MED27 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_004269.4	NP_004260.2	mediator of RNA polymerase II transcription subunit 27 isoform 1
NM_004269.3	NP_004260.2	mediator of RNA polymerase II transcription subunit 27 isoform 1
NM_001253882.1	NP_001240811.1	mediator of RNA polymerase II transcription subunit 27 isoform 3
XM_017015330.2	XP_016870819.1	mediator of RNA polymerase II transcription subunit 27 isoform X2
XM_005272236.3	XP_005272293.1	mediator of RNA polymerase II transcription subunit 27 isoform X3
NM_001253881.1	NP_001240810.1	mediator of RNA polymerase II transcription subunit 27 isoform 2
NM_001253881.2	NP_001240810.1	mediator of RNA polymerase II transcription subunit 27 isoform 2
NM_001253882.2	NP_001240811.1	mediator of RNA polymerase II transcription subunit 27 isoform 3
XM_017015330.1	XP_016870819.1	mediator of RNA polymerase II transcription subunit 27 isoform X2
XM_017015329.1	XP_016870818.1	mediator of RNA polymerase II transcription subunit 27 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04647	NM_004269.4 Latest version!	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu02558	NM_001253882.2 Latest version!	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu55977	NM_001253881.2 Latest version!	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu43602	XM_005272236.3 Latest version!	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu99020	XM_017015329.1 Latest version!	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu99021	XM_017015330.2 Latest version!	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu04647	NM_004269.3	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu55977	NM_001253881.1	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu02558	NM_001253882.1	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu99021	XM_017015330.1	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

MED27 ( NM_004269.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004260.2 Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04647
Clone ID Related Accession (Same CDS sequence)	NM_004269.4 , NM_004269.3
Accession Version	NM_004269.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP255072.1 and BC064608.1. On Nov 22, 2018 this sequence version replaced NM_004269.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.373773.1, SRR5189655.127456.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000292035.10/ ENSP00000292035.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GCTTGATATA TGGTCCAAAT CCAACTATCA AGTATTCCAG 
AAGGTGACAG ACCATGCCAC CACTGCCCTG CTCCACTATC AGCTGCCCCA GATGCCGGAT 
GTCGTGGTCC GATCCTTCAT GACCTGGTTA AGAAGTTACA TAAAGCTGTT CCAGGCCCCG 
TGCCAGCGCT GCGGGAAGTT TCTGCAGGAC GGCCTTCCCC CGACATGGAG GGATTTCCGA 
ACCCTCGAAG CCTTCCATGA CACCTGCCGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004260.2
CDS	24..959
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_004269.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004269.4

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GCTTGATATA TGGTCCAAAT CCAACTATCA AGTATTCCAG 
AAGGTGACAG ACCATGCCAC CACTGCCCTG CTCCACTATC AGCTGCCCCA GATGCCGGAT 
GTCGTGGTCC GATCCTTCAT GACCTGGTTA AGAAGTTACA TAAAGCTGTT CCAGGCCCCG 
TGCCAGCGCT GCGGGAAGTT TCTGCAGGAC GGCCTTCCCC CGACATGGAG GGATTTCCGA 
ACCCTCGAAG CCTTCCATGA CACCTGCCGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( NM_004269.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004260.2 Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04647
Clone ID Related Accession (Same CDS sequence)	NM_004269.4 , NM_004269.3
Accession Version	NM_004269.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP255072.1, BC064608.1 and AI033804.1. On Dec 9, 2011 this sequence version replaced NM_004269.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1163655.459602.1, SRR1660805.326581.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GCTTGATATA TGGTCCAAAT CCAACTATCA AGTATTCCAG 
AAGGTGACAG ACCATGCCAC CACTGCCCTG CTCCACTATC AGCTGCCCCA GATGCCGGAT 
GTCGTGGTCC GATCCTTCAT GACCTGGTTA AGAAGTTACA TAAAGCTGTT CCAGGCCCCG 
TGCCAGCGCT GCGGGAAGTT TCTGCAGGAC GGCCTTCCCC CGACATGGAG GGATTTCCGA 
ACCCTCGAAG CCTTCCATGA CACCTGCCGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004260.2
CDS	44..979
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_004269.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004269.3

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GCTTGATATA TGGTCCAAAT CCAACTATCA AGTATTCCAG 
AAGGTGACAG ACCATGCCAC CACTGCCCTG CTCCACTATC AGCTGCCCCA GATGCCGGAT 
GTCGTGGTCC GATCCTTCAT GACCTGGTTA AGAAGTTACA TAAAGCTGTT CCAGGCCCCG 
TGCCAGCGCT GCGGGAAGTT TCTGCAGGAC GGCCTTCCCC CGACATGGAG GGATTTCCGA 
ACCCTCGAAG CCTTCCATGA CACCTGCCGG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( NM_001253882.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240811.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02558
Clone ID Related Accession (Same CDS sequence)	NM_001253882.1 , NM_001253882.2
Accession Version	NM_001253882.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-08
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP255072.1, CD388680.1 and BU069864.1. Transcript Variant: This variant (3) uses an alternate terminal exon and lacks a large portion of the 3' coding region, compared to variant 1. The encoded isoform (3) is significantly shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.101455.1, SRR1163657.338563.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGAA AGAGCAACTT 
TCTATTCCAA GAATATTCCA TTGGAAAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240811.1
CDS	44..436
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKKEQLSIPRIFHWKV

Target ORF information:

RefSeq Version	NM_001253882.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253882.1

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGAA AGAGCAACTT 
TCTATTCCAA GAATATTCCA TTGGAAAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( NM_001253882.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240811.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu02558
Clone ID Related Accession (Same CDS sequence)	NM_001253882.1 , NM_001253882.2
Accession Version	NM_001253882.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP255072.1, CD388680.1 and BU069864.1. On May 31, 2019 this sequence version replaced NM_001253882.1. Transcript Variant: This variant (3) uses an alternate terminal exon and lacks a large portion of the 3' coding region, compared to variant 1. The encoded isoform (3) is significantly shorter and has a distinct C-terminus, compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.101455.1, CK300803.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA2144333 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGAA AGAGCAACTT 
TCTATTCCAA GAATATTCCA TTGGAAAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240811.1
CDS	24..416
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKKEQLSIPRIFHWKV

Target ORF information:

RefSeq Version	NM_001253882.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253882.2

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGAA AGAGCAACTT 
TCTATTCCAA GAATATTCCA TTGGAAAGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( NM_001253881.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240810.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55977
Clone ID Related Accession (Same CDS sequence)	NM_001253881.1 , NM_001253881.2
Accession Version	NM_001253881.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-08
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP255072.1, BC002878.1 and AI033804.1. Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. the encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC002878.1, SRR1163658.536045.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGCTTGATA TATGGTCCAA ATCCAACTAT 
CAAGTATTCC AGAAGGTGAC AGACCATGCC ACCACTGCCC TGCTCCACTA TCAGCTGCCC 
CAGATGCCGG ATGTCGTGGT CCGATCCTTC ATGACCTGGT TAAGAAGTTA CATAAAGCTG 
TTCCAGGCCC CGTGCCAGCG CTGCGGGAAG TTTCTGCAGG ACGGCCTTCC CCCGACATGG 
AGGGATTTCC GAACCCTCGA AGCCTTCCAT GACACCTGCC GGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240810.1
CDS	44..871
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_001253881.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253881.1

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGCTTGATA TATGGTCCAA ATCCAACTAT 
CAAGTATTCC AGAAGGTGAC AGACCATGCC ACCACTGCCC TGCTCCACTA TCAGCTGCCC 
CAGATGCCGG ATGTCGTGGT CCGATCCTTC ATGACCTGGT TAAGAAGTTA CATAAAGCTG 
TTCCAGGCCC CGTGCCAGCG CTGCGGGAAG TTTCTGCAGG ACGGCCTTCC CCCGACATGG 
AGGGATTTCC GAACCCTCGA AGCCTTCCAT GACACCTGCC GGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( NM_001253881.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001240810.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55977
Clone ID Related Accession (Same CDS sequence)	NM_001253881.1 , NM_001253881.2
Accession Version	NM_001253881.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP255072.1 and BC002878.1. On May 31, 2019 this sequence version replaced NM_001253881.1. Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. the encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: GQ900957.1, BC002878.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGCTTGATA TATGGTCCAA ATCCAACTAT 
CAAGTATTCC AGAAGGTGAC AGACCATGCC ACCACTGCCC TGCTCCACTA TCAGCTGCCC 
CAGATGCCGG ATGTCGTGGT CCGATCCTTC ATGACCTGGT TAAGAAGTTA CATAAAGCTG 
TTCCAGGCCC CGTGCCAGCG CTGCGGGAAG TTTCTGCAGG ACGGCCTTCC CCCGACATGG 
AGGGATTTCC GAACCCTCGA AGCCTTCCAT GACACCTGCC GGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001240810.1
CDS	24..851
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	NM_001253881.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001253881.2

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGCTTGATA TATGGTCCAA ATCCAACTAT 
CAAGTATTCC AGAAGGTGAC AGACCATGCC ACCACTGCCC TGCTCCACTA TCAGCTGCCC 
CAGATGCCGG ATGTCGTGGT CCGATCCTTC ATGACCTGGT TAAGAAGTTA CATAAAGCTG 
TTCCAGGCCC CGTGCCAGCG CTGCGGGAAG TTTCTGCAGG ACGGCCTTCC CCCGACATGG 
AGGGATTTCC GAACCCTCGA AGCCTTCCAT GACACCTGCC GGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( XM_005272236.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272293.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant X3, mRNA.

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CloneID	OHu43602
Clone ID Related Accession (Same CDS sequence)	XM_005272236.3
Accession Version	XM_005272236.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GCTTGATATA TGGTCCAAAT CCAACTATCA AGTATTCCAG 
AAGGCCTGTT TTTTCCAAGT CTTGGATGCT GAGCAAAAAA ACAACTTTGT TGTTGAAAAA 
TGTAAAACAA AAAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272293.1
CDS	23..823
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKLDIWSKSNYQVFQKACFFQVLDAEQKNNFVVEKCKTKKY

Target ORF information:

RefSeq Version	XM_005272236.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272236.3

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GCTTGATATA TGGTCCAAAT CCAACTATCA AGTATTCCAG 
AAGGCCTGTT TTTTCCAAGT CTTGGATGCT GAGCAAAAAA ACAACTTTGT TGTTGAAAAA 
TGTAAAACAA AAAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( XM_017015329.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870818.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu99020
Clone ID Related Accession (Same CDS sequence)	XM_017015329.1
Accession Version	XM_017015329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GTTCTTCCTG TGTGTCAAGA GGTCTTTTGA TGTCTTGGTG 
TTTGCCCTGA GAACAGACGT GTCTGTGCTG AAGCAATTGG TCATCTGCAA GCTTGATATA 
TGGTCCAAAT CCAACTATCA AGTATTCCAG AAGGTGACAG ACCATGCCAC CACTGCCCTG 
CTCCACTATC AGCTGCCCCA GATGCCGGAT GTCGTGGTCC GATCCTTCAT GACCTGGTTA 
AGAAGTTACA TAAAGCTGTT CCAGGCCCCG TGCCAGCGCT GCGGGAAGTT TCTGCAGGAC 
GGCCTTCCCC CGACATGGAG GGATTTCCGA ACCCTCGAAG CCTTCCATGA CACCTGCCGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870818.1
CDS	23..1048
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKFFLCVKRSFDVLVFALRTDVSVLKQLVICKLDIWSKSNYQVFQKVTDHATTALLHYQLPQMPDVVVRSFMTWLRSYIKLFQAPCQRCGKFLQDGLPPTWRDFRTLEAFHDTCRQ

Target ORF information:

RefSeq Version	XM_017015329.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015329.1

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GTTCTTCCTG TGTGTCAAGA GGTCTTTTGA TGTCTTGGTG 
TTTGCCCTGA GAACAGACGT GTCTGTGCTG AAGCAATTGG TCATCTGCAA GCTTGATATA 
TGGTCCAAAT CCAACTATCA AGTATTCCAG AAGGTGACAG ACCATGCCAC CACTGCCCTG 
CTCCACTATC AGCTGCCCCA GATGCCGGAT GTCGTGGTCC GATCCTTCAT GACCTGGTTA 
AGAAGTTACA TAAAGCTGTT CCAGGCCCCG TGCCAGCGCT GCGGGAAGTT TCTGCAGGAC 
GGCCTTCCCC CGACATGGAG GGATTTCCGA ACCCTCGAAG CCTTCCATGA CACCTGCCGG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( XM_017015330.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870819.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99021
Clone ID Related Accession (Same CDS sequence)	XM_017015330.2 , XM_017015330.1
Accession Version	XM_017015330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017015330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GTTCTTCCTG TGTGTCAAGA GGTCTTTTGA TGTCTTGGTG 
TTTGCCCTGA GAACAGACGT GTCTGTGCTG AAGCAATTGG TCATCTGCAA GCTTGATATA 
TGGTCCAAAT CCAACTATCA AGTATTCCAG AAGGCCTGTT TTTTCCAAGT CTTGGATGCT 
GAGCAAAAAA ACAACTTTGT TGTTGAAAAA TGTAAAACAA AAAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870819.1
CDS	23..913
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKFFLCVKRSFDVLVFALRTDVSVLKQLVICKLDIWSKSNYQVFQKACFFQVLDAEQKNNFVVEKCKTKKY

Target ORF information:

RefSeq Version	XM_017015330.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015330.2

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GTTCTTCCTG TGTGTCAAGA GGTCTTTTGA TGTCTTGGTG 
TTTGCCCTGA GAACAGACGT GTCTGTGCTG AAGCAATTGG TCATCTGCAA GCTTGATATA 
TGGTCCAAAT CCAACTATCA AGTATTCCAG AAGGCCTGTT TTTTCCAAGT CTTGGATGCT 
GAGCAAAAAA ACAACTTTGT TGTTGAAAAA TGTAAAACAA AAAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED27 ( XM_017015330.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870819.1 Homo sapiens mediator complex subunit 27 (MED27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99021
Clone ID Related Accession (Same CDS sequence)	XM_017015330.2 , XM_017015330.1
Accession Version	XM_017015330.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 891bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GTTCTTCCTG TGTGTCAAGA GGTCTTTTGA TGTCTTGGTG 
TTTGCCCTGA GAACAGACGT GTCTGTGCTG AAGCAATTGG TCATCTGCAA GCTTGATATA 
TGGTCCAAAT CCAACTATCA AGTATTCCAG AAGGCCTGTT TTTTCCAAGT CTTGGATGCT 
GAGCAAAAAA ACAACTTTGT TGTTGAAAAA TGTAAAACAA AAAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870819.1
CDS	23..913
Translation	MADVINVSVNLEAFSQAISAIQALRSSVSRVFDCLKDGMRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLLQAYKWSNKLQYHAGLASGLLNQQSLKRSANQMGVSAKRRPKAQPTTLVLPPQYVDDVISRIDRMFPEMSIHLSRPNGTSAMLLVTLGKVLKVIVVMRSLFIDRTIVKGYNENVYTEDGKFFLCVKRSFDVLVFALRTDVSVLKQLVICKLDIWSKSNYQVFQKACFFQVLDAEQKNNFVVEKCKTKKY

Target ORF information:

RefSeq Version	XM_017015330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 27 (MED27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015330.1

ORF Insert Sequence:

ATGGCGGACG TGATAAATGT CAGTGTGAAC CTGGAGGCCT TTTCCCAGGC CATTAGTGCC 
ATCCAGGCGC TGCGCTCCAG CGTGAGCAGG GTGTTCGACT GCCTGAAGGA TGGGATGCGG 
AACAAGGAGA CGCTGGAGGG CCGGGAGAAG GCCTTTATTG CGCACTTCCA GGACAACTTA 
CATTCGGTCA ACCGGGACCT CAATGAGCTG GAACGTCTGA GCAATCTGGT AGGCAAGCCA 
TCTGAGAACC ATCCTCTTCA TAACAGTGGG CTGTTAAGCC TGGATCCTGT GCAGGACAAA 
ACTCCTCTCT ATAGTCAACT CCTTCAAGCA TATAAGTGGT CAAACAAGTT GCAGTACCAT 
GCAGGACTAG CATCTGGCCT TTTAAATCAG CAGTCATTGA AGCGTTCCGC TAATCAGATG 
GGAGTATCTG CCAAACGTAG ACCAAAGGCT CAGCCCACAA CTCTTGTCCT ACCACCTCAA 
TATGTTGATG ATGTGATCAG CCGCATTGAC AGGATGTTTC CTGAAATGTC CATCCACTTA 
TCCAGACCCA ATGGAACATC AGCAATGCTT CTGGTGACCT TGGGAAAGGT GTTGAAAGTG 
ATCGTCGTCA TGCGGAGCCT GTTCATTGAT CGAACAATAG TAAAGGGATA TAACGAGAAT 
GTCTACACAG AAGATGGCAA GTTCTTCCTG TGTGTCAAGA GGTCTTTTGA TGTCTTGGTG 
TTTGCCCTGA GAACAGACGT GTCTGTGCTG AAGCAATTGG TCATCTGCAA GCTTGATATA 
TGGTCCAAAT CCAACTATCA AGTATTCCAG AAGGCCTGTT TTTTCCAAGT CTTGGATGCT 
GAGCAAAAAA ACAACTTTGT TGTTGAAAAA TGTAAAACAA AAAAATATTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MED27
Entrez Gene ID	9442
Full Name	mediator complex subunit 27
Synonyms	CRAP34,CRSP34,CRSP8,MED3,TRAP37
General protein information	Preferred Names mediator complex subunit 27 Names mediator of RNA polymerase II transcription subunit 27 CRSP complex subunit 8 cofactor required for Sp1 transcriptional activation, subunit 8, 34kDa p37 TRAP/SMCC/PC2 subunit transcriptional coactivator CRSP34
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.13
Summary	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011].
Disorder MIM:	605044

	MED27	XP_001105126.1
Danio rerio (zebrafish)	med27	NP_956954.1
Mus musculus (house mouse)	Med27	NP_081172.2
Anopheles gambiae (African malaria mosquito)	MED27_ANOGA	XP_312260.3
Homo sapiens (human)	MED27	NP_004260.2
Rattus norvegicus (Norway rat)	Med27	NP_001100035.1
Gallus gallus (chicken)	MED27	NP_001264204.1
Drosophila melanogaster (fruit fly)	MED27	NP_649569.1
Bos taurus (cattle)	MED27	NP_001035636.1
Canis lupus familiaris (dog)	MED27	XP_849826.1
Xenopus tropicalis (tropical clawed frog)	med27	XP_004916727.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MED27 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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