TRAM2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRAM2
Entrez Gene ID	9697
Full Name	translocation associated membrane protein 2
General protein information	Preferred Names translocation associated membrane protein 2 Names translocating chain-associated membrane protein 2 TRAM-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p12.2
Summary	TRAM2 is a component of the translocon, a gated macromolecular channel that controls the posttranslational processing of nascent secretory and membrane proteins at the endoplasmic reticulum (ER) membrane.[supplied by OMIM, Jul 2004].
Disorder MIM:	608485

mRNA and Protein(s)

mRNA	Protein	Name
XM_011515005.2	XP_011513307.1	translocating chain-associated membrane protein 2 isoform X1
NM_012288.3	NP_036420.1	translocating chain-associated membrane protein 2
NM_012288.4	NP_036420.1	translocating chain-associated membrane protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRAM2	XP_518539.2
Xenopus tropicalis (tropical clawed frog)	tram2	NP_001135493.1
	TRAM2	XP_001107706.1
Bos taurus (cattle)	TRAM2	NP_001180079.1
Canis lupus familiaris (dog)	TRAM2	XP_005627826.1
Mus musculus (house mouse)	Tram2	NP_803128.1
Danio rerio (zebrafish)	tram2	NP_998236.1
Homo sapiens (human)	TRAM2	NP_036420.1
Rattus norvegicus (Norway rat)	LOC100361830	XP_002730055.2
Gallus gallus (chicken)	TRAM2	XP_420046.4

Gene Ontology
Bibliography

Related articles in PubMed

Inverting the Topology of a Transmembrane Protein by Regulating the Translocation of the First Transmembrane Helix.
Chen Q, Denard B, Lee CE, Han S, Ye JS, Ye J
Molecular cell63(4)567-578(2016 Aug)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

DAP12 Stabilizes the C-terminal Fragment of the Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) and Protects against LPS-induced Pro-inflammatory Response.
Zhong L, Chen XF, Zhang ZL, Wang Z, Shi XZ, Xu K, Zhang YW, Xu H, Bu G
The Journal of biological chemistry290(25)15866-77(2015 Jun)

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Oshikawa M, Tsutsui C, Ikegami T, Fuchida Y, Matsubara M, Toyama S, Usami R, Ohtoko K, Kato S
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)

Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
Trynka G, Zhernakova A, Romanos J, Franke L, Hunt KA, Turner G, Bruinenberg M, Heap GA, Platteel M, Ryan AW, de Kovel C, Holmes GK, Howdle PD, Walters JR, Sanders DS, Mulder CJ, Mearin ML, Verbeek WH, Trimble V, Stevens FM, Kelleher D, Barisani D, Bardella MT, McManus R, van Heel DA, Wijmenga C
Gut58(8)1078-83(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results support a role of DAP12 in stabilizing TREM2-CTF, thereby protecting against excessive pro-inflammatory responses.
Title: DAP12 Stabilizes the C-terminal Fragment of the Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) and Protects against LPS-induced Pro-inflammatory Response.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.

TRAM2, as a part of the translocon, is required for the biosynthesis of type I collagen by coupling the activity of SERCA2b with the activity of the translocon
Title: TRAM2 protein interacts with endoplasmic reticulum Ca2+ pump Serca2b and is necessary for collagen type I synthesis.

The following TRAM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRAM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06158	NM_012288.4 Latest version!	Homo sapiens translocation associated membrane protein 2 (TRAM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu63170	XM_011515005.2 Latest version!	Homo sapiens translocation associated membrane protein 2 (TRAM2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu06158	NM_012288.3	Homo sapiens translocation associated membrane protein 2 (TRAM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRAM2 ( NM_012288.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036420.1 Homo sapiens translocation associated membrane protein 2 (TRAM2), mRNA.

Price & Availability↑

CloneID	OHu06158
Clone ID Related Accession (Same CDS sequence)	NM_012288.3 , NM_012288.4
Accession Version	NM_012288.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	translocating chain-associated membrane protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028121.1, D31762.1, AL049611.24 and AA044636.1. On Dec 10, 2004 this sequence version replaced NM_012288.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028121.1, D31762.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTCC GCAGGAGGAC GAAAAGTTAC CCGCTCTTCA GCCAGGAGTT CGTCATCCAC 
AACCATGCGG ACATCGGCTT CTGCCTGGTG CTCTGCGTCC TCATCGGGCT TATGTTCGAG 
GTCACAGCCA AGACTGCCTT TCTATTTATT TTACCTCAGT ATAACATTAG CGTGCCTACA 
GCAGACAGTG AGACCGTGCA CTACCACTAT GGCCCTAAGG ACCTGGTCAC AATCTTGTTC 
TACATCTTCA TCACCATCAT CTTGCATGCT GTGGTTCAGG AGTACATTTT AGATAAAATC 
AGCAAACGGC TTCATCTCTC CAAAGTCAAA CACAGCAAGT TCAATGAATC TGGACAGCTG 
GTCGTCTTTC ATTTCACCTC GGTGATTTGG TGCTTCTACG TGGTGGTGAC GGAAGGATAC 
TTAACAAACC CAAGAAGCCT CTGGGAAGAC TACCCGCATG TGCACCTCCC CTTCCAGGTG 
AAGTTTTTCT ACCTATGCCA GCTGGCCTAC TGGCTGCACG CACTTCCTGA GCTATACTTC 
CAGAAGGTAC GGAAGGAGGA AATTCCCCGC CAGCTCCAGT ATATTTGCCT GTACCTGGTG 
CATATAGCTG GAGCATACCT CTTAAACCTG AGCCGCCTGG GCCTGATCTT GCTGCTGCTG 
CAGTACTCAA CTGAGTTCCT CTTCCACACG GCTAGACTCT TCTACTTTGC AGATGAAAAC 
AACGAGAAAC TGTTCAGTGC CTGGGCTGCT GTTTTTGGGG TTACCCGCCT CTTCATCCTC 
ACCCTTGCCG TGCTGGCCAT TGGCTTTGGA CTGGCTCGCA TGGAAAACCA GGCATTTGAT 
CCCGAGAAAG GGAACTTCAA CACTTTGTTT TGCAGGCTCT GCGTGCTGCT GCTGGTGTGT 
GCCGCCCAGG CCTGGCTCAT GTGGCGCTTC ATCCACTCCC AGCTGCGGCA CTGGCGGGAA 
TACTGGAATG AGCAGAGTGC AAAGCGGAGA GTCCCAGCCA CACCCAGACT ACCAGCCAGG 
CTCATCAAGA GGGAATCTGG TTACCATGAA AATGGAGTGG TGAAGGCAGA GAACGGAACC 
TCCCCACGGA CTAAGAAACT CAAGTCTCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036420.1
CDS	150..1262
Translation	MAFRRRTKSYPLFSQEFVIHNHADIGFCLVLCVLIGLMFEVTAKTAFLFILPQYNISVPTADSETVHYHYGPKDLVTILFYIFITIILHAVVQEYILDKISKRLHLSKVKHSKFNESGQLVVFHFTSVIWCFYVVVTEGYLTNPRSLWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLVHIAGAYLLNLSRLGLILLLLQYSTEFLFHTARLFYFADENNEKLFSAWAAVFGVTRLFILTLAVLAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVCAAQAWLMWRFIHSQLRHWREYWNEQSAKRRVPATPRLPARLIKRESGYHENGVVKAENGTSPRTKKLKSP

Target ORF information:

RefSeq Version	NM_012288.3
Organism	Homo sapiens(human)
Definition	Homo sapiens translocation associated membrane protein 2 (TRAM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012288.3

ORF Insert Sequence:

ATGGCTTTCC GCAGGAGGAC GAAAAGTTAC CCGCTCTTCA GCCAGGAGTT CGTCATCCAC 
AACCATGCGG ACATCGGCTT CTGCCTGGTG CTCTGCGTCC TCATCGGGCT TATGTTCGAG 
GTCACAGCCA AGACTGCCTT TCTATTTATT TTACCTCAGT ATAACATTAG CGTGCCTACA 
GCAGACAGTG AGACCGTGCA CTACCACTAT GGCCCTAAGG ACCTGGTCAC AATCTTGTTC 
TACATCTTCA TCACCATCAT CTTGCATGCT GTGGTTCAGG AGTACATTTT AGATAAAATC 
AGCAAACGGC TTCATCTCTC CAAAGTCAAA CACAGCAAGT TCAATGAATC TGGACAGCTG 
GTCGTCTTTC ATTTCACCTC GGTGATTTGG TGCTTCTACG TGGTGGTGAC GGAAGGATAC 
TTAACAAACC CAAGAAGCCT CTGGGAAGAC TACCCGCATG TGCACCTCCC CTTCCAGGTG 
AAGTTTTTCT ACCTATGCCA GCTGGCCTAC TGGCTGCACG CACTTCCTGA GCTATACTTC 
CAGAAGGTAC GGAAGGAGGA AATTCCCCGC CAGCTCCAGT ATATTTGCCT GTACCTGGTG 
CATATAGCTG GAGCATACCT CTTAAACCTG AGCCGCCTGG GCCTGATCTT GCTGCTGCTG 
CAGTACTCAA CTGAGTTCCT CTTCCACACG GCTAGACTCT TCTACTTTGC AGATGAAAAC 
AACGAGAAAC TGTTCAGTGC CTGGGCTGCT GTTTTTGGGG TTACCCGCCT CTTCATCCTC 
ACCCTTGCCG TGCTGGCCAT TGGCTTTGGA CTGGCTCGCA TGGAAAACCA GGCATTTGAT 
CCCGAGAAAG GGAACTTCAA CACTTTGTTT TGCAGGCTCT GCGTGCTGCT GCTGGTGTGT 
GCCGCCCAGG CCTGGCTCAT GTGGCGCTTC ATCCACTCCC AGCTGCGGCA CTGGCGGGAA 
TACTGGAATG AGCAGAGTGC AAAGCGGAGA GTCCCAGCCA CACCCAGACT ACCAGCCAGG 
CTCATCAAGA GGGAATCTGG TTACCATGAA AATGGAGTGG TGAAGGCAGA GAACGGAACC 
TCCCCACGGA CTAAGAAACT CAAGTCTCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRAM2 ( NM_012288.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036420.1 Homo sapiens translocation associated membrane protein 2 (TRAM2), mRNA.

Price & Availability↑

CloneID	OHu06158
Clone ID Related Accession (Same CDS sequence)	NM_012288.3 , NM_012288.4
Accession Version	NM_012288.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1113bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	translocating chain-associated membrane protein 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028121.1, D31762.1 and AL049611.24. On Nov 22, 2018 this sequence version replaced NM_012288.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D31762.1, SRR1803614.72826.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000182527.4/ ENSP00000182527.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTTCC GCAGGAGGAC GAAAAGTTAC CCGCTCTTCA GCCAGGAGTT CGTCATCCAC 
AACCATGCGG ACATCGGCTT CTGCCTGGTG CTCTGCGTCC TCATCGGGCT TATGTTCGAG 
GTCACAGCCA AGACTGCCTT TCTATTTATT TTACCTCAGT ATAACATTAG CGTGCCTACA 
GCAGACAGTG AGACCGTGCA CTACCACTAT GGCCCTAAGG ACCTGGTCAC AATCTTGTTC 
TACATCTTCA TCACCATCAT CTTGCATGCT GTGGTTCAGG AGTACATTTT AGATAAAATC 
AGCAAACGGC TTCATCTCTC CAAAGTCAAA CACAGCAAGT TCAATGAATC TGGACAGCTG 
GTCGTCTTTC ATTTCACCTC GGTGATTTGG TGCTTCTACG TGGTGGTGAC GGAAGGATAC 
TTAACAAACC CAAGAAGCCT CTGGGAAGAC TACCCGCATG TGCACCTCCC CTTCCAGGTG 
AAGTTTTTCT ACCTATGCCA GCTGGCCTAC TGGCTGCACG CACTTCCTGA GCTATACTTC 
CAGAAGGTAC GGAAGGAGGA AATTCCCCGC CAGCTCCAGT ATATTTGCCT GTACCTGGTG 
CATATAGCTG GAGCATACCT CTTAAACCTG AGCCGCCTGG GCCTGATCTT GCTGCTGCTG 
CAGTACTCAA CTGAGTTCCT CTTCCACACG GCTAGACTCT TCTACTTTGC AGATGAAAAC 
AACGAGAAAC TGTTCAGTGC CTGGGCTGCT GTTTTTGGGG TTACCCGCCT CTTCATCCTC 
ACCCTTGCCG TGCTGGCCAT TGGCTTTGGA CTGGCTCGCA TGGAAAACCA GGCATTTGAT 
CCCGAGAAAG GGAACTTCAA CACTTTGTTT TGCAGGCTCT GCGTGCTGCT GCTGGTGTGT 
GCCGCCCAGG CCTGGCTCAT GTGGCGCTTC ATCCACTCCC AGCTGCGGCA CTGGCGGGAA 
TACTGGAATG AGCAGAGTGC AAAGCGGAGA GTCCCAGCCA CACCCAGACT ACCAGCCAGG 
CTCATCAAGA GGGAATCTGG TTACCATGAA AATGGAGTGG TGAAGGCAGA GAACGGAACC 
TCCCCACGGA CTAAGAAACT CAAGTCTCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036420.1
CDS	146..1258
Translation	MAFRRRTKSYPLFSQEFVIHNHADIGFCLVLCVLIGLMFEVTAKTAFLFILPQYNISVPTADSETVHYHYGPKDLVTILFYIFITIILHAVVQEYILDKISKRLHLSKVKHSKFNESGQLVVFHFTSVIWCFYVVVTEGYLTNPRSLWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLVHIAGAYLLNLSRLGLILLLLQYSTEFLFHTARLFYFADENNEKLFSAWAAVFGVTRLFILTLAVLAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVCAAQAWLMWRFIHSQLRHWREYWNEQSAKRRVPATPRLPARLIKRESGYHENGVVKAENGTSPRTKKLKSP

Target ORF information:

RefSeq Version	NM_012288.4
Organism	Homo sapiens(human)
Definition	Homo sapiens translocation associated membrane protein 2 (TRAM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012288.4

ORF Insert Sequence:

ATGGCTTTCC GCAGGAGGAC GAAAAGTTAC CCGCTCTTCA GCCAGGAGTT CGTCATCCAC 
AACCATGCGG ACATCGGCTT CTGCCTGGTG CTCTGCGTCC TCATCGGGCT TATGTTCGAG 
GTCACAGCCA AGACTGCCTT TCTATTTATT TTACCTCAGT ATAACATTAG CGTGCCTACA 
GCAGACAGTG AGACCGTGCA CTACCACTAT GGCCCTAAGG ACCTGGTCAC AATCTTGTTC 
TACATCTTCA TCACCATCAT CTTGCATGCT GTGGTTCAGG AGTACATTTT AGATAAAATC 
AGCAAACGGC TTCATCTCTC CAAAGTCAAA CACAGCAAGT TCAATGAATC TGGACAGCTG 
GTCGTCTTTC ATTTCACCTC GGTGATTTGG TGCTTCTACG TGGTGGTGAC GGAAGGATAC 
TTAACAAACC CAAGAAGCCT CTGGGAAGAC TACCCGCATG TGCACCTCCC CTTCCAGGTG 
AAGTTTTTCT ACCTATGCCA GCTGGCCTAC TGGCTGCACG CACTTCCTGA GCTATACTTC 
CAGAAGGTAC GGAAGGAGGA AATTCCCCGC CAGCTCCAGT ATATTTGCCT GTACCTGGTG 
CATATAGCTG GAGCATACCT CTTAAACCTG AGCCGCCTGG GCCTGATCTT GCTGCTGCTG 
CAGTACTCAA CTGAGTTCCT CTTCCACACG GCTAGACTCT TCTACTTTGC AGATGAAAAC 
AACGAGAAAC TGTTCAGTGC CTGGGCTGCT GTTTTTGGGG TTACCCGCCT CTTCATCCTC 
ACCCTTGCCG TGCTGGCCAT TGGCTTTGGA CTGGCTCGCA TGGAAAACCA GGCATTTGAT 
CCCGAGAAAG GGAACTTCAA CACTTTGTTT TGCAGGCTCT GCGTGCTGCT GCTGGTGTGT 
GCCGCCCAGG CCTGGCTCAT GTGGCGCTTC ATCCACTCCC AGCTGCGGCA CTGGCGGGAA 
TACTGGAATG AGCAGAGTGC AAAGCGGAGA GTCCCAGCCA CACCCAGACT ACCAGCCAGG 
CTCATCAAGA GGGAATCTGG TTACCATGAA AATGGAGTGG TGAAGGCAGA GAACGGAACC 
TCCCCACGGA CTAAGAAACT CAAGTCTCCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRAM2 ( XM_011515005.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513307.1 Homo sapiens translocation associated membrane protein 2 (TRAM2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu63170
Clone ID Related Accession (Same CDS sequence)	XM_011515005.2
Accession Version	XM_011515005.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	translocating chain-associated membrane protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515005.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACAAAT ATTTTATTGA AATCTGGCCT TCAATTACCC CAACACTCAG AAGTGGAATT 
ACTGAAATAT GTGACAGTGA GACCGTGCAC TACCACTATG GCCCTAAGGA CCTGGTCACA 
ATCTTGTTCT ACATCTTCAT CACCATCATC TTGCATGCTG TGGTTCAGGA GTACATTTTA 
GATAAAATCA GCAAACGGCT TCATCTCTCC AAAGTCAAAC ACAGCAAGTT CAATGAATCT 
GGACAGCTGG TCGTCTTTCA TTTCACCTCG GTGATTTGGT GCTTCTACGT GGTGGTGACG 
GAAGGATACT TAACAAACCC AAGAAGCCTC TGGGAAGACT ACCCGCATGT GCACCTCCCC 
TTCCAGGTGA AGTTTTTCTA CCTATGCCAG CTGGCCTACT GGCTGCACGC ACTTCCTGAG 
CTATACTTCC AGAAGGTACG GAAGGAGGAA ATTCCCCGCC AGCTCCAGTA TATTTGCCTG 
TACCTGGTGC ATATAGCTGG AGCATACCTC TTAAACCTGA GCCGCCTGGG CCTGATCTTG 
CTGCTGCTGC AGTACTCAAC TGAGTTCCTC TTCCACACGG CTAGACTCTT CTACTTTGCA 
GATGAAAACA ACGAGAAACT GTTCAGTGCC TGGGCTGCTG TTTTTGGGGT TACCCGCCTC 
TTCATCCTCA CCCTTGCCGT GCTGGCCATT GGCTTTGGAC TGGCTCGCAT GGAAAACCAG 
GCATTTGATC CCGAGAAAGG GAACTTCAAC ACTTTGTTTT GCAGGCTCTG CGTGCTGCTG 
CTGGTGTGTG CCGCCCAGGC CTGGCTCATG TGGCGCTTCA TCCACTCCCA GCTGCGGCAC 
TGGCGGGAAT ACTGGAATGA GCAGAGTGCA AAGCGGAGAG TCCCAGCCAC ACCCAGACTA 
CCAGCCAGGC TCATCAAGAG GGAATCTGGT TACCATGAAA ATGGAGTGGT GAAGGCAGAG 
AACGGAACCT CCCCACGGAC TAAGAAACTC AAGTCTCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513307.1
CDS	136..1137
Translation	MYKYFIEIWPSITPTLRSGITEICDSETVHYHYGPKDLVTILFYIFITIILHAVVQEYILDKISKRLHLSKVKHSKFNESGQLVVFHFTSVIWCFYVVVTEGYLTNPRSLWEDYPHVHLPFQVKFFYLCQLAYWLHALPELYFQKVRKEEIPRQLQYICLYLVHIAGAYLLNLSRLGLILLLLQYSTEFLFHTARLFYFADENNEKLFSAWAAVFGVTRLFILTLAVLAIGFGLARMENQAFDPEKGNFNTLFCRLCVLLLVCAAQAWLMWRFIHSQLRHWREYWNEQSAKRRVPATPRLPARLIKRESGYHENGVVKAENGTSPRTKKLKSP

Target ORF information:

RefSeq Version	XM_011515005.2
Organism	Homo sapiens(human)
Definition	Homo sapiens translocation associated membrane protein 2 (TRAM2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515005.2

ORF Insert Sequence:

ATGTACAAAT ATTTTATTGA AATCTGGCCT TCAATTACCC CAACACTCAG AAGTGGAATT 
ACTGAAATAT GTGACAGTGA GACCGTGCAC TACCACTATG GCCCTAAGGA CCTGGTCACA 
ATCTTGTTCT ACATCTTCAT CACCATCATC TTGCATGCTG TGGTTCAGGA GTACATTTTA 
GATAAAATCA GCAAACGGCT TCATCTCTCC AAAGTCAAAC ACAGCAAGTT CAATGAATCT 
GGACAGCTGG TCGTCTTTCA TTTCACCTCG GTGATTTGGT GCTTCTACGT GGTGGTGACG 
GAAGGATACT TAACAAACCC AAGAAGCCTC TGGGAAGACT ACCCGCATGT GCACCTCCCC 
TTCCAGGTGA AGTTTTTCTA CCTATGCCAG CTGGCCTACT GGCTGCACGC ACTTCCTGAG 
CTATACTTCC AGAAGGTACG GAAGGAGGAA ATTCCCCGCC AGCTCCAGTA TATTTGCCTG 
TACCTGGTGC ATATAGCTGG AGCATACCTC TTAAACCTGA GCCGCCTGGG CCTGATCTTG 
CTGCTGCTGC AGTACTCAAC TGAGTTCCTC TTCCACACGG CTAGACTCTT CTACTTTGCA 
GATGAAAACA ACGAGAAACT GTTCAGTGCC TGGGCTGCTG TTTTTGGGGT TACCCGCCTC 
TTCATCCTCA CCCTTGCCGT GCTGGCCATT GGCTTTGGAC TGGCTCGCAT GGAAAACCAG 
GCATTTGATC CCGAGAAAGG GAACTTCAAC ACTTTGTTTT GCAGGCTCTG CGTGCTGCTG 
CTGGTGTGTG CCGCCCAGGC CTGGCTCATG TGGCGCTTCA TCCACTCCCA GCTGCGGCAC 
TGGCGGGAAT ACTGGAATGA GCAGAGTGCA AAGCGGAGAG TCCCAGCCAC ACCCAGACTA 
CCAGCCAGGC TCATCAAGAG GGAATCTGGT TACCATGAAA ATGGAGTGGT GAAGGCAGAG 
AACGGAACCT CCCCACGGAC TAAGAAACTC AAGTCTCCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inverting the Topology of a Transmembrane Protein by Regulating the Translocation of the First Transmembrane Helix.
Molecular cell63(4)567-578(2016 Aug)
Chen Q,Denard B,Lee CE,Han S,Ye JS,Ye J

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

DAP12 Stabilizes the C-terminal Fragment of the Triggering Receptor Expressed on Myeloid Cells-2 (TREM2) and Protects against LPS-induced Pro-inflammatory Response.
The Journal of biological chemistry290(25)15866-77(2015 Jun)
Zhong L,Chen XF,Zhang ZL,Wang Z,Shi XZ,Xu K,Zhang YW,Xu H,Bu G

Full-length transcriptome analysis of human retina-derived cell lines ARPE-19 and Y79 using the vector-capping method.
Investigative ophthalmology & visual science52(9)6662-70(2011 Aug)
Oshikawa M,Tsutsui C,Ikegami T,Fuchida Y,Matsubara M,Toyama S,Usami R,Ohtoko K,Kato S

Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling.
Gut58(8)1078-83(2009 Aug)
Trynka G,Zhernakova A,Romanos J,Franke L,Hunt KA,Turner G,Bruinenberg M,Heap GA,Platteel M,Ryan AW,de Kovel C,Holmes GK,Howdle PD,Walters JR,Sanders DS,Mulder CJ,Mearin ML,Verbeek WH,Trimble V,Stevens FM,Kelleher D,Barisani D,Bardella MT,McManus R,van Heel DA,Wijmenga C

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TRAM2 cDNA ORF clone, Homo sapiens(human)

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ORF Insert Sequence:

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ORF Insert Sequence:

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