ITM2B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITM2B
Entrez Gene ID	9445
Full Name	integral membrane protein 2B
Synonyms	ABRI,BRI,BRI2,BRICD2B,E25B,E3-16,FBD,RDGCA,imBRI2
General protein information	Preferred Names integral membrane protein 2B Names integral membrane protein 2B ABri/ADan amyloid peptide BRICHOS domain containing 2B immature BRI2 transmembrane protein BRI
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q14.2
Summary	Amyloid precursor proteins are processed by beta-secretase and gamma-secretase to produce beta-amyloid peptides which form the characteristic plaques of Alzheimer disease. This gene encodes a transmembrane protein which is processed at the C-terminus by furin or furin-like proteases to produce a small secreted peptide which inhibits the deposition of beta-amyloid. Mutations which result in extension of the C-terminal end of the encoded protein, thereby increasing the size of the secreted peptide, are associated with two neurogenerative diseases, familial British dementia and familial Danish dementia. [provided by RefSeq, Oct 2009].
Disorder MIM:	603904
Disorder Html:	Dementia, familial British, 176500 (3); Dementia, familial Danish, 117300 (3); ?Retinal dystrophy with inner retinal dysfunction and ganglion cell abnormalities, 616079 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_021999.4	NP_068839.1	integral membrane protein 2B
NM_021999.5	NP_068839.1	integral membrane protein 2B

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Itm2b	NP_032436.1
Danio rerio (zebrafish)	itm2ba	NP_955940.1
	ITM2B	XP_002800791.1
Bos taurus (cattle)	ITM2B	NP_001030265.1
Rattus norvegicus (Norway rat)	Itm2b	NP_001006964.1
Canis lupus familiaris (dog)	ITM2B	XP_005633935.1
Gallus gallus (chicken)	ITM2B	NP_990416.1
Homo sapiens (human)	ITM2B	NP_068839.1
Pan troglodytes (chimpanzee)	ITM2B	NP_001092017.1
Xenopus tropicalis (tropical clawed frog)	itm2b	NP_989241.1

Gene Ontology
Bibliography

Related articles in PubMed

Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state.
Chen G, Abelein A, Nilsson HE, Leppert A, Andrade-Talavera Y, Tambaro S, Hemmingsson L, Roshan F, Landreh M, Biverst?l H, Koeck PJB, Presto J, Hebert H, Fisahn A, Johansson J
Nature communications8(1)2081(2017 Dec)

BRI2 Processing and Its Neuritogenic Role Are Modulated by Protein Phosphatase 1 Complexing.
Martins F, Serrano JB, M?ller T, da Cruz E Silva OAB, Rebelo S
Journal of cellular biochemistry118(9)2752-2763(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

by modulating BRI2 processing using an ADAM10 inhibitor, a dual role for BRI2 in neurite outgrowth is suggested: phosphorylated full-length BRI2 appears to be important for the formation of neuritic processes, and BRI2 NTF promotes neurite elongation.
Title: BRI2 Processing and Its Neuritogenic Role Are Modulated by Protein Phosphatase 1 Complexing.

Familial British dementia (FBD) and familial Danish dementia (FDD) are caused by mutations in the BRI2 gene.
Title: Amyloid and intracellular accumulation of BRI

when BRI2 is phosphorylated a significant increase in neuronal outgrowth and differentiation is evident
Title: BRI2 and BRI3 are functionally distinct phosphoproteins.

familial Danish dementia mutation and pE posttranslational modification are primary elements driving intact ADan into an amyloidogenic/neurotoxic pathway while truncations at the C-terminus eliminate the pro-amyloidogenic characteristics of the molecule
Title: Oxidative stress and mitochondria-mediated cell death mechanisms triggered by the familial Danish dementia ADan amyloid.

Data depict roles for Bri2 and apolipoprotein E4 (ApoE4) proteins in a long-term memory regulation pathway. A single mutation in one Itm2b/Bri2 allele can affect both long-term and working memory, while ApoE4 seems to regulate short-term/working memory.
Title: Interaction of ApoE3 and ApoE4 isoforms with an ITM2b/BRI2 mutation linked to the Alzheimer disease-like Danish dementia: Effects on learning and memory.

The following ITM2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITM2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27275	NM_021999.5 Latest version!	Homo sapiens integral membrane protein 2B (ITM2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu27275	NM_021999.4	Homo sapiens integral membrane protein 2B (ITM2B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITM2B ( NM_021999.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_068839.1 Homo sapiens integral membrane protein 2B (ITM2B), mRNA.

Price & Availability↑

CloneID	OHu27275
Clone ID Related Accession (Same CDS sequence)	NM_021999.4 , NM_021999.5
Accession Version	NM_021999.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	integral membrane protein 2B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA087198.1, AK130048.1 and AF152462.1. This sequence is a reference standard in the RefSeqGene project. On Jan 24, 2009 this sequence version replaced NM_021999.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF152462.1, SRR1660809.109424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGG TGACGTTCAA CTCCGCTCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCGGCG AGGAGGCGCT CATCATCCCC CCCGACGCCG TCGCGGTGGA CTGCAAGGAC 
CCAGATGATG TGGTACCAGT TGGCCAAAGA AGAGCCTGGT GTTGGTGCAT GTGCTTTGGA 
CTAGCATTTA TGCTTGCAGG TGTTATTCTA GGAGGAGCAT ACTTGTACAA ATATTTTGCA 
CTTCAACCAG ATGACGTGTA CTACTGTGGA ATAAAGTACA TCAAAGATGA TGTCATCTTA 
AATGAGCCCT CTGCAGATGC CCCAGCTGCT CTCTACCAGA CAATTGAAGA AAATATTAAA 
ATCTTTGAAG AAGAAGAAGT TGAATTTATC AGTGTGCCTG TCCCAGAGTT TGCAGATAGT 
GATCCTGCCA ACATTGTTCA TGACTTTAAC AAGAAACTTA CAGCCTATTT AGATCTTAAC 
CTGGATAAGT GCTATGTGAT CCCTCTGAAC ACTTCCATTG TTATGCCACC CAGAAACCTA 
CTGGAGTTAC TTATTAACAT CAAGGCTGGA ACCTATTTGC CTCAGTCCTA TCTGATTCAT 
GAGCACATGG TTATTACTGA TCGCATTGAA AACATTGATC ACCTGGGTTT CTTTATTTAT 
CGACTGTGTC ATGACAAGGA AACTTACAAA CTGCAACGCA GAGAAACTAT TAAAGGTATT 
CAGAAACGTG AAGCCAGCAA TTGTTTCGCA ATTCGGCATT TTGAAAACAA ATTTGCCGTG 
GAAACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068839.1
CDS	224..1024
Translation	MVKVTFNSALAQKEAKKDEPKSGEEALIIPPDAVAVDCKDPDDVVPVGQRRAWCWCMCFGLAFMLAGVILGGAYLYKYFALQPDDVYYCGIKYIKDDVILNEPSADAPAALYQTIEENIKIFEEEEVEFISVPVPEFADSDPANIVHDFNKKLTAYLDLNLDKCYVIPLNTSIVMPPRNLLELLINIKAGTYLPQSYLIHEHMVITDRIENIDHLGFFIYRLCHDKETYKLQRRETIKGIQKREASNCFAIRHFENKFAVETLICS

Target ORF information:

RefSeq Version	NM_021999.4
Organism	Homo sapiens(human)
Definition	Homo sapiens integral membrane protein 2B (ITM2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021999.4

ORF Insert Sequence:

ATGGTGAAGG TGACGTTCAA CTCCGCTCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCGGCG AGGAGGCGCT CATCATCCCC CCCGACGCCG TCGCGGTGGA CTGCAAGGAC 
CCAGATGATG TGGTACCAGT TGGCCAAAGA AGAGCCTGGT GTTGGTGCAT GTGCTTTGGA 
CTAGCATTTA TGCTTGCAGG TGTTATTCTA GGAGGAGCAT ACTTGTACAA ATATTTTGCA 
CTTCAACCAG ATGACGTGTA CTACTGTGGA ATAAAGTACA TCAAAGATGA TGTCATCTTA 
AATGAGCCCT CTGCAGATGC CCCAGCTGCT CTCTACCAGA CAATTGAAGA AAATATTAAA 
ATCTTTGAAG AAGAAGAAGT TGAATTTATC AGTGTGCCTG TCCCAGAGTT TGCAGATAGT 
GATCCTGCCA ACATTGTTCA TGACTTTAAC AAGAAACTTA CAGCCTATTT AGATCTTAAC 
CTGGATAAGT GCTATGTGAT CCCTCTGAAC ACTTCCATTG TTATGCCACC CAGAAACCTA 
CTGGAGTTAC TTATTAACAT CAAGGCTGGA ACCTATTTGC CTCAGTCCTA TCTGATTCAT 
GAGCACATGG TTATTACTGA TCGCATTGAA AACATTGATC ACCTGGGTTT CTTTATTTAT 
CGACTGTGTC ATGACAAGGA AACTTACAAA CTGCAACGCA GAGAAACTAT TAAAGGTATT 
CAGAAACGTG AAGCCAGCAA TTGTTTCGCA ATTCGGCATT TTGAAAACAA ATTTGCCGTG 
GAAACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITM2B ( NM_021999.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_068839.1 Homo sapiens integral membrane protein 2B (ITM2B), mRNA.

Price & Availability↑

CloneID	OHu27275
Clone ID Related Accession (Same CDS sequence)	NM_021999.4 , NM_021999.5
Accession Version	NM_021999.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	integral membrane protein 2B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA087198.1, AK130048.1 and AL139322.13. This sequence is a reference standard in the RefSeqGene project. On Nov 14, 2018 this sequence version replaced NM_021999.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK130048.1, AK000503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000647800.2/ ENSP00000497221.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGG TGACGTTCAA CTCCGCTCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCGGCG AGGAGGCGCT CATCATCCCC CCCGACGCCG TCGCGGTGGA CTGCAAGGAC 
CCAGATGATG TGGTACCAGT TGGCCAAAGA AGAGCCTGGT GTTGGTGCAT GTGCTTTGGA 
CTAGCATTTA TGCTTGCAGG TGTTATTCTA GGAGGAGCAT ACTTGTACAA ATATTTTGCA 
CTTCAACCAG ATGACGTGTA CTACTGTGGA ATAAAGTACA TCAAAGATGA TGTCATCTTA 
AATGAGCCCT CTGCAGATGC CCCAGCTGCT CTCTACCAGA CAATTGAAGA AAATATTAAA 
ATCTTTGAAG AAGAAGAAGT TGAATTTATC AGTGTGCCTG TCCCAGAGTT TGCAGATAGT 
GATCCTGCCA ACATTGTTCA TGACTTTAAC AAGAAACTTA CAGCCTATTT AGATCTTAAC 
CTGGATAAGT GCTATGTGAT CCCTCTGAAC ACTTCCATTG TTATGCCACC CAGAAACCTA 
CTGGAGTTAC TTATTAACAT CAAGGCTGGA ACCTATTTGC CTCAGTCCTA TCTGATTCAT 
GAGCACATGG TTATTACTGA TCGCATTGAA AACATTGATC ACCTGGGTTT CTTTATTTAT 
CGACTGTGTC ATGACAAGGA AACTTACAAA CTGCAACGCA GAGAAACTAT TAAAGGTATT 
CAGAAACGTG AAGCCAGCAA TTGTTTCGCA ATTCGGCATT TTGAAAACAA ATTTGCCGTG 
GAAACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068839.1
CDS	156..956
Translation	MVKVTFNSALAQKEAKKDEPKSGEEALIIPPDAVAVDCKDPDDVVPVGQRRAWCWCMCFGLAFMLAGVILGGAYLYKYFALQPDDVYYCGIKYIKDDVILNEPSADAPAALYQTIEENIKIFEEEEVEFISVPVPEFADSDPANIVHDFNKKLTAYLDLNLDKCYVIPLNTSIVMPPRNLLELLINIKAGTYLPQSYLIHEHMVITDRIENIDHLGFFIYRLCHDKETYKLQRRETIKGIQKREASNCFAIRHFENKFAVETLICS

Target ORF information:

RefSeq Version	NM_021999.5
Organism	Homo sapiens(human)
Definition	Homo sapiens integral membrane protein 2B (ITM2B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021999.5

ORF Insert Sequence:

ATGGTGAAGG TGACGTTCAA CTCCGCTCTG GCCCAGAAGG AGGCCAAGAA GGACGAGCCC 
AAGAGCGGCG AGGAGGCGCT CATCATCCCC CCCGACGCCG TCGCGGTGGA CTGCAAGGAC 
CCAGATGATG TGGTACCAGT TGGCCAAAGA AGAGCCTGGT GTTGGTGCAT GTGCTTTGGA 
CTAGCATTTA TGCTTGCAGG TGTTATTCTA GGAGGAGCAT ACTTGTACAA ATATTTTGCA 
CTTCAACCAG ATGACGTGTA CTACTGTGGA ATAAAGTACA TCAAAGATGA TGTCATCTTA 
AATGAGCCCT CTGCAGATGC CCCAGCTGCT CTCTACCAGA CAATTGAAGA AAATATTAAA 
ATCTTTGAAG AAGAAGAAGT TGAATTTATC AGTGTGCCTG TCCCAGAGTT TGCAGATAGT 
GATCCTGCCA ACATTGTTCA TGACTTTAAC AAGAAACTTA CAGCCTATTT AGATCTTAAC 
CTGGATAAGT GCTATGTGAT CCCTCTGAAC ACTTCCATTG TTATGCCACC CAGAAACCTA 
CTGGAGTTAC TTATTAACAT CAAGGCTGGA ACCTATTTGC CTCAGTCCTA TCTGATTCAT 
GAGCACATGG TTATTACTGA TCGCATTGAA AACATTGATC ACCTGGGTTT CTTTATTTAT 
CGACTGTGTC ATGACAAGGA AACTTACAAA CTGCAACGCA GAGAAACTAT TAAAGGTATT 
CAGAAACGTG AAGCCAGCAA TTGTTTCGCA ATTCGGCATT TTGAAAACAA ATTTGCCGTG 
GAAACTTTAA TTTGTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bri2 BRICHOS client specificity and chaperone activity are governed by assembly state.
Nature communications8(1)2081(2017 Dec)
Chen G,Abelein A,Nilsson HE,Leppert A,Andrade-Talavera Y,Tambaro S,Hemmingsson L,Roshan F,Landreh M,Biverst?l H,Koeck PJB,Presto J,Hebert H,Fisahn A,Johansson J

BRI2 Processing and Its Neuritogenic Role Are Modulated by Protein Phosphatase 1 Complexing.
Journal of cellular biochemistry118(9)2752-2763(2017 Sep)
Martins F,Serrano JB,M?ller T,da Cruz E Silva OAB,Rebelo S

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ITM2B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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