAAGTGA AGAAGAATAT 
CATCTTACTG AGAATGAAGC ATCTTCCTTC ATCCCCTATC TTGTCGTCAA GGTTGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTTCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCTAGCA AGATGTTTCC CTTTATCATG GAAGGAACCA AATCCAAAAA CTCTAAGCAG 
AGAGCAGAGT GCCTGGAAGA GCTGGGATGT CTGGTTGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAA ATAGCTGTTC ACATAGGAGA CCGTGACAAT 
GCTGTACGCA ATGCTGCACT CAACACCATT GTAACGGTGT ACAATGTACA TGGGGATCAG 
GTGTTCAAAC TGATTGGAAA TCTTTCTGAA AAGGATATGA GCATGCTCGA GGAGAGGATT 
AAGCGGTCAG CAAAGAGACC CTCTGCTGCA CCAATAAAAC AGGTGGAAGA GAAACCTCAG 
CGTGCACAGA ACATAAGCTC CAATGCCAAC ATGTTACGCA AGGGACCAGC TGAGGACATG 
TCTTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTC 
CGCCGAGAAT TCCAGCTGGA TCTAGATGAG ATTGAGAATG ACAATGGTAC AGTCCGATGT 
GAAATGCCAG AACTTGTTCA GCACAAACTG GATGACATTT TTGAGCCAGT CCTTATTCCT 
GAACCCAAGA TCCGGGCTGT TTCTCCACAC TTCGATGACA TGCACAGTAA TACAGCATCC 
ACAATCAATT TCATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG TATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCCGGCCAT 
ATTGATCAGT TTCTGATAGC CACTTTTATG CAGCTAAGAC TCATCTACAA CACACACATG 
GCAGATGAGA AATTGGAGAA GGACGAGATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAGAGCCTT GCCCGGGAGG CCTCCACTGG AGTACTAAAA 
GACCTAATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GGATTGAAGA TCTTGAGGAA 
GGACAACAGG TCATCCGCTC TGTGAACCTC TTGGTGGTGA AGGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTACTTGTT TTGCTCCAAG ACAGCCTGCT AGCAACAGCC 
AGTTCTCCCA AATTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGAATGGT TCGACTGTTG 
CCTGATACCA TCAATAGCAT TAACCTAGAC AGAATTCTTC TGGATATCCA CATTTTCATG 
AAGGTCTTCC CCAAAGAGAA ACTGAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAGACCCTGC TACACACCTT ATGCAAATTA AAAGGGCCCA AGATCCTGGA CCACCTAACG 
ATGATCGACA ACAAAAACGA GTCTGAGCTG GAGGCCCATC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACTGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATA 
GATGAAAAAT CATCAAAGGC CAAAGTGAAT GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AAAACACTAA AGAGGGACTA GCAGAGTTAT ATGAATATAA GAAGAAATAC 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AATTCCTCAC AGTTCTTCCA GAGCTATGTC 
GAAAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGTCGTAT TTCCACTTCA 
ACAGGCATCT CCCCTCAGAT GGAAGTCACA TGTGTGCCCA CGCCCACAAG CACAGTGTCC 
TCCATAGGTA ACACAAATGG GGAAGAAGTG GGGCCATCTG TCTACTTGGA AAGGCTAAAG 
ATCCTCCGAC AGCGATGTGG TCTGGACAAC ACAAAGCAAG ATGACCGACC TCCTTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCTACT GTCGCCTCTT CCACAGACAT GCTCCACAGC 
AAACTCTCTC AGCTCCGGGA GTCACGGGAG CAGCACCAGC ATTCAGACCT GGATTCTAAC 
CAGACTCACT CTTCAGGAAC TGTGACCTCC TCCTCCTCCA CAGCTAACAT AGACGACTTG 
AAAAAAAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008938.1
CDS	99..6197
Translation	MGDDSEWLKLPVDQKCEHKLWKARLSGYEEALKIFQKIKDEKSPEWSKFLGLIKKFVTDSNAVVQLKGLEAALVYVENAHVAGKTTGEVVSGVVSKVFNQPKAKAKELGIEICLMYIEIEKGEAVQEELLKGLDNKNPKIIVACIETLRKALSEFGSKIILLKPIIKVLPKLFESREKAVRDEAKLIAVEIYRWIRDALRPPLQNINSVQLKELEEEWVKLPTSAPRPTRFLRSQQELEAKLEQQQSAGGDAEGGGDDGDEVPQIDAYELLEAVEILSKLPKDFYDKIEAKKWQERKEALESVEVLIKNPKLEAGDYADLVKALKKVVGKDTNVMLVALAAKCLTGLAVGLRKKFGQYAGHVVPTILEKFKEKKPQVVQALQEAIDAIFLTTTLQNISEDVLAVMDNKNPTIKQQTSLFIARSFRHCTASTLPKSLLKPFCAALLKHINDSAPEVRDAAFEALGTALKVVGEKAVNPFLADVDKLKLDKIKECSEKVELIHGKKAGLAADKKEFKPLPGRTAASGAAGDKDTKDISAPKPGPLKKAPAAKAGGPPKKGKPAAPGGAGNTGTKNKKGLETKEIVEPELSIEVCEEKASAVLPPTCIQLLDSSNWKERLACMEEFQKAVELMDRTEMPCQALVRMLAKKPGWKETNFQVMQMKLHIVALIAQKGNFSKTSAQVVLDGLVDKIGDVKCGNNAKEAMTAIAEACMLPWTAEQVVSMAFSQKNPKNQSETLNWLSNAIKEFGFSGLNVKAFISNVKTALAATNPAVRTAAITLLGVMYLYVGPSLRMFFEDEKPALLSQIDAEFEKMQGQSPPAPTRGISKHSTSGTDEGEDGDEPDDGSNDVVDLLPRTEISDKITSELVSKIGDKNWKIRKEGLDEVAGIINDAKFIQPNIGELPTALKGRLNDSNKILVQQTLNILQQLAVAMGPNIKQHVKNLGIPIITVLGDSKNNVRAAALATVNAWAEQTGMKEWLEGEDLSEELKKENPFLRQELLGWLAEKLPTLRSTPTDLILCVPHLYSCLEDRNGDVRKKAQDALPFFMMHLGYEKMAKATGKLKPTSKDQVLAMLEKAKVNMPAKPAPPTKATSKPMGGSAPAKFQPASAPAEDCISSSTEPKPDPKKAKAPGLSSKAKSAQGKKMPSKTSLKEDEDKSGPIFIVVPNGKEQRMKDEKGLKVLKWNFTTPRDEYIEQLKTQMSSCVAKWLQDEMFHSDFQHHNKALAVMVDHLESEKEGVIGCLDLILKWLTLRFFDTNTSVLMKALEYLKLLFTLLSEEEYHLTENEASSFIPYLVVKVGEPKDVIRKDVRAILNRMCLVYPASKMFPFIMEGTKSKNSKQRAECLEELGCLVESYGMNVCQPTPGKALKEIAVHIGDRDNAVRNAALNTIVTVYNVHGDQVFKLIGNLSEKDMSMLEERIKRSAKRPSAAPIKQVEEKPQRAQNISSNANMLRKGPAEDMSSKLNQARSMSGHPEAAQMVRREFQLDLDEIENDNGTVRCEMPELVQHKLDDIFEPVLIPEPKIRAVSPHFDDMHSNTASTINFIISQVASGDINTSIQALTQIDEVLRQEDKAEAMSGHIDQFLIATFMQLRLIYNTHMADEKLEKDEIIKLYSCIIGNMISLFQIESLAREASTGVLKDLMHGLITLMLDSRIEDLEEGQQVIRSVNLLVVKVLEKSDQTNILSALLVLLQDSLLATASSPKFSELVMKCLWRMVRLLPDTINSINLDRILLDIHIFMKVFPKEKLKQCKSEFPIRTLKTLLHTLCKLKGPKILDHLTMIDNKNESELEAHLCRMMKHSMDQTGSKSDKETEKGASRIDEKSSKAKVNDFLAEIFKKIGSKENTKEGLAELYEYKKKYSDADIEPFLKNSSQFFQSYVERGLRVIEMEREGKGRISTSTGISPQMEVTCVPTPTSTVSSIGNTNGEEVGPSVYLERLKILRQRCGLDNTKQDDRPPLTSLLSKPAVPTVASSTDMLHSKLSQLRESREQHQHSDLDSNQTHSSGTVTSSSSTANIDDLKKRLERIKSSRK

Target ORF information:

RefSeq Version	NM_001008938.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytoskeleton associated protein 5 (CKAP5), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008938.4

ORF Insert Sequence:

ATGGGAGATG ACAGTGAGTG GTTGAAACTG CCAGTTGATC AGAAATGTGA ACACAAGCTG 
TGGAAAGCAA GGTTAAGTGG GTATGAAGAG GCCCTGAAGA TCTTCCAGAA AATAAAGGAT 
GAAAAGAGCC CAGAGTGGTC CAAATTTTTA GGATTGATCA AAAAATTTGT CACTGATTCC 
AATGCAGTGG TTCAATTGAA AGGATTAGAA GCTGCACTTG TTTATGTTGA AAATGCCCAT 
GTAGCAGGAA AAACCACAGG AGAAGTTGTG TCAGGTGTTG TAAGTAAGGT GTTCAATCAA 
CCTAAAGCTA AAGCCAAGGA GCTGGGCATA GAGATCTGTC TTATGTACAT AGAGATTGAG 
AAAGGAGAGG CTGTTCAAGA AGAGCTCCTG AAAGGCTTGG ACAATAAGAA TCCCAAGATC 
ATAGTGGCCT GTATAGAGAC ACTGAGGAAA GCCTTAAGTG AATTTGGTTC CAAAATCATC 
TTGCTTAAGC CAATTATCAA AGTGTTGCCA AAACTCTTTG AGTCTCGAGA GAAGGCTGTT 
CGAGATGAAG CCAAACTAAT TGCTGTGGAG ATTTACAGAT GGATTCGGGA TGCTCTGAGA 
CCCCCATTAC AAAATATAAA CTCTGTTCAG TTGAAAGAAC TAGAAGAAGA ATGGGTCAAA 
CTGCCAACAA GTGCTCCTAG ACCTACTCGA TTTCTTCGTT CCCAACAAGA ACTAGAAGCT 
AAATTGGAAC AACAACAGTC TGCTGGTGGA GATGCTGAAG GAGGTGGTGA TGATGGTGAT 
GAGGTGCCAC AAATAGATGC TTATGAGCTT TTAGAAGCTG TAGAAATCCT TTCCAAACTT 
CCCAAAGACT TTTATGACAA AATTGAGGCA AAAAAATGGC AAGAGAGAAA AGAGGCCCTG 
GAGTCTGTAG AAGTACTAAT AAAAAACCCC AAACTGGAAG CTGGCGATTA TGCAGATTTA 
GTAAAAGCAT TAAAGAAGGT TGTTGGAAAG GACACCAATG TCATGTTGGT GGCTTTGGCA 
GCAAAATGTC TTACTGGCCT GGCTGTTGGG CTAAGGAAGA AATTTGGACA ATATGCAGGA 
CATGTTGTGC CAACCATCTT GGAGAAATTC AAAGAGAAGA AACCTCAAGT GGTACAAGCC 
CTGCAGGAGG CAATTGATGC AATCTTCCTT ACTACCACAC TACAGAACAT CAGTGAGGAT 
GTTTTAGCAG TAATGGATAA TAAAAATCCA ACCATCAAGC AGCAGACATC TCTTTTTATT 
GCAAGAAGTT TCCGCCACTG CACTGCTTCT ACCCTGCCAA AGAGCTTGCT AAAGCCCTTT 
TGTGCTGCAC TACTTAAGCA CATCAATGAT TCTGCTCCTG AAGTCAGAGA TGCCGCATTT 
GAAGCATTGG GTACTGCTTT GAAGGTGGTT GGCGAGAAAG CAGTAAACCC ATTCCTAGCT 
GATGTGGACA AACTCAAGCT TGATAAGATC AAAGAATGTT CAGAAAAGGT AGAACTGATA 
CATGGTAAGA AAGCTGGACT AGCTGCTGAT AAGAAGGAAT TCAAACCTCT GCCTGGAAGG 
ACTGCTGCTT CAGGGGCTGC AGGAGATAAG GACACAAAGG ACATTTCTGC ACCCAAACCA 
GGACCTCTAA AAAAGGCACC TGCTGCTAAG GCTGGTGGGC CACCAAAAAA GGGGAAACCA 
GCTGCACCAG GAGGCGCAGG GAATACTGGA ACCAAGAACA AGAAAGGACT GGAGACTAAA 
GAAATAGTGG AGCCTGAGCT CTCGATAGAA GTATGTGAAG AAAAAGCTTC AGCTGTTCTT 
CCCCCTACCT GTATACAGCT TCTTGACAGC AGTAACTGGA AAGAAAGGCT GGCTTGTATG 
GAAGAGTTCC AGAAGGCTGT TGAGCTAATG GACCGAACTG AAATGCCATG CCAGGCATTA 
GTGAGGATGC TAGCCAAGAA ACCTGGATGG AAAGAAACTA ATTTTCAGGT GATGCAAATG 
AAGCTTCATA TAGTTGCTTT GATTGCCCAG AAGGGAAATT TTTCCAAAAC GTCAGCTCAG 
GTTGTATTAG ATGGCCTTGT GGACAAGATT GGAGATGTGA AATGTGGGAA CAATGCAAAA 
GAAGCTATGA CAGCAATAGC CGAAGCCTGT ATGTTACCAT GGACTGCTGA ACAGGTTGTG 
TCAATGGCTT TCTCACAAAA GAATCCCAAA AATCAGTCAG AAACTCTGAA TTGGCTATCA 
AATGCCATAA AAGAATTTGG TTTTTCTGGG TTGAATGTCA AAGCTTTCAT TAGCAATGTG 
AAGACAGCTC TTGCTGCAAC AAACCCAGCT GTGAGGACTG CTGCCATAAC CCTGCTTGGC 
GTGATGTATC TGTATGTTGG TCCCTCTTTG CGAATGTTCT TTGAGGATGA GAAGCCTGCC 
CTCCTATCCC AGATAGATGC AGAATTTGAG AAGATGCAGG GACAAAGTCC ACCTGCTCCA 
ACCAGAGGAA TTTCCAAGCA TAGCACAAGT GGTACAGATG AAGGAGAAGA TGGAGATGAA 
CCAGATGACG GGAGCAATGA TGTCGTTGAT CTTTTGCCGA GGACGGAGAT CAGTGATAAA 
ATCACTTCAG AGTTGGTATC TAAGATTGGT GATAAGAATT GGAAGATTAG GAAAGAAGGC 
CTAGATGAAG TGGCAGGTAT TATTAATGAC GCAAAATTTA TCCAACCGAA TATAGGTGAA 
CTTCCAACTG CCTTGAAGGG TCGACTCAAT GATTCAAATA AAATCTTGGT ACAGCAAACG 
CTGAATATCC TGCAACAACT GGCAGTAGCC ATGGGCCCAA ATATTAAGCA ACATGTAAAA 
AATTTAGGCA TCCCTATCAT CACAGTCCTT GGAGACAGCA AGAACAATGT TCGAGCTGCT 
GCCCTAGCGA CTGTGAATGC TTGGGCAGAA CAGACTGGCA TGAAGGAATG GCTGGAAGGA 
GAAGATCTTT CTGAAGAGCT CAAAAAGGAA AATCCTTTCT TGAGGCAAGA GCTTCTGGGC 
TGGCTGGCTG AGAAACTACC TACTCTTCGT TCCACCCCTA CAGACCTTAT CCTTTGTGTT 
CCTCATCTCT ACTCCTGCCT AGAAGATCGA AATGGAGATG TGCGAAAGAA GGCCCAAGAT 
GCCTTGCCAT TCTTCATGAT GCATTTAGGA TATGAAAAAA TGGCCAAGGC TACTGGGAAA 
CTAAAGCCAA CTTCTAAAGA TCAGGTATTG GCCATGCTAG AGAAAGCCAA AGTTAACATG 
CCAGCCAAGC CTGCTCCACC CACTAAAGCA ACTTCTAAAC CAATGGGAGG GTCCGCTCCA 
GCCAAATTCC AGCCTGCATC AGCACCTGCT GAAGATTGTA TTTCCAGCAG TACAGAACCC 
AAACCTGATC CAAAAAAGGC CAAAGCTCCA GGATTATCCT CTAAAGCAAA GAGTGCACAA 
GGGAAGAAGA TGCCAAGCAA AACCAGCTTA AAGGAGGATG AAGACAAATC CGGGCCTATT 
TTTATTGTTG TTCCAAATGG AAAAGAGCAA AGGATGAAAG ATGAAAAAGG ATTGAAGGTG 
CTAAAGTGGA ATTTTACTAC CCCACGGGAT GAATACATTG AGCAACTAAA GACTCAAATG 
TCTAGCTGTG TGGCTAAATG GTTACAAGAT GAGATGTTTC ACTCAGACTT TCAGCATCAT 
AACAAAGCCC TTGCTGTTAT GGTTGATCAC TTGGAGAGTG AAAAAGAAGG AGTTATTGGT 
TGCCTGGATC TTATCTTAAA GTGGCTTACC CTGAGGTTTT TTGACACCAA TACAAGCGTC 
CTGATGAAAG CACTAGAATA TTTAAAATTG CTCTTCACCT TGCTAAGTGA AGAAGAATAT 
CATCTTACTG AGAATGAAGC ATCTTCCTTC ATCCCCTATC TTGTCGTCAA GGTTGGAGAA 
CCAAAGGATG TCATTCGTAA AGATGTTCGT GCCATCCTGA ACCGGATGTG CCTTGTCTAC 
CCAGCTAGCA AGATGTTTCC CTTTATCATG GAAGGAACCA AATCCAAAAA CTCTAAGCAG 
AGAGCAGAGT GCCTGGAAGA GCTGGGATGT CTGGTTGAGT CCTATGGCAT GAATGTTTGC 
CAACCAACCC CAGGAAAAGC CTTAAAGGAA ATAGCTGTTC ACATAGGAGA CCGTGACAAT 
GCTGTACGCA ATGCTGCACT CAACACCATT GTAACGGTGT ACAATGTACA TGGGGATCAG 
GTGTTCAAAC TGATTGGAAA TCTTTCTGAA AAGGATATGA GCATGCTCGA GGAGAGGATT 
AAGCGGTCAG CAAAGAGACC CTCTGCTGCA CCAATAAAAC AGGTGGAAGA GAAACCTCAG 
CGTGCACAGA ACATAAGCTC CAATGCCAAC ATGTTACGCA AGGGACCAGC TGAGGACATG 
TCTTCCAAAC TCAACCAAGC CCGAAGCATG AGTGGGCATC CTGAGGCAGC CCAGATGGTC 
CGCCGAGAAT TCCAGCTGGA TCTAGATGAG ATTGAGAATG ACAATGGTAC AGTCCGATGT 
GAAATGCCAG AACTTGTTCA GCACAAACTG GATGACATTT TTGAGCCAGT CCTTATTCCT 
GAACCCAAGA TCCGGGCTGT TTCTCCACAC TTCGATGACA TGCACAGTAA TACAGCATCC 
ACAATCAATT TCATTATCTC CCAAGTAGCC AGTGGTGACA TCAACACAAG TATCCAAGCT 
CTGACACAGA TCGATGAGGT CCTGAGACAG GAAGACAAAG CTGAAGCCAT GTCCGGCCAT 
ATTGATCAGT TTCTGATAGC CACTTTTATG CAGCTAAGAC TCATCTACAA CACACACATG 
GCAGATGAGA AATTGGAGAA GGACGAGATC ATCAAGTTGT ATAGCTGTAT CATTGGCAAC 
ATGATTTCGC TGTTTCAGAT AGAGAGCCTT GCCCGGGAGG CCTCCACTGG AGTACTAAAA 
GACCTAATGC ATGGCCTCAT CACCTTAATG CTGGATTCTC GGATTGAAGA TCTTGAGGAA 
GGACAACAGG TCATCCGCTC TGTGAACCTC TTGGTGGTGA AGGTTCTGGA GAAGTCAGAC 
CAGACCAACA TCCTGAGTGC CCTACTTGTT TTGCTCCAAG ACAGCCTGCT AGCAACAGCC 
AGTTCTCCCA AATTCTCAGA GCTTGTTATG AAGTGTCTCT GGAGAATGGT TCGACTGTTG 
CCTGATACCA TCAATAGCAT TAACCTAGAC AGAATTCTTC TGGATATCCA CATTTTCATG 
AAGGTCTTCC CCAAAGAGAA ACTGAAGCAA TGCAAAAGTG AATTTCCCAT AAGGACCCTA 
AAGACCCTGC TACACACCTT ATGCAAATTA AAAGGGCCCA AGATCCTGGA CCACCTAACG 
ATGATCGACA ACAAAAACGA GTCTGAGCTG GAGGCCCATC TCTGCCGGAT GATGAAGCAC 
AGTATGGACC AGACTGGGAG CAAGTCTGAT AAGGAAACAG AAAAGGGAGC ATCTCGAATA 
GATGAAAAAT CATCAAAGGC CAAAGTGAAT GATTTCTTAG CTGAGATTTT TAAGAAGATT 
GGCTCTAAAG AAAACACTAA AGAGGGACTA GCAGAGTTAT ATGAATATAA GAAGAAATAC 
TCAGATGCTG ACATTGAACC ATTTCTGAAA AATTCCTCAC AGTTCTTCCA GAGCTATGTC 
GAAAGAGGCC TTCGGGTGAT TGAGATGGAG AGGGAGGGCA AAGGTCGTAT TTCCACTTCA 
ACAGGCATCT CCCCTCAGAT GGAAGTCACA TGTGTGCCCA CGCCCACAAG CACAGTGTCC 
TCCATAGGTA ACACAAATGG GGAAGAAGTG GGGCCATCTG TCTACTTGGA AAGGCTAAAG 
ATCCTCCGAC AGCGATGTGG TCTGGACAAC ACAAAGCAAG ATGACCGACC TCCTTTGACC 
TCTTTGCTCT CCAAACCAGC AGTTCCTACT GTCGCCTCTT CCACAGACAT GCTCCACAGC 
AAACTCTCTC AGCTCCGGGA GTCACGGGAG CAGCACCAGC ATTCAGACCT GGATTCTAAC 
CAGACTCACT CTTCAGGAAC TGTGACCTCC TCCTCCTCCA CAGCTAACAT AGACGACTTG 
AAAAAAAGAC TGGAGAGAAT AAAGAGCAGT CGCAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Eribulin targets a ch-TOG-dependent directed migration of cancer cells.
Oncotarget6(39)41667-78(2015 Dec)
Chanez B,Gon?alves A,Badache A,Verdier-Pinard P

Complementary activities of TPX2 and chTOG constitute an efficient importin-regulated microtubule nucleation module.
Nature cell biology17(11)1422-34(2015 Nov)
Roostalu J,Cade NI,Surrey T

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

TTBK2 with EB1/3 regulates microtubule dynamics in migrating cells through KIF2A phosphorylation.
The Journal of cell biology210(5)737-51(2015 Aug)
Watanabe T,Kakeno M,Matsui T,Sugiyama I,Arimura N,Matsuzawa K,Shirahige A,Ishidate F,Nishioka T,Taya S,Hoshino M,Kaibuchi K

TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation.
Biology open4(2)170-9(2015 Jan)
Guti?rrez-Caballero C,Burgess SG,Bayliss R,Royle SJ

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CKAP5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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