DCAF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DCAF1
Entrez Gene ID	9730
Full Name	DDB1 and CUL4 associated factor 1
Synonyms	RIP,VPRBP
General protein information	Preferred Names DDB1 and CUL4 associated factor 1 Names DDB1- and CUL4-associated factor 1 protein VPRBP HIV-1 Vpr-binding protein Vpr (HIV-1) binding protein Vpr-binding protein serine/threonine-protein kinase VPRBP vpr-interacting protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.2
Disorder MIM:	617259

mRNA and Protein(s)

mRNA	Protein	Name
XM_017007547.1	XP_016863036.1	protein VPRBP isoform X1
XM_017007549.1	XP_016863038.1	protein VPRBP isoform X1
NM_001349171.2	NP_001336100.1	DDB1- and CUL4-associated factor 1 isoform 3
XM_017007547.2	XP_016863036.1	DDB1- and CUL4-associated factor 1 isoform X1
NM_014703.2	NP_055518.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_014703.3	NP_055518.1	DDB1- and CUL4-associated factor 1 isoform 1
XM_017007550.2	XP_016863039.1	DDB1- and CUL4-associated factor 1 isoform X1
NM_001349170.1	NP_001336099.1	DDB1- and CUL4-associated factor 1 isoform 1
XM_005276753.4	XP_005276810.1	protein VPRBP isoform X1
XM_017007550.1	XP_016863039.1	protein VPRBP isoform X1
XM_011534274.2	XP_011532576.1	protein VPRBP isoform X1
XM_011534273.2	XP_011532575.1	protein VPRBP isoform X1
XM_017007546.1	XP_016863035.1	protein VPRBP isoform X1
NM_001349171.1	NP_001336100.1	DDB1- and CUL4-associated factor 1 isoform 3
NM_001349168.2	NP_001336097.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001349170.2	NP_001336099.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001349169.2	NP_001336098.1	DDB1- and CUL4-associated factor 1 isoform 1
XM_005276755.5	XP_005276812.1	DDB1- and CUL4-associated factor 1 isoform X1
NM_001171904.1	NP_001165375.1	DDB1- and CUL4-associated factor 1 isoform 2
XM_005276755.4	XP_005276812.1	protein VPRBP isoform X1
XM_011534275.2	XP_011532577.1	protein VPRBP isoform X1
NM_001349168.1	NP_001336097.1	DDB1- and CUL4-associated factor 1 isoform 1
NM_001349169.1	NP_001336098.1	DDB1- and CUL4-associated factor 1 isoform 1
XM_011534273.3	XP_011532575.1	DDB1- and CUL4-associated factor 1 isoform X1
XM_005276753.5	XP_005276810.1	DDB1- and CUL4-associated factor 1 isoform X1
XM_017007549.2	XP_016863038.1	DDB1- and CUL4-associated factor 1 isoform X1
XM_017007546.2	XP_016863035.1	DDB1- and CUL4-associated factor 1 isoform X1
XM_011534275.3	XP_011532577.1	DDB1- and CUL4-associated factor 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	VPRBP	XP_005632545.1
Drosophila melanogaster (fruit fly)	mahj	NP_001246451.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012134	XP_320393.4
Mus musculus (house mouse)	Vprbp	NP_001015507.1
Rattus norvegicus (Norway rat)	Vprbp	NP_001178727.1
Gallus gallus (chicken)	VPRBP	XP_004944460.1
Xenopus tropicalis (tropical clawed frog)	vprbp	XP_004914132.1
Bos taurus (cattle)	VPRBP	XP_005223061.1
Caenorhabditis elegans (roundworm)	dcaf-1	NP_501725.2
Homo sapiens (human)	VPRBP	NP_055518.1
Pan troglodytes (chimpanzee)	VPRBP	XP_001169544.2

Gene Ontology
Bibliography

Related articles in PubMed

DCAF1 is involved in HCV replication through regulation of miR-122.
Yan Y, Li C, Sun B, Yang R
Archives of virology(2018 Jan)

HIV-1 Vpr protein directly loads helicase-like transcription factor (HLTF) onto the CRL4-DCAF1 E3 ubiquitin ligase.
Zhou X, DeLucia M, Hao C, Hrecka K, Monnie C, Skowronski J, Ahn J
The Journal of biological chemistry292(51)21117-21127(2017 Dec)

VprBP/DCAF1 Regulates the Degradation and Nonproteolytic Activation of the Cell Cycle Transcription Factor FoxM1.
Wang X, Arceci A, Bird K, Mills CA, Choudhury R, Kernan JL, Zhou C, Bae-Jump V, Bowers A, Emanuele MJ
Molecular and cellular biology37(13)(2017 Jul)

Virion-Associated Vpr Alleviates a Postintegration Block to HIV-1 Infection of Dendritic Cells.
Miller CM, Akiyama H, Agosto LM, Emery A, Ettinger CR, Swanstrom RI, Henderson AJ, Gummuluru S
Journal of virology91(13)(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High VPRBP expression is associated with high-grade serous ovarian cancer.
Title: VprBP/DCAF1 Regulates the Degradation and Nonproteolytic Activation of the Cell Cycle Transcription Factor FoxM1.

Together, these results characterize a novel postintegration restriction of HIV-1 replication in monocyte-derived dendritic cells and show that the interaction of Vpr with the DCAF1/DDB1 E3 ubiquitin ligase complex and the yet-to-be-identified host factor might alleviate this restriction by inducing transcription from the viral long terminal repeat.
Title: Virion-Associated Vpr Alleviates a Postintegration Block to HIV-1 Infection of Dendritic Cells.

RNA interference-mediated knockdown of differentially regulated host factors identified Vpr binding protein (VprBP) as proviral host factor because its downregulation inhibited efficient propagation of seasonal influenza A virus.
Title: Quantitative Proteomic Approach Identifies Vpr Binding Protein as Novel Host Factor Supporting Influenza A Virus Infections in Human Cells.

This study presents the crystal structure of the DDB1-DCAF1-HIV-1-Vpr-uracil-DNA glycosylase (cyclin U) complex.
Title: The DDB1-DCAF1-Vpr-UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction.

Data show that neurofibromin 2 (Merlin) suppresses proliferation and adhesion, at least partly, through inhibiting kinase suppressor of Ras 1 (KSR1) and DCAF1 protein.
Title: The scaffold protein KSR1, a novel therapeutic target for the treatment of Merlin-deficient tumors.

The following DCAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DCAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13867	NM_014703.3 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	NM_001349169.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	NM_001349168.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	NM_001349170.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007549.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007546.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_005276755.5 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007547.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007550.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_011534274.2 Latest version!	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_011534275.3 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_005276753.5 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_011534273.3 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu14003	NM_001171904.1 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu37617	NM_001349171.2 Latest version!	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu13867	XM_017007547.1	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	NM_014703.2	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007550.1	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu37617	NM_001349171.1	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu13867	NM_001349170.1	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_005276755.4	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	NM_001349168.1	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	NM_001349169.1	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_011534273.2	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_005276753.4	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007549.1	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_017007546.1	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu13867	XM_011534275.2	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DCAF1 ( NM_014703.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055518.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_014703.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR986856.1 and AB018343.1. On Jan 28, 2010 this sequence version replaced NM_014703.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1, 3, 4, and 5 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018343.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055518.1
CDS	186..4709
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_014703.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014703.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_014703.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055518.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 1, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_014703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2, CR986856.1 and AB018343.1. On Jun 2, 2019 this sequence version replaced NM_014703.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1, 3, 4, and 5 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB018343.1, SRR7346977.2313698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055518.1
CDS	199..4722
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_014703.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014703.3

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349170.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336099.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 5, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_001349170.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Mar 9, 2017 this sequence version replaced XM_011534277.2. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs compared to variant 1. Variants 1, 3, 4, and 5 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.94032.1, SRR1803614.96745.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336099.1
CDS	139..4662
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349170.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349170.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349168.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336097.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_001349168.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Jun 3, 2019 this sequence version replaced NM_001349168.1. Transcript Variant: This variant (3) differs in the 5' and 3' UTRs compared to variant 1. Variants 1, 3, 4, and 5 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.198673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336097.1
CDS	252..4775
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349168.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349168.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349170.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336099.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_001349170.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Jun 2, 2019 this sequence version replaced NM_001349170.1. Transcript Variant: This variant (5) differs in the 5' and 3' UTRs compared to variant 1. Variants 1, 3, 4, and 5 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.94032.1, SRR1803616.275629.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336099.1
CDS	152..4675
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349170.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349170.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349169.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336098.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 4, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_001349169.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Jun 2, 2019 this sequence version replaced NM_001349169.1. Transcript Variant: This variant (4) differs in the 5' and 3' UTRs compared to variant 1. Variants 1, 3, 4, and 5 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.34750.1, SRR1660809.249885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336098.1
CDS	299..4822
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349169.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349169.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349168.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336097.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 3, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_001349168.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Mar 9, 2017 this sequence version replaced XM_017007548.1. Transcript Variant: This variant (3) differs in the 5' and 3' UTRs compared to variant 1. Variants 1, 3, 4, and 5 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.198673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336097.1
CDS	239..4762
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349168.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349168.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349169.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336098.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 4, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	NM_001349169.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Mar 9, 2017 this sequence version replaced XM_011534276.2. Transcript Variant: This variant (4) differs in the 5' and 3' UTRs compared to variant 1. Variants 1, 3, 4, and 5 all encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.34750.1, SRR1660809.249885.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336098.1
CDS	286..4809
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349169.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349169.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007547.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863036.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007547.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863036.1
CDS	152..4675
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007547.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007547.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007549.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863038.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007549.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863038.1
CDS	337..4860
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007549.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007549.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007547.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863036.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X3, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017007547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863036.1
CDS	152..4675
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007547.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007547.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007550.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863039.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X8, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007550.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017007550.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863039.1
CDS	338..4861
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007550.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007550.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005276753.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005276810.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X10, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_005276753.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005276753.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005276810.1
CDS	17..4540
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005276753.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005276753.4

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007550.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863039.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X12, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007550.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863039.1
CDS	337..4860
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007550.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007550.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_011534274.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532576.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_011534274.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534274.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532576.1
CDS	337..4860
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_011534274.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534274.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_011534273.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532575.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_011534273.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534273.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532575.1
CDS	277..4800
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_011534273.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534273.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007546.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863035.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007546.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863035.1
CDS	195..4718
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007546.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007546.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005276755.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005276812.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X5, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_005276755.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005276755.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005276812.1
CDS	98..4621
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005276755.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005276755.5

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005276755.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005276812.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X9, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_005276755.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005276755.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005276812.1
CDS	98..4621
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005276755.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005276755.4

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_011534275.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532577.1 Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X7, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_011534275.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein VPRBP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534275.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532577.1
CDS	252..4775
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_011534275.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Vpr (HIV-1) binding protein (VPRBP), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534275.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_011534273.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532575.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X1, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_011534273.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534273.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532575.1
CDS	277..4800
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_011534273.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534273.3

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_005276753.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005276810.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X6, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_005276753.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005276753.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005276810.1
CDS	17..4540
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_005276753.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005276753.5

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007549.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863038.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X7, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007549.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017007549.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863038.1
CDS	338..4861
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007549.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007549.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_017007546.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863035.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X2, mRNA.

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CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_017007546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017007546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863035.1
CDS	195..4718
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_017007546.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007546.2

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( XM_011534275.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532577.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu13867
Clone ID Related Accession (Same CDS sequence)	XM_017007547.1 , XM_017007549.1 , XM_017007547.2 , NM_014703.2 , NM_014703.3 , XM_017007550.2 , NM_001349170.1 , XM_005276753.4 , XM_017007550.1 , XM_011534274.2 , XM_011534273.2 , XM_017007546.1 , NM_001349168.2 , NM_001349170.2 , NM_001349169.2 , XM_005276755.5 , XM_005276755.4 , XM_011534275.2 , NM_001349168.1 , NM_001349169.1 , XM_011534273.3 , XM_005276753.5 , XM_017007549.2 , XM_017007546.2 , XM_011534275.3
Accession Version	XM_011534275.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4524bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011534275.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532577.1
CDS	252..4775
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNALVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	XM_011534275.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534275.3

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATGC ACTGGTGAAT GCATATGTGA TGACAAGCCG AGAGCCCCCT 
TTAAACACTG CAGCTTGCAG ACTCCTATTA GACATCATGC CAGGGCTGGA AACTGCTGTC 
GTCTTTCAAG AAAAGGAGGG AATTGTCGAG AATCTTTTCA AATGGGCCCG AGAGGCCGAT 
CAACCATTGA GGACATATTC TACTGGACTG TTAGGAGGTG CTATGGAAAA TCAAGACATT 
GCTGCCAACT ATAGAGATGA AAATTCACAG CTGGTGGCAA TAGTGCTTCG AAGACTGAGG 
GAGCTACAGC TACAGGAAGT GGCTTTGCGG CAGGAAAACA AGCGTCCCAG TCCACGGAAG 
CTCTCTTCTG AACCCCTTTT GCCTCTGGAT GAGGAGGCTG TGGATATGGA CTATGGTGAC 
ATGGCTGTAG ATGTAGTGGA TGGAGACCAA GAGGAAGCTT CTGGAGACAT GGAGATCTCC 
TTTCATCTTG ATTCAGGCCA CAAGACTAGT AGCAGAGTGA ACTCAACAAC CAAACCTGAG 
GATGGAGGAT TAAAGAAAAA CAAGTCAGCA AAACAGGGTG ACAGAGAGAA CTTTAGGAAA 
GCCAAGCAAA AGTTGGGTTT CTCATCTTCT GATCCAGATC GCATGTTTGT TGAGCTGTCT 
AATAGCAGTT GGTCAGAAAT GTCTCCCTGG GTGATTGGCA CCAATTATAC CCTTTATCCT 
ATGACTCCTG CTATCGAGCA GCGACTCATT CTCCAATATT TGACCCCTCT AGGAGAATAT 
CAGGAGCTAC TTCCCATATT CATGCAACTT GGATCACGGG AGCTGATGAT GTTCTATATT 
GACCTGAAGC AAACTAATGA TGTCCTGCTT ACATTTGAGG CACTAAAGCA CCTAGCATCT 
CTCCTGCTCC ATAACAAATT TGCCACAGAA TTTGTTGCGC ATGGTGGAGT ACAGAAATTA 
CTGGAAATAC CTCGTCCTTC TATGGCTGCA ACCGGTGTGT CTATGTGTTT GTATTACCTA 
TCCTACAATC AGGATGCCAT GGAAAGAGTT TGCATGCATC CCCACAATGT TCTGTCTGAT 
GTGGTGAACT ATACCCTGTG GTTAATGGAG TGTTCTCATG CTTCAGGATG CTGCCATGCT 
ACCATGTTTT TTTCAATTTG CTTCTCATTT CGGGCCGTCT TGGAGCTCTT TGACCGCTAT 
GATGGTCTTC GTCGTCTGGT GAACTTGATC AGTACTTTGG AGATTCTAAA TTTGGAAGAT 
CAGGGTGCAC TTCTGAGTGA TGATGAAATA TTTGCTAGCC GCCAAACTGG GAAACATACC 
TGCATGGCCT TGCGCAAATA CTTTGAGGCT CACCTGGCCA TTAAATTGGA ACAAGTGAAG 
CAGTCACTTC AGAGGACTGA GGGTGGCATT CTTGTCCACC CACAACCCCC GTACAAGGCA 
TGCTCATATA CTCATGAACA GATTGTGGAA ATGATGGAAT TTTTGATAGA ATATGGCCCA 
GCGCAGCTAT ATTGGGAACC AGCTGAAGTT TTCCTCAAAC TTTCTTGTGT GCAACTCTTG 
TTGCAGCTTA TTTCTATTGC CTGCAATTGG AAGACCTATT ATGCAAGGAA TGACACTGTG 
CGCTTTGCTT TGGATGTCCT GGCTATTCTT ACTGTGGTGC CAAAAATCCA GCTCCAGTTG 
GCAGAATCAG TGGACGTGTT GGATGAGGCT GGATCTACAG TCTCTACTGT AGGTATCAGC 
ATTATTTTGG GAGTGGCTGA GGGTGAGTTC TTCATCCATG ATGCTGAAAT TCAGAAGTCA 
GCACTTCAGA TTATCATCAA TTGTGTGTGT GGCCCAGATA ACCGAATATC CAGTATTGGT 
AAATTTATCT CTGGTACTCC TCGGAGAAAG CTGCCTCAGA ACCCTAAAAG CAGTGAGCAC 
ACCCTGGCCA AGATGTGGAA TGTGGTTCAG TCCAACAACG GCATCAAGGT GCTCCTGTCC 
TTACTGTCCA TTAAGATGCC CATCACAGAT GCAGACCAAA TCCGGGCCCT GGCCTGCAAA 
GCCCTAGTGG GCCTGTCTCG CAGTAGCACT GTCCGGCAGA TCATCAGTAA ACTGCCCCTT 
TTCAGCAGCT GCCAGATCCA GCAGCTGATG AAGGAGCCTG TGCTGCAGGA CAAGCGCAGT 
GACCATGTCA AGTTCTGCAA GTATGCTGCT GAACTCATTG AACGGGTGTC AGGAAAACCA 
CTTCTCATTG GCACTGATGT TTCCCTAGCA CGACTGCAGA AAGCAGATGT TGTTGCCCAG 
TCAAGGATCT CCTTCCCTGA GAAAGAGCTG CTTTTGTTGA TACGAAACCA TCTTATTTCT 
AAAGGGCTTG GAGAAACAGC AACCGTGCTG ACAAAAGAGG CTGACCTGCC CATGACTGCT 
GCCTCCCATT CTTCTGCCTT TACCCCAGTC ACTGCTGCTG CTTCTCCTGT CTCTCTACCC 
CGAACCCCTC GTATCGCTAA TGGCATTGCA ACTCGTCTGG GCAGCCATGC TGCTGTGGGT 
GCCTCTGCGC CTTCTGCCCC TACTGCTCAT CCTCAGCCAC GGCCCCCCCA GGGTCCGCTA 
GCTCTGCCCG GCCCATCTTA TGCAGGCAAC TCCCCTTTGA TTGGTAGAAT CAGTTTTATC 
AGAGAGAGGC CATCACCCTG CAATGGCAGG AAAATCAGAG TGTTGCGGCA GAAGTCGGAC 
CATGGTGCCT ACAGCCAAAG CCCAGCCATA AAAAAACAGC TGGACAGACA TCTTCCTTCC 
CCACCTACGC TGGACAGTAT AATCACAGAG TATCTTAGAG AACAACATGC TCGCTGCAAG 
AATCCAGTTG CCACCTGCCC ACCTTTCTCC CTCTTTACTC CTCACCAATG TCCTGAGCCA 
AAACAGAGGC GGCAAGCGCC AATAAACTTT ACGTCAAGGC TAAACCGCAG GGCATCATTT 
CCAAAGTATG GAGGGGTGGA TGGCGGATGC TTTGATAGGC ACCTTATCTT TAGCAGATTC 
CGTCCTATTT CAGTGTTCCG GGAAGCCAAT GAAGATGAGA GTGGCTTCAC CTGCTGTGCA 
TTCTCAGCAC GGGAGCGGTT CCTGATGCTT GGCACCTGCA CAGGGCAGCT GAAGCTCTAT 
AATGTGTTTA GTGGACAGGA GGAGGCCAGC TATAACTGTC ACAACTCAGC CATCACACAT 
CTTGAACCTT CCAGGGATGG GTCCTTGCTG CTGACATCTG CTACTTGGAG CCAGCCTTTG 
TCTGCACTTT GGGGAATGAA GTCAGTATTT GATATGAAGC ATTCCTTCAC AGAAGATCAC 
TATGTTGAGT TCAGTAAGCA CTCCCAGGAT CGGGTCATCG GCACAAAAGG AGACATTGCC 
CACATTTATG ATATTCAGAC TGGCAACAAG CTGTTGACTC TGTTTAACCC AGATCTTGCC 
AACAACTACA AGAGGAACTG TGCCACCTTT AATCCTACAG ATGATCTTGT CTTAAATGAT 
GGCGTCCTCT GGGATGTCCG CTCTGCACAG GCCATCCACA AGTTTGACAA GTTCAATATG 
AACATCAGTG GTGTTTTCCA TCCAAATGGA CTGGAGGTGA TCATTAATAC TGAGATTTGG 
GACCTTCGAA CTTTTCATCT TTTGCATACT GTTCCCGCTC TGGATCAGTG TCGCGTGGTG 
TTCAATCACA CGGGAACAGT GATGTATGGA GCTATGTTGC AGGCAGATGA TGAAGATGAC 
TTAATGGAAG AGAGGATGAA AAGCCCCTTT GGGTCATCCT TCCGAACATT TAATGCAACT 
GACTACAAAC CTATAGCAAC CATTGATGTG AAACGGAACA TCTTTGACCT GTGTACAGAC 
ACCAAAGACT GCTATCTTGC TGTCATTGAG AATCAAGGCA GCATGGATGC CCTGAACATG 
GACACAGTAT GCAGGCTGTA TGAAGTGGGC AGGCAGCGTC TGGCAGAGGA TGAGGATGAA 
GAGGAGGACC AGGAAGAGGA AGAACAGGAG GAAGAAGATG ATGATGAAGA TGATGATGAC 
ACCGATGATT TAGATGAGCT TGACACTGAC CAGTTGCTGG AGGCGGAGTT GGAGGAGGAC 
GACAATAATG AGAACGCAGG GGAAGATGGG GACAATGACT TCTCTCCCTC TGATGAGGAG 
CTAGCAAACC TTCTAGAGGA GGGAGAGGAC GGGGAGGATG AAGACTCTGA TGCAGATGAG 
GAGGTGGAAC TGATCCTGGG GGACACTGAC AGCTCTGACA ACTCTGATTT GGAAGATGAC 
ATCATCTTAT CTCTGAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001171904.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001165375.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 2, mRNA.

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CloneID	OHu14003
Clone ID Related Accession (Same CDS sequence)	NM_001171904.1
Accession Version	NM_001171904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR986856.1, BP222559.1, BC110371.1 and AB018343.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR and uses and alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (2) that is 1 aa shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110371.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATCT GGTGAATGCA TATGTGATGA CAAGCCGAGA GCCCCCTTTA 
AACACTGCAG CTTGCAGACT CCTATTAGAC ATCATGCCAG GGCTGGAAAC TGCTGTCGTC 
TTTCAAGAAA AGGAGGGAAT TGTCGAGAAT CTTTTCAAAT GGGCCCGAGA GGCCGATCAA 
CCATTGAGGA CATATTCTAC TGGACTGTTA GGAGGTGCTA TGGAAAATCA AGACATTGCT 
GCCAACTATA GAGATGAAAA TTCACAGCTG GTGGCAATAG TGCTTCGAAG ACTGAGGGAG 
CTACAGCTAC AGGAAGTGGC TTTGCGGCAG GAAAACAAGC GTCCCAGTCC ACGGAAGCTC 
TCTTCTGAAC CCCTTTTGCC TCTGGATGAG GAGGCTGTGG ATATGGACTA TGGTGACATG 
GCTGTAGATG TAGTGGATGG AGACCAAGAG GAAGCTTCTG GAGACATGGA GATCTCCTTT 
CATCTTGATT CAGGCCACAA GACTAGTAGC AGAGTGAACT CAACAACCAA ACCTGAGGAT 
GGAGGATTAA AGAAAAACAA GTCAGCAAAA CAGGGTGACA GAGAGAACTT TAGGAAAGCC 
AAGCAAAAGT TGGGTTTCTC ATCTTCTGAT CCAGATCGCA TGTTTGTTGA GCTGTCTAAT 
AGCAGTTGGT CAGAAATGTC TCCCTGGGTG ATTGGCACCA ATTATACCCT TTATCCTATG 
ACTCCTGCTA TCGAGCAGCG ACTCATTCTC CAATATTTGA CCCCTCTAGG AGAATATCAG 
GAGCTACTTC CCATATTCAT GCAACTTGGA TCACGGGAGC TGATGATGTT CTATATTGAC 
CTGAAGCAAA CTAATGATGT CCTGCTTACA TTTGAGGCAC TAAAGCACCT AGCATCTCTC 
CTGCTCCATA ACAAATTTGC CACAGAATTT GTTGCGCATG GTGGAGTACA GAAATTACTG 
GAAATACCTC GTCCTTCTAT GGCTGCAACC GGTGTGTCTA TGTGTTTGTA TTACCTATCC 
TACAATCAGG ATGCCATGGA AAGAGTTTGC ATGCATCCCC ACAATGTTCT GTCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAGTGT TCTCATGCTT CAGGATGCTG CCATGCTACC 
ATGTTTTTTT CAATTTGCTT CTCATTTCGG GCCGTCTTGG AGCTCTTTGA CCGCTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTCTAAATTT GGAAGATCAG 
GGTGCACTTC TGAGTGATGA TGAAATATTT GCTAGCCGCC AAACTGGGAA ACATACCTGC 
ATGGCCTTGC GCAAATACTT TGAGGCTCAC CTGGCCATTA AATTGGAACA AGTGAAGCAG 
TCACTTCAGA GGACTGAGGG TGGCATTCTT GTCCACCCAC AACCCCCGTA CAAGGCATGC 
TCATATACTC ATGAACAGAT TGTGGAAATG ATGGAATTTT TGATAGAATA TGGCCCAGCG 
CAGCTATATT GGGAACCAGC TGAAGTTTTC CTCAAACTTT CTTGTGTGCA ACTCTTGTTG 
CAGCTTATTT CTATTGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA CACTGTGCGC 
TTTGCTTTGG ATGTCCTGGC TATTCTTACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCA 
GAATCAGTGG ACGTGTTGGA TGAGGCTGGA TCTACAGTCT CTACTGTAGG TATCAGCATT 
ATTTTGGGAG TGGCTGAGGG TGAGTTCTTC ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAGATTA TCATCAATTG TGTGTGTGGC CCAGATAACC GAATATCCAG TATTGGTAAA 
TTTATCTCTG GTACTCCTCG GAGAAAGCTG CCTCAGAACC CTAAAAGCAG TGAGCACACC 
CTGGCCAAGA TGTGGAATGT GGTTCAGTCC AACAACGGCA TCAAGGTGCT CCTGTCCTTA 
CTGTCCATTA AGATGCCCAT CACAGATGCA GACCAAATCC GGGCCCTGGC CTGCAAAGCC 
CTAGTGGGCC TGTCTCGCAG TAGCACTGTC CGGCAGATCA TCAGTAAACT GCCCCTTTTC 
AGCAGCTGCC AGATCCAGCA GCTGATGAAG GAGCCTGTGC TGCAGGACAA GCGCAGTGAC 
CATGTCAAGT TCTGCAAGTA TGCTGCTGAA CTCATTGAAC GGGTGTCAGG AAAACCACTT 
CTCATTGGCA CTGATGTTTC CCTAGCACGA CTGCAGAAAG CAGATGTTGT TGCCCAGTCA 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTT TTGTTGATAC GAAACCATCT TATTTCTAAA 
GGGCTTGGAG AAACAGCAAC CGTGCTGACA AAAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCCCATTCTT CTGCCTTTAC CCCAGTCACT GCTGCTGCTT CTCCTGTCTC TCTACCCCGA 
ACCCCTCGTA TCGCTAATGG CATTGCAACT CGTCTGGGCA GCCATGCTGC TGTGGGTGCC 
TCTGCGCCTT CTGCCCCTAC TGCTCATCCT CAGCCACGGC CCCCCCAGGG TCCGCTAGCT 
CTGCCCGGCC CATCTTATGC AGGCAACTCC CCTTTGATTG GTAGAATCAG TTTTATCAGA 
GAGAGGCCAT CACCCTGCAA TGGCAGGAAA ATCAGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCCTACA GCCAAAGCCC AGCCATAAAA AAACAGCTGG ACAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATAAT CACAGAGTAT CTTAGAGAAC AACATGCTCG CTGCAAGAAT 
CCAGTTGCCA CCTGCCCACC TTTCTCCCTC TTTACTCCTC ACCAATGTCC TGAGCCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACG TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAGTATGGAG GGGTGGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTCCGT 
CCTATTTCAG TGTTCCGGGA AGCCAATGAA GATGAGAGTG GCTTCACCTG CTGTGCATTC 
TCAGCACGGG AGCGGTTCCT GATGCTTGGC ACCTGCACAG GGCAGCTGAA GCTCTATAAT 
GTGTTTAGTG GACAGGAGGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACATCTT 
GAACCTTCCA GGGATGGGTC CTTGCTGCTG ACATCTGCTA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAGTC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC CCAGGATCGG GTCATCGGCA CAAAAGGAGA CATTGCCCAC 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAATATGAAC 
ATCAGTGGTG TTTTCCATCC AAATGGACTG GAGGTGATCA TTAATACTGA GATTTGGGAC 
CTTCGAACTT TTCATCTTTT GCATACTGTT CCCGCTCTGG ATCAGTGTCG CGTGGTGTTC 
AATCACACGG GAACAGTGAT GTATGGAGCT ATGTTGCAGG CAGATGATGA AGATGACTTA 
ATGGAAGAGA GGATGAAAAG CCCCTTTGGG TCATCCTTCC GAACATTTAA TGCAACTGAC 
TACAAACCTA TAGCAACCAT TGATGTGAAA CGGAACATCT TTGACCTGTG TACAGACACC 
AAAGACTGCT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCCCT GAACATGGAC 
ACAGTATGCA GGCTGTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGG AAGAGGAAGA ACAGGAGGAA GAAGATGATG ATGAAGATGA TGATGACACC 
GATGATTTAG ATGAGCTTGA CACTGACCAG TTGCTGGAGG CGGAGTTGGA GGAGGACGAC 
AATAATGAGA ACGCAGGGGA AGATGGGGAC AATGACTTCT CTCCCTCTGA TGAGGAGCTA 
GCAAACCTTC TAGAGGAGGG AGAGGACGGG GAGGATGAAG ACTCTGATGC AGATGAGGAG 
GTGGAACTGA TCCTGGGGGA CACTGACAGC TCTGACAACT CTGATTTGGA AGATGACATC 
ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001165375.1
CDS	139..4659
Translation	MTTVVVHVDSKAELTTLLEQWEKEHGSGQDMVPILTRMSQLIEKETEEYRKGDPDPFDDRHPGRADPECMLGHLLRILFKNDDFMNLVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001171904.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171904.1

ORF Insert Sequence:

ATGACTACAG TAGTGGTACA TGTGGACTCC AAAGCTGAGC TCACTACCCT GCTGGAGCAG 
TGGGAAAAGG AACATGGCAG TGGGCAGGAC ATGGTACCTA TCCTTACCAG GATGTCTCAA 
TTGATTGAAA AAGAAACTGA AGAGTATCGT AAAGGGGATC CAGACCCATT TGATGATCGA 
CATCCTGGTC GAGCTGATCC AGAGTGTATG CTGGGCCACT TGCTGAGAAT ACTCTTCAAG 
AATGATGATT TCATGAATCT GGTGAATGCA TATGTGATGA CAAGCCGAGA GCCCCCTTTA 
AACACTGCAG CTTGCAGACT CCTATTAGAC ATCATGCCAG GGCTGGAAAC TGCTGTCGTC 
TTTCAAGAAA AGGAGGGAAT TGTCGAGAAT CTTTTCAAAT GGGCCCGAGA GGCCGATCAA 
CCATTGAGGA CATATTCTAC TGGACTGTTA GGAGGTGCTA TGGAAAATCA AGACATTGCT 
GCCAACTATA GAGATGAAAA TTCACAGCTG GTGGCAATAG TGCTTCGAAG ACTGAGGGAG 
CTACAGCTAC AGGAAGTGGC TTTGCGGCAG GAAAACAAGC GTCCCAGTCC ACGGAAGCTC 
TCTTCTGAAC CCCTTTTGCC TCTGGATGAG GAGGCTGTGG ATATGGACTA TGGTGACATG 
GCTGTAGATG TAGTGGATGG AGACCAAGAG GAAGCTTCTG GAGACATGGA GATCTCCTTT 
CATCTTGATT CAGGCCACAA GACTAGTAGC AGAGTGAACT CAACAACCAA ACCTGAGGAT 
GGAGGATTAA AGAAAAACAA GTCAGCAAAA CAGGGTGACA GAGAGAACTT TAGGAAAGCC 
AAGCAAAAGT TGGGTTTCTC ATCTTCTGAT CCAGATCGCA TGTTTGTTGA GCTGTCTAAT 
AGCAGTTGGT CAGAAATGTC TCCCTGGGTG ATTGGCACCA ATTATACCCT TTATCCTATG 
ACTCCTGCTA TCGAGCAGCG ACTCATTCTC CAATATTTGA CCCCTCTAGG AGAATATCAG 
GAGCTACTTC CCATATTCAT GCAACTTGGA TCACGGGAGC TGATGATGTT CTATATTGAC 
CTGAAGCAAA CTAATGATGT CCTGCTTACA TTTGAGGCAC TAAAGCACCT AGCATCTCTC 
CTGCTCCATA ACAAATTTGC CACAGAATTT GTTGCGCATG GTGGAGTACA GAAATTACTG 
GAAATACCTC GTCCTTCTAT GGCTGCAACC GGTGTGTCTA TGTGTTTGTA TTACCTATCC 
TACAATCAGG ATGCCATGGA AAGAGTTTGC ATGCATCCCC ACAATGTTCT GTCTGATGTG 
GTGAACTATA CCCTGTGGTT AATGGAGTGT TCTCATGCTT CAGGATGCTG CCATGCTACC 
ATGTTTTTTT CAATTTGCTT CTCATTTCGG GCCGTCTTGG AGCTCTTTGA CCGCTATGAT 
GGTCTTCGTC GTCTGGTGAA CTTGATCAGT ACTTTGGAGA TTCTAAATTT GGAAGATCAG 
GGTGCACTTC TGAGTGATGA TGAAATATTT GCTAGCCGCC AAACTGGGAA ACATACCTGC 
ATGGCCTTGC GCAAATACTT TGAGGCTCAC CTGGCCATTA AATTGGAACA AGTGAAGCAG 
TCACTTCAGA GGACTGAGGG TGGCATTCTT GTCCACCCAC AACCCCCGTA CAAGGCATGC 
TCATATACTC ATGAACAGAT TGTGGAAATG ATGGAATTTT TGATAGAATA TGGCCCAGCG 
CAGCTATATT GGGAACCAGC TGAAGTTTTC CTCAAACTTT CTTGTGTGCA ACTCTTGTTG 
CAGCTTATTT CTATTGCCTG CAATTGGAAG ACCTATTATG CAAGGAATGA CACTGTGCGC 
TTTGCTTTGG ATGTCCTGGC TATTCTTACT GTGGTGCCAA AAATCCAGCT CCAGTTGGCA 
GAATCAGTGG ACGTGTTGGA TGAGGCTGGA TCTACAGTCT CTACTGTAGG TATCAGCATT 
ATTTTGGGAG TGGCTGAGGG TGAGTTCTTC ATCCATGATG CTGAAATTCA GAAGTCAGCA 
CTTCAGATTA TCATCAATTG TGTGTGTGGC CCAGATAACC GAATATCCAG TATTGGTAAA 
TTTATCTCTG GTACTCCTCG GAGAAAGCTG CCTCAGAACC CTAAAAGCAG TGAGCACACC 
CTGGCCAAGA TGTGGAATGT GGTTCAGTCC AACAACGGCA TCAAGGTGCT CCTGTCCTTA 
CTGTCCATTA AGATGCCCAT CACAGATGCA GACCAAATCC GGGCCCTGGC CTGCAAAGCC 
CTAGTGGGCC TGTCTCGCAG TAGCACTGTC CGGCAGATCA TCAGTAAACT GCCCCTTTTC 
AGCAGCTGCC AGATCCAGCA GCTGATGAAG GAGCCTGTGC TGCAGGACAA GCGCAGTGAC 
CATGTCAAGT TCTGCAAGTA TGCTGCTGAA CTCATTGAAC GGGTGTCAGG AAAACCACTT 
CTCATTGGCA CTGATGTTTC CCTAGCACGA CTGCAGAAAG CAGATGTTGT TGCCCAGTCA 
AGGATCTCCT TCCCTGAGAA AGAGCTGCTT TTGTTGATAC GAAACCATCT TATTTCTAAA 
GGGCTTGGAG AAACAGCAAC CGTGCTGACA AAAGAGGCTG ACCTGCCCAT GACTGCTGCC 
TCCCATTCTT CTGCCTTTAC CCCAGTCACT GCTGCTGCTT CTCCTGTCTC TCTACCCCGA 
ACCCCTCGTA TCGCTAATGG CATTGCAACT CGTCTGGGCA GCCATGCTGC TGTGGGTGCC 
TCTGCGCCTT CTGCCCCTAC TGCTCATCCT CAGCCACGGC CCCCCCAGGG TCCGCTAGCT 
CTGCCCGGCC CATCTTATGC AGGCAACTCC CCTTTGATTG GTAGAATCAG TTTTATCAGA 
GAGAGGCCAT CACCCTGCAA TGGCAGGAAA ATCAGAGTGT TGCGGCAGAA GTCGGACCAT 
GGTGCCTACA GCCAAAGCCC AGCCATAAAA AAACAGCTGG ACAGACATCT TCCTTCCCCA 
CCTACGCTGG ACAGTATAAT CACAGAGTAT CTTAGAGAAC AACATGCTCG CTGCAAGAAT 
CCAGTTGCCA CCTGCCCACC TTTCTCCCTC TTTACTCCTC ACCAATGTCC TGAGCCAAAA 
CAGAGGCGGC AAGCGCCAAT AAACTTTACG TCAAGGCTAA ACCGCAGGGC ATCATTTCCA 
AAGTATGGAG GGGTGGATGG CGGATGCTTT GATAGGCACC TTATCTTTAG CAGATTCCGT 
CCTATTTCAG TGTTCCGGGA AGCCAATGAA GATGAGAGTG GCTTCACCTG CTGTGCATTC 
TCAGCACGGG AGCGGTTCCT GATGCTTGGC ACCTGCACAG GGCAGCTGAA GCTCTATAAT 
GTGTTTAGTG GACAGGAGGA GGCCAGCTAT AACTGTCACA ACTCAGCCAT CACACATCTT 
GAACCTTCCA GGGATGGGTC CTTGCTGCTG ACATCTGCTA CTTGGAGCCA GCCTTTGTCT 
GCACTTTGGG GAATGAAGTC AGTATTTGAT ATGAAGCATT CCTTCACAGA AGATCACTAT 
GTTGAGTTCA GTAAGCACTC CCAGGATCGG GTCATCGGCA CAAAAGGAGA CATTGCCCAC 
ATTTATGATA TTCAGACTGG CAACAAGCTG TTGACTCTGT TTAACCCAGA TCTTGCCAAC 
AACTACAAGA GGAACTGTGC CACCTTTAAT CCTACAGATG ATCTTGTCTT AAATGATGGC 
GTCCTCTGGG ATGTCCGCTC TGCACAGGCC ATCCACAAGT TTGACAAGTT CAATATGAAC 
ATCAGTGGTG TTTTCCATCC AAATGGACTG GAGGTGATCA TTAATACTGA GATTTGGGAC 
CTTCGAACTT TTCATCTTTT GCATACTGTT CCCGCTCTGG ATCAGTGTCG CGTGGTGTTC 
AATCACACGG GAACAGTGAT GTATGGAGCT ATGTTGCAGG CAGATGATGA AGATGACTTA 
ATGGAAGAGA GGATGAAAAG CCCCTTTGGG TCATCCTTCC GAACATTTAA TGCAACTGAC 
TACAAACCTA TAGCAACCAT TGATGTGAAA CGGAACATCT TTGACCTGTG TACAGACACC 
AAAGACTGCT ATCTTGCTGT CATTGAGAAT CAAGGCAGCA TGGATGCCCT GAACATGGAC 
ACAGTATGCA GGCTGTATGA AGTGGGCAGG CAGCGTCTGG CAGAGGATGA GGATGAAGAG 
GAGGACCAGG AAGAGGAAGA ACAGGAGGAA GAAGATGATG ATGAAGATGA TGATGACACC 
GATGATTTAG ATGAGCTTGA CACTGACCAG TTGCTGGAGG CGGAGTTGGA GGAGGACGAC 
AATAATGAGA ACGCAGGGGA AGATGGGGAC AATGACTTCT CTCCCTCTGA TGAGGAGCTA 
GCAAACCTTC TAGAGGAGGG AGAGGACGGG GAGGATGAAG ACTCTGATGC AGATGAGGAG 
GTGGAACTGA TCCTGGGGGA CACTGACAGC TCTGACAACT CTGATTTGGA AGATGACATC 
ATCTTATCTC TGAATGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349171.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336100.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 6, mRNA.

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CloneID	OHu37617
Clone ID Related Accession (Same CDS sequence)	NM_001349171.2 , NM_001349171.1
Accession Version	NM_001349171.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Jun 2, 2019 this sequence version replaced NM_001349171.1. Transcript Variant: This variant (6) differs in the 5' and 3' UTR and lacks an alternate coding exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.59421.1, SRR1803617.47807.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGACA TCCTGCTGGT GAATGCATAT GTGATGACAA GCCGAGAGCC CCCTTTAAAC 
ACTGCAGCTT GCAGACTCCT ATTAGACATC ATGCCAGGGC TGGAAACTGC TGTCGTCTTT 
CAAGAAAAGG AGGGAATTGT CGAGAATCTT TTCAAATGGG CCCGAGAGGC CGATCAACCA 
TTGAGGACAT ATTCTACTGG ACTGTTAGGA GGTGCTATGG AAAATCAAGA CATTGCTGCC 
AACTATAGAG ATGAAAATTC ACAGCTGGTG GCAATAGTGC TTCGAAGACT GAGGGAGCTA 
CAGCTACAGG AAGTGGCTTT GCGGCAGGAA AACAAGCGTC CCAGTCCACG GAAGCTCTCT 
TCTGAACCCC TTTTGCCTCT GGATGAGGAG GCTGTGGATA TGGACTATGG TGACATGGCT 
GTAGATGTAG TGGATGGAGA CCAAGAGGAA GCTTCTGGAG ACATGGAGAT CTCCTTTCAT 
CTTGATTCAG GCCACAAGAC TAGTAGCAGA GTGAACTCAA CAACCAAACC TGAGGATGGA 
GGATTAAAGA AAAACAAGTC AGCAAAACAG GGTGACAGAG AGAACTTTAG GAAAGCCAAG 
CAAAAGTTGG GTTTCTCATC TTCTGATCCA GATCGCATGT TTGTTGAGCT GTCTAATAGC 
AGTTGGTCAG AAATGTCTCC CTGGGTGATT GGCACCAATT ATACCCTTTA TCCTATGACT 
CCTGCTATCG AGCAGCGACT CATTCTCCAA TATTTGACCC CTCTAGGAGA ATATCAGGAG 
CTACTTCCCA TATTCATGCA ACTTGGATCA CGGGAGCTGA TGATGTTCTA TATTGACCTG 
AAGCAAACTA ATGATGTCCT GCTTACATTT GAGGCACTAA AGCACCTAGC ATCTCTCCTG 
CTCCATAACA AATTTGCCAC AGAATTTGTT GCGCATGGTG GAGTACAGAA ATTACTGGAA 
ATACCTCGTC CTTCTATGGC TGCAACCGGT GTGTCTATGT GTTTGTATTA CCTATCCTAC 
AATCAGGATG CCATGGAAAG AGTTTGCATG CATCCCCACA ATGTTCTGTC TGATGTGGTG 
AACTATACCC TGTGGTTAAT GGAGTGTTCT CATGCTTCAG GATGCTGCCA TGCTACCATG 
TTTTTTTCAA TTTGCTTCTC ATTTCGGGCC GTCTTGGAGC TCTTTGACCG CTATGATGGT 
CTTCGTCGTC TGGTGAACTT GATCAGTACT TTGGAGATTC TAAATTTGGA AGATCAGGGT 
GCACTTCTGA GTGATGATGA AATATTTGCT AGCCGCCAAA CTGGGAAACA TACCTGCATG 
GCCTTGCGCA AATACTTTGA GGCTCACCTG GCCATTAAAT TGGAACAAGT GAAGCAGTCA 
CTTCAGAGGA CTGAGGGTGG CATTCTTGTC CACCCACAAC CCCCGTACAA GGCATGCTCA 
TATACTCATG AACAGATTGT GGAAATGATG GAATTTTTGA TAGAATATGG CCCAGCGCAG 
CTATATTGGG AACCAGCTGA AGTTTTCCTC AAACTTTCTT GTGTGCAACT CTTGTTGCAG 
CTTATTTCTA TTGCCTGCAA TTGGAAGACC TATTATGCAA GGAATGACAC TGTGCGCTTT 
GCTTTGGATG TCCTGGCTAT TCTTACTGTG GTGCCAAAAA TCCAGCTCCA GTTGGCAGAA 
TCAGTGGACG TGTTGGATGA GGCTGGATCT ACAGTCTCTA CTGTAGGTAT CAGCATTATT 
TTGGGAGTGG CTGAGGGTGA GTTCTTCATC CATGATGCTG AAATTCAGAA GTCAGCACTT 
CAGATTATCA TCAATTGTGT GTGTGGCCCA GATAACCGAA TATCCAGTAT TGGTAAATTT 
ATCTCTGGTA CTCCTCGGAG AAAGCTGCCT CAGAACCCTA AAAGCAGTGA GCACACCCTG 
GCCAAGATGT GGAATGTGGT TCAGTCCAAC AACGGCATCA AGGTGCTCCT GTCCTTACTG 
TCCATTAAGA TGCCCATCAC AGATGCAGAC CAAATCCGGG CCCTGGCCTG CAAAGCCCTA 
GTGGGCCTGT CTCGCAGTAG CACTGTCCGG CAGATCATCA GTAAACTGCC CCTTTTCAGC 
AGCTGCCAGA TCCAGCAGCT GATGAAGGAG CCTGTGCTGC AGGACAAGCG CAGTGACCAT 
GTCAAGTTCT GCAAGTATGC TGCTGAACTC ATTGAACGGG TGTCAGGAAA ACCACTTCTC 
ATTGGCACTG ATGTTTCCCT AGCACGACTG CAGAAAGCAG ATGTTGTTGC CCAGTCAAGG 
ATCTCCTTCC CTGAGAAAGA GCTGCTTTTG TTGATACGAA ACCATCTTAT TTCTAAAGGG 
CTTGGAGAAA CAGCAACCGT GCTGACAAAA GAGGCTGACC TGCCCATGAC TGCTGCCTCC 
CATTCTTCTG CCTTTACCCC AGTCACTGCT GCTGCTTCTC CTGTCTCTCT ACCCCGAACC 
CCTCGTATCG CTAATGGCAT TGCAACTCGT CTGGGCAGCC ATGCTGCTGT GGGTGCCTCT 
GCGCCTTCTG CCCCTACTGC TCATCCTCAG CCACGGCCCC CCCAGGGTCC GCTAGCTCTG 
CCCGGCCCAT CTTATGCAGG CAACTCCCCT TTGATTGGTA GAATCAGTTT TATCAGAGAG 
AGGCCATCAC CCTGCAATGG CAGGAAAATC AGAGTGTTGC GGCAGAAGTC GGACCATGGT 
GCCTACAGCC AAAGCCCAGC CATAAAAAAA CAGCTGGACA GACATCTTCC TTCCCCACCT 
ACGCTGGACA GTATAATCAC AGAGTATCTT AGAGAACAAC ATGCTCGCTG CAAGAATCCA 
GTTGCCACCT GCCCACCTTT CTCCCTCTTT ACTCCTCACC AATGTCCTGA GCCAAAACAG 
AGGCGGCAAG CGCCAATAAA CTTTACGTCA AGGCTAAACC GCAGGGCATC ATTTCCAAAG 
TATGGAGGGG TGGATGGCGG ATGCTTTGAT AGGCACCTTA TCTTTAGCAG ATTCCGTCCT 
ATTTCAGTGT TCCGGGAAGC CAATGAAGAT GAGAGTGGCT TCACCTGCTG TGCATTCTCA 
GCACGGGAGC GGTTCCTGAT GCTTGGCACC TGCACAGGGC AGCTGAAGCT CTATAATGTG 
TTTAGTGGAC AGGAGGAGGC CAGCTATAAC TGTCACAACT CAGCCATCAC ACATCTTGAA 
CCTTCCAGGG ATGGGTCCTT GCTGCTGACA TCTGCTACTT GGAGCCAGCC TTTGTCTGCA 
CTTTGGGGAA TGAAGTCAGT ATTTGATATG AAGCATTCCT TCACAGAAGA TCACTATGTT 
GAGTTCAGTA AGCACTCCCA GGATCGGGTC ATCGGCACAA AAGGAGACAT TGCCCACATT 
TATGATATTC AGACTGGCAA CAAGCTGTTG ACTCTGTTTA ACCCAGATCT TGCCAACAAC 
TACAAGAGGA ACTGTGCCAC CTTTAATCCT ACAGATGATC TTGTCTTAAA TGATGGCGTC 
CTCTGGGATG TCCGCTCTGC ACAGGCCATC CACAAGTTTG ACAAGTTCAA TATGAACATC 
AGTGGTGTTT TCCATCCAAA TGGACTGGAG GTGATCATTA ATACTGAGAT TTGGGACCTT 
CGAACTTTTC ATCTTTTGCA TACTGTTCCC GCTCTGGATC AGTGTCGCGT GGTGTTCAAT 
CACACGGGAA CAGTGATGTA TGGAGCTATG TTGCAGGCAG ATGATGAAGA TGACTTAATG 
GAAGAGAGGA TGAAAAGCCC CTTTGGGTCA TCCTTCCGAA CATTTAATGC AACTGACTAC 
AAACCTATAG CAACCATTGA TGTGAAACGG AACATCTTTG ACCTGTGTAC AGACACCAAA 
GACTGCTATC TTGCTGTCAT TGAGAATCAA GGCAGCATGG ATGCCCTGAA CATGGACACA 
GTATGCAGGC TGTATGAAGT GGGCAGGCAG CGTCTGGCAG AGGATGAGGA TGAAGAGGAG 
GACCAGGAAG AGGAAGAACA GGAGGAAGAA GATGATGATG AAGATGATGA TGACACCGAT 
GATTTAGATG AGCTTGACAC TGACCAGTTG CTGGAGGCGG AGTTGGAGGA GGACGACAAT 
AATGAGAACG CAGGGGAAGA TGGGGACAAT GACTTCTCTC CCTCTGATGA GGAGCTAGCA 
AACCTTCTAG AGGAGGGAGA GGACGGGGAG GATGAAGACT CTGATGCAGA TGAGGAGGTG 
GAACTGATCC TGGGGGACAC TGACAGCTCT GACAACTCTG ATTTGGAAGA TGACATCATC 
TTATCTCTGA ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336100.1
CDS	324..4601
Translation	MIDILLVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349171.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349171.2

ORF Insert Sequence:

ATGATCGACA TCCTGCTGGT GAATGCATAT GTGATGACAA GCCGAGAGCC CCCTTTAAAC 
ACTGCAGCTT GCAGACTCCT ATTAGACATC ATGCCAGGGC TGGAAACTGC TGTCGTCTTT 
CAAGAAAAGG AGGGAATTGT CGAGAATCTT TTCAAATGGG CCCGAGAGGC CGATCAACCA 
TTGAGGACAT ATTCTACTGG ACTGTTAGGA GGTGCTATGG AAAATCAAGA CATTGCTGCC 
AACTATAGAG ATGAAAATTC ACAGCTGGTG GCAATAGTGC TTCGAAGACT GAGGGAGCTA 
CAGCTACAGG AAGTGGCTTT GCGGCAGGAA AACAAGCGTC CCAGTCCACG GAAGCTCTCT 
TCTGAACCCC TTTTGCCTCT GGATGAGGAG GCTGTGGATA TGGACTATGG TGACATGGCT 
GTAGATGTAG TGGATGGAGA CCAAGAGGAA GCTTCTGGAG ACATGGAGAT CTCCTTTCAT 
CTTGATTCAG GCCACAAGAC TAGTAGCAGA GTGAACTCAA CAACCAAACC TGAGGATGGA 
GGATTAAAGA AAAACAAGTC AGCAAAACAG GGTGACAGAG AGAACTTTAG GAAAGCCAAG 
CAAAAGTTGG GTTTCTCATC TTCTGATCCA GATCGCATGT TTGTTGAGCT GTCTAATAGC 
AGTTGGTCAG AAATGTCTCC CTGGGTGATT GGCACCAATT ATACCCTTTA TCCTATGACT 
CCTGCTATCG AGCAGCGACT CATTCTCCAA TATTTGACCC CTCTAGGAGA ATATCAGGAG 
CTACTTCCCA TATTCATGCA ACTTGGATCA CGGGAGCTGA TGATGTTCTA TATTGACCTG 
AAGCAAACTA ATGATGTCCT GCTTACATTT GAGGCACTAA AGCACCTAGC ATCTCTCCTG 
CTCCATAACA AATTTGCCAC AGAATTTGTT GCGCATGGTG GAGTACAGAA ATTACTGGAA 
ATACCTCGTC CTTCTATGGC TGCAACCGGT GTGTCTATGT GTTTGTATTA CCTATCCTAC 
AATCAGGATG CCATGGAAAG AGTTTGCATG CATCCCCACA ATGTTCTGTC TGATGTGGTG 
AACTATACCC TGTGGTTAAT GGAGTGTTCT CATGCTTCAG GATGCTGCCA TGCTACCATG 
TTTTTTTCAA TTTGCTTCTC ATTTCGGGCC GTCTTGGAGC TCTTTGACCG CTATGATGGT 
CTTCGTCGTC TGGTGAACTT GATCAGTACT TTGGAGATTC TAAATTTGGA AGATCAGGGT 
GCACTTCTGA GTGATGATGA AATATTTGCT AGCCGCCAAA CTGGGAAACA TACCTGCATG 
GCCTTGCGCA AATACTTTGA GGCTCACCTG GCCATTAAAT TGGAACAAGT GAAGCAGTCA 
CTTCAGAGGA CTGAGGGTGG CATTCTTGTC CACCCACAAC CCCCGTACAA GGCATGCTCA 
TATACTCATG AACAGATTGT GGAAATGATG GAATTTTTGA TAGAATATGG CCCAGCGCAG 
CTATATTGGG AACCAGCTGA AGTTTTCCTC AAACTTTCTT GTGTGCAACT CTTGTTGCAG 
CTTATTTCTA TTGCCTGCAA TTGGAAGACC TATTATGCAA GGAATGACAC TGTGCGCTTT 
GCTTTGGATG TCCTGGCTAT TCTTACTGTG GTGCCAAAAA TCCAGCTCCA GTTGGCAGAA 
TCAGTGGACG TGTTGGATGA GGCTGGATCT ACAGTCTCTA CTGTAGGTAT CAGCATTATT 
TTGGGAGTGG CTGAGGGTGA GTTCTTCATC CATGATGCTG AAATTCAGAA GTCAGCACTT 
CAGATTATCA TCAATTGTGT GTGTGGCCCA GATAACCGAA TATCCAGTAT TGGTAAATTT 
ATCTCTGGTA CTCCTCGGAG AAAGCTGCCT CAGAACCCTA AAAGCAGTGA GCACACCCTG 
GCCAAGATGT GGAATGTGGT TCAGTCCAAC AACGGCATCA AGGTGCTCCT GTCCTTACTG 
TCCATTAAGA TGCCCATCAC AGATGCAGAC CAAATCCGGG CCCTGGCCTG CAAAGCCCTA 
GTGGGCCTGT CTCGCAGTAG CACTGTCCGG CAGATCATCA GTAAACTGCC CCTTTTCAGC 
AGCTGCCAGA TCCAGCAGCT GATGAAGGAG CCTGTGCTGC AGGACAAGCG CAGTGACCAT 
GTCAAGTTCT GCAAGTATGC TGCTGAACTC ATTGAACGGG TGTCAGGAAA ACCACTTCTC 
ATTGGCACTG ATGTTTCCCT AGCACGACTG CAGAAAGCAG ATGTTGTTGC CCAGTCAAGG 
ATCTCCTTCC CTGAGAAAGA GCTGCTTTTG TTGATACGAA ACCATCTTAT TTCTAAAGGG 
CTTGGAGAAA CAGCAACCGT GCTGACAAAA GAGGCTGACC TGCCCATGAC TGCTGCCTCC 
CATTCTTCTG CCTTTACCCC AGTCACTGCT GCTGCTTCTC CTGTCTCTCT ACCCCGAACC 
CCTCGTATCG CTAATGGCAT TGCAACTCGT CTGGGCAGCC ATGCTGCTGT GGGTGCCTCT 
GCGCCTTCTG CCCCTACTGC TCATCCTCAG CCACGGCCCC CCCAGGGTCC GCTAGCTCTG 
CCCGGCCCAT CTTATGCAGG CAACTCCCCT TTGATTGGTA GAATCAGTTT TATCAGAGAG 
AGGCCATCAC CCTGCAATGG CAGGAAAATC AGAGTGTTGC GGCAGAAGTC GGACCATGGT 
GCCTACAGCC AAAGCCCAGC CATAAAAAAA CAGCTGGACA GACATCTTCC TTCCCCACCT 
ACGCTGGACA GTATAATCAC AGAGTATCTT AGAGAACAAC ATGCTCGCTG CAAGAATCCA 
GTTGCCACCT GCCCACCTTT CTCCCTCTTT ACTCCTCACC AATGTCCTGA GCCAAAACAG 
AGGCGGCAAG CGCCAATAAA CTTTACGTCA AGGCTAAACC GCAGGGCATC ATTTCCAAAG 
TATGGAGGGG TGGATGGCGG ATGCTTTGAT AGGCACCTTA TCTTTAGCAG ATTCCGTCCT 
ATTTCAGTGT TCCGGGAAGC CAATGAAGAT GAGAGTGGCT TCACCTGCTG TGCATTCTCA 
GCACGGGAGC GGTTCCTGAT GCTTGGCACC TGCACAGGGC AGCTGAAGCT CTATAATGTG 
TTTAGTGGAC AGGAGGAGGC CAGCTATAAC TGTCACAACT CAGCCATCAC ACATCTTGAA 
CCTTCCAGGG ATGGGTCCTT GCTGCTGACA TCTGCTACTT GGAGCCAGCC TTTGTCTGCA 
CTTTGGGGAA TGAAGTCAGT ATTTGATATG AAGCATTCCT TCACAGAAGA TCACTATGTT 
GAGTTCAGTA AGCACTCCCA GGATCGGGTC ATCGGCACAA AAGGAGACAT TGCCCACATT 
TATGATATTC AGACTGGCAA CAAGCTGTTG ACTCTGTTTA ACCCAGATCT TGCCAACAAC 
TACAAGAGGA ACTGTGCCAC CTTTAATCCT ACAGATGATC TTGTCTTAAA TGATGGCGTC 
CTCTGGGATG TCCGCTCTGC ACAGGCCATC CACAAGTTTG ACAAGTTCAA TATGAACATC 
AGTGGTGTTT TCCATCCAAA TGGACTGGAG GTGATCATTA ATACTGAGAT TTGGGACCTT 
CGAACTTTTC ATCTTTTGCA TACTGTTCCC GCTCTGGATC AGTGTCGCGT GGTGTTCAAT 
CACACGGGAA CAGTGATGTA TGGAGCTATG TTGCAGGCAG ATGATGAAGA TGACTTAATG 
GAAGAGAGGA TGAAAAGCCC CTTTGGGTCA TCCTTCCGAA CATTTAATGC AACTGACTAC 
AAACCTATAG CAACCATTGA TGTGAAACGG AACATCTTTG ACCTGTGTAC AGACACCAAA 
GACTGCTATC TTGCTGTCAT TGAGAATCAA GGCAGCATGG ATGCCCTGAA CATGGACACA 
GTATGCAGGC TGTATGAAGT GGGCAGGCAG CGTCTGGCAG AGGATGAGGA TGAAGAGGAG 
GACCAGGAAG AGGAAGAACA GGAGGAAGAA GATGATGATG AAGATGATGA TGACACCGAT 
GATTTAGATG AGCTTGACAC TGACCAGTTG CTGGAGGCGG AGTTGGAGGA GGACGACAAT 
AATGAGAACG CAGGGGAAGA TGGGGACAAT GACTTCTCTC CCTCTGATGA GGAGCTAGCA 
AACCTTCTAG AGGAGGGAGA GGACGGGGAG GATGAAGACT CTGATGCAGA TGAGGAGGTG 
GAACTGATCC TGGGGGACAC TGACAGCTCT GACAACTCTG ATTTGGAAGA TGACATCATC 
TTATCTCTGA ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DCAF1 ( NM_001349171.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001336100.1 Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 6, mRNA.

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CloneID	OHu37617
Clone ID Related Accession (Same CDS sequence)	NM_001349171.2 , NM_001349171.1
Accession Version	NM_001349171.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	DDB1- and CUL4-associated factor 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC092037.2 and AC233756.2. On Mar 9, 2017 this sequence version replaced XM_017007551.1. Transcript Variant: This variant (6) differs in the 5' and 3' UTR and lacks an alternate coding exon compared to variant 1. The resulting isoform (3) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.47807.1, SRR1660809.59421.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCGACA TCCTGCTGGT GAATGCATAT GTGATGACAA GCCGAGAGCC CCCTTTAAAC 
ACTGCAGCTT GCAGACTCCT ATTAGACATC ATGCCAGGGC TGGAAACTGC TGTCGTCTTT 
CAAGAAAAGG AGGGAATTGT CGAGAATCTT TTCAAATGGG CCCGAGAGGC CGATCAACCA 
TTGAGGACAT ATTCTACTGG ACTGTTAGGA GGTGCTATGG AAAATCAAGA CATTGCTGCC 
AACTATAGAG ATGAAAATTC ACAGCTGGTG GCAATAGTGC TTCGAAGACT GAGGGAGCTA 
CAGCTACAGG AAGTGGCTTT GCGGCAGGAA AACAAGCGTC CCAGTCCACG GAAGCTCTCT 
TCTGAACCCC TTTTGCCTCT GGATGAGGAG GCTGTGGATA TGGACTATGG TGACATGGCT 
GTAGATGTAG TGGATGGAGA CCAAGAGGAA GCTTCTGGAG ACATGGAGAT CTCCTTTCAT 
CTTGATTCAG GCCACAAGAC TAGTAGCAGA GTGAACTCAA CAACCAAACC TGAGGATGGA 
GGATTAAAGA AAAACAAGTC AGCAAAACAG GGTGACAGAG AGAACTTTAG GAAAGCCAAG 
CAAAAGTTGG GTTTCTCATC TTCTGATCCA GATCGCATGT TTGTTGAGCT GTCTAATAGC 
AGTTGGTCAG AAATGTCTCC CTGGGTGATT GGCACCAATT ATACCCTTTA TCCTATGACT 
CCTGCTATCG AGCAGCGACT CATTCTCCAA TATTTGACCC CTCTAGGAGA ATATCAGGAG 
CTACTTCCCA TATTCATGCA ACTTGGATCA CGGGAGCTGA TGATGTTCTA TATTGACCTG 
AAGCAAACTA ATGATGTCCT GCTTACATTT GAGGCACTAA AGCACCTAGC ATCTCTCCTG 
CTCCATAACA AATTTGCCAC AGAATTTGTT GCGCATGGTG GAGTACAGAA ATTACTGGAA 
ATACCTCGTC CTTCTATGGC TGCAACCGGT GTGTCTATGT GTTTGTATTA CCTATCCTAC 
AATCAGGATG CCATGGAAAG AGTTTGCATG CATCCCCACA ATGTTCTGTC TGATGTGGTG 
AACTATACCC TGTGGTTAAT GGAGTGTTCT CATGCTTCAG GATGCTGCCA TGCTACCATG 
TTTTTTTCAA TTTGCTTCTC ATTTCGGGCC GTCTTGGAGC TCTTTGACCG CTATGATGGT 
CTTCGTCGTC TGGTGAACTT GATCAGTACT TTGGAGATTC TAAATTTGGA AGATCAGGGT 
GCACTTCTGA GTGATGATGA AATATTTGCT AGCCGCCAAA CTGGGAAACA TACCTGCATG 
GCCTTGCGCA AATACTTTGA GGCTCACCTG GCCATTAAAT TGGAACAAGT GAAGCAGTCA 
CTTCAGAGGA CTGAGGGTGG CATTCTTGTC CACCCACAAC CCCCGTACAA GGCATGCTCA 
TATACTCATG AACAGATTGT GGAAATGATG GAATTTTTGA TAGAATATGG CCCAGCGCAG 
CTATATTGGG AACCAGCTGA AGTTTTCCTC AAACTTTCTT GTGTGCAACT CTTGTTGCAG 
CTTATTTCTA TTGCCTGCAA TTGGAAGACC TATTATGCAA GGAATGACAC TGTGCGCTTT 
GCTTTGGATG TCCTGGCTAT TCTTACTGTG GTGCCAAAAA TCCAGCTCCA GTTGGCAGAA 
TCAGTGGACG TGTTGGATGA GGCTGGATCT ACAGTCTCTA CTGTAGGTAT CAGCATTATT 
TTGGGAGTGG CTGAGGGTGA GTTCTTCATC CATGATGCTG AAATTCAGAA GTCAGCACTT 
CAGATTATCA TCAATTGTGT GTGTGGCCCA GATAACCGAA TATCCAGTAT TGGTAAATTT 
ATCTCTGGTA CTCCTCGGAG AAAGCTGCCT CAGAACCCTA AAAGCAGTGA GCACACCCTG 
GCCAAGATGT GGAATGTGGT TCAGTCCAAC AACGGCATCA AGGTGCTCCT GTCCTTACTG 
TCCATTAAGA TGCCCATCAC AGATGCAGAC CAAATCCGGG CCCTGGCCTG CAAAGCCCTA 
GTGGGCCTGT CTCGCAGTAG CACTGTCCGG CAGATCATCA GTAAACTGCC CCTTTTCAGC 
AGCTGCCAGA TCCAGCAGCT GATGAAGGAG CCTGTGCTGC AGGACAAGCG CAGTGACCAT 
GTCAAGTTCT GCAAGTATGC TGCTGAACTC ATTGAACGGG TGTCAGGAAA ACCACTTCTC 
ATTGGCACTG ATGTTTCCCT AGCACGACTG CAGAAAGCAG ATGTTGTTGC CCAGTCAAGG 
ATCTCCTTCC CTGAGAAAGA GCTGCTTTTG TTGATACGAA ACCATCTTAT TTCTAAAGGG 
CTTGGAGAAA CAGCAACCGT GCTGACAAAA GAGGCTGACC TGCCCATGAC TGCTGCCTCC 
CATTCTTCTG CCTTTACCCC AGTCACTGCT GCTGCTTCTC CTGTCTCTCT ACCCCGAACC 
CCTCGTATCG CTAATGGCAT TGCAACTCGT CTGGGCAGCC ATGCTGCTGT GGGTGCCTCT 
GCGCCTTCTG CCCCTACTGC TCATCCTCAG CCACGGCCCC CCCAGGGTCC GCTAGCTCTG 
CCCGGCCCAT CTTATGCAGG CAACTCCCCT TTGATTGGTA GAATCAGTTT TATCAGAGAG 
AGGCCATCAC CCTGCAATGG CAGGAAAATC AGAGTGTTGC GGCAGAAGTC GGACCATGGT 
GCCTACAGCC AAAGCCCAGC CATAAAAAAA CAGCTGGACA GACATCTTCC TTCCCCACCT 
ACGCTGGACA GTATAATCAC AGAGTATCTT AGAGAACAAC ATGCTCGCTG CAAGAATCCA 
GTTGCCACCT GCCCACCTTT CTCCCTCTTT ACTCCTCACC AATGTCCTGA GCCAAAACAG 
AGGCGGCAAG CGCCAATAAA CTTTACGTCA AGGCTAAACC GCAGGGCATC ATTTCCAAAG 
TATGGAGGGG TGGATGGCGG ATGCTTTGAT AGGCACCTTA TCTTTAGCAG ATTCCGTCCT 
ATTTCAGTGT TCCGGGAAGC CAATGAAGAT GAGAGTGGCT TCACCTGCTG TGCATTCTCA 
GCACGGGAGC GGTTCCTGAT GCTTGGCACC TGCACAGGGC AGCTGAAGCT CTATAATGTG 
TTTAGTGGAC AGGAGGAGGC CAGCTATAAC TGTCACAACT CAGCCATCAC ACATCTTGAA 
CCTTCCAGGG ATGGGTCCTT GCTGCTGACA TCTGCTACTT GGAGCCAGCC TTTGTCTGCA 
CTTTGGGGAA TGAAGTCAGT ATTTGATATG AAGCATTCCT TCACAGAAGA TCACTATGTT 
GAGTTCAGTA AGCACTCCCA GGATCGGGTC ATCGGCACAA AAGGAGACAT TGCCCACATT 
TATGATATTC AGACTGGCAA CAAGCTGTTG ACTCTGTTTA ACCCAGATCT TGCCAACAAC 
TACAAGAGGA ACTGTGCCAC CTTTAATCCT ACAGATGATC TTGTCTTAAA TGATGGCGTC 
CTCTGGGATG TCCGCTCTGC ACAGGCCATC CACAAGTTTG ACAAGTTCAA TATGAACATC 
AGTGGTGTTT TCCATCCAAA TGGACTGGAG GTGATCATTA ATACTGAGAT TTGGGACCTT 
CGAACTTTTC ATCTTTTGCA TACTGTTCCC GCTCTGGATC AGTGTCGCGT GGTGTTCAAT 
CACACGGGAA CAGTGATGTA TGGAGCTATG TTGCAGGCAG ATGATGAAGA TGACTTAATG 
GAAGAGAGGA TGAAAAGCCC CTTTGGGTCA TCCTTCCGAA CATTTAATGC AACTGACTAC 
AAACCTATAG CAACCATTGA TGTGAAACGG AACATCTTTG ACCTGTGTAC AGACACCAAA 
GACTGCTATC TTGCTGTCAT TGAGAATCAA GGCAGCATGG ATGCCCTGAA CATGGACACA 
GTATGCAGGC TGTATGAAGT GGGCAGGCAG CGTCTGGCAG AGGATGAGGA TGAAGAGGAG 
GACCAGGAAG AGGAAGAACA GGAGGAAGAA GATGATGATG AAGATGATGA TGACACCGAT 
GATTTAGATG AGCTTGACAC TGACCAGTTG CTGGAGGCGG AGTTGGAGGA GGACGACAAT 
AATGAGAACG CAGGGGAAGA TGGGGACAAT GACTTCTCTC CCTCTGATGA GGAGCTAGCA 
AACCTTCTAG AGGAGGGAGA GGACGGGGAG GATGAAGACT CTGATGCAGA TGAGGAGGTG 
GAACTGATCC TGGGGGACAC TGACAGCTCT GACAACTCTG ATTTGGAAGA TGACATCATC 
TTATCTCTGA ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001336100.1
CDS	311..4588
Translation	MIDILLVNAYVMTSREPPLNTAACRLLLDIMPGLETAVVFQEKEGIVENLFKWAREADQPLRTYSTGLLGGAMENQDIAANYRDENSQLVAIVLRRLRELQLQEVALRQENKRPSPRKLSSEPLLPLDEEAVDMDYGDMAVDVVDGDQEEASGDMEISFHLDSGHKTSSRVNSTTKPEDGGLKKNKSAKQGDRENFRKAKQKLGFSSSDPDRMFVELSNSSWSEMSPWVIGTNYTLYPMTPAIEQRLILQYLTPLGEYQELLPIFMQLGSRELMMFYIDLKQTNDVLLTFEALKHLASLLLHNKFATEFVAHGGVQKLLEIPRPSMAATGVSMCLYYLSYNQDAMERVCMHPHNVLSDVVNYTLWLMECSHASGCCHATMFFSICFSFRAVLELFDRYDGLRRLVNLISTLEILNLEDQGALLSDDEIFASRQTGKHTCMALRKYFEAHLAIKLEQVKQSLQRTEGGILVHPQPPYKACSYTHEQIVEMMEFLIEYGPAQLYWEPAEVFLKLSCVQLLLQLISIACNWKTYYARNDTVRFALDVLAILTVVPKIQLQLAESVDVLDEAGSTVSTVGISIILGVAEGEFFIHDAEIQKSALQIIINCVCGPDNRISSIGKFISGTPRRKLPQNPKSSEHTLAKMWNVVQSNNGIKVLLSLLSIKMPITDADQIRALACKALVGLSRSSTVRQIISKLPLFSSCQIQQLMKEPVLQDKRSDHVKFCKYAAELIERVSGKPLLIGTDVSLARLQKADVVAQSRISFPEKELLLLIRNHLISKGLGETATVLTKEADLPMTAASHSSAFTPVTAAASPVSLPRTPRIANGIATRLGSHAAVGASAPSAPTAHPQPRPPQGPLALPGPSYAGNSPLIGRISFIRERPSPCNGRKIRVLRQKSDHGAYSQSPAIKKQLDRHLPSPPTLDSIITEYLREQHARCKNPVATCPPFSLFTPHQCPEPKQRRQAPINFTSRLNRRASFPKYGGVDGGCFDRHLIFSRFRPISVFREANEDESGFTCCAFSARERFLMLGTCTGQLKLYNVFSGQEEASYNCHNSAITHLEPSRDGSLLLTSATWSQPLSALWGMKSVFDMKHSFTEDHYVEFSKHSQDRVIGTKGDIAHIYDIQTGNKLLTLFNPDLANNYKRNCATFNPTDDLVLNDGVLWDVRSAQAIHKFDKFNMNISGVFHPNGLEVIINTEIWDLRTFHLLHTVPALDQCRVVFNHTGTVMYGAMLQADDEDDLMEERMKSPFGSSFRTFNATDYKPIATIDVKRNIFDLCTDTKDCYLAVIENQGSMDALNMDTVCRLYEVGRQRLAEDEDEEEDQEEEEQEEEDDDEDDDDTDDLDELDTDQLLEAELEEDDNNENAGEDGDNDFSPSDEELANLLEEGEDGEDEDSDADEEVELILGDTDSSDNSDLEDDIILSLNE

Target ORF information:

RefSeq Version	NM_001349171.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DDB1 and CUL4 associated factor 1 (DCAF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001349171.1

ORF Insert Sequence:

ATGATCGACA TCCTGCTGGT GAATGCATAT GTGATGACAA GCCGAGAGCC CCCTTTAAAC 
ACTGCAGCTT GCAGACTCCT ATTAGACATC ATGCCAGGGC TGGAAACTGC TGTCGTCTTT 
CAAGAAAAGG AGGGAATTGT CGAGAATCTT TTCAAATGGG CCCGAGAGGC CGATCAACCA 
TTGAGGACAT ATTCTACTGG ACTGTTAGGA GGTGCTATGG AAAATCAAGA CATTGCTGCC 
AACTATAGAG ATGAAAATTC ACAGCTGGTG GCAATAGTGC TTCGAAGACT GAGGGAGCTA 
CAGCTACAGG AAGTGGCTTT GCGGCAGGAA AACAAGCGTC CCAGTCCACG GAAGCTCTCT 
TCTGAACCCC TTTTGCCTCT GGATGAGGAG GCTGTGGATA TGGACTATGG TGACATGGCT 
GTAGATGTAG TGGATGGAGA CCAAGAGGAA GCTTCTGGAG ACATGGAGAT CTCCTTTCAT 
CTTGATTCAG GCCACAAGAC TAGTAGCAGA GTGAACTCAA CAACCAAACC TGAGGATGGA 
GGATTAAAGA AAAACAAGTC AGCAAAACAG GGTGACAGAG AGAACTTTAG GAAAGCCAAG 
CAAAAGTTGG GTTTCTCATC TTCTGATCCA GATCGCATGT TTGTTGAGCT GTCTAATAGC 
AGTTGGTCAG AAATGTCTCC CTGGGTGATT GGCACCAATT ATACCCTTTA TCCTATGACT 
CCTGCTATCG AGCAGCGACT CATTCTCCAA TATTTGACCC CTCTAGGAGA ATATCAGGAG 
CTACTTCCCA TATTCATGCA ACTTGGATCA CGGGAGCTGA TGATGTTCTA TATTGACCTG 
AAGCAAACTA ATGATGTCCT GCTTACATTT GAGGCACTAA AGCACCTAGC ATCTCTCCTG 
CTCCATAACA AATTTGCCAC AGAATTTGTT GCGCATGGTG GAGTACAGAA ATTACTGGAA 
ATACCTCGTC CTTCTATGGC TGCAACCGGT GTGTCTATGT GTTTGTATTA CCTATCCTAC 
AATCAGGATG CCATGGAAAG AGTTTGCATG CATCCCCACA ATGTTCTGTC TGATGTGGTG 
AACTATACCC TGTGGTTAAT GGAGTGTTCT CATGCTTCAG GATGCTGCCA TGCTACCATG 
TTTTTTTCAA TTTGCTTCTC ATTTCGGGCC GTCTTGGAGC TCTTTGACCG CTATGATGGT 
CTTCGTCGTC TGGTGAACTT GATCAGTACT TTGGAGATTC TAAATTTGGA AGATCAGGGT 
GCACTTCTGA GTGATGATGA AATATTTGCT AGCCGCCAAA CTGGGAAACA TACCTGCATG 
GCCTTGCGCA AATACTTTGA GGCTCACCTG GCCATTAAAT TGGAACAAGT GAAGCAGTCA 
CTTCAGAGGA CTGAGGGTGG CATTCTTGTC CACCCACAAC CCCCGTACAA GGCATGCTCA 
TATACTCATG AACAGATTGT GGAAATGATG GAATTTTTGA TAGAATATGG CCCAGCGCAG 
CTATATTGGG AACCAGCTGA AGTTTTCCTC AAACTTTCTT GTGTGCAACT CTTGTTGCAG 
CTTATTTCTA TTGCCTGCAA TTGGAAGACC TATTATGCAA GGAATGACAC TGTGCGCTTT 
GCTTTGGATG TCCTGGCTAT TCTTACTGTG GTGCCAAAAA TCCAGCTCCA GTTGGCAGAA 
TCAGTGGACG TGTTGGATGA GGCTGGATCT ACAGTCTCTA CTGTAGGTAT CAGCATTATT 
TTGGGAGTGG CTGAGGGTGA GTTCTTCATC CATGATGCTG AAATTCAGAA GTCAGCACTT 
CAGATTATCA TCAATTGTGT GTGTGGCCCA GATAACCGAA TATCCAGTAT TGGTAAATTT 
ATCTCTGGTA CTCCTCGGAG AAAGCTGCCT CAGAACCCTA AAAGCAGTGA GCACACCCTG 
GCCAAGATGT GGAATGTGGT TCAGTCCAAC AACGGCATCA AGGTGCTCCT GTCCTTACTG 
TCCATTAAGA TGCCCATCAC AGATGCAGAC CAAATCCGGG CCCTGGCCTG CAAAGCCCTA 
GTGGGCCTGT CTCGCAGTAG CACTGTCCGG CAGATCATCA GTAAACTGCC CCTTTTCAGC 
AGCTGCCAGA TCCAGCAGCT GATGAAGGAG CCTGTGCTGC AGGACAAGCG CAGTGACCAT 
GTCAAGTTCT GCAAGTATGC TGCTGAACTC ATTGAACGGG TGTCAGGAAA ACCACTTCTC 
ATTGGCACTG ATGTTTCCCT AGCACGACTG CAGAAAGCAG ATGTTGTTGC CCAGTCAAGG 
ATCTCCTTCC CTGAGAAAGA GCTGCTTTTG TTGATACGAA ACCATCTTAT TTCTAAAGGG 
CTTGGAGAAA CAGCAACCGT GCTGACAAAA GAGGCTGACC TGCCCATGAC TGCTGCCTCC 
CATTCTTCTG CCTTTACCCC AGTCACTGCT GCTGCTTCTC CTGTCTCTCT ACCCCGAACC 
CCTCGTATCG CTAATGGCAT TGCAACTCGT CTGGGCAGCC ATGCTGCTGT GGGTGCCTCT 
GCGCCTTCTG CCCCTACTGC TCATCCTCAG CCACGGCCCC CCCAGGGTCC GCTAGCTCTG 
CCCGGCCCAT CTTATGCAGG CAACTCCCCT TTGATTGGTA GAATCAGTTT TATCAGAGAG 
AGGCCATCAC CCTGCAATGG CAGGAAAATC AGAGTGTTGC GGCAGAAGTC GGACCATGGT 
GCCTACAGCC AAAGCCCAGC CATAAAAAAA CAGCTGGACA GACATCTTCC TTCCCCACCT 
ACGCTGGACA GTATAATCAC AGAGTATCTT AGAGAACAAC ATGCTCGCTG CAAGAATCCA 
GTTGCCACCT GCCCACCTTT CTCCCTCTTT ACTCCTCACC AATGTCCTGA GCCAAAACAG 
AGGCGGCAAG CGCCAATAAA CTTTACGTCA AGGCTAAACC GCAGGGCATC ATTTCCAAAG 
TATGGAGGGG TGGATGGCGG ATGCTTTGAT AGGCACCTTA TCTTTAGCAG ATTCCGTCCT 
ATTTCAGTGT TCCGGGAAGC CAATGAAGAT GAGAGTGGCT TCACCTGCTG TGCATTCTCA 
GCACGGGAGC GGTTCCTGAT GCTTGGCACC TGCACAGGGC AGCTGAAGCT CTATAATGTG 
TTTAGTGGAC AGGAGGAGGC CAGCTATAAC TGTCACAACT CAGCCATCAC ACATCTTGAA 
CCTTCCAGGG ATGGGTCCTT GCTGCTGACA TCTGCTACTT GGAGCCAGCC TTTGTCTGCA 
CTTTGGGGAA TGAAGTCAGT ATTTGATATG AAGCATTCCT TCACAGAAGA TCACTATGTT 
GAGTTCAGTA AGCACTCCCA GGATCGGGTC ATCGGCACAA AAGGAGACAT TGCCCACATT 
TATGATATTC AGACTGGCAA CAAGCTGTTG ACTCTGTTTA ACCCAGATCT TGCCAACAAC 
TACAAGAGGA ACTGTGCCAC CTTTAATCCT ACAGATGATC TTGTCTTAAA TGATGGCGTC 
CTCTGGGATG TCCGCTCTGC ACAGGCCATC CACAAGTTTG ACAAGTTCAA TATGAACATC 
AGTGGTGTTT TCCATCCAAA TGGACTGGAG GTGATCATTA ATACTGAGAT TTGGGACCTT 
CGAACTTTTC ATCTTTTGCA TACTGTTCCC GCTCTGGATC AGTGTCGCGT GGTGTTCAAT 
CACACGGGAA CAGTGATGTA TGGAGCTATG TTGCAGGCAG ATGATGAAGA TGACTTAATG 
GAAGAGAGGA TGAAAAGCCC CTTTGGGTCA TCCTTCCGAA CATTTAATGC AACTGACTAC 
AAACCTATAG CAACCATTGA TGTGAAACGG AACATCTTTG ACCTGTGTAC AGACACCAAA 
GACTGCTATC TTGCTGTCAT TGAGAATCAA GGCAGCATGG ATGCCCTGAA CATGGACACA 
GTATGCAGGC TGTATGAAGT GGGCAGGCAG CGTCTGGCAG AGGATGAGGA TGAAGAGGAG 
GACCAGGAAG AGGAAGAACA GGAGGAAGAA GATGATGATG AAGATGATGA TGACACCGAT 
GATTTAGATG AGCTTGACAC TGACCAGTTG CTGGAGGCGG AGTTGGAGGA GGACGACAAT 
AATGAGAACG CAGGGGAAGA TGGGGACAAT GACTTCTCTC CCTCTGATGA GGAGCTAGCA 
AACCTTCTAG AGGAGGGAGA GGACGGGGAG GATGAAGACT CTGATGCAGA TGAGGAGGTG 
GAACTGATCC TGGGGGACAC TGACAGCTCT GACAACTCTG ATTTGGAAGA TGACATCATC 
TTATCTCTGA ATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DCAF1 is involved in HCV replication through regulation of miR-122.
Archives of virology(2018 Jan)
Yan Y,Li C,Sun B,Yang R

HIV-1 Vpr protein directly loads helicase-like transcription factor (HLTF) onto the CRL4-DCAF1 E3 ubiquitin ligase.
The Journal of biological chemistry292(51)21117-21127(2017 Dec)
Zhou X,DeLucia M,Hao C,Hrecka K,Monnie C,Skowronski J,Ahn J

VprBP/DCAF1 Regulates the Degradation and Nonproteolytic Activation of the Cell Cycle Transcription Factor FoxM1.
Molecular and cellular biology37(13)(2017 Jul)
Wang X,Arceci A,Bird K,Mills CA,Choudhury R,Kernan JL,Zhou C,Bae-Jump V,Bowers A,Emanuele MJ

Virion-Associated Vpr Alleviates a Postintegration Block to HIV-1 Infection of Dendritic Cells.
Journal of virology91(13)(2017 Jul)
Miller CM,Akiyama H,Agosto LM,Emery A,Ettinger CR,Swanstrom RI,Henderson AJ,Gummuluru S

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DCAF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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