PDIA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDIA4
Entrez Gene ID	9601
Full Name	protein disulfide isomerase family A member 4
Synonyms	ERP70,ERP72,ERp-72
General protein information	Preferred Names protein disulfide isomerase family A member 4 Names protein disulfide-isomerase A4 ER protein 70 ER protein 72 endoplasmic reticulum resident protein 70 endoplasmic reticulum resident protein 72 protein disulfide isomerase related protein (calcium-binding protein, intestinal-related) protein disulfide isomerase-associated 4 testicular tissue protein Li 137
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q36.1
Summary	This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, two TRX-like domains and a C-terminal ER-retention sequence. This protein, when bound to cyclophilin B, enhances the rate of immunoglobulin G intermolecular disulfide bonding and antibody assembly. [provided by RefSeq, Dec 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001371244.1	NP_001358173.1	protein disulfide-isomerase A4 isoform 1 precursor
NM_004911.5	NP_004902.1	protein disulfide-isomerase A4 isofrom 2 precursor
XM_006716185.2	XP_006716248.1	protein disulfide-isomerase A4 isoform X1
NM_004911.4	NP_004902.1	protein disulfide-isomerase A4 precursor
NM_001371245.1	NP_001358174.1	protein disulfide-isomerase A4 isoform 3 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDIA4	XP_848238.3
Bos taurus (cattle)	PDIA4	NP_001039344.1
Danio rerio (zebrafish)	pdia4	NP_956073.1
Arabidopsis thaliana (thale cress)	PDIL1-2	NP_177875.1
Caenorhabditis elegans (roundworm)	C14B9.2	NP_498775.2
Xenopus tropicalis (tropical clawed frog)	pdia4	XP_002941424.1
Pan troglodytes (chimpanzee)	PDIA4	XP_003318916.1
Mus musculus (house mouse)	Pdia4	NP_033917.2
Rattus norvegicus (Norway rat)	Pdia4	NP_446301.1
Arabidopsis thaliana (thale cress)	PDIL1-1	NP_173594.1
Gallus gallus (chicken)	PDIA4	NP_001006370.1
Homo sapiens (human)	PDIA4	NP_004902.1
Oryza sativa (rice)	Os11g0199200	NP_001067436.1

Gene Ontology
Bibliography

Related articles in PubMed

Protein disulfide isomerase a4 acts as a novel regulator of cancer growth through the procaspase pathway.
Kuo TF, Chen TY, Jiang ST, Chen KW, Chiang YM, Hsu YJ, Liu YJ, Chen HM, Yokoyama KK, Tsai KC, Yeh HH, Chen YR, Yang MT, Yang CY, Yang WC
Oncogene36(39)5484-5496(2017 Sep)

Interleukin-11 up-regulates endoplasmic reticulum stress induced target, PDIA4 in human first trimester placenta and in?vivo in mice.
Winship AL, Sorby K, Correia J, Rainczuk A, Yap J, Dimitriadis E
Placenta5392-100(2017 May)

A novel potential biomarker for metabolic syndrome in Chinese adults: Circulating protein disulfide isomerase family A, member 4.
Chien CY, Hung YJ, Shieh YS, Hsieh CH, Lu CH, Lin FH, Su SC, Lee CH
PloS one12(6)e0179963(2017)

Transient covalent interactions of newly synthesized thyroglobulin with oxidoreductases of the endoplasmic reticulum.
Di Jeso B, Morishita Y, Treglia AS, Lofrumento DD, Nicolardi G, Beguinot F, Kellogg AP, Arvan P
The Journal of biological chemistry289(16)11488-96(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PDIA4 as a negative regulator of cancer cell apoptosis.
Title: Protein disulfide isomerase a4 acts as a novel regulator of cancer growth through the procaspase pathway.

Participants with metabolic syndrome (MetS) had significantly higher serum PDIA4 levels than those without MetS (P<0.001). After adjustments, the individuals with the highest PDIA4 tertile were associated with a higher risk of MetS than those with the lowest tertile (OR = 4.83, 95% CI: 2.71-8.60).
Title: A novel potential biomarker for metabolic syndrome in Chinese adults: Circulating protein disulfide isomerase family A, member 4.

CDDP-resistant non-small lung cancer cells undergo profound remodeling of their endoplasmic reticulum (ER) proteome (>80 proteins identified by proteomics) and exhibit a dramatic overexpression of two protein disulfide isomerases, PDIA4 and PDIA6.
Title: The protein disulfide isomerases PDIA4 and PDIA6 mediate resistance to cisplatin-induced cell death in lung adenocarcinoma.

the faster migrating Tg adduct C primarily engages the CaBP1/P5 oxidoreductase, whereas the slower migrating Tg adduct A primarily engages ERp72.
Title: Transient covalent interactions of newly synthesized thyroglobulin with oxidoreductases of the endoplasmic reticulum.

novel esophageal squamous cell carcinoma marker PDIA4 was identified by mass spectrometry and immunohistochemical analysis
Title: Quantitative tissue proteomics of esophageal squamous cell carcinoma for novel biomarker discovery.

The following PDIA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDIA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04678	NM_004911.5 Latest version!	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu41878	NM_001371244.1 Latest version!	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu41878	XM_006716185.2 Latest version!	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu108641	NM_001371245.1 Latest version!	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu04678	NM_004911.4	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDIA4 ( NM_004911.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004902.1 Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04678
Clone ID Related Accession (Same CDS sequence)	NM_004911.5 , NM_004911.4
Accession Version	NM_004911.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	protein disulfide-isomerase A4 isofrom 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU139362.1, BC000425.2, CX871856.1 and BC001928.1. On Nov 22, 2018 this sequence version replaced NM_004911.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011754.2, SRR1803617.95934.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000652332.1/ ENSP00000499129.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GGGCTCCACC 
CAGGACTCGG CCATCAAGGA CTTCGTGCTG AAGTACGCCC TGCCCCTGGT TGGCCACCGC 
AAGGTGTCAA ACGATGCTAA GCGCTACACC AGGCGCCCCC TGGTGGTCGT CTACTACAGT 
GTGGACTTCA GCTTTGATTA CAGAGCTGCA ACTCAGTTTT GGCGGAGCAA AGTCCTAGAG 
GTGGCCAAGG ACTTCCCTGA GTACACCTTT GCCATTGCGG ACGAAGAGGA CTATGCTGGG 
GAGGTGAAGG ACCTGGGGCT CAGCGAGAGT GGGGAGGATG TCAATGCCGC CATCCTGGAC 
GAGAGTGGGA AGAAGTTCGC CATGGAGCCA GAGGAGTTTG ACTCTGACAC CCTCCGCGAG 
TTTGTCACTG CTTTCAAAAA AGGAAAACTG AAGCCAGTCA TCAAATCCCA GCCAGTGCCC 
AAGAACAACA AGGGACCCGT CAAGGTCGTG GTGGGAAAGA CCTTTGACTC CATTGTGATG 
GACCCCAAGA AGGACGTCCT CATCGAGTTC TACGCGCCAT GGTGCGGGCA CTGCAAGCAG 
CTAGAGCCCG TGTACAACAG CCTGGCCAAG AAGTACAAGG GCCAAAAGGG CCTGGTCATC 
GCCAAGATGG ACGCCACTGC CAACGACGTC CCCAGCGACC GCTATAAGGT GGAGGGCTTC 
CCCACCATCT ACTTCGCCCC CAGTGGGGAC AAAAAGAACC CAGTTAAATT TGAGGGTGGA 
GACAGAGATC TGGAGCATTT GAGCAAGTTT ATAGAAGAAC ATGCCACAAA ACTGAGCAGG 
ACCAAGGAAG AGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004902.1
CDS	98..2035
Translation	MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

Target ORF information:

RefSeq Version	NM_004911.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004911.5

ORF Insert Sequence:

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GGGCTCCACC 
CAGGACTCGG CCATCAAGGA CTTCGTGCTG AAGTACGCCC TGCCCCTGGT TGGCCACCGC 
AAGGTGTCAA ACGATGCTAA GCGCTACACC AGGCGCCCCC TGGTGGTCGT CTACTACAGT 
GTGGACTTCA GCTTTGATTA CAGAGCTGCA ACTCAGTTTT GGCGGAGCAA AGTCCTAGAG 
GTGGCCAAGG ACTTCCCTGA GTACACCTTT GCCATTGCGG ACGAAGAGGA CTATGCTGGG 
GAGGTGAAGG ACCTGGGGCT CAGCGAGAGT GGGGAGGATG TCAATGCCGC CATCCTGGAC 
GAGAGTGGGA AGAAGTTCGC CATGGAGCCA GAGGAGTTTG ACTCTGACAC CCTCCGCGAG 
TTTGTCACTG CTTTCAAAAA AGGAAAACTG AAGCCAGTCA TCAAATCCCA GCCAGTGCCC 
AAGAACAACA AGGGACCCGT CAAGGTCGTG GTGGGAAAGA CCTTTGACTC CATTGTGATG 
GACCCCAAGA AGGACGTCCT CATCGAGTTC TACGCGCCAT GGTGCGGGCA CTGCAAGCAG 
CTAGAGCCCG TGTACAACAG CCTGGCCAAG AAGTACAAGG GCCAAAAGGG CCTGGTCATC 
GCCAAGATGG ACGCCACTGC CAACGACGTC CCCAGCGACC GCTATAAGGT GGAGGGCTTC 
CCCACCATCT ACTTCGCCCC CAGTGGGGAC AAAAAGAACC CAGTTAAATT TGAGGGTGGA 
GACAGAGATC TGGAGCATTT GAGCAAGTTT ATAGAAGAAC ATGCCACAAA ACTGAGCAGG 
ACCAAGGAAG AGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDIA4 ( NM_004911.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004902.1 Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), mRNA.

Price & Availability↑

CloneID	OHu04678
Clone ID Related Accession (Same CDS sequence)	NM_004911.5 , NM_004911.4
Accession Version	NM_004911.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	protein disulfide-isomerase A4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU139362.1, BC000425.2, CX871856.1 and BC001928.1. On Sep 24, 2007 this sequence version replaced NM_004911.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011754.2, SRR1660807.178910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GGGCTCCACC 
CAGGACTCGG CCATCAAGGA CTTCGTGCTG AAGTACGCCC TGCCCCTGGT TGGCCACCGC 
AAGGTGTCAA ACGATGCTAA GCGCTACACC AGGCGCCCCC TGGTGGTCGT CTACTACAGT 
GTGGACTTCA GCTTTGATTA CAGAGCTGCA ACTCAGTTTT GGCGGAGCAA AGTCCTAGAG 
GTGGCCAAGG ACTTCCCTGA GTACACCTTT GCCATTGCGG ACGAAGAGGA CTATGCTGGG 
GAGGTGAAGG ACCTGGGGCT CAGCGAGAGT GGGGAGGATG TCAATGCCGC CATCCTGGAC 
GAGAGTGGGA AGAAGTTCGC CATGGAGCCA GAGGAGTTTG ACTCTGACAC CCTCCGCGAG 
TTTGTCACTG CTTTCAAAAA AGGAAAACTG AAGCCAGTCA TCAAATCCCA GCCAGTGCCC 
AAGAACAACA AGGGACCCGT CAAGGTCGTG GTGGGAAAGA CCTTTGACTC CATTGTGATG 
GACCCCAAGA AGGACGTCCT CATCGAGTTC TACGCGCCAT GGTGCGGGCA CTGCAAGCAG 
CTAGAGCCCG TGTACAACAG CCTGGCCAAG AAGTACAAGG GCCAAAAGGG CCTGGTCATC 
GCCAAGATGG ACGCCACTGC CAACGACGTC CCCAGCGACC GCTATAAGGT GGAGGGCTTC 
CCCACCATCT ACTTCGCCCC CAGTGGGGAC AAAAAGAACC CAGTTAAATT TGAGGGTGGA 
GACAGAGATC TGGAGCATTT GAGCAAGTTT ATAGAAGAAC ATGCCACAAA ACTGAGCAGG 
ACCAAGGAAG AGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004902.1
CDS	283..2220
Translation	MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

Target ORF information:

RefSeq Version	NM_004911.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004911.4

ORF Insert Sequence:

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GGGCTCCACC 
CAGGACTCGG CCATCAAGGA CTTCGTGCTG AAGTACGCCC TGCCCCTGGT TGGCCACCGC 
AAGGTGTCAA ACGATGCTAA GCGCTACACC AGGCGCCCCC TGGTGGTCGT CTACTACAGT 
GTGGACTTCA GCTTTGATTA CAGAGCTGCA ACTCAGTTTT GGCGGAGCAA AGTCCTAGAG 
GTGGCCAAGG ACTTCCCTGA GTACACCTTT GCCATTGCGG ACGAAGAGGA CTATGCTGGG 
GAGGTGAAGG ACCTGGGGCT CAGCGAGAGT GGGGAGGATG TCAATGCCGC CATCCTGGAC 
GAGAGTGGGA AGAAGTTCGC CATGGAGCCA GAGGAGTTTG ACTCTGACAC CCTCCGCGAG 
TTTGTCACTG CTTTCAAAAA AGGAAAACTG AAGCCAGTCA TCAAATCCCA GCCAGTGCCC 
AAGAACAACA AGGGACCCGT CAAGGTCGTG GTGGGAAAGA CCTTTGACTC CATTGTGATG 
GACCCCAAGA AGGACGTCCT CATCGAGTTC TACGCGCCAT GGTGCGGGCA CTGCAAGCAG 
CTAGAGCCCG TGTACAACAG CCTGGCCAAG AAGTACAAGG GCCAAAAGGG CCTGGTCATC 
GCCAAGATGG ACGCCACTGC CAACGACGTC CCCAGCGACC GCTATAAGGT GGAGGGCTTC 
CCCACCATCT ACTTCGCCCC CAGTGGGGAC AAAAAGAACC CAGTTAAATT TGAGGGTGGA 
GACAGAGATC TGGAGCATTT GAGCAAGTTT ATAGAAGAAC ATGCCACAAA ACTGAGCAGG 
ACCAAGGAAG AGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDIA4 ( NM_001371244.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358173.1 Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 1, mRNA.

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CloneID	OHu41878
Clone ID Related Accession (Same CDS sequence)	NM_001371244.1 , XM_006716185.2
Accession Version	NM_001371244.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	protein disulfide-isomerase A4 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093743.2. On Jul 2, 2019 this sequence version replaced XM_006716185.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.40318.1, SRR1803617.47039.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GCAGGGCTCC 
ACCCAGGACT CGGCCATCAA GGACTTCGTG CTGAAGTACG CCCTGCCCCT GGTTGGCCAC 
CGCAAGGTGT CAAACGATGC TAAGCGCTAC ACCAGGCGCC CCCTGGTGGT CGTCTACTAC 
AGTGTGGACT TCAGCTTTGA TTACAGAGCT GCAACTCAGT TTTGGCGGAG CAAAGTCCTA 
GAGGTGGCCA AGGACTTCCC TGAGTACACC TTTGCCATTG CGGACGAAGA GGACTATGCT 
GGGGAGGTGA AGGACCTGGG GCTCAGCGAG AGTGGGGAGG ATGTCAATGC CGCCATCCTG 
GACGAGAGTG GGAAGAAGTT CGCCATGGAG CCAGAGGAGT TTGACTCTGA CACCCTCCGC 
GAGTTTGTCA CTGCTTTCAA AAAAGGAAAA CTGAAGCCAG TCATCAAATC CCAGCCAGTG 
CCCAAGAACA ACAAGGGACC CGTCAAGGTC GTGGTGGGAA AGACCTTTGA CTCCATTGTG 
ATGGACCCCA AGAAGGACGT CCTCATCGAG TTCTACGCGC CATGGTGCGG GCACTGCAAG 
CAGCTAGAGC CCGTGTACAA CAGCCTGGCC AAGAAGTACA AGGGCCAAAA GGGCCTGGTC 
ATCGCCAAGA TGGACGCCAC TGCCAACGAC GTCCCCAGCG ACCGCTATAA GGTGGAGGGC 
TTCCCCACCA TCTACTTCGC CCCCAGTGGG GACAAAAAGA ACCCAGTTAA ATTTGAGGGT 
GGAGACAGAG ATCTGGAGCA TTTGAGCAAG TTTATAGAAG AACATGCCAC AAAACTGAGC 
AGGACCAAGG AAGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358173.1
CDS	98..2038
Translation	MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

Target ORF information:

RefSeq Version	NM_001371244.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371244.1

ORF Insert Sequence:

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GCAGGGCTCC 
ACCCAGGACT CGGCCATCAA GGACTTCGTG CTGAAGTACG CCCTGCCCCT GGTTGGCCAC 
CGCAAGGTGT CAAACGATGC TAAGCGCTAC ACCAGGCGCC CCCTGGTGGT CGTCTACTAC 
AGTGTGGACT TCAGCTTTGA TTACAGAGCT GCAACTCAGT TTTGGCGGAG CAAAGTCCTA 
GAGGTGGCCA AGGACTTCCC TGAGTACACC TTTGCCATTG CGGACGAAGA GGACTATGCT 
GGGGAGGTGA AGGACCTGGG GCTCAGCGAG AGTGGGGAGG ATGTCAATGC CGCCATCCTG 
GACGAGAGTG GGAAGAAGTT CGCCATGGAG CCAGAGGAGT TTGACTCTGA CACCCTCCGC 
GAGTTTGTCA CTGCTTTCAA AAAAGGAAAA CTGAAGCCAG TCATCAAATC CCAGCCAGTG 
CCCAAGAACA ACAAGGGACC CGTCAAGGTC GTGGTGGGAA AGACCTTTGA CTCCATTGTG 
ATGGACCCCA AGAAGGACGT CCTCATCGAG TTCTACGCGC CATGGTGCGG GCACTGCAAG 
CAGCTAGAGC CCGTGTACAA CAGCCTGGCC AAGAAGTACA AGGGCCAAAA GGGCCTGGTC 
ATCGCCAAGA TGGACGCCAC TGCCAACGAC GTCCCCAGCG ACCGCTATAA GGTGGAGGGC 
TTCCCCACCA TCTACTTCGC CCCCAGTGGG GACAAAAAGA ACCCAGTTAA ATTTGAGGGT 
GGAGACAGAG ATCTGGAGCA TTTGAGCAAG TTTATAGAAG AACATGCCAC AAAACTGAGC 
AGGACCAAGG AAGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDIA4 ( XM_006716185.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006716248.1 Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant X1, mRNA.

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CloneID	OHu41878
Clone ID Related Accession (Same CDS sequence)	NM_001371244.1 , XM_006716185.2
Accession Version	XM_006716185.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1941bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	protein disulfide-isomerase A4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006716185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GCAGGGCTCC 
ACCCAGGACT CGGCCATCAA GGACTTCGTG CTGAAGTACG CCCTGCCCCT GGTTGGCCAC 
CGCAAGGTGT CAAACGATGC TAAGCGCTAC ACCAGGCGCC CCCTGGTGGT CGTCTACTAC 
AGTGTGGACT TCAGCTTTGA TTACAGAGCT GCAACTCAGT TTTGGCGGAG CAAAGTCCTA 
GAGGTGGCCA AGGACTTCCC TGAGTACACC TTTGCCATTG CGGACGAAGA GGACTATGCT 
GGGGAGGTGA AGGACCTGGG GCTCAGCGAG AGTGGGGAGG ATGTCAATGC CGCCATCCTG 
GACGAGAGTG GGAAGAAGTT CGCCATGGAG CCAGAGGAGT TTGACTCTGA CACCCTCCGC 
GAGTTTGTCA CTGCTTTCAA AAAAGGAAAA CTGAAGCCAG TCATCAAATC CCAGCCAGTG 
CCCAAGAACA ACAAGGGACC CGTCAAGGTC GTGGTGGGAA AGACCTTTGA CTCCATTGTG 
ATGGACCCCA AGAAGGACGT CCTCATCGAG TTCTACGCGC CATGGTGCGG GCACTGCAAG 
CAGCTAGAGC CCGTGTACAA CAGCCTGGCC AAGAAGTACA AGGGCCAAAA GGGCCTGGTC 
ATCGCCAAGA TGGACGCCAC TGCCAACGAC GTCCCCAGCG ACCGCTATAA GGTGGAGGGC 
TTCCCCACCA TCTACTTCGC CCCCAGTGGG GACAAAAAGA ACCCAGTTAA ATTTGAGGGT 
GGAGACAGAG ATCTGGAGCA TTTGAGCAAG TTTATAGAAG AACATGCCAC AAAACTGAGC 
AGGACCAAGG AAGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006716248.1
CDS	283..2223
Translation	MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPWCGHCKKLAPEYEKAAKELSKRSPPIPLAKVDATAETDLAKRFDVSGYPTLKIFRKGRPYDYNGPREKYGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

Target ORF information:

RefSeq Version	XM_006716185.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006716185.2

ORF Insert Sequence:

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGGTGTGG ACACTGCAAG AAACTTGCCC CCGAGTATGA GAAGGCCGCC 
AAGGAGCTCA GCAAGCGTTC TCCTCCAATT CCCCTGGCAA AGGTCGACGC CACCGCAGAA 
ACAGACCTGG CCAAGAGGTT TGATGTCTCT GGCTATCCCA CCCTGAAAAT TTTCCGCAAA 
GGAAGGCCTT ATGACTACAA CGGCCCACGA GAAAAATATG GAATCGTTGA TTACATGATC 
GAGCAGTCCG GGCCTCCCTC CAAGGAGATT CTGACCCTGA AGCAGGTCCA GGAGTTCCTG 
AAGGATGGAG ACGATGTCAT CATCATCGGG GTCTTTAAGG GGGAGAGTGA CCCAGCCTAC 
CAGCAATACC AGGATGCCGC TAACAACCTG AGAGAAGATT ACAAATTTCA CCACACTTTC 
AGCACAGAAA TAGCAAAGTT CTTGAAAGTC TCCCAGGGGC AGTTGGTTGT AATGCAGCCT 
GAGAAATTCC AGTCCAAGTA TGAGCCCCGG AGCCACATGA TGGACGTCCA GCAGGGCTCC 
ACCCAGGACT CGGCCATCAA GGACTTCGTG CTGAAGTACG CCCTGCCCCT GGTTGGCCAC 
CGCAAGGTGT CAAACGATGC TAAGCGCTAC ACCAGGCGCC CCCTGGTGGT CGTCTACTAC 
AGTGTGGACT TCAGCTTTGA TTACAGAGCT GCAACTCAGT TTTGGCGGAG CAAAGTCCTA 
GAGGTGGCCA AGGACTTCCC TGAGTACACC TTTGCCATTG CGGACGAAGA GGACTATGCT 
GGGGAGGTGA AGGACCTGGG GCTCAGCGAG AGTGGGGAGG ATGTCAATGC CGCCATCCTG 
GACGAGAGTG GGAAGAAGTT CGCCATGGAG CCAGAGGAGT TTGACTCTGA CACCCTCCGC 
GAGTTTGTCA CTGCTTTCAA AAAAGGAAAA CTGAAGCCAG TCATCAAATC CCAGCCAGTG 
CCCAAGAACA ACAAGGGACC CGTCAAGGTC GTGGTGGGAA AGACCTTTGA CTCCATTGTG 
ATGGACCCCA AGAAGGACGT CCTCATCGAG TTCTACGCGC CATGGTGCGG GCACTGCAAG 
CAGCTAGAGC CCGTGTACAA CAGCCTGGCC AAGAAGTACA AGGGCCAAAA GGGCCTGGTC 
ATCGCCAAGA TGGACGCCAC TGCCAACGAC GTCCCCAGCG ACCGCTATAA GGTGGAGGGC 
TTCCCCACCA TCTACTTCGC CCCCAGTGGG GACAAAAAGA ACCCAGTTAA ATTTGAGGGT 
GGAGACAGAG ATCTGGAGCA TTTGAGCAAG TTTATAGAAG AACATGCCAC AAAACTGAGC 
AGGACCAAGG AAGAGCTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDIA4 ( NM_001371245.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358174.1 Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108641
Clone ID Related Accession (Same CDS sequence)	NM_001371245.1
Accession Version	NM_001371245.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	protein disulfide-isomerase A4 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC093743.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.34508.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGTTTCCT CTTGGCAGGT GTGGACACTG CAAGAAACTT GCCCCCGAGT 
ATGAGAAGGC CGCCAAGGAG CTCAGCAAGC GTTCTCCTCC AATTCCCCTG GCAAAGGTCG 
ACGCCACCGC AGAAACAGAC CTGGCCAAGA GGAATCGTTG ATTACATGAT CGAGCAGTCC 
GGGCCTCCCT CCAAGGAGAT TCTGACCCTG AAGCAGGTCC AGGAGTTCCT GAAGGATGGA 
GACGATGTCA TCATCATCGG GGTCTTTAAG GGGGAGAGTG ACCCAGCCTA CCAGCAATAC 
CAGGATGCCG CTAACAACCT GAGAGAAGAT TACAAATTTC ACCACACTTT CAGCACAGAA 
ATAGCAAAGT TCTTGAAAGT CTCCCAGGGG CAGTTGGTTG TAATGCAGCC TGAGAAATTC 
CAGTCCAAGT ATGAGCCCCG GAGCCACATG ATGGACGTCC AGGGCTCCAC CCAGGACTCG 
GCCATCAAGG ACTTCGTGCT GAAGTACGCC CTGCCCCTGG TTGGCCACCG CAAGGTGTCA 
AACGATGCTA AGCGCTACAC CAGGCGCCCC CTGGTGGTCG TCTACTACAG TGTGGACTTC 
AGCTTTGATT ACAGAGCTGC AACTCAGTTT TGGCGGAGCA AAGTCCTAGA GGTGGCCAAG 
GACTTCCCTG AGTACACCTT TGCCATTGCG GACGAAGAGG ACTATGCTGG GGAGGTGAAG 
GACCTGGGGC TCAGCGAGAG TGGGGAGGAT GTCAATGCCG CCATCCTGGA CGAGAGTGGG 
AAGAAGTTCG CCATGGAGCC AGAGGAGTTT GACTCTGACA CCCTCCGCGA GTTTGTCACT 
GCTTTCAAAA AAGGAAAACT GAAGCCAGTC ATCAAATCCC AGCCAGTGCC CAAGAACAAC 
AAGGGACCCG TCAAGGTCGT GGTGGGAAAG ACCTTTGACT CCATTGTGAT GGACCCCAAG 
AAGGACGTCC TCATCGAGTT CTACGCGCCA TGGTGCGGGC ACTGCAAGCA GCTAGAGCCC 
GTGTACAACA GCCTGGCCAA GAAGTACAAG GGCCAAAAGG GCCTGGTCAT CGCCAAGATG 
GACGCCACTG CCAACGACGT CCCCAGCGAC CGCTATAAGG TGGAGGGCTT CCCCACCATC 
TACTTCGCCC CCAGTGGGGA CAAAAAGAAC CCAGTTAAAT TTGAGGGTGG AGACAGAGAT 
CTGGAGCATT TGAGCAAGTT TATAGAAGAA CATGCCACAA AACTGAGCAG GACCAAGGAA 
GAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358174.1
CDS	98..1966
Translation	MRPRKAFLLLLLLGLVQLLAVAGAEGPDEDSSNRENAIEDEEEEEEEDDDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIANILKDKDPPIPVAKIDATSASVLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLVLTKENFDEVVNDADIILVEFYAPCFLLAGVDTARNLPPSMRRPPRSSASVLLQFPWQRSTPPQKQTWPRGIVDYMIEQSGPPSKEILTLKQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL

Target ORF information:

RefSeq Version	NM_001371245.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein disulfide isomerase family A member 4 (PDIA4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371245.1

ORF Insert Sequence:

ATGAGGCCCC GGAAAGCCTT CCTGCTCCTG CTGCTCTTGG GGCTGGTGCA GCTGCTGGCC 
GTGGCGGGTG CCGAGGGCCC GGACGAGGAT TCTTCTAACA GAGAAAATGC CATTGAGGAT 
GAAGAGGAGG AGGAGGAGGA AGATGATGAT GAGGAAGAAG ACGACTTGGA AGTTAAGGAA 
GAAAATGGAG TCTTGGTCCT AAATGATGCA AACTTTGATA ATTTTGTGGC TGACAAAGAC 
ACAGTGCTGC TGGAGTTTTA TGCTCCATGG TGTGGACATT GCAAGCAGTT TGCTCCGGAA 
TATGAAAAAA TTGCCAACAT ATTAAAGGAT AAAGATCCTC CCATTCCTGT TGCCAAGATC 
GATGCAACCT CAGCGTCTGT GCTGGCCAGC AGGTTTGATG TGAGTGGCTA CCCCACCATC 
AAGATCCTTA AGAAGGGGCA GGCTGTAGAC TACGAGGGCT CCAGAACCCA GGAAGAAATT 
GTTGCCAAGG TCAGAGAAGT CTCCCAGCCC GACTGGACGC CTCCACCAGA AGTCACGCTT 
GTGTTGACCA AAGAGAACTT TGATGAAGTT GTGAATGATG CAGATATCAT TCTGGTGGAG 
TTTTATGCCC CATGTTTCCT CTTGGCAGGT GTGGACACTG CAAGAAACTT GCCCCCGAGT 
ATGAGAAGGC CGCCAAGGAG CTCAGCAAGC GTTCTCCTCC AATTCCCCTG GCAAAGGTCG 
ACGCCACCGC AGAAACAGAC CTGGCCAAGA GGAATCGTTG ATTACATGAT CGAGCAGTCC 
GGGCCTCCCT CCAAGGAGAT TCTGACCCTG AAGCAGGTCC AGGAGTTCCT GAAGGATGGA 
GACGATGTCA TCATCATCGG GGTCTTTAAG GGGGAGAGTG ACCCAGCCTA CCAGCAATAC 
CAGGATGCCG CTAACAACCT GAGAGAAGAT TACAAATTTC ACCACACTTT CAGCACAGAA 
ATAGCAAAGT TCTTGAAAGT CTCCCAGGGG CAGTTGGTTG TAATGCAGCC TGAGAAATTC 
CAGTCCAAGT ATGAGCCCCG GAGCCACATG ATGGACGTCC AGGGCTCCAC CCAGGACTCG 
GCCATCAAGG ACTTCGTGCT GAAGTACGCC CTGCCCCTGG TTGGCCACCG CAAGGTGTCA 
AACGATGCTA AGCGCTACAC CAGGCGCCCC CTGGTGGTCG TCTACTACAG TGTGGACTTC 
AGCTTTGATT ACAGAGCTGC AACTCAGTTT TGGCGGAGCA AAGTCCTAGA GGTGGCCAAG 
GACTTCCCTG AGTACACCTT TGCCATTGCG GACGAAGAGG ACTATGCTGG GGAGGTGAAG 
GACCTGGGGC TCAGCGAGAG TGGGGAGGAT GTCAATGCCG CCATCCTGGA CGAGAGTGGG 
AAGAAGTTCG CCATGGAGCC AGAGGAGTTT GACTCTGACA CCCTCCGCGA GTTTGTCACT 
GCTTTCAAAA AAGGAAAACT GAAGCCAGTC ATCAAATCCC AGCCAGTGCC CAAGAACAAC 
AAGGGACCCG TCAAGGTCGT GGTGGGAAAG ACCTTTGACT CCATTGTGAT GGACCCCAAG 
AAGGACGTCC TCATCGAGTT CTACGCGCCA TGGTGCGGGC ACTGCAAGCA GCTAGAGCCC 
GTGTACAACA GCCTGGCCAA GAAGTACAAG GGCCAAAAGG GCCTGGTCAT CGCCAAGATG 
GACGCCACTG CCAACGACGT CCCCAGCGAC CGCTATAAGG TGGAGGGCTT CCCCACCATC 
TACTTCGCCC CCAGTGGGGA CAAAAAGAAC CCAGTTAAAT TTGAGGGTGG AGACAGAGAT 
CTGGAGCATT TGAGCAAGTT TATAGAAGAA CATGCCACAA AACTGAGCAG GACCAAGGAA 
GAGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Protein disulfide isomerase a4 acts as a novel regulator of cancer growth through the procaspase pathway.
Oncogene36(39)5484-5496(2017 Sep)
Kuo TF,Chen TY,Jiang ST,Chen KW,Chiang YM,Hsu YJ,Liu YJ,Chen HM,Yokoyama KK,Tsai KC,Yeh HH,Chen YR,Yang MT,Yang CY,Yang WC

Interleukin-11 up-regulates endoplasmic reticulum stress induced target, PDIA4 in human first trimester placenta and in?vivo in mice.
Placenta5392-100(2017 May)
Winship AL,Sorby K,Correia J,Rainczuk A,Yap J,Dimitriadis E

A novel potential biomarker for metabolic syndrome in Chinese adults: Circulating protein disulfide isomerase family A, member 4.
PloS one12(6)e0179963(2017)
Chien CY,Hung YJ,Shieh YS,Hsieh CH,Lu CH,Lin FH,Su SC,Lee CH

Transient covalent interactions of newly synthesized thyroglobulin with oxidoreductases of the endoplasmic reticulum.
The Journal of biological chemistry289(16)11488-96(2014 Apr)
Di Jeso B,Morishita Y,Treglia AS,Lofrumento DD,Nicolardi G,Beguinot F,Kellogg AP,Arvan P

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PDIA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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