IKBKE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IKBKE
Entrez Gene ID	9641
Full Name	inhibitor of nuclear factor kappa B kinase subunit epsilon
Synonyms	IKK-E,IKK-i,IKKE,IKKI
General protein information	Preferred Names inhibitor of nuclear factor kappa B kinase subunit epsilon Names inhibitor of nuclear factor kappa-B kinase subunit epsilon I-kappa-B kinase epsilon IKK-epsilon IKK-related kinase epsilon inducible I kappa-B kinase inducible IkappaB kinase inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009].
Disorder MIM:	605048

mRNA and Protein(s)

mRNA	Protein	Name
NM_001193322.1	NP_001180251.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 2
NM_001193321.2	NP_001180250.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 3
XM_005273356.2	XP_005273413.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform X1
NM_001193321.1	NP_001180250.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 3
NM_001193322.2	NP_001180251.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 2
XM_017002868.1	XP_016858357.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform X2
NM_014002.3	NP_054721.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 1
NM_014002.4	NP_054721.1	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 1

Pathways from BioSystems

Homologies

	IKBKE	XP_001088252.1
Rattus norvegicus (Norway rat)	Ikbke	NP_001102324.1
Homo sapiens (human)	IKBKE	NP_054721.1
Xenopus tropicalis (tropical clawed frog)	ikbke	XP_002940557.2
Canis lupus familiaris (dog)	IKBKE	XP_536108.3
Mus musculus (house mouse)	Ikbke	NP_062751.2
Danio rerio (zebrafish)	ikbke	NP_001002751.1
Bos taurus (cattle)	IKBKE	NP_001039810.1
Gallus gallus (chicken)	IKBKE	XP_004935017.1

Gene Ontology

The following IKBKE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IKBKE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27210	NM_001193321.2 Latest version!	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu26692	NM_001193322.2 Latest version!	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu90822	XM_017002868.1 Latest version!	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu26712	NM_014002.4 Latest version!	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu26712	XM_005273356.2 Latest version!	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu27210	NM_001193321.1	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu26692	NM_001193322.1	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu26712	NM_014002.3	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IKBKE ( NM_001193321.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180250.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27210
Clone ID Related Accession (Same CDS sequence)	NM_001193321.2 , NM_001193321.1
Accession Version	NM_001193321.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA999897.1, BC107812.1, D63485.1 and CB243308.1. On May 31, 2019 this sequence version replaced NM_001193321.1. Transcript Variant: This variant (3) lacks an alternate segment which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTACT GCTCCAGTGG GAGCCTGCTG AGTGTGCTGG AGAGCCCTGA GAATGCCTTT 
GGGCTGCCTG AGGATGAGTT CCTGGTGGTG CTGCGCTGTG TGGTGGCCGG CATGAACCAC 
CTGCGGGAGA ACGGCATTGT GCATCGCGAC ATCAAGCCGG GGAACATCAT GCGCCTCGTA 
GGGGAGGAGG GGCAGAGCAT CTACAAGCTG ACAGACTTCG GCGCTGCCCG GGAGCTGGAT 
GATGATGAGA AGTTCGTCTC GGTCTATGGG ACTGAGGAGT ACCTGCATCC CGACATGTAT 
GAGCGGGCGG TGCTTCGAAA GCCCCAGCAA AAAGCGTTCG GGGTGACTGT GGATCTCTGG 
AGCATTGGAG TGACCTTGTA CCATGCAGCC ACTGGCAGCC TGCCCTTCAT CCCCTTTGGT 
GGGCCACGGC GGAACAAGGA GATCATGTAC CGGATCACCA CGGAGAAGCC GGCTGGGGCC 
ATTGCAGGTG CCCAGAGGCG GGAGAACGGG CCCCTGGAGT GGAGCTACAC CCTCCCCATC 
ACCTGCCAGC TGTCACTGGG GCTGCAGAGC CAGCTGGTGC CCATCCTGGC CAACATCCTG 
GAGGTGGAGC AGGCCAAGTG CTGGGGCTTC GACCAGTTCT TTGCGGAGAC CAGTGACATC 
CTGCAGCGAG TTGTCGTCCA TGTCTTCTCC CTGTCCCAGG CAGTCCTGCA CCACATCTAT 
ATCCATGCCC ACAACACGAT AGCCATTTTC CAGGAGGCCG TGCACAAGCA GACCAGTGTG 
GCCCCCCGAC ACCAGGAGTA CCTCTTTGAG GGTCACCTCT GTGTCCTCGA GCCCAGCGTC 
TCAGCACAGC ACATCGCCCA CACGACGGCA AGCAGCCCCC TGACCCTCTT CAGCACAGCC 
ATCCCTAAGG GGCTGGCCTT CAGGGACCCT GCTCTGGACG TCCCCAAGTT CGTCCCCAAA 
GTGGACCTGC AGGCGGATTA CAACACTGCC AAGGGCGTGT TGGGCGCCGG CTACCAGGCC 
CTGCGGCTGG CACGGGCCCT GCTGGATGGG CAGGAGCTAA TGTTTCGGGG GCTGCACTGG 
GTCATGGAGG TGCTCCAGGC CACATGCAGA CGGACTCTGG AAGTGGCAAG GACATCCCTC 
CTCTACCTCA GCAGCAGCCT GGGAACTGAG AGGTTCAGCA GCGTGGCTGG AACGCCTGAG 
ATCCAGGAAC TGAAGGCGGC TGCAGAACTG AGGTCCAGGC TGCGGACTCT AGCGGAGGTC 
CTCTCCAGAT GCTCCCAAAA TATCACGGAG ACCCAGGAGA GCCTGAGCAG CCTGAACCGG 
GAGCTGGTGA AGAGCCGGGA TCAGGTACAT GAGGACAGAA GCATCCAGCA GATTCAGTGC 
TGTTTGGACA AGATGAACTT CATCTACAAA CAGTTCAAGA AGTCTAGGAT GAGGCCAGGG 
CTTGGCTACA ACGAGGAGCA GATTCACAAG CTGGATAAGG TGAATTTCAG TCATTTAGCC 
AAAAGACTCC TGCAGGTGTT CCAGGAGGAG TGCGTGCAGA AGTATCAAGC GTCCTTAGTC 
ACACACGGCA AGAGGATGAG GGTGGTGCAC GAGACCAGGA ACCACCTGCG CCTGGTTGGC 
TGTTCTGTGG CTGCCTGTAA CACAGAAGCC CAGGGGGTCC AGGAGAGTCT CAGCAAGCTC 
CTGGAAGAGC TATCTCACCA GCTCCTTCAG GACCGAGCAA AGGGGGCTCA GGCCTCGCCG 
CCTCCCATAG CTCCTTACCC CAGCCCTACA CGAAAGGACC TGCTTCTCCA CATGCAAGAG 
CTCTGCGAGG GGATGAAGCT GCTGGCATCT GACCTCCTGG ACAACAACCG CATCATCGAA 
CGGCTAAATA GAGTCCCAGC ACCTCCTGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180250.1
CDS	482..2377
Translation	MEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Target ORF information:

RefSeq Version	NM_001193321.2
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193321.2

ORF Insert Sequence:

ATGGAGTACT GCTCCAGTGG GAGCCTGCTG AGTGTGCTGG AGAGCCCTGA GAATGCCTTT 
GGGCTGCCTG AGGATGAGTT CCTGGTGGTG CTGCGCTGTG TGGTGGCCGG CATGAACCAC 
CTGCGGGAGA ACGGCATTGT GCATCGCGAC ATCAAGCCGG GGAACATCAT GCGCCTCGTA 
GGGGAGGAGG GGCAGAGCAT CTACAAGCTG ACAGACTTCG GCGCTGCCCG GGAGCTGGAT 
GATGATGAGA AGTTCGTCTC GGTCTATGGG ACTGAGGAGT ACCTGCATCC CGACATGTAT 
GAGCGGGCGG TGCTTCGAAA GCCCCAGCAA AAAGCGTTCG GGGTGACTGT GGATCTCTGG 
AGCATTGGAG TGACCTTGTA CCATGCAGCC ACTGGCAGCC TGCCCTTCAT CCCCTTTGGT 
GGGCCACGGC GGAACAAGGA GATCATGTAC CGGATCACCA CGGAGAAGCC GGCTGGGGCC 
ATTGCAGGTG CCCAGAGGCG GGAGAACGGG CCCCTGGAGT GGAGCTACAC CCTCCCCATC 
ACCTGCCAGC TGTCACTGGG GCTGCAGAGC CAGCTGGTGC CCATCCTGGC CAACATCCTG 
GAGGTGGAGC AGGCCAAGTG CTGGGGCTTC GACCAGTTCT TTGCGGAGAC CAGTGACATC 
CTGCAGCGAG TTGTCGTCCA TGTCTTCTCC CTGTCCCAGG CAGTCCTGCA CCACATCTAT 
ATCCATGCCC ACAACACGAT AGCCATTTTC CAGGAGGCCG TGCACAAGCA GACCAGTGTG 
GCCCCCCGAC ACCAGGAGTA CCTCTTTGAG GGTCACCTCT GTGTCCTCGA GCCCAGCGTC 
TCAGCACAGC ACATCGCCCA CACGACGGCA AGCAGCCCCC TGACCCTCTT CAGCACAGCC 
ATCCCTAAGG GGCTGGCCTT CAGGGACCCT GCTCTGGACG TCCCCAAGTT CGTCCCCAAA 
GTGGACCTGC AGGCGGATTA CAACACTGCC AAGGGCGTGT TGGGCGCCGG CTACCAGGCC 
CTGCGGCTGG CACGGGCCCT GCTGGATGGG CAGGAGCTAA TGTTTCGGGG GCTGCACTGG 
GTCATGGAGG TGCTCCAGGC CACATGCAGA CGGACTCTGG AAGTGGCAAG GACATCCCTC 
CTCTACCTCA GCAGCAGCCT GGGAACTGAG AGGTTCAGCA GCGTGGCTGG AACGCCTGAG 
ATCCAGGAAC TGAAGGCGGC TGCAGAACTG AGGTCCAGGC TGCGGACTCT AGCGGAGGTC 
CTCTCCAGAT GCTCCCAAAA TATCACGGAG ACCCAGGAGA GCCTGAGCAG CCTGAACCGG 
GAGCTGGTGA AGAGCCGGGA TCAGGTACAT GAGGACAGAA GCATCCAGCA GATTCAGTGC 
TGTTTGGACA AGATGAACTT CATCTACAAA CAGTTCAAGA AGTCTAGGAT GAGGCCAGGG 
CTTGGCTACA ACGAGGAGCA GATTCACAAG CTGGATAAGG TGAATTTCAG TCATTTAGCC 
AAAAGACTCC TGCAGGTGTT CCAGGAGGAG TGCGTGCAGA AGTATCAAGC GTCCTTAGTC 
ACACACGGCA AGAGGATGAG GGTGGTGCAC GAGACCAGGA ACCACCTGCG CCTGGTTGGC 
TGTTCTGTGG CTGCCTGTAA CACAGAAGCC CAGGGGGTCC AGGAGAGTCT CAGCAAGCTC 
CTGGAAGAGC TATCTCACCA GCTCCTTCAG GACCGAGCAA AGGGGGCTCA GGCCTCGCCG 
CCTCCCATAG CTCCTTACCC CAGCCCTACA CGAAAGGACC TGCTTCTCCA CATGCAAGAG 
CTCTGCGAGG GGATGAAGCT GCTGGCATCT GACCTCCTGG ACAACAACCG CATCATCGAA 
CGGCTAAATA GAGTCCCAGC ACCTCCTGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( NM_001193321.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180250.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27210
Clone ID Related Accession (Same CDS sequence)	NM_001193321.2 , NM_001193321.1
Accession Version	NM_001193321.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA999897.1, BC107812.1, D63485.1 and CB243308.1. Transcript Variant: This variant (3) lacks an alternate segment which results in the use of a downstream start codon, compared to variant 1. The resulting protein (isoform 3) is shorter when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC107812.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGAGTACT GCTCCAGTGG GAGCCTGCTG AGTGTGCTGG AGAGCCCTGA GAATGCCTTT 
GGGCTGCCTG AGGATGAGTT CCTGGTGGTG CTGCGCTGTG TGGTGGCCGG CATGAACCAC 
CTGCGGGAGA ACGGCATTGT GCATCGCGAC ATCAAGCCGG GGAACATCAT GCGCCTCGTA 
GGGGAGGAGG GGCAGAGCAT CTACAAGCTG ACAGACTTCG GCGCTGCCCG GGAGCTGGAT 
GATGATGAGA AGTTCGTCTC GGTCTATGGG ACTGAGGAGT ACCTGCATCC CGACATGTAT 
GAGCGGGCGG TGCTTCGAAA GCCCCAGCAA AAAGCGTTCG GGGTGACTGT GGATCTCTGG 
AGCATTGGAG TGACCTTGTA CCATGCAGCC ACTGGCAGCC TGCCCTTCAT CCCCTTTGGT 
GGGCCACGGC GGAACAAGGA GATCATGTAC CGGATCACCA CGGAGAAGCC GGCTGGGGCC 
ATTGCAGGTG CCCAGAGGCG GGAGAACGGG CCCCTGGAGT GGAGCTACAC CCTCCCCATC 
ACCTGCCAGC TGTCACTGGG GCTGCAGAGC CAGCTGGTGC CCATCCTGGC CAACATCCTG 
GAGGTGGAGC AGGCCAAGTG CTGGGGCTTC GACCAGTTCT TTGCGGAGAC CAGTGACATC 
CTGCAGCGAG TTGTCGTCCA TGTCTTCTCC CTGTCCCAGG CAGTCCTGCA CCACATCTAT 
ATCCATGCCC ACAACACGAT AGCCATTTTC CAGGAGGCCG TGCACAAGCA GACCAGTGTG 
GCCCCCCGAC ACCAGGAGTA CCTCTTTGAG GGTCACCTCT GTGTCCTCGA GCCCAGCGTC 
TCAGCACAGC ACATCGCCCA CACGACGGCA AGCAGCCCCC TGACCCTCTT CAGCACAGCC 
ATCCCTAAGG GGCTGGCCTT CAGGGACCCT GCTCTGGACG TCCCCAAGTT CGTCCCCAAA 
GTGGACCTGC AGGCGGATTA CAACACTGCC AAGGGCGTGT TGGGCGCCGG CTACCAGGCC 
CTGCGGCTGG CACGGGCCCT GCTGGATGGG CAGGAGCTAA TGTTTCGGGG GCTGCACTGG 
GTCATGGAGG TGCTCCAGGC CACATGCAGA CGGACTCTGG AAGTGGCAAG GACATCCCTC 
CTCTACCTCA GCAGCAGCCT GGGAACTGAG AGGTTCAGCA GCGTGGCTGG AACGCCTGAG 
ATCCAGGAAC TGAAGGCGGC TGCAGAACTG AGGTCCAGGC TGCGGACTCT AGCGGAGGTC 
CTCTCCAGAT GCTCCCAAAA TATCACGGAG ACCCAGGAGA GCCTGAGCAG CCTGAACCGG 
GAGCTGGTGA AGAGCCGGGA TCAGGTACAT GAGGACAGAA GCATCCAGCA GATTCAGTGC 
TGTTTGGACA AGATGAACTT CATCTACAAA CAGTTCAAGA AGTCTAGGAT GAGGCCAGGG 
CTTGGCTACA ACGAGGAGCA GATTCACAAG CTGGATAAGG TGAATTTCAG TCATTTAGCC 
AAAAGACTCC TGCAGGTGTT CCAGGAGGAG TGCGTGCAGA AGTATCAAGC GTCCTTAGTC 
ACACACGGCA AGAGGATGAG GGTGGTGCAC GAGACCAGGA ACCACCTGCG CCTGGTTGGC 
TGTTCTGTGG CTGCCTGTAA CACAGAAGCC CAGGGGGTCC AGGAGAGTCT CAGCAAGCTC 
CTGGAAGAGC TATCTCACCA GCTCCTTCAG GACCGAGCAA AGGGGGCTCA GGCCTCGCCG 
CCTCCCATAG CTCCTTACCC CAGCCCTACA CGAAAGGACC TGCTTCTCCA CATGCAAGAG 
CTCTGCGAGG GGATGAAGCT GCTGGCATCT GACCTCCTGG ACAACAACCG CATCATCGAA 
CGGCTAAATA GAGTCCCAGC ACCTCCTGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180250.1
CDS	715..2610
Translation	MEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Target ORF information:

RefSeq Version	NM_001193321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193321.1

ORF Insert Sequence:

ATGGAGTACT GCTCCAGTGG GAGCCTGCTG AGTGTGCTGG AGAGCCCTGA GAATGCCTTT 
GGGCTGCCTG AGGATGAGTT CCTGGTGGTG CTGCGCTGTG TGGTGGCCGG CATGAACCAC 
CTGCGGGAGA ACGGCATTGT GCATCGCGAC ATCAAGCCGG GGAACATCAT GCGCCTCGTA 
GGGGAGGAGG GGCAGAGCAT CTACAAGCTG ACAGACTTCG GCGCTGCCCG GGAGCTGGAT 
GATGATGAGA AGTTCGTCTC GGTCTATGGG ACTGAGGAGT ACCTGCATCC CGACATGTAT 
GAGCGGGCGG TGCTTCGAAA GCCCCAGCAA AAAGCGTTCG GGGTGACTGT GGATCTCTGG 
AGCATTGGAG TGACCTTGTA CCATGCAGCC ACTGGCAGCC TGCCCTTCAT CCCCTTTGGT 
GGGCCACGGC GGAACAAGGA GATCATGTAC CGGATCACCA CGGAGAAGCC GGCTGGGGCC 
ATTGCAGGTG CCCAGAGGCG GGAGAACGGG CCCCTGGAGT GGAGCTACAC CCTCCCCATC 
ACCTGCCAGC TGTCACTGGG GCTGCAGAGC CAGCTGGTGC CCATCCTGGC CAACATCCTG 
GAGGTGGAGC AGGCCAAGTG CTGGGGCTTC GACCAGTTCT TTGCGGAGAC CAGTGACATC 
CTGCAGCGAG TTGTCGTCCA TGTCTTCTCC CTGTCCCAGG CAGTCCTGCA CCACATCTAT 
ATCCATGCCC ACAACACGAT AGCCATTTTC CAGGAGGCCG TGCACAAGCA GACCAGTGTG 
GCCCCCCGAC ACCAGGAGTA CCTCTTTGAG GGTCACCTCT GTGTCCTCGA GCCCAGCGTC 
TCAGCACAGC ACATCGCCCA CACGACGGCA AGCAGCCCCC TGACCCTCTT CAGCACAGCC 
ATCCCTAAGG GGCTGGCCTT CAGGGACCCT GCTCTGGACG TCCCCAAGTT CGTCCCCAAA 
GTGGACCTGC AGGCGGATTA CAACACTGCC AAGGGCGTGT TGGGCGCCGG CTACCAGGCC 
CTGCGGCTGG CACGGGCCCT GCTGGATGGG CAGGAGCTAA TGTTTCGGGG GCTGCACTGG 
GTCATGGAGG TGCTCCAGGC CACATGCAGA CGGACTCTGG AAGTGGCAAG GACATCCCTC 
CTCTACCTCA GCAGCAGCCT GGGAACTGAG AGGTTCAGCA GCGTGGCTGG AACGCCTGAG 
ATCCAGGAAC TGAAGGCGGC TGCAGAACTG AGGTCCAGGC TGCGGACTCT AGCGGAGGTC 
CTCTCCAGAT GCTCCCAAAA TATCACGGAG ACCCAGGAGA GCCTGAGCAG CCTGAACCGG 
GAGCTGGTGA AGAGCCGGGA TCAGGTACAT GAGGACAGAA GCATCCAGCA GATTCAGTGC 
TGTTTGGACA AGATGAACTT CATCTACAAA CAGTTCAAGA AGTCTAGGAT GAGGCCAGGG 
CTTGGCTACA ACGAGGAGCA GATTCACAAG CTGGATAAGG TGAATTTCAG TCATTTAGCC 
AAAAGACTCC TGCAGGTGTT CCAGGAGGAG TGCGTGCAGA AGTATCAAGC GTCCTTAGTC 
ACACACGGCA AGAGGATGAG GGTGGTGCAC GAGACCAGGA ACCACCTGCG CCTGGTTGGC 
TGTTCTGTGG CTGCCTGTAA CACAGAAGCC CAGGGGGTCC AGGAGAGTCT CAGCAAGCTC 
CTGGAAGAGC TATCTCACCA GCTCCTTCAG GACCGAGCAA AGGGGGCTCA GGCCTCGCCG 
CCTCCCATAG CTCCTTACCC CAGCCCTACA CGAAAGGACC TGCTTCTCCA CATGCAAGAG 
CTCTGCGAGG GGATGAAGCT GCTGGCATCT GACCTCCTGG ACAACAACCG CATCATCGAA 
CGGCTAAATA GAGTCCCAGC ACCTCCTGAT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( NM_001193322.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180251.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26692
Clone ID Related Accession (Same CDS sequence)	NM_001193322.1 , NM_001193322.2
Accession Version	NM_001193322.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA999897.1, BC107591.1, DB152295.1, D63485.1 and CB243308.1. Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region, which shifts the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.197156.1, BC107591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCATGCAAG AGCTCTGCGA GGGGATGAAG CTGCTGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180251.1
CDS	579..2552
Translation	MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKHARALRGDEAAGI

Target ORF information:

RefSeq Version	NM_001193322.1
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193322.1

ORF Insert Sequence:

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCATGCAAG AGCTCTGCGA GGGGATGAAG CTGCTGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( NM_001193322.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001180251.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2, mRNA.

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CloneID	OHu26692
Clone ID Related Accession (Same CDS sequence)	NM_001193322.1 , NM_001193322.2
Accession Version	NM_001193322.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA999897.1, BC107591.1, DB152295.1, D63485.1 and CB243308.1. On May 31, 2019 this sequence version replaced NM_001193322.1. Transcript Variant: This variant (2) lacks an alternate segment in the 3' coding region, which shifts the reading frame, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus when it is compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.197156.1, BC107591.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCATGCAAG AGCTCTGCGA GGGGATGAAG CTGCTGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180251.1
CDS	346..2319
Translation	MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKHARALRGDEAAGI

Target ORF information:

RefSeq Version	NM_001193322.2
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193322.2

ORF Insert Sequence:

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCATGCAAG AGCTCTGCGA GGGGATGAAG CTGCTGGCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( XM_017002868.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858357.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant X2, mRNA.

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CloneID	OHu90822
Clone ID Related Accession (Same CDS sequence)	XM_017002868.1
Accession Version	XM_017002868.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCACC TGCGGGAGAA CGGCATTGTG CATCGCGACA TCAAGCCGGG GAACATCATG 
CGCCTCGTAG GGGAGGAGGG GCAGAGCATC TACAAGCTGA CAGACTTCGG CGCTGCCCGG 
GAGCTGGATG ATGATGAGAA GTTCGTCTCG GTCTATGGGA CTGAGGAGTA CCTGCATCCC 
GACATGTATG AGCGGGCGGT GCTTCGAAAG CCCCAGCAAA AAGCGTTCGG GGTGACTGTG 
GATCTCTGGA GCATTGGAGT GACCTTGTAC CATGCAGCCA CTGGCAGCCT GCCCTTCATC 
CCCTTTGGTG GGCCACGGCG GAACAAGGAG ATCATGTACC GGATCACCAC GGAGAAGCCG 
GCTGGGGCCA TTGCAGGTGC CCAGAGGCGG GAGAACGGGC CCCTGGAGTG GAGCTACACC 
CTCCCCATCA CCTGCCAGCT GTCACTGGGG CTGCAGAGCC AGCTGGTGCC CATCCTGGCC 
AACATCCTGG AGGTGGAGCA GGCCAAGTGC TGGGGCTTCG ACCAGTTCTT TGCGGAGACC 
AGTGACATCC TGCAGCGAGT TGTCGTCCAT GTCTTCTCCC TGTCCCAGGC AGTCCTGCAC 
CACATCTATA TCCATGCCCA CAACACGATA GCCATTTTCC AGGAGGCCGT GCACAAGCAG 
ACCAGTGTGG CCCCCCGACA CCAGGAGTAC CTCTTTGAGG GTCACCTCTG TGTCCTCGAG 
CCCAGCGTCT CAGCACAGCA CATCGCCCAC ACGACGGCAA GCAGCCCCCT GACCCTCTTC 
AGCACAGCCA TCCCTAAGGG GCTGGCCTTC AGGGACCCTG CTCTGGACGT CCCCAAGTTC 
GTCCCCAAAG TGGACCTGCA GGCGGATTAC AACACTGCCA AGGGCGTGTT GGGCGCCGGC 
TACCAGGCCC TGCGGCTGGC ACGGGCCCTG CTGGATGGGC AGGAGCTAAT GTTTCGGGGG 
CTGCACTGGG TCATGGAGGT GCTCCAGGCC ACATGCAGAC GGACTCTGGA AGTGGCAAGG 
ACATCCCTCC TCTACCTCAG CAGCAGCCTG GGAACTGAGA GGTTCAGCAG CGTGGCTGGA 
ACGCCTGAGA TCCAGGAACT GAAGGCGGCT GCAGAACTGA GGTCCAGGCT GCGGACTCTA 
GCGGAGGTCC TCTCCAGATG CTCCCAAAAT ATCACGGAGA CCCAGGAGAG CCTGAGCAGC 
CTGAACCGGG AGCTGGTGAA GAGCCGGGAT CAGGTACATG AGGACAGAAG CATCCAGCAG 
ATTCAGTGCT GTTTGGACAA GATGAACTTC ATCTACAAAC AGTTCAAGAA GTCTAGGATG 
AGGCCAGGGC TTGGCTACAA CGAGGAGCAG ATTCACAAGC TGGATAAGGT GAATTTCAGT 
CATTTAGCCA AAAGACTCCT GCAGGTGTTC CAGGAGGAGT GCGTGCAGAA GTATCAAGCG 
TCCTTAGTCA CACACGGCAA GAGGATGAGG GTGGTGCACG AGACCAGGAA CCACCTGCGC 
CTGGTTGGCT GTTCTGTGGC TGCCTGTAAC ACAGAAGCCC AGGGGGTCCA GGAGAGTCTC 
AGCAAGCTCC TGGAAGAGCT ATCTCACCAG CTCCTTCAGG ACCGAGCAAA GGGGGCTCAG 
GCCTCGCCGC CTCCCATAGC TCCTTACCCC AGCCCTACAC GAAAGGACCT GCTTCTCCAC 
ATGCAAGAGC TCTGCGAGGG GATGAAGCTG CTGGCATCTG ACCTCCTGGA CAACAACCGC 
ATCATCGAAC GGCTAAATAG AGTCCCAGCA CCTCCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858357.1
CDS	941..2725
Translation	MNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Target ORF information:

RefSeq Version	XM_017002868.1
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002868.1

ORF Insert Sequence:

ATGAACCACC TGCGGGAGAA CGGCATTGTG CATCGCGACA TCAAGCCGGG GAACATCATG 
CGCCTCGTAG GGGAGGAGGG GCAGAGCATC TACAAGCTGA CAGACTTCGG CGCTGCCCGG 
GAGCTGGATG ATGATGAGAA GTTCGTCTCG GTCTATGGGA CTGAGGAGTA CCTGCATCCC 
GACATGTATG AGCGGGCGGT GCTTCGAAAG CCCCAGCAAA AAGCGTTCGG GGTGACTGTG 
GATCTCTGGA GCATTGGAGT GACCTTGTAC CATGCAGCCA CTGGCAGCCT GCCCTTCATC 
CCCTTTGGTG GGCCACGGCG GAACAAGGAG ATCATGTACC GGATCACCAC GGAGAAGCCG 
GCTGGGGCCA TTGCAGGTGC CCAGAGGCGG GAGAACGGGC CCCTGGAGTG GAGCTACACC 
CTCCCCATCA CCTGCCAGCT GTCACTGGGG CTGCAGAGCC AGCTGGTGCC CATCCTGGCC 
AACATCCTGG AGGTGGAGCA GGCCAAGTGC TGGGGCTTCG ACCAGTTCTT TGCGGAGACC 
AGTGACATCC TGCAGCGAGT TGTCGTCCAT GTCTTCTCCC TGTCCCAGGC AGTCCTGCAC 
CACATCTATA TCCATGCCCA CAACACGATA GCCATTTTCC AGGAGGCCGT GCACAAGCAG 
ACCAGTGTGG CCCCCCGACA CCAGGAGTAC CTCTTTGAGG GTCACCTCTG TGTCCTCGAG 
CCCAGCGTCT CAGCACAGCA CATCGCCCAC ACGACGGCAA GCAGCCCCCT GACCCTCTTC 
AGCACAGCCA TCCCTAAGGG GCTGGCCTTC AGGGACCCTG CTCTGGACGT CCCCAAGTTC 
GTCCCCAAAG TGGACCTGCA GGCGGATTAC AACACTGCCA AGGGCGTGTT GGGCGCCGGC 
TACCAGGCCC TGCGGCTGGC ACGGGCCCTG CTGGATGGGC AGGAGCTAAT GTTTCGGGGG 
CTGCACTGGG TCATGGAGGT GCTCCAGGCC ACATGCAGAC GGACTCTGGA AGTGGCAAGG 
ACATCCCTCC TCTACCTCAG CAGCAGCCTG GGAACTGAGA GGTTCAGCAG CGTGGCTGGA 
ACGCCTGAGA TCCAGGAACT GAAGGCGGCT GCAGAACTGA GGTCCAGGCT GCGGACTCTA 
GCGGAGGTCC TCTCCAGATG CTCCCAAAAT ATCACGGAGA CCCAGGAGAG CCTGAGCAGC 
CTGAACCGGG AGCTGGTGAA GAGCCGGGAT CAGGTACATG AGGACAGAAG CATCCAGCAG 
ATTCAGTGCT GTTTGGACAA GATGAACTTC ATCTACAAAC AGTTCAAGAA GTCTAGGATG 
AGGCCAGGGC TTGGCTACAA CGAGGAGCAG ATTCACAAGC TGGATAAGGT GAATTTCAGT 
CATTTAGCCA AAAGACTCCT GCAGGTGTTC CAGGAGGAGT GCGTGCAGAA GTATCAAGCG 
TCCTTAGTCA CACACGGCAA GAGGATGAGG GTGGTGCACG AGACCAGGAA CCACCTGCGC 
CTGGTTGGCT GTTCTGTGGC TGCCTGTAAC ACAGAAGCCC AGGGGGTCCA GGAGAGTCTC 
AGCAAGCTCC TGGAAGAGCT ATCTCACCAG CTCCTTCAGG ACCGAGCAAA GGGGGCTCAG 
GCCTCGCCGC CTCCCATAGC TCCTTACCCC AGCCCTACAC GAAAGGACCT GCTTCTCCAC 
ATGCAAGAGC TCTGCGAGGG GATGAAGCTG CTGGCATCTG ACCTCCTGGA CAACAACCGC 
ATCATCGAAC GGCTAAATAG AGTCCCAGCA CCTCCTGATG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( NM_014002.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_054721.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.

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CloneID	OHu26712
Clone ID Related Accession (Same CDS sequence)	XM_005273356.2 , NM_014002.3 , NM_014002.4
Accession Version	NM_014002.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA999897.1, DA990116.1, DB152295.1, BC105923.1, D63485.1 and CB243308.1. On Jul 20, 2010 this sequence version replaced NM_014002.2. Transcript Variant: This variant (1) is the longest transcript and it encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D63485.1, SRR1660807.24458.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCTCCTGGA AGAGCTATCT CACCAGCTCC TTCAGGACCG AGCAAAGGGG 
GCTCAGGCCT CGCCGCCTCC CATAGCTCCT TACCCCAGCC CTACACGAAA GGACCTGCTT 
CTCCACATGC AAGAGCTCTG CGAGGGGATG AAGCTGCTGG CATCTGACCT CCTGGACAAC 
AACCGCATCA TCGAACGGCT AAATAGAGTC CCAGCACCTC CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054721.1
CDS	579..2729
Translation	MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Target ORF information:

RefSeq Version	NM_014002.3
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014002.3

ORF Insert Sequence:

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCTCCTGGA AGAGCTATCT CACCAGCTCC TTCAGGACCG AGCAAAGGGG 
GCTCAGGCCT CGCCGCCTCC CATAGCTCCT TACCCCAGCC CTACACGAAA GGACCTGCTT 
CTCCACATGC AAGAGCTCTG CGAGGGGATG AAGCTGCTGG CATCTGACCT CCTGGACAAC 
AACCGCATCA TCGAACGGCT AAATAGAGTC CCAGCACCTC CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( NM_014002.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_054721.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.

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CloneID	OHu26712
Clone ID Related Accession (Same CDS sequence)	XM_005273356.2 , NM_014002.3 , NM_014002.4
Accession Version	NM_014002.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-30
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA990116.1, DB152295.1, BC105923.1, D63485.1 and CB243308.1. On Nov 23, 2018 this sequence version replaced NM_014002.3. Transcript Variant: This variant (1) is the longest transcript and it encodes the longest protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D63485.1, BC105923.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000581977.7/ ENSP00000464030.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCTCCTGGA AGAGCTATCT CACCAGCTCC TTCAGGACCG AGCAAAGGGG 
GCTCAGGCCT CGCCGCCTCC CATAGCTCCT TACCCCAGCC CTACACGAAA GGACCTGCTT 
CTCCACATGC AAGAGCTCTG CGAGGGGATG AAGCTGCTGG CATCTGACCT CCTGGACAAC 
AACCGCATCA TCGAACGGCT AAATAGAGTC CCAGCACCTC CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_054721.1
CDS	346..2496
Translation	MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Target ORF information:

RefSeq Version	NM_014002.4
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014002.4

ORF Insert Sequence:

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCTCCTGGA AGAGCTATCT CACCAGCTCC TTCAGGACCG AGCAAAGGGG 
GCTCAGGCCT CGCCGCCTCC CATAGCTCCT TACCCCAGCC CTACACGAAA GGACCTGCTT 
CTCCACATGC AAGAGCTCTG CGAGGGGATG AAGCTGCTGG CATCTGACCT CCTGGACAAC 
AACCGCATCA TCGAACGGCT AAATAGAGTC CCAGCACCTC CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IKBKE ( XM_005273356.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005273413.1 Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant X1, mRNA.

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CloneID	OHu26712
Clone ID Related Accession (Same CDS sequence)	XM_005273356.2 , NM_014002.3 , NM_014002.4
Accession Version	XM_005273356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 3, 2014 this sequence version replaced XM_005273356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCTCCTGGA AGAGCTATCT CACCAGCTCC TTCAGGACCG AGCAAAGGGG 
GCTCAGGCCT CGCCGCCTCC CATAGCTCCT TACCCCAGCC CTACACGAAA GGACCTGCTT 
CTCCACATGC AAGAGCTCTG CGAGGGGATG AAGCTGCTGG CATCTGACCT CCTGGACAAC 
AACCGCATCA TCGAACGGCT AAATAGAGTC CCAGCACCTC CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005273413.1
CDS	575..2725
Translation	MQSTANYLWHTDDLLGQGATASVYKARNKKSGELVAVKVFNTTSYLRPREVQVREFEVLRKLNHQNIVKLFAVEETGGSRQKVLVMEYCSSGSLLSVLESPENAFGLPEDEFLVVLRCVVAGMNHLRENGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGTEEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFGGPRRNKEIMYRITTEKPAGAIAGAQRRENGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQAKCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAHNTIAIFQEAVHKQTSVAPRHQEYLFEGHLCVLEPSVSAQHIAHTTASSPLTLFSTAIPKGLAFRDPALDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDGQELMFRGLHWVMEVLQATCRRTLEVARTSLLYLSSSLGTERFSSVAGTPEIQELKAAAELRSRLRTLAEVLSRCSQNITETQESLSSLNRELVKSRDQVHEDRSIQQIQCCLDKMNFIYKQFKKSRMRPGLGYNEEQIHKLDKVNFSHLAKRLLQVFQEECVQKYQASLVTHGKRMRVVHETRNHLRLVGCSVAACNTEAQGVQESLSKLLEELSHQLLQDRAKGAQASPPPIAPYPSPTRKDLLLHMQELCEGMKLLASDLLDNNRIIERLNRVPAPPDV

Target ORF information:

RefSeq Version	XM_005273356.2
Organism	Homo sapiens(human)
Definition	Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005273356.2

ORF Insert Sequence:

ATGCAGAGCA CAGCCAATTA CCTGTGGCAC ACAGATGACC TGCTGGGGCA GGGGGCCACT 
GCCAGTGTGT ACAAGGCCCG CAACAAGAAA TCCGGAGAGC TGGTTGCTGT GAAGGTCTTC 
AACACTACCA GCTACCTGCG GCCCCGCGAG GTGCAGGTGA GGGAGTTTGA GGTCCTGCGG 
AAGCTGAACC ACCAGAACAT TGTCAAGCTC TTTGCGGTGG AGGAGACGGG CGGAAGCCGG 
CAGAAGGTAC TGGTGATGGA GTACTGCTCC AGTGGGAGCC TGCTGAGTGT GCTGGAGAGC 
CCTGAGAATG CCTTTGGGCT GCCTGAGGAT GAGTTCCTGG TGGTGCTGCG CTGTGTGGTG 
GCCGGCATGA ACCACCTGCG GGAGAACGGC ATTGTGCATC GCGACATCAA GCCGGGGAAC 
ATCATGCGCC TCGTAGGGGA GGAGGGGCAG AGCATCTACA AGCTGACAGA CTTCGGCGCT 
GCCCGGGAGC TGGATGATGA TGAGAAGTTC GTCTCGGTCT ATGGGACTGA GGAGTACCTG 
CATCCCGACA TGTATGAGCG GGCGGTGCTT CGAAAGCCCC AGCAAAAAGC GTTCGGGGTG 
ACTGTGGATC TCTGGAGCAT TGGAGTGACC TTGTACCATG CAGCCACTGG CAGCCTGCCC 
TTCATCCCCT TTGGTGGGCC ACGGCGGAAC AAGGAGATCA TGTACCGGAT CACCACGGAG 
AAGCCGGCTG GGGCCATTGC AGGTGCCCAG AGGCGGGAGA ACGGGCCCCT GGAGTGGAGC 
TACACCCTCC CCATCACCTG CCAGCTGTCA CTGGGGCTGC AGAGCCAGCT GGTGCCCATC 
CTGGCCAACA TCCTGGAGGT GGAGCAGGCC AAGTGCTGGG GCTTCGACCA GTTCTTTGCG 
GAGACCAGTG ACATCCTGCA GCGAGTTGTC GTCCATGTCT TCTCCCTGTC CCAGGCAGTC 
CTGCACCACA TCTATATCCA TGCCCACAAC ACGATAGCCA TTTTCCAGGA GGCCGTGCAC 
AAGCAGACCA GTGTGGCCCC CCGACACCAG GAGTACCTCT TTGAGGGTCA CCTCTGTGTC 
CTCGAGCCCA GCGTCTCAGC ACAGCACATC GCCCACACGA CGGCAAGCAG CCCCCTGACC 
CTCTTCAGCA CAGCCATCCC TAAGGGGCTG GCCTTCAGGG ACCCTGCTCT GGACGTCCCC 
AAGTTCGTCC CCAAAGTGGA CCTGCAGGCG GATTACAACA CTGCCAAGGG CGTGTTGGGC 
GCCGGCTACC AGGCCCTGCG GCTGGCACGG GCCCTGCTGG ATGGGCAGGA GCTAATGTTT 
CGGGGGCTGC ACTGGGTCAT GGAGGTGCTC CAGGCCACAT GCAGACGGAC TCTGGAAGTG 
GCAAGGACAT CCCTCCTCTA CCTCAGCAGC AGCCTGGGAA CTGAGAGGTT CAGCAGCGTG 
GCTGGAACGC CTGAGATCCA GGAACTGAAG GCGGCTGCAG AACTGAGGTC CAGGCTGCGG 
ACTCTAGCGG AGGTCCTCTC CAGATGCTCC CAAAATATCA CGGAGACCCA GGAGAGCCTG 
AGCAGCCTGA ACCGGGAGCT GGTGAAGAGC CGGGATCAGG TACATGAGGA CAGAAGCATC 
CAGCAGATTC AGTGCTGTTT GGACAAGATG AACTTCATCT ACAAACAGTT CAAGAAGTCT 
AGGATGAGGC CAGGGCTTGG CTACAACGAG GAGCAGATTC ACAAGCTGGA TAAGGTGAAT 
TTCAGTCATT TAGCCAAAAG ACTCCTGCAG GTGTTCCAGG AGGAGTGCGT GCAGAAGTAT 
CAAGCGTCCT TAGTCACACA CGGCAAGAGG ATGAGGGTGG TGCACGAGAC CAGGAACCAC 
CTGCGCCTGG TTGGCTGTTC TGTGGCTGCC TGTAACACAG AAGCCCAGGG GGTCCAGGAG 
AGTCTCAGCA AGCTCCTGGA AGAGCTATCT CACCAGCTCC TTCAGGACCG AGCAAAGGGG 
GCTCAGGCCT CGCCGCCTCC CATAGCTCCT TACCCCAGCC CTACACGAAA GGACCTGCTT 
CTCCACATGC AAGAGCTCTG CGAGGGGATG AAGCTGCTGG CATCTGACCT CCTGGACAAC 
AACCGCATCA TCGAACGGCT AAATAGAGTC CCAGCACCTC CTGATGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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IKBKE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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