CEP135 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CEP135
Entrez Gene ID	9662
Full Name	centrosomal protein 135
Synonyms	CEP4,KIAA0635,MCPH8
General protein information	Preferred Names centrosomal protein 135 Names centrosomal protein of 135 kDa centrosomal protein 135kDa centrosomal protein 4 centrosome protein cep135
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q12
Summary	This gene encodes a centrosomal protein, which acts as a scaffolding protein during early centriole biogenesis, and is also required for centriole-centriole cohesion during interphase. Mutations in this gene are associated with autosomal recessive primary microcephaly-8. [provided by RefSeq, Jun 2012].
Disorder MIM:	611423
Disorder Html:	Microcephaly 8, primary, autosomal recessive, 614673 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005265788.3	XP_005265845.1	centrosomal protein of 135 kDa isoform X2
XM_006714055.2	XP_006714118.1	centrosomal protein of 135 kDa isoform X1
XM_005265788.4	XP_005265845.1	centrosomal protein of 135 kDa isoform X2
NM_025009.4	NP_079285.2	centrosomal protein of 135 kDa
XM_006714055.3	XP_006714118.1	centrosomal protein of 135 kDa isoform X1
XM_011534412.2	XP_011532714.1	centrosomal protein of 135 kDa isoform X3
NM_025009.5	NP_079285.2	centrosomal protein of 135 kDa

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Cep135	NP_001189108.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010985	XP_309709.4
Bos taurus (cattle)	CEP135	NP_001068874.2
Xenopus tropicalis (tropical clawed frog)	cep135	XP_002934717.1
Homo sapiens (human)	CEP135	NP_079285.2
	CEP135	XP_001084949.2
Rattus norvegicus (Norway rat)	Cep135	XP_223341.6
Gallus gallus (chicken)	CEP135	XP_004936077.1
Danio rerio (zebrafish)	cep135	NP_001186919.1
Pan troglodytes (chimpanzee)	CEP135	XP_517281.2
Canis lupus familiaris (dog)	CEP135	XP_539276.3
Mus musculus (house mouse)	Cep135	NP_950197.1

Gene Ontology
Bibliography

Related articles in PubMed

The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding.
Kraatz S, Guichard P, Obbineni JM, Olieric N, Hatzopoulos GN, Hilbert M, Sen I, Missimer J, G?nczy P, Steinmetz MO
Structure (London, England : 1993)24(8)1358-1371(2016 Aug)

CEP295 interacts with microtubules and is required for centriole elongation.
Chang CW, Hsu WB, Tsai JJ, Tang CJ, Tang TK
Journal of cell science129(13)2501-13(2016 07)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identified the second mutation in CEP135, confirming the role during embryonic brain development and in the pathophysiology of human primary microcephaly
Title: A novel splice site mutation in CEP135 is associated with primary microcephaly in a Pakistani family.

Authors suggest that the decrease in CEP135(mini) from centrosomes promotes centriole assembly. The repression of centriole duplication by a splice isoform of a protein that normally promotes it serves as a novel mechanism to limit centriole duplication.
Title: A Short CEP135 Splice Isoform Controls Centriole Duplication.

These findings support a model in which PKC-mediated phosphorylation of CEP4 at Ser(18) and Ser(80) causes its dissociation from Cdc42, thereby increasing its affinity for TEM4 and producing Rac activation, filopodium formation, and cell motility
Title: Phosphorylation of Cdc42 effector protein-4 (CEP4) by protein kinase C promotes motility of human breast cells.

Authors propose that CEP135 directly connects the central hub protein, hSAS-6, to the outer microtubules, and suggest that this interaction stabilizes the proper cartwheel structure for further CPAP-mediated centriole elongation.
Title: Human microcephaly protein CEP135 binds to hSAS-6 and CPAP, and is required for centriole assembly.

A truncating mutation of CEP135 caused autosomal-recessive primary microcephaly in a Pakistani family.
Title: A truncating mutation of CEP135 causes primary microcephaly and disturbed centrosomal function.

The following CEP135 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEP135 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20292	NM_025009.5 Latest version!	Homo sapiens centrosomal protein 135 (CEP135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu63113	XM_011534412.2 Latest version!	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu38651	XM_005265788.4 Latest version!	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38650	XM_006714055.3 Latest version!	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu38651	XM_005265788.3	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu38650	XM_006714055.2	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu20292	NM_025009.4	Homo sapiens centrosomal protein 135 (CEP135), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CEP135 ( NM_025009.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_079285.2 Homo sapiens centrosomal protein 135 (CEP135), mRNA.

Price & Availability↑

CloneID	OHu20292
Clone ID Related Accession (Same CDS sequence)	NM_025009.4 , NM_025009.5
Accession Version	NM_025009.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BK005586.1, BC136535.1, CA438857.1 and BU734129.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_025009.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.172259.1, BK005586.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000257287.5/ ENSP00000257287.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGAAAGAG ATTAAAAGAA AGCTCTCTGA AATGCAGGAT 
CTTGAAGAAA CAATGGCAAA ACTTCAGCTG GAATTGAACT TATGCCAGAA AGAAAAGGAG 
AGACTGAGTG ATGAACTCCT TGTAAAATCA GACCTAGAAA CTGTTGTTCA TCAGCTTGAA 
CAAGAAAAGC AAAGACTTAG CAAAAAAGTT GAAAGTTTTG CAGTTACAGA ACGACAACTT 
ACTCTGGAGG TTGAGAGGAT GAGACTAGAA CATGGAATAA AACGTCGAGA CAGGTCACCT 
TCTCGTTTAG ATACATTTCT GAAAGGTATA GAAGAAGAAC GAGATTATTA TAAGAAAGAG 
CTAGAGAGAC TCCAACATAT AATACAGCGA AGATCTTGCT CTACAAGTTA TAGCGCACGT 
GAAAAAAGTT CAATATTTAG AACACCAGAA AAGGGTGATT ACAATTCAGA AATTCATCAG 
ATCACAAGAG AAAGAGATGA ACTTCAGCGT ATGCTAGAAA GATTTGAAAA ATATATGGAA 
GATATACAGT CCAATGTTAA ATTATTGACA GCAGAAAGAG ATAAACTAAG TGTCTTATAT 
AATGAAGCTC AGGAAGAATT ATCTGCCCTA AGAAAGGAAT CCACCCAAAC CACAGCACCC 
CATAATATTG TTAGTCTTAT GGAAAAGGAA AAAGAACTTG CGTTATCTGA CTTAAGAAGA 
ATTATGGCAG AAAAGGAAGC TTTAAGAGAA AAATTAGAGC ATATTGAAGA AGTGAGTCTT 
TTTGGAAAAT CAGAATTAGA GAAAACTATT GAACATTTGA CATGTGTTAA TCATCAGCTT 
GAAAGCGAAA AATATGAATT AAAGTCTAAA GTGTTAATAA TGAAAGAAAC AATAGAGTCG 
TTAGAGAACA AATTAAAAGT CCAAGCTCAA AAATTTAGCC ATGTGGCTGG TGACTCATCT 
CATCAGAAAA CAGAGGTGAA CTCACTTAGG ATAGTGAATG AGCAGCTACA GCGGTCAGTT 
GATGACTATC AGCACCGACT TTCCATAAAA AGAGGTGAAC TTGAATCAGC CCAAGCACAA 
ATTAAAATAC TGGAGGAAAA GATAGATGAA CTAAACCTTA AGATGACTTC ACAGGATGAG 
GAGGCTCATG TAATGAAAAA GACCATTGGT GTTATTGATA AAGAAAAAGA CTTTCTCCAG 
GAGACTGTAG ATGAGAAGAC AGAAAAGATT GCAAATTTGC AAGAAAACCT AGCTAATAAA 
GAAAAAGCTG TTGCTCAAAT GAAGATAATG ATCTCAGAGT GTGAATCATC TGTGAACCAG 
CTGAAAGAAA CATTGGTTAA TCGAGATCGT GAGATAAACA GCCTCCGGCG CCAGCTTGAT 
GCAGCTCACA AAGAACTCGA TGAAGTAGGA AGATCTAGAG AAATCGCTTT TAAGGAAAAC 
AGAAGACTGC AAGATGACCT GGCTACAATG GCAAGAGAAA ATCAAGAAAT CTCATTGGAA 
TTGGAAGCAG CAGTGCAAGA AAAAGAAGAA ATGAAGAGCA GAGTTCATAA ATACATAACA 
GAGGTGTCAC GATGGGAGAG CTTAATGGCT GCCAAGGAAA AAGAAAATCA AGATTTGTTA 
GATAGATTTC AGATGCTTCA TAACCGTGCT GAAGACTGGG AGGTCAAAGC CCATCAAGCT 
GAGGGAGAAA GCAGCTCAGT TCGACTGGAA CTTCTTTCTA TTGACACTGA GAGGAGACAT 
CTTCGAGAAA GAGTGGAGCT ATTAGAAAAA GAAATTCAAG AGCACATAAA TGCCCATCAT 
GCTTATGAAT CTCAGATCTC ATCAATGGCA AAAGCCATGT CTCGATTAGA AGAAGAGCTG 
AGACATCAAG AAGATGAGAA AGCAACAGTA TTAAATGACT TGTCATCTCT TAGAGAACTT 
TGCATTAAAC TTGATTCAGG CAAAGATATT ATGACCCAGC AATTGAATTC GAAAAACCTT 
GAGTTTGAGA GGGTTGTGGT GGAATTAGAA AATGTAAAGT CAGAGTCAGA CCTACTGAAA 
AAACAACTTT CAAATGAGAG ACATACAGTT AAAAACCTCG AATCATTGTT GGCTACAAAC 
AGAGATAAAG AATTTCATTC TCACTTAACC TCCCACGAGA AGGATACAGA AATCCAGCTA 
CTTAAGGAGA AGTTAACCCT TTCTGAAAGC AAATTAACTA GTCAAAGCCG GGAAAACACC 
ATGCTTCGAG CTAAAGTGGC ACAGTTACAA ACAGATTATG ATGCTCTGAA AAGGCAGATC 
TCAACTGAAA GATACGAACG AGAACGAGCA ATCCAAGAGA TGCGTCGACA TGGTCTTGCT 
ACACCACCCC TTAGTTCCAC TCTGAGGTCT CCTTCACATT CTCCTGAACA TAGAAATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079285.2
CDS	161..3583
Translation	MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	NM_025009.5
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025009.5

ORF Insert Sequence:

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGAAAGAG ATTAAAAGAA AGCTCTCTGA AATGCAGGAT 
CTTGAAGAAA CAATGGCAAA ACTTCAGCTG GAATTGAACT TATGCCAGAA AGAAAAGGAG 
AGACTGAGTG ATGAACTCCT TGTAAAATCA GACCTAGAAA CTGTTGTTCA TCAGCTTGAA 
CAAGAAAAGC AAAGACTTAG CAAAAAAGTT GAAAGTTTTG CAGTTACAGA ACGACAACTT 
ACTCTGGAGG TTGAGAGGAT GAGACTAGAA CATGGAATAA AACGTCGAGA CAGGTCACCT 
TCTCGTTTAG ATACATTTCT GAAAGGTATA GAAGAAGAAC GAGATTATTA TAAGAAAGAG 
CTAGAGAGAC TCCAACATAT AATACAGCGA AGATCTTGCT CTACAAGTTA TAGCGCACGT 
GAAAAAAGTT CAATATTTAG AACACCAGAA AAGGGTGATT ACAATTCAGA AATTCATCAG 
ATCACAAGAG AAAGAGATGA ACTTCAGCGT ATGCTAGAAA GATTTGAAAA ATATATGGAA 
GATATACAGT CCAATGTTAA ATTATTGACA GCAGAAAGAG ATAAACTAAG TGTCTTATAT 
AATGAAGCTC AGGAAGAATT ATCTGCCCTA AGAAAGGAAT CCACCCAAAC CACAGCACCC 
CATAATATTG TTAGTCTTAT GGAAAAGGAA AAAGAACTTG CGTTATCTGA CTTAAGAAGA 
ATTATGGCAG AAAAGGAAGC TTTAAGAGAA AAATTAGAGC ATATTGAAGA AGTGAGTCTT 
TTTGGAAAAT CAGAATTAGA GAAAACTATT GAACATTTGA CATGTGTTAA TCATCAGCTT 
GAAAGCGAAA AATATGAATT AAAGTCTAAA GTGTTAATAA TGAAAGAAAC AATAGAGTCG 
TTAGAGAACA AATTAAAAGT CCAAGCTCAA AAATTTAGCC ATGTGGCTGG TGACTCATCT 
CATCAGAAAA CAGAGGTGAA CTCACTTAGG ATAGTGAATG AGCAGCTACA GCGGTCAGTT 
GATGACTATC AGCACCGACT TTCCATAAAA AGAGGTGAAC TTGAATCAGC CCAAGCACAA 
ATTAAAATAC TGGAGGAAAA GATAGATGAA CTAAACCTTA AGATGACTTC ACAGGATGAG 
GAGGCTCATG TAATGAAAAA GACCATTGGT GTTATTGATA AAGAAAAAGA CTTTCTCCAG 
GAGACTGTAG ATGAGAAGAC AGAAAAGATT GCAAATTTGC AAGAAAACCT AGCTAATAAA 
GAAAAAGCTG TTGCTCAAAT GAAGATAATG ATCTCAGAGT GTGAATCATC TGTGAACCAG 
CTGAAAGAAA CATTGGTTAA TCGAGATCGT GAGATAAACA GCCTCCGGCG CCAGCTTGAT 
GCAGCTCACA AAGAACTCGA TGAAGTAGGA AGATCTAGAG AAATCGCTTT TAAGGAAAAC 
AGAAGACTGC AAGATGACCT GGCTACAATG GCAAGAGAAA ATCAAGAAAT CTCATTGGAA 
TTGGAAGCAG CAGTGCAAGA AAAAGAAGAA ATGAAGAGCA GAGTTCATAA ATACATAACA 
GAGGTGTCAC GATGGGAGAG CTTAATGGCT GCCAAGGAAA AAGAAAATCA AGATTTGTTA 
GATAGATTTC AGATGCTTCA TAACCGTGCT GAAGACTGGG AGGTCAAAGC CCATCAAGCT 
GAGGGAGAAA GCAGCTCAGT TCGACTGGAA CTTCTTTCTA TTGACACTGA GAGGAGACAT 
CTTCGAGAAA GAGTGGAGCT ATTAGAAAAA GAAATTCAAG AGCACATAAA TGCCCATCAT 
GCTTATGAAT CTCAGATCTC ATCAATGGCA AAAGCCATGT CTCGATTAGA AGAAGAGCTG 
AGACATCAAG AAGATGAGAA AGCAACAGTA TTAAATGACT TGTCATCTCT TAGAGAACTT 
TGCATTAAAC TTGATTCAGG CAAAGATATT ATGACCCAGC AATTGAATTC GAAAAACCTT 
GAGTTTGAGA GGGTTGTGGT GGAATTAGAA AATGTAAAGT CAGAGTCAGA CCTACTGAAA 
AAACAACTTT CAAATGAGAG ACATACAGTT AAAAACCTCG AATCATTGTT GGCTACAAAC 
AGAGATAAAG AATTTCATTC TCACTTAACC TCCCACGAGA AGGATACAGA AATCCAGCTA 
CTTAAGGAGA AGTTAACCCT TTCTGAAAGC AAATTAACTA GTCAAAGCCG GGAAAACACC 
ATGCTTCGAG CTAAAGTGGC ACAGTTACAA ACAGATTATG ATGCTCTGAA AAGGCAGATC 
TCAACTGAAA GATACGAACG AGAACGAGCA ATCCAAGAGA TGCGTCGACA TGGTCTTGCT 
ACACCACCCC TTAGTTCCAC TCTGAGGTCT CCTTCACATT CTCCTGAACA TAGAAATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP135 ( XM_011534412.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532714.1 Homo sapiens centrosomal protein 135 (CEP135), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu63113
Clone ID Related Accession (Same CDS sequence)	XM_011534412.2
Accession Version	XM_011534412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011534412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGAAA GATTTGAAAA ATATATGGAA GATATACAGT CCAATGTTAA ATTATTGACA 
GCAGAAAGAG ATAAACTAAG TGTCTTATAT AATGAAGCTC AGGAAGAATT ATCTGCCCTA 
AGAAAGGAAT CCACCCAAAC CACAGCACCC CATAATATTG TTAGTCTTAT GGAAAAGGAA 
AAAGAACTTG CGTTATCTGA CTTAAGAAGA ATTATGGCAG AAAAGGAAGC TTTAAGAGAA 
AAATTAGAGC ATATTGAAGA AGTGAGTCTT TTTGGAAAAT CAGAATTAGA GAAAACTATT 
GAACATTTGA CATGTGTTAA TCATCAGCTT GAAAGCGAAA AATATGAATT AAAGTCTAAA 
GTGTTAATAA TGAAAGAAAC AATAGAGTCG TTAGAGAACA AATTAAAAGT CCAAGCTCAA 
AAATTTAGCC ATGTGGCTGG TGACTCATCT CATCAGAAAA CAGAGGTGAA CTCACTTAGG 
ATAGTGAATG AGCAGCTACA GCGGTCAGTT GATGACTATC AGCACCGACT TTCCATAAAA 
AGAGGTGAAC TTGAATCAGC CCAAGCACAA ATTAAAATAC TGGAGGAAAA GATAGATGAA 
CTAAACCTTA AGATGACTTC ACAGGATGAG GAGGCTCATG TAATGAAAAA GACCATTGGT 
GTTATTGATA AAGAAAAAGA CTTTCTCCAG GAGACTGTAG ATGAGAAGAC AGAAAAGATT 
GCAAATTTGC AAGAAAACCT AGCTAATAAA GAAAAAGCTG TTGCTCAAAT GAAGATAATG 
ATCTCAGAGT GTGAATCATC TGTGAACCAG CTGAAAGAAA CATTGGTTAA TCGAGATCGT 
GAGATAAACA GCCTCCGGCG CCAGCTTGAT GCAGCTCACA AAGAACTCGA TGAAGTAGGA 
AGATCTAGAG AAATCGCTTT TAAGGAAAAC AGAAGACTGC AAGATGACCT GGCTACAATG 
GCAAGAGAAA ATCAAGAAAT CTCATTGGAA TTGGAAGCAG CAGTGCAAGA AAAAGAAGAA 
ATGAAGAGCA GAGTTCATAA ATACATAACA GAGGTGTCAC GATGGGAGAG CTTAATGGCT 
GCCAAGGAAA AAGAAAATCA AGATTTGTTA GATAGATTTC AGATGCTTCA TAACCGTGCT 
GAAGACTGGG AGGTCAAAGC CCATCAAGCT GAGGGAGAAA GCAGCTCAGT TCGACTGGAA 
CTTCTTTCTA TTGACACTGA GAGGAGACAT CTTCGAGAAA GAGTGGAGCT ATTAGAAAAA 
GAAATTCAAG AGCACATAAA TGCCCATCAT GCTTATGAAT CTCAGATCTC ATCAATGGCA 
AAAGCCATGT CTCGATTAGA AGAAGAGCTG AGACATCAAG AAGATGAGAA AGCAACAGTA 
TTAAATGACT TGTCATCTCT TAGAGAACTT TGCATTAAAC TTGATTCAGG CAAAGATATT 
ATGACCCAGC AATTGAATTC GAAAAACCTT GAGTTTGAGA GGGTTGTGGT GGAATTAGAA 
AATGTAAAGT CAGAGTCAGA CCTACTGAAA AAACAACTTT CAAATGAGAG ACATACAGTT 
AAAAACCTCG AATCATTGTT GGCTACAAAC AGAGATAAAG AATTTCATTC TCACTTAACC 
TCCCACGAGA AGGATACAGA AATCCAGCTA CTTAAGGAGA AGTTAACCCT TTCTGAAAGC 
AAATTAACTA GTCAAAGCCG GGAAAACACC ATGCTTCGAG CTAAAGTGGC ACAGTTACAA 
ACAGATTATG ATGCTCTGAA AAGGCAGATC TCAACTGAAA GATACGAACG AGAACGAGCA 
ATCCAAGAGA TGCGTCGACA TGGTCTTGCT ACACCACCCC TTAGTTCCAC TCTGAGGTCT 
CCTTCACATT CTCCTGAACA TAGAAATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532714.1
CDS	147..2039
Translation	MLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	XM_011534412.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011534412.2

ORF Insert Sequence:

ATGCTAGAAA GATTTGAAAA ATATATGGAA GATATACAGT CCAATGTTAA ATTATTGACA 
GCAGAAAGAG ATAAACTAAG TGTCTTATAT AATGAAGCTC AGGAAGAATT ATCTGCCCTA 
AGAAAGGAAT CCACCCAAAC CACAGCACCC CATAATATTG TTAGTCTTAT GGAAAAGGAA 
AAAGAACTTG CGTTATCTGA CTTAAGAAGA ATTATGGCAG AAAAGGAAGC TTTAAGAGAA 
AAATTAGAGC ATATTGAAGA AGTGAGTCTT TTTGGAAAAT CAGAATTAGA GAAAACTATT 
GAACATTTGA CATGTGTTAA TCATCAGCTT GAAAGCGAAA AATATGAATT AAAGTCTAAA 
GTGTTAATAA TGAAAGAAAC AATAGAGTCG TTAGAGAACA AATTAAAAGT CCAAGCTCAA 
AAATTTAGCC ATGTGGCTGG TGACTCATCT CATCAGAAAA CAGAGGTGAA CTCACTTAGG 
ATAGTGAATG AGCAGCTACA GCGGTCAGTT GATGACTATC AGCACCGACT TTCCATAAAA 
AGAGGTGAAC TTGAATCAGC CCAAGCACAA ATTAAAATAC TGGAGGAAAA GATAGATGAA 
CTAAACCTTA AGATGACTTC ACAGGATGAG GAGGCTCATG TAATGAAAAA GACCATTGGT 
GTTATTGATA AAGAAAAAGA CTTTCTCCAG GAGACTGTAG ATGAGAAGAC AGAAAAGATT 
GCAAATTTGC AAGAAAACCT AGCTAATAAA GAAAAAGCTG TTGCTCAAAT GAAGATAATG 
ATCTCAGAGT GTGAATCATC TGTGAACCAG CTGAAAGAAA CATTGGTTAA TCGAGATCGT 
GAGATAAACA GCCTCCGGCG CCAGCTTGAT GCAGCTCACA AAGAACTCGA TGAAGTAGGA 
AGATCTAGAG AAATCGCTTT TAAGGAAAAC AGAAGACTGC AAGATGACCT GGCTACAATG 
GCAAGAGAAA ATCAAGAAAT CTCATTGGAA TTGGAAGCAG CAGTGCAAGA AAAAGAAGAA 
ATGAAGAGCA GAGTTCATAA ATACATAACA GAGGTGTCAC GATGGGAGAG CTTAATGGCT 
GCCAAGGAAA AAGAAAATCA AGATTTGTTA GATAGATTTC AGATGCTTCA TAACCGTGCT 
GAAGACTGGG AGGTCAAAGC CCATCAAGCT GAGGGAGAAA GCAGCTCAGT TCGACTGGAA 
CTTCTTTCTA TTGACACTGA GAGGAGACAT CTTCGAGAAA GAGTGGAGCT ATTAGAAAAA 
GAAATTCAAG AGCACATAAA TGCCCATCAT GCTTATGAAT CTCAGATCTC ATCAATGGCA 
AAAGCCATGT CTCGATTAGA AGAAGAGCTG AGACATCAAG AAGATGAGAA AGCAACAGTA 
TTAAATGACT TGTCATCTCT TAGAGAACTT TGCATTAAAC TTGATTCAGG CAAAGATATT 
ATGACCCAGC AATTGAATTC GAAAAACCTT GAGTTTGAGA GGGTTGTGGT GGAATTAGAA 
AATGTAAAGT CAGAGTCAGA CCTACTGAAA AAACAACTTT CAAATGAGAG ACATACAGTT 
AAAAACCTCG AATCATTGTT GGCTACAAAC AGAGATAAAG AATTTCATTC TCACTTAACC 
TCCCACGAGA AGGATACAGA AATCCAGCTA CTTAAGGAGA AGTTAACCCT TTCTGAAAGC 
AAATTAACTA GTCAAAGCCG GGAAAACACC ATGCTTCGAG CTAAAGTGGC ACAGTTACAA 
ACAGATTATG ATGCTCTGAA AAGGCAGATC TCAACTGAAA GATACGAACG AGAACGAGCA 
ATCCAAGAGA TGCGTCGACA TGGTCTTGCT ACACCACCCC TTAGTTCCAC TCTGAGGTCT 
CCTTCACATT CTCCTGAACA TAGAAATGTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP135 ( XM_005265788.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265845.1 Homo sapiens centrosomal protein 135 (CEP135), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu38651
Clone ID Related Accession (Same CDS sequence)	XM_005265788.3 , XM_005265788.4
Accession Version	XM_005265788.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005265788.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC TTGAAGAAAC AATGGCAAAA CTTCAGCTGG AATTGAACTT ATGCCAGAAA 
GAAAAGGAGA GACTGAGTGA TGAACTCCTT GTAAAATCAG ACCTAGAAAC TGTTGTTCAT 
CAGCTTGAAC AAGAAAAGCA AAGACTTAGC AAAAAAGTTG AAAGTTTTGC AGTTACAGAA 
CGACAACTTA CTCTGGAGGT TGAGAGGATG AGACTAGAAC ATGGAATAAA ACGTCGAGAC 
AGGTCACCTT CTCGTTTAGA TACATTTCTG AAAGGTATAG AAGAAGAACG AGATTATTAT 
AAGAAAGAGC TAGAGAGACT CCAACATATA ATACAGCGAA GATCTTGCTC TACAAGTTAT 
AGCGCACGTG AAAAAAGTTC AATATTTAGA ACACCAGAAA AGGGTGATTA CAATTCAGAA 
ATTCATCAGA TCACAAGAGA AAGAGATGAA CTTCAGCGTA TGCTAGAAAG ATTTGAAAAA 
TATATGGAAG ATATACAGTC CAATGTTAAA TTATTGACAG CAGAAAGAGA TAAACTAAGT 
GTCTTATATA ATGAAGCTCA GGAAGAATTA TCTGCCCTAA GAAAGGAATC CACCCAAACC 
ACAGCACCCC ATAATATTGT TAGTCTTATG GAAAAGGAAA AAGAACTTGC GTTATCTGAC 
TTAAGAAGAA TTATGGCAGA AAAGGAAGCT TTAAGAGAAA AATTAGAGCA TATTGAAGAA 
GTGAGTCTTT TTGGAAAATC AGAATTAGAG AAAACTATTG AACATTTGAC ATGTGTTAAT 
CATCAGCTTG AAAGCGAAAA ATATGAATTA AAGTCTAAAG TGTTAATAAT GAAAGAAACA 
ATAGAGTCGT TAGAGAACAA ATTAAAAGTC CAAGCTCAAA AATTTAGCCA TGTGGCTGGT 
GACTCATCTC ATCAGAAAAC AGAGGTGAAC TCACTTAGGA TAGTGAATGA GCAGCTACAG 
CGGTCAGTTG ATGACTATCA GCACCGACTT TCCATAAAAA GAGGTGAACT TGAATCAGCC 
CAAGCACAAA TTAAAATACT GGAGGAAAAG ATAGATGAAC TAAACCTTAA GATGACTTCA 
CAGGATGAGG AGGCTCATGT AATGAAAAAG ACCATTGGTG TTATTGATAA AGAAAAAGAC 
TTTCTCCAGG AGACTGTAGA TGAGAAGACA GAAAAGATTG CAAATTTGCA AGAAAACCTA 
GCTAATAAAG AAAAAGCTGT TGCTCAAATG AAGATAATGA TCTCAGAGTG TGAATCATCT 
GTGAACCAGC TGAAAGAAAC ATTGGTTAAT CGAGATCGTG AGATAAACAG CCTCCGGCGC 
CAGCTTGATG CAGCTCACAA AGAACTCGAT GAAGTAGGAA GATCTAGAGA AATCGCTTTT 
AAGGAAAACA GAAGACTGCA AGATGACCTG GCTACAATGG CAAGAGAAAA TCAAGAAATC 
TCATTGGAAT TGGAAGCAGC AGTGCAAGAA AAAGAAGAAA TGAAGAGCAG AGTTCATAAA 
TACATAACAG AGGTGTCACG ATGGGAGAGC TTAATGGCTG CCAAGGAAAA AGAAAATCAA 
GATTTGTTAG ATAGATTTCA GATGCTTCAT AACCGTGCTG AAGACTGGGA GGTCAAAGCC 
CATCAAGCTG AGGGAGAAAG CAGCTCAGTT CGACTGGAAC TTCTTTCTAT TGACACTGAG 
AGGAGACATC TTCGAGAAAG AGTGGAGCTA TTAGAAAAAG AAATTCAAGA GCACATAAAT 
GCCCATCATG CTTATGAATC TCAGATCTCA TCAATGGCAA AAGCCATGTC TCGATTAGAA 
GAAGAGCTGA GACATCAAGA AGATGAGAAA GCAACAGTAT TAAATGACTT GTCATCTCTT 
AGAGAACTTT GCATTAAACT TGATTCAGGC AAAGATATTA TGACCCAGCA ATTGAATTCG 
AAAAACCTTG AGTTTGAGAG GGTTGTGGTG GAATTAGAAA ATGTAAAGTC AGAGTCAGAC 
CTACTGAAAA AACAACTTTC AAATGAGAGA CATACAGTTA AAAACCTCGA ATCATTGTTG 
GCTACAAACA GAGATAAAGA ATTTCATTCT CACTTAACCT CCCACGAGAA GGATACAGAA 
ATCCAGCTAC TTAAGGAGAA GTTAACCCTT TCTGAAAGCA AATTAACTAG TCAAAGCCGG 
GAAAACACCA TGCTTCGAGC TAAAGTGGCA CAGTTACAAA CAGATTATGA TGCTCTGAAA 
AGGCAGATCT CAACTGAAAG ATACGAACGA GAACGAGCAA TCCAAGAGAT GCGTCGACAT 
GGTCTTGCTA CACCACCCCT TAGTTCCACT CTGAGGTCTC CTTCACATTC TCCTGAACAT 
AGAAATGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265845.1
CDS	1336..3687
Translation	MQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	XM_005265788.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265788.3

ORF Insert Sequence:

ATGCAGGATC TTGAAGAAAC AATGGCAAAA CTTCAGCTGG AATTGAACTT ATGCCAGAAA 
GAAAAGGAGA GACTGAGTGA TGAACTCCTT GTAAAATCAG ACCTAGAAAC TGTTGTTCAT 
CAGCTTGAAC AAGAAAAGCA AAGACTTAGC AAAAAAGTTG AAAGTTTTGC AGTTACAGAA 
CGACAACTTA CTCTGGAGGT TGAGAGGATG AGACTAGAAC ATGGAATAAA ACGTCGAGAC 
AGGTCACCTT CTCGTTTAGA TACATTTCTG AAAGGTATAG AAGAAGAACG AGATTATTAT 
AAGAAAGAGC TAGAGAGACT CCAACATATA ATACAGCGAA GATCTTGCTC TACAAGTTAT 
AGCGCACGTG AAAAAAGTTC AATATTTAGA ACACCAGAAA AGGGTGATTA CAATTCAGAA 
ATTCATCAGA TCACAAGAGA AAGAGATGAA CTTCAGCGTA TGCTAGAAAG ATTTGAAAAA 
TATATGGAAG ATATACAGTC CAATGTTAAA TTATTGACAG CAGAAAGAGA TAAACTAAGT 
GTCTTATATA ATGAAGCTCA GGAAGAATTA TCTGCCCTAA GAAAGGAATC CACCCAAACC 
ACAGCACCCC ATAATATTGT TAGTCTTATG GAAAAGGAAA AAGAACTTGC GTTATCTGAC 
TTAAGAAGAA TTATGGCAGA AAAGGAAGCT TTAAGAGAAA AATTAGAGCA TATTGAAGAA 
GTGAGTCTTT TTGGAAAATC AGAATTAGAG AAAACTATTG AACATTTGAC ATGTGTTAAT 
CATCAGCTTG AAAGCGAAAA ATATGAATTA AAGTCTAAAG TGTTAATAAT GAAAGAAACA 
ATAGAGTCGT TAGAGAACAA ATTAAAAGTC CAAGCTCAAA AATTTAGCCA TGTGGCTGGT 
GACTCATCTC ATCAGAAAAC AGAGGTGAAC TCACTTAGGA TAGTGAATGA GCAGCTACAG 
CGGTCAGTTG ATGACTATCA GCACCGACTT TCCATAAAAA GAGGTGAACT TGAATCAGCC 
CAAGCACAAA TTAAAATACT GGAGGAAAAG ATAGATGAAC TAAACCTTAA GATGACTTCA 
CAGGATGAGG AGGCTCATGT AATGAAAAAG ACCATTGGTG TTATTGATAA AGAAAAAGAC 
TTTCTCCAGG AGACTGTAGA TGAGAAGACA GAAAAGATTG CAAATTTGCA AGAAAACCTA 
GCTAATAAAG AAAAAGCTGT TGCTCAAATG AAGATAATGA TCTCAGAGTG TGAATCATCT 
GTGAACCAGC TGAAAGAAAC ATTGGTTAAT CGAGATCGTG AGATAAACAG CCTCCGGCGC 
CAGCTTGATG CAGCTCACAA AGAACTCGAT GAAGTAGGAA GATCTAGAGA AATCGCTTTT 
AAGGAAAACA GAAGACTGCA AGATGACCTG GCTACAATGG CAAGAGAAAA TCAAGAAATC 
TCATTGGAAT TGGAAGCAGC AGTGCAAGAA AAAGAAGAAA TGAAGAGCAG AGTTCATAAA 
TACATAACAG AGGTGTCACG ATGGGAGAGC TTAATGGCTG CCAAGGAAAA AGAAAATCAA 
GATTTGTTAG ATAGATTTCA GATGCTTCAT AACCGTGCTG AAGACTGGGA GGTCAAAGCC 
CATCAAGCTG AGGGAGAAAG CAGCTCAGTT CGACTGGAAC TTCTTTCTAT TGACACTGAG 
AGGAGACATC TTCGAGAAAG AGTGGAGCTA TTAGAAAAAG AAATTCAAGA GCACATAAAT 
GCCCATCATG CTTATGAATC TCAGATCTCA TCAATGGCAA AAGCCATGTC TCGATTAGAA 
GAAGAGCTGA GACATCAAGA AGATGAGAAA GCAACAGTAT TAAATGACTT GTCATCTCTT 
AGAGAACTTT GCATTAAACT TGATTCAGGC AAAGATATTA TGACCCAGCA ATTGAATTCG 
AAAAACCTTG AGTTTGAGAG GGTTGTGGTG GAATTAGAAA ATGTAAAGTC AGAGTCAGAC 
CTACTGAAAA AACAACTTTC AAATGAGAGA CATACAGTTA AAAACCTCGA ATCATTGTTG 
GCTACAAACA GAGATAAAGA ATTTCATTCT CACTTAACCT CCCACGAGAA GGATACAGAA 
ATCCAGCTAC TTAAGGAGAA GTTAACCCTT TCTGAAAGCA AATTAACTAG TCAAAGCCGG 
GAAAACACCA TGCTTCGAGC TAAAGTGGCA CAGTTACAAA CAGATTATGA TGCTCTGAAA 
AGGCAGATCT CAACTGAAAG ATACGAACGA GAACGAGCAA TCCAAGAGAT GCGTCGACAT 
GGTCTTGCTA CACCACCCCT TAGTTCCACT CTGAGGTCTC CTTCACATTC TCCTGAACAT 
AGAAATGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP135 ( XM_005265788.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005265845.1 Homo sapiens centrosomal protein 135 (CEP135), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu38651
Clone ID Related Accession (Same CDS sequence)	XM_005265788.3 , XM_005265788.4
Accession Version	XM_005265788.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005265788.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGATC TTGAAGAAAC AATGGCAAAA CTTCAGCTGG AATTGAACTT ATGCCAGAAA 
GAAAAGGAGA GACTGAGTGA TGAACTCCTT GTAAAATCAG ACCTAGAAAC TGTTGTTCAT 
CAGCTTGAAC AAGAAAAGCA AAGACTTAGC AAAAAAGTTG AAAGTTTTGC AGTTACAGAA 
CGACAACTTA CTCTGGAGGT TGAGAGGATG AGACTAGAAC ATGGAATAAA ACGTCGAGAC 
AGGTCACCTT CTCGTTTAGA TACATTTCTG AAAGGTATAG AAGAAGAACG AGATTATTAT 
AAGAAAGAGC TAGAGAGACT CCAACATATA ATACAGCGAA GATCTTGCTC TACAAGTTAT 
AGCGCACGTG AAAAAAGTTC AATATTTAGA ACACCAGAAA AGGGTGATTA CAATTCAGAA 
ATTCATCAGA TCACAAGAGA AAGAGATGAA CTTCAGCGTA TGCTAGAAAG ATTTGAAAAA 
TATATGGAAG ATATACAGTC CAATGTTAAA TTATTGACAG CAGAAAGAGA TAAACTAAGT 
GTCTTATATA ATGAAGCTCA GGAAGAATTA TCTGCCCTAA GAAAGGAATC CACCCAAACC 
ACAGCACCCC ATAATATTGT TAGTCTTATG GAAAAGGAAA AAGAACTTGC GTTATCTGAC 
TTAAGAAGAA TTATGGCAGA AAAGGAAGCT TTAAGAGAAA AATTAGAGCA TATTGAAGAA 
GTGAGTCTTT TTGGAAAATC AGAATTAGAG AAAACTATTG AACATTTGAC ATGTGTTAAT 
CATCAGCTTG AAAGCGAAAA ATATGAATTA AAGTCTAAAG TGTTAATAAT GAAAGAAACA 
ATAGAGTCGT TAGAGAACAA ATTAAAAGTC CAAGCTCAAA AATTTAGCCA TGTGGCTGGT 
GACTCATCTC ATCAGAAAAC AGAGGTGAAC TCACTTAGGA TAGTGAATGA GCAGCTACAG 
CGGTCAGTTG ATGACTATCA GCACCGACTT TCCATAAAAA GAGGTGAACT TGAATCAGCC 
CAAGCACAAA TTAAAATACT GGAGGAAAAG ATAGATGAAC TAAACCTTAA GATGACTTCA 
CAGGATGAGG AGGCTCATGT AATGAAAAAG ACCATTGGTG TTATTGATAA AGAAAAAGAC 
TTTCTCCAGG AGACTGTAGA TGAGAAGACA GAAAAGATTG CAAATTTGCA AGAAAACCTA 
GCTAATAAAG AAAAAGCTGT TGCTCAAATG AAGATAATGA TCTCAGAGTG TGAATCATCT 
GTGAACCAGC TGAAAGAAAC ATTGGTTAAT CGAGATCGTG AGATAAACAG CCTCCGGCGC 
CAGCTTGATG CAGCTCACAA AGAACTCGAT GAAGTAGGAA GATCTAGAGA AATCGCTTTT 
AAGGAAAACA GAAGACTGCA AGATGACCTG GCTACAATGG CAAGAGAAAA TCAAGAAATC 
TCATTGGAAT TGGAAGCAGC AGTGCAAGAA AAAGAAGAAA TGAAGAGCAG AGTTCATAAA 
TACATAACAG AGGTGTCACG ATGGGAGAGC TTAATGGCTG CCAAGGAAAA AGAAAATCAA 
GATTTGTTAG ATAGATTTCA GATGCTTCAT AACCGTGCTG AAGACTGGGA GGTCAAAGCC 
CATCAAGCTG AGGGAGAAAG CAGCTCAGTT CGACTGGAAC TTCTTTCTAT TGACACTGAG 
AGGAGACATC TTCGAGAAAG AGTGGAGCTA TTAGAAAAAG AAATTCAAGA GCACATAAAT 
GCCCATCATG CTTATGAATC TCAGATCTCA TCAATGGCAA AAGCCATGTC TCGATTAGAA 
GAAGAGCTGA GACATCAAGA AGATGAGAAA GCAACAGTAT TAAATGACTT GTCATCTCTT 
AGAGAACTTT GCATTAAACT TGATTCAGGC AAAGATATTA TGACCCAGCA ATTGAATTCG 
AAAAACCTTG AGTTTGAGAG GGTTGTGGTG GAATTAGAAA ATGTAAAGTC AGAGTCAGAC 
CTACTGAAAA AACAACTTTC AAATGAGAGA CATACAGTTA AAAACCTCGA ATCATTGTTG 
GCTACAAACA GAGATAAAGA ATTTCATTCT CACTTAACCT CCCACGAGAA GGATACAGAA 
ATCCAGCTAC TTAAGGAGAA GTTAACCCTT TCTGAAAGCA AATTAACTAG TCAAAGCCGG 
GAAAACACCA TGCTTCGAGC TAAAGTGGCA CAGTTACAAA CAGATTATGA TGCTCTGAAA 
AGGCAGATCT CAACTGAAAG ATACGAACGA GAACGAGCAA TCCAAGAGAT GCGTCGACAT 
GGTCTTGCTA CACCACCCCT TAGTTCCACT CTGAGGTCTC CTTCACATTC TCCTGAACAT 
AGAAATGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005265845.1
CDS	1359..3710
Translation	MQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	XM_005265788.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005265788.4

ORF Insert Sequence:

ATGCAGGATC TTGAAGAAAC AATGGCAAAA CTTCAGCTGG AATTGAACTT ATGCCAGAAA 
GAAAAGGAGA GACTGAGTGA TGAACTCCTT GTAAAATCAG ACCTAGAAAC TGTTGTTCAT 
CAGCTTGAAC AAGAAAAGCA AAGACTTAGC AAAAAAGTTG AAAGTTTTGC AGTTACAGAA 
CGACAACTTA CTCTGGAGGT TGAGAGGATG AGACTAGAAC ATGGAATAAA ACGTCGAGAC 
AGGTCACCTT CTCGTTTAGA TACATTTCTG AAAGGTATAG AAGAAGAACG AGATTATTAT 
AAGAAAGAGC TAGAGAGACT CCAACATATA ATACAGCGAA GATCTTGCTC TACAAGTTAT 
AGCGCACGTG AAAAAAGTTC AATATTTAGA ACACCAGAAA AGGGTGATTA CAATTCAGAA 
ATTCATCAGA TCACAAGAGA AAGAGATGAA CTTCAGCGTA TGCTAGAAAG ATTTGAAAAA 
TATATGGAAG ATATACAGTC CAATGTTAAA TTATTGACAG CAGAAAGAGA TAAACTAAGT 
GTCTTATATA ATGAAGCTCA GGAAGAATTA TCTGCCCTAA GAAAGGAATC CACCCAAACC 
ACAGCACCCC ATAATATTGT TAGTCTTATG GAAAAGGAAA AAGAACTTGC GTTATCTGAC 
TTAAGAAGAA TTATGGCAGA AAAGGAAGCT TTAAGAGAAA AATTAGAGCA TATTGAAGAA 
GTGAGTCTTT TTGGAAAATC AGAATTAGAG AAAACTATTG AACATTTGAC ATGTGTTAAT 
CATCAGCTTG AAAGCGAAAA ATATGAATTA AAGTCTAAAG TGTTAATAAT GAAAGAAACA 
ATAGAGTCGT TAGAGAACAA ATTAAAAGTC CAAGCTCAAA AATTTAGCCA TGTGGCTGGT 
GACTCATCTC ATCAGAAAAC AGAGGTGAAC TCACTTAGGA TAGTGAATGA GCAGCTACAG 
CGGTCAGTTG ATGACTATCA GCACCGACTT TCCATAAAAA GAGGTGAACT TGAATCAGCC 
CAAGCACAAA TTAAAATACT GGAGGAAAAG ATAGATGAAC TAAACCTTAA GATGACTTCA 
CAGGATGAGG AGGCTCATGT AATGAAAAAG ACCATTGGTG TTATTGATAA AGAAAAAGAC 
TTTCTCCAGG AGACTGTAGA TGAGAAGACA GAAAAGATTG CAAATTTGCA AGAAAACCTA 
GCTAATAAAG AAAAAGCTGT TGCTCAAATG AAGATAATGA TCTCAGAGTG TGAATCATCT 
GTGAACCAGC TGAAAGAAAC ATTGGTTAAT CGAGATCGTG AGATAAACAG CCTCCGGCGC 
CAGCTTGATG CAGCTCACAA AGAACTCGAT GAAGTAGGAA GATCTAGAGA AATCGCTTTT 
AAGGAAAACA GAAGACTGCA AGATGACCTG GCTACAATGG CAAGAGAAAA TCAAGAAATC 
TCATTGGAAT TGGAAGCAGC AGTGCAAGAA AAAGAAGAAA TGAAGAGCAG AGTTCATAAA 
TACATAACAG AGGTGTCACG ATGGGAGAGC TTAATGGCTG CCAAGGAAAA AGAAAATCAA 
GATTTGTTAG ATAGATTTCA GATGCTTCAT AACCGTGCTG AAGACTGGGA GGTCAAAGCC 
CATCAAGCTG AGGGAGAAAG CAGCTCAGTT CGACTGGAAC TTCTTTCTAT TGACACTGAG 
AGGAGACATC TTCGAGAAAG AGTGGAGCTA TTAGAAAAAG AAATTCAAGA GCACATAAAT 
GCCCATCATG CTTATGAATC TCAGATCTCA TCAATGGCAA AAGCCATGTC TCGATTAGAA 
GAAGAGCTGA GACATCAAGA AGATGAGAAA GCAACAGTAT TAAATGACTT GTCATCTCTT 
AGAGAACTTT GCATTAAACT TGATTCAGGC AAAGATATTA TGACCCAGCA ATTGAATTCG 
AAAAACCTTG AGTTTGAGAG GGTTGTGGTG GAATTAGAAA ATGTAAAGTC AGAGTCAGAC 
CTACTGAAAA AACAACTTTC AAATGAGAGA CATACAGTTA AAAACCTCGA ATCATTGTTG 
GCTACAAACA GAGATAAAGA ATTTCATTCT CACTTAACCT CCCACGAGAA GGATACAGAA 
ATCCAGCTAC TTAAGGAGAA GTTAACCCTT TCTGAAAGCA AATTAACTAG TCAAAGCCGG 
GAAAACACCA TGCTTCGAGC TAAAGTGGCA CAGTTACAAA CAGATTATGA TGCTCTGAAA 
AGGCAGATCT CAACTGAAAG ATACGAACGA GAACGAGCAA TCCAAGAGAT GCGTCGACAT 
GGTCTTGCTA CACCACCCCT TAGTTCCACT CTGAGGTCTC CTTCACATTC TCCTGAACAT 
AGAAATGTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP135 ( NM_025009.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_079285.2 Homo sapiens centrosomal protein 135 (CEP135), mRNA.

Price & Availability↑

CloneID	OHu20292
Clone ID Related Accession (Same CDS sequence)	NM_025009.4 , NM_025009.5
Accession Version	NM_025009.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA944890.1, BK005586.1, BC136535.1, CA438857.1, BU734129.1 and BF448516.1. This sequence is a reference standard in the RefSeqGene project. On Jun 16, 2012 this sequence version replaced NM_025009.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BK005586.1, SRR1660803.172259.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGAAAGAG ATTAAAAGAA AGCTCTCTGA AATGCAGGAT 
CTTGAAGAAA CAATGGCAAA ACTTCAGCTG GAATTGAACT TATGCCAGAA AGAAAAGGAG 
AGACTGAGTG ATGAACTCCT TGTAAAATCA GACCTAGAAA CTGTTGTTCA TCAGCTTGAA 
CAAGAAAAGC AAAGACTTAG CAAAAAAGTT GAAAGTTTTG CAGTTACAGA ACGACAACTT 
ACTCTGGAGG TTGAGAGGAT GAGACTAGAA CATGGAATAA AACGTCGAGA CAGGTCACCT 
TCTCGTTTAG ATACATTTCT GAAAGGTATA GAAGAAGAAC GAGATTATTA TAAGAAAGAG 
CTAGAGAGAC TCCAACATAT AATACAGCGA AGATCTTGCT CTACAAGTTA TAGCGCACGT 
GAAAAAAGTT CAATATTTAG AACACCAGAA AAGGGTGATT ACAATTCAGA AATTCATCAG 
ATCACAAGAG AAAGAGATGA ACTTCAGCGT ATGCTAGAAA GATTTGAAAA ATATATGGAA 
GATATACAGT CCAATGTTAA ATTATTGACA GCAGAAAGAG ATAAACTAAG TGTCTTATAT 
AATGAAGCTC AGGAAGAATT ATCTGCCCTA AGAAAGGAAT CCACCCAAAC CACAGCACCC 
CATAATATTG TTAGTCTTAT GGAAAAGGAA AAAGAACTTG CGTTATCTGA CTTAAGAAGA 
ATTATGGCAG AAAAGGAAGC TTTAAGAGAA AAATTAGAGC ATATTGAAGA AGTGAGTCTT 
TTTGGAAAAT CAGAATTAGA GAAAACTATT GAACATTTGA CATGTGTTAA TCATCAGCTT 
GAAAGCGAAA AATATGAATT AAAGTCTAAA GTGTTAATAA TGAAAGAAAC AATAGAGTCG 
TTAGAGAACA AATTAAAAGT CCAAGCTCAA AAATTTAGCC ATGTGGCTGG TGACTCATCT 
CATCAGAAAA CAGAGGTGAA CTCACTTAGG ATAGTGAATG AGCAGCTACA GCGGTCAGTT 
GATGACTATC AGCACCGACT TTCCATAAAA AGAGGTGAAC TTGAATCAGC CCAAGCACAA 
ATTAAAATAC TGGAGGAAAA GATAGATGAA CTAAACCTTA AGATGACTTC ACAGGATGAG 
GAGGCTCATG TAATGAAAAA GACCATTGGT GTTATTGATA AAGAAAAAGA CTTTCTCCAG 
GAGACTGTAG ATGAGAAGAC AGAAAAGATT GCAAATTTGC AAGAAAACCT AGCTAATAAA 
GAAAAAGCTG TTGCTCAAAT GAAGATAATG ATCTCAGAGT GTGAATCATC TGTGAACCAG 
CTGAAAGAAA CATTGGTTAA TCGAGATCGT GAGATAAACA GCCTCCGGCG CCAGCTTGAT 
GCAGCTCACA AAGAACTCGA TGAAGTAGGA AGATCTAGAG AAATCGCTTT TAAGGAAAAC 
AGAAGACTGC AAGATGACCT GGCTACAATG GCAAGAGAAA ATCAAGAAAT CTCATTGGAA 
TTGGAAGCAG CAGTGCAAGA AAAAGAAGAA ATGAAGAGCA GAGTTCATAA ATACATAACA 
GAGGTGTCAC GATGGGAGAG CTTAATGGCT GCCAAGGAAA AAGAAAATCA AGATTTGTTA 
GATAGATTTC AGATGCTTCA TAACCGTGCT GAAGACTGGG AGGTCAAAGC CCATCAAGCT 
GAGGGAGAAA GCAGCTCAGT TCGACTGGAA CTTCTTTCTA TTGACACTGA GAGGAGACAT 
CTTCGAGAAA GAGTGGAGCT ATTAGAAAAA GAAATTCAAG AGCACATAAA TGCCCATCAT 
GCTTATGAAT CTCAGATCTC ATCAATGGCA AAAGCCATGT CTCGATTAGA AGAAGAGCTG 
AGACATCAAG AAGATGAGAA AGCAACAGTA TTAAATGACT TGTCATCTCT TAGAGAACTT 
TGCATTAAAC TTGATTCAGG CAAAGATATT ATGACCCAGC AATTGAATTC GAAAAACCTT 
GAGTTTGAGA GGGTTGTGGT GGAATTAGAA AATGTAAAGT CAGAGTCAGA CCTACTGAAA 
AAACAACTTT CAAATGAGAG ACATACAGTT AAAAACCTCG AATCATTGTT GGCTACAAAC 
AGAGATAAAG AATTTCATTC TCACTTAACC TCCCACGAGA AGGATACAGA AATCCAGCTA 
CTTAAGGAGA AGTTAACCCT TTCTGAAAGC AAATTAACTA GTCAAAGCCG GGAAAACACC 
ATGCTTCGAG CTAAAGTGGC ACAGTTACAA ACAGATTATG ATGCTCTGAA AAGGCAGATC 
TCAACTGAAA GATACGAACG AGAACGAGCA ATCCAAGAGA TGCGTCGACA TGGTCTTGCT 
ACACCACCCC TTAGTTCCAC TCTGAGGTCT CCTTCACATT CTCCTGAACA TAGAAATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_079285.2
CDS	298..3720
Translation	MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKKEIKRKLSEMQDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	NM_025009.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_025009.4

ORF Insert Sequence:

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGAAAGAG ATTAAAAGAA AGCTCTCTGA AATGCAGGAT 
CTTGAAGAAA CAATGGCAAA ACTTCAGCTG GAATTGAACT TATGCCAGAA AGAAAAGGAG 
AGACTGAGTG ATGAACTCCT TGTAAAATCA GACCTAGAAA CTGTTGTTCA TCAGCTTGAA 
CAAGAAAAGC AAAGACTTAG CAAAAAAGTT GAAAGTTTTG CAGTTACAGA ACGACAACTT 
ACTCTGGAGG TTGAGAGGAT GAGACTAGAA CATGGAATAA AACGTCGAGA CAGGTCACCT 
TCTCGTTTAG ATACATTTCT GAAAGGTATA GAAGAAGAAC GAGATTATTA TAAGAAAGAG 
CTAGAGAGAC TCCAACATAT AATACAGCGA AGATCTTGCT CTACAAGTTA TAGCGCACGT 
GAAAAAAGTT CAATATTTAG AACACCAGAA AAGGGTGATT ACAATTCAGA AATTCATCAG 
ATCACAAGAG AAAGAGATGA ACTTCAGCGT ATGCTAGAAA GATTTGAAAA ATATATGGAA 
GATATACAGT CCAATGTTAA ATTATTGACA GCAGAAAGAG ATAAACTAAG TGTCTTATAT 
AATGAAGCTC AGGAAGAATT ATCTGCCCTA AGAAAGGAAT CCACCCAAAC CACAGCACCC 
CATAATATTG TTAGTCTTAT GGAAAAGGAA AAAGAACTTG CGTTATCTGA CTTAAGAAGA 
ATTATGGCAG AAAAGGAAGC TTTAAGAGAA AAATTAGAGC ATATTGAAGA AGTGAGTCTT 
TTTGGAAAAT CAGAATTAGA GAAAACTATT GAACATTTGA CATGTGTTAA TCATCAGCTT 
GAAAGCGAAA AATATGAATT AAAGTCTAAA GTGTTAATAA TGAAAGAAAC AATAGAGTCG 
TTAGAGAACA AATTAAAAGT CCAAGCTCAA AAATTTAGCC ATGTGGCTGG TGACTCATCT 
CATCAGAAAA CAGAGGTGAA CTCACTTAGG ATAGTGAATG AGCAGCTACA GCGGTCAGTT 
GATGACTATC AGCACCGACT TTCCATAAAA AGAGGTGAAC TTGAATCAGC CCAAGCACAA 
ATTAAAATAC TGGAGGAAAA GATAGATGAA CTAAACCTTA AGATGACTTC ACAGGATGAG 
GAGGCTCATG TAATGAAAAA GACCATTGGT GTTATTGATA AAGAAAAAGA CTTTCTCCAG 
GAGACTGTAG ATGAGAAGAC AGAAAAGATT GCAAATTTGC AAGAAAACCT AGCTAATAAA 
GAAAAAGCTG TTGCTCAAAT GAAGATAATG ATCTCAGAGT GTGAATCATC TGTGAACCAG 
CTGAAAGAAA CATTGGTTAA TCGAGATCGT GAGATAAACA GCCTCCGGCG CCAGCTTGAT 
GCAGCTCACA AAGAACTCGA TGAAGTAGGA AGATCTAGAG AAATCGCTTT TAAGGAAAAC 
AGAAGACTGC AAGATGACCT GGCTACAATG GCAAGAGAAA ATCAAGAAAT CTCATTGGAA 
TTGGAAGCAG CAGTGCAAGA AAAAGAAGAA ATGAAGAGCA GAGTTCATAA ATACATAACA 
GAGGTGTCAC GATGGGAGAG CTTAATGGCT GCCAAGGAAA AAGAAAATCA AGATTTGTTA 
GATAGATTTC AGATGCTTCA TAACCGTGCT GAAGACTGGG AGGTCAAAGC CCATCAAGCT 
GAGGGAGAAA GCAGCTCAGT TCGACTGGAA CTTCTTTCTA TTGACACTGA GAGGAGACAT 
CTTCGAGAAA GAGTGGAGCT ATTAGAAAAA GAAATTCAAG AGCACATAAA TGCCCATCAT 
GCTTATGAAT CTCAGATCTC ATCAATGGCA AAAGCCATGT CTCGATTAGA AGAAGAGCTG 
AGACATCAAG AAGATGAGAA AGCAACAGTA TTAAATGACT TGTCATCTCT TAGAGAACTT 
TGCATTAAAC TTGATTCAGG CAAAGATATT ATGACCCAGC AATTGAATTC GAAAAACCTT 
GAGTTTGAGA GGGTTGTGGT GGAATTAGAA AATGTAAAGT CAGAGTCAGA CCTACTGAAA 
AAACAACTTT CAAATGAGAG ACATACAGTT AAAAACCTCG AATCATTGTT GGCTACAAAC 
AGAGATAAAG AATTTCATTC TCACTTAACC TCCCACGAGA AGGATACAGA AATCCAGCTA 
CTTAAGGAGA AGTTAACCCT TTCTGAAAGC AAATTAACTA GTCAAAGCCG GGAAAACACC 
ATGCTTCGAG CTAAAGTGGC ACAGTTACAA ACAGATTATG ATGCTCTGAA AAGGCAGATC 
TCAACTGAAA GATACGAACG AGAACGAGCA ATCCAAGAGA TGCGTCGACA TGGTCTTGCT 
ACACCACCCC TTAGTTCCAC TCTGAGGTCT CCTTCACATT CTCCTGAACA TAGAAATGTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP135 ( XM_006714055.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714118.1 Homo sapiens centrosomal protein 135 (CEP135), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu38650
Clone ID Related Accession (Same CDS sequence)	XM_006714055.2 , XM_006714055.3
Accession Version	XM_006714055.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006714055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGGATCTT GAAGAAACAA TGGCAAAACT TCAGCTGGAA 
TTGAACTTAT GCCAGAAAGA AAAGGAGAGA CTGAGTGATG AACTCCTTGT AAAATCAGAC 
CTAGAAACTG TTGTTCATCA GCTTGAACAA GAAAAGCAAA GACTTAGCAA AAAAGTTGAA 
AGTTTTGCAG TTACAGAACG ACAACTTACT CTGGAGGTTG AGAGGATGAG ACTAGAACAT 
GGAATAAAAC GTCGAGACAG GTCACCTTCT CGTTTAGATA CATTTCTGAA AGGTATAGAA 
GAAGAACGAG ATTATTATAA GAAAGAGCTA GAGAGACTCC AACATATAAT ACAGCGAAGA 
TCTTGCTCTA CAAGTTATAG CGCACGTGAA AAAAGTTCAA TATTTAGAAC ACCAGAAAAG 
GGTGATTACA ATTCAGAAAT TCATCAGATC ACAAGAGAAA GAGATGAACT TCAGCGTATG 
CTAGAAAGAT TTGAAAAATA TATGGAAGAT ATACAGTCCA ATGTTAAATT ATTGACAGCA 
GAAAGAGATA AACTAAGTGT CTTATATAAT GAAGCTCAGG AAGAATTATC TGCCCTAAGA 
AAGGAATCCA CCCAAACCAC AGCACCCCAT AATATTGTTA GTCTTATGGA AAAGGAAAAA 
GAACTTGCGT TATCTGACTT AAGAAGAATT ATGGCAGAAA AGGAAGCTTT AAGAGAAAAA 
TTAGAGCATA TTGAAGAAGT GAGTCTTTTT GGAAAATCAG AATTAGAGAA AACTATTGAA 
CATTTGACAT GTGTTAATCA TCAGCTTGAA AGCGAAAAAT ATGAATTAAA GTCTAAAGTG 
TTAATAATGA AAGAAACAAT AGAGTCGTTA GAGAACAAAT TAAAAGTCCA AGCTCAAAAA 
TTTAGCCATG TGGCTGGTGA CTCATCTCAT CAGAAAACAG AGGTGAACTC ACTTAGGATA 
GTGAATGAGC AGCTACAGCG GTCAGTTGAT GACTATCAGC ACCGACTTTC CATAAAAAGA 
GGTGAACTTG AATCAGCCCA AGCACAAATT AAAATACTGG AGGAAAAGAT AGATGAACTA 
AACCTTAAGA TGACTTCACA GGATGAGGAG GCTCATGTAA TGAAAAAGAC CATTGGTGTT 
ATTGATAAAG AAAAAGACTT TCTCCAGGAG ACTGTAGATG AGAAGACAGA AAAGATTGCA 
AATTTGCAAG AAAACCTAGC TAATAAAGAA AAAGCTGTTG CTCAAATGAA GATAATGATC 
TCAGAGTGTG AATCATCTGT GAACCAGCTG AAAGAAACAT TGGTTAATCG AGATCGTGAG 
ATAAACAGCC TCCGGCGCCA GCTTGATGCA GCTCACAAAG AACTCGATGA AGTAGGAAGA 
TCTAGAGAAA TCGCTTTTAA GGAAAACAGA AGACTGCAAG ATGACCTGGC TACAATGGCA 
AGAGAAAATC AAGAAATCTC ATTGGAATTG GAAGCAGCAG TGCAAGAAAA AGAAGAAATG 
AAGAGCAGAG TTCATAAATA CATAACAGAG GTGTCACGAT GGGAGAGCTT AATGGCTGCC 
AAGGAAAAAG AAAATCAAGA TTTGTTAGAT AGATTTCAGA TGCTTCATAA CCGTGCTGAA 
GACTGGGAGG TCAAAGCCCA TCAAGCTGAG GGAGAAAGCA GCTCAGTTCG ACTGGAACTT 
CTTTCTATTG ACACTGAGAG GAGACATCTT CGAGAAAGAG TGGAGCTATT AGAAAAAGAA 
ATTCAAGAGC ACATAAATGC CCATCATGCT TATGAATCTC AGATCTCATC AATGGCAAAA 
GCCATGTCTC GATTAGAAGA AGAGCTGAGA CATCAAGAAG ATGAGAAAGC AACAGTATTA 
AATGACTTGT CATCTCTTAG AGAACTTTGC ATTAAACTTG ATTCAGGCAA AGATATTATG 
ACCCAGCAAT TGAATTCGAA AAACCTTGAG TTTGAGAGGG TTGTGGTGGA ATTAGAAAAT 
GTAAAGTCAG AGTCAGACCT ACTGAAAAAA CAACTTTCAA ATGAGAGACA TACAGTTAAA 
AACCTCGAAT CATTGTTGGC TACAAACAGA GATAAAGAAT TTCATTCTCA CTTAACCTCC 
CACGAGAAGG ATACAGAAAT CCAGCTACTT AAGGAGAAGT TAACCCTTTC TGAAAGCAAA 
TTAACTAGTC AAAGCCGGGA AAACACCATG CTTCGAGCTA AAGTGGCACA GTTACAAACA 
GATTATGATG CTCTGAAAAG GCAGATCTCA ACTGAAAGAT ACGAACGAGA ACGAGCAATC 
CAAGAGATGC GTCGACATGG TCTTGCTACA CCACCCCTTA GTTCCACTCT GAGGTCTCCT 
TCACATTCTC CTGAACATAG AAATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714118.1
CDS	298..3687
Translation	MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	XM_006714055.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714055.2

ORF Insert Sequence:

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGGATCTT GAAGAAACAA TGGCAAAACT TCAGCTGGAA 
TTGAACTTAT GCCAGAAAGA AAAGGAGAGA CTGAGTGATG AACTCCTTGT AAAATCAGAC 
CTAGAAACTG TTGTTCATCA GCTTGAACAA GAAAAGCAAA GACTTAGCAA AAAAGTTGAA 
AGTTTTGCAG TTACAGAACG ACAACTTACT CTGGAGGTTG AGAGGATGAG ACTAGAACAT 
GGAATAAAAC GTCGAGACAG GTCACCTTCT CGTTTAGATA CATTTCTGAA AGGTATAGAA 
GAAGAACGAG ATTATTATAA GAAAGAGCTA GAGAGACTCC AACATATAAT ACAGCGAAGA 
TCTTGCTCTA CAAGTTATAG CGCACGTGAA AAAAGTTCAA TATTTAGAAC ACCAGAAAAG 
GGTGATTACA ATTCAGAAAT TCATCAGATC ACAAGAGAAA GAGATGAACT TCAGCGTATG 
CTAGAAAGAT TTGAAAAATA TATGGAAGAT ATACAGTCCA ATGTTAAATT ATTGACAGCA 
GAAAGAGATA AACTAAGTGT CTTATATAAT GAAGCTCAGG AAGAATTATC TGCCCTAAGA 
AAGGAATCCA CCCAAACCAC AGCACCCCAT AATATTGTTA GTCTTATGGA AAAGGAAAAA 
GAACTTGCGT TATCTGACTT AAGAAGAATT ATGGCAGAAA AGGAAGCTTT AAGAGAAAAA 
TTAGAGCATA TTGAAGAAGT GAGTCTTTTT GGAAAATCAG AATTAGAGAA AACTATTGAA 
CATTTGACAT GTGTTAATCA TCAGCTTGAA AGCGAAAAAT ATGAATTAAA GTCTAAAGTG 
TTAATAATGA AAGAAACAAT AGAGTCGTTA GAGAACAAAT TAAAAGTCCA AGCTCAAAAA 
TTTAGCCATG TGGCTGGTGA CTCATCTCAT CAGAAAACAG AGGTGAACTC ACTTAGGATA 
GTGAATGAGC AGCTACAGCG GTCAGTTGAT GACTATCAGC ACCGACTTTC CATAAAAAGA 
GGTGAACTTG AATCAGCCCA AGCACAAATT AAAATACTGG AGGAAAAGAT AGATGAACTA 
AACCTTAAGA TGACTTCACA GGATGAGGAG GCTCATGTAA TGAAAAAGAC CATTGGTGTT 
ATTGATAAAG AAAAAGACTT TCTCCAGGAG ACTGTAGATG AGAAGACAGA AAAGATTGCA 
AATTTGCAAG AAAACCTAGC TAATAAAGAA AAAGCTGTTG CTCAAATGAA GATAATGATC 
TCAGAGTGTG AATCATCTGT GAACCAGCTG AAAGAAACAT TGGTTAATCG AGATCGTGAG 
ATAAACAGCC TCCGGCGCCA GCTTGATGCA GCTCACAAAG AACTCGATGA AGTAGGAAGA 
TCTAGAGAAA TCGCTTTTAA GGAAAACAGA AGACTGCAAG ATGACCTGGC TACAATGGCA 
AGAGAAAATC AAGAAATCTC ATTGGAATTG GAAGCAGCAG TGCAAGAAAA AGAAGAAATG 
AAGAGCAGAG TTCATAAATA CATAACAGAG GTGTCACGAT GGGAGAGCTT AATGGCTGCC 
AAGGAAAAAG AAAATCAAGA TTTGTTAGAT AGATTTCAGA TGCTTCATAA CCGTGCTGAA 
GACTGGGAGG TCAAAGCCCA TCAAGCTGAG GGAGAAAGCA GCTCAGTTCG ACTGGAACTT 
CTTTCTATTG ACACTGAGAG GAGACATCTT CGAGAAAGAG TGGAGCTATT AGAAAAAGAA 
ATTCAAGAGC ACATAAATGC CCATCATGCT TATGAATCTC AGATCTCATC AATGGCAAAA 
GCCATGTCTC GATTAGAAGA AGAGCTGAGA CATCAAGAAG ATGAGAAAGC AACAGTATTA 
AATGACTTGT CATCTCTTAG AGAACTTTGC ATTAAACTTG ATTCAGGCAA AGATATTATG 
ACCCAGCAAT TGAATTCGAA AAACCTTGAG TTTGAGAGGG TTGTGGTGGA ATTAGAAAAT 
GTAAAGTCAG AGTCAGACCT ACTGAAAAAA CAACTTTCAA ATGAGAGACA TACAGTTAAA 
AACCTCGAAT CATTGTTGGC TACAAACAGA GATAAAGAAT TTCATTCTCA CTTAACCTCC 
CACGAGAAGG ATACAGAAAT CCAGCTACTT AAGGAGAAGT TAACCCTTTC TGAAAGCAAA 
TTAACTAGTC AAAGCCGGGA AAACACCATG CTTCGAGCTA AAGTGGCACA GTTACAAACA 
GATTATGATG CTCTGAAAAG GCAGATCTCA ACTGAAAGAT ACGAACGAGA ACGAGCAATC 
CAAGAGATGC GTCGACATGG TCTTGCTACA CCACCCCTTA GTTCCACTCT GAGGTCTCCT 
TCACATTCTC CTGAACATAG AAATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP135 ( XM_006714055.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714118.1 Homo sapiens centrosomal protein 135 (CEP135), transcript variant X1, mRNA.

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CloneID	OHu38650
Clone ID Related Accession (Same CDS sequence)	XM_006714055.2 , XM_006714055.3
Accession Version	XM_006714055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 135 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006714055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGGATCTT GAAGAAACAA TGGCAAAACT TCAGCTGGAA 
TTGAACTTAT GCCAGAAAGA AAAGGAGAGA CTGAGTGATG AACTCCTTGT AAAATCAGAC 
CTAGAAACTG TTGTTCATCA GCTTGAACAA GAAAAGCAAA GACTTAGCAA AAAAGTTGAA 
AGTTTTGCAG TTACAGAACG ACAACTTACT CTGGAGGTTG AGAGGATGAG ACTAGAACAT 
GGAATAAAAC GTCGAGACAG GTCACCTTCT CGTTTAGATA CATTTCTGAA AGGTATAGAA 
GAAGAACGAG ATTATTATAA GAAAGAGCTA GAGAGACTCC AACATATAAT ACAGCGAAGA 
TCTTGCTCTA CAAGTTATAG CGCACGTGAA AAAAGTTCAA TATTTAGAAC ACCAGAAAAG 
GGTGATTACA ATTCAGAAAT TCATCAGATC ACAAGAGAAA GAGATGAACT TCAGCGTATG 
CTAGAAAGAT TTGAAAAATA TATGGAAGAT ATACAGTCCA ATGTTAAATT ATTGACAGCA 
GAAAGAGATA AACTAAGTGT CTTATATAAT GAAGCTCAGG AAGAATTATC TGCCCTAAGA 
AAGGAATCCA CCCAAACCAC AGCACCCCAT AATATTGTTA GTCTTATGGA AAAGGAAAAA 
GAACTTGCGT TATCTGACTT AAGAAGAATT ATGGCAGAAA AGGAAGCTTT AAGAGAAAAA 
TTAGAGCATA TTGAAGAAGT GAGTCTTTTT GGAAAATCAG AATTAGAGAA AACTATTGAA 
CATTTGACAT GTGTTAATCA TCAGCTTGAA AGCGAAAAAT ATGAATTAAA GTCTAAAGTG 
TTAATAATGA AAGAAACAAT AGAGTCGTTA GAGAACAAAT TAAAAGTCCA AGCTCAAAAA 
TTTAGCCATG TGGCTGGTGA CTCATCTCAT CAGAAAACAG AGGTGAACTC ACTTAGGATA 
GTGAATGAGC AGCTACAGCG GTCAGTTGAT GACTATCAGC ACCGACTTTC CATAAAAAGA 
GGTGAACTTG AATCAGCCCA AGCACAAATT AAAATACTGG AGGAAAAGAT AGATGAACTA 
AACCTTAAGA TGACTTCACA GGATGAGGAG GCTCATGTAA TGAAAAAGAC CATTGGTGTT 
ATTGATAAAG AAAAAGACTT TCTCCAGGAG ACTGTAGATG AGAAGACAGA AAAGATTGCA 
AATTTGCAAG AAAACCTAGC TAATAAAGAA AAAGCTGTTG CTCAAATGAA GATAATGATC 
TCAGAGTGTG AATCATCTGT GAACCAGCTG AAAGAAACAT TGGTTAATCG AGATCGTGAG 
ATAAACAGCC TCCGGCGCCA GCTTGATGCA GCTCACAAAG AACTCGATGA AGTAGGAAGA 
TCTAGAGAAA TCGCTTTTAA GGAAAACAGA AGACTGCAAG ATGACCTGGC TACAATGGCA 
AGAGAAAATC AAGAAATCTC ATTGGAATTG GAAGCAGCAG TGCAAGAAAA AGAAGAAATG 
AAGAGCAGAG TTCATAAATA CATAACAGAG GTGTCACGAT GGGAGAGCTT AATGGCTGCC 
AAGGAAAAAG AAAATCAAGA TTTGTTAGAT AGATTTCAGA TGCTTCATAA CCGTGCTGAA 
GACTGGGAGG TCAAAGCCCA TCAAGCTGAG GGAGAAAGCA GCTCAGTTCG ACTGGAACTT 
CTTTCTATTG ACACTGAGAG GAGACATCTT CGAGAAAGAG TGGAGCTATT AGAAAAAGAA 
ATTCAAGAGC ACATAAATGC CCATCATGCT TATGAATCTC AGATCTCATC AATGGCAAAA 
GCCATGTCTC GATTAGAAGA AGAGCTGAGA CATCAAGAAG ATGAGAAAGC AACAGTATTA 
AATGACTTGT CATCTCTTAG AGAACTTTGC ATTAAACTTG ATTCAGGCAA AGATATTATG 
ACCCAGCAAT TGAATTCGAA AAACCTTGAG TTTGAGAGGG TTGTGGTGGA ATTAGAAAAT 
GTAAAGTCAG AGTCAGACCT ACTGAAAAAA CAACTTTCAA ATGAGAGACA TACAGTTAAA 
AACCTCGAAT CATTGTTGGC TACAAACAGA GATAAAGAAT TTCATTCTCA CTTAACCTCC 
CACGAGAAGG ATACAGAAAT CCAGCTACTT AAGGAGAAGT TAACCCTTTC TGAAAGCAAA 
TTAACTAGTC AAAGCCGGGA AAACACCATG CTTCGAGCTA AAGTGGCACA GTTACAAACA 
GATTATGATG CTCTGAAAAG GCAGATCTCA ACTGAAAGAT ACGAACGAGA ACGAGCAATC 
CAAGAGATGC GTCGACATGG TCTTGCTACA CCACCCCTTA GTTCCACTCT GAGGTCTCCT 
TCACATTCTC CTGAACATAG AAATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714118.1
CDS	298..3687
Translation	MTTAVERKYINIRKRLDQLGYRQTLTVECLPLVEKLFSDLVHTTESLRQSKLSAVKAEKESANFDFVLEPYKLENARLSRENNELYLELMKLREHSDQHVKELKTSLKKCARETADLKFLNNQYAHKLKLLEKESKAKNERIQQLQEKNLHAVVQTPGGKKRSIAFRRQRMQIDEPVPPSEVSSYPVPQPDDPYIADLLQVADNRIQELQQEVHQLQEKLAMMESGVRDYSKQIELREREIERLSVALDGGRSPDVLSLESRNKTNEKLIAHLNIQVDFLQQANKDLEKRIRELMETKETVTSEVVNLSNKNEKLCQELTEIDQLAQQLERHKEEVLETADKELGEAKDLEETMAKLQLELNLCQKEKERLSDELLVKSDLETVVHQLEQEKQRLSKKVESFAVTERQLTLEVERMRLEHGIKRRDRSPSRLDTFLKGIEEERDYYKKELERLQHIIQRRSCSTSYSAREKSSIFRTPEKGDYNSEIHQITRERDELQRMLERFEKYMEDIQSNVKLLTAERDKLSVLYNEAQEELSALRKESTQTTAPHNIVSLMEKEKELALSDLRRIMAEKEALREKLEHIEEVSLFGKSELEKTIEHLTCVNHQLESEKYELKSKVLIMKETIESLENKLKVQAQKFSHVAGDSSHQKTEVNSLRIVNEQLQRSVDDYQHRLSIKRGELESAQAQIKILEEKIDELNLKMTSQDEEAHVMKKTIGVIDKEKDFLQETVDEKTEKIANLQENLANKEKAVAQMKIMISECESSVNQLKETLVNRDREINSLRRQLDAAHKELDEVGRSREIAFKENRRLQDDLATMARENQEISLELEAAVQEKEEMKSRVHKYITEVSRWESLMAAKEKENQDLLDRFQMLHNRAEDWEVKAHQAEGESSSVRLELLSIDTERRHLRERVELLEKEIQEHINAHHAYESQISSMAKAMSRLEEELRHQEDEKATVLNDLSSLRELCIKLDSGKDIMTQQLNSKNLEFERVVVELENVKSESDLLKKQLSNERHTVKNLESLLATNRDKEFHSHLTSHEKDTEIQLLKEKLTLSESKLTSQSRENTMLRAKVAQLQTDYDALKRQISTERYERERAIQEMRRHGLATPPLSSTLRSPSHSPEHRNV

Target ORF information:

RefSeq Version	XM_006714055.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 135 (CEP135), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714055.3

ORF Insert Sequence:

ATGACTACAG CTGTAGAGAG AAAGTATATT AATATTAGGA AAAGGCTGGA TCAGCTGGGA 
TACCGCCAGA CTCTGACAGT GGAGTGTTTA CCTTTGGTAG AAAAACTTTT CAGCGACTTA 
GTTCATACAA CTGAGAGCCT TCGGCAATCA AAATTATCTG CTGTGAAAGC TGAAAAAGAA 
AGTGCCAATT TTGATTTTGT TTTGGAACCC TATAAACTTG AAAATGCAAG ATTGAGTAGA 
GAAAATAATG AATTATACCT AGAGTTAATG AAACTGAGAG AACATTCAGA CCAACACGTT 
AAAGAGTTGA AAACTTCATT GAAGAAATGT GCACGTGAAA CAGCTGATCT GAAATTTCTG 
AATAACCAAT ATGCTCATAA ACTCAAACTG TTGGAGAAAG AGAGCAAAGC TAAGAATGAA 
AGAATTCAAC AACTTCAAGA AAAGAATTTG CATGCTGTAG TACAAACTCC AGGTGGCAAG 
AAAAGAAGTA TTGCTTTCAG GCGCCAGCGT ATGCAAATTG ATGAACCGGT TCCTCCCTCT 
GAAGTCAGTT CATATCCAGT TCCTCAACCA GATGACCCTT ACATTGCAGA CCTCCTTCAA 
GTGGCTGATA ACAGGATTCA AGAACTTCAA CAGGAAGTCC ACCAGCTACA AGAAAAGTTA 
GCAATGATGG AAAGTGGGGT GAGAGACTAT AGCAAGCAGA TTGAGCTAAG AGAACGAGAG 
ATAGAACGAC TGTCAGTTGC TTTGGATGGT GGTCGGTCCC CTGATGTCCT TTCTCTGGAG 
TCTAGAAATA AAACCAATGA AAAGCTTATT GCTCATTTAA ATATTCAGGT TGACTTTCTT 
CAGCAAGCTA ATAAAGACCT GGAGAAGCGT ATACGAGAGC TTATGGAAAC CAAGGAAACA 
GTGACATCTG AAGTCGTTAA TTTAAGTAAC AAAAATGAAA AACTCTGCCA AGAATTAACT 
GAAATAGATC AGTTAGCACA GCAGTTGGAA AGACATAAAG AAGAAGTGCT TGAGACTGCT 
GATAAAGAGC TTGGGGAAGC AAAGGATCTT GAAGAAACAA TGGCAAAACT TCAGCTGGAA 
TTGAACTTAT GCCAGAAAGA AAAGGAGAGA CTGAGTGATG AACTCCTTGT AAAATCAGAC 
CTAGAAACTG TTGTTCATCA GCTTGAACAA GAAAAGCAAA GACTTAGCAA AAAAGTTGAA 
AGTTTTGCAG TTACAGAACG ACAACTTACT CTGGAGGTTG AGAGGATGAG ACTAGAACAT 
GGAATAAAAC GTCGAGACAG GTCACCTTCT CGTTTAGATA CATTTCTGAA AGGTATAGAA 
GAAGAACGAG ATTATTATAA GAAAGAGCTA GAGAGACTCC AACATATAAT ACAGCGAAGA 
TCTTGCTCTA CAAGTTATAG CGCACGTGAA AAAAGTTCAA TATTTAGAAC ACCAGAAAAG 
GGTGATTACA ATTCAGAAAT TCATCAGATC ACAAGAGAAA GAGATGAACT TCAGCGTATG 
CTAGAAAGAT TTGAAAAATA TATGGAAGAT ATACAGTCCA ATGTTAAATT ATTGACAGCA 
GAAAGAGATA AACTAAGTGT CTTATATAAT GAAGCTCAGG AAGAATTATC TGCCCTAAGA 
AAGGAATCCA CCCAAACCAC AGCACCCCAT AATATTGTTA GTCTTATGGA AAAGGAAAAA 
GAACTTGCGT TATCTGACTT AAGAAGAATT ATGGCAGAAA AGGAAGCTTT AAGAGAAAAA 
TTAGAGCATA TTGAAGAAGT GAGTCTTTTT GGAAAATCAG AATTAGAGAA AACTATTGAA 
CATTTGACAT GTGTTAATCA TCAGCTTGAA AGCGAAAAAT ATGAATTAAA GTCTAAAGTG 
TTAATAATGA AAGAAACAAT AGAGTCGTTA GAGAACAAAT TAAAAGTCCA AGCTCAAAAA 
TTTAGCCATG TGGCTGGTGA CTCATCTCAT CAGAAAACAG AGGTGAACTC ACTTAGGATA 
GTGAATGAGC AGCTACAGCG GTCAGTTGAT GACTATCAGC ACCGACTTTC CATAAAAAGA 
GGTGAACTTG AATCAGCCCA AGCACAAATT AAAATACTGG AGGAAAAGAT AGATGAACTA 
AACCTTAAGA TGACTTCACA GGATGAGGAG GCTCATGTAA TGAAAAAGAC CATTGGTGTT 
ATTGATAAAG AAAAAGACTT TCTCCAGGAG ACTGTAGATG AGAAGACAGA AAAGATTGCA 
AATTTGCAAG AAAACCTAGC TAATAAAGAA AAAGCTGTTG CTCAAATGAA GATAATGATC 
TCAGAGTGTG AATCATCTGT GAACCAGCTG AAAGAAACAT TGGTTAATCG AGATCGTGAG 
ATAAACAGCC TCCGGCGCCA GCTTGATGCA GCTCACAAAG AACTCGATGA AGTAGGAAGA 
TCTAGAGAAA TCGCTTTTAA GGAAAACAGA AGACTGCAAG ATGACCTGGC TACAATGGCA 
AGAGAAAATC AAGAAATCTC ATTGGAATTG GAAGCAGCAG TGCAAGAAAA AGAAGAAATG 
AAGAGCAGAG TTCATAAATA CATAACAGAG GTGTCACGAT GGGAGAGCTT AATGGCTGCC 
AAGGAAAAAG AAAATCAAGA TTTGTTAGAT AGATTTCAGA TGCTTCATAA CCGTGCTGAA 
GACTGGGAGG TCAAAGCCCA TCAAGCTGAG GGAGAAAGCA GCTCAGTTCG ACTGGAACTT 
CTTTCTATTG ACACTGAGAG GAGACATCTT CGAGAAAGAG TGGAGCTATT AGAAAAAGAA 
ATTCAAGAGC ACATAAATGC CCATCATGCT TATGAATCTC AGATCTCATC AATGGCAAAA 
GCCATGTCTC GATTAGAAGA AGAGCTGAGA CATCAAGAAG ATGAGAAAGC AACAGTATTA 
AATGACTTGT CATCTCTTAG AGAACTTTGC ATTAAACTTG ATTCAGGCAA AGATATTATG 
ACCCAGCAAT TGAATTCGAA AAACCTTGAG TTTGAGAGGG TTGTGGTGGA ATTAGAAAAT 
GTAAAGTCAG AGTCAGACCT ACTGAAAAAA CAACTTTCAA ATGAGAGACA TACAGTTAAA 
AACCTCGAAT CATTGTTGGC TACAAACAGA GATAAAGAAT TTCATTCTCA CTTAACCTCC 
CACGAGAAGG ATACAGAAAT CCAGCTACTT AAGGAGAAGT TAACCCTTTC TGAAAGCAAA 
TTAACTAGTC AAAGCCGGGA AAACACCATG CTTCGAGCTA AAGTGGCACA GTTACAAACA 
GATTATGATG CTCTGAAAAG GCAGATCTCA ACTGAAAGAT ACGAACGAGA ACGAGCAATC 
CAAGAGATGC GTCGACATGG TCTTGCTACA CCACCCCTTA GTTCCACTCT GAGGTCTCCT 
TCACATTCTC CTGAACATAG AAATGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Human Centriolar Protein CEP135 Contains a Two-Stranded Coiled-Coil Domain Critical for Microtubule Binding.
Structure (London, England : 1993)24(8)1358-1371(2016 Aug)
Kraatz S,Guichard P,Obbineni JM,Olieric N,Hatzopoulos GN,Hilbert M,Sen I,Missimer J,G?nczy P,Steinmetz MO

CEP295 interacts with microtubules and is required for centriole elongation.
Journal of cell science129(13)2501-13(2016 07)
Chang CW,Hsu WB,Tsai JJ,Tang CJ,Tang TK

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

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CEP135 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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