MDC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDC1
Entrez Gene ID	9656
Full Name	mediator of DNA damage checkpoint 1
Synonyms	NFBD1
General protein information	Preferred Names mediator of DNA damage checkpoint 1 Names mediator of DNA damage checkpoint protein 1 homologue to Drosophila photoreceptor protein calphotin nuclear factor with BRCT domains 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.33
Summary	The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008].
Disorder MIM:	607593

mRNA and Protein(s)

mRNA	Protein	Name
XM_005249493.3	XP_005249550.1	mediator of DNA damage checkpoint protein 1 isoform X2
XM_011547335.1	XP_011545637.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011547336.1	XP_011545638.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011547950.1	XP_011546252.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011547334.1	XP_011545636.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011548121.1	XP_011546423.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011515002.1	XP_011513304.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_005249494.4	XP_005249551.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_011515003.2	XP_011513305.1	mediator of DNA damage checkpoint protein 1 isoform X3
NM_014641.3	NP_055456.2	mediator of DNA damage checkpoint protein 1
XM_017011521.2	XP_016867010.1	mediator of DNA damage checkpoint protein 1 isoform X7
XM_005249498.4	XP_005249555.1	mediator of DNA damage checkpoint protein 1 isoform X8
XM_017011522.2	XP_016867011.1	mediator of DNA damage checkpoint protein 1 isoform X9
XM_005249497.3	XP_005249554.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_005249494.3	XP_005249551.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011515001.1	XP_011513303.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011548120.1	XP_011546422.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_017011520.1	XP_016867009.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_011515004.3	XP_011513306.1	mediator of DNA damage checkpoint protein 1 isoform X5
XM_011547948.1	XP_011546250.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_005249492.2	XP_005249549.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_011515001.3	XP_011513303.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_005249494.5	XP_005249551.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_017011520.2	XP_016867009.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_005249497.4	XP_005249554.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_017011521.1	XP_016867010.1	mediator of DNA damage checkpoint protein 1 isoform X7
XM_005249498.3	XP_005249555.1	mediator of DNA damage checkpoint protein 1 isoform X8
XM_011548122.1	XP_011546424.1	mediator of DNA damage checkpoint protein 1 isoform X6
NM_014641.2	NP_055456.2	mediator of DNA damage checkpoint protein 1
XM_017011522.1	XP_016867011.1	mediator of DNA damage checkpoint protein 1 isoform X9
XM_005249493.4	XP_005249550.1	mediator of DNA damage checkpoint protein 1 isoform X2
XM_011515003.1	XP_011513305.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_005272911.3	XP_005272968.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_005275323.3	XP_005275380.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011547949.1	XP_011546251.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_005275196.3	XP_005275253.1	mediator of DNA damage checkpoint protein 1 isoform X6
XM_011515001.2	XP_011513303.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_017011519.1	XP_016867008.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_011515004.2	XP_011513306.1	mediator of DNA damage checkpoint protein 1 isoform X5
XM_017011519.2	XP_016867008.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_011515003.3	XP_011513305.1	mediator of DNA damage checkpoint protein 1 isoform X3

Pathways from BioSystems

Homologies

	MDC1	NP_001108419.1
Pan troglodytes (chimpanzee)	MDC1	NP_001035841.1
Canis lupus familiaris (dog)	MDC1	XP_005627164.1
Homo sapiens (human)	MDC1	NP_055456.2
Bos taurus (cattle)	MDC1	XP_002697401.2
Mus musculus (house mouse)	Mdc1	NP_001010833.2
Rattus norvegicus (Norway rat)	Mdc1	NP_001159747.1

Gene Ontology
Bibliography

Related articles in PubMed

SOX9 activity is induced by oncogenic Kras to affect MDC1 and MCMs expression in pancreatic cancer.
Zhou H, Qin Y, Ji S, Ling J, Fu J, Zhuang Z, Fan X, Song L, Yu X, Chiao PJ
Oncogene(2017 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ASF1a promotes non-homologous end joining repair by facilitating phosphorylation of MDC1 by ATM at double-strand breaks.
Title: ASF1a Promotes Non-homologous End Joining Repair by Facilitating Phosphorylation of MDC1 by ATM at Double-Strand Breaks.

It showed a link between the status of MDC1 protein and TP53 gene, which specific mutations caused radiation-induced MDC1 down-regulation.
Title: Mutations in the TP53 gene affected recruitment of 53BP1 protein to DNA lesions, but level of 53BP1 was stable after ?-irradiation that depleted MDC1 protein in specific TP53 mutants.

the opposing activities of RNF4 and ataxin-3 consolidate robust MDC1-dependent signaling and repair ofDNA double-strand break.
Title: Ataxin-3 consolidates the MDC1-dependent DNA double-strand break response by counteracting the SUMO-targeted ubiquitin ligase RNF4.

NFBD1 protein is overexpressed in NPC tissues and that silencing NFBD1 can inhibit cell growth, induce apoptosis, increase the production of intracellular ROS. NFBD1 knockdown also inhibits the tumorigenicity of NPC cells in vivo.
Title: Depletion of NFBD1/MDC1 Induces Apoptosis in Nasopharyngeal Carcinoma Cells Through the p53-ROS-Mitochondrial Pathway.

knockdown of MCM2 or MCM6 could significantly inhibit foci forming of MDC1 in TE-1 nuclei in response to bleomycin-induced DNA damage (p < 0.001). This study indicates the direct interaction between MDC1 and MCMs in TE-1 nuclei.
Title: Knockdown of Minichromosome Maintenance Proteins Inhibits Foci Forming of Mediator of DNA-Damage Checkpoint 1 in Response to DNA Damage in Human Esophageal Squamous Cell Carcinoma TE-1 Cells.

The following MDC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06599	NM_014641.3 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011547334.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011547336.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_005249494.5 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011547335.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515001.3 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011547950.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515002.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_005275196.3 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515003.3 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011548120.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_005275323.3 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_005272911.3 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011547949.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011547948.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_017011519.2 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011548121.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011548122.1 Latest version!	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu40542	XM_005249492.2 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu40543	XM_005249493.4 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu40544	XM_005249497.4 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu40545	XM_005249498.4 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu55010	XM_017011520.2 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu55011	XM_017011521.2 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu63099	XM_011515004.3 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu96934	XM_017011522.2 Latest version!	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu06599	XM_005249494.3	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515001.1	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_005249494.4	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu55010	XM_017011520.1	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu40544	XM_005249497.3	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu55011	XM_017011521.1	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu40545	XM_005249498.3	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu06599	NM_014641.2	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu96934	XM_017011522.1	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu40543	XM_005249493.3	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515003.1	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515001.2	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu63099	XM_011515004.2	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu06599	XM_017011519.1	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
OHu06599	XM_011515003.2	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDC1 ( NM_014641.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055456.2 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	NM_014641.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF177823.1, D79992.1, AL662797.7 and BU621433.1. On Nov 22, 2018 this sequence version replaced NM_014641.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D79992.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000376406.8/ ENSP00000365588.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055456.2
CDS	41..6310
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	NM_014641.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014641.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( NM_014641.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055456.2 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	NM_014641.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF177823.1, D79992.1, AL662797.7 and BU621433.1. On Mar 15, 2007 this sequence version replaced NM_014641.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D79992.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055456.2
CDS	441..6710
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	NM_014641.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014641.2

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011547335.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011545637.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011547335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011545637.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011547335.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011547335.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011547336.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011545638.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011547336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011545638.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011547336.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011547336.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011547950.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011546252.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011547950.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011546252.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011547950.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011547950.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011547334.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011545636.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011547334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011545636.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011547334.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011547334.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011548121.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011546423.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011548121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011546423.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011548121.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011548121.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513304.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011513304.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515002.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515002.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249494.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249551.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_005249494.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249494.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249551.1
CDS	744..7013
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249494.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249494.4

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515003.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513305.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515003.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513305.1
CDS	27..6296
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515003.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515003.2

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249494.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249551.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_005249494.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005249551.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249494.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249494.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515001.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513303.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515001.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011513303.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515001.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515001.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011548120.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011546422.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011548120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011546422.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011548120.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011548120.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011547948.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011546250.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011547948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011546250.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011547948.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011547948.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515001.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513303.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515001.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515001.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513303.1
CDS	172..6441
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515001.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515001.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249494.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249551.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_005249494.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005249494.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249551.1
CDS	744..7013
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249494.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249494.5

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011548122.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011546424.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011548122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011546424.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011548122.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011548122.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515003.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513305.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515003.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011513305.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515003.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515003.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005272911.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272968.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_005272911.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005272968.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005272911.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272911.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005275323.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005275380.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_005275323.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005275380.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005275323.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005275323.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011547949.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011546251.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011547949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011546251.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011547949.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011547949.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005275196.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005275253.1 Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_005275196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_005275253.1

Translation

MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005275196.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mediator of DNA-damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005275196.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515001.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513303.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515001.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513303.1
CDS	172..6441
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515001.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515001.2

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011519.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867008.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_017011519.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867008.1
CDS	124..6393
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011519.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011519.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011519.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867008.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_017011519.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867008.1
CDS	124..6393
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011519.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011519.2

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515003.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513305.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OHu06599
Clone ID Related Accession (Same CDS sequence)	XM_011547335.1 , XM_011547336.1 , XM_011547950.1 , XM_011547334.1 , XM_011548121.1 , XM_011515002.1 , XM_005249494.4 , XM_011515003.2 , NM_014641.3 , XM_005249494.3 , XM_011515001.1 , XM_011548120.1 , XM_011547948.1 , XM_011515001.3 , XM_005249494.5 , XM_011548122.1 , NM_014641.2 , XM_011515003.1 , XM_005272911.3 , XM_005275323.3 , XM_011547949.1 , XM_005275196.3 , XM_011515001.2 , XM_017011519.1 , XM_017011519.2 , XM_011515003.3
Accession Version	XM_011515003.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515003.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513305.1
CDS	27..6296
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515003.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515003.3

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249492.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249549.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

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CloneID	OHu40542
Clone ID Related Accession (Same CDS sequence)	XM_005249492.2
Accession Version	XM_005249492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005249492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGTACCC TAGATGAACC ATGGGAGGTC CTGGCTACAC AGCCATTCTG TCTGAGAGAG 
TCTGAGGACT CTGAGACCCA GCCTTTTGAC ACGCACCTTG AGGCCTATGG ACCTTGCCTG 
TCTCCACCTA GGGCAATACC AGGAGACCAA CATCCAGAGA GCCCAGTTCA CACAGAGCCA 
ATGGGGATTC AAGGCAGAGG GAGGCAGACT GTGGATAAAG TCATGGGTAT ACCAAAAGAA 
ACAGCAGAGA GGGTGGGCCC TGAGAGAGGG CCATTGGAGA GAGAAACTGA GAAACTGCTA 
CCAGAAAGAC AGACAGATGT GACAGGAGAG GAAGAATTAA CCAAGGGGAA ACAGGACAGA 
GAACAAAAAC AGTTGTTAGC TAGAGACACC CAGAGACAAG AATCTGACAA AAATGGGGAA 
AGTGCAAGTC CTGAAAGAGA TAGGGAGAGT TTGAAGGTAG AAATTGAGAC ATCTGAGGAA 
ATACAAGAGA AACAAGTACA GAAGCAGACC CTTCCAAGCA AAGCATTTGA GAGAGAAGTA 
GAGAGACCAG TAGCAAACAG AGAGTGCGAT CCAGCCGAGT TAGAAGAGAA GGTGCCCAAA 
GTGATCCTGG AGAGAGATAC ACAGAGAGGG GAGCCAGAGG GAGGGAGCCA GGACCAGAAA 
GGGCAGGCCT CCAGCCCAAC ACCAGAGCCT GGGGTGGGGG CGGGGGACCT TCCGGGACCT 
ACCTCAGCCC CCGTACCTTC TGGGAGCCAG TCAGGTGGAA GGGGATCCCC AGTGAGCCCC 
AGGAGGCATC AGAAAGGCCT CCTGAATTGC AAGATGCCAC CTGCTGAGAA GGCTTCCAGG 
ATCAGAGCTG CTGAGAAGGT TTCCAGGGGC GATCAGGAAT CTCCAGATGC TTGTCTGCCT 
CCTACAGTAC CTGAAGCCCC AGCCCCACCC CAAAAGCCCC TTAACTCTCA GAGCCAGAAA 
CATCTTGCAC CTCCGCCCCT TCTTTCTCCC CTTTTACCTT CTATCAAGCC AACCGTTCGT 
AAGACCAGGC AAGATGGGAG TCAGGAAGCT CCAGAGGCTC CCTTGTCCTC AGAGCTGGAG 
CCTTTCCACC CAAAGCCTAA AATTAGAACT CGGAAGTCCT CCAGAATGAC ACCCTTTCCA 
GCTACCTCTG CTGCCCCTGA GCCCCACCCT TCCACCTCCA CAGCCCAGCC AGTCACTCCC 
AAGCCCACAT CTCAGGCCAC TAGGAGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAA 
CCAGTTGTCC CCACAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCCTGTCACC 
TCTGAGCCCA CATCTCAGGT TACTAGGGGA AGAAAAAGTA GATCCTCTGT CAAGACCCCT 
GAAACAGTTG TGCCCACAGC CCTTGAGCTC CAGCCTTCCA CCTCCACCGA CCGACCTGTC 
ACCTCTGAAC CCACCTCTCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTCCCCAC AGCCCCTGAG CTCCAGCCTT CCACCTCCAC AGACCAGCCT 
GTCACTTCTG AGCCCACATA TCAGGCTACT AGGGGAAGAA AAAATAGATC CTCTGTCAAG 
ACCCCTGAAC CAGTTGTGCC CACAGCCCCT GAGCTCCGGC CTTCCACCTC CACAGACCGA 
CCTGTCACCC CCAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTC 
AAGACCCCTG AAACAGTTGT CCCCACAGCC CCTGAGCTCC AGATTTCCAC CTCCACAGAC 
CAACCTGTCA CCCCTAAGCC CACATCTCGG ACCACTAGGA GCAGGACAAA TATGTCCTCT 
GTGAAGAACC CTGAATCAAC TGTCCCTATA GCCCCTGAGC TCCCACCTTC CACCTCCACA 
GAGCAGCCTG TCACCCCTGA GCCCACATCT CGGGCTACTA GGGGAAGAAA AAATAGATCC 
TCTGGCAAGA CCCCTGAAAC ACTTGTCCCC ACAGCCCCTA AGCTCGAGCC TTCCACTTCC 
ACAGACCAAC CTGTCACTCC TGAGCCCACA TCTCAGGCCA CCAGGGGCAG GACAAATAGG 
TCCTCTGTGA AGACCCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTCCA GCCTTCCACC 
TCCACAGACC AGCCTGTTAC CCCTGAGCCT ACGTCTCAGG CTACTAGGGG AAGAACAGAT 
AGATCCTCTG TCAAGACTCC TGAAACAGTT GTCCCCACAG CCCCTGAGCT ACAGGCTTCC 
GCCTCCACAG ACCAGCCTGT CACCTCTGAG CCCACATCTC GGACCACTAG GGGAAGAAAA 
AATCGGTCCT CTGTCAAGAC CCCTGAAACA GTTGTGCCCG CAGCCCCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAACC TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGGGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAA TCAATTGTCC CTATAGCCCC TGAGCTTCAG 
CCTTCCACCT CCAGAAACCA GCTTGTCACC CCTGAGCCCA CATCTCGGGC CACTAGGTGC 
AGGACAAATA GGTCCTCTGT CAAGACCCCT GAGCCAGTTG TCCCCACAGC CCCTGAGCCC 
CATCCTACCA CCTCCACAGA CCAGCCTGTC ACCCCCAAGC TCACATCTAG GGCCACTAGG 
AGAAAGACAA ATAGGTCCTC TGTCAAGACT CCCAAACCAG TTGAACCAGC AGCCTCTGAT 
CTTGAGCCTT TTACCCCCAC AGACCAGTCC GTCACCCCTG AGGCCATAGC TCAGGGTGGT 
CAGAGCAAAA CACTGAGGTC TTCCACAGTA AGAGCTATGC CGGTTCCTAC CACCCCTGAA 
TTCCAATCTC CTGTCACCAC AGACCAGCCT ATTTCCCCTG AGCCTATTAC TCAACCCAGT 
TGCATCAAGA GGCAGAGAGC CGCTGGGAAC CCTGGCTCCC TCGCAGCTCC CATTGACCAT 
AAGCCTTGCT CTGCACCCTT GGAACCTAAA TCCCAGGCCT CAAGGAACCA AAGATGGGGA 
GCAGTGAGAG CAGCTGAATC CCTTACAGCC ATTCCTGAGC CTGCCTCTCC CCAGCTTCTT 
GAGACACCAA TTCATGCCTC CCAGATCCAA AAGGTGGAAC CAGCAGGTAG ATCTAGGTTC 
ACCCCGGAGC TCCAGCCTAA GGCCTCTCAA AGCCGCAAGA GGTCTTTAGC TACCATGGAT 
TCACCACCAC ATCAAAAACA GCCCCAAAGA GGGGAAGTCT CCCAGAAGAC AGTGATTATC 
AAGGAAGAGG AAGAAGATAC TGCAGAGAAG CCAGGGAAGG AAGAGGATGT CGTGACTCCA 
AAACCAGGCA AGAGAAAGAG AGACCAGGCA GAGGAGGAGC CCAACAGAAT ACCAAGCCGC 
AGCCTCCGAC GGACCAAACT TAACCAAGAA TCAACAGCCC CCAAAGTGCT CTTCACAGGA 
GTGGTGGATG CTCGGGGAGA GCGGGCTGTG CTGGCACTGG GGGGAAGTCT GGCTGGTTCA 
GCGGCAGAGG CTTCCCACCT GGTCACTGAT CGCATCCGCC GGACAGTCAA GTTCCTGTGT 
GCCCTGGGGC GGGGAATCCC CATTCTGTCC CTGGACTGGC TGCATCAGTC CCGCAAGGCT 
GGTTTCTTCT TACCCCCGGA TGAATATGTG GTGACCGACC CTGAGCAAGA GAAGAACTTT 
GGCTTTAGCC TTCAAGACGC ACTGAGCAGG GCTCGGGAGC GAAGGCTGCT AGAGGGCTAT 
GAGATCTATG TGACCCCTGG AGTCCAGCCA CCACCACCTC AGATGGGAGA GATTATTAGC 
TGCTGTGGAG GCACATACCT ACCCAGCATG CCTCGGTCCT ATAAGCCTCA GAGAGTTGTG 
ATCACATGCC CTCAGGACTT CCCTCATTGC TCCATTCCAC TACGGGTTGG GCTGCCCCTC 
CTCTCGCCTG AGTTCCTGCT GACTGGAGTG CTGAAGCAGG AAGCCAAGCC AGAGGCCTTT 
GTCCTCTCCC CTTTGGAGAT GTCATCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249549.1
CDS	282..6614
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249492.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249492.2

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGTACCC TAGATGAACC ATGGGAGGTC CTGGCTACAC AGCCATTCTG TCTGAGAGAG 
TCTGAGGACT CTGAGACCCA GCCTTTTGAC ACGCACCTTG AGGCCTATGG ACCTTGCCTG 
TCTCCACCTA GGGCAATACC AGGAGACCAA CATCCAGAGA GCCCAGTTCA CACAGAGCCA 
ATGGGGATTC AAGGCAGAGG GAGGCAGACT GTGGATAAAG TCATGGGTAT ACCAAAAGAA 
ACAGCAGAGA GGGTGGGCCC TGAGAGAGGG CCATTGGAGA GAGAAACTGA GAAACTGCTA 
CCAGAAAGAC AGACAGATGT GACAGGAGAG GAAGAATTAA CCAAGGGGAA ACAGGACAGA 
GAACAAAAAC AGTTGTTAGC TAGAGACACC CAGAGACAAG AATCTGACAA AAATGGGGAA 
AGTGCAAGTC CTGAAAGAGA TAGGGAGAGT TTGAAGGTAG AAATTGAGAC ATCTGAGGAA 
ATACAAGAGA AACAAGTACA GAAGCAGACC CTTCCAAGCA AAGCATTTGA GAGAGAAGTA 
GAGAGACCAG TAGCAAACAG AGAGTGCGAT CCAGCCGAGT TAGAAGAGAA GGTGCCCAAA 
GTGATCCTGG AGAGAGATAC ACAGAGAGGG GAGCCAGAGG GAGGGAGCCA GGACCAGAAA 
GGGCAGGCCT CCAGCCCAAC ACCAGAGCCT GGGGTGGGGG CGGGGGACCT TCCGGGACCT 
ACCTCAGCCC CCGTACCTTC TGGGAGCCAG TCAGGTGGAA GGGGATCCCC AGTGAGCCCC 
AGGAGGCATC AGAAAGGCCT CCTGAATTGC AAGATGCCAC CTGCTGAGAA GGCTTCCAGG 
ATCAGAGCTG CTGAGAAGGT TTCCAGGGGC GATCAGGAAT CTCCAGATGC TTGTCTGCCT 
CCTACAGTAC CTGAAGCCCC AGCCCCACCC CAAAAGCCCC TTAACTCTCA GAGCCAGAAA 
CATCTTGCAC CTCCGCCCCT TCTTTCTCCC CTTTTACCTT CTATCAAGCC AACCGTTCGT 
AAGACCAGGC AAGATGGGAG TCAGGAAGCT CCAGAGGCTC CCTTGTCCTC AGAGCTGGAG 
CCTTTCCACC CAAAGCCTAA AATTAGAACT CGGAAGTCCT CCAGAATGAC ACCCTTTCCA 
GCTACCTCTG CTGCCCCTGA GCCCCACCCT TCCACCTCCA CAGCCCAGCC AGTCACTCCC 
AAGCCCACAT CTCAGGCCAC TAGGAGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAA 
CCAGTTGTCC CCACAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCCTGTCACC 
TCTGAGCCCA CATCTCAGGT TACTAGGGGA AGAAAAAGTA GATCCTCTGT CAAGACCCCT 
GAAACAGTTG TGCCCACAGC CCTTGAGCTC CAGCCTTCCA CCTCCACCGA CCGACCTGTC 
ACCTCTGAAC CCACCTCTCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTCCCCAC AGCCCCTGAG CTCCAGCCTT CCACCTCCAC AGACCAGCCT 
GTCACTTCTG AGCCCACATA TCAGGCTACT AGGGGAAGAA AAAATAGATC CTCTGTCAAG 
ACCCCTGAAC CAGTTGTGCC CACAGCCCCT GAGCTCCGGC CTTCCACCTC CACAGACCGA 
CCTGTCACCC CCAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTC 
AAGACCCCTG AAACAGTTGT CCCCACAGCC CCTGAGCTCC AGATTTCCAC CTCCACAGAC 
CAACCTGTCA CCCCTAAGCC CACATCTCGG ACCACTAGGA GCAGGACAAA TATGTCCTCT 
GTGAAGAACC CTGAATCAAC TGTCCCTATA GCCCCTGAGC TCCCACCTTC CACCTCCACA 
GAGCAGCCTG TCACCCCTGA GCCCACATCT CGGGCTACTA GGGGAAGAAA AAATAGATCC 
TCTGGCAAGA CCCCTGAAAC ACTTGTCCCC ACAGCCCCTA AGCTCGAGCC TTCCACTTCC 
ACAGACCAAC CTGTCACTCC TGAGCCCACA TCTCAGGCCA CCAGGGGCAG GACAAATAGG 
TCCTCTGTGA AGACCCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTCCA GCCTTCCACC 
TCCACAGACC AGCCTGTTAC CCCTGAGCCT ACGTCTCAGG CTACTAGGGG AAGAACAGAT 
AGATCCTCTG TCAAGACTCC TGAAACAGTT GTCCCCACAG CCCCTGAGCT ACAGGCTTCC 
GCCTCCACAG ACCAGCCTGT CACCTCTGAG CCCACATCTC GGACCACTAG GGGAAGAAAA 
AATCGGTCCT CTGTCAAGAC CCCTGAAACA GTTGTGCCCG CAGCCCCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAACC TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGGGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAA TCAATTGTCC CTATAGCCCC TGAGCTTCAG 
CCTTCCACCT CCAGAAACCA GCTTGTCACC CCTGAGCCCA CATCTCGGGC CACTAGGTGC 
AGGACAAATA GGTCCTCTGT CAAGACCCCT GAGCCAGTTG TCCCCACAGC CCCTGAGCCC 
CATCCTACCA CCTCCACAGA CCAGCCTGTC ACCCCCAAGC TCACATCTAG GGCCACTAGG 
AGAAAGACAA ATAGGTCCTC TGTCAAGACT CCCAAACCAG TTGAACCAGC AGCCTCTGAT 
CTTGAGCCTT TTACCCCCAC AGACCAGTCC GTCACCCCTG AGGCCATAGC TCAGGGTGGT 
CAGAGCAAAA CACTGAGGTC TTCCACAGTA AGAGCTATGC CGGTTCCTAC CACCCCTGAA 
TTCCAATCTC CTGTCACCAC AGACCAGCCT ATTTCCCCTG AGCCTATTAC TCAACCCAGT 
TGCATCAAGA GGCAGAGAGC CGCTGGGAAC CCTGGCTCCC TCGCAGCTCC CATTGACCAT 
AAGCCTTGCT CTGCACCCTT GGAACCTAAA TCCCAGGCCT CAAGGAACCA AAGATGGGGA 
GCAGTGAGAG CAGCTGAATC CCTTACAGCC ATTCCTGAGC CTGCCTCTCC CCAGCTTCTT 
GAGACACCAA TTCATGCCTC CCAGATCCAA AAGGTGGAAC CAGCAGGTAG ATCTAGGTTC 
ACCCCGGAGC TCCAGCCTAA GGCCTCTCAA AGCCGCAAGA GGTCTTTAGC TACCATGGAT 
TCACCACCAC ATCAAAAACA GCCCCAAAGA GGGGAAGTCT CCCAGAAGAC AGTGATTATC 
AAGGAAGAGG AAGAAGATAC TGCAGAGAAG CCAGGGAAGG AAGAGGATGT CGTGACTCCA 
AAACCAGGCA AGAGAAAGAG AGACCAGGCA GAGGAGGAGC CCAACAGAAT ACCAAGCCGC 
AGCCTCCGAC GGACCAAACT TAACCAAGAA TCAACAGCCC CCAAAGTGCT CTTCACAGGA 
GTGGTGGATG CTCGGGGAGA GCGGGCTGTG CTGGCACTGG GGGGAAGTCT GGCTGGTTCA 
GCGGCAGAGG CTTCCCACCT GGTCACTGAT CGCATCCGCC GGACAGTCAA GTTCCTGTGT 
GCCCTGGGGC GGGGAATCCC CATTCTGTCC CTGGACTGGC TGCATCAGTC CCGCAAGGCT 
GGTTTCTTCT TACCCCCGGA TGAATATGTG GTGACCGACC CTGAGCAAGA GAAGAACTTT 
GGCTTTAGCC TTCAAGACGC ACTGAGCAGG GCTCGGGAGC GAAGGCTGCT AGAGGGCTAT 
GAGATCTATG TGACCCCTGG AGTCCAGCCA CCACCACCTC AGATGGGAGA GATTATTAGC 
TGCTGTGGAG GCACATACCT ACCCAGCATG CCTCGGTCCT ATAAGCCTCA GAGAGTTGTG 
ATCACATGCC CTCAGGACTT CCCTCATTGC TCCATTCCAC TACGGGTTGG GCTGCCCCTC 
CTCTCGCCTG AGTTCCTGCT GACTGGAGTG CTGAAGCAGG AAGCCAAGCC AGAGGCCTTT 
GTCCTCTCCC CTTTGGAGAT GTCATCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249493.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249550.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

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CloneID	OHu40543
Clone ID Related Accession (Same CDS sequence)	XM_005249493.3 , XM_005249493.4
Accession Version	XM_005249493.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249493.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249550.1
CDS	282..6611
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249493.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249493.3

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249493.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249550.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

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CloneID	OHu40543
Clone ID Related Accession (Same CDS sequence)	XM_005249493.3 , XM_005249493.4
Accession Version	XM_005249493.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005249493.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249550.1
CDS	282..6611
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249493.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249493.4

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGTATACC AAAAGAAACA 
GCAGAGAGGG TGGGCCCTGA GAGAGGGCCA TTGGAGAGAG AAACTGAGAA ACTGCTACCA 
GAAAGACAGA CAGATGTGAC AGGAGAGGAA GAATTAACCA AGGGGAAACA GGACAGAGAA 
CAAAAACAGT TGTTAGCTAG AGACACCCAG AGACAAGAAT CTGACAAAAA TGGGGAAAGT 
GCAAGTCCTG AAAGAGATAG GGAGAGTTTG AAGGTAGAAA TTGAGACATC TGAGGAAATA 
CAAGAGAAAC AAGTACAGAA GCAGACCCTT CCAAGCAAAG CATTTGAGAG AGAAGTAGAG 
AGACCAGTAG CAAACAGAGA GTGCGATCCA GCCGAGTTAG AAGAGAAGGT GCCCAAAGTG 
ATCCTGGAGA GAGATACACA GAGAGGGGAG CCAGAGGGAG GGAGCCAGGA CCAGAAAGGG 
CAGGCCTCCA GCCCAACACC AGAGCCTGGG GTGGGGGCGG GGGACCTTCC GGGACCTACC 
TCAGCCCCCG TACCTTCTGG GAGCCAGTCA GGTGGAAGGG GATCCCCAGT GAGCCCCAGG 
AGGCATCAGA AAGGCCTCCT GAATTGCAAG ATGCCACCTG CTGAGAAGGC TTCCAGGATC 
AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT CAGGAATCTC CAGATGCTTG TCTGCCTCCT 
ACAGTACCTG AAGCCCCAGC CCCACCCCAA AAGCCCCTTA ACTCTCAGAG CCAGAAACAT 
CTTGCACCTC CGCCCCTTCT TTCTCCCCTT TTACCTTCTA TCAAGCCAAC CGTTCGTAAG 
ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT 
TTCCACCCAA AGCCTAAAAT TAGAACTCGG AAGTCCTCCA GAATGACACC CTTTCCAGCT 
ACCTCTGCTG CCCCTGAGCC CCACCCTTCC ACCTCCACAG CCCAGCCAGT CACTCCCAAG 
CCCACATCTC AGGCCACTAG GAGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAACCA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCAGGTTAC TAGGGGAAGA AAAAGTAGAT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCACAGCCCT TGAGCTCCAG CCTTCCACCT CCACCGACCG ACCTGTCACC 
TCTGAACCCA CCTCTCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TCCCCACAGC CCCTGAGCTC CAGCCTTCCA CCTCCACAGA CCAGCCTGTC 
ACTTCTGAGC CCACATATCA GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGTCAAGACC 
CCTGAACCAG TTGTGCCCAC AGCCCCTGAG CTCCGGCCTT CCACCTCCAC AGACCGACCT 
GTCACCCCCA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTCAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGA TTTCCACCTC CACAGACCAA 
CCTGTCACCC CTAAGCCCAC ATCTCGGACC ACTAGGAGCA GGACAAATAT GTCCTCTGTG 
AAGAACCCTG AATCAACTGT CCCTATAGCC CCTGAGCTCC CACCTTCCAC CTCCACAGAG 
CAGCCTGTCA CCCCTGAGCC CACATCTCGG GCTACTAGGG GAAGAAAAAA TAGATCCTCT 
GGCAAGACCC CTGAAACACT TGTCCCCACA GCCCCTAAGC TCGAGCCTTC CACTTCCACA 
GACCAACCTG TCACTCCTGA GCCCACATCT CAGGCCACCA GGGGCAGGAC AAATAGGTCC 
TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTCCAGCC TTCCACCTCC 
ACAGACCAGC CTGTTACCCC TGAGCCTACG TCTCAGGCTA CTAGGGGAAG AACAGATAGA 
TCCTCTGTCA AGACTCCTGA AACAGTTGTC CCCACAGCCC CTGAGCTACA GGCTTCCGCC 
TCCACAGACC AGCCTGTCAC CTCTGAGCCC ACATCTCGGA CCACTAGGGG AAGAAAAAAT 
CGGTCCTCTG TCAAGACCCC TGAAACAGTT GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC 
ACCTCCACAG ACCAACCTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GGGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAATCA ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT 
TCCACCTCCA GAAACCAGCT TGTCACCCCT GAGCCCACAT CTCGGGCCAC TAGGTGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAG CCAGTTGTCC CCACAGCCCC TGAGCCCCAT 
CCTACCACCT CCACAGACCA GCCTGTCACC CCCAAGCTCA CATCTAGGGC CACTAGGAGA 
AAGACAAATA GGTCCTCTGT CAAGACTCCC AAACCAGTTG AACCAGCAGC CTCTGATCTT 
GAGCCTTTTA CCCCCACAGA CCAGTCCGTC ACCCCTGAGG CCATAGCTCA GGGTGGTCAG 
AGCAAAACAC TGAGGTCTTC CACAGTAAGA GCTATGCCGG TTCCTACCAC CCCTGAATTC 
CAATCTCCTG TCACCACAGA CCAGCCTATT TCCCCTGAGC CTATTACTCA ACCCAGTTGC 
ATCAAGAGGC AGAGAGCCGC TGGGAACCCT GGCTCCCTCG CAGCTCCCAT TGACCATAAG 
CCTTGCTCTG CACCCTTGGA ACCTAAATCC CAGGCCTCAA GGAACCAAAG ATGGGGAGCA 
GTGAGAGCAG CTGAATCCCT TACAGCCATT CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG 
ACACCAATTC ATGCCTCCCA GATCCAAAAG GTGGAACCAG CAGGTAGATC TAGGTTCACC 
CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC CGCAAGAGGT CTTTAGCTAC CATGGATTCA 
CCACCACATC AAAAACAGCC CCAAAGAGGG GAAGTCTCCC AGAAGACAGT GATTATCAAG 
GAAGAGGAAG AAGATACTGC AGAGAAGCCA GGGAAGGAAG AGGATGTCGT GACTCCAAAA 
CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG GAGGAGCCCA ACAGAATACC AAGCCGCAGC 
CTCCGACGGA CCAAACTTAA CCAAGAATCA ACAGCCCCCA AAGTGCTCTT CACAGGAGTG 
GTGGATGCTC GGGGAGAGCG GGCTGTGCTG GCACTGGGGG GAAGTCTGGC TGGTTCAGCG 
GCAGAGGCTT CCCACCTGGT CACTGATCGC ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC 
CTGGGGCGGG GAATCCCCAT TCTGTCCCTG GACTGGCTGC ATCAGTCCCG CAAGGCTGGT 
TTCTTCTTAC CCCCGGATGA ATATGTGGTG ACCGACCCTG AGCAAGAGAA GAACTTTGGC 
TTTAGCCTTC AAGACGCACT GAGCAGGGCT CGGGAGCGAA GGCTGCTAGA GGGCTATGAG 
ATCTATGTGA CCCCTGGAGT CCAGCCACCA CCACCTCAGA TGGGAGAGAT TATTAGCTGC 
TGTGGAGGCA CATACCTACC CAGCATGCCT CGGTCCTATA AGCCTCAGAG AGTTGTGATC 
ACATGCCCTC AGGACTTCCC TCATTGCTCC ATTCCACTAC GGGTTGGGCT GCCCCTCCTC 
TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC 
CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249497.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249554.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OHu40544
Clone ID Related Accession (Same CDS sequence)	XM_005249497.3 , XM_005249497.4
Accession Version	XM_005249497.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249497.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGTACCC TAGATGAACC ATGGGAGGTC CTGGCTACAC AGCCATTCTG TCTGAGAGAG 
TCTGAGGACT CTGAGACCCA GCCTTTTGAC ACGCACCTTG AGGCCTATGG ACCTTGCCTG 
TCTCCACCTA GGGCAATACC AGGAGACCAA CATCCAGAGA GCCCAGTTCA CACAGAGCCA 
ATGGGGATTC AAGGCAGAGG GAGGCAGACT GTGGATAAAG TCATGGGCCT CCTGAATTGC 
AAGATGCCAC CTGCTGAGAA GGCTTCCAGG ATCAGAGCTG CTGAGAAGGT TTCCAGGGGC 
GATCAGGAAT CTCCAGATGC TTGTCTGCCT CCTACAGTAC CTGAAGCCCC AGCCCCACCC 
CAAAAGCCCC TTAACTCTCA GAGCCAGAAA CATCTTGCAC CTCCGCCCCT TCTTTCTCCC 
CTTTTACCTT CTATCAAGCC AACCGTTCGT AAGACCAGGC AAGATGGGAG TCAGGAAGCT 
CCAGAGGCTC CCTTGTCCTC AGAGCTGGAG CCTTTCCACC CAAAGCCTAA AATTAGAACT 
CGGAAGTCCT CCAGAATGAC ACCCTTTCCA GCTACCTCTG CTGCCCCTGA GCCCCACCCT 
TCCACCTCCA CAGCCCAGCC AGTCACTCCC AAGCCCACAT CTCAGGCCAC TAGGAGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAA CCAGTTGTCC CCACAGCCCC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCCTGTCACC TCTGAGCCCA CATCTCAGGT TACTAGGGGA 
AGAAAAAGTA GATCCTCTGT CAAGACCCCT GAAACAGTTG TGCCCACAGC CCTTGAGCTC 
CAGCCTTCCA CCTCCACCGA CCGACCTGTC ACCTCTGAAC CCACCTCTCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTCCCCAC AGCCCCTGAG 
CTCCAGCCTT CCACCTCCAC AGACCAGCCT GTCACTTCTG AGCCCACATA TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTGCC CACAGCCCCT 
GAGCTCCGGC CTTCCACCTC CACAGACCGA CCTGTCACCC CCAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTC AAGACCCCTG AAACAGTTGT CCCCACAGCC 
CCTGAGCTCC AGATTTCCAC CTCCACAGAC CAACCTGTCA CCCCTAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTGAAGAACC CTGAATCAAC TGTCCCTATA 
GCCCCTGAGC TCCCACCTTC CACCTCCACA GAGCAGCCTG TCACCCCTGA GCCCACATCT 
CGGGCTACTA GGGGAAGAAA AAATAGATCC TCTGGCAAGA CCCCTGAAAC ACTTGTCCCC 
ACAGCCCCTA AGCTCGAGCC TTCCACTTCC ACAGACCAAC CTGTCACTCC TGAGCCCACA 
TCTCAGGCCA CCAGGGGCAG GACAAATAGG TCCTCTGTGA AGACCCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTTAC CCCTGAGCCT 
ACGTCTCAGG CTACTAGGGG AAGAACAGAT AGATCCTCTG TCAAGACTCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT ACAGGCTTCC GCCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC GGACCACTAG GGGAAGAAAA AATCGGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCG CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAACC TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGGGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAA 
TCAATTGTCC CTATAGCCCC TGAGCTTCAG CCTTCCACCT CCAGAAACCA GCTTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGTGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAGCCAGTTG TCCCCACAGC CCCTGAGCCC CATCCTACCA CCTCCACAGA CCAGCCTGTC 
ACCCCCAAGC TCACATCTAG GGCCACTAGG AGAAAGACAA ATAGGTCCTC TGTCAAGACT 
CCCAAACCAG TTGAACCAGC AGCCTCTGAT CTTGAGCCTT TTACCCCCAC AGACCAGTCC 
GTCACCCCTG AGGCCATAGC TCAGGGTGGT CAGAGCAAAA CACTGAGGTC TTCCACAGTA 
AGAGCTATGC CGGTTCCTAC CACCCCTGAA TTCCAATCTC CTGTCACCAC AGACCAGCCT 
ATTTCCCCTG AGCCTATTAC TCAACCCAGT TGCATCAAGA GGCAGAGAGC CGCTGGGAAC 
CCTGGCTCCC TCGCAGCTCC CATTGACCAT AAGCCTTGCT CTGCACCCTT GGAACCTAAA 
TCCCAGGCCT CAAGGAACCA AAGATGGGGA GCAGTGAGAG CAGCTGAATC CCTTACAGCC 
ATTCCTGAGC CTGCCTCTCC CCAGCTTCTT GAGACACCAA TTCATGCCTC CCAGATCCAA 
AAGGTGGAAC CAGCAGGTAG ATCTAGGTTC ACCCCGGAGC TCCAGCCTAA GGCCTCTCAA 
AGCCGCAAGA GGTCTTTAGC TACCATGGAT TCACCACCAC ATCAAAAACA GCCCCAAAGA 
GGGGAAGTCT CCCAGAAGAC AGTGATTATC AAGGAAGAGG AAGAAGATAC TGCAGAGAAG 
CCAGGGAAGG AAGAGGATGT CGTGACTCCA AAACCAGGCA AGAGAAAGAG AGACCAGGCA 
GAGGAGGAGC CCAACAGAAT ACCAAGCCGC AGCCTCCGAC GGACCAAACT TAACCAAGAA 
TCAACAGCCC CCAAAGTGCT CTTCACAGGA GTGGTGGATG CTCGGGGAGA GCGGGCTGTG 
CTGGCACTGG GGGGAAGTCT GGCTGGTTCA GCGGCAGAGG CTTCCCACCT GGTCACTGAT 
CGCATCCGCC GGACAGTCAA GTTCCTGTGT GCCCTGGGGC GGGGAATCCC CATTCTGTCC 
CTGGACTGGC TGCATCAGTC CCGCAAGGCT GGTTTCTTCT TACCCCCGGA TGAATATGTG 
GTGACCGACC CTGAGCAAGA GAAGAACTTT GGCTTTAGCC TTCAAGACGC ACTGAGCAGG 
GCTCGGGAGC GAAGGCTGCT AGAGGGCTAT GAGATCTATG TGACCCCTGG AGTCCAGCCA 
CCACCACCTC AGATGGGAGA GATTATTAGC TGCTGTGGAG GCACATACCT ACCCAGCATG 
CCTCGGTCCT ATAAGCCTCA GAGAGTTGTG ATCACATGCC CTCAGGACTT CCCTCATTGC 
TCCATTCCAC TACGGGTTGG GCTGCCCCTC CTCTCGCCTG AGTTCCTGCT GACTGGAGTG 
CTGAAGCAGG AAGCCAAGCC AGAGGCCTTT GTCCTCTCCC CTTTGGAGAT GTCATCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249554.1
CDS	282..6044
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249497.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249497.3

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGTACCC TAGATGAACC ATGGGAGGTC CTGGCTACAC AGCCATTCTG TCTGAGAGAG 
TCTGAGGACT CTGAGACCCA GCCTTTTGAC ACGCACCTTG AGGCCTATGG ACCTTGCCTG 
TCTCCACCTA GGGCAATACC AGGAGACCAA CATCCAGAGA GCCCAGTTCA CACAGAGCCA 
ATGGGGATTC AAGGCAGAGG GAGGCAGACT GTGGATAAAG TCATGGGCCT CCTGAATTGC 
AAGATGCCAC CTGCTGAGAA GGCTTCCAGG ATCAGAGCTG CTGAGAAGGT TTCCAGGGGC 
GATCAGGAAT CTCCAGATGC TTGTCTGCCT CCTACAGTAC CTGAAGCCCC AGCCCCACCC 
CAAAAGCCCC TTAACTCTCA GAGCCAGAAA CATCTTGCAC CTCCGCCCCT TCTTTCTCCC 
CTTTTACCTT CTATCAAGCC AACCGTTCGT AAGACCAGGC AAGATGGGAG TCAGGAAGCT 
CCAGAGGCTC CCTTGTCCTC AGAGCTGGAG CCTTTCCACC CAAAGCCTAA AATTAGAACT 
CGGAAGTCCT CCAGAATGAC ACCCTTTCCA GCTACCTCTG CTGCCCCTGA GCCCCACCCT 
TCCACCTCCA CAGCCCAGCC AGTCACTCCC AAGCCCACAT CTCAGGCCAC TAGGAGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAA CCAGTTGTCC CCACAGCCCC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCCTGTCACC TCTGAGCCCA CATCTCAGGT TACTAGGGGA 
AGAAAAAGTA GATCCTCTGT CAAGACCCCT GAAACAGTTG TGCCCACAGC CCTTGAGCTC 
CAGCCTTCCA CCTCCACCGA CCGACCTGTC ACCTCTGAAC CCACCTCTCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTCCCCAC AGCCCCTGAG 
CTCCAGCCTT CCACCTCCAC AGACCAGCCT GTCACTTCTG AGCCCACATA TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTGCC CACAGCCCCT 
GAGCTCCGGC CTTCCACCTC CACAGACCGA CCTGTCACCC CCAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTC AAGACCCCTG AAACAGTTGT CCCCACAGCC 
CCTGAGCTCC AGATTTCCAC CTCCACAGAC CAACCTGTCA CCCCTAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTGAAGAACC CTGAATCAAC TGTCCCTATA 
GCCCCTGAGC TCCCACCTTC CACCTCCACA GAGCAGCCTG TCACCCCTGA GCCCACATCT 
CGGGCTACTA GGGGAAGAAA AAATAGATCC TCTGGCAAGA CCCCTGAAAC ACTTGTCCCC 
ACAGCCCCTA AGCTCGAGCC TTCCACTTCC ACAGACCAAC CTGTCACTCC TGAGCCCACA 
TCTCAGGCCA CCAGGGGCAG GACAAATAGG TCCTCTGTGA AGACCCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTTAC CCCTGAGCCT 
ACGTCTCAGG CTACTAGGGG AAGAACAGAT AGATCCTCTG TCAAGACTCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT ACAGGCTTCC GCCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC GGACCACTAG GGGAAGAAAA AATCGGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCG CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAACC TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGGGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAA 
TCAATTGTCC CTATAGCCCC TGAGCTTCAG CCTTCCACCT CCAGAAACCA GCTTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGTGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAGCCAGTTG TCCCCACAGC CCCTGAGCCC CATCCTACCA CCTCCACAGA CCAGCCTGTC 
ACCCCCAAGC TCACATCTAG GGCCACTAGG AGAAAGACAA ATAGGTCCTC TGTCAAGACT 
CCCAAACCAG TTGAACCAGC AGCCTCTGAT CTTGAGCCTT TTACCCCCAC AGACCAGTCC 
GTCACCCCTG AGGCCATAGC TCAGGGTGGT CAGAGCAAAA CACTGAGGTC TTCCACAGTA 
AGAGCTATGC CGGTTCCTAC CACCCCTGAA TTCCAATCTC CTGTCACCAC AGACCAGCCT 
ATTTCCCCTG AGCCTATTAC TCAACCCAGT TGCATCAAGA GGCAGAGAGC CGCTGGGAAC 
CCTGGCTCCC TCGCAGCTCC CATTGACCAT AAGCCTTGCT CTGCACCCTT GGAACCTAAA 
TCCCAGGCCT CAAGGAACCA AAGATGGGGA GCAGTGAGAG CAGCTGAATC CCTTACAGCC 
ATTCCTGAGC CTGCCTCTCC CCAGCTTCTT GAGACACCAA TTCATGCCTC CCAGATCCAA 
AAGGTGGAAC CAGCAGGTAG ATCTAGGTTC ACCCCGGAGC TCCAGCCTAA GGCCTCTCAA 
AGCCGCAAGA GGTCTTTAGC TACCATGGAT TCACCACCAC ATCAAAAACA GCCCCAAAGA 
GGGGAAGTCT CCCAGAAGAC AGTGATTATC AAGGAAGAGG AAGAAGATAC TGCAGAGAAG 
CCAGGGAAGG AAGAGGATGT CGTGACTCCA AAACCAGGCA AGAGAAAGAG AGACCAGGCA 
GAGGAGGAGC CCAACAGAAT ACCAAGCCGC AGCCTCCGAC GGACCAAACT TAACCAAGAA 
TCAACAGCCC CCAAAGTGCT CTTCACAGGA GTGGTGGATG CTCGGGGAGA GCGGGCTGTG 
CTGGCACTGG GGGGAAGTCT GGCTGGTTCA GCGGCAGAGG CTTCCCACCT GGTCACTGAT 
CGCATCCGCC GGACAGTCAA GTTCCTGTGT GCCCTGGGGC GGGGAATCCC CATTCTGTCC 
CTGGACTGGC TGCATCAGTC CCGCAAGGCT GGTTTCTTCT TACCCCCGGA TGAATATGTG 
GTGACCGACC CTGAGCAAGA GAAGAACTTT GGCTTTAGCC TTCAAGACGC ACTGAGCAGG 
GCTCGGGAGC GAAGGCTGCT AGAGGGCTAT GAGATCTATG TGACCCCTGG AGTCCAGCCA 
CCACCACCTC AGATGGGAGA GATTATTAGC TGCTGTGGAG GCACATACCT ACCCAGCATG 
CCTCGGTCCT ATAAGCCTCA GAGAGTTGTG ATCACATGCC CTCAGGACTT CCCTCATTGC 
TCCATTCCAC TACGGGTTGG GCTGCCCCTC CTCTCGCCTG AGTTCCTGCT GACTGGAGTG 
CTGAAGCAGG AAGCCAAGCC AGAGGCCTTT GTCCTCTCCC CTTTGGAGAT GTCATCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249497.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249554.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OHu40544
Clone ID Related Accession (Same CDS sequence)	XM_005249497.3 , XM_005249497.4
Accession Version	XM_005249497.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005249497.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGTACCC TAGATGAACC ATGGGAGGTC CTGGCTACAC AGCCATTCTG TCTGAGAGAG 
TCTGAGGACT CTGAGACCCA GCCTTTTGAC ACGCACCTTG AGGCCTATGG ACCTTGCCTG 
TCTCCACCTA GGGCAATACC AGGAGACCAA CATCCAGAGA GCCCAGTTCA CACAGAGCCA 
ATGGGGATTC AAGGCAGAGG GAGGCAGACT GTGGATAAAG TCATGGGCCT CCTGAATTGC 
AAGATGCCAC CTGCTGAGAA GGCTTCCAGG ATCAGAGCTG CTGAGAAGGT TTCCAGGGGC 
GATCAGGAAT CTCCAGATGC TTGTCTGCCT CCTACAGTAC CTGAAGCCCC AGCCCCACCC 
CAAAAGCCCC TTAACTCTCA GAGCCAGAAA CATCTTGCAC CTCCGCCCCT TCTTTCTCCC 
CTTTTACCTT CTATCAAGCC AACCGTTCGT AAGACCAGGC AAGATGGGAG TCAGGAAGCT 
CCAGAGGCTC CCTTGTCCTC AGAGCTGGAG CCTTTCCACC CAAAGCCTAA AATTAGAACT 
CGGAAGTCCT CCAGAATGAC ACCCTTTCCA GCTACCTCTG CTGCCCCTGA GCCCCACCCT 
TCCACCTCCA CAGCCCAGCC AGTCACTCCC AAGCCCACAT CTCAGGCCAC TAGGAGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAA CCAGTTGTCC CCACAGCCCC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCCTGTCACC TCTGAGCCCA CATCTCAGGT TACTAGGGGA 
AGAAAAAGTA GATCCTCTGT CAAGACCCCT GAAACAGTTG TGCCCACAGC CCTTGAGCTC 
CAGCCTTCCA CCTCCACCGA CCGACCTGTC ACCTCTGAAC CCACCTCTCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTCCCCAC AGCCCCTGAG 
CTCCAGCCTT CCACCTCCAC AGACCAGCCT GTCACTTCTG AGCCCACATA TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTGCC CACAGCCCCT 
GAGCTCCGGC CTTCCACCTC CACAGACCGA CCTGTCACCC CCAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTC AAGACCCCTG AAACAGTTGT CCCCACAGCC 
CCTGAGCTCC AGATTTCCAC CTCCACAGAC CAACCTGTCA CCCCTAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTGAAGAACC CTGAATCAAC TGTCCCTATA 
GCCCCTGAGC TCCCACCTTC CACCTCCACA GAGCAGCCTG TCACCCCTGA GCCCACATCT 
CGGGCTACTA GGGGAAGAAA AAATAGATCC TCTGGCAAGA CCCCTGAAAC ACTTGTCCCC 
ACAGCCCCTA AGCTCGAGCC TTCCACTTCC ACAGACCAAC CTGTCACTCC TGAGCCCACA 
TCTCAGGCCA CCAGGGGCAG GACAAATAGG TCCTCTGTGA AGACCCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTTAC CCCTGAGCCT 
ACGTCTCAGG CTACTAGGGG AAGAACAGAT AGATCCTCTG TCAAGACTCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT ACAGGCTTCC GCCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC GGACCACTAG GGGAAGAAAA AATCGGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCG CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAACC TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGGGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAA 
TCAATTGTCC CTATAGCCCC TGAGCTTCAG CCTTCCACCT CCAGAAACCA GCTTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGTGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAGCCAGTTG TCCCCACAGC CCCTGAGCCC CATCCTACCA CCTCCACAGA CCAGCCTGTC 
ACCCCCAAGC TCACATCTAG GGCCACTAGG AGAAAGACAA ATAGGTCCTC TGTCAAGACT 
CCCAAACCAG TTGAACCAGC AGCCTCTGAT CTTGAGCCTT TTACCCCCAC AGACCAGTCC 
GTCACCCCTG AGGCCATAGC TCAGGGTGGT CAGAGCAAAA CACTGAGGTC TTCCACAGTA 
AGAGCTATGC CGGTTCCTAC CACCCCTGAA TTCCAATCTC CTGTCACCAC AGACCAGCCT 
ATTTCCCCTG AGCCTATTAC TCAACCCAGT TGCATCAAGA GGCAGAGAGC CGCTGGGAAC 
CCTGGCTCCC TCGCAGCTCC CATTGACCAT AAGCCTTGCT CTGCACCCTT GGAACCTAAA 
TCCCAGGCCT CAAGGAACCA AAGATGGGGA GCAGTGAGAG CAGCTGAATC CCTTACAGCC 
ATTCCTGAGC CTGCCTCTCC CCAGCTTCTT GAGACACCAA TTCATGCCTC CCAGATCCAA 
AAGGTGGAAC CAGCAGGTAG ATCTAGGTTC ACCCCGGAGC TCCAGCCTAA GGCCTCTCAA 
AGCCGCAAGA GGTCTTTAGC TACCATGGAT TCACCACCAC ATCAAAAACA GCCCCAAAGA 
GGGGAAGTCT CCCAGAAGAC AGTGATTATC AAGGAAGAGG AAGAAGATAC TGCAGAGAAG 
CCAGGGAAGG AAGAGGATGT CGTGACTCCA AAACCAGGCA AGAGAAAGAG AGACCAGGCA 
GAGGAGGAGC CCAACAGAAT ACCAAGCCGC AGCCTCCGAC GGACCAAACT TAACCAAGAA 
TCAACAGCCC CCAAAGTGCT CTTCACAGGA GTGGTGGATG CTCGGGGAGA GCGGGCTGTG 
CTGGCACTGG GGGGAAGTCT GGCTGGTTCA GCGGCAGAGG CTTCCCACCT GGTCACTGAT 
CGCATCCGCC GGACAGTCAA GTTCCTGTGT GCCCTGGGGC GGGGAATCCC CATTCTGTCC 
CTGGACTGGC TGCATCAGTC CCGCAAGGCT GGTTTCTTCT TACCCCCGGA TGAATATGTG 
GTGACCGACC CTGAGCAAGA GAAGAACTTT GGCTTTAGCC TTCAAGACGC ACTGAGCAGG 
GCTCGGGAGC GAAGGCTGCT AGAGGGCTAT GAGATCTATG TGACCCCTGG AGTCCAGCCA 
CCACCACCTC AGATGGGAGA GATTATTAGC TGCTGTGGAG GCACATACCT ACCCAGCATG 
CCTCGGTCCT ATAAGCCTCA GAGAGTTGTG ATCACATGCC CTCAGGACTT CCCTCATTGC 
TCCATTCCAC TACGGGTTGG GCTGCCCCTC CTCTCGCCTG AGTTCCTGCT GACTGGAGTG 
CTGAAGCAGG AAGCCAAGCC AGAGGCCTTT GTCCTCTCCC CTTTGGAGAT GTCATCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249554.1
CDS	282..6044
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249497.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249497.4

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGTACCC TAGATGAACC ATGGGAGGTC CTGGCTACAC AGCCATTCTG TCTGAGAGAG 
TCTGAGGACT CTGAGACCCA GCCTTTTGAC ACGCACCTTG AGGCCTATGG ACCTTGCCTG 
TCTCCACCTA GGGCAATACC AGGAGACCAA CATCCAGAGA GCCCAGTTCA CACAGAGCCA 
ATGGGGATTC AAGGCAGAGG GAGGCAGACT GTGGATAAAG TCATGGGCCT CCTGAATTGC 
AAGATGCCAC CTGCTGAGAA GGCTTCCAGG ATCAGAGCTG CTGAGAAGGT TTCCAGGGGC 
GATCAGGAAT CTCCAGATGC TTGTCTGCCT CCTACAGTAC CTGAAGCCCC AGCCCCACCC 
CAAAAGCCCC TTAACTCTCA GAGCCAGAAA CATCTTGCAC CTCCGCCCCT TCTTTCTCCC 
CTTTTACCTT CTATCAAGCC AACCGTTCGT AAGACCAGGC AAGATGGGAG TCAGGAAGCT 
CCAGAGGCTC CCTTGTCCTC AGAGCTGGAG CCTTTCCACC CAAAGCCTAA AATTAGAACT 
CGGAAGTCCT CCAGAATGAC ACCCTTTCCA GCTACCTCTG CTGCCCCTGA GCCCCACCCT 
TCCACCTCCA CAGCCCAGCC AGTCACTCCC AAGCCCACAT CTCAGGCCAC TAGGAGCAGG 
ACAAATAGGT CCTCTGTCAA GACCCCTGAA CCAGTTGTCC CCACAGCCCC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCCTGTCACC TCTGAGCCCA CATCTCAGGT TACTAGGGGA 
AGAAAAAGTA GATCCTCTGT CAAGACCCCT GAAACAGTTG TGCCCACAGC CCTTGAGCTC 
CAGCCTTCCA CCTCCACCGA CCGACCTGTC ACCTCTGAAC CCACCTCTCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTCCCCAC AGCCCCTGAG 
CTCCAGCCTT CCACCTCCAC AGACCAGCCT GTCACTTCTG AGCCCACATA TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTGCC CACAGCCCCT 
GAGCTCCGGC CTTCCACCTC CACAGACCGA CCTGTCACCC CCAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTC AAGACCCCTG AAACAGTTGT CCCCACAGCC 
CCTGAGCTCC AGATTTCCAC CTCCACAGAC CAACCTGTCA CCCCTAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTGAAGAACC CTGAATCAAC TGTCCCTATA 
GCCCCTGAGC TCCCACCTTC CACCTCCACA GAGCAGCCTG TCACCCCTGA GCCCACATCT 
CGGGCTACTA GGGGAAGAAA AAATAGATCC TCTGGCAAGA CCCCTGAAAC ACTTGTCCCC 
ACAGCCCCTA AGCTCGAGCC TTCCACTTCC ACAGACCAAC CTGTCACTCC TGAGCCCACA 
TCTCAGGCCA CCAGGGGCAG GACAAATAGG TCCTCTGTGA AGACCCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTTAC CCCTGAGCCT 
ACGTCTCAGG CTACTAGGGG AAGAACAGAT AGATCCTCTG TCAAGACTCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT ACAGGCTTCC GCCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC GGACCACTAG GGGAAGAAAA AATCGGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCG CAGCCCCTGA GCTCCAGCCT TCCACCTCCA CAGACCAACC TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGGGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAA 
TCAATTGTCC CTATAGCCCC TGAGCTTCAG CCTTCCACCT CCAGAAACCA GCTTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGTGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAGCCAGTTG TCCCCACAGC CCCTGAGCCC CATCCTACCA CCTCCACAGA CCAGCCTGTC 
ACCCCCAAGC TCACATCTAG GGCCACTAGG AGAAAGACAA ATAGGTCCTC TGTCAAGACT 
CCCAAACCAG TTGAACCAGC AGCCTCTGAT CTTGAGCCTT TTACCCCCAC AGACCAGTCC 
GTCACCCCTG AGGCCATAGC TCAGGGTGGT CAGAGCAAAA CACTGAGGTC TTCCACAGTA 
AGAGCTATGC CGGTTCCTAC CACCCCTGAA TTCCAATCTC CTGTCACCAC AGACCAGCCT 
ATTTCCCCTG AGCCTATTAC TCAACCCAGT TGCATCAAGA GGCAGAGAGC CGCTGGGAAC 
CCTGGCTCCC TCGCAGCTCC CATTGACCAT AAGCCTTGCT CTGCACCCTT GGAACCTAAA 
TCCCAGGCCT CAAGGAACCA AAGATGGGGA GCAGTGAGAG CAGCTGAATC CCTTACAGCC 
ATTCCTGAGC CTGCCTCTCC CCAGCTTCTT GAGACACCAA TTCATGCCTC CCAGATCCAA 
AAGGTGGAAC CAGCAGGTAG ATCTAGGTTC ACCCCGGAGC TCCAGCCTAA GGCCTCTCAA 
AGCCGCAAGA GGTCTTTAGC TACCATGGAT TCACCACCAC ATCAAAAACA GCCCCAAAGA 
GGGGAAGTCT CCCAGAAGAC AGTGATTATC AAGGAAGAGG AAGAAGATAC TGCAGAGAAG 
CCAGGGAAGG AAGAGGATGT CGTGACTCCA AAACCAGGCA AGAGAAAGAG AGACCAGGCA 
GAGGAGGAGC CCAACAGAAT ACCAAGCCGC AGCCTCCGAC GGACCAAACT TAACCAAGAA 
TCAACAGCCC CCAAAGTGCT CTTCACAGGA GTGGTGGATG CTCGGGGAGA GCGGGCTGTG 
CTGGCACTGG GGGGAAGTCT GGCTGGTTCA GCGGCAGAGG CTTCCCACCT GGTCACTGAT 
CGCATCCGCC GGACAGTCAA GTTCCTGTGT GCCCTGGGGC GGGGAATCCC CATTCTGTCC 
CTGGACTGGC TGCATCAGTC CCGCAAGGCT GGTTTCTTCT TACCCCCGGA TGAATATGTG 
GTGACCGACC CTGAGCAAGA GAAGAACTTT GGCTTTAGCC TTCAAGACGC ACTGAGCAGG 
GCTCGGGAGC GAAGGCTGCT AGAGGGCTAT GAGATCTATG TGACCCCTGG AGTCCAGCCA 
CCACCACCTC AGATGGGAGA GATTATTAGC TGCTGTGGAG GCACATACCT ACCCAGCATG 
CCTCGGTCCT ATAAGCCTCA GAGAGTTGTG ATCACATGCC CTCAGGACTT CCCTCATTGC 
TCCATTCCAC TACGGGTTGG GCTGCCCCTC CTCTCGCCTG AGTTCCTGCT GACTGGAGTG 
CTGAAGCAGG AAGCCAAGCC AGAGGCCTTT GTCCTCTCCC CTTTGGAGAT GTCATCCACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249498.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249555.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

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CloneID	OHu40545
Clone ID Related Accession (Same CDS sequence)	XM_005249498.4 , XM_005249498.3
Accession Version	XM_005249498.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005249498.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGCCTCC TGAATTGCAA GATGCCACCT GCTGAGAAGG CTTCCAGGAT CAGAGCTGCT 
GAGAAGGTTT CCAGGGGCGA TCAGGAATCT CCAGATGCTT GTCTGCCTCC TACAGTACCT 
GAAGCCCCAG CCCCACCCCA AAAGCCCCTT AACTCTCAGA GCCAGAAACA TCTTGCACCT 
CCGCCCCTTC TTTCTCCCCT TTTACCTTCT ATCAAGCCAA CCGTTCGTAA GACCAGGCAA 
GATGGGAGTC AGGAAGCTCC AGAGGCTCCC TTGTCCTCAG AGCTGGAGCC TTTCCACCCA 
AAGCCTAAAA TTAGAACTCG GAAGTCCTCC AGAATGACAC CCTTTCCAGC TACCTCTGCT 
GCCCCTGAGC CCCACCCTTC CACCTCCACA GCCCAGCCAG TCACTCCCAA GCCCACATCT 
CAGGCCACTA GGAGCAGGAC AAATAGGTCC TCTGTCAAGA CCCCTGAACC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTCACCTC TGAGCCCACA 
TCTCAGGTTA CTAGGGGAAG AAAAAGTAGA TCCTCTGTCA AGACCCCTGA AACAGTTGTG 
CCCACAGCCC TTGAGCTCCA GCCTTCCACC TCCACCGACC GACCTGTCAC CTCTGAACCC 
ACCTCTCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACTTCTGAG 
CCCACATATC AGGCTACTAG GGGAAGAAAA AATAGATCCT CTGTCAAGAC CCCTGAACCA 
GTTGTGCCCA CAGCCCCTGA GCTCCGGCCT TCCACCTCCA CAGACCGACC TGTCACCCCC 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTCC CCACAGCCCC TGAGCTCCAG ATTTCCACCT CCACAGACCA ACCTGTCACC 
CCTAAGCCCA CATCTCGGAC CACTAGGAGC AGGACAAATA TGTCCTCTGT GAAGAACCCT 
GAATCAACTG TCCCTATAGC CCCTGAGCTC CCACCTTCCA CCTCCACAGA GCAGCCTGTC 
ACCCCTGAGC CCACATCTCG GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGGCAAGACC 
CCTGAAACAC TTGTCCCCAC AGCCCCTAAG CTCGAGCCTT CCACTTCCAC AGACCAACCT 
GTCACTCCTG AGCCCACATC TCAGGCCACC AGGGGCAGGA CAAATAGGTC CTCTGTGAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGC CTTCCACCTC CACAGACCAG 
CCTGTTACCC CTGAGCCTAC GTCTCAGGCT ACTAGGGGAA GAACAGATAG ATCCTCTGTC 
AAGACTCCTG AAACAGTTGT CCCCACAGCC CCTGAGCTAC AGGCTTCCGC CTCCACAGAC 
CAGCCTGTCA CCTCTGAGCC CACATCTCGG ACCACTAGGG GAAGAAAAAA TCGGTCCTCT 
GTCAAGACCC CTGAAACAGT TGTGCCCGCA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAACCTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGGGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAATC AATTGTCCCT ATAGCCCCTG AGCTTCAGCC TTCCACCTCC 
AGAAACCAGC TTGTCACCCC TGAGCCCACA TCTCGGGCCA CTAGGTGCAG GACAAATAGG 
TCCTCTGTCA AGACCCCTGA GCCAGTTGTC CCCACAGCCC CTGAGCCCCA TCCTACCACC 
TCCACAGACC AGCCTGTCAC CCCCAAGCTC ACATCTAGGG CCACTAGGAG AAAGACAAAT 
AGGTCCTCTG TCAAGACTCC CAAACCAGTT GAACCAGCAG CCTCTGATCT TGAGCCTTTT 
ACCCCCACAG ACCAGTCCGT CACCCCTGAG GCCATAGCTC AGGGTGGTCA GAGCAAAACA 
CTGAGGTCTT CCACAGTAAG AGCTATGCCG GTTCCTACCA CCCCTGAATT CCAATCTCCT 
GTCACCACAG ACCAGCCTAT TTCCCCTGAG CCTATTACTC AACCCAGTTG CATCAAGAGG 
CAGAGAGCCG CTGGGAACCC TGGCTCCCTC GCAGCTCCCA TTGACCATAA GCCTTGCTCT 
GCACCCTTGG AACCTAAATC CCAGGCCTCA AGGAACCAAA GATGGGGAGC AGTGAGAGCA 
GCTGAATCCC TTACAGCCAT TCCTGAGCCT GCCTCTCCCC AGCTTCTTGA GACACCAATT 
CATGCCTCCC AGATCCAAAA GGTGGAACCA GCAGGTAGAT CTAGGTTCAC CCCGGAGCTC 
CAGCCTAAGG CCTCTCAAAG CCGCAAGAGG TCTTTAGCTA CCATGGATTC ACCACCACAT 
CAAAAACAGC CCCAAAGAGG GGAAGTCTCC CAGAAGACAG TGATTATCAA GGAAGAGGAA 
GAAGATACTG CAGAGAAGCC AGGGAAGGAA GAGGATGTCG TGACTCCAAA ACCAGGCAAG 
AGAAAGAGAG ACCAGGCAGA GGAGGAGCCC AACAGAATAC CAAGCCGCAG CCTCCGACGG 
ACCAAACTTA ACCAAGAATC AACAGCCCCC AAAGTGCTCT TCACAGGAGT GGTGGATGCT 
CGGGGAGAGC GGGCTGTGCT GGCACTGGGG GGAAGTCTGG CTGGTTCAGC GGCAGAGGCT 
TCCCACCTGG TCACTGATCG CATCCGCCGG ACAGTCAAGT TCCTGTGTGC CCTGGGGCGG 
GGAATCCCCA TTCTGTCCCT GGACTGGCTG CATCAGTCCC GCAAGGCTGG TTTCTTCTTA 
CCCCCGGATG AATATGTGGT GACCGACCCT GAGCAAGAGA AGAACTTTGG CTTTAGCCTT 
CAAGACGCAC TGAGCAGGGC TCGGGAGCGA AGGCTGCTAG AGGGCTATGA GATCTATGTG 
ACCCCTGGAG TCCAGCCACC ACCACCTCAG ATGGGAGAGA TTATTAGCTG CTGTGGAGGC 
ACATACCTAC CCAGCATGCC TCGGTCCTAT AAGCCTCAGA GAGTTGTGAT CACATGCCCT 
CAGGACTTCC CTCATTGCTC CATTCCACTA CGGGTTGGGC TGCCCCTCCT CTCGCCTGAG 
TTCCTGCTGA CTGGAGTGCT GAAGCAGGAA GCCAAGCCAG AGGCCTTTGT CCTCTCCCCT 
TTGGAGATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249555.1
CDS	282..5822
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249498.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249498.4

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGCCTCC TGAATTGCAA GATGCCACCT GCTGAGAAGG CTTCCAGGAT CAGAGCTGCT 
GAGAAGGTTT CCAGGGGCGA TCAGGAATCT CCAGATGCTT GTCTGCCTCC TACAGTACCT 
GAAGCCCCAG CCCCACCCCA AAAGCCCCTT AACTCTCAGA GCCAGAAACA TCTTGCACCT 
CCGCCCCTTC TTTCTCCCCT TTTACCTTCT ATCAAGCCAA CCGTTCGTAA GACCAGGCAA 
GATGGGAGTC AGGAAGCTCC AGAGGCTCCC TTGTCCTCAG AGCTGGAGCC TTTCCACCCA 
AAGCCTAAAA TTAGAACTCG GAAGTCCTCC AGAATGACAC CCTTTCCAGC TACCTCTGCT 
GCCCCTGAGC CCCACCCTTC CACCTCCACA GCCCAGCCAG TCACTCCCAA GCCCACATCT 
CAGGCCACTA GGAGCAGGAC AAATAGGTCC TCTGTCAAGA CCCCTGAACC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTCACCTC TGAGCCCACA 
TCTCAGGTTA CTAGGGGAAG AAAAAGTAGA TCCTCTGTCA AGACCCCTGA AACAGTTGTG 
CCCACAGCCC TTGAGCTCCA GCCTTCCACC TCCACCGACC GACCTGTCAC CTCTGAACCC 
ACCTCTCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACTTCTGAG 
CCCACATATC AGGCTACTAG GGGAAGAAAA AATAGATCCT CTGTCAAGAC CCCTGAACCA 
GTTGTGCCCA CAGCCCCTGA GCTCCGGCCT TCCACCTCCA CAGACCGACC TGTCACCCCC 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTCC CCACAGCCCC TGAGCTCCAG ATTTCCACCT CCACAGACCA ACCTGTCACC 
CCTAAGCCCA CATCTCGGAC CACTAGGAGC AGGACAAATA TGTCCTCTGT GAAGAACCCT 
GAATCAACTG TCCCTATAGC CCCTGAGCTC CCACCTTCCA CCTCCACAGA GCAGCCTGTC 
ACCCCTGAGC CCACATCTCG GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGGCAAGACC 
CCTGAAACAC TTGTCCCCAC AGCCCCTAAG CTCGAGCCTT CCACTTCCAC AGACCAACCT 
GTCACTCCTG AGCCCACATC TCAGGCCACC AGGGGCAGGA CAAATAGGTC CTCTGTGAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGC CTTCCACCTC CACAGACCAG 
CCTGTTACCC CTGAGCCTAC GTCTCAGGCT ACTAGGGGAA GAACAGATAG ATCCTCTGTC 
AAGACTCCTG AAACAGTTGT CCCCACAGCC CCTGAGCTAC AGGCTTCCGC CTCCACAGAC 
CAGCCTGTCA CCTCTGAGCC CACATCTCGG ACCACTAGGG GAAGAAAAAA TCGGTCCTCT 
GTCAAGACCC CTGAAACAGT TGTGCCCGCA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAACCTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGGGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAATC AATTGTCCCT ATAGCCCCTG AGCTTCAGCC TTCCACCTCC 
AGAAACCAGC TTGTCACCCC TGAGCCCACA TCTCGGGCCA CTAGGTGCAG GACAAATAGG 
TCCTCTGTCA AGACCCCTGA GCCAGTTGTC CCCACAGCCC CTGAGCCCCA TCCTACCACC 
TCCACAGACC AGCCTGTCAC CCCCAAGCTC ACATCTAGGG CCACTAGGAG AAAGACAAAT 
AGGTCCTCTG TCAAGACTCC CAAACCAGTT GAACCAGCAG CCTCTGATCT TGAGCCTTTT 
ACCCCCACAG ACCAGTCCGT CACCCCTGAG GCCATAGCTC AGGGTGGTCA GAGCAAAACA 
CTGAGGTCTT CCACAGTAAG AGCTATGCCG GTTCCTACCA CCCCTGAATT CCAATCTCCT 
GTCACCACAG ACCAGCCTAT TTCCCCTGAG CCTATTACTC AACCCAGTTG CATCAAGAGG 
CAGAGAGCCG CTGGGAACCC TGGCTCCCTC GCAGCTCCCA TTGACCATAA GCCTTGCTCT 
GCACCCTTGG AACCTAAATC CCAGGCCTCA AGGAACCAAA GATGGGGAGC AGTGAGAGCA 
GCTGAATCCC TTACAGCCAT TCCTGAGCCT GCCTCTCCCC AGCTTCTTGA GACACCAATT 
CATGCCTCCC AGATCCAAAA GGTGGAACCA GCAGGTAGAT CTAGGTTCAC CCCGGAGCTC 
CAGCCTAAGG CCTCTCAAAG CCGCAAGAGG TCTTTAGCTA CCATGGATTC ACCACCACAT 
CAAAAACAGC CCCAAAGAGG GGAAGTCTCC CAGAAGACAG TGATTATCAA GGAAGAGGAA 
GAAGATACTG CAGAGAAGCC AGGGAAGGAA GAGGATGTCG TGACTCCAAA ACCAGGCAAG 
AGAAAGAGAG ACCAGGCAGA GGAGGAGCCC AACAGAATAC CAAGCCGCAG CCTCCGACGG 
ACCAAACTTA ACCAAGAATC AACAGCCCCC AAAGTGCTCT TCACAGGAGT GGTGGATGCT 
CGGGGAGAGC GGGCTGTGCT GGCACTGGGG GGAAGTCTGG CTGGTTCAGC GGCAGAGGCT 
TCCCACCTGG TCACTGATCG CATCCGCCGG ACAGTCAAGT TCCTGTGTGC CCTGGGGCGG 
GGAATCCCCA TTCTGTCCCT GGACTGGCTG CATCAGTCCC GCAAGGCTGG TTTCTTCTTA 
CCCCCGGATG AATATGTGGT GACCGACCCT GAGCAAGAGA AGAACTTTGG CTTTAGCCTT 
CAAGACGCAC TGAGCAGGGC TCGGGAGCGA AGGCTGCTAG AGGGCTATGA GATCTATGTG 
ACCCCTGGAG TCCAGCCACC ACCACCTCAG ATGGGAGAGA TTATTAGCTG CTGTGGAGGC 
ACATACCTAC CCAGCATGCC TCGGTCCTAT AAGCCTCAGA GAGTTGTGAT CACATGCCCT 
CAGGACTTCC CTCATTGCTC CATTCCACTA CGGGTTGGGC TGCCCCTCCT CTCGCCTGAG 
TTCCTGCTGA CTGGAGTGCT GAAGCAGGAA GCCAAGCCAG AGGCCTTTGT CCTCTCCCCT 
TTGGAGATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_005249498.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249555.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

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CloneID	OHu40545
Clone ID Related Accession (Same CDS sequence)	XM_005249498.4 , XM_005249498.3
Accession Version	XM_005249498.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5541bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249498.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGCCTCC TGAATTGCAA GATGCCACCT GCTGAGAAGG CTTCCAGGAT CAGAGCTGCT 
GAGAAGGTTT CCAGGGGCGA TCAGGAATCT CCAGATGCTT GTCTGCCTCC TACAGTACCT 
GAAGCCCCAG CCCCACCCCA AAAGCCCCTT AACTCTCAGA GCCAGAAACA TCTTGCACCT 
CCGCCCCTTC TTTCTCCCCT TTTACCTTCT ATCAAGCCAA CCGTTCGTAA GACCAGGCAA 
GATGGGAGTC AGGAAGCTCC AGAGGCTCCC TTGTCCTCAG AGCTGGAGCC TTTCCACCCA 
AAGCCTAAAA TTAGAACTCG GAAGTCCTCC AGAATGACAC CCTTTCCAGC TACCTCTGCT 
GCCCCTGAGC CCCACCCTTC CACCTCCACA GCCCAGCCAG TCACTCCCAA GCCCACATCT 
CAGGCCACTA GGAGCAGGAC AAATAGGTCC TCTGTCAAGA CCCCTGAACC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTCACCTC TGAGCCCACA 
TCTCAGGTTA CTAGGGGAAG AAAAAGTAGA TCCTCTGTCA AGACCCCTGA AACAGTTGTG 
CCCACAGCCC TTGAGCTCCA GCCTTCCACC TCCACCGACC GACCTGTCAC CTCTGAACCC 
ACCTCTCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACTTCTGAG 
CCCACATATC AGGCTACTAG GGGAAGAAAA AATAGATCCT CTGTCAAGAC CCCTGAACCA 
GTTGTGCCCA CAGCCCCTGA GCTCCGGCCT TCCACCTCCA CAGACCGACC TGTCACCCCC 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTCC CCACAGCCCC TGAGCTCCAG ATTTCCACCT CCACAGACCA ACCTGTCACC 
CCTAAGCCCA CATCTCGGAC CACTAGGAGC AGGACAAATA TGTCCTCTGT GAAGAACCCT 
GAATCAACTG TCCCTATAGC CCCTGAGCTC CCACCTTCCA CCTCCACAGA GCAGCCTGTC 
ACCCCTGAGC CCACATCTCG GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGGCAAGACC 
CCTGAAACAC TTGTCCCCAC AGCCCCTAAG CTCGAGCCTT CCACTTCCAC AGACCAACCT 
GTCACTCCTG AGCCCACATC TCAGGCCACC AGGGGCAGGA CAAATAGGTC CTCTGTGAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGC CTTCCACCTC CACAGACCAG 
CCTGTTACCC CTGAGCCTAC GTCTCAGGCT ACTAGGGGAA GAACAGATAG ATCCTCTGTC 
AAGACTCCTG AAACAGTTGT CCCCACAGCC CCTGAGCTAC AGGCTTCCGC CTCCACAGAC 
CAGCCTGTCA CCTCTGAGCC CACATCTCGG ACCACTAGGG GAAGAAAAAA TCGGTCCTCT 
GTCAAGACCC CTGAAACAGT TGTGCCCGCA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAACCTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGGGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAATC AATTGTCCCT ATAGCCCCTG AGCTTCAGCC TTCCACCTCC 
AGAAACCAGC TTGTCACCCC TGAGCCCACA TCTCGGGCCA CTAGGTGCAG GACAAATAGG 
TCCTCTGTCA AGACCCCTGA GCCAGTTGTC CCCACAGCCC CTGAGCCCCA TCCTACCACC 
TCCACAGACC AGCCTGTCAC CCCCAAGCTC ACATCTAGGG CCACTAGGAG AAAGACAAAT 
AGGTCCTCTG TCAAGACTCC CAAACCAGTT GAACCAGCAG CCTCTGATCT TGAGCCTTTT 
ACCCCCACAG ACCAGTCCGT CACCCCTGAG GCCATAGCTC AGGGTGGTCA GAGCAAAACA 
CTGAGGTCTT CCACAGTAAG AGCTATGCCG GTTCCTACCA CCCCTGAATT CCAATCTCCT 
GTCACCACAG ACCAGCCTAT TTCCCCTGAG CCTATTACTC AACCCAGTTG CATCAAGAGG 
CAGAGAGCCG CTGGGAACCC TGGCTCCCTC GCAGCTCCCA TTGACCATAA GCCTTGCTCT 
GCACCCTTGG AACCTAAATC CCAGGCCTCA AGGAACCAAA GATGGGGAGC AGTGAGAGCA 
GCTGAATCCC TTACAGCCAT TCCTGAGCCT GCCTCTCCCC AGCTTCTTGA GACACCAATT 
CATGCCTCCC AGATCCAAAA GGTGGAACCA GCAGGTAGAT CTAGGTTCAC CCCGGAGCTC 
CAGCCTAAGG CCTCTCAAAG CCGCAAGAGG TCTTTAGCTA CCATGGATTC ACCACCACAT 
CAAAAACAGC CCCAAAGAGG GGAAGTCTCC CAGAAGACAG TGATTATCAA GGAAGAGGAA 
GAAGATACTG CAGAGAAGCC AGGGAAGGAA GAGGATGTCG TGACTCCAAA ACCAGGCAAG 
AGAAAGAGAG ACCAGGCAGA GGAGGAGCCC AACAGAATAC CAAGCCGCAG CCTCCGACGG 
ACCAAACTTA ACCAAGAATC AACAGCCCCC AAAGTGCTCT TCACAGGAGT GGTGGATGCT 
CGGGGAGAGC GGGCTGTGCT GGCACTGGGG GGAAGTCTGG CTGGTTCAGC GGCAGAGGCT 
TCCCACCTGG TCACTGATCG CATCCGCCGG ACAGTCAAGT TCCTGTGTGC CCTGGGGCGG 
GGAATCCCCA TTCTGTCCCT GGACTGGCTG CATCAGTCCC GCAAGGCTGG TTTCTTCTTA 
CCCCCGGATG AATATGTGGT GACCGACCCT GAGCAAGAGA AGAACTTTGG CTTTAGCCTT 
CAAGACGCAC TGAGCAGGGC TCGGGAGCGA AGGCTGCTAG AGGGCTATGA GATCTATGTG 
ACCCCTGGAG TCCAGCCACC ACCACCTCAG ATGGGAGAGA TTATTAGCTG CTGTGGAGGC 
ACATACCTAC CCAGCATGCC TCGGTCCTAT AAGCCTCAGA GAGTTGTGAT CACATGCCCT 
CAGGACTTCC CTCATTGCTC CATTCCACTA CGGGTTGGGC TGCCCCTCCT CTCGCCTGAG 
TTCCTGCTGA CTGGAGTGCT GAAGCAGGAA GCCAAGCCAG AGGCCTTTGT CCTCTCCCCT 
TTGGAGATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249555.1
CDS	282..5822
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_005249498.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249498.3

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GCAGTCCAGA GCATGGAGGA TGAACCTACC 
CAGGCCTTCA TGTTGACTCC ACCCCAAGAG CTTGGCCCTT CCCATTGCAG CTTCCAGACA 
ACAGGCCTCC TGAATTGCAA GATGCCACCT GCTGAGAAGG CTTCCAGGAT CAGAGCTGCT 
GAGAAGGTTT CCAGGGGCGA TCAGGAATCT CCAGATGCTT GTCTGCCTCC TACAGTACCT 
GAAGCCCCAG CCCCACCCCA AAAGCCCCTT AACTCTCAGA GCCAGAAACA TCTTGCACCT 
CCGCCCCTTC TTTCTCCCCT TTTACCTTCT ATCAAGCCAA CCGTTCGTAA GACCAGGCAA 
GATGGGAGTC AGGAAGCTCC AGAGGCTCCC TTGTCCTCAG AGCTGGAGCC TTTCCACCCA 
AAGCCTAAAA TTAGAACTCG GAAGTCCTCC AGAATGACAC CCTTTCCAGC TACCTCTGCT 
GCCCCTGAGC CCCACCCTTC CACCTCCACA GCCCAGCCAG TCACTCCCAA GCCCACATCT 
CAGGCCACTA GGAGCAGGAC AAATAGGTCC TCTGTCAAGA CCCCTGAACC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTCACCTC TGAGCCCACA 
TCTCAGGTTA CTAGGGGAAG AAAAAGTAGA TCCTCTGTCA AGACCCCTGA AACAGTTGTG 
CCCACAGCCC TTGAGCTCCA GCCTTCCACC TCCACCGACC GACCTGTCAC CTCTGAACCC 
ACCTCTCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACTTCTGAG 
CCCACATATC AGGCTACTAG GGGAAGAAAA AATAGATCCT CTGTCAAGAC CCCTGAACCA 
GTTGTGCCCA CAGCCCCTGA GCTCCGGCCT TCCACCTCCA CAGACCGACC TGTCACCCCC 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTCC CCACAGCCCC TGAGCTCCAG ATTTCCACCT CCACAGACCA ACCTGTCACC 
CCTAAGCCCA CATCTCGGAC CACTAGGAGC AGGACAAATA TGTCCTCTGT GAAGAACCCT 
GAATCAACTG TCCCTATAGC CCCTGAGCTC CCACCTTCCA CCTCCACAGA GCAGCCTGTC 
ACCCCTGAGC CCACATCTCG GGCTACTAGG GGAAGAAAAA ATAGATCCTC TGGCAAGACC 
CCTGAAACAC TTGTCCCCAC AGCCCCTAAG CTCGAGCCTT CCACTTCCAC AGACCAACCT 
GTCACTCCTG AGCCCACATC TCAGGCCACC AGGGGCAGGA CAAATAGGTC CTCTGTGAAG 
ACCCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTCCAGC CTTCCACCTC CACAGACCAG 
CCTGTTACCC CTGAGCCTAC GTCTCAGGCT ACTAGGGGAA GAACAGATAG ATCCTCTGTC 
AAGACTCCTG AAACAGTTGT CCCCACAGCC CCTGAGCTAC AGGCTTCCGC CTCCACAGAC 
CAGCCTGTCA CCTCTGAGCC CACATCTCGG ACCACTAGGG GAAGAAAAAA TCGGTCCTCT 
GTCAAGACCC CTGAAACAGT TGTGCCCGCA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAACCTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGGGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAATC AATTGTCCCT ATAGCCCCTG AGCTTCAGCC TTCCACCTCC 
AGAAACCAGC TTGTCACCCC TGAGCCCACA TCTCGGGCCA CTAGGTGCAG GACAAATAGG 
TCCTCTGTCA AGACCCCTGA GCCAGTTGTC CCCACAGCCC CTGAGCCCCA TCCTACCACC 
TCCACAGACC AGCCTGTCAC CCCCAAGCTC ACATCTAGGG CCACTAGGAG AAAGACAAAT 
AGGTCCTCTG TCAAGACTCC CAAACCAGTT GAACCAGCAG CCTCTGATCT TGAGCCTTTT 
ACCCCCACAG ACCAGTCCGT CACCCCTGAG GCCATAGCTC AGGGTGGTCA GAGCAAAACA 
CTGAGGTCTT CCACAGTAAG AGCTATGCCG GTTCCTACCA CCCCTGAATT CCAATCTCCT 
GTCACCACAG ACCAGCCTAT TTCCCCTGAG CCTATTACTC AACCCAGTTG CATCAAGAGG 
CAGAGAGCCG CTGGGAACCC TGGCTCCCTC GCAGCTCCCA TTGACCATAA GCCTTGCTCT 
GCACCCTTGG AACCTAAATC CCAGGCCTCA AGGAACCAAA GATGGGGAGC AGTGAGAGCA 
GCTGAATCCC TTACAGCCAT TCCTGAGCCT GCCTCTCCCC AGCTTCTTGA GACACCAATT 
CATGCCTCCC AGATCCAAAA GGTGGAACCA GCAGGTAGAT CTAGGTTCAC CCCGGAGCTC 
CAGCCTAAGG CCTCTCAAAG CCGCAAGAGG TCTTTAGCTA CCATGGATTC ACCACCACAT 
CAAAAACAGC CCCAAAGAGG GGAAGTCTCC CAGAAGACAG TGATTATCAA GGAAGAGGAA 
GAAGATACTG CAGAGAAGCC AGGGAAGGAA GAGGATGTCG TGACTCCAAA ACCAGGCAAG 
AGAAAGAGAG ACCAGGCAGA GGAGGAGCCC AACAGAATAC CAAGCCGCAG CCTCCGACGG 
ACCAAACTTA ACCAAGAATC AACAGCCCCC AAAGTGCTCT TCACAGGAGT GGTGGATGCT 
CGGGGAGAGC GGGCTGTGCT GGCACTGGGG GGAAGTCTGG CTGGTTCAGC GGCAGAGGCT 
TCCCACCTGG TCACTGATCG CATCCGCCGG ACAGTCAAGT TCCTGTGTGC CCTGGGGCGG 
GGAATCCCCA TTCTGTCCCT GGACTGGCTG CATCAGTCCC GCAAGGCTGG TTTCTTCTTA 
CCCCCGGATG AATATGTGGT GACCGACCCT GAGCAAGAGA AGAACTTTGG CTTTAGCCTT 
CAAGACGCAC TGAGCAGGGC TCGGGAGCGA AGGCTGCTAG AGGGCTATGA GATCTATGTG 
ACCCCTGGAG TCCAGCCACC ACCACCTCAG ATGGGAGAGA TTATTAGCTG CTGTGGAGGC 
ACATACCTAC CCAGCATGCC TCGGTCCTAT AAGCCTCAGA GAGTTGTGAT CACATGCCCT 
CAGGACTTCC CTCATTGCTC CATTCCACTA CGGGTTGGGC TGCCCCTCCT CTCGCCTGAG 
TTCCTGCTGA CTGGAGTGCT GAAGCAGGAA GCCAAGCCAG AGGCCTTTGT CCTCTCCCCT 
TTGGAGATGT CATCCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011520.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867009.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OHu55010
Clone ID Related Accession (Same CDS sequence)	XM_017011520.1 , XM_017011520.2
Accession Version	XM_017011520.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGTC CAGAGCATGG AGGATGAACC TACCCAGGCC 
TTCATGTTGA CTCCACCCCA AGAGCTTGGC CCTTCCCATT GCAGCTTCCA GACAACAGGT 
ACCCTAGATG AACCATGGGA GGTCCTGGCT ACACAGCCAT TCTGTCTGAG AGAGTCTGAG 
GACTCTGAGA CCCAGCCTTT TGACACGCAC CTTGAGGCCT ATGGACCTTG CCTGTCTCCA 
CCTAGGGCAA TACCAGGAGA CCAACATCCA GAGAGCCCAG TTCACACAGA GCCAATGGGG 
ATTCAAGGCA GAGGGAGGCA GACTGTGGAT AAAGTCATGG GTATACCAAA AGAAACAGCA 
GAGAGGGTGG GCCCTGAGAG AGGGCCATTG GAGAGAGAAA CTGAGAAACT GCTACCAGAA 
AGACAGACAG ATGTGACAGG AGAGGAAGAA TTAACCAAGG GGAAACAGGA CAGAGAACAA 
AAACAGTTGT TAGCTAGAGA CACCCAGAGA CAAGAATCTG ACAAAAATGG GGAAAGTGCA 
AGTCCTGAAA GAGATAGGGA GAGTTTGAAG GTAGAAATTG AGACATCTGA GGAAATACAA 
GAGAAACAAG TACAGAAGCA GACCCTTCCA AGCAAAGCAT TTGAGAGAGA AGTAGAGAGA 
CCAGTAGCAA ACAGAGAGTG CGATCCAGCC GAGTTAGAAG AGAAGGTGCC CAAAGTGATC 
CTGGAGAGAG ATACACAGAG AGGGGAGCCA GAGGGAGGGA GCCAGGACCA GAAAGGGCAG 
GCCTCCAGCC CAACACCAGA GCCTGGGGTG GGGGCGGGGG ACCTTCCGGG ACCTACCTCA 
GCCCCCGTAC CTTCTGGGAG CCAGTCAGGT GGAAGGGGAT CCCCAGTGAG CCCCAGGAGG 
CATCAGAAAG GCCTCCTGAA TTGCAAGATG CCACCTGCTG AGAAGGCTTC CAGGATCAGA 
GCTGCTGAGA AGGTTTCCAG GGGCGATCAG GAATCTCCAG ATGCTTGTCT GCCTCCTACA 
GTACCTGAAG CCCCAGCCCC ACCCCAAAAG CCCCTTAACT CTCAGAGCCA GAAACATCTT 
GCACCTCCGC CCCTTCTTTC TCCCCTTTTA CCTTCTATCA AGCCAACCGT TCGTAAGACC 
AGGCAAGATG GGAGTCAGGA AGCTCCAGAG GCTCCCTTGT CCTCAGAGCT GGAGCCTTTC 
CACCCAAAGC CTAAAATTAG AACTCGGAAG TCCTCCAGAA TGACACCCTT TCCAGCTACC 
TCTGCTGCCC CTGAGCCCCA CCCTTCCACC TCCACAGCCC AGCCAGTCAC TCCCAAGCCC 
ACATCTCAGG CCACTAGGAG CAGGACAAAT AGGTCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC AGGTTACTAG GGGAAGAAAA AGTAGATCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCA CAGCCCTTGA GCTCCAGCCT TCCACCTCCA CCGACCGACC TGTCACCTCT 
GAACCCACCT CTCAGGCTAC TAGGGGAAGA AAAAATAGAT CCTCTGTCAA GACCCCTGAA 
CCAGTTGTCC CCACAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCCTGTCACT 
TCTGAGCCCA CATATCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TGCCCACAGC CCCTGAGCTC CGGCCTTCCA CCTCCACAGA CCGACCTGTC 
ACCCCCAAGC CCACATCTCG GACCACTAGG AGCAGGACAA ATATGTCCTC TGTCAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGATTT CCACCTCCAC AGACCAACCT 
GTCACCCCTA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTGAAG 
AACCCTGAAT CAACTGTCCC TATAGCCCCT GAGCTCCCAC CTTCCACCTC CACAGAGCAG 
CCTGTCACCC CTGAGCCCAC ATCTCGGGCT ACTAGGGGAA GAAAAAATAG ATCCTCTGGC 
AAGACCCCTG AAACACTTGT CCCCACAGCC CCTAAGCTCG AGCCTTCCAC TTCCACAGAC 
CAACCTGTCA CTCCTGAGCC CACATCTCAG GCCACCAGGG GCAGGACAAA TAGGTCCTCT 
GTGAAGACCC CTGAAACAGT TGTCCCCACA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAGCCTG TTACCCCTGA GCCTACGTCT CAGGCTACTA GGGGAAGAAC AGATAGATCC 
TCTGTCAAGA CTCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTACAGGC TTCCGCCTCC 
ACAGACCAGC CTGTCACCTC TGAGCCCACA TCTCGGACCA CTAGGGGAAG AAAAAATCGG 
TCCTCTGTCA AGACCCCTGA AACAGTTGTG CCCGCAGCCC CTGAGCTCCA GCCTTCCACC 
TCCACAGACC AACCTGTCAC CCCTGAGCCC ACATCTCGGG CCACTAGGGG CAGGACAAAT 
AGGTCCTCTG TCAAGACCCC TGAATCAATT GTCCCTATAG CCCCTGAGCT TCAGCCTTCC 
ACCTCCAGAA ACCAGCTTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GTGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAGCCA GTTGTCCCCA CAGCCCCTGA GCCCCATCCT 
ACCACCTCCA CAGACCAGCC TGTCACCCCC AAGCTCACAT CTAGGGCCAC TAGGAGAAAG 
ACAAATAGGT CCTCTGTCAA GACTCCCAAA CCAGTTGAAC CAGCAGCCTC TGATCTTGAG 
CCTTTTACCC CCACAGACCA GTCCGTCACC CCTGAGGCCA TAGCTCAGGG TGGTCAGAGC 
AAAACACTGA GGTCTTCCAC AGTAAGAGCT ATGCCGGTTC CTACCACCCC TGAATTCCAA 
TCTCCTGTCA CCACAGACCA GCCTATTTCC CCTGAGCCTA TTACTCAACC CAGTTGCATC 
AAGAGGCAGA GAGCCGCTGG GAACCCTGGC TCCCTCGCAG CTCCCATTGA CCATAAGCCT 
TGCTCTGCAC CCTTGGAACC TAAATCCCAG GCCTCAAGGA ACCAAAGATG GGGAGCAGTG 
AGAGCAGCTG AATCCCTTAC AGCCATTCCT GAGCCTGCCT CTCCCCAGCT TCTTGAGACA 
CCAATTCATG CCTCCCAGAT CCAAAAGGTG GAACCAGCAG GTAGATCTAG GTTCACCCCG 
GAGCTCCAGC CTAAGGCCTC TCAAAGCCGC AAGAGGTCTT TAGCTACCAT GGATTCACCA 
CCACATCAAA AACAGCCCCA AAGAGGGGAA GTCTCCCAGA AGACAGTGAT TATCAAGGAA 
GAGGAAGAAG ATACTGCAGA GAAGCCAGGG AAGGAAGAGG ATGTCGTGAC TCCAAAACCA 
GGCAAGAGAA AGAGAGACCA GGCAGAGGAG GAGCCCAACA GAATACCAAG CCGCAGCCTC 
CGACGGACCA AACTTAACCA AGAATCAACA GCCCCCAAAG TGCTCTTCAC AGGAGTGGTG 
GATGCTCGGG GAGAGCGGGC TGTGCTGGCA CTGGGGGGAA GTCTGGCTGG TTCAGCGGCA 
GAGGCTTCCC ACCTGGTCAC TGATCGCATC CGCCGGACAG TCAAGTTCCT GTGTGCCCTG 
GGGCGGGGAA TCCCCATTCT GTCCCTGGAC TGGCTGCATC AGTCCCGCAA GGCTGGTTTC 
TTCTTACCCC CGGATGAATA TGTGGTGACC GACCCTGAGC AAGAGAAGAA CTTTGGCTTT 
AGCCTTCAAG ACGCACTGAG CAGGGCTCGG GAGCGAAGGC TGCTAGAGGG CTATGAGATC 
TATGTGACCC CTGGAGTCCA GCCACCACCA CCTCAGATGG GAGAGATTAT TAGCTGCTGT 
GGAGGCACAT ACCTACCCAG CATGCCTCGG TCCTATAAGC CTCAGAGAGT TGTGATCACA 
TGCCCTCAGG ACTTCCCTCA TTGCTCCATT CCACTACGGG TTGGGCTGCC CCTCCTCTCG 
CCTGAGTTCC TGCTGACTGG AGTGCTGAAG CAGGAAGCCA AGCCAGAGGC CTTTGTCCTC 
TCCCCTTTGG AGATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867009.1
CDS	43..6309
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011520.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011520.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGTC CAGAGCATGG AGGATGAACC TACCCAGGCC 
TTCATGTTGA CTCCACCCCA AGAGCTTGGC CCTTCCCATT GCAGCTTCCA GACAACAGGT 
ACCCTAGATG AACCATGGGA GGTCCTGGCT ACACAGCCAT TCTGTCTGAG AGAGTCTGAG 
GACTCTGAGA CCCAGCCTTT TGACACGCAC CTTGAGGCCT ATGGACCTTG CCTGTCTCCA 
CCTAGGGCAA TACCAGGAGA CCAACATCCA GAGAGCCCAG TTCACACAGA GCCAATGGGG 
ATTCAAGGCA GAGGGAGGCA GACTGTGGAT AAAGTCATGG GTATACCAAA AGAAACAGCA 
GAGAGGGTGG GCCCTGAGAG AGGGCCATTG GAGAGAGAAA CTGAGAAACT GCTACCAGAA 
AGACAGACAG ATGTGACAGG AGAGGAAGAA TTAACCAAGG GGAAACAGGA CAGAGAACAA 
AAACAGTTGT TAGCTAGAGA CACCCAGAGA CAAGAATCTG ACAAAAATGG GGAAAGTGCA 
AGTCCTGAAA GAGATAGGGA GAGTTTGAAG GTAGAAATTG AGACATCTGA GGAAATACAA 
GAGAAACAAG TACAGAAGCA GACCCTTCCA AGCAAAGCAT TTGAGAGAGA AGTAGAGAGA 
CCAGTAGCAA ACAGAGAGTG CGATCCAGCC GAGTTAGAAG AGAAGGTGCC CAAAGTGATC 
CTGGAGAGAG ATACACAGAG AGGGGAGCCA GAGGGAGGGA GCCAGGACCA GAAAGGGCAG 
GCCTCCAGCC CAACACCAGA GCCTGGGGTG GGGGCGGGGG ACCTTCCGGG ACCTACCTCA 
GCCCCCGTAC CTTCTGGGAG CCAGTCAGGT GGAAGGGGAT CCCCAGTGAG CCCCAGGAGG 
CATCAGAAAG GCCTCCTGAA TTGCAAGATG CCACCTGCTG AGAAGGCTTC CAGGATCAGA 
GCTGCTGAGA AGGTTTCCAG GGGCGATCAG GAATCTCCAG ATGCTTGTCT GCCTCCTACA 
GTACCTGAAG CCCCAGCCCC ACCCCAAAAG CCCCTTAACT CTCAGAGCCA GAAACATCTT 
GCACCTCCGC CCCTTCTTTC TCCCCTTTTA CCTTCTATCA AGCCAACCGT TCGTAAGACC 
AGGCAAGATG GGAGTCAGGA AGCTCCAGAG GCTCCCTTGT CCTCAGAGCT GGAGCCTTTC 
CACCCAAAGC CTAAAATTAG AACTCGGAAG TCCTCCAGAA TGACACCCTT TCCAGCTACC 
TCTGCTGCCC CTGAGCCCCA CCCTTCCACC TCCACAGCCC AGCCAGTCAC TCCCAAGCCC 
ACATCTCAGG CCACTAGGAG CAGGACAAAT AGGTCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC AGGTTACTAG GGGAAGAAAA AGTAGATCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCA CAGCCCTTGA GCTCCAGCCT TCCACCTCCA CCGACCGACC TGTCACCTCT 
GAACCCACCT CTCAGGCTAC TAGGGGAAGA AAAAATAGAT CCTCTGTCAA GACCCCTGAA 
CCAGTTGTCC CCACAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCCTGTCACT 
TCTGAGCCCA CATATCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TGCCCACAGC CCCTGAGCTC CGGCCTTCCA CCTCCACAGA CCGACCTGTC 
ACCCCCAAGC CCACATCTCG GACCACTAGG AGCAGGACAA ATATGTCCTC TGTCAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGATTT CCACCTCCAC AGACCAACCT 
GTCACCCCTA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTGAAG 
AACCCTGAAT CAACTGTCCC TATAGCCCCT GAGCTCCCAC CTTCCACCTC CACAGAGCAG 
CCTGTCACCC CTGAGCCCAC ATCTCGGGCT ACTAGGGGAA GAAAAAATAG ATCCTCTGGC 
AAGACCCCTG AAACACTTGT CCCCACAGCC CCTAAGCTCG AGCCTTCCAC TTCCACAGAC 
CAACCTGTCA CTCCTGAGCC CACATCTCAG GCCACCAGGG GCAGGACAAA TAGGTCCTCT 
GTGAAGACCC CTGAAACAGT TGTCCCCACA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAGCCTG TTACCCCTGA GCCTACGTCT CAGGCTACTA GGGGAAGAAC AGATAGATCC 
TCTGTCAAGA CTCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTACAGGC TTCCGCCTCC 
ACAGACCAGC CTGTCACCTC TGAGCCCACA TCTCGGACCA CTAGGGGAAG AAAAAATCGG 
TCCTCTGTCA AGACCCCTGA AACAGTTGTG CCCGCAGCCC CTGAGCTCCA GCCTTCCACC 
TCCACAGACC AACCTGTCAC CCCTGAGCCC ACATCTCGGG CCACTAGGGG CAGGACAAAT 
AGGTCCTCTG TCAAGACCCC TGAATCAATT GTCCCTATAG CCCCTGAGCT TCAGCCTTCC 
ACCTCCAGAA ACCAGCTTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GTGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAGCCA GTTGTCCCCA CAGCCCCTGA GCCCCATCCT 
ACCACCTCCA CAGACCAGCC TGTCACCCCC AAGCTCACAT CTAGGGCCAC TAGGAGAAAG 
ACAAATAGGT CCTCTGTCAA GACTCCCAAA CCAGTTGAAC CAGCAGCCTC TGATCTTGAG 
CCTTTTACCC CCACAGACCA GTCCGTCACC CCTGAGGCCA TAGCTCAGGG TGGTCAGAGC 
AAAACACTGA GGTCTTCCAC AGTAAGAGCT ATGCCGGTTC CTACCACCCC TGAATTCCAA 
TCTCCTGTCA CCACAGACCA GCCTATTTCC CCTGAGCCTA TTACTCAACC CAGTTGCATC 
AAGAGGCAGA GAGCCGCTGG GAACCCTGGC TCCCTCGCAG CTCCCATTGA CCATAAGCCT 
TGCTCTGCAC CCTTGGAACC TAAATCCCAG GCCTCAAGGA ACCAAAGATG GGGAGCAGTG 
AGAGCAGCTG AATCCCTTAC AGCCATTCCT GAGCCTGCCT CTCCCCAGCT TCTTGAGACA 
CCAATTCATG CCTCCCAGAT CCAAAAGGTG GAACCAGCAG GTAGATCTAG GTTCACCCCG 
GAGCTCCAGC CTAAGGCCTC TCAAAGCCGC AAGAGGTCTT TAGCTACCAT GGATTCACCA 
CCACATCAAA AACAGCCCCA AAGAGGGGAA GTCTCCCAGA AGACAGTGAT TATCAAGGAA 
GAGGAAGAAG ATACTGCAGA GAAGCCAGGG AAGGAAGAGG ATGTCGTGAC TCCAAAACCA 
GGCAAGAGAA AGAGAGACCA GGCAGAGGAG GAGCCCAACA GAATACCAAG CCGCAGCCTC 
CGACGGACCA AACTTAACCA AGAATCAACA GCCCCCAAAG TGCTCTTCAC AGGAGTGGTG 
GATGCTCGGG GAGAGCGGGC TGTGCTGGCA CTGGGGGGAA GTCTGGCTGG TTCAGCGGCA 
GAGGCTTCCC ACCTGGTCAC TGATCGCATC CGCCGGACAG TCAAGTTCCT GTGTGCCCTG 
GGGCGGGGAA TCCCCATTCT GTCCCTGGAC TGGCTGCATC AGTCCCGCAA GGCTGGTTTC 
TTCTTACCCC CGGATGAATA TGTGGTGACC GACCCTGAGC AAGAGAAGAA CTTTGGCTTT 
AGCCTTCAAG ACGCACTGAG CAGGGCTCGG GAGCGAAGGC TGCTAGAGGG CTATGAGATC 
TATGTGACCC CTGGAGTCCA GCCACCACCA CCTCAGATGG GAGAGATTAT TAGCTGCTGT 
GGAGGCACAT ACCTACCCAG CATGCCTCGG TCCTATAAGC CTCAGAGAGT TGTGATCACA 
TGCCCTCAGG ACTTCCCTCA TTGCTCCATT CCACTACGGG TTGGGCTGCC CCTCCTCTCG 
CCTGAGTTCC TGCTGACTGG AGTGCTGAAG CAGGAAGCCA AGCCAGAGGC CTTTGTCCTC 
TCCCCTTTGG AGATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011520.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867009.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OHu55010
Clone ID Related Accession (Same CDS sequence)	XM_017011520.1 , XM_017011520.2
Accession Version	XM_017011520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGTC CAGAGCATGG AGGATGAACC TACCCAGGCC 
TTCATGTTGA CTCCACCCCA AGAGCTTGGC CCTTCCCATT GCAGCTTCCA GACAACAGGT 
ACCCTAGATG AACCATGGGA GGTCCTGGCT ACACAGCCAT TCTGTCTGAG AGAGTCTGAG 
GACTCTGAGA CCCAGCCTTT TGACACGCAC CTTGAGGCCT ATGGACCTTG CCTGTCTCCA 
CCTAGGGCAA TACCAGGAGA CCAACATCCA GAGAGCCCAG TTCACACAGA GCCAATGGGG 
ATTCAAGGCA GAGGGAGGCA GACTGTGGAT AAAGTCATGG GTATACCAAA AGAAACAGCA 
GAGAGGGTGG GCCCTGAGAG AGGGCCATTG GAGAGAGAAA CTGAGAAACT GCTACCAGAA 
AGACAGACAG ATGTGACAGG AGAGGAAGAA TTAACCAAGG GGAAACAGGA CAGAGAACAA 
AAACAGTTGT TAGCTAGAGA CACCCAGAGA CAAGAATCTG ACAAAAATGG GGAAAGTGCA 
AGTCCTGAAA GAGATAGGGA GAGTTTGAAG GTAGAAATTG AGACATCTGA GGAAATACAA 
GAGAAACAAG TACAGAAGCA GACCCTTCCA AGCAAAGCAT TTGAGAGAGA AGTAGAGAGA 
CCAGTAGCAA ACAGAGAGTG CGATCCAGCC GAGTTAGAAG AGAAGGTGCC CAAAGTGATC 
CTGGAGAGAG ATACACAGAG AGGGGAGCCA GAGGGAGGGA GCCAGGACCA GAAAGGGCAG 
GCCTCCAGCC CAACACCAGA GCCTGGGGTG GGGGCGGGGG ACCTTCCGGG ACCTACCTCA 
GCCCCCGTAC CTTCTGGGAG CCAGTCAGGT GGAAGGGGAT CCCCAGTGAG CCCCAGGAGG 
CATCAGAAAG GCCTCCTGAA TTGCAAGATG CCACCTGCTG AGAAGGCTTC CAGGATCAGA 
GCTGCTGAGA AGGTTTCCAG GGGCGATCAG GAATCTCCAG ATGCTTGTCT GCCTCCTACA 
GTACCTGAAG CCCCAGCCCC ACCCCAAAAG CCCCTTAACT CTCAGAGCCA GAAACATCTT 
GCACCTCCGC CCCTTCTTTC TCCCCTTTTA CCTTCTATCA AGCCAACCGT TCGTAAGACC 
AGGCAAGATG GGAGTCAGGA AGCTCCAGAG GCTCCCTTGT CCTCAGAGCT GGAGCCTTTC 
CACCCAAAGC CTAAAATTAG AACTCGGAAG TCCTCCAGAA TGACACCCTT TCCAGCTACC 
TCTGCTGCCC CTGAGCCCCA CCCTTCCACC TCCACAGCCC AGCCAGTCAC TCCCAAGCCC 
ACATCTCAGG CCACTAGGAG CAGGACAAAT AGGTCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC AGGTTACTAG GGGAAGAAAA AGTAGATCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCA CAGCCCTTGA GCTCCAGCCT TCCACCTCCA CCGACCGACC TGTCACCTCT 
GAACCCACCT CTCAGGCTAC TAGGGGAAGA AAAAATAGAT CCTCTGTCAA GACCCCTGAA 
CCAGTTGTCC CCACAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCCTGTCACT 
TCTGAGCCCA CATATCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TGCCCACAGC CCCTGAGCTC CGGCCTTCCA CCTCCACAGA CCGACCTGTC 
ACCCCCAAGC CCACATCTCG GACCACTAGG AGCAGGACAA ATATGTCCTC TGTCAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGATTT CCACCTCCAC AGACCAACCT 
GTCACCCCTA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTGAAG 
AACCCTGAAT CAACTGTCCC TATAGCCCCT GAGCTCCCAC CTTCCACCTC CACAGAGCAG 
CCTGTCACCC CTGAGCCCAC ATCTCGGGCT ACTAGGGGAA GAAAAAATAG ATCCTCTGGC 
AAGACCCCTG AAACACTTGT CCCCACAGCC CCTAAGCTCG AGCCTTCCAC TTCCACAGAC 
CAACCTGTCA CTCCTGAGCC CACATCTCAG GCCACCAGGG GCAGGACAAA TAGGTCCTCT 
GTGAAGACCC CTGAAACAGT TGTCCCCACA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAGCCTG TTACCCCTGA GCCTACGTCT CAGGCTACTA GGGGAAGAAC AGATAGATCC 
TCTGTCAAGA CTCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTACAGGC TTCCGCCTCC 
ACAGACCAGC CTGTCACCTC TGAGCCCACA TCTCGGACCA CTAGGGGAAG AAAAAATCGG 
TCCTCTGTCA AGACCCCTGA AACAGTTGTG CCCGCAGCCC CTGAGCTCCA GCCTTCCACC 
TCCACAGACC AACCTGTCAC CCCTGAGCCC ACATCTCGGG CCACTAGGGG CAGGACAAAT 
AGGTCCTCTG TCAAGACCCC TGAATCAATT GTCCCTATAG CCCCTGAGCT TCAGCCTTCC 
ACCTCCAGAA ACCAGCTTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GTGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAGCCA GTTGTCCCCA CAGCCCCTGA GCCCCATCCT 
ACCACCTCCA CAGACCAGCC TGTCACCCCC AAGCTCACAT CTAGGGCCAC TAGGAGAAAG 
ACAAATAGGT CCTCTGTCAA GACTCCCAAA CCAGTTGAAC CAGCAGCCTC TGATCTTGAG 
CCTTTTACCC CCACAGACCA GTCCGTCACC CCTGAGGCCA TAGCTCAGGG TGGTCAGAGC 
AAAACACTGA GGTCTTCCAC AGTAAGAGCT ATGCCGGTTC CTACCACCCC TGAATTCCAA 
TCTCCTGTCA CCACAGACCA GCCTATTTCC CCTGAGCCTA TTACTCAACC CAGTTGCATC 
AAGAGGCAGA GAGCCGCTGG GAACCCTGGC TCCCTCGCAG CTCCCATTGA CCATAAGCCT 
TGCTCTGCAC CCTTGGAACC TAAATCCCAG GCCTCAAGGA ACCAAAGATG GGGAGCAGTG 
AGAGCAGCTG AATCCCTTAC AGCCATTCCT GAGCCTGCCT CTCCCCAGCT TCTTGAGACA 
CCAATTCATG CCTCCCAGAT CCAAAAGGTG GAACCAGCAG GTAGATCTAG GTTCACCCCG 
GAGCTCCAGC CTAAGGCCTC TCAAAGCCGC AAGAGGTCTT TAGCTACCAT GGATTCACCA 
CCACATCAAA AACAGCCCCA AAGAGGGGAA GTCTCCCAGA AGACAGTGAT TATCAAGGAA 
GAGGAAGAAG ATACTGCAGA GAAGCCAGGG AAGGAAGAGG ATGTCGTGAC TCCAAAACCA 
GGCAAGAGAA AGAGAGACCA GGCAGAGGAG GAGCCCAACA GAATACCAAG CCGCAGCCTC 
CGACGGACCA AACTTAACCA AGAATCAACA GCCCCCAAAG TGCTCTTCAC AGGAGTGGTG 
GATGCTCGGG GAGAGCGGGC TGTGCTGGCA CTGGGGGGAA GTCTGGCTGG TTCAGCGGCA 
GAGGCTTCCC ACCTGGTCAC TGATCGCATC CGCCGGACAG TCAAGTTCCT GTGTGCCCTG 
GGGCGGGGAA TCCCCATTCT GTCCCTGGAC TGGCTGCATC AGTCCCGCAA GGCTGGTTTC 
TTCTTACCCC CGGATGAATA TGTGGTGACC GACCCTGAGC AAGAGAAGAA CTTTGGCTTT 
AGCCTTCAAG ACGCACTGAG CAGGGCTCGG GAGCGAAGGC TGCTAGAGGG CTATGAGATC 
TATGTGACCC CTGGAGTCCA GCCACCACCA CCTCAGATGG GAGAGATTAT TAGCTGCTGT 
GGAGGCACAT ACCTACCCAG CATGCCTCGG TCCTATAAGC CTCAGAGAGT TGTGATCACA 
TGCCCTCAGG ACTTCCCTCA TTGCTCCATT CCACTACGGG TTGGGCTGCC CCTCCTCTCG 
CCTGAGTTCC TGCTGACTGG AGTGCTGAAG CAGGAAGCCA AGCCAGAGGC CTTTGTCCTC 
TCCCCTTTGG AGATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867009.1
CDS	43..6309
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011520.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011520.2

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGTC CAGAGCATGG AGGATGAACC TACCCAGGCC 
TTCATGTTGA CTCCACCCCA AGAGCTTGGC CCTTCCCATT GCAGCTTCCA GACAACAGGT 
ACCCTAGATG AACCATGGGA GGTCCTGGCT ACACAGCCAT TCTGTCTGAG AGAGTCTGAG 
GACTCTGAGA CCCAGCCTTT TGACACGCAC CTTGAGGCCT ATGGACCTTG CCTGTCTCCA 
CCTAGGGCAA TACCAGGAGA CCAACATCCA GAGAGCCCAG TTCACACAGA GCCAATGGGG 
ATTCAAGGCA GAGGGAGGCA GACTGTGGAT AAAGTCATGG GTATACCAAA AGAAACAGCA 
GAGAGGGTGG GCCCTGAGAG AGGGCCATTG GAGAGAGAAA CTGAGAAACT GCTACCAGAA 
AGACAGACAG ATGTGACAGG AGAGGAAGAA TTAACCAAGG GGAAACAGGA CAGAGAACAA 
AAACAGTTGT TAGCTAGAGA CACCCAGAGA CAAGAATCTG ACAAAAATGG GGAAAGTGCA 
AGTCCTGAAA GAGATAGGGA GAGTTTGAAG GTAGAAATTG AGACATCTGA GGAAATACAA 
GAGAAACAAG TACAGAAGCA GACCCTTCCA AGCAAAGCAT TTGAGAGAGA AGTAGAGAGA 
CCAGTAGCAA ACAGAGAGTG CGATCCAGCC GAGTTAGAAG AGAAGGTGCC CAAAGTGATC 
CTGGAGAGAG ATACACAGAG AGGGGAGCCA GAGGGAGGGA GCCAGGACCA GAAAGGGCAG 
GCCTCCAGCC CAACACCAGA GCCTGGGGTG GGGGCGGGGG ACCTTCCGGG ACCTACCTCA 
GCCCCCGTAC CTTCTGGGAG CCAGTCAGGT GGAAGGGGAT CCCCAGTGAG CCCCAGGAGG 
CATCAGAAAG GCCTCCTGAA TTGCAAGATG CCACCTGCTG AGAAGGCTTC CAGGATCAGA 
GCTGCTGAGA AGGTTTCCAG GGGCGATCAG GAATCTCCAG ATGCTTGTCT GCCTCCTACA 
GTACCTGAAG CCCCAGCCCC ACCCCAAAAG CCCCTTAACT CTCAGAGCCA GAAACATCTT 
GCACCTCCGC CCCTTCTTTC TCCCCTTTTA CCTTCTATCA AGCCAACCGT TCGTAAGACC 
AGGCAAGATG GGAGTCAGGA AGCTCCAGAG GCTCCCTTGT CCTCAGAGCT GGAGCCTTTC 
CACCCAAAGC CTAAAATTAG AACTCGGAAG TCCTCCAGAA TGACACCCTT TCCAGCTACC 
TCTGCTGCCC CTGAGCCCCA CCCTTCCACC TCCACAGCCC AGCCAGTCAC TCCCAAGCCC 
ACATCTCAGG CCACTAGGAG CAGGACAAAT AGGTCCTCTG TCAAGACCCC TGAACCAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT CACCTCTGAG 
CCCACATCTC AGGTTACTAG GGGAAGAAAA AGTAGATCCT CTGTCAAGAC CCCTGAAACA 
GTTGTGCCCA CAGCCCTTGA GCTCCAGCCT TCCACCTCCA CCGACCGACC TGTCACCTCT 
GAACCCACCT CTCAGGCTAC TAGGGGAAGA AAAAATAGAT CCTCTGTCAA GACCCCTGAA 
CCAGTTGTCC CCACAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA GCCTGTCACT 
TCTGAGCCCA CATATCAGGC TACTAGGGGA AGAAAAAATA GATCCTCTGT CAAGACCCCT 
GAACCAGTTG TGCCCACAGC CCCTGAGCTC CGGCCTTCCA CCTCCACAGA CCGACCTGTC 
ACCCCCAAGC CCACATCTCG GACCACTAGG AGCAGGACAA ATATGTCCTC TGTCAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGATTT CCACCTCCAC AGACCAACCT 
GTCACCCCTA AGCCCACATC TCGGACCACT AGGAGCAGGA CAAATATGTC CTCTGTGAAG 
AACCCTGAAT CAACTGTCCC TATAGCCCCT GAGCTCCCAC CTTCCACCTC CACAGAGCAG 
CCTGTCACCC CTGAGCCCAC ATCTCGGGCT ACTAGGGGAA GAAAAAATAG ATCCTCTGGC 
AAGACCCCTG AAACACTTGT CCCCACAGCC CCTAAGCTCG AGCCTTCCAC TTCCACAGAC 
CAACCTGTCA CTCCTGAGCC CACATCTCAG GCCACCAGGG GCAGGACAAA TAGGTCCTCT 
GTGAAGACCC CTGAAACAGT TGTCCCCACA GCCCCTGAGC TCCAGCCTTC CACCTCCACA 
GACCAGCCTG TTACCCCTGA GCCTACGTCT CAGGCTACTA GGGGAAGAAC AGATAGATCC 
TCTGTCAAGA CTCCTGAAAC AGTTGTCCCC ACAGCCCCTG AGCTACAGGC TTCCGCCTCC 
ACAGACCAGC CTGTCACCTC TGAGCCCACA TCTCGGACCA CTAGGGGAAG AAAAAATCGG 
TCCTCTGTCA AGACCCCTGA AACAGTTGTG CCCGCAGCCC CTGAGCTCCA GCCTTCCACC 
TCCACAGACC AACCTGTCAC CCCTGAGCCC ACATCTCGGG CCACTAGGGG CAGGACAAAT 
AGGTCCTCTG TCAAGACCCC TGAATCAATT GTCCCTATAG CCCCTGAGCT TCAGCCTTCC 
ACCTCCAGAA ACCAGCTTGT CACCCCTGAG CCCACATCTC GGGCCACTAG GTGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAGCCA GTTGTCCCCA CAGCCCCTGA GCCCCATCCT 
ACCACCTCCA CAGACCAGCC TGTCACCCCC AAGCTCACAT CTAGGGCCAC TAGGAGAAAG 
ACAAATAGGT CCTCTGTCAA GACTCCCAAA CCAGTTGAAC CAGCAGCCTC TGATCTTGAG 
CCTTTTACCC CCACAGACCA GTCCGTCACC CCTGAGGCCA TAGCTCAGGG TGGTCAGAGC 
AAAACACTGA GGTCTTCCAC AGTAAGAGCT ATGCCGGTTC CTACCACCCC TGAATTCCAA 
TCTCCTGTCA CCACAGACCA GCCTATTTCC CCTGAGCCTA TTACTCAACC CAGTTGCATC 
AAGAGGCAGA GAGCCGCTGG GAACCCTGGC TCCCTCGCAG CTCCCATTGA CCATAAGCCT 
TGCTCTGCAC CCTTGGAACC TAAATCCCAG GCCTCAAGGA ACCAAAGATG GGGAGCAGTG 
AGAGCAGCTG AATCCCTTAC AGCCATTCCT GAGCCTGCCT CTCCCCAGCT TCTTGAGACA 
CCAATTCATG CCTCCCAGAT CCAAAAGGTG GAACCAGCAG GTAGATCTAG GTTCACCCCG 
GAGCTCCAGC CTAAGGCCTC TCAAAGCCGC AAGAGGTCTT TAGCTACCAT GGATTCACCA 
CCACATCAAA AACAGCCCCA AAGAGGGGAA GTCTCCCAGA AGACAGTGAT TATCAAGGAA 
GAGGAAGAAG ATACTGCAGA GAAGCCAGGG AAGGAAGAGG ATGTCGTGAC TCCAAAACCA 
GGCAAGAGAA AGAGAGACCA GGCAGAGGAG GAGCCCAACA GAATACCAAG CCGCAGCCTC 
CGACGGACCA AACTTAACCA AGAATCAACA GCCCCCAAAG TGCTCTTCAC AGGAGTGGTG 
GATGCTCGGG GAGAGCGGGC TGTGCTGGCA CTGGGGGGAA GTCTGGCTGG TTCAGCGGCA 
GAGGCTTCCC ACCTGGTCAC TGATCGCATC CGCCGGACAG TCAAGTTCCT GTGTGCCCTG 
GGGCGGGGAA TCCCCATTCT GTCCCTGGAC TGGCTGCATC AGTCCCGCAA GGCTGGTTTC 
TTCTTACCCC CGGATGAATA TGTGGTGACC GACCCTGAGC AAGAGAAGAA CTTTGGCTTT 
AGCCTTCAAG ACGCACTGAG CAGGGCTCGG GAGCGAAGGC TGCTAGAGGG CTATGAGATC 
TATGTGACCC CTGGAGTCCA GCCACCACCA CCTCAGATGG GAGAGATTAT TAGCTGCTGT 
GGAGGCACAT ACCTACCCAG CATGCCTCGG TCCTATAAGC CTCAGAGAGT TGTGATCACA 
TGCCCTCAGG ACTTCCCTCA TTGCTCCATT CCACTACGGG TTGGGCTGCC CCTCCTCTCG 
CCTGAGTTCC TGCTGACTGG AGTGCTGAAG CAGGAAGCCA AGCCAGAGGC CTTTGTCCTC 
TCCCCTTTGG AGATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011521.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867010.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

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CloneID	OHu55011
Clone ID Related Accession (Same CDS sequence)	XM_017011521.2 , XM_017011521.1
Accession Version	XM_017011521.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGCCTCCT GAATTGCAAG 
ATGCCACCTG CTGAGAAGGC TTCCAGGATC AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT 
CAGGAATCTC CAGATGCTTG TCTGCCTCCT ACAGTACCTG AAGCCCCAGC CCCACCCCAA 
AAGCCCCTTA ACTCTCAGAG CCAGAAACAT CTTGCACCTC CGCCCCTTCT TTCTCCCCTT 
TTACCTTCTA TCAAGCCAAC CGTTCGTAAG ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA 
GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT TTCCACCCAA AGCCTAAAAT TAGAACTCGG 
AAGTCCTCCA GAATGACACC CTTTCCAGCT ACCTCTGCTG CCCCTGAGCC CCACCCTTCC 
ACCTCCACAG CCCAGCCAGT CACTCCCAAG CCCACATCTC AGGCCACTAG GAGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAACCA GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCC TGTCACCTCT GAGCCCACAT CTCAGGTTAC TAGGGGAAGA 
AAAAGTAGAT CCTCTGTCAA GACCCCTGAA ACAGTTGTGC CCACAGCCCT TGAGCTCCAG 
CCTTCCACCT CCACCGACCG ACCTGTCACC TCTGAACCCA CCTCTCAGGC TACTAGGGGA 
AGAAAAAATA GATCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACTTCTGAGC CCACATATCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTGCCCAC AGCCCCTGAG 
CTCCGGCCTT CCACCTCCAC AGACCGACCT GTCACCCCCA AGCCCACATC TCGGACCACT 
AGGAGCAGGA CAAATATGTC CTCTGTCAAG ACCCCTGAAA CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGA TTTCCACCTC CACAGACCAA CCTGTCACCC CTAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTG AAGAACCCTG AATCAACTGT CCCTATAGCC 
CCTGAGCTCC CACCTTCCAC CTCCACAGAG CAGCCTGTCA CCCCTGAGCC CACATCTCGG 
GCTACTAGGG GAAGAAAAAA TAGATCCTCT GGCAAGACCC CTGAAACACT TGTCCCCACA 
GCCCCTAAGC TCGAGCCTTC CACTTCCACA GACCAACCTG TCACTCCTGA GCCCACATCT 
CAGGCCACCA GGGGCAGGAC AAATAGGTCC TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTTACCCC TGAGCCTACG 
TCTCAGGCTA CTAGGGGAAG AACAGATAGA TCCTCTGTCA AGACTCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTACA GGCTTCCGCC TCCACAGACC AGCCTGTCAC CTCTGAGCCC 
ACATCTCGGA CCACTAGGGG AAGAAAAAAT CGGTCCTCTG TCAAGACCCC TGAAACAGTT 
GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAACCTGT CACCCCTGAG 
CCCACATCTC GGGCCACTAG GGGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAATCA 
ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT TCCACCTCCA GAAACCAGCT TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGTGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAG 
CCAGTTGTCC CCACAGCCCC TGAGCCCCAT CCTACCACCT CCACAGACCA GCCTGTCACC 
CCCAAGCTCA CATCTAGGGC CACTAGGAGA AAGACAAATA GGTCCTCTGT CAAGACTCCC 
AAACCAGTTG AACCAGCAGC CTCTGATCTT GAGCCTTTTA CCCCCACAGA CCAGTCCGTC 
ACCCCTGAGG CCATAGCTCA GGGTGGTCAG AGCAAAACAC TGAGGTCTTC CACAGTAAGA 
GCTATGCCGG TTCCTACCAC CCCTGAATTC CAATCTCCTG TCACCACAGA CCAGCCTATT 
TCCCCTGAGC CTATTACTCA ACCCAGTTGC ATCAAGAGGC AGAGAGCCGC TGGGAACCCT 
GGCTCCCTCG CAGCTCCCAT TGACCATAAG CCTTGCTCTG CACCCTTGGA ACCTAAATCC 
CAGGCCTCAA GGAACCAAAG ATGGGGAGCA GTGAGAGCAG CTGAATCCCT TACAGCCATT 
CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG ACACCAATTC ATGCCTCCCA GATCCAAAAG 
GTGGAACCAG CAGGTAGATC TAGGTTCACC CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC 
CGCAAGAGGT CTTTAGCTAC CATGGATTCA CCACCACATC AAAAACAGCC CCAAAGAGGG 
GAAGTCTCCC AGAAGACAGT GATTATCAAG GAAGAGGAAG AAGATACTGC AGAGAAGCCA 
GGGAAGGAAG AGGATGTCGT GACTCCAAAA CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG 
GAGGAGCCCA ACAGAATACC AAGCCGCAGC CTCCGACGGA CCAAACTTAA CCAAGAATCA 
ACAGCCCCCA AAGTGCTCTT CACAGGAGTG GTGGATGCTC GGGGAGAGCG GGCTGTGCTG 
GCACTGGGGG GAAGTCTGGC TGGTTCAGCG GCAGAGGCTT CCCACCTGGT CACTGATCGC 
ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC CTGGGGCGGG GAATCCCCAT TCTGTCCCTG 
GACTGGCTGC ATCAGTCCCG CAAGGCTGGT TTCTTCTTAC CCCCGGATGA ATATGTGGTG 
ACCGACCCTG AGCAAGAGAA GAACTTTGGC TTTAGCCTTC AAGACGCACT GAGCAGGGCT 
CGGGAGCGAA GGCTGCTAGA GGGCTATGAG ATCTATGTGA CCCCTGGAGT CCAGCCACCA 
CCACCTCAGA TGGGAGAGAT TATTAGCTGC TGTGGAGGCA CATACCTACC CAGCATGCCT 
CGGTCCTATA AGCCTCAGAG AGTTGTGATC ACATGCCCTC AGGACTTCCC TCATTGCTCC 
ATTCCACTAC GGGTTGGGCT GCCCCTCCTC TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG 
AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867010.1
CDS	43..5742
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011521.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011521.2

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGCCTCCT GAATTGCAAG 
ATGCCACCTG CTGAGAAGGC TTCCAGGATC AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT 
CAGGAATCTC CAGATGCTTG TCTGCCTCCT ACAGTACCTG AAGCCCCAGC CCCACCCCAA 
AAGCCCCTTA ACTCTCAGAG CCAGAAACAT CTTGCACCTC CGCCCCTTCT TTCTCCCCTT 
TTACCTTCTA TCAAGCCAAC CGTTCGTAAG ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA 
GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT TTCCACCCAA AGCCTAAAAT TAGAACTCGG 
AAGTCCTCCA GAATGACACC CTTTCCAGCT ACCTCTGCTG CCCCTGAGCC CCACCCTTCC 
ACCTCCACAG CCCAGCCAGT CACTCCCAAG CCCACATCTC AGGCCACTAG GAGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAACCA GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCC TGTCACCTCT GAGCCCACAT CTCAGGTTAC TAGGGGAAGA 
AAAAGTAGAT CCTCTGTCAA GACCCCTGAA ACAGTTGTGC CCACAGCCCT TGAGCTCCAG 
CCTTCCACCT CCACCGACCG ACCTGTCACC TCTGAACCCA CCTCTCAGGC TACTAGGGGA 
AGAAAAAATA GATCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACTTCTGAGC CCACATATCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTGCCCAC AGCCCCTGAG 
CTCCGGCCTT CCACCTCCAC AGACCGACCT GTCACCCCCA AGCCCACATC TCGGACCACT 
AGGAGCAGGA CAAATATGTC CTCTGTCAAG ACCCCTGAAA CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGA TTTCCACCTC CACAGACCAA CCTGTCACCC CTAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTG AAGAACCCTG AATCAACTGT CCCTATAGCC 
CCTGAGCTCC CACCTTCCAC CTCCACAGAG CAGCCTGTCA CCCCTGAGCC CACATCTCGG 
GCTACTAGGG GAAGAAAAAA TAGATCCTCT GGCAAGACCC CTGAAACACT TGTCCCCACA 
GCCCCTAAGC TCGAGCCTTC CACTTCCACA GACCAACCTG TCACTCCTGA GCCCACATCT 
CAGGCCACCA GGGGCAGGAC AAATAGGTCC TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTTACCCC TGAGCCTACG 
TCTCAGGCTA CTAGGGGAAG AACAGATAGA TCCTCTGTCA AGACTCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTACA GGCTTCCGCC TCCACAGACC AGCCTGTCAC CTCTGAGCCC 
ACATCTCGGA CCACTAGGGG AAGAAAAAAT CGGTCCTCTG TCAAGACCCC TGAAACAGTT 
GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAACCTGT CACCCCTGAG 
CCCACATCTC GGGCCACTAG GGGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAATCA 
ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT TCCACCTCCA GAAACCAGCT TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGTGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAG 
CCAGTTGTCC CCACAGCCCC TGAGCCCCAT CCTACCACCT CCACAGACCA GCCTGTCACC 
CCCAAGCTCA CATCTAGGGC CACTAGGAGA AAGACAAATA GGTCCTCTGT CAAGACTCCC 
AAACCAGTTG AACCAGCAGC CTCTGATCTT GAGCCTTTTA CCCCCACAGA CCAGTCCGTC 
ACCCCTGAGG CCATAGCTCA GGGTGGTCAG AGCAAAACAC TGAGGTCTTC CACAGTAAGA 
GCTATGCCGG TTCCTACCAC CCCTGAATTC CAATCTCCTG TCACCACAGA CCAGCCTATT 
TCCCCTGAGC CTATTACTCA ACCCAGTTGC ATCAAGAGGC AGAGAGCCGC TGGGAACCCT 
GGCTCCCTCG CAGCTCCCAT TGACCATAAG CCTTGCTCTG CACCCTTGGA ACCTAAATCC 
CAGGCCTCAA GGAACCAAAG ATGGGGAGCA GTGAGAGCAG CTGAATCCCT TACAGCCATT 
CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG ACACCAATTC ATGCCTCCCA GATCCAAAAG 
GTGGAACCAG CAGGTAGATC TAGGTTCACC CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC 
CGCAAGAGGT CTTTAGCTAC CATGGATTCA CCACCACATC AAAAACAGCC CCAAAGAGGG 
GAAGTCTCCC AGAAGACAGT GATTATCAAG GAAGAGGAAG AAGATACTGC AGAGAAGCCA 
GGGAAGGAAG AGGATGTCGT GACTCCAAAA CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG 
GAGGAGCCCA ACAGAATACC AAGCCGCAGC CTCCGACGGA CCAAACTTAA CCAAGAATCA 
ACAGCCCCCA AAGTGCTCTT CACAGGAGTG GTGGATGCTC GGGGAGAGCG GGCTGTGCTG 
GCACTGGGGG GAAGTCTGGC TGGTTCAGCG GCAGAGGCTT CCCACCTGGT CACTGATCGC 
ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC CTGGGGCGGG GAATCCCCAT TCTGTCCCTG 
GACTGGCTGC ATCAGTCCCG CAAGGCTGGT TTCTTCTTAC CCCCGGATGA ATATGTGGTG 
ACCGACCCTG AGCAAGAGAA GAACTTTGGC TTTAGCCTTC AAGACGCACT GAGCAGGGCT 
CGGGAGCGAA GGCTGCTAGA GGGCTATGAG ATCTATGTGA CCCCTGGAGT CCAGCCACCA 
CCACCTCAGA TGGGAGAGAT TATTAGCTGC TGTGGAGGCA CATACCTACC CAGCATGCCT 
CGGTCCTATA AGCCTCAGAG AGTTGTGATC ACATGCCCTC AGGACTTCCC TCATTGCTCC 
ATTCCACTAC GGGTTGGGCT GCCCCTCCTC TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG 
AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011521.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867010.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

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CloneID	OHu55011
Clone ID Related Accession (Same CDS sequence)	XM_017011521.2 , XM_017011521.1
Accession Version	XM_017011521.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGCCTCCT GAATTGCAAG 
ATGCCACCTG CTGAGAAGGC TTCCAGGATC AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT 
CAGGAATCTC CAGATGCTTG TCTGCCTCCT ACAGTACCTG AAGCCCCAGC CCCACCCCAA 
AAGCCCCTTA ACTCTCAGAG CCAGAAACAT CTTGCACCTC CGCCCCTTCT TTCTCCCCTT 
TTACCTTCTA TCAAGCCAAC CGTTCGTAAG ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA 
GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT TTCCACCCAA AGCCTAAAAT TAGAACTCGG 
AAGTCCTCCA GAATGACACC CTTTCCAGCT ACCTCTGCTG CCCCTGAGCC CCACCCTTCC 
ACCTCCACAG CCCAGCCAGT CACTCCCAAG CCCACATCTC AGGCCACTAG GAGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAACCA GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCC TGTCACCTCT GAGCCCACAT CTCAGGTTAC TAGGGGAAGA 
AAAAGTAGAT CCTCTGTCAA GACCCCTGAA ACAGTTGTGC CCACAGCCCT TGAGCTCCAG 
CCTTCCACCT CCACCGACCG ACCTGTCACC TCTGAACCCA CCTCTCAGGC TACTAGGGGA 
AGAAAAAATA GATCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACTTCTGAGC CCACATATCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTGCCCAC AGCCCCTGAG 
CTCCGGCCTT CCACCTCCAC AGACCGACCT GTCACCCCCA AGCCCACATC TCGGACCACT 
AGGAGCAGGA CAAATATGTC CTCTGTCAAG ACCCCTGAAA CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGA TTTCCACCTC CACAGACCAA CCTGTCACCC CTAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTG AAGAACCCTG AATCAACTGT CCCTATAGCC 
CCTGAGCTCC CACCTTCCAC CTCCACAGAG CAGCCTGTCA CCCCTGAGCC CACATCTCGG 
GCTACTAGGG GAAGAAAAAA TAGATCCTCT GGCAAGACCC CTGAAACACT TGTCCCCACA 
GCCCCTAAGC TCGAGCCTTC CACTTCCACA GACCAACCTG TCACTCCTGA GCCCACATCT 
CAGGCCACCA GGGGCAGGAC AAATAGGTCC TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTTACCCC TGAGCCTACG 
TCTCAGGCTA CTAGGGGAAG AACAGATAGA TCCTCTGTCA AGACTCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTACA GGCTTCCGCC TCCACAGACC AGCCTGTCAC CTCTGAGCCC 
ACATCTCGGA CCACTAGGGG AAGAAAAAAT CGGTCCTCTG TCAAGACCCC TGAAACAGTT 
GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAACCTGT CACCCCTGAG 
CCCACATCTC GGGCCACTAG GGGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAATCA 
ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT TCCACCTCCA GAAACCAGCT TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGTGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAG 
CCAGTTGTCC CCACAGCCCC TGAGCCCCAT CCTACCACCT CCACAGACCA GCCTGTCACC 
CCCAAGCTCA CATCTAGGGC CACTAGGAGA AAGACAAATA GGTCCTCTGT CAAGACTCCC 
AAACCAGTTG AACCAGCAGC CTCTGATCTT GAGCCTTTTA CCCCCACAGA CCAGTCCGTC 
ACCCCTGAGG CCATAGCTCA GGGTGGTCAG AGCAAAACAC TGAGGTCTTC CACAGTAAGA 
GCTATGCCGG TTCCTACCAC CCCTGAATTC CAATCTCCTG TCACCACAGA CCAGCCTATT 
TCCCCTGAGC CTATTACTCA ACCCAGTTGC ATCAAGAGGC AGAGAGCCGC TGGGAACCCT 
GGCTCCCTCG CAGCTCCCAT TGACCATAAG CCTTGCTCTG CACCCTTGGA ACCTAAATCC 
CAGGCCTCAA GGAACCAAAG ATGGGGAGCA GTGAGAGCAG CTGAATCCCT TACAGCCATT 
CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG ACACCAATTC ATGCCTCCCA GATCCAAAAG 
GTGGAACCAG CAGGTAGATC TAGGTTCACC CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC 
CGCAAGAGGT CTTTAGCTAC CATGGATTCA CCACCACATC AAAAACAGCC CCAAAGAGGG 
GAAGTCTCCC AGAAGACAGT GATTATCAAG GAAGAGGAAG AAGATACTGC AGAGAAGCCA 
GGGAAGGAAG AGGATGTCGT GACTCCAAAA CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG 
GAGGAGCCCA ACAGAATACC AAGCCGCAGC CTCCGACGGA CCAAACTTAA CCAAGAATCA 
ACAGCCCCCA AAGTGCTCTT CACAGGAGTG GTGGATGCTC GGGGAGAGCG GGCTGTGCTG 
GCACTGGGGG GAAGTCTGGC TGGTTCAGCG GCAGAGGCTT CCCACCTGGT CACTGATCGC 
ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC CTGGGGCGGG GAATCCCCAT TCTGTCCCTG 
GACTGGCTGC ATCAGTCCCG CAAGGCTGGT TTCTTCTTAC CCCCGGATGA ATATGTGGTG 
ACCGACCCTG AGCAAGAGAA GAACTTTGGC TTTAGCCTTC AAGACGCACT GAGCAGGGCT 
CGGGAGCGAA GGCTGCTAGA GGGCTATGAG ATCTATGTGA CCCCTGGAGT CCAGCCACCA 
CCACCTCAGA TGGGAGAGAT TATTAGCTGC TGTGGAGGCA CATACCTACC CAGCATGCCT 
CGGTCCTATA AGCCTCAGAG AGTTGTGATC ACATGCCCTC AGGACTTCCC TCATTGCTCC 
ATTCCACTAC GGGTTGGGCT GCCCCTCCTC TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG 
AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867010.1
CDS	43..5742
Translation	MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011521.1

ORF Insert Sequence:

ATGGAGGACA CCCAGGCTAT TGACTGGGAT GTTGAAGAAG AGGAGGAGAC AGAGCAATCC 
AGTGAATCCT TGAGGTGTAA CGTGGAGCCA GTAGGGCGGC TACATATCTT TAGTGGTGCC 
CATGGACCAG AAAAAGATTT CCCACTACAC CTCGGGAAGA ATGTGGTAGG CCGAATGCCT 
GACTGCTCTG TGGCCCTGCC CTTTCCATCT ATCTCCAAAC AACATGCAGA GATTGAAATC 
TTAGCCTGGG ACAAGGCACC TATCCTCCGA GACTGTGGGA GCCTTAATGG TACTCAAATC 
CTGAGACCTC CTAAGGTTTT GAGCCCTGGG GTGAGTCACC GTCTGAGGGA CCAGGAATTG 
ATTCTCTTTG CTGACTTGCT CTGCCAGTAC CATCGCCTGG ATGTCTCTCT GCCCTTTGTC 
TCCCGGGGCC CTCTGACAGT AGAAGAGACA CCCAGAGTAC AGGGAGAAAC TCAACCCCAG 
AGGCTTCTGT TGGCTGAGGA CTCGGAGGAG GAAGTAGATT TTCTTTCTGA AAGGCGTATG 
GTAAAAAAAT CAAGGACCAC ATCTTCCTCT GTGATAGTTC CAGAGAGTGA TGAAGAGGGG 
CATTCCCCGG TCCTGGGCGG CCTTGGGCCG CCTTTTGCCT TCAATTTGAA CAGTGACACA 
GATGTGGAAG AAGGTCAGCA ACCAGCCACA GAGGAGGCCT CCTCAGCTGC CAGAAGAGGT 
GCCACTGTAG AGGCAAAGCA GTCTGAAGCT GAAGTTGTAA CTGAAATCCA GCTTGAAAAG 
GATCAGCCTT TAGTGAAGGA GAGGGACAAT GATACAAAAG TCAAGAGGGG TGCAGGGAAT 
GGGGTGGTTC CAGCTGGGGT GATTCTGGAG AGGAGCCAAC CTCCTGGAGA GGACAGTGAC 
ACAGATGTGG ATGATGACAG CAGGCCTCCT GGAAGGCCAG CTGAGGTCCA TTTGGAAAGG 
GCTCAGCCTT TTGGCTTCAT CGACAGCGAC ACTGATGCGG AAGAAGAGAG GATCCCAGCA 
ACCCCAGTTG TCATTCCTAT GAAGAAGAGG AAGATCTTCC ATGGAGTAGG TACAAGGGGT 
CCTGGAGCAC CAGGCCTGGC CCATCTGCAG GAGAGCCAGG CTGGTAGTGA TACAGATGTG 
GAAGAAGGCA AGGCCCCACA GGCTGTCCCT CTGGAGAAAA GCCAAGCTTC CATGGTTATC 
AACAGCGATA CAGATGACGA GGAAGAAGTC TCAGCAGCGC TGACTTTGGC ACATCTGAAA 
GAGAGCCAGC CTGCTATATG GAACAGAGAT GCAGAAGAGG ACATGCCCCA ACGTGTGGTC 
CTTCTGCAGC GAAGCCAAAC CACCACTGAG AGAGACAGTG ACACAGACGT GGAGGAGGAA 
GAGCTCCCAG TGGAAAATAG AGAAGCTGTC CTCAAGGATC ACACAAAGAT TAGAGCCCTT 
GTTAGAGCAC ATTCAGAAAA GGACCAACCT CCTTTTGGGG ACAGTGATGA CAGTGTGGAA 
GCAGATAAGA GCTCACCTGG GATCCACCTG GAGAGAAGCC AAGCCTCCAC CACAGTGGAC 
ATCAACACAC AAGTGGAGAA GGAAGTCCCG CCAGGGTCAG CCATTATACA TATAAAGAAG 
CATCAGGTGT CTGTGGAGGG GACAAATCAA ACAGATGTGA AAGCAGTTGG GGGACCAGCA 
AAGCTGCTTG TGGTATCTCT AGAGGAAGCC TGGCCTCTGC ATGGGGACTG TGAAACAGAT 
GCAGAGGAGG GCACCTCCCT AACAGCCTCA GTAGTTGCAG ATGTAAGAAA GAGCCAGCTT 
CCAGCAGAAG GGGATGCTGG GGCAGAGTGG GCTGCAGCTG TTCTTAAGCA GGAGAGAGCT 
CATGAGGTGG GGGCCCAGGG TGGGCCACCT GTGGCACAAG TGGAGCAGGA CCTCCCTATC 
TCAAGAGAGA ACCTCACAGA TCTGGTGGTG GACACAGACA CTCTAGGGGA ATCCACCCAG 
CCACAGAGAG AGGGAGCCCA GGTCCCCACA GGAAGGGAGA GAGAACAACA TGTGGGTGGG 
ACCAAGGACT CTGAAGACAA CTATGGTGAT TCTGAAGATC TGGACCTACA AGCTACCCAG 
TGCTTTCTGG AGAATCAGGG CCTGGAAGCA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGTACCCTAG ATGAACCATG GGAGGTCCTG GCTACACAGC CATTCTGTCT GAGAGAGTCT 
GAGGACTCTG AGACCCAGCC TTTTGACACG CACCTTGAGG CCTATGGACC TTGCCTGTCT 
CCACCTAGGG CAATACCAGG AGACCAACAT CCAGAGAGCC CAGTTCACAC AGAGCCAATG 
GGGATTCAAG GCAGAGGGAG GCAGACTGTG GATAAAGTCA TGGGCCTCCT GAATTGCAAG 
ATGCCACCTG CTGAGAAGGC TTCCAGGATC AGAGCTGCTG AGAAGGTTTC CAGGGGCGAT 
CAGGAATCTC CAGATGCTTG TCTGCCTCCT ACAGTACCTG AAGCCCCAGC CCCACCCCAA 
AAGCCCCTTA ACTCTCAGAG CCAGAAACAT CTTGCACCTC CGCCCCTTCT TTCTCCCCTT 
TTACCTTCTA TCAAGCCAAC CGTTCGTAAG ACCAGGCAAG ATGGGAGTCA GGAAGCTCCA 
GAGGCTCCCT TGTCCTCAGA GCTGGAGCCT TTCCACCCAA AGCCTAAAAT TAGAACTCGG 
AAGTCCTCCA GAATGACACC CTTTCCAGCT ACCTCTGCTG CCCCTGAGCC CCACCCTTCC 
ACCTCCACAG CCCAGCCAGT CACTCCCAAG CCCACATCTC AGGCCACTAG GAGCAGGACA 
AATAGGTCCT CTGTCAAGAC CCCTGAACCA GTTGTCCCCA CAGCCCCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCC TGTCACCTCT GAGCCCACAT CTCAGGTTAC TAGGGGAAGA 
AAAAGTAGAT CCTCTGTCAA GACCCCTGAA ACAGTTGTGC CCACAGCCCT TGAGCTCCAG 
CCTTCCACCT CCACCGACCG ACCTGTCACC TCTGAACCCA CCTCTCAGGC TACTAGGGGA 
AGAAAAAATA GATCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACTTCTGAGC CCACATATCA GGCTACTAGG 
GGAAGAAAAA ATAGATCCTC TGTCAAGACC CCTGAACCAG TTGTGCCCAC AGCCCCTGAG 
CTCCGGCCTT CCACCTCCAC AGACCGACCT GTCACCCCCA AGCCCACATC TCGGACCACT 
AGGAGCAGGA CAAATATGTC CTCTGTCAAG ACCCCTGAAA CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGA TTTCCACCTC CACAGACCAA CCTGTCACCC CTAAGCCCAC ATCTCGGACC 
ACTAGGAGCA GGACAAATAT GTCCTCTGTG AAGAACCCTG AATCAACTGT CCCTATAGCC 
CCTGAGCTCC CACCTTCCAC CTCCACAGAG CAGCCTGTCA CCCCTGAGCC CACATCTCGG 
GCTACTAGGG GAAGAAAAAA TAGATCCTCT GGCAAGACCC CTGAAACACT TGTCCCCACA 
GCCCCTAAGC TCGAGCCTTC CACTTCCACA GACCAACCTG TCACTCCTGA GCCCACATCT 
CAGGCCACCA GGGGCAGGAC AAATAGGTCC TCTGTGAAGA CCCCTGAAAC AGTTGTCCCC 
ACAGCCCCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC CTGTTACCCC TGAGCCTACG 
TCTCAGGCTA CTAGGGGAAG AACAGATAGA TCCTCTGTCA AGACTCCTGA AACAGTTGTC 
CCCACAGCCC CTGAGCTACA GGCTTCCGCC TCCACAGACC AGCCTGTCAC CTCTGAGCCC 
ACATCTCGGA CCACTAGGGG AAGAAAAAAT CGGTCCTCTG TCAAGACCCC TGAAACAGTT 
GTGCCCGCAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAACCTGT CACCCCTGAG 
CCCACATCTC GGGCCACTAG GGGCAGGACA AATAGGTCCT CTGTCAAGAC CCCTGAATCA 
ATTGTCCCTA TAGCCCCTGA GCTTCAGCCT TCCACCTCCA GAAACCAGCT TGTCACCCCT 
GAGCCCACAT CTCGGGCCAC TAGGTGCAGG ACAAATAGGT CCTCTGTCAA GACCCCTGAG 
CCAGTTGTCC CCACAGCCCC TGAGCCCCAT CCTACCACCT CCACAGACCA GCCTGTCACC 
CCCAAGCTCA CATCTAGGGC CACTAGGAGA AAGACAAATA GGTCCTCTGT CAAGACTCCC 
AAACCAGTTG AACCAGCAGC CTCTGATCTT GAGCCTTTTA CCCCCACAGA CCAGTCCGTC 
ACCCCTGAGG CCATAGCTCA GGGTGGTCAG AGCAAAACAC TGAGGTCTTC CACAGTAAGA 
GCTATGCCGG TTCCTACCAC CCCTGAATTC CAATCTCCTG TCACCACAGA CCAGCCTATT 
TCCCCTGAGC CTATTACTCA ACCCAGTTGC ATCAAGAGGC AGAGAGCCGC TGGGAACCCT 
GGCTCCCTCG CAGCTCCCAT TGACCATAAG CCTTGCTCTG CACCCTTGGA ACCTAAATCC 
CAGGCCTCAA GGAACCAAAG ATGGGGAGCA GTGAGAGCAG CTGAATCCCT TACAGCCATT 
CCTGAGCCTG CCTCTCCCCA GCTTCTTGAG ACACCAATTC ATGCCTCCCA GATCCAAAAG 
GTGGAACCAG CAGGTAGATC TAGGTTCACC CCGGAGCTCC AGCCTAAGGC CTCTCAAAGC 
CGCAAGAGGT CTTTAGCTAC CATGGATTCA CCACCACATC AAAAACAGCC CCAAAGAGGG 
GAAGTCTCCC AGAAGACAGT GATTATCAAG GAAGAGGAAG AAGATACTGC AGAGAAGCCA 
GGGAAGGAAG AGGATGTCGT GACTCCAAAA CCAGGCAAGA GAAAGAGAGA CCAGGCAGAG 
GAGGAGCCCA ACAGAATACC AAGCCGCAGC CTCCGACGGA CCAAACTTAA CCAAGAATCA 
ACAGCCCCCA AAGTGCTCTT CACAGGAGTG GTGGATGCTC GGGGAGAGCG GGCTGTGCTG 
GCACTGGGGG GAAGTCTGGC TGGTTCAGCG GCAGAGGCTT CCCACCTGGT CACTGATCGC 
ATCCGCCGGA CAGTCAAGTT CCTGTGTGCC CTGGGGCGGG GAATCCCCAT TCTGTCCCTG 
GACTGGCTGC ATCAGTCCCG CAAGGCTGGT TTCTTCTTAC CCCCGGATGA ATATGTGGTG 
ACCGACCCTG AGCAAGAGAA GAACTTTGGC TTTAGCCTTC AAGACGCACT GAGCAGGGCT 
CGGGAGCGAA GGCTGCTAGA GGGCTATGAG ATCTATGTGA CCCCTGGAGT CCAGCCACCA 
CCACCTCAGA TGGGAGAGAT TATTAGCTGC TGTGGAGGCA CATACCTACC CAGCATGCCT 
CGGTCCTATA AGCCTCAGAG AGTTGTGATC ACATGCCCTC AGGACTTCCC TCATTGCTCC 
ATTCCACTAC GGGTTGGGCT GCCCCTCCTC TCGCCTGAGT TCCTGCTGAC TGGAGTGCTG 
AAGCAGGAAG CCAAGCCAGA GGCCTTTGTC CTCTCCCCTT TGGAGATGTC ATCCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515004.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513306.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OHu63099
Clone ID Related Accession (Same CDS sequence)	XM_011515004.3 , XM_011515004.2
Accession Version	XM_011515004.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011515004.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCATC GCCTGGATGT CTCTCTGCCC TTTGTCTCCC GGGGCCCTCT GACAGTAGAA 
GAGACACCCA GAGTACAGGG AGAAACTCAA CCCCAGAGGC TTCTGTTGGC TGAGGACTCG 
GAGGAGGAAG TAGATTTTCT TTCTGAAAGG CGTATGGTAA AAAAATCAAG GACCACATCT 
TCCTCTGTGA TAGTTCCAGA GAGTGATGAA GAGGGGCATT CCCCGGTCCT GGGCGGCCTT 
GGGCCGCCTT TTGCCTTCAA TTTGAACAGT GACACAGATG TGGAAGAAGG TCAGCAACCA 
GCCACAGAGG AGGCCTCCTC AGCTGCCAGA AGAGGTGCCA CTGTAGAGGC AAAGCAGTCT 
GAAGCTGAAG TTGTAACTGA AATCCAGCTT GAAAAGGATC AGCCTTTAGT GAAGGAGAGG 
GACAATGATA CAAAAGTCAA GAGGGGTGCA GGGAATGGGG TGGTTCCAGC TGGGGTGATT 
CTGGAGAGGA GCCAACCTCC TGGAGAGGAC AGTGACACAG ATGTGGATGA TGACAGCAGG 
CCTCCTGGAA GGCCAGCTGA GGTCCATTTG GAAAGGGCTC AGCCTTTTGG CTTCATCGAC 
AGCGACACTG ATGCGGAAGA AGAGAGGATC CCAGCAACCC CAGTTGTCAT TCCTATGAAG 
AAGAGGAAGA TCTTCCATGG AGTAGGTACA AGGGGTCCTG GAGCACCAGG CCTGGCCCAT 
CTGCAGGAGA GCCAGGCTGG TAGTGATACA GATGTGGAAG AAGGCAAGGC CCCACAGGCT 
GTCCCTCTGG AGAAAAGCCA AGCTTCCATG GTTATCAACA GCGATACAGA TGACGAGGAA 
GAAGTCTCAG CAGCGCTGAC TTTGGCACAT CTGAAAGAGA GCCAGCCTGC TATATGGAAC 
AGAGATGCAG AAGAGGACAT GCCCCAACGT GTGGTCCTTC TGCAGCGAAG CCAAACCACC 
ACTGAGAGAG ACAGTGACAC AGACGTGGAG GAGGAAGAGC TCCCAGTGGA AAATAGAGAA 
GCTGTCCTCA AGGATCACAC AAAGATTAGA GCCCTTGTTA GAGCACATTC AGAAAAGGAC 
CAACCTCCTT TTGGGGACAG TGATGACAGT GTGGAAGCAG ATAAGAGCTC ACCTGGGATC 
CACCTGGAGA GAAGCCAAGC CTCCACCACA GTGGACATCA ACACACAAGT GGAGAAGGAA 
GTCCCGCCAG GGTCAGCCAT TATACATATA AAGAAGCATC AGGTGTCTGT GGAGGGGACA 
AATCAAACAG ATGTGAAAGC AGTTGGGGGA CCAGCAAAGC TGCTTGTGGT ATCTCTAGAG 
GAAGCCTGGC CTCTGCATGG GGACTGTGAA ACAGATGCAG AGGAGGGCAC CTCCCTAACA 
GCCTCAGTAG TTGCAGATGT AAGAAAGAGC CAGCTTCCAG CAGAAGGGGA TGCTGGGGCA 
GAGTGGGCTG CAGCTGTTCT TAAGCAGGAG AGAGCTCATG AGGTGGGGGC CCAGGGTGGG 
CCACCTGTGG CACAAGTGGA GCAGGACCTC CCTATCTCAA GAGAGAACCT CACAGATCTG 
GTGGTGGACA CAGACACTCT AGGGGAATCC ACCCAGCCAC AGAGAGAGGG AGCCCAGGTC 
CCCACAGGAA GGGAGAGAGA ACAACATGTG GGTGGGACCA AGGACTCTGA AGACAACTAT 
GGTGATTCTG AAGATCTGGA CCTACAAGCT ACCCAGTGCT TTCTGGAGAA TCAGGGCCTG 
GAAGCAGTCC AGAGCATGGA GGATGAACCT ACCCAGGCCT TCATGTTGAC TCCACCCCAA 
GAGCTTGGCC CTTCCCATTG CAGCTTCCAG ACAACAGGTA CCCTAGATGA ACCATGGGAG 
GTCCTGGCTA CACAGCCATT CTGTCTGAGA GAGTCTGAGG ACTCTGAGAC CCAGCCTTTT 
GACACGCACC TTGAGGCCTA TGGACCTTGC CTGTCTCCAC CTAGGGCAAT ACCAGGAGAC 
CAACATCCAG AGAGCCCAGT TCACACAGAG CCAATGGGGA TTCAAGGCAG AGGGAGGCAG 
ACTGTGGATA AAGTCATGGG TATACCAAAA GAAACAGCAG AGAGGGTGGG CCCTGAGAGA 
GGGCCATTGG AGAGAGAAAC TGAGAAACTG CTACCAGAAA GACAGACAGA TGTGACAGGA 
GAGGAAGAAT TAACCAAGGG GAAACAGGAC AGAGAACAAA AACAGTTGTT AGCTAGAGAC 
ACCCAGAGAC AAGAATCTGA CAAAAATGGG GAAAGTGCAA GTCCTGAAAG AGATAGGGAG 
AGTTTGAAGG TAGAAATTGA GACATCTGAG GAAATACAAG AGAAACAAGT ACAGAAGCAG 
ACCCTTCCAA GCAAAGCATT TGAGAGAGAA GTAGAGAGAC CAGTAGCAAA CAGAGAGTGC 
GATCCAGCCG AGTTAGAAGA GAAGGTGCCC AAAGTGATCC TGGAGAGAGA TACACAGAGA 
GGGGAGCCAG AGGGAGGGAG CCAGGACCAG AAAGGGCAGG CCTCCAGCCC AACACCAGAG 
CCTGGGGTGG GGGCGGGGGA CCTTCCGGGA CCTACCTCAG CCCCCGTACC TTCTGGGAGC 
CAGTCAGGTG GAAGGGGATC CCCAGTGAGC CCCAGGAGGC ATCAGAAAGG CCTCCTGAAT 
TGCAAGATGC CACCTGCTGA GAAGGCTTCC AGGATCAGAG CTGCTGAGAA GGTTTCCAGG 
GGCGATCAGG AATCTCCAGA TGCTTGTCTG CCTCCTACAG TACCTGAAGC CCCAGCCCCA 
CCCCAAAAGC CCCTTAACTC TCAGAGCCAG AAACATCTTG CACCTCCGCC CCTTCTTTCT 
CCCCTTTTAC CTTCTATCAA GCCAACCGTT CGTAAGACCA GGCAAGATGG GAGTCAGGAA 
GCTCCAGAGG CTCCCTTGTC CTCAGAGCTG GAGCCTTTCC ACCCAAAGCC TAAAATTAGA 
ACTCGGAAGT CCTCCAGAAT GACACCCTTT CCAGCTACCT CTGCTGCCCC TGAGCCCCAC 
CCTTCCACCT CCACAGCCCA GCCAGTCACT CCCAAGCCCA CATCTCAGGC CACTAGGAGC 
AGGACAAATA GGTCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACCTCTGAGC CCACATCTCA GGTTACTAGG 
GGAAGAAAAA GTAGATCCTC TGTCAAGACC CCTGAAACAG TTGTGCCCAC AGCCCTTGAG 
CTCCAGCCTT CCACCTCCAC CGACCGACCT GTCACCTCTG AACCCACCTC TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CCTGTCACTT CTGAGCCCAC ATATCAGGCT 
ACTAGGGGAA GAAAAAATAG ATCCTCTGTC AAGACCCCTG AACCAGTTGT GCCCACAGCC 
CCTGAGCTCC GGCCTTCCAC CTCCACAGAC CGACCTGTCA CCCCCAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTCAAGACCC CTGAAACAGT TGTCCCCACA 
GCCCCTGAGC TCCAGATTTC CACCTCCACA GACCAACCTG TCACCCCTAA GCCCACATCT 
CGGACCACTA GGAGCAGGAC AAATATGTCC TCTGTGAAGA ACCCTGAATC AACTGTCCCT 
ATAGCCCCTG AGCTCCCACC TTCCACCTCC ACAGAGCAGC CTGTCACCCC TGAGCCCACA 
TCTCGGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGGCA AGACCCCTGA AACACTTGTC 
CCCACAGCCC CTAAGCTCGA GCCTTCCACT TCCACAGACC AACCTGTCAC TCCTGAGCCC 
ACATCTCAGG CCACCAGGGG CAGGACAAAT AGGTCCTCTG TGAAGACCCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT TACCCCTGAG 
CCTACGTCTC AGGCTACTAG GGGAAGAACA GATAGATCCT CTGTCAAGAC TCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTACAGGCT TCCGCCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCGGACCAC TAGGGGAAGA AAAAATCGGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCGCAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA ACCTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGGGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAATCAATTG TCCCTATAGC CCCTGAGCTT CAGCCTTCCA CCTCCAGAAA CCAGCTTGTC 
ACCCCTGAGC CCACATCTCG GGCCACTAGG TGCAGGACAA ATAGGTCCTC TGTCAAGACC 
CCTGAGCCAG TTGTCCCCAC AGCCCCTGAG CCCCATCCTA CCACCTCCAC AGACCAGCCT 
GTCACCCCCA AGCTCACATC TAGGGCCACT AGGAGAAAGA CAAATAGGTC CTCTGTCAAG 
ACTCCCAAAC CAGTTGAACC AGCAGCCTCT GATCTTGAGC CTTTTACCCC CACAGACCAG 
TCCGTCACCC CTGAGGCCAT AGCTCAGGGT GGTCAGAGCA AAACACTGAG GTCTTCCACA 
GTAAGAGCTA TGCCGGTTCC TACCACCCCT GAATTCCAAT CTCCTGTCAC CACAGACCAG 
CCTATTTCCC CTGAGCCTAT TACTCAACCC AGTTGCATCA AGAGGCAGAG AGCCGCTGGG 
AACCCTGGCT CCCTCGCAGC TCCCATTGAC CATAAGCCTT GCTCTGCACC CTTGGAACCT 
AAATCCCAGG CCTCAAGGAA CCAAAGATGG GGAGCAGTGA GAGCAGCTGA ATCCCTTACA 
GCCATTCCTG AGCCTGCCTC TCCCCAGCTT CTTGAGACAC CAATTCATGC CTCCCAGATC 
CAAAAGGTGG AACCAGCAGG TAGATCTAGG TTCACCCCGG AGCTCCAGCC TAAGGCCTCT 
CAAAGCCGCA AGAGGTCTTT AGCTACCATG GATTCACCAC CACATCAAAA ACAGCCCCAA 
AGAGGGGAAG TCTCCCAGAA GACAGTGATT ATCAAGGAAG AGGAAGAAGA TACTGCAGAG 
AAGCCAGGGA AGGAAGAGGA TGTCGTGACT CCAAAACCAG GCAAGAGAAA GAGAGACCAG 
GCAGAGGAGG AGCCCAACAG AATACCAAGC CGCAGCCTCC GACGGACCAA ACTTAACCAA 
GAATCAACAG CCCCCAAAGT GCTCTTCACA GGAGTGGTGG ATGCTCGGGG AGAGCGGGCT 
GTGCTGGCAC TGGGGGGAAG TCTGGCTGGT TCAGCGGCAG AGGCTTCCCA CCTGGTCACT 
GATCGCATCC GCCGGACAGT CAAGTTCCTG TGTGCCCTGG GGCGGGGAAT CCCCATTCTG 
TCCCTGGACT GGCTGCATCA GTCCCGCAAG GCTGGTTTCT TCTTACCCCC GGATGAATAT 
GTGGTGACCG ACCCTGAGCA AGAGAAGAAC TTTGGCTTTA GCCTTCAAGA CGCACTGAGC 
AGGGCTCGGG AGCGAAGGCT GCTAGAGGGC TATGAGATCT ATGTGACCCC TGGAGTCCAG 
CCACCACCAC CTCAGATGGG AGAGATTATT AGCTGCTGTG GAGGCACATA CCTACCCAGC 
ATGCCTCGGT CCTATAAGCC TCAGAGAGTT GTGATCACAT GCCCTCAGGA CTTCCCTCAT 
TGCTCCATTC CACTACGGGT TGGGCTGCCC CTCCTCTCGC CTGAGTTCCT GCTGACTGGA 
GTGCTGAAGC AGGAAGCCAA GCCAGAGGCC TTTGTCCTCT CCCCTTTGGA GATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513306.1
CDS	440..6325
Translation	MYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515004.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515004.3

ORF Insert Sequence:

ATGTACCATC GCCTGGATGT CTCTCTGCCC TTTGTCTCCC GGGGCCCTCT GACAGTAGAA 
GAGACACCCA GAGTACAGGG AGAAACTCAA CCCCAGAGGC TTCTGTTGGC TGAGGACTCG 
GAGGAGGAAG TAGATTTTCT TTCTGAAAGG CGTATGGTAA AAAAATCAAG GACCACATCT 
TCCTCTGTGA TAGTTCCAGA GAGTGATGAA GAGGGGCATT CCCCGGTCCT GGGCGGCCTT 
GGGCCGCCTT TTGCCTTCAA TTTGAACAGT GACACAGATG TGGAAGAAGG TCAGCAACCA 
GCCACAGAGG AGGCCTCCTC AGCTGCCAGA AGAGGTGCCA CTGTAGAGGC AAAGCAGTCT 
GAAGCTGAAG TTGTAACTGA AATCCAGCTT GAAAAGGATC AGCCTTTAGT GAAGGAGAGG 
GACAATGATA CAAAAGTCAA GAGGGGTGCA GGGAATGGGG TGGTTCCAGC TGGGGTGATT 
CTGGAGAGGA GCCAACCTCC TGGAGAGGAC AGTGACACAG ATGTGGATGA TGACAGCAGG 
CCTCCTGGAA GGCCAGCTGA GGTCCATTTG GAAAGGGCTC AGCCTTTTGG CTTCATCGAC 
AGCGACACTG ATGCGGAAGA AGAGAGGATC CCAGCAACCC CAGTTGTCAT TCCTATGAAG 
AAGAGGAAGA TCTTCCATGG AGTAGGTACA AGGGGTCCTG GAGCACCAGG CCTGGCCCAT 
CTGCAGGAGA GCCAGGCTGG TAGTGATACA GATGTGGAAG AAGGCAAGGC CCCACAGGCT 
GTCCCTCTGG AGAAAAGCCA AGCTTCCATG GTTATCAACA GCGATACAGA TGACGAGGAA 
GAAGTCTCAG CAGCGCTGAC TTTGGCACAT CTGAAAGAGA GCCAGCCTGC TATATGGAAC 
AGAGATGCAG AAGAGGACAT GCCCCAACGT GTGGTCCTTC TGCAGCGAAG CCAAACCACC 
ACTGAGAGAG ACAGTGACAC AGACGTGGAG GAGGAAGAGC TCCCAGTGGA AAATAGAGAA 
GCTGTCCTCA AGGATCACAC AAAGATTAGA GCCCTTGTTA GAGCACATTC AGAAAAGGAC 
CAACCTCCTT TTGGGGACAG TGATGACAGT GTGGAAGCAG ATAAGAGCTC ACCTGGGATC 
CACCTGGAGA GAAGCCAAGC CTCCACCACA GTGGACATCA ACACACAAGT GGAGAAGGAA 
GTCCCGCCAG GGTCAGCCAT TATACATATA AAGAAGCATC AGGTGTCTGT GGAGGGGACA 
AATCAAACAG ATGTGAAAGC AGTTGGGGGA CCAGCAAAGC TGCTTGTGGT ATCTCTAGAG 
GAAGCCTGGC CTCTGCATGG GGACTGTGAA ACAGATGCAG AGGAGGGCAC CTCCCTAACA 
GCCTCAGTAG TTGCAGATGT AAGAAAGAGC CAGCTTCCAG CAGAAGGGGA TGCTGGGGCA 
GAGTGGGCTG CAGCTGTTCT TAAGCAGGAG AGAGCTCATG AGGTGGGGGC CCAGGGTGGG 
CCACCTGTGG CACAAGTGGA GCAGGACCTC CCTATCTCAA GAGAGAACCT CACAGATCTG 
GTGGTGGACA CAGACACTCT AGGGGAATCC ACCCAGCCAC AGAGAGAGGG AGCCCAGGTC 
CCCACAGGAA GGGAGAGAGA ACAACATGTG GGTGGGACCA AGGACTCTGA AGACAACTAT 
GGTGATTCTG AAGATCTGGA CCTACAAGCT ACCCAGTGCT TTCTGGAGAA TCAGGGCCTG 
GAAGCAGTCC AGAGCATGGA GGATGAACCT ACCCAGGCCT TCATGTTGAC TCCACCCCAA 
GAGCTTGGCC CTTCCCATTG CAGCTTCCAG ACAACAGGTA CCCTAGATGA ACCATGGGAG 
GTCCTGGCTA CACAGCCATT CTGTCTGAGA GAGTCTGAGG ACTCTGAGAC CCAGCCTTTT 
GACACGCACC TTGAGGCCTA TGGACCTTGC CTGTCTCCAC CTAGGGCAAT ACCAGGAGAC 
CAACATCCAG AGAGCCCAGT TCACACAGAG CCAATGGGGA TTCAAGGCAG AGGGAGGCAG 
ACTGTGGATA AAGTCATGGG TATACCAAAA GAAACAGCAG AGAGGGTGGG CCCTGAGAGA 
GGGCCATTGG AGAGAGAAAC TGAGAAACTG CTACCAGAAA GACAGACAGA TGTGACAGGA 
GAGGAAGAAT TAACCAAGGG GAAACAGGAC AGAGAACAAA AACAGTTGTT AGCTAGAGAC 
ACCCAGAGAC AAGAATCTGA CAAAAATGGG GAAAGTGCAA GTCCTGAAAG AGATAGGGAG 
AGTTTGAAGG TAGAAATTGA GACATCTGAG GAAATACAAG AGAAACAAGT ACAGAAGCAG 
ACCCTTCCAA GCAAAGCATT TGAGAGAGAA GTAGAGAGAC CAGTAGCAAA CAGAGAGTGC 
GATCCAGCCG AGTTAGAAGA GAAGGTGCCC AAAGTGATCC TGGAGAGAGA TACACAGAGA 
GGGGAGCCAG AGGGAGGGAG CCAGGACCAG AAAGGGCAGG CCTCCAGCCC AACACCAGAG 
CCTGGGGTGG GGGCGGGGGA CCTTCCGGGA CCTACCTCAG CCCCCGTACC TTCTGGGAGC 
CAGTCAGGTG GAAGGGGATC CCCAGTGAGC CCCAGGAGGC ATCAGAAAGG CCTCCTGAAT 
TGCAAGATGC CACCTGCTGA GAAGGCTTCC AGGATCAGAG CTGCTGAGAA GGTTTCCAGG 
GGCGATCAGG AATCTCCAGA TGCTTGTCTG CCTCCTACAG TACCTGAAGC CCCAGCCCCA 
CCCCAAAAGC CCCTTAACTC TCAGAGCCAG AAACATCTTG CACCTCCGCC CCTTCTTTCT 
CCCCTTTTAC CTTCTATCAA GCCAACCGTT CGTAAGACCA GGCAAGATGG GAGTCAGGAA 
GCTCCAGAGG CTCCCTTGTC CTCAGAGCTG GAGCCTTTCC ACCCAAAGCC TAAAATTAGA 
ACTCGGAAGT CCTCCAGAAT GACACCCTTT CCAGCTACCT CTGCTGCCCC TGAGCCCCAC 
CCTTCCACCT CCACAGCCCA GCCAGTCACT CCCAAGCCCA CATCTCAGGC CACTAGGAGC 
AGGACAAATA GGTCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACCTCTGAGC CCACATCTCA GGTTACTAGG 
GGAAGAAAAA GTAGATCCTC TGTCAAGACC CCTGAAACAG TTGTGCCCAC AGCCCTTGAG 
CTCCAGCCTT CCACCTCCAC CGACCGACCT GTCACCTCTG AACCCACCTC TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CCTGTCACTT CTGAGCCCAC ATATCAGGCT 
ACTAGGGGAA GAAAAAATAG ATCCTCTGTC AAGACCCCTG AACCAGTTGT GCCCACAGCC 
CCTGAGCTCC GGCCTTCCAC CTCCACAGAC CGACCTGTCA CCCCCAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTCAAGACCC CTGAAACAGT TGTCCCCACA 
GCCCCTGAGC TCCAGATTTC CACCTCCACA GACCAACCTG TCACCCCTAA GCCCACATCT 
CGGACCACTA GGAGCAGGAC AAATATGTCC TCTGTGAAGA ACCCTGAATC AACTGTCCCT 
ATAGCCCCTG AGCTCCCACC TTCCACCTCC ACAGAGCAGC CTGTCACCCC TGAGCCCACA 
TCTCGGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGGCA AGACCCCTGA AACACTTGTC 
CCCACAGCCC CTAAGCTCGA GCCTTCCACT TCCACAGACC AACCTGTCAC TCCTGAGCCC 
ACATCTCAGG CCACCAGGGG CAGGACAAAT AGGTCCTCTG TGAAGACCCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT TACCCCTGAG 
CCTACGTCTC AGGCTACTAG GGGAAGAACA GATAGATCCT CTGTCAAGAC TCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTACAGGCT TCCGCCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCGGACCAC TAGGGGAAGA AAAAATCGGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCGCAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA ACCTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGGGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAATCAATTG TCCCTATAGC CCCTGAGCTT CAGCCTTCCA CCTCCAGAAA CCAGCTTGTC 
ACCCCTGAGC CCACATCTCG GGCCACTAGG TGCAGGACAA ATAGGTCCTC TGTCAAGACC 
CCTGAGCCAG TTGTCCCCAC AGCCCCTGAG CCCCATCCTA CCACCTCCAC AGACCAGCCT 
GTCACCCCCA AGCTCACATC TAGGGCCACT AGGAGAAAGA CAAATAGGTC CTCTGTCAAG 
ACTCCCAAAC CAGTTGAACC AGCAGCCTCT GATCTTGAGC CTTTTACCCC CACAGACCAG 
TCCGTCACCC CTGAGGCCAT AGCTCAGGGT GGTCAGAGCA AAACACTGAG GTCTTCCACA 
GTAAGAGCTA TGCCGGTTCC TACCACCCCT GAATTCCAAT CTCCTGTCAC CACAGACCAG 
CCTATTTCCC CTGAGCCTAT TACTCAACCC AGTTGCATCA AGAGGCAGAG AGCCGCTGGG 
AACCCTGGCT CCCTCGCAGC TCCCATTGAC CATAAGCCTT GCTCTGCACC CTTGGAACCT 
AAATCCCAGG CCTCAAGGAA CCAAAGATGG GGAGCAGTGA GAGCAGCTGA ATCCCTTACA 
GCCATTCCTG AGCCTGCCTC TCCCCAGCTT CTTGAGACAC CAATTCATGC CTCCCAGATC 
CAAAAGGTGG AACCAGCAGG TAGATCTAGG TTCACCCCGG AGCTCCAGCC TAAGGCCTCT 
CAAAGCCGCA AGAGGTCTTT AGCTACCATG GATTCACCAC CACATCAAAA ACAGCCCCAA 
AGAGGGGAAG TCTCCCAGAA GACAGTGATT ATCAAGGAAG AGGAAGAAGA TACTGCAGAG 
AAGCCAGGGA AGGAAGAGGA TGTCGTGACT CCAAAACCAG GCAAGAGAAA GAGAGACCAG 
GCAGAGGAGG AGCCCAACAG AATACCAAGC CGCAGCCTCC GACGGACCAA ACTTAACCAA 
GAATCAACAG CCCCCAAAGT GCTCTTCACA GGAGTGGTGG ATGCTCGGGG AGAGCGGGCT 
GTGCTGGCAC TGGGGGGAAG TCTGGCTGGT TCAGCGGCAG AGGCTTCCCA CCTGGTCACT 
GATCGCATCC GCCGGACAGT CAAGTTCCTG TGTGCCCTGG GGCGGGGAAT CCCCATTCTG 
TCCCTGGACT GGCTGCATCA GTCCCGCAAG GCTGGTTTCT TCTTACCCCC GGATGAATAT 
GTGGTGACCG ACCCTGAGCA AGAGAAGAAC TTTGGCTTTA GCCTTCAAGA CGCACTGAGC 
AGGGCTCGGG AGCGAAGGCT GCTAGAGGGC TATGAGATCT ATGTGACCCC TGGAGTCCAG 
CCACCACCAC CTCAGATGGG AGAGATTATT AGCTGCTGTG GAGGCACATA CCTACCCAGC 
ATGCCTCGGT CCTATAAGCC TCAGAGAGTT GTGATCACAT GCCCTCAGGA CTTCCCTCAT 
TGCTCCATTC CACTACGGGT TGGGCTGCCC CTCCTCTCGC CTGAGTTCCT GCTGACTGGA 
GTGCTGAAGC AGGAAGCCAA GCCAGAGGCC TTTGTCCTCT CCCCTTTGGA GATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_011515004.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011513306.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OHu63099
Clone ID Related Accession (Same CDS sequence)	XM_011515004.3 , XM_011515004.2
Accession Version	XM_011515004.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5886bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCATC GCCTGGATGT CTCTCTGCCC TTTGTCTCCC GGGGCCCTCT GACAGTAGAA 
GAGACACCCA GAGTACAGGG AGAAACTCAA CCCCAGAGGC TTCTGTTGGC TGAGGACTCG 
GAGGAGGAAG TAGATTTTCT TTCTGAAAGG CGTATGGTAA AAAAATCAAG GACCACATCT 
TCCTCTGTGA TAGTTCCAGA GAGTGATGAA GAGGGGCATT CCCCGGTCCT GGGCGGCCTT 
GGGCCGCCTT TTGCCTTCAA TTTGAACAGT GACACAGATG TGGAAGAAGG TCAGCAACCA 
GCCACAGAGG AGGCCTCCTC AGCTGCCAGA AGAGGTGCCA CTGTAGAGGC AAAGCAGTCT 
GAAGCTGAAG TTGTAACTGA AATCCAGCTT GAAAAGGATC AGCCTTTAGT GAAGGAGAGG 
GACAATGATA CAAAAGTCAA GAGGGGTGCA GGGAATGGGG TGGTTCCAGC TGGGGTGATT 
CTGGAGAGGA GCCAACCTCC TGGAGAGGAC AGTGACACAG ATGTGGATGA TGACAGCAGG 
CCTCCTGGAA GGCCAGCTGA GGTCCATTTG GAAAGGGCTC AGCCTTTTGG CTTCATCGAC 
AGCGACACTG ATGCGGAAGA AGAGAGGATC CCAGCAACCC CAGTTGTCAT TCCTATGAAG 
AAGAGGAAGA TCTTCCATGG AGTAGGTACA AGGGGTCCTG GAGCACCAGG CCTGGCCCAT 
CTGCAGGAGA GCCAGGCTGG TAGTGATACA GATGTGGAAG AAGGCAAGGC CCCACAGGCT 
GTCCCTCTGG AGAAAAGCCA AGCTTCCATG GTTATCAACA GCGATACAGA TGACGAGGAA 
GAAGTCTCAG CAGCGCTGAC TTTGGCACAT CTGAAAGAGA GCCAGCCTGC TATATGGAAC 
AGAGATGCAG AAGAGGACAT GCCCCAACGT GTGGTCCTTC TGCAGCGAAG CCAAACCACC 
ACTGAGAGAG ACAGTGACAC AGACGTGGAG GAGGAAGAGC TCCCAGTGGA AAATAGAGAA 
GCTGTCCTCA AGGATCACAC AAAGATTAGA GCCCTTGTTA GAGCACATTC AGAAAAGGAC 
CAACCTCCTT TTGGGGACAG TGATGACAGT GTGGAAGCAG ATAAGAGCTC ACCTGGGATC 
CACCTGGAGA GAAGCCAAGC CTCCACCACA GTGGACATCA ACACACAAGT GGAGAAGGAA 
GTCCCGCCAG GGTCAGCCAT TATACATATA AAGAAGCATC AGGTGTCTGT GGAGGGGACA 
AATCAAACAG ATGTGAAAGC AGTTGGGGGA CCAGCAAAGC TGCTTGTGGT ATCTCTAGAG 
GAAGCCTGGC CTCTGCATGG GGACTGTGAA ACAGATGCAG AGGAGGGCAC CTCCCTAACA 
GCCTCAGTAG TTGCAGATGT AAGAAAGAGC CAGCTTCCAG CAGAAGGGGA TGCTGGGGCA 
GAGTGGGCTG CAGCTGTTCT TAAGCAGGAG AGAGCTCATG AGGTGGGGGC CCAGGGTGGG 
CCACCTGTGG CACAAGTGGA GCAGGACCTC CCTATCTCAA GAGAGAACCT CACAGATCTG 
GTGGTGGACA CAGACACTCT AGGGGAATCC ACCCAGCCAC AGAGAGAGGG AGCCCAGGTC 
CCCACAGGAA GGGAGAGAGA ACAACATGTG GGTGGGACCA AGGACTCTGA AGACAACTAT 
GGTGATTCTG AAGATCTGGA CCTACAAGCT ACCCAGTGCT TTCTGGAGAA TCAGGGCCTG 
GAAGCAGTCC AGAGCATGGA GGATGAACCT ACCCAGGCCT TCATGTTGAC TCCACCCCAA 
GAGCTTGGCC CTTCCCATTG CAGCTTCCAG ACAACAGGTA CCCTAGATGA ACCATGGGAG 
GTCCTGGCTA CACAGCCATT CTGTCTGAGA GAGTCTGAGG ACTCTGAGAC CCAGCCTTTT 
GACACGCACC TTGAGGCCTA TGGACCTTGC CTGTCTCCAC CTAGGGCAAT ACCAGGAGAC 
CAACATCCAG AGAGCCCAGT TCACACAGAG CCAATGGGGA TTCAAGGCAG AGGGAGGCAG 
ACTGTGGATA AAGTCATGGG TATACCAAAA GAAACAGCAG AGAGGGTGGG CCCTGAGAGA 
GGGCCATTGG AGAGAGAAAC TGAGAAACTG CTACCAGAAA GACAGACAGA TGTGACAGGA 
GAGGAAGAAT TAACCAAGGG GAAACAGGAC AGAGAACAAA AACAGTTGTT AGCTAGAGAC 
ACCCAGAGAC AAGAATCTGA CAAAAATGGG GAAAGTGCAA GTCCTGAAAG AGATAGGGAG 
AGTTTGAAGG TAGAAATTGA GACATCTGAG GAAATACAAG AGAAACAAGT ACAGAAGCAG 
ACCCTTCCAA GCAAAGCATT TGAGAGAGAA GTAGAGAGAC CAGTAGCAAA CAGAGAGTGC 
GATCCAGCCG AGTTAGAAGA GAAGGTGCCC AAAGTGATCC TGGAGAGAGA TACACAGAGA 
GGGGAGCCAG AGGGAGGGAG CCAGGACCAG AAAGGGCAGG CCTCCAGCCC AACACCAGAG 
CCTGGGGTGG GGGCGGGGGA CCTTCCGGGA CCTACCTCAG CCCCCGTACC TTCTGGGAGC 
CAGTCAGGTG GAAGGGGATC CCCAGTGAGC CCCAGGAGGC ATCAGAAAGG CCTCCTGAAT 
TGCAAGATGC CACCTGCTGA GAAGGCTTCC AGGATCAGAG CTGCTGAGAA GGTTTCCAGG 
GGCGATCAGG AATCTCCAGA TGCTTGTCTG CCTCCTACAG TACCTGAAGC CCCAGCCCCA 
CCCCAAAAGC CCCTTAACTC TCAGAGCCAG AAACATCTTG CACCTCCGCC CCTTCTTTCT 
CCCCTTTTAC CTTCTATCAA GCCAACCGTT CGTAAGACCA GGCAAGATGG GAGTCAGGAA 
GCTCCAGAGG CTCCCTTGTC CTCAGAGCTG GAGCCTTTCC ACCCAAAGCC TAAAATTAGA 
ACTCGGAAGT CCTCCAGAAT GACACCCTTT CCAGCTACCT CTGCTGCCCC TGAGCCCCAC 
CCTTCCACCT CCACAGCCCA GCCAGTCACT CCCAAGCCCA CATCTCAGGC CACTAGGAGC 
AGGACAAATA GGTCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACCTCTGAGC CCACATCTCA GGTTACTAGG 
GGAAGAAAAA GTAGATCCTC TGTCAAGACC CCTGAAACAG TTGTGCCCAC AGCCCTTGAG 
CTCCAGCCTT CCACCTCCAC CGACCGACCT GTCACCTCTG AACCCACCTC TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CCTGTCACTT CTGAGCCCAC ATATCAGGCT 
ACTAGGGGAA GAAAAAATAG ATCCTCTGTC AAGACCCCTG AACCAGTTGT GCCCACAGCC 
CCTGAGCTCC GGCCTTCCAC CTCCACAGAC CGACCTGTCA CCCCCAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTCAAGACCC CTGAAACAGT TGTCCCCACA 
GCCCCTGAGC TCCAGATTTC CACCTCCACA GACCAACCTG TCACCCCTAA GCCCACATCT 
CGGACCACTA GGAGCAGGAC AAATATGTCC TCTGTGAAGA ACCCTGAATC AACTGTCCCT 
ATAGCCCCTG AGCTCCCACC TTCCACCTCC ACAGAGCAGC CTGTCACCCC TGAGCCCACA 
TCTCGGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGGCA AGACCCCTGA AACACTTGTC 
CCCACAGCCC CTAAGCTCGA GCCTTCCACT TCCACAGACC AACCTGTCAC TCCTGAGCCC 
ACATCTCAGG CCACCAGGGG CAGGACAAAT AGGTCCTCTG TGAAGACCCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT TACCCCTGAG 
CCTACGTCTC AGGCTACTAG GGGAAGAACA GATAGATCCT CTGTCAAGAC TCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTACAGGCT TCCGCCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCGGACCAC TAGGGGAAGA AAAAATCGGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCGCAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA ACCTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGGGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAATCAATTG TCCCTATAGC CCCTGAGCTT CAGCCTTCCA CCTCCAGAAA CCAGCTTGTC 
ACCCCTGAGC CCACATCTCG GGCCACTAGG TGCAGGACAA ATAGGTCCTC TGTCAAGACC 
CCTGAGCCAG TTGTCCCCAC AGCCCCTGAG CCCCATCCTA CCACCTCCAC AGACCAGCCT 
GTCACCCCCA AGCTCACATC TAGGGCCACT AGGAGAAAGA CAAATAGGTC CTCTGTCAAG 
ACTCCCAAAC CAGTTGAACC AGCAGCCTCT GATCTTGAGC CTTTTACCCC CACAGACCAG 
TCCGTCACCC CTGAGGCCAT AGCTCAGGGT GGTCAGAGCA AAACACTGAG GTCTTCCACA 
GTAAGAGCTA TGCCGGTTCC TACCACCCCT GAATTCCAAT CTCCTGTCAC CACAGACCAG 
CCTATTTCCC CTGAGCCTAT TACTCAACCC AGTTGCATCA AGAGGCAGAG AGCCGCTGGG 
AACCCTGGCT CCCTCGCAGC TCCCATTGAC CATAAGCCTT GCTCTGCACC CTTGGAACCT 
AAATCCCAGG CCTCAAGGAA CCAAAGATGG GGAGCAGTGA GAGCAGCTGA ATCCCTTACA 
GCCATTCCTG AGCCTGCCTC TCCCCAGCTT CTTGAGACAC CAATTCATGC CTCCCAGATC 
CAAAAGGTGG AACCAGCAGG TAGATCTAGG TTCACCCCGG AGCTCCAGCC TAAGGCCTCT 
CAAAGCCGCA AGAGGTCTTT AGCTACCATG GATTCACCAC CACATCAAAA ACAGCCCCAA 
AGAGGGGAAG TCTCCCAGAA GACAGTGATT ATCAAGGAAG AGGAAGAAGA TACTGCAGAG 
AAGCCAGGGA AGGAAGAGGA TGTCGTGACT CCAAAACCAG GCAAGAGAAA GAGAGACCAG 
GCAGAGGAGG AGCCCAACAG AATACCAAGC CGCAGCCTCC GACGGACCAA ACTTAACCAA 
GAATCAACAG CCCCCAAAGT GCTCTTCACA GGAGTGGTGG ATGCTCGGGG AGAGCGGGCT 
GTGCTGGCAC TGGGGGGAAG TCTGGCTGGT TCAGCGGCAG AGGCTTCCCA CCTGGTCACT 
GATCGCATCC GCCGGACAGT CAAGTTCCTG TGTGCCCTGG GGCGGGGAAT CCCCATTCTG 
TCCCTGGACT GGCTGCATCA GTCCCGCAAG GCTGGTTTCT TCTTACCCCC GGATGAATAT 
GTGGTGACCG ACCCTGAGCA AGAGAAGAAC TTTGGCTTTA GCCTTCAAGA CGCACTGAGC 
AGGGCTCGGG AGCGAAGGCT GCTAGAGGGC TATGAGATCT ATGTGACCCC TGGAGTCCAG 
CCACCACCAC CTCAGATGGG AGAGATTATT AGCTGCTGTG GAGGCACATA CCTACCCAGC 
ATGCCTCGGT CCTATAAGCC TCAGAGAGTT GTGATCACAT GCCCTCAGGA CTTCCCTCAT 
TGCTCCATTC CACTACGGGT TGGGCTGCCC CTCCTCTCGC CTGAGTTCCT GCTGACTGGA 
GTGCTGAAGC AGGAAGCCAA GCCAGAGGCC TTTGTCCTCT CCCCTTTGGA GATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011513306.1
CDS	440..6325
Translation	MYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_011515004.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011515004.2

ORF Insert Sequence:

ATGTACCATC GCCTGGATGT CTCTCTGCCC TTTGTCTCCC GGGGCCCTCT GACAGTAGAA 
GAGACACCCA GAGTACAGGG AGAAACTCAA CCCCAGAGGC TTCTGTTGGC TGAGGACTCG 
GAGGAGGAAG TAGATTTTCT TTCTGAAAGG CGTATGGTAA AAAAATCAAG GACCACATCT 
TCCTCTGTGA TAGTTCCAGA GAGTGATGAA GAGGGGCATT CCCCGGTCCT GGGCGGCCTT 
GGGCCGCCTT TTGCCTTCAA TTTGAACAGT GACACAGATG TGGAAGAAGG TCAGCAACCA 
GCCACAGAGG AGGCCTCCTC AGCTGCCAGA AGAGGTGCCA CTGTAGAGGC AAAGCAGTCT 
GAAGCTGAAG TTGTAACTGA AATCCAGCTT GAAAAGGATC AGCCTTTAGT GAAGGAGAGG 
GACAATGATA CAAAAGTCAA GAGGGGTGCA GGGAATGGGG TGGTTCCAGC TGGGGTGATT 
CTGGAGAGGA GCCAACCTCC TGGAGAGGAC AGTGACACAG ATGTGGATGA TGACAGCAGG 
CCTCCTGGAA GGCCAGCTGA GGTCCATTTG GAAAGGGCTC AGCCTTTTGG CTTCATCGAC 
AGCGACACTG ATGCGGAAGA AGAGAGGATC CCAGCAACCC CAGTTGTCAT TCCTATGAAG 
AAGAGGAAGA TCTTCCATGG AGTAGGTACA AGGGGTCCTG GAGCACCAGG CCTGGCCCAT 
CTGCAGGAGA GCCAGGCTGG TAGTGATACA GATGTGGAAG AAGGCAAGGC CCCACAGGCT 
GTCCCTCTGG AGAAAAGCCA AGCTTCCATG GTTATCAACA GCGATACAGA TGACGAGGAA 
GAAGTCTCAG CAGCGCTGAC TTTGGCACAT CTGAAAGAGA GCCAGCCTGC TATATGGAAC 
AGAGATGCAG AAGAGGACAT GCCCCAACGT GTGGTCCTTC TGCAGCGAAG CCAAACCACC 
ACTGAGAGAG ACAGTGACAC AGACGTGGAG GAGGAAGAGC TCCCAGTGGA AAATAGAGAA 
GCTGTCCTCA AGGATCACAC AAAGATTAGA GCCCTTGTTA GAGCACATTC AGAAAAGGAC 
CAACCTCCTT TTGGGGACAG TGATGACAGT GTGGAAGCAG ATAAGAGCTC ACCTGGGATC 
CACCTGGAGA GAAGCCAAGC CTCCACCACA GTGGACATCA ACACACAAGT GGAGAAGGAA 
GTCCCGCCAG GGTCAGCCAT TATACATATA AAGAAGCATC AGGTGTCTGT GGAGGGGACA 
AATCAAACAG ATGTGAAAGC AGTTGGGGGA CCAGCAAAGC TGCTTGTGGT ATCTCTAGAG 
GAAGCCTGGC CTCTGCATGG GGACTGTGAA ACAGATGCAG AGGAGGGCAC CTCCCTAACA 
GCCTCAGTAG TTGCAGATGT AAGAAAGAGC CAGCTTCCAG CAGAAGGGGA TGCTGGGGCA 
GAGTGGGCTG CAGCTGTTCT TAAGCAGGAG AGAGCTCATG AGGTGGGGGC CCAGGGTGGG 
CCACCTGTGG CACAAGTGGA GCAGGACCTC CCTATCTCAA GAGAGAACCT CACAGATCTG 
GTGGTGGACA CAGACACTCT AGGGGAATCC ACCCAGCCAC AGAGAGAGGG AGCCCAGGTC 
CCCACAGGAA GGGAGAGAGA ACAACATGTG GGTGGGACCA AGGACTCTGA AGACAACTAT 
GGTGATTCTG AAGATCTGGA CCTACAAGCT ACCCAGTGCT TTCTGGAGAA TCAGGGCCTG 
GAAGCAGTCC AGAGCATGGA GGATGAACCT ACCCAGGCCT TCATGTTGAC TCCACCCCAA 
GAGCTTGGCC CTTCCCATTG CAGCTTCCAG ACAACAGGTA CCCTAGATGA ACCATGGGAG 
GTCCTGGCTA CACAGCCATT CTGTCTGAGA GAGTCTGAGG ACTCTGAGAC CCAGCCTTTT 
GACACGCACC TTGAGGCCTA TGGACCTTGC CTGTCTCCAC CTAGGGCAAT ACCAGGAGAC 
CAACATCCAG AGAGCCCAGT TCACACAGAG CCAATGGGGA TTCAAGGCAG AGGGAGGCAG 
ACTGTGGATA AAGTCATGGG TATACCAAAA GAAACAGCAG AGAGGGTGGG CCCTGAGAGA 
GGGCCATTGG AGAGAGAAAC TGAGAAACTG CTACCAGAAA GACAGACAGA TGTGACAGGA 
GAGGAAGAAT TAACCAAGGG GAAACAGGAC AGAGAACAAA AACAGTTGTT AGCTAGAGAC 
ACCCAGAGAC AAGAATCTGA CAAAAATGGG GAAAGTGCAA GTCCTGAAAG AGATAGGGAG 
AGTTTGAAGG TAGAAATTGA GACATCTGAG GAAATACAAG AGAAACAAGT ACAGAAGCAG 
ACCCTTCCAA GCAAAGCATT TGAGAGAGAA GTAGAGAGAC CAGTAGCAAA CAGAGAGTGC 
GATCCAGCCG AGTTAGAAGA GAAGGTGCCC AAAGTGATCC TGGAGAGAGA TACACAGAGA 
GGGGAGCCAG AGGGAGGGAG CCAGGACCAG AAAGGGCAGG CCTCCAGCCC AACACCAGAG 
CCTGGGGTGG GGGCGGGGGA CCTTCCGGGA CCTACCTCAG CCCCCGTACC TTCTGGGAGC 
CAGTCAGGTG GAAGGGGATC CCCAGTGAGC CCCAGGAGGC ATCAGAAAGG CCTCCTGAAT 
TGCAAGATGC CACCTGCTGA GAAGGCTTCC AGGATCAGAG CTGCTGAGAA GGTTTCCAGG 
GGCGATCAGG AATCTCCAGA TGCTTGTCTG CCTCCTACAG TACCTGAAGC CCCAGCCCCA 
CCCCAAAAGC CCCTTAACTC TCAGAGCCAG AAACATCTTG CACCTCCGCC CCTTCTTTCT 
CCCCTTTTAC CTTCTATCAA GCCAACCGTT CGTAAGACCA GGCAAGATGG GAGTCAGGAA 
GCTCCAGAGG CTCCCTTGTC CTCAGAGCTG GAGCCTTTCC ACCCAAAGCC TAAAATTAGA 
ACTCGGAAGT CCTCCAGAAT GACACCCTTT CCAGCTACCT CTGCTGCCCC TGAGCCCCAC 
CCTTCCACCT CCACAGCCCA GCCAGTCACT CCCAAGCCCA CATCTCAGGC CACTAGGAGC 
AGGACAAATA GGTCCTCTGT CAAGACCCCT GAACCAGTTG TCCCCACAGC CCCTGAGCTC 
CAGCCTTCCA CCTCCACAGA CCAGCCTGTC ACCTCTGAGC CCACATCTCA GGTTACTAGG 
GGAAGAAAAA GTAGATCCTC TGTCAAGACC CCTGAAACAG TTGTGCCCAC AGCCCTTGAG 
CTCCAGCCTT CCACCTCCAC CGACCGACCT GTCACCTCTG AACCCACCTC TCAGGCTACT 
AGGGGAAGAA AAAATAGATC CTCTGTCAAG ACCCCTGAAC CAGTTGTCCC CACAGCCCCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CCTGTCACTT CTGAGCCCAC ATATCAGGCT 
ACTAGGGGAA GAAAAAATAG ATCCTCTGTC AAGACCCCTG AACCAGTTGT GCCCACAGCC 
CCTGAGCTCC GGCCTTCCAC CTCCACAGAC CGACCTGTCA CCCCCAAGCC CACATCTCGG 
ACCACTAGGA GCAGGACAAA TATGTCCTCT GTCAAGACCC CTGAAACAGT TGTCCCCACA 
GCCCCTGAGC TCCAGATTTC CACCTCCACA GACCAACCTG TCACCCCTAA GCCCACATCT 
CGGACCACTA GGAGCAGGAC AAATATGTCC TCTGTGAAGA ACCCTGAATC AACTGTCCCT 
ATAGCCCCTG AGCTCCCACC TTCCACCTCC ACAGAGCAGC CTGTCACCCC TGAGCCCACA 
TCTCGGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGGCA AGACCCCTGA AACACTTGTC 
CCCACAGCCC CTAAGCTCGA GCCTTCCACT TCCACAGACC AACCTGTCAC TCCTGAGCCC 
ACATCTCAGG CCACCAGGGG CAGGACAAAT AGGTCCTCTG TGAAGACCCC TGAAACAGTT 
GTCCCCACAG CCCCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCCTGT TACCCCTGAG 
CCTACGTCTC AGGCTACTAG GGGAAGAACA GATAGATCCT CTGTCAAGAC TCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTACAGGCT TCCGCCTCCA CAGACCAGCC TGTCACCTCT 
GAGCCCACAT CTCGGACCAC TAGGGGAAGA AAAAATCGGT CCTCTGTCAA GACCCCTGAA 
ACAGTTGTGC CCGCAGCCCC TGAGCTCCAG CCTTCCACCT CCACAGACCA ACCTGTCACC 
CCTGAGCCCA CATCTCGGGC CACTAGGGGC AGGACAAATA GGTCCTCTGT CAAGACCCCT 
GAATCAATTG TCCCTATAGC CCCTGAGCTT CAGCCTTCCA CCTCCAGAAA CCAGCTTGTC 
ACCCCTGAGC CCACATCTCG GGCCACTAGG TGCAGGACAA ATAGGTCCTC TGTCAAGACC 
CCTGAGCCAG TTGTCCCCAC AGCCCCTGAG CCCCATCCTA CCACCTCCAC AGACCAGCCT 
GTCACCCCCA AGCTCACATC TAGGGCCACT AGGAGAAAGA CAAATAGGTC CTCTGTCAAG 
ACTCCCAAAC CAGTTGAACC AGCAGCCTCT GATCTTGAGC CTTTTACCCC CACAGACCAG 
TCCGTCACCC CTGAGGCCAT AGCTCAGGGT GGTCAGAGCA AAACACTGAG GTCTTCCACA 
GTAAGAGCTA TGCCGGTTCC TACCACCCCT GAATTCCAAT CTCCTGTCAC CACAGACCAG 
CCTATTTCCC CTGAGCCTAT TACTCAACCC AGTTGCATCA AGAGGCAGAG AGCCGCTGGG 
AACCCTGGCT CCCTCGCAGC TCCCATTGAC CATAAGCCTT GCTCTGCACC CTTGGAACCT 
AAATCCCAGG CCTCAAGGAA CCAAAGATGG GGAGCAGTGA GAGCAGCTGA ATCCCTTACA 
GCCATTCCTG AGCCTGCCTC TCCCCAGCTT CTTGAGACAC CAATTCATGC CTCCCAGATC 
CAAAAGGTGG AACCAGCAGG TAGATCTAGG TTCACCCCGG AGCTCCAGCC TAAGGCCTCT 
CAAAGCCGCA AGAGGTCTTT AGCTACCATG GATTCACCAC CACATCAAAA ACAGCCCCAA 
AGAGGGGAAG TCTCCCAGAA GACAGTGATT ATCAAGGAAG AGGAAGAAGA TACTGCAGAG 
AAGCCAGGGA AGGAAGAGGA TGTCGTGACT CCAAAACCAG GCAAGAGAAA GAGAGACCAG 
GCAGAGGAGG AGCCCAACAG AATACCAAGC CGCAGCCTCC GACGGACCAA ACTTAACCAA 
GAATCAACAG CCCCCAAAGT GCTCTTCACA GGAGTGGTGG ATGCTCGGGG AGAGCGGGCT 
GTGCTGGCAC TGGGGGGAAG TCTGGCTGGT TCAGCGGCAG AGGCTTCCCA CCTGGTCACT 
GATCGCATCC GCCGGACAGT CAAGTTCCTG TGTGCCCTGG GGCGGGGAAT CCCCATTCTG 
TCCCTGGACT GGCTGCATCA GTCCCGCAAG GCTGGTTTCT TCTTACCCCC GGATGAATAT 
GTGGTGACCG ACCCTGAGCA AGAGAAGAAC TTTGGCTTTA GCCTTCAAGA CGCACTGAGC 
AGGGCTCGGG AGCGAAGGCT GCTAGAGGGC TATGAGATCT ATGTGACCCC TGGAGTCCAG 
CCACCACCAC CTCAGATGGG AGAGATTATT AGCTGCTGTG GAGGCACATA CCTACCCAGC 
ATGCCTCGGT CCTATAAGCC TCAGAGAGTT GTGATCACAT GCCCTCAGGA CTTCCCTCAT 
TGCTCCATTC CACTACGGGT TGGGCTGCCC CTCCTCTCGC CTGAGTTCCT GCTGACTGGA 
GTGCTGAAGC AGGAAGCCAA GCCAGAGGCC TTTGTCCTCT CCCCTTTGGA GATGTCATCC 
ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011522.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867011.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.

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CloneID	OHu96934
Clone ID Related Accession (Same CDS sequence)	XM_017011522.2 , XM_017011522.1
Accession Version	XM_017011522.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGCCTCCTGA ATTGCAAGAT GCCACCTGCT GAGAAGGCTT CCAGGATCAG AGCTGCTGAG 
AAGGTTTCCA GGGGCGATCA GGAATCTCCA GATGCTTGTC TGCCTCCTAC AGTACCTGAA 
GCCCCAGCCC CACCCCAAAA GCCCCTTAAC TCTCAGAGCC AGAAACATCT TGCACCTCCG 
CCCCTTCTTT CTCCCCTTTT ACCTTCTATC AAGCCAACCG TTCGTAAGAC CAGGCAAGAT 
GGGAGTCAGG AAGCTCCAGA GGCTCCCTTG TCCTCAGAGC TGGAGCCTTT CCACCCAAAG 
CCTAAAATTA GAACTCGGAA GTCCTCCAGA ATGACACCCT TTCCAGCTAC CTCTGCTGCC 
CCTGAGCCCC ACCCTTCCAC CTCCACAGCC CAGCCAGTCA CTCCCAAGCC CACATCTCAG 
GCCACTAGGA GCAGGACAAA TAGGTCCTCT GTCAAGACCC CTGAACCAGT TGTCCCCACA 
GCCCCTGAGC TCCAGCCTTC CACCTCCACA GACCAGCCTG TCACCTCTGA GCCCACATCT 
CAGGTTACTA GGGGAAGAAA AAGTAGATCC TCTGTCAAGA CCCCTGAAAC AGTTGTGCCC 
ACAGCCCTTG AGCTCCAGCC TTCCACCTCC ACCGACCGAC CTGTCACCTC TGAACCCACC 
TCTCAGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGTCA AGACCCCTGA ACCAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTCAC TTCTGAGCCC 
ACATATCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTGCCCACAG CCCCTGAGCT CCGGCCTTCC ACCTCCACAG ACCGACCTGT CACCCCCAAG 
CCCACATCTC GGACCACTAG GAGCAGGACA AATATGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGATT TCCACCTCCA CAGACCAACC TGTCACCCCT 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTGAA GAACCCTGAA 
TCAACTGTCC CTATAGCCCC TGAGCTCCCA CCTTCCACCT CCACAGAGCA GCCTGTCACC 
CCTGAGCCCA CATCTCGGGC TACTAGGGGA AGAAAAAATA GATCCTCTGG CAAGACCCCT 
GAAACACTTG TCCCCACAGC CCCTAAGCTC GAGCCTTCCA CTTCCACAGA CCAACCTGTC 
ACTCCTGAGC CCACATCTCA GGCCACCAGG GGCAGGACAA ATAGGTCCTC TGTGAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGCCTT CCACCTCCAC AGACCAGCCT 
GTTACCCCTG AGCCTACGTC TCAGGCTACT AGGGGAAGAA CAGATAGATC CTCTGTCAAG 
ACTCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTACAGG CTTCCGCCTC CACAGACCAG 
CCTGTCACCT CTGAGCCCAC ATCTCGGACC ACTAGGGGAA GAAAAAATCG GTCCTCTGTC 
AAGACCCCTG AAACAGTTGT GCCCGCAGCC CCTGAGCTCC AGCCTTCCAC CTCCACAGAC 
CAACCTGTCA CCCCTGAGCC CACATCTCGG GCCACTAGGG GCAGGACAAA TAGGTCCTCT 
GTCAAGACCC CTGAATCAAT TGTCCCTATA GCCCCTGAGC TTCAGCCTTC CACCTCCAGA 
AACCAGCTTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGTGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAGCC AGTTGTCCCC ACAGCCCCTG AGCCCCATCC TACCACCTCC 
ACAGACCAGC CTGTCACCCC CAAGCTCACA TCTAGGGCCA CTAGGAGAAA GACAAATAGG 
TCCTCTGTCA AGACTCCCAA ACCAGTTGAA CCAGCAGCCT CTGATCTTGA GCCTTTTACC 
CCCACAGACC AGTCCGTCAC CCCTGAGGCC ATAGCTCAGG GTGGTCAGAG CAAAACACTG 
AGGTCTTCCA CAGTAAGAGC TATGCCGGTT CCTACCACCC CTGAATTCCA ATCTCCTGTC 
ACCACAGACC AGCCTATTTC CCCTGAGCCT ATTACTCAAC CCAGTTGCAT CAAGAGGCAG 
AGAGCCGCTG GGAACCCTGG CTCCCTCGCA GCTCCCATTG ACCATAAGCC TTGCTCTGCA 
CCCTTGGAAC CTAAATCCCA GGCCTCAAGG AACCAAAGAT GGGGAGCAGT GAGAGCAGCT 
GAATCCCTTA CAGCCATTCC TGAGCCTGCC TCTCCCCAGC TTCTTGAGAC ACCAATTCAT 
GCCTCCCAGA TCCAAAAGGT GGAACCAGCA GGTAGATCTA GGTTCACCCC GGAGCTCCAG 
CCTAAGGCCT CTCAAAGCCG CAAGAGGTCT TTAGCTACCA TGGATTCACC ACCACATCAA 
AAACAGCCCC AAAGAGGGGA AGTCTCCCAG AAGACAGTGA TTATCAAGGA AGAGGAAGAA 
GATACTGCAG AGAAGCCAGG GAAGGAAGAG GATGTCGTGA CTCCAAAACC AGGCAAGAGA 
AAGAGAGACC AGGCAGAGGA GGAGCCCAAC AGAATACCAA GCCGCAGCCT CCGACGGACC 
AAACTTAACC AAGAATCAAC AGCCCCCAAA GTGCTCTTCA CAGGAGTGGT GGATGCTCGG 
GGAGAGCGGG CTGTGCTGGC ACTGGGGGGA AGTCTGGCTG GTTCAGCGGC AGAGGCTTCC 
CACCTGGTCA CTGATCGCAT CCGCCGGACA GTCAAGTTCC TGTGTGCCCT GGGGCGGGGA 
ATCCCCATTC TGTCCCTGGA CTGGCTGCAT CAGTCCCGCA AGGCTGGTTT CTTCTTACCC 
CCGGATGAAT ATGTGGTGAC CGACCCTGAG CAAGAGAAGA ACTTTGGCTT TAGCCTTCAA 
GACGCACTGA GCAGGGCTCG GGAGCGAAGG CTGCTAGAGG GCTATGAGAT CTATGTGACC 
CCTGGAGTCC AGCCACCACC ACCTCAGATG GGAGAGATTA TTAGCTGCTG TGGAGGCACA 
TACCTACCCA GCATGCCTCG GTCCTATAAG CCTCAGAGAG TTGTGATCAC ATGCCCTCAG 
GACTTCCCTC ATTGCTCCAT TCCACTACGG GTTGGGCTGC CCCTCCTCTC GCCTGAGTTC 
CTGCTGACTG GAGTGCTGAA GCAGGAAGCC AAGCCAGAGG CCTTTGTCCT CTCCCCTTTG 
GAGATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867011.1
CDS	282..5819
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011522.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011522.2

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGCCTCCTGA ATTGCAAGAT GCCACCTGCT GAGAAGGCTT CCAGGATCAG AGCTGCTGAG 
AAGGTTTCCA GGGGCGATCA GGAATCTCCA GATGCTTGTC TGCCTCCTAC AGTACCTGAA 
GCCCCAGCCC CACCCCAAAA GCCCCTTAAC TCTCAGAGCC AGAAACATCT TGCACCTCCG 
CCCCTTCTTT CTCCCCTTTT ACCTTCTATC AAGCCAACCG TTCGTAAGAC CAGGCAAGAT 
GGGAGTCAGG AAGCTCCAGA GGCTCCCTTG TCCTCAGAGC TGGAGCCTTT CCACCCAAAG 
CCTAAAATTA GAACTCGGAA GTCCTCCAGA ATGACACCCT TTCCAGCTAC CTCTGCTGCC 
CCTGAGCCCC ACCCTTCCAC CTCCACAGCC CAGCCAGTCA CTCCCAAGCC CACATCTCAG 
GCCACTAGGA GCAGGACAAA TAGGTCCTCT GTCAAGACCC CTGAACCAGT TGTCCCCACA 
GCCCCTGAGC TCCAGCCTTC CACCTCCACA GACCAGCCTG TCACCTCTGA GCCCACATCT 
CAGGTTACTA GGGGAAGAAA AAGTAGATCC TCTGTCAAGA CCCCTGAAAC AGTTGTGCCC 
ACAGCCCTTG AGCTCCAGCC TTCCACCTCC ACCGACCGAC CTGTCACCTC TGAACCCACC 
TCTCAGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGTCA AGACCCCTGA ACCAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTCAC TTCTGAGCCC 
ACATATCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTGCCCACAG CCCCTGAGCT CCGGCCTTCC ACCTCCACAG ACCGACCTGT CACCCCCAAG 
CCCACATCTC GGACCACTAG GAGCAGGACA AATATGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGATT TCCACCTCCA CAGACCAACC TGTCACCCCT 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTGAA GAACCCTGAA 
TCAACTGTCC CTATAGCCCC TGAGCTCCCA CCTTCCACCT CCACAGAGCA GCCTGTCACC 
CCTGAGCCCA CATCTCGGGC TACTAGGGGA AGAAAAAATA GATCCTCTGG CAAGACCCCT 
GAAACACTTG TCCCCACAGC CCCTAAGCTC GAGCCTTCCA CTTCCACAGA CCAACCTGTC 
ACTCCTGAGC CCACATCTCA GGCCACCAGG GGCAGGACAA ATAGGTCCTC TGTGAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGCCTT CCACCTCCAC AGACCAGCCT 
GTTACCCCTG AGCCTACGTC TCAGGCTACT AGGGGAAGAA CAGATAGATC CTCTGTCAAG 
ACTCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTACAGG CTTCCGCCTC CACAGACCAG 
CCTGTCACCT CTGAGCCCAC ATCTCGGACC ACTAGGGGAA GAAAAAATCG GTCCTCTGTC 
AAGACCCCTG AAACAGTTGT GCCCGCAGCC CCTGAGCTCC AGCCTTCCAC CTCCACAGAC 
CAACCTGTCA CCCCTGAGCC CACATCTCGG GCCACTAGGG GCAGGACAAA TAGGTCCTCT 
GTCAAGACCC CTGAATCAAT TGTCCCTATA GCCCCTGAGC TTCAGCCTTC CACCTCCAGA 
AACCAGCTTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGTGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAGCC AGTTGTCCCC ACAGCCCCTG AGCCCCATCC TACCACCTCC 
ACAGACCAGC CTGTCACCCC CAAGCTCACA TCTAGGGCCA CTAGGAGAAA GACAAATAGG 
TCCTCTGTCA AGACTCCCAA ACCAGTTGAA CCAGCAGCCT CTGATCTTGA GCCTTTTACC 
CCCACAGACC AGTCCGTCAC CCCTGAGGCC ATAGCTCAGG GTGGTCAGAG CAAAACACTG 
AGGTCTTCCA CAGTAAGAGC TATGCCGGTT CCTACCACCC CTGAATTCCA ATCTCCTGTC 
ACCACAGACC AGCCTATTTC CCCTGAGCCT ATTACTCAAC CCAGTTGCAT CAAGAGGCAG 
AGAGCCGCTG GGAACCCTGG CTCCCTCGCA GCTCCCATTG ACCATAAGCC TTGCTCTGCA 
CCCTTGGAAC CTAAATCCCA GGCCTCAAGG AACCAAAGAT GGGGAGCAGT GAGAGCAGCT 
GAATCCCTTA CAGCCATTCC TGAGCCTGCC TCTCCCCAGC TTCTTGAGAC ACCAATTCAT 
GCCTCCCAGA TCCAAAAGGT GGAACCAGCA GGTAGATCTA GGTTCACCCC GGAGCTCCAG 
CCTAAGGCCT CTCAAAGCCG CAAGAGGTCT TTAGCTACCA TGGATTCACC ACCACATCAA 
AAACAGCCCC AAAGAGGGGA AGTCTCCCAG AAGACAGTGA TTATCAAGGA AGAGGAAGAA 
GATACTGCAG AGAAGCCAGG GAAGGAAGAG GATGTCGTGA CTCCAAAACC AGGCAAGAGA 
AAGAGAGACC AGGCAGAGGA GGAGCCCAAC AGAATACCAA GCCGCAGCCT CCGACGGACC 
AAACTTAACC AAGAATCAAC AGCCCCCAAA GTGCTCTTCA CAGGAGTGGT GGATGCTCGG 
GGAGAGCGGG CTGTGCTGGC ACTGGGGGGA AGTCTGGCTG GTTCAGCGGC AGAGGCTTCC 
CACCTGGTCA CTGATCGCAT CCGCCGGACA GTCAAGTTCC TGTGTGCCCT GGGGCGGGGA 
ATCCCCATTC TGTCCCTGGA CTGGCTGCAT CAGTCCCGCA AGGCTGGTTT CTTCTTACCC 
CCGGATGAAT ATGTGGTGAC CGACCCTGAG CAAGAGAAGA ACTTTGGCTT TAGCCTTCAA 
GACGCACTGA GCAGGGCTCG GGAGCGAAGG CTGCTAGAGG GCTATGAGAT CTATGTGACC 
CCTGGAGTCC AGCCACCACC ACCTCAGATG GGAGAGATTA TTAGCTGCTG TGGAGGCACA 
TACCTACCCA GCATGCCTCG GTCCTATAAG CCTCAGAGAG TTGTGATCAC ATGCCCTCAG 
GACTTCCCTC ATTGCTCCAT TCCACTACGG GTTGGGCTGC CCCTCCTCTC GCCTGAGTTC 
CTGCTGACTG GAGTGCTGAA GCAGGAAGCC AAGCCAGAGG CCTTTGTCCT CTCCCCTTTG 
GAGATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_017011522.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016867011.1 Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.

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CloneID	OHu96934
Clone ID Related Accession (Same CDS sequence)	XM_017011522.2 , XM_017011522.1
Accession Version	XM_017011522.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of DNA damage checkpoint protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGCCTCCTGA ATTGCAAGAT GCCACCTGCT GAGAAGGCTT CCAGGATCAG AGCTGCTGAG 
AAGGTTTCCA GGGGCGATCA GGAATCTCCA GATGCTTGTC TGCCTCCTAC AGTACCTGAA 
GCCCCAGCCC CACCCCAAAA GCCCCTTAAC TCTCAGAGCC AGAAACATCT TGCACCTCCG 
CCCCTTCTTT CTCCCCTTTT ACCTTCTATC AAGCCAACCG TTCGTAAGAC CAGGCAAGAT 
GGGAGTCAGG AAGCTCCAGA GGCTCCCTTG TCCTCAGAGC TGGAGCCTTT CCACCCAAAG 
CCTAAAATTA GAACTCGGAA GTCCTCCAGA ATGACACCCT TTCCAGCTAC CTCTGCTGCC 
CCTGAGCCCC ACCCTTCCAC CTCCACAGCC CAGCCAGTCA CTCCCAAGCC CACATCTCAG 
GCCACTAGGA GCAGGACAAA TAGGTCCTCT GTCAAGACCC CTGAACCAGT TGTCCCCACA 
GCCCCTGAGC TCCAGCCTTC CACCTCCACA GACCAGCCTG TCACCTCTGA GCCCACATCT 
CAGGTTACTA GGGGAAGAAA AAGTAGATCC TCTGTCAAGA CCCCTGAAAC AGTTGTGCCC 
ACAGCCCTTG AGCTCCAGCC TTCCACCTCC ACCGACCGAC CTGTCACCTC TGAACCCACC 
TCTCAGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGTCA AGACCCCTGA ACCAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTCAC TTCTGAGCCC 
ACATATCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTGCCCACAG CCCCTGAGCT CCGGCCTTCC ACCTCCACAG ACCGACCTGT CACCCCCAAG 
CCCACATCTC GGACCACTAG GAGCAGGACA AATATGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGATT TCCACCTCCA CAGACCAACC TGTCACCCCT 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTGAA GAACCCTGAA 
TCAACTGTCC CTATAGCCCC TGAGCTCCCA CCTTCCACCT CCACAGAGCA GCCTGTCACC 
CCTGAGCCCA CATCTCGGGC TACTAGGGGA AGAAAAAATA GATCCTCTGG CAAGACCCCT 
GAAACACTTG TCCCCACAGC CCCTAAGCTC GAGCCTTCCA CTTCCACAGA CCAACCTGTC 
ACTCCTGAGC CCACATCTCA GGCCACCAGG GGCAGGACAA ATAGGTCCTC TGTGAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGCCTT CCACCTCCAC AGACCAGCCT 
GTTACCCCTG AGCCTACGTC TCAGGCTACT AGGGGAAGAA CAGATAGATC CTCTGTCAAG 
ACTCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTACAGG CTTCCGCCTC CACAGACCAG 
CCTGTCACCT CTGAGCCCAC ATCTCGGACC ACTAGGGGAA GAAAAAATCG GTCCTCTGTC 
AAGACCCCTG AAACAGTTGT GCCCGCAGCC CCTGAGCTCC AGCCTTCCAC CTCCACAGAC 
CAACCTGTCA CCCCTGAGCC CACATCTCGG GCCACTAGGG GCAGGACAAA TAGGTCCTCT 
GTCAAGACCC CTGAATCAAT TGTCCCTATA GCCCCTGAGC TTCAGCCTTC CACCTCCAGA 
AACCAGCTTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGTGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAGCC AGTTGTCCCC ACAGCCCCTG AGCCCCATCC TACCACCTCC 
ACAGACCAGC CTGTCACCCC CAAGCTCACA TCTAGGGCCA CTAGGAGAAA GACAAATAGG 
TCCTCTGTCA AGACTCCCAA ACCAGTTGAA CCAGCAGCCT CTGATCTTGA GCCTTTTACC 
CCCACAGACC AGTCCGTCAC CCCTGAGGCC ATAGCTCAGG GTGGTCAGAG CAAAACACTG 
AGGTCTTCCA CAGTAAGAGC TATGCCGGTT CCTACCACCC CTGAATTCCA ATCTCCTGTC 
ACCACAGACC AGCCTATTTC CCCTGAGCCT ATTACTCAAC CCAGTTGCAT CAAGAGGCAG 
AGAGCCGCTG GGAACCCTGG CTCCCTCGCA GCTCCCATTG ACCATAAGCC TTGCTCTGCA 
CCCTTGGAAC CTAAATCCCA GGCCTCAAGG AACCAAAGAT GGGGAGCAGT GAGAGCAGCT 
GAATCCCTTA CAGCCATTCC TGAGCCTGCC TCTCCCCAGC TTCTTGAGAC ACCAATTCAT 
GCCTCCCAGA TCCAAAAGGT GGAACCAGCA GGTAGATCTA GGTTCACCCC GGAGCTCCAG 
CCTAAGGCCT CTCAAAGCCG CAAGAGGTCT TTAGCTACCA TGGATTCACC ACCACATCAA 
AAACAGCCCC AAAGAGGGGA AGTCTCCCAG AAGACAGTGA TTATCAAGGA AGAGGAAGAA 
GATACTGCAG AGAAGCCAGG GAAGGAAGAG GATGTCGTGA CTCCAAAACC AGGCAAGAGA 
AAGAGAGACC AGGCAGAGGA GGAGCCCAAC AGAATACCAA GCCGCAGCCT CCGACGGACC 
AAACTTAACC AAGAATCAAC AGCCCCCAAA GTGCTCTTCA CAGGAGTGGT GGATGCTCGG 
GGAGAGCGGG CTGTGCTGGC ACTGGGGGGA AGTCTGGCTG GTTCAGCGGC AGAGGCTTCC 
CACCTGGTCA CTGATCGCAT CCGCCGGACA GTCAAGTTCC TGTGTGCCCT GGGGCGGGGA 
ATCCCCATTC TGTCCCTGGA CTGGCTGCAT CAGTCCCGCA AGGCTGGTTT CTTCTTACCC 
CCGGATGAAT ATGTGGTGAC CGACCCTGAG CAAGAGAAGA ACTTTGGCTT TAGCCTTCAA 
GACGCACTGA GCAGGGCTCG GGAGCGAAGG CTGCTAGAGG GCTATGAGAT CTATGTGACC 
CCTGGAGTCC AGCCACCACC ACCTCAGATG GGAGAGATTA TTAGCTGCTG TGGAGGCACA 
TACCTACCCA GCATGCCTCG GTCCTATAAG CCTCAGAGAG TTGTGATCAC ATGCCCTCAG 
GACTTCCCTC ATTGCTCCAT TCCACTACGG GTTGGGCTGC CCCTCCTCTC GCCTGAGTTC 
CTGCTGACTG GAGTGCTGAA GCAGGAAGCC AAGCCAGAGG CCTTTGTCCT CTCCCCTTTG 
GAGATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016867011.1
CDS	282..5819
Translation	MGHPTFPTCQQFPSRVAYFQIMEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST

Target ORF information:

RefSeq Version	XM_017011522.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator of DNA damage checkpoint 1 (MDC1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017011522.1

ORF Insert Sequence:

ATGGGGCATC CTACTTTCCC TACCTGTCAA CAGTTTCCCT CTAGAGTGGC ATATTTTCAG 
ATCATGGAGG ACACCCAGGC TATTGACTGG GATGTTGAAG AAGAGGAGGA GACAGAGCAA 
TCCAGTGAAT CCTTGAGGTG TAACGTGGAG CCAGTAGGGC GGCTACATAT CTTTAGTGGT 
GCCCATGGAC CAGAAAAAGA TTTCCCACTA CACCTCGGGA AGAATGTGGT AGGCCGAATG 
CCTGACTGCT CTGTGGCCCT GCCCTTTCCA TCTATCTCCA AACAACATGC AGAGATTGAA 
ATCTTAGCCT GGGACAAGGC ACCTATCCTC CGAGACTGTG GGAGCCTTAA TGGTACTCAA 
ATCCTGAGAC CTCCTAAGGT TTTGAGCCCT GGGGTGAGTC ACCGTCTGAG GGACCAGGAA 
TTGATTCTCT TTGCTGACTT GCTCTGCCAG TACCATCGCC TGGATGTCTC TCTGCCCTTT 
GTCTCCCGGG GCCCTCTGAC AGTAGAAGAG ACACCCAGAG TACAGGGAGA AACTCAACCC 
CAGAGGCTTC TGTTGGCTGA GGACTCGGAG GAGGAAGTAG ATTTTCTTTC TGAAAGGCGT 
ATGGTAAAAA AATCAAGGAC CACATCTTCC TCTGTGATAG TTCCAGAGAG TGATGAAGAG 
GGGCATTCCC CGGTCCTGGG CGGCCTTGGG CCGCCTTTTG CCTTCAATTT GAACAGTGAC 
ACAGATGTGG AAGAAGGTCA GCAACCAGCC ACAGAGGAGG CCTCCTCAGC TGCCAGAAGA 
GGTGCCACTG TAGAGGCAAA GCAGTCTGAA GCTGAAGTTG TAACTGAAAT CCAGCTTGAA 
AAGGATCAGC CTTTAGTGAA GGAGAGGGAC AATGATACAA AAGTCAAGAG GGGTGCAGGG 
AATGGGGTGG TTCCAGCTGG GGTGATTCTG GAGAGGAGCC AACCTCCTGG AGAGGACAGT 
GACACAGATG TGGATGATGA CAGCAGGCCT CCTGGAAGGC CAGCTGAGGT CCATTTGGAA 
AGGGCTCAGC CTTTTGGCTT CATCGACAGC GACACTGATG CGGAAGAAGA GAGGATCCCA 
GCAACCCCAG TTGTCATTCC TATGAAGAAG AGGAAGATCT TCCATGGAGT AGGTACAAGG 
GGTCCTGGAG CACCAGGCCT GGCCCATCTG CAGGAGAGCC AGGCTGGTAG TGATACAGAT 
GTGGAAGAAG GCAAGGCCCC ACAGGCTGTC CCTCTGGAGA AAAGCCAAGC TTCCATGGTT 
ATCAACAGCG ATACAGATGA CGAGGAAGAA GTCTCAGCAG CGCTGACTTT GGCACATCTG 
AAAGAGAGCC AGCCTGCTAT ATGGAACAGA GATGCAGAAG AGGACATGCC CCAACGTGTG 
GTCCTTCTGC AGCGAAGCCA AACCACCACT GAGAGAGACA GTGACACAGA CGTGGAGGAG 
GAAGAGCTCC CAGTGGAAAA TAGAGAAGCT GTCCTCAAGG ATCACACAAA GATTAGAGCC 
CTTGTTAGAG CACATTCAGA AAAGGACCAA CCTCCTTTTG GGGACAGTGA TGACAGTGTG 
GAAGCAGATA AGAGCTCACC TGGGATCCAC CTGGAGAGAA GCCAAGCCTC CACCACAGTG 
GACATCAACA CACAAGTGGA GAAGGAAGTC CCGCCAGGGT CAGCCATTAT ACATATAAAG 
AAGCATCAGG TGTCTGTGGA GGGGACAAAT CAAACAGATG TGAAAGCAGT TGGGGGACCA 
GCAAAGCTGC TTGTGGTATC TCTAGAGGAA GCCTGGCCTC TGCATGGGGA CTGTGAAACA 
GATGCAGAGG AGGGCACCTC CCTAACAGCC TCAGTAGTTG CAGATGTAAG AAAGAGCCAG 
CTTCCAGCAG AAGGGGATGC TGGGGCAGAG TGGGCTGCAG CTGTTCTTAA GCAGGAGAGA 
GCTCATGAGG TGGGGGCCCA GGGTGGGCCA CCTGTGGCAC AAGTGGAGCA GGACCTCCCT 
ATCTCAAGAG AGAACCTCAC AGATCTGGTG GTGGACACAG ACACTCTAGG GGAATCCACC 
CAGCCACAGA GAGAGGGAGC CCAGGTCCCC ACAGGAAGGG AGAGAGAACA ACATGTGGGT 
GGGACCAAGG ACTCTGAAGA CAACTATGGT GATTCTGAAG ATCTGGACCT ACAAGCTACC 
CAGTGCTTTC TGGAGAATCA GGGCCTGGAA GTCCAGAGCA TGGAGGATGA ACCTACCCAG 
GCCTTCATGT TGACTCCACC CCAAGAGCTT GGCCCTTCCC ATTGCAGCTT CCAGACAACA 
GGCCTCCTGA ATTGCAAGAT GCCACCTGCT GAGAAGGCTT CCAGGATCAG AGCTGCTGAG 
AAGGTTTCCA GGGGCGATCA GGAATCTCCA GATGCTTGTC TGCCTCCTAC AGTACCTGAA 
GCCCCAGCCC CACCCCAAAA GCCCCTTAAC TCTCAGAGCC AGAAACATCT TGCACCTCCG 
CCCCTTCTTT CTCCCCTTTT ACCTTCTATC AAGCCAACCG TTCGTAAGAC CAGGCAAGAT 
GGGAGTCAGG AAGCTCCAGA GGCTCCCTTG TCCTCAGAGC TGGAGCCTTT CCACCCAAAG 
CCTAAAATTA GAACTCGGAA GTCCTCCAGA ATGACACCCT TTCCAGCTAC CTCTGCTGCC 
CCTGAGCCCC ACCCTTCCAC CTCCACAGCC CAGCCAGTCA CTCCCAAGCC CACATCTCAG 
GCCACTAGGA GCAGGACAAA TAGGTCCTCT GTCAAGACCC CTGAACCAGT TGTCCCCACA 
GCCCCTGAGC TCCAGCCTTC CACCTCCACA GACCAGCCTG TCACCTCTGA GCCCACATCT 
CAGGTTACTA GGGGAAGAAA AAGTAGATCC TCTGTCAAGA CCCCTGAAAC AGTTGTGCCC 
ACAGCCCTTG AGCTCCAGCC TTCCACCTCC ACCGACCGAC CTGTCACCTC TGAACCCACC 
TCTCAGGCTA CTAGGGGAAG AAAAAATAGA TCCTCTGTCA AGACCCCTGA ACCAGTTGTC 
CCCACAGCCC CTGAGCTCCA GCCTTCCACC TCCACAGACC AGCCTGTCAC TTCTGAGCCC 
ACATATCAGG CTACTAGGGG AAGAAAAAAT AGATCCTCTG TCAAGACCCC TGAACCAGTT 
GTGCCCACAG CCCCTGAGCT CCGGCCTTCC ACCTCCACAG ACCGACCTGT CACCCCCAAG 
CCCACATCTC GGACCACTAG GAGCAGGACA AATATGTCCT CTGTCAAGAC CCCTGAAACA 
GTTGTCCCCA CAGCCCCTGA GCTCCAGATT TCCACCTCCA CAGACCAACC TGTCACCCCT 
AAGCCCACAT CTCGGACCAC TAGGAGCAGG ACAAATATGT CCTCTGTGAA GAACCCTGAA 
TCAACTGTCC CTATAGCCCC TGAGCTCCCA CCTTCCACCT CCACAGAGCA GCCTGTCACC 
CCTGAGCCCA CATCTCGGGC TACTAGGGGA AGAAAAAATA GATCCTCTGG CAAGACCCCT 
GAAACACTTG TCCCCACAGC CCCTAAGCTC GAGCCTTCCA CTTCCACAGA CCAACCTGTC 
ACTCCTGAGC CCACATCTCA GGCCACCAGG GGCAGGACAA ATAGGTCCTC TGTGAAGACC 
CCTGAAACAG TTGTCCCCAC AGCCCCTGAG CTCCAGCCTT CCACCTCCAC AGACCAGCCT 
GTTACCCCTG AGCCTACGTC TCAGGCTACT AGGGGAAGAA CAGATAGATC CTCTGTCAAG 
ACTCCTGAAA CAGTTGTCCC CACAGCCCCT GAGCTACAGG CTTCCGCCTC CACAGACCAG 
CCTGTCACCT CTGAGCCCAC ATCTCGGACC ACTAGGGGAA GAAAAAATCG GTCCTCTGTC 
AAGACCCCTG AAACAGTTGT GCCCGCAGCC CCTGAGCTCC AGCCTTCCAC CTCCACAGAC 
CAACCTGTCA CCCCTGAGCC CACATCTCGG GCCACTAGGG GCAGGACAAA TAGGTCCTCT 
GTCAAGACCC CTGAATCAAT TGTCCCTATA GCCCCTGAGC TTCAGCCTTC CACCTCCAGA 
AACCAGCTTG TCACCCCTGA GCCCACATCT CGGGCCACTA GGTGCAGGAC AAATAGGTCC 
TCTGTCAAGA CCCCTGAGCC AGTTGTCCCC ACAGCCCCTG AGCCCCATCC TACCACCTCC 
ACAGACCAGC CTGTCACCCC CAAGCTCACA TCTAGGGCCA CTAGGAGAAA GACAAATAGG 
TCCTCTGTCA AGACTCCCAA ACCAGTTGAA CCAGCAGCCT CTGATCTTGA GCCTTTTACC 
CCCACAGACC AGTCCGTCAC CCCTGAGGCC ATAGCTCAGG GTGGTCAGAG CAAAACACTG 
AGGTCTTCCA CAGTAAGAGC TATGCCGGTT CCTACCACCC CTGAATTCCA ATCTCCTGTC 
ACCACAGACC AGCCTATTTC CCCTGAGCCT ATTACTCAAC CCAGTTGCAT CAAGAGGCAG 
AGAGCCGCTG GGAACCCTGG CTCCCTCGCA GCTCCCATTG ACCATAAGCC TTGCTCTGCA 
CCCTTGGAAC CTAAATCCCA GGCCTCAAGG AACCAAAGAT GGGGAGCAGT GAGAGCAGCT 
GAATCCCTTA CAGCCATTCC TGAGCCTGCC TCTCCCCAGC TTCTTGAGAC ACCAATTCAT 
GCCTCCCAGA TCCAAAAGGT GGAACCAGCA GGTAGATCTA GGTTCACCCC GGAGCTCCAG 
CCTAAGGCCT CTCAAAGCCG CAAGAGGTCT TTAGCTACCA TGGATTCACC ACCACATCAA 
AAACAGCCCC AAAGAGGGGA AGTCTCCCAG AAGACAGTGA TTATCAAGGA AGAGGAAGAA 
GATACTGCAG AGAAGCCAGG GAAGGAAGAG GATGTCGTGA CTCCAAAACC AGGCAAGAGA 
AAGAGAGACC AGGCAGAGGA GGAGCCCAAC AGAATACCAA GCCGCAGCCT CCGACGGACC 
AAACTTAACC AAGAATCAAC AGCCCCCAAA GTGCTCTTCA CAGGAGTGGT GGATGCTCGG 
GGAGAGCGGG CTGTGCTGGC ACTGGGGGGA AGTCTGGCTG GTTCAGCGGC AGAGGCTTCC 
CACCTGGTCA CTGATCGCAT CCGCCGGACA GTCAAGTTCC TGTGTGCCCT GGGGCGGGGA 
ATCCCCATTC TGTCCCTGGA CTGGCTGCAT CAGTCCCGCA AGGCTGGTTT CTTCTTACCC 
CCGGATGAAT ATGTGGTGAC CGACCCTGAG CAAGAGAAGA ACTTTGGCTT TAGCCTTCAA 
GACGCACTGA GCAGGGCTCG GGAGCGAAGG CTGCTAGAGG GCTATGAGAT CTATGTGACC 
CCTGGAGTCC AGCCACCACC ACCTCAGATG GGAGAGATTA TTAGCTGCTG TGGAGGCACA 
TACCTACCCA GCATGCCTCG GTCCTATAAG CCTCAGAGAG TTGTGATCAC ATGCCCTCAG 
GACTTCCCTC ATTGCTCCAT TCCACTACGG GTTGGGCTGC CCCTCCTCTC GCCTGAGTTC 
CTGCTGACTG GAGTGCTGAA GCAGGAAGCC AAGCCAGAGG CCTTTGTCCT CTCCCCTTTG 
GAGATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SOX9 activity is induced by oncogenic Kras to affect MDC1 and MCMs expression in pancreatic cancer.
Oncogene(2017 Oct)
Zhou H,Qin Y,Ji S,Ling J,Fu J,Zhuang Z,Fan X,Song L,Yu X,Chiao PJ

見積依頼

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オンライン注文

MDC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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