MED20 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED20
Entrez Gene ID	9477
Full Name	mediator complex subunit 20
Synonyms	PRO0213,SRB2,TRFP
General protein information	Preferred Names mediator complex subunit 20 Names mediator of RNA polymerase II transcription subunit 20 Trf (TATA binding protein-related factor)-proximal homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p21.1
Summary	This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2015].
Disorder MIM:	612915

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305455.1	NP_001292384.1	mediator of RNA polymerase II transcription subunit 20 isoform 2
NM_004275.4	NP_004266.2	mediator of RNA polymerase II transcription subunit 20 isoform 1
NM_004275.5	NP_004266.2	mediator of RNA polymerase II transcription subunit 20 isoform 1
NM_001305457.2	NP_001292386.1	mediator of RNA polymerase II transcription subunit 20 isoform 3
NM_001305457.1	NP_001292386.1	mediator of RNA polymerase II transcription subunit 20 isoform 3
NM_001305455.2	NP_001292384.1	mediator of RNA polymerase II transcription subunit 20 isoform 2
NM_001305456.1	NP_001292385.1	mediator of RNA polymerase II transcription subunit 20 isoform 2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MED20	NP_001006327.1
Anopheles gambiae (African malaria mosquito)	MED20_ANOGA	XP_315616.4
Caenorhabditis elegans (roundworm)	mdt-20	NP_500050.1
Pan troglodytes (chimpanzee)	MED20	NP_001233419.1
	MED20	XP_001085725.1
Danio rerio (zebrafish)	med20	NP_001003585.1
Xenopus tropicalis (tropical clawed frog)	med20	XP_002933024.2
Mus musculus (house mouse)	Med20	NP_064432.1
Rattus norvegicus (Norway rat)	Med20	NP_001013196.1
Homo sapiens (human)	MED20	NP_004266.2
Canis lupus familiaris (dog)	MED20	XP_005627389.1
Bos taurus (cattle)	MED20	NP_001096763.1
Drosophila melanogaster (fruit fly)	MED20	NP_001260223.1

Gene Ontology
Bibliography

Related articles in PubMed

All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids.
Shyam R, Vachali P, Gorusupudi A, Nelson K, Bernstein PS
Archives of biochemistry and biophysics63421-28(2017 Nov)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

MED20 mutation associated with infantile basal ganglia degeneration and brain atrophy.
Vodopiutz J, Schmook MT, Konstantopoulou V, Plecko B, Greber-Platzer S, Creus M, Seidl R, Janecke AR
European journal of pediatrics174(1)113-8(2015 Jan)

Gene expression profile of the whole Mediator complex in human osteosarcoma and normal osteoblasts.
Schiano C, Rienzo M, Casamassimi A, Napoli C
Medical oncology (Northwood, London, England)30(4)739(2013 Dec)

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
Molecular cell14(5)685-91(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our analyses revealed no apparent differences in protein expression profiles of SRBs in central and peripheral regions of human donor tissues, indicating that carotenoid-binding proteins rather than transporters are likely to mediate selective accumulation of carotenoids into the macula.
Title: All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids.

We identified the first and homozygous mutation (p.Gly114Ala) in the Mediator subunit 20 gene (MED20) in siblings presenting with infantile-onset spasticity and childhood-onset dystonia, progressive basal ganglia degeneration, and brain atrophy
Title: MED20 mutation associated with infantile basal ganglia degeneration and brain atrophy.

The following MED20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17078	NM_004275.5 Latest version!	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu62861	NM_001305456.1 Latest version!	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu62861	NM_001305455.2 Latest version!	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu62862	NM_001305457.2 Latest version!	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu17078	NM_004275.4	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu62862	NM_001305457.1	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu62861	NM_001305455.1	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED20 ( NM_004275.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004266.2 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17078
Clone ID Related Accession (Same CDS sequence)	NM_004275.4 , NM_004275.5
Accession Version	NM_004275.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA909832.1, BC012618.1 and BC040950.1. This sequence is a reference standard in the RefSeqGene project. On Mar 15, 2015 this sequence version replaced NM_004275.3. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC012618.1, BC019866.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTA CCAGTACTGT 
GACTTCCTGG TGAAGGTGGG CACGGTCACA ATGGGGCCCA GTGCCCGGGG CATCTCTGTG 
GAGGTGGAGT ATGGCCCCTG TGTGGTAGCT AGTGACTGCT GGAGTCTGCT GCTCGAGTTC 
CTACAGAGTT TTCTAGGCAG CCACACACCA GGGGCTCCCG CAGTGTTTGG GAACAGACAT 
GATGCGGTCT ACGGCCCAGC AGATACCATG GTCCAGTACA TGGAACTCTT CAACAAGATC 
CGCAAGCAGC AGCAGGTGCC GGTGGCTGGG ATTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004266.2
CDS	130..768
Translation	MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR

Target ORF information:

RefSeq Version	NM_004275.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004275.4

ORF Insert Sequence:

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTA CCAGTACTGT 
GACTTCCTGG TGAAGGTGGG CACGGTCACA ATGGGGCCCA GTGCCCGGGG CATCTCTGTG 
GAGGTGGAGT ATGGCCCCTG TGTGGTAGCT AGTGACTGCT GGAGTCTGCT GCTCGAGTTC 
CTACAGAGTT TTCTAGGCAG CCACACACCA GGGGCTCCCG CAGTGTTTGG GAACAGACAT 
GATGCGGTCT ACGGCCCAGC AGATACCATG GTCCAGTACA TGGAACTCTT CAACAAGATC 
CGCAAGCAGC AGCAGGTGCC GGTGGCTGGG ATTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED20 ( NM_004275.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004266.2 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17078
Clone ID Related Accession (Same CDS sequence)	NM_004275.4 , NM_004275.5
Accession Version	NM_004275.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012618.1 and BC040950.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004275.4. Transcript Variant: This variant (1) encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC019866.1, BC012618.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265350.9/ ENSP00000265350.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTA CCAGTACTGT 
GACTTCCTGG TGAAGGTGGG CACGGTCACA ATGGGGCCCA GTGCCCGGGG CATCTCTGTG 
GAGGTGGAGT ATGGCCCCTG TGTGGTAGCT AGTGACTGCT GGAGTCTGCT GCTCGAGTTC 
CTACAGAGTT TTCTAGGCAG CCACACACCA GGGGCTCCCG CAGTGTTTGG GAACAGACAT 
GATGCGGTCT ACGGCCCAGC AGATACCATG GTCCAGTACA TGGAACTCTT CAACAAGATC 
CGCAAGCAGC AGCAGGTGCC GGTGGCTGGG ATTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004266.2
CDS	122..760
Translation	MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR

Target ORF information:

RefSeq Version	NM_004275.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004275.5

ORF Insert Sequence:

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTA CCAGTACTGT 
GACTTCCTGG TGAAGGTGGG CACGGTCACA ATGGGGCCCA GTGCCCGGGG CATCTCTGTG 
GAGGTGGAGT ATGGCCCCTG TGTGGTAGCT AGTGACTGCT GGAGTCTGCT GCTCGAGTTC 
CTACAGAGTT TTCTAGGCAG CCACACACCA GGGGCTCCCG CAGTGTTTGG GAACAGACAT 
GATGCGGTCT ACGGCCCAGC AGATACCATG GTCCAGTACA TGGAACTCTT CAACAAGATC 
CGCAAGCAGC AGCAGGTGCC GGTGGCTGGG ATTCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED20 ( NM_001305455.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292384.1 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu62861
Clone ID Related Accession (Same CDS sequence)	NM_001305455.1 , NM_001305455.2 , NM_001305456.1
Accession Version	NM_001305455.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA909832.1, BC012618.1 and BC040950.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and uses a downstream in-frame start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Both variants 2 and 3 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.44445.1, SRR1803614.274496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGTGA TGCACAACTC AGAGTACCCA TTGAGCTGTT TCGCCCTCTT TGAGAATGGC 
CCTTGCCTTA TTGCTGACAC CAACTTTGAT GTGCTTATGG TGAAGCTCAA GGGCTTTTTC 
CAGAGTGCTA AGGCCAGCAA GATTGAGACC CGGGGCACCA GGTACCAGTA CTGTGACTTC 
CTGGTGAAGG TGGGCACGGT CACAATGGGG CCCAGTGCCC GGGGCATCTC TGTGGAGGTG 
GAGTATGGCC CCTGTGTGGT AGCTAGTGAC TGCTGGAGTC TGCTGCTCGA GTTCCTACAG 
AGTTTTCTAG GCAGCCACAC ACCAGGGGCT CCCGCAGTGT TTGGGAACAG ACATGATGCG 
GTCTACGGCC CAGCAGATAC CATGGTCCAG TACATGGAAC TCTTCAACAA GATCCGCAAG 
CAGCAGCAGG TGCCGGTGGC TGGGATTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292384.1
CDS	161..613
Translation	MYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR

Target ORF information:

RefSeq Version	NM_001305455.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305455.1

ORF Insert Sequence:

ATGTATGTGA TGCACAACTC AGAGTACCCA TTGAGCTGTT TCGCCCTCTT TGAGAATGGC 
CCTTGCCTTA TTGCTGACAC CAACTTTGAT GTGCTTATGG TGAAGCTCAA GGGCTTTTTC 
CAGAGTGCTA AGGCCAGCAA GATTGAGACC CGGGGCACCA GGTACCAGTA CTGTGACTTC 
CTGGTGAAGG TGGGCACGGT CACAATGGGG CCCAGTGCCC GGGGCATCTC TGTGGAGGTG 
GAGTATGGCC CCTGTGTGGT AGCTAGTGAC TGCTGGAGTC TGCTGCTCGA GTTCCTACAG 
AGTTTTCTAG GCAGCCACAC ACCAGGGGCT CCCGCAGTGT TTGGGAACAG ACATGATGCG 
GTCTACGGCC CAGCAGATAC CATGGTCCAG TACATGGAAC TCTTCAACAA GATCCGCAAG 
CAGCAGCAGG TGCCGGTGGC TGGGATTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED20 ( NM_001305455.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292384.1 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu62861
Clone ID Related Accession (Same CDS sequence)	NM_001305455.1 , NM_001305455.2 , NM_001305456.1
Accession Version	NM_001305455.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012618.1 and BC040950.1. On May 31, 2019 this sequence version replaced NM_001305455.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region and uses a downstream in-frame start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Both variants 2 and 3 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.164488.1, AK300573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGTGA TGCACAACTC AGAGTACCCA TTGAGCTGTT TCGCCCTCTT TGAGAATGGC 
CCTTGCCTTA TTGCTGACAC CAACTTTGAT GTGCTTATGG TGAAGCTCAA GGGCTTTTTC 
CAGAGTGCTA AGGCCAGCAA GATTGAGACC CGGGGCACCA GGTACCAGTA CTGTGACTTC 
CTGGTGAAGG TGGGCACGGT CACAATGGGG CCCAGTGCCC GGGGCATCTC TGTGGAGGTG 
GAGTATGGCC CCTGTGTGGT AGCTAGTGAC TGCTGGAGTC TGCTGCTCGA GTTCCTACAG 
AGTTTTCTAG GCAGCCACAC ACCAGGGGCT CCCGCAGTGT TTGGGAACAG ACATGATGCG 
GTCTACGGCC CAGCAGATAC CATGGTCCAG TACATGGAAC TCTTCAACAA GATCCGCAAG 
CAGCAGCAGG TGCCGGTGGC TGGGATTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292384.1
CDS	153..605
Translation	MYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR

Target ORF information:

RefSeq Version	NM_001305455.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305455.2

ORF Insert Sequence:

ATGTATGTGA TGCACAACTC AGAGTACCCA TTGAGCTGTT TCGCCCTCTT TGAGAATGGC 
CCTTGCCTTA TTGCTGACAC CAACTTTGAT GTGCTTATGG TGAAGCTCAA GGGCTTTTTC 
CAGAGTGCTA AGGCCAGCAA GATTGAGACC CGGGGCACCA GGTACCAGTA CTGTGACTTC 
CTGGTGAAGG TGGGCACGGT CACAATGGGG CCCAGTGCCC GGGGCATCTC TGTGGAGGTG 
GAGTATGGCC CCTGTGTGGT AGCTAGTGAC TGCTGGAGTC TGCTGCTCGA GTTCCTACAG 
AGTTTTCTAG GCAGCCACAC ACCAGGGGCT CCCGCAGTGT TTGGGAACAG ACATGATGCG 
GTCTACGGCC CAGCAGATAC CATGGTCCAG TACATGGAAC TCTTCAACAA GATCCGCAAG 
CAGCAGCAGG TGCCGGTGGC TGGGATTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED20 ( NM_001305456.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292385.1 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu62861
Clone ID Related Accession (Same CDS sequence)	NM_001305455.1 , NM_001305455.2 , NM_001305456.1
Accession Version	NM_001305456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK097385.1, BC012618.1, AL160163.24 and BC040950.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon and contains an alternate exon in the coding region, and uses a downstream in-frame start codon compared to variant 1. It encodes isoform 2 which has a shorter N-terminus compared to isoform 1. Both variants 2 and 3 encode the same isoform. ##Evidence-Data-START## Transcript exon combination :: AK097385.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTATGTGA TGCACAACTC AGAGTACCCA TTGAGCTGTT TCGCCCTCTT TGAGAATGGC 
CCTTGCCTTA TTGCTGACAC CAACTTTGAT GTGCTTATGG TGAAGCTCAA GGGCTTTTTC 
CAGAGTGCTA AGGCCAGCAA GATTGAGACC CGGGGCACCA GGTACCAGTA CTGTGACTTC 
CTGGTGAAGG TGGGCACGGT CACAATGGGG CCCAGTGCCC GGGGCATCTC TGTGGAGGTG 
GAGTATGGCC CCTGTGTGGT AGCTAGTGAC TGCTGGAGTC TGCTGCTCGA GTTCCTACAG 
AGTTTTCTAG GCAGCCACAC ACCAGGGGCT CCCGCAGTGT TTGGGAACAG ACATGATGCG 
GTCTACGGCC CAGCAGATAC CATGGTCCAG TACATGGAAC TCTTCAACAA GATCCGCAAG 
CAGCAGCAGG TGCCGGTGGC TGGGATTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292385.1
CDS	433..885
Translation	MYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRYQYCDFLVKVGTVTMGPSARGISVEVEYGPCVVASDCWSLLLEFLQSFLGSHTPGAPAVFGNRHDAVYGPADTMVQYMELFNKIRKQQQVPVAGIR

Target ORF information:

RefSeq Version	NM_001305456.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305456.1

ORF Insert Sequence:

ATGTATGTGA TGCACAACTC AGAGTACCCA TTGAGCTGTT TCGCCCTCTT TGAGAATGGC 
CCTTGCCTTA TTGCTGACAC CAACTTTGAT GTGCTTATGG TGAAGCTCAA GGGCTTTTTC 
CAGAGTGCTA AGGCCAGCAA GATTGAGACC CGGGGCACCA GGTACCAGTA CTGTGACTTC 
CTGGTGAAGG TGGGCACGGT CACAATGGGG CCCAGTGCCC GGGGCATCTC TGTGGAGGTG 
GAGTATGGCC CCTGTGTGGT AGCTAGTGAC TGCTGGAGTC TGCTGCTCGA GTTCCTACAG 
AGTTTTCTAG GCAGCCACAC ACCAGGGGCT CCCGCAGTGT TTGGGAACAG ACATGATGCG 
GTCTACGGCC CAGCAGATAC CATGGTCCAG TACATGGAAC TCTTCAACAA GATCCGCAAG 
CAGCAGCAGG TGCCGGTGGC TGGGATTCGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED20 ( NM_001305457.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292386.1 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu62862
Clone ID Related Accession (Same CDS sequence)	NM_001305457.2 , NM_001305457.1
Accession Version	NM_001305457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012618.1 and BC040950.1. On May 31, 2019 this sequence version replaced NM_001305457.1. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.700612.1, DRR138519.293740.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTG GAGTATGGCC 
CCTGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292386.1
CDS	122..493
Translation	MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRWSMAPVW

Target ORF information:

RefSeq Version	NM_001305457.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305457.2

ORF Insert Sequence:

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTG GAGTATGGCC 
CCTGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED20 ( NM_001305457.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001292386.1 Homo sapiens mediator complex subunit 20 (MED20), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu62862
Clone ID Related Accession (Same CDS sequence)	NM_001305457.2 , NM_001305457.1
Accession Version	NM_001305457.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 20 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA909832.1, BC012618.1 and BC040950.1. Transcript Variant: This variant (4) uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a shorter and distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK293412.1, SRR1803617.305128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTG GAGTATGGCC 
CCTGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292386.1
CDS	130..501
Translation	MGVTCVSQMPVAEGKSVQQTVELLTRKLEMLGAEKQGTFCVDCETYHTAASTLGSQGQTGKLMYVMHNSEYPLSCFALFENGPCLIADTNFDVLMVKLKGFFQSAKASKIETRGTRWSMAPVW

Target ORF information:

RefSeq Version	NM_001305457.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 20 (MED20), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305457.1

ORF Insert Sequence:

ATGGGAGTGA CTTGTGTGTC CCAGATGCCT GTGGCCGAGG GCAAGAGTGT TCAGCAAACC 
GTAGAGCTCC TTACCCGGAA ATTGGAGATG CTTGGGGCAG AGAAGCAAGG AACATTTTGT 
GTGGACTGTG AGACTTACCA TACGGCCGCC TCTACCCTTG GCAGCCAAGG TCAGACCGGG 
AAGCTGATGT ATGTGATGCA CAACTCAGAG TACCCATTGA GCTGTTTCGC CCTCTTTGAG 
AATGGCCCTT GCCTTATTGC TGACACCAAC TTTGATGTGC TTATGGTGAA GCTCAAGGGC 
TTTTTCCAGA GTGCTAAGGC CAGCAAGATT GAGACCCGGG GCACCAGGTG GAGTATGGCC 
CCTGTGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

All three human scavenger receptor class B proteins can bind and transport all three macular xanthophyll carotenoids.
Archives of biochemistry and biophysics63421-28(2017 Nov)
Shyam R,Vachali P,Gorusupudi A,Nelson K,Bernstein PS

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

MED20 mutation associated with infantile basal ganglia degeneration and brain atrophy.
European journal of pediatrics174(1)113-8(2015 Jan)
Vodopiutz J,Schmook MT,Konstantopoulou V,Plecko B,Greber-Platzer S,Creus M,Seidl R,Janecke AR

Gene expression profile of the whole Mediator complex in human osteosarcoma and normal osteoblasts.
Medical oncology (Northwood, London, England)30(4)739(2013 Dec)
Schiano C,Rienzo M,Casamassimi A,Napoli C

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Molecular cell14(5)685-91(2004 Jun)
Sato S,Tomomori-Sato C,Parmely TJ,Florens L,Zybailov B,Swanson SK,Banks CA,Jin J,Cai Y,Washburn MP,Conaway JW,Conaway RC

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MED20 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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