SEMA3E cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA3E
Entrez Gene ID	9723
Full Name	semaphorin 3E
Synonyms	M-SEMAH,M-SemaK,SEMAH,coll-5
General protein information	Preferred Names semaphorin 3E Names semaphorin-3E sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.11
Summary	Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010].
Disorder MIM:	608166
Disorder Html:	?CHARGE syndrome, 214800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_012431.2	NP_036563.1	semaphorin-3E isoform 1 precursor
NM_012431.3	NP_036563.1	semaphorin-3E isoform 1 precursor
NM_001178129.1	NP_001171600.1	semaphorin-3E isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	SEMA3E	NP_036563.1
Pan troglodytes (chimpanzee)	SEMA3E	XP_527803.3
Mus musculus (house mouse)	Sema3e	NP_035478.2
Rattus norvegicus (Norway rat)	Sema3e	NP_001100049.1
	SEMA3E	XP_002803437.1
Gallus gallus (chicken)	SEMA3E	NP_989573.1
Danio rerio (zebrafish)	sema3e	NP_001038549.1
Canis lupus familiaris (dog)	SEMA3E	XP_005631079.1
Bos taurus (cattle)	SEMA3E	XP_003586003.2
Xenopus tropicalis (tropical clawed frog)	sema3e	XP_002935302.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of semaphorin 3E is suppressed in severe asthma.
Movassagh H, Shan L, Chakir J, McConville JF, Halayko AJ, Koussih L, Gounni AS
The Journal of allergy and clinical immunology140(4)1176-1179(2017 Oct)

Chemorepellent Semaphorin 3E Negatively Regulates Neutrophil Migration In Vitro and In Vivo.
Movassagh H, Saati A, Nandagopal S, Mohammed A, Tatari N, Shan L, Duke-Cohan JS, Fowke KR, Lin F, Gounni AS
Journal of immunology (Baltimore, Md. : 1950)198(3)1023-1033(2017 Feb)

Association of Increased Serum Sema3E with TRIB3 Q84R Polymorphism and Carotid Atherosclerosis in Metabolic Syndrome.
Qin RR, Song M, Li YH, Wang F, Zhou HM, Liu MH, Zhong M, Zhang Y, Zhang W, Wang ZH
Annals of clinical and laboratory science47(1)47-51(2017 Jan)

Overexpression of Semaphorin-3E enhances pancreatic cancer cell growth and associates with poor patient survival.
Yong LK, Lai S, Liang Z, Poteet E, Chen F, van Buren G, Fisher W, Mo Q, Chen C, Yao Q
Oncotarget7(52)87431-87448(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Sema3E could be considered an essential regulatory mediator involved in modulation of neutrophil migration throughout the course of neutrophilic inflammation.
Title: Chemorepellent Semaphorin 3E Negatively Regulates Neutrophil Migration In Vitro and In Vivo.

This study aims to investigate whether the Tribbles 3 Q84R polymorphism has profound effects on serum semaphorin 3E and what effect semaphorin 3E exerts on carotid atherosclerosis.
Title: Association of Increased Serum Sema3E with TRIB3 Q84R Polymorphism and Carotid Atherosclerosis in Metabolic Syndrome.

This study suggests that NRP1 expression and LVD are independent factors that are likely to predict the risk of LN metastasis in squamous cell carcinoma (SCC)of the tongue, whereas the expression of VEGFC, VEGFR3, CCR7, and SEMA3E are nonindependent predictive factors
Title: Use of NRP1, a novel biomarker, along with VEGF-C, VEGFR-3, CCR7 and SEMA3E, to predict lymph node metastasis in squamous cell carcinoma of the tongue.

Results show high expression level of SEMA3E which correlated with lymph node involvement, metastatic progression and a significant association with poor prognosis in gastric cancer patients. Also, in vitro studies reveal that SEMA3E caused cell proliferation and anchorage-independent cell growth in the intestinal type of gastric cancer.
Title: Enhanced expression of semaphorin 3E is involved in the gastric cancer development.

suggested that miR-4282 is a tumor suppressor in colorectal carcinoma cells and exerted its inhibitory effect on the tumor cells through targeting Sema3E by inhibiting Sema3E translation or enhancing Sema3E mRNA degradation
Title: MiR-4282 suppresses proliferation and mobility of human colorectal carcinoma cells by targeting semaphorin 3E.

The following SEMA3E gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA3E cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12649	NM_001178129.1 Latest version!	Homo sapiens semaphorin 3E (SEMA3E), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu22789	NM_012431.3 Latest version!	Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu22789	NM_012431.2	Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA3E ( NM_001178129.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171600.1 Homo sapiens semaphorin 3E (SEMA3E), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12649
Clone ID Related Accession (Same CDS sequence)	NM_001178129.1
Accession Version	NM_001178129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	semaphorin-3E isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303925.1, BC152458.1 and AC079799.7. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303925.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2467144 [ECO:0000350] ##Evidence-Data-END##

ATGCTGCTGG ATGAATATCA AGAGAGGCTC TTCGTGGGAG GCAGGGACCT TGTATATTCC 
CTCAGCTTGG AGAGAATCAG TGACGGCTAT AAAGAGATAC ACTGGCCGAG TACAGCTCTA 
AAAATGGAAG AATGCATAAT GAAGGGAAAA GATGCGGGTG AATGTGCAAA TTATGTTCGG 
GTTTTGCATC ACTATAACAG GACACACCTT CTGACCTGTG GTACTGGAGC TTTTGATCCA 
GTTTGTGCCT TCATCAGAGT TGGATATCAT TTGGAGGATC CTCTGTTTCA CCTGGAATCA 
CCCAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAGCTCCTC CTTCATCTCC 
ACTTTAATTG GTAGTGAATT GTTTGCTGGA CTCTACAGTG ACTACTGGAG CAGAGACGCT 
GCGATCTTCC GCAGCATGGG GCGACTGGCC CATATCCGCA CTGAGCATGA CGATGAGCGT 
CTGTTGAAAG AACCAAAATT TGTAGGTTCA TACATGATTC CTGACAATGA AGACAGAGAT 
GACAACAAAG TATATTTCTT TTTTACTGAG AAGGCACTGG AGGCAGAAAA CAATGCTCAC 
GCAATTTACA CCAGGGTCGG GCGACTCTGT GTGAATGATG TAGGAGGGCA GAGAATACTG 
GTGAATAAGT GGAGCACTTT CCTAAAAGCG AGACTCGTTT GCTCAGTACC AGGAATGAAT 
GGAATTGACA CATATTTTGA TGAATTAGAG GACGTTTTTT TGCTACCTAC CAGAGATCAT 
AAGAATCCAG TGATATTTGG ACTCTTTAAC ACTACCAGTA ATATTTTTCG AGGGCATGCT 
ATATGTGTCT ATCACATGTC TAGCATTCGG GCAGCCTTCA ACGGACCATA TGCACATAAG 
GAAGGACCTG AATACCACTG GTCAGTCTAT GAAGGAAAAG TCCCTTATCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTAAA TGGAGGGAGA TACGGAACCA CCAAGGACTA TCCTGATGAT 
GCCATCCGAT TTGCAAGAAG TCATCCACTA ATGTACCAGG CCATAAAACC TGCCCATAAA 
AAACCAATAT TGGTAAAAAC AGATGGAAAA TATAACCTGA AACAAATAGC AGTAGATCGA 
GTGGAAGCTG AGGATGGCCA ATATGACGTC TTGTTTATTG GGACAGATAA TGGAATTGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ATGGAATCAA TGGAAGAAGT AATTCTAGAA 
GAACTTCAGA TATTCAAGGA TCCAGTTCCT ATTATTTCTA TGGAGATTTC TTCAAAGCGG 
CAACAGCTGT ATATTGGATC TGCTTCTGCT GTGGCTCAAG TCAGATTCCA TCACTGTGAC 
ATGTATGGAA GTGCTTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGCATATCCT GCTCCCGGTA TTACCCAACA GGCACACATG CAAAAAGGCG TTTCCGGAGA 
CAAGATGTTC GACATGGAAA TGCAGCTCAG CAGTGCTTTG GACAACAGTT TGTTGGGGAT 
GCTTTGGATA AGACTGAAGA ACATCTGGCT TATGGCATAG AGAACAACAG TACTTTGCTG 
GAATGTACCC CACGATCTTT ACAAGCGAAA GTTATCTGGT TTGTACAGAA AGGACGTGAG 
ACAAGAAAAG AGGAGGTGAA GACAGATGAC AGAGTGGTTA AGATGGACCT TGGTTTACTC 
TTCCTAAGGT TACACAAATC AGATGCTGGG ACCTATTTTT GCCAGACAGT AGAGCATAGC 
TTTGTCCATA CGGTCCGTAA AATCACCTTG GAGGTAGTGG AAGAGGAGAA AGTCGAGGAT 
ATGTTTAACA AGGACGATGA GGAGGACAGG CATCACAGGA TGCCTTGTCC TGCTCAGAGT 
AGCATCTCGC AGGGAGCAAA ACCATGGTAC AAGGAATTCT TGCAGCTGAT CGGTTATAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAG AAAGTATGGT GCACAGATAG AAAGAGGAAA 
AAGCTTAAAA TGTCACCCTC CAAGTGGAAG TATGCCAACC CTCAGGAAAA GAAGCTCCGT 
TCCAAACCTG AGCATTACCG CCTGCCCAGG CACACGCTGG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171600.1
CDS	166..2313
Translation	MLLDEYQERLFVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHLLTCGTGAFDPVCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDAAIFRSMGRLAHIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLCVNDVGGQRILVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHAICVYHMSSIRAAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPLMYQAIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILEELQIFKDPVPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGTHAKRRFRRQDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRETRKEEVKTDDRVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDRHHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLRSKPEHYRLPRHTLDS

Target ORF information:

RefSeq Version	NM_001178129.1
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3E (SEMA3E), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001178129.1

ORF Insert Sequence:

ATGCTGCTGG ATGAATATCA AGAGAGGCTC TTCGTGGGAG GCAGGGACCT TGTATATTCC 
CTCAGCTTGG AGAGAATCAG TGACGGCTAT AAAGAGATAC ACTGGCCGAG TACAGCTCTA 
AAAATGGAAG AATGCATAAT GAAGGGAAAA GATGCGGGTG AATGTGCAAA TTATGTTCGG 
GTTTTGCATC ACTATAACAG GACACACCTT CTGACCTGTG GTACTGGAGC TTTTGATCCA 
GTTTGTGCCT TCATCAGAGT TGGATATCAT TTGGAGGATC CTCTGTTTCA CCTGGAATCA 
CCCAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAGCTCCTC CTTCATCTCC 
ACTTTAATTG GTAGTGAATT GTTTGCTGGA CTCTACAGTG ACTACTGGAG CAGAGACGCT 
GCGATCTTCC GCAGCATGGG GCGACTGGCC CATATCCGCA CTGAGCATGA CGATGAGCGT 
CTGTTGAAAG AACCAAAATT TGTAGGTTCA TACATGATTC CTGACAATGA AGACAGAGAT 
GACAACAAAG TATATTTCTT TTTTACTGAG AAGGCACTGG AGGCAGAAAA CAATGCTCAC 
GCAATTTACA CCAGGGTCGG GCGACTCTGT GTGAATGATG TAGGAGGGCA GAGAATACTG 
GTGAATAAGT GGAGCACTTT CCTAAAAGCG AGACTCGTTT GCTCAGTACC AGGAATGAAT 
GGAATTGACA CATATTTTGA TGAATTAGAG GACGTTTTTT TGCTACCTAC CAGAGATCAT 
AAGAATCCAG TGATATTTGG ACTCTTTAAC ACTACCAGTA ATATTTTTCG AGGGCATGCT 
ATATGTGTCT ATCACATGTC TAGCATTCGG GCAGCCTTCA ACGGACCATA TGCACATAAG 
GAAGGACCTG AATACCACTG GTCAGTCTAT GAAGGAAAAG TCCCTTATCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTAAA TGGAGGGAGA TACGGAACCA CCAAGGACTA TCCTGATGAT 
GCCATCCGAT TTGCAAGAAG TCATCCACTA ATGTACCAGG CCATAAAACC TGCCCATAAA 
AAACCAATAT TGGTAAAAAC AGATGGAAAA TATAACCTGA AACAAATAGC AGTAGATCGA 
GTGGAAGCTG AGGATGGCCA ATATGACGTC TTGTTTATTG GGACAGATAA TGGAATTGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ATGGAATCAA TGGAAGAAGT AATTCTAGAA 
GAACTTCAGA TATTCAAGGA TCCAGTTCCT ATTATTTCTA TGGAGATTTC TTCAAAGCGG 
CAACAGCTGT ATATTGGATC TGCTTCTGCT GTGGCTCAAG TCAGATTCCA TCACTGTGAC 
ATGTATGGAA GTGCTTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGCATATCCT GCTCCCGGTA TTACCCAACA GGCACACATG CAAAAAGGCG TTTCCGGAGA 
CAAGATGTTC GACATGGAAA TGCAGCTCAG CAGTGCTTTG GACAACAGTT TGTTGGGGAT 
GCTTTGGATA AGACTGAAGA ACATCTGGCT TATGGCATAG AGAACAACAG TACTTTGCTG 
GAATGTACCC CACGATCTTT ACAAGCGAAA GTTATCTGGT TTGTACAGAA AGGACGTGAG 
ACAAGAAAAG AGGAGGTGAA GACAGATGAC AGAGTGGTTA AGATGGACCT TGGTTTACTC 
TTCCTAAGGT TACACAAATC AGATGCTGGG ACCTATTTTT GCCAGACAGT AGAGCATAGC 
TTTGTCCATA CGGTCCGTAA AATCACCTTG GAGGTAGTGG AAGAGGAGAA AGTCGAGGAT 
ATGTTTAACA AGGACGATGA GGAGGACAGG CATCACAGGA TGCCTTGTCC TGCTCAGAGT 
AGCATCTCGC AGGGAGCAAA ACCATGGTAC AAGGAATTCT TGCAGCTGAT CGGTTATAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAG AAAGTATGGT GCACAGATAG AAAGAGGAAA 
AAGCTTAAAA TGTCACCCTC CAAGTGGAAG TATGCCAACC CTCAGGAAAA GAAGCTCCGT 
TCCAAACCTG AGCATTACCG CCTGCCCAGG CACACGCTGG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3E ( NM_012431.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_036563.1 Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22789
Clone ID Related Accession (Same CDS sequence)	NM_012431.2 , NM_012431.3
Accession Version	NM_012431.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	semaphorin-3E isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB215551.1, BC152458.1, DA722912.1, BC140706.1 and AC079799.7. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_012431.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002329.1, SRR1660807.77724.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCCG CGGGGCACAT TATCACCTTG CTCCTGTGGG GTTACTTACT GGAGCTTTGG 
ACAGGAGGTC ATACAGCTGA TACTACCCAC CCCCGGTTAC GCCTGTCACA TAAAGAGCTC 
TTGAATCTGA ACAGAACATC AATATTTCAT AGCCCTTTTG GATTTCTTGA TCTCCATACA 
ATGCTGCTGG ATGAATATCA AGAGAGGCTC TTCGTGGGAG GCAGGGACCT TGTATATTCC 
CTCAGCTTGG AGAGAATCAG TGACGGCTAT AAAGAGATAC ACTGGCCGAG TACAGCTCTA 
AAAATGGAAG AATGCATAAT GAAGGGAAAA GATGCGGGTG AATGTGCAAA TTATGTTCGG 
GTTTTGCATC ACTATAACAG GACACACCTT CTGACCTGTG GTACTGGAGC TTTTGATCCA 
GTTTGTGCCT TCATCAGAGT TGGATATCAT TTGGAGGATC CTCTGTTTCA CCTGGAATCA 
CCCAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAGCTCCTC CTTCATCTCC 
ACTTTAATTG GTAGTGAATT GTTTGCTGGA CTCTACAGTG ACTACTGGAG CAGAGACGCT 
GCGATCTTCC GCAGCATGGG GCGACTGGCC CATATCCGCA CTGAGCATGA CGATGAGCGT 
CTGTTGAAAG AACCAAAATT TGTAGGTTCA TACATGATTC CTGACAATGA AGACAGAGAT 
GACAACAAAG TATATTTCTT TTTTACTGAG AAGGCACTGG AGGCAGAAAA CAATGCTCAC 
GCAATTTACA CCAGGGTCGG GCGACTCTGT GTGAATGATG TAGGAGGGCA GAGAATACTG 
GTGAATAAGT GGAGCACTTT CCTAAAAGCG AGACTCGTTT GCTCAGTACC AGGAATGAAT 
GGAATTGACA CATATTTTGA TGAATTAGAG GACGTTTTTT TGCTACCTAC CAGAGATCAT 
AAGAATCCAG TGATATTTGG ACTCTTTAAC ACTACCAGTA ATATTTTTCG AGGGCATGCT 
ATATGTGTCT ATCACATGTC TAGCATTCGG GCAGCCTTCA ACGGACCATA TGCACATAAG 
GAAGGACCTG AATACCACTG GTCAGTCTAT GAAGGAAAAG TCCCTTATCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTAAA TGGAGGGAGA TACGGAACCA CCAAGGACTA TCCTGATGAT 
GCCATCCGAT TTGCAAGAAG TCATCCACTA ATGTACCAGG CCATAAAACC TGCCCATAAA 
AAACCAATAT TGGTAAAAAC AGATGGAAAA TATAACCTGA AACAAATAGC AGTAGATCGA 
GTGGAAGCTG AGGATGGCCA ATATGACGTC TTGTTTATTG GGACAGATAA TGGAATTGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ATGGAATCAA TGGAAGAAGT AATTCTAGAA 
GAACTTCAGA TATTCAAGGA TCCAGTTCCT ATTATTTCTA TGGAGATTTC TTCAAAGCGG 
CAACAGCTGT ATATTGGATC TGCTTCTGCT GTGGCTCAAG TCAGATTCCA TCACTGTGAC 
ATGTATGGAA GTGCTTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGCATATCCT GCTCCCGGTA TTACCCAACA GGCACACATG CAAAAAGGCG TTTCCGGAGA 
CAAGATGTTC GACATGGAAA TGCAGCTCAG CAGTGCTTTG GACAACAGTT TGTTGGGGAT 
GCTTTGGATA AGACTGAAGA ACATCTGGCT TATGGCATAG AGAACAACAG TACTTTGCTG 
GAATGTACCC CACGATCTTT ACAAGCGAAA GTTATCTGGT TTGTACAGAA AGGACGTGAG 
ACAAGAAAAG AGGAGGTGAA GACAGATGAC AGAGTGGTTA AGATGGACCT TGGTTTACTC 
TTCCTAAGGT TACACAAATC AGATGCTGGG ACCTATTTTT GCCAGACAGT AGAGCATAGC 
TTTGTCCATA CGGTCCGTAA AATCACCTTG GAGGTAGTGG AAGAGGAGAA AGTCGAGGAT 
ATGTTTAACA AGGACGATGA GGAGGACAGG CATCACAGGA TGCCTTGTCC TGCTCAGAGT 
AGCATCTCGC AGGGAGCAAA ACCATGGTAC AAGGAATTCT TGCAGCTGAT CGGTTATAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAG AAAGTATGGT GCACAGATAG AAAGAGGAAA 
AAGCTTAAAA TGTCACCCTC CAAGTGGAAG TATGCCAACC CTCAGGAAAA GAAGCTCCGT 
TCCAAACCTG AGCATTACCG CCTGCCCAGG CACACGCTGG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036563.1
CDS	622..2949
Translation	MASAGHIITLLLWGYLLELWTGGHTADTTHPRLRLSHKELLNLNRTSIFHSPFGFLDLHTMLLDEYQERLFVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHLLTCGTGAFDPVCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDAAIFRSMGRLAHIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLCVNDVGGQRILVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHAICVYHMSSIRAAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPLMYQAIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILEELQIFKDPVPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGTHAKRRFRRQDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRETRKEEVKTDDRVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDRHHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLRSKPEHYRLPRHTLDS

Target ORF information:

RefSeq Version	NM_012431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012431.2

ORF Insert Sequence:

ATGGCATCCG CGGGGCACAT TATCACCTTG CTCCTGTGGG GTTACTTACT GGAGCTTTGG 
ACAGGAGGTC ATACAGCTGA TACTACCCAC CCCCGGTTAC GCCTGTCACA TAAAGAGCTC 
TTGAATCTGA ACAGAACATC AATATTTCAT AGCCCTTTTG GATTTCTTGA TCTCCATACA 
ATGCTGCTGG ATGAATATCA AGAGAGGCTC TTCGTGGGAG GCAGGGACCT TGTATATTCC 
CTCAGCTTGG AGAGAATCAG TGACGGCTAT AAAGAGATAC ACTGGCCGAG TACAGCTCTA 
AAAATGGAAG AATGCATAAT GAAGGGAAAA GATGCGGGTG AATGTGCAAA TTATGTTCGG 
GTTTTGCATC ACTATAACAG GACACACCTT CTGACCTGTG GTACTGGAGC TTTTGATCCA 
GTTTGTGCCT TCATCAGAGT TGGATATCAT TTGGAGGATC CTCTGTTTCA CCTGGAATCA 
CCCAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAGCTCCTC CTTCATCTCC 
ACTTTAATTG GTAGTGAATT GTTTGCTGGA CTCTACAGTG ACTACTGGAG CAGAGACGCT 
GCGATCTTCC GCAGCATGGG GCGACTGGCC CATATCCGCA CTGAGCATGA CGATGAGCGT 
CTGTTGAAAG AACCAAAATT TGTAGGTTCA TACATGATTC CTGACAATGA AGACAGAGAT 
GACAACAAAG TATATTTCTT TTTTACTGAG AAGGCACTGG AGGCAGAAAA CAATGCTCAC 
GCAATTTACA CCAGGGTCGG GCGACTCTGT GTGAATGATG TAGGAGGGCA GAGAATACTG 
GTGAATAAGT GGAGCACTTT CCTAAAAGCG AGACTCGTTT GCTCAGTACC AGGAATGAAT 
GGAATTGACA CATATTTTGA TGAATTAGAG GACGTTTTTT TGCTACCTAC CAGAGATCAT 
AAGAATCCAG TGATATTTGG ACTCTTTAAC ACTACCAGTA ATATTTTTCG AGGGCATGCT 
ATATGTGTCT ATCACATGTC TAGCATTCGG GCAGCCTTCA ACGGACCATA TGCACATAAG 
GAAGGACCTG AATACCACTG GTCAGTCTAT GAAGGAAAAG TCCCTTATCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTAAA TGGAGGGAGA TACGGAACCA CCAAGGACTA TCCTGATGAT 
GCCATCCGAT TTGCAAGAAG TCATCCACTA ATGTACCAGG CCATAAAACC TGCCCATAAA 
AAACCAATAT TGGTAAAAAC AGATGGAAAA TATAACCTGA AACAAATAGC AGTAGATCGA 
GTGGAAGCTG AGGATGGCCA ATATGACGTC TTGTTTATTG GGACAGATAA TGGAATTGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ATGGAATCAA TGGAAGAAGT AATTCTAGAA 
GAACTTCAGA TATTCAAGGA TCCAGTTCCT ATTATTTCTA TGGAGATTTC TTCAAAGCGG 
CAACAGCTGT ATATTGGATC TGCTTCTGCT GTGGCTCAAG TCAGATTCCA TCACTGTGAC 
ATGTATGGAA GTGCTTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGCATATCCT GCTCCCGGTA TTACCCAACA GGCACACATG CAAAAAGGCG TTTCCGGAGA 
CAAGATGTTC GACATGGAAA TGCAGCTCAG CAGTGCTTTG GACAACAGTT TGTTGGGGAT 
GCTTTGGATA AGACTGAAGA ACATCTGGCT TATGGCATAG AGAACAACAG TACTTTGCTG 
GAATGTACCC CACGATCTTT ACAAGCGAAA GTTATCTGGT TTGTACAGAA AGGACGTGAG 
ACAAGAAAAG AGGAGGTGAA GACAGATGAC AGAGTGGTTA AGATGGACCT TGGTTTACTC 
TTCCTAAGGT TACACAAATC AGATGCTGGG ACCTATTTTT GCCAGACAGT AGAGCATAGC 
TTTGTCCATA CGGTCCGTAA AATCACCTTG GAGGTAGTGG AAGAGGAGAA AGTCGAGGAT 
ATGTTTAACA AGGACGATGA GGAGGACAGG CATCACAGGA TGCCTTGTCC TGCTCAGAGT 
AGCATCTCGC AGGGAGCAAA ACCATGGTAC AAGGAATTCT TGCAGCTGAT CGGTTATAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAG AAAGTATGGT GCACAGATAG AAAGAGGAAA 
AAGCTTAAAA TGTCACCCTC CAAGTGGAAG TATGCCAACC CTCAGGAAAA GAAGCTCCGT 
TCCAAACCTG AGCATTACCG CCTGCCCAGG CACACGCTGG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA3E ( NM_012431.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036563.1 Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.

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CloneID	OHu22789
Clone ID Related Accession (Same CDS sequence)	NM_012431.2 , NM_012431.3
Accession Version	NM_012431.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	semaphorin-3E isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB215551.1, BC152458.1, DA722912.1, BC140706.1, AC079799.7 and AW450461.1. This sequence is a reference standard in the RefSeqGene project. On Oct 1, 2018 this sequence version replaced NM_012431.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB002329.1, BC152458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2467144 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000643230.2/ ENSP00000496491.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGCATCCG CGGGGCACAT TATCACCTTG CTCCTGTGGG GTTACTTACT GGAGCTTTGG 
ACAGGAGGTC ATACAGCTGA TACTACCCAC CCCCGGTTAC GCCTGTCACA TAAAGAGCTC 
TTGAATCTGA ACAGAACATC AATATTTCAT AGCCCTTTTG GATTTCTTGA TCTCCATACA 
ATGCTGCTGG ATGAATATCA AGAGAGGCTC TTCGTGGGAG GCAGGGACCT TGTATATTCC 
CTCAGCTTGG AGAGAATCAG TGACGGCTAT AAAGAGATAC ACTGGCCGAG TACAGCTCTA 
AAAATGGAAG AATGCATAAT GAAGGGAAAA GATGCGGGTG AATGTGCAAA TTATGTTCGG 
GTTTTGCATC ACTATAACAG GACACACCTT CTGACCTGTG GTACTGGAGC TTTTGATCCA 
GTTTGTGCCT TCATCAGAGT TGGATATCAT TTGGAGGATC CTCTGTTTCA CCTGGAATCA 
CCCAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAGCTCCTC CTTCATCTCC 
ACTTTAATTG GTAGTGAATT GTTTGCTGGA CTCTACAGTG ACTACTGGAG CAGAGACGCT 
GCGATCTTCC GCAGCATGGG GCGACTGGCC CATATCCGCA CTGAGCATGA CGATGAGCGT 
CTGTTGAAAG AACCAAAATT TGTAGGTTCA TACATGATTC CTGACAATGA AGACAGAGAT 
GACAACAAAG TATATTTCTT TTTTACTGAG AAGGCACTGG AGGCAGAAAA CAATGCTCAC 
GCAATTTACA CCAGGGTCGG GCGACTCTGT GTGAATGATG TAGGAGGGCA GAGAATACTG 
GTGAATAAGT GGAGCACTTT CCTAAAAGCG AGACTCGTTT GCTCAGTACC AGGAATGAAT 
GGAATTGACA CATATTTTGA TGAATTAGAG GACGTTTTTT TGCTACCTAC CAGAGATCAT 
AAGAATCCAG TGATATTTGG ACTCTTTAAC ACTACCAGTA ATATTTTTCG AGGGCATGCT 
ATATGTGTCT ATCACATGTC TAGCATTCGG GCAGCCTTCA ACGGACCATA TGCACATAAG 
GAAGGACCTG AATACCACTG GTCAGTCTAT GAAGGAAAAG TCCCTTATCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTAAA TGGAGGGAGA TACGGAACCA CCAAGGACTA TCCTGATGAT 
GCCATCCGAT TTGCAAGAAG TCATCCACTA ATGTACCAGG CCATAAAACC TGCCCATAAA 
AAACCAATAT TGGTAAAAAC AGATGGAAAA TATAACCTGA AACAAATAGC AGTAGATCGA 
GTGGAAGCTG AGGATGGCCA ATATGACGTC TTGTTTATTG GGACAGATAA TGGAATTGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ATGGAATCAA TGGAAGAAGT AATTCTAGAA 
GAACTTCAGA TATTCAAGGA TCCAGTTCCT ATTATTTCTA TGGAGATTTC TTCAAAGCGG 
CAACAGCTGT ATATTGGATC TGCTTCTGCT GTGGCTCAAG TCAGATTCCA TCACTGTGAC 
ATGTATGGAA GTGCTTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGCATATCCT GCTCCCGGTA TTACCCAACA GGCACACATG CAAAAAGGCG TTTCCGGAGA 
CAAGATGTTC GACATGGAAA TGCAGCTCAG CAGTGCTTTG GACAACAGTT TGTTGGGGAT 
GCTTTGGATA AGACTGAAGA ACATCTGGCT TATGGCATAG AGAACAACAG TACTTTGCTG 
GAATGTACCC CACGATCTTT ACAAGCGAAA GTTATCTGGT TTGTACAGAA AGGACGTGAG 
ACAAGAAAAG AGGAGGTGAA GACAGATGAC AGAGTGGTTA AGATGGACCT TGGTTTACTC 
TTCCTAAGGT TACACAAATC AGATGCTGGG ACCTATTTTT GCCAGACAGT AGAGCATAGC 
TTTGTCCATA CGGTCCGTAA AATCACCTTG GAGGTAGTGG AAGAGGAGAA AGTCGAGGAT 
ATGTTTAACA AGGACGATGA GGAGGACAGG CATCACAGGA TGCCTTGTCC TGCTCAGAGT 
AGCATCTCGC AGGGAGCAAA ACCATGGTAC AAGGAATTCT TGCAGCTGAT CGGTTATAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAG AAAGTATGGT GCACAGATAG AAAGAGGAAA 
AAGCTTAAAA TGTCACCCTC CAAGTGGAAG TATGCCAACC CTCAGGAAAA GAAGCTCCGT 
TCCAAACCTG AGCATTACCG CCTGCCCAGG CACACGCTGG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036563.1
CDS	598..2925
Translation	MASAGHIITLLLWGYLLELWTGGHTADTTHPRLRLSHKELLNLNRTSIFHSPFGFLDLHTMLLDEYQERLFVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHLLTCGTGAFDPVCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDAAIFRSMGRLAHIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLCVNDVGGQRILVNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHAICVYHMSSIRAAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPLMYQAIKPAHKKPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILEELQIFKDPVPIISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGTHAKRRFRRQDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRETRKEEVKTDDRVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDRHHRMPCPAQSSISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLRSKPEHYRLPRHTLDS

Target ORF information:

RefSeq Version	NM_012431.3
Organism	Homo sapiens(human)
Definition	Homo sapiens semaphorin 3E (SEMA3E), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012431.3

ORF Insert Sequence:

ATGGCATCCG CGGGGCACAT TATCACCTTG CTCCTGTGGG GTTACTTACT GGAGCTTTGG 
ACAGGAGGTC ATACAGCTGA TACTACCCAC CCCCGGTTAC GCCTGTCACA TAAAGAGCTC 
TTGAATCTGA ACAGAACATC AATATTTCAT AGCCCTTTTG GATTTCTTGA TCTCCATACA 
ATGCTGCTGG ATGAATATCA AGAGAGGCTC TTCGTGGGAG GCAGGGACCT TGTATATTCC 
CTCAGCTTGG AGAGAATCAG TGACGGCTAT AAAGAGATAC ACTGGCCGAG TACAGCTCTA 
AAAATGGAAG AATGCATAAT GAAGGGAAAA GATGCGGGTG AATGTGCAAA TTATGTTCGG 
GTTTTGCATC ACTATAACAG GACACACCTT CTGACCTGTG GTACTGGAGC TTTTGATCCA 
GTTTGTGCCT TCATCAGAGT TGGATATCAT TTGGAGGATC CTCTGTTTCA CCTGGAATCA 
CCCAGATCTG AGAGAGGAAG GGGCAGATGT CCTTTTGACC CCAGCTCCTC CTTCATCTCC 
ACTTTAATTG GTAGTGAATT GTTTGCTGGA CTCTACAGTG ACTACTGGAG CAGAGACGCT 
GCGATCTTCC GCAGCATGGG GCGACTGGCC CATATCCGCA CTGAGCATGA CGATGAGCGT 
CTGTTGAAAG AACCAAAATT TGTAGGTTCA TACATGATTC CTGACAATGA AGACAGAGAT 
GACAACAAAG TATATTTCTT TTTTACTGAG AAGGCACTGG AGGCAGAAAA CAATGCTCAC 
GCAATTTACA CCAGGGTCGG GCGACTCTGT GTGAATGATG TAGGAGGGCA GAGAATACTG 
GTGAATAAGT GGAGCACTTT CCTAAAAGCG AGACTCGTTT GCTCAGTACC AGGAATGAAT 
GGAATTGACA CATATTTTGA TGAATTAGAG GACGTTTTTT TGCTACCTAC CAGAGATCAT 
AAGAATCCAG TGATATTTGG ACTCTTTAAC ACTACCAGTA ATATTTTTCG AGGGCATGCT 
ATATGTGTCT ATCACATGTC TAGCATTCGG GCAGCCTTCA ACGGACCATA TGCACATAAG 
GAAGGACCTG AATACCACTG GTCAGTCTAT GAAGGAAAAG TCCCTTATCC AAGGCCTGGT 
TCTTGTGCCA GCAAAGTAAA TGGAGGGAGA TACGGAACCA CCAAGGACTA TCCTGATGAT 
GCCATCCGAT TTGCAAGAAG TCATCCACTA ATGTACCAGG CCATAAAACC TGCCCATAAA 
AAACCAATAT TGGTAAAAAC AGATGGAAAA TATAACCTGA AACAAATAGC AGTAGATCGA 
GTGGAAGCTG AGGATGGCCA ATATGACGTC TTGTTTATTG GGACAGATAA TGGAATTGTG 
CTGAAAGTAA TCACAATTTA CAACCAAGAA ATGGAATCAA TGGAAGAAGT AATTCTAGAA 
GAACTTCAGA TATTCAAGGA TCCAGTTCCT ATTATTTCTA TGGAGATTTC TTCAAAGCGG 
CAACAGCTGT ATATTGGATC TGCTTCTGCT GTGGCTCAAG TCAGATTCCA TCACTGTGAC 
ATGTATGGAA GTGCTTGTGC TGACTGCTGC CTGGCTCGAG ACCCTTACTG TGCCTGGGAT 
GGCATATCCT GCTCCCGGTA TTACCCAACA GGCACACATG CAAAAAGGCG TTTCCGGAGA 
CAAGATGTTC GACATGGAAA TGCAGCTCAG CAGTGCTTTG GACAACAGTT TGTTGGGGAT 
GCTTTGGATA AGACTGAAGA ACATCTGGCT TATGGCATAG AGAACAACAG TACTTTGCTG 
GAATGTACCC CACGATCTTT ACAAGCGAAA GTTATCTGGT TTGTACAGAA AGGACGTGAG 
ACAAGAAAAG AGGAGGTGAA GACAGATGAC AGAGTGGTTA AGATGGACCT TGGTTTACTC 
TTCCTAAGGT TACACAAATC AGATGCTGGG ACCTATTTTT GCCAGACAGT AGAGCATAGC 
TTTGTCCATA CGGTCCGTAA AATCACCTTG GAGGTAGTGG AAGAGGAGAA AGTCGAGGAT 
ATGTTTAACA AGGACGATGA GGAGGACAGG CATCACAGGA TGCCTTGTCC TGCTCAGAGT 
AGCATCTCGC AGGGAGCAAA ACCATGGTAC AAGGAATTCT TGCAGCTGAT CGGTTATAGC 
AACTTCCAGA GAGTGGAAGA ATACTGCGAG AAAGTATGGT GCACAGATAG AAAGAGGAAA 
AAGCTTAAAA TGTCACCCTC CAAGTGGAAG TATGCCAACC CTCAGGAAAA GAAGCTCCGT 
TCCAAACCTG AGCATTACCG CCTGCCCAGG CACACGCTGG ACTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of semaphorin 3E is suppressed in severe asthma.
The Journal of allergy and clinical immunology140(4)1176-1179(2017 Oct)
Movassagh H,Shan L,Chakir J,McConville JF,Halayko AJ,Koussih L,Gounni AS

Chemorepellent Semaphorin 3E Negatively Regulates Neutrophil Migration In Vitro and In Vivo.
Journal of immunology (Baltimore, Md. : 1950)198(3)1023-1033(2017 Feb)
Movassagh H,Saati A,Nandagopal S,Mohammed A,Tatari N,Shan L,Duke-Cohan JS,Fowke KR,Lin F,Gounni AS

Association of Increased Serum Sema3E with TRIB3 Q84R Polymorphism and Carotid Atherosclerosis in Metabolic Syndrome.
Annals of clinical and laboratory science47(1)47-51(2017 Jan)
Qin RR,Song M,Li YH,Wang F,Zhou HM,Liu MH,Zhong M,Zhang Y,Zhang W,Wang ZH

Overexpression of Semaphorin-3E enhances pancreatic cancer cell growth and associates with poor patient survival.
Oncotarget7(52)87431-87448(2016 Dec)
Yong LK,Lai S,Liang Z,Poteet E,Chen F,van Buren G,Fisher W,Mo Q,Chen C,Yao Q

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SEMA3E cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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