MED26 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED26
Entrez Gene ID	9441
Full Name	mediator complex subunit 26
Synonyms	CRSP7,CRSP70
General protein information	Preferred Names mediator complex subunit 26 Names mediator of RNA polymerase II transcription subunit 26 ARC70 CRSP complex subunit 7 activator-recruited cofactor 70 kDa component cofactor required for Sp1 transcriptional activation subunit 7 cofactor required for Sp1 transcriptional activation, subunit 7 (70kD) cofactor required for Sp1 transcriptional activation, subunit 7, 70kDa transcriptional coactivator CRSP70
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Summary	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008].
Disorder MIM:	605043

mRNA and Protein(s)

mRNA	Protein	Name
NM_004831.3	NP_004822.2	mediator of RNA polymerase II transcription subunit 26
NM_004831.4	NP_004822.2	mediator of RNA polymerase II transcription subunit 26
NM_004831.5	NP_004822.2	mediator of RNA polymerase II transcription subunit 26

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MED26	NP_001092358.1
Gallus gallus (chicken)	MED26	XP_004948991.1
Danio rerio (zebrafish)	crsp7	NP_998224.1
Homo sapiens (human)	MED26	NP_004822.2
Pan troglodytes (chimpanzee)	MED26	XP_003953452.1
Xenopus tropicalis (tropical clawed frog)	med26	NP_001016996.1
Canis lupus familiaris (dog)	MED26	XP_541963.3
Mus musculus (house mouse)	Med26	NP_081761.2
Rattus norvegicus (Norway rat)	Med26	NP_001100770.2

Gene Ontology
Bibliography

Related articles in PubMed

Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.
Lens Z, Cantrelle FX, Peruzzini R, Hanoulle X, Dewitte F, Ferreira E, Baert JL, Mont? D, Aumercier M, Villeret V, Verger A, Landrieu I
Journal of molecular biology429(20)3043-3055(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.
Takahashi H, Takigawa I, Watanabe M, Anwar D, Shibata M, Tomomori-Sato C, Sato S, Ranjan A, Seidel CW, Tsukiyama T, Mizushima W, Hayashi M, Ohkawa Y, Conaway JW, Conaway RC, Hatakeyama S
Nature communications65941(2015 Jan)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

Human mediator subunit MED26 functions as a docking site for transcription elongation factors.
Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE, Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
Cell146(1)92-104(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

EAF1 (239-268) and TAF7 (205-235) peptide interactions were both mapped to the same groove formed by H3 and H4 helices of MED26 N-terminal domain.
Title: Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.

the MED26-NTD functions as a molecular switch in the exchange of TBP-associated factor 7 (TAF7) for LEC to facilitate the transition from initiation to elongation during transcription of a subset of snRNA genes
Title: MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.

MED26 can function as a molecular switch that interacts first with TFIID in the Pol II initiation complex and then exchanges TFIID for complexes containing ELL/EAF and P-TEFb to facilitate transition of Pol II into the elongation stage of transcription.
Title: Human mediator subunit MED26 functions as a docking site for transcription elongation factors.

findings identify MED19/MED26 as a probable composite REST interface in Mediator and further clarify the mechanistic basis by which Mediator facilitates REST-imposed epigenetic restrictions on neuronal gene expression
Title: MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression.

Human CRSP interacts with RNA polymerase II CTD and adopts a specific CTD-bound conformation
Title: Human CRSP interacts with RNA polymerase II CTD and adopts a specific CTD-bound conformation.

The following MED26 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED26 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04646	NM_004831.5 Latest version!	Homo sapiens mediator complex subunit 26 (MED26), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu04646	NM_004831.3	Homo sapiens mediator complex subunit 26 (MED26), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu04646	NM_004831.4	Homo sapiens mediator complex subunit 26 (MED26), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED26 ( NM_004831.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004822.2 Homo sapiens mediator complex subunit 26 (MED26), mRNA.

Price & Availability↑

CloneID	OHu04646
Clone ID Related Accession (Same CDS sequence)	NM_004831.3 , NM_004831.4 , NM_004831.5
Accession Version	NM_004831.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 26
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC024075.4, AF104253.1 and AK128435.1. On Aug 16, 2005 this sequence version replaced NM_004831.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB121973.1, BC110430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGACAGCGG CTCCGGCGTC TCCGCAGCAG ATCAGGGACC GGCTGCTGCA GGCCATCGAC 
CCCCAGAGCA ACATCCGGAA CATGGTGGCG GTGCTGGAAG TCATCTCCAG CCTGGAGAAA 
TACCCTATTA CCAAAGAGGC ACTTGAGGAA ACACGACTTG GGAAGCTCAT CAACGACGTC 
CGCAAGAAAA CCAAGAACGA GGAGCTCGCC AAGCGGGCCA AGAAGCTGCT GCGGAGCTGG 
CAGAAGCTCA TCGAGCCGGC ACACCAGCAT GAGGCGGCGC TGCGGGGGCT GGCGGGGGCC 
ACCGGCTCTG CCAACGGGGG CGCACACAAC TGCCGGCCGG AGGTGGGGGC GGCTGGCCCA 
CCCAGGAGCA TCCATGACCT GAAGAGCCGC AATGACCTCC AGAGGCTGCC CGGGCAGCGG 
CTGGACAGGC TGGGCAGCCG CAAGCGCCGG GGTGACCAGC GTGACCTCGG CCACCCAGGG 
CCGCCACCCA AGGTCTCCAA AGCTAGCCAC GACCCCCTGG TCCCCAACTC ATCCCCCCTC 
CCCACCAACG GGATCAGTGG GAGTCCAGAG AGCTTCGCCA GCTCCCTGGA TGGCAGTGGG 
CATGCAGGCC CAGAGGGCAG CCGCCTGGAG CGTGACGAGA ATGACAAGCA CAGTGGCAAG 
ATCCCCGTCA ACGCCGTGCG ACCGCACACC AGCTCCCCGG GCCTGGGCAA GCCCCCTGGA 
CCCTGCTTGC AGCCAAAGGC TTCGGTGCTG CAGCAGCTGG ACAGGGTGGA CGAGACTCCG 
GGGCCTCCCC ATCCCAAGGG ACCCCCTCGC TGCTCTTTCA GTCCTCGGAA CTCACGGCAT 
GAGGGCTCCT TTGCCCGGCA GCAGAGCTTG TATGCACCCA AGGGCTCCGT GCCCAGCCCC 
TCACCGCGGC CCCAGGCACT CGATGCCACA CAGGTGCCGT CACCGCTTCC ACTGGCACAG 
CCGTCCACAC CCCCCGTACG GCGGCTCGAG CTGCTGCCCA GTGCGGAAAG CCCAGTGTGC 
TGGCTTGAGC AGCCTGAGAG CCACCAGCGG CTGGCGGGGC CGGGCTGCAA GGCAGGGCTG 
TCCCCAGCCG AGCCCCTCCT GTCCCGGGCA GGCTTTTCCC CAGACTCCTC CAAGGCGGAC 
AGTGATGCTG CCTCCTCAGG GGGCTCGGAC AGTAAAAAGA AGAAGAGGTA CCGACCTCGA 
GACTATACGG TTAACTTGGA CGGGCAGGTG GCTGAGGCGG GCGTCAAGCC TGTCCGGTTA 
AAAGAGCGGA AGCTCACCTT TGACCCCATG ACGAGACAGA TCAAACCTCT GACCCAGAAA 
GAGCCAGTGC GGGCAGACAG CCCTGTGCAC ATGGAGCAGC AGTCCAGGAC AGAGCTGGAC 
AAGCAGGAGG CCAAGGCCAG CCTCCAGAGC CCCTTCGAAC AGACGAACTG GAAGGAGCTG 
TCACGCAACG AGATCATCCA GTCCTACCTG AGCCGGCAGA GCAGCCTGCT CTCATCATCG 
GGCGCGCAGA CCCCAGGGGC TCACCACTTC ATGTCTGAGT ACCTGAAGCA GGAGGAGAGC 
ACCCGGCAAG GGGCCAGGCA GCTGCATGTG CTGGTGCCTC AAAGCCCGCC CACGGACCTC 
CCTGGTCTGA CCCGGGAGGT CACACAGGAC GATCTCGACA GAATCCAGGC CAGCCAGTGG 
CCGGGGGTGA ACGGGTGTCA GGACACACAG GGTAACTGGT ATGACTGGAC GCAGTGCATA 
TCGCTCGATC CGCACGGCGA CGACGGGCGC TTGAACATTC TGCCTTATGT CTGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004822.2
CDS	262..2064
Translation	MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPITKEALEETRLGKLINDVRKKTKNEELAKRAKKLLRSWQKLIEPAHQHEAALRGLAGATGSANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQRLDRLGSRKRRGDQRDLGHPGPPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLERDENDKHSGKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPRNSRHEGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPSAESPVCWLEQPESHQRLAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSKKKKRYRPRDYTVNLDGQVAEAGVKPVRLKERKLTFDPMTRQIKPLTQKEPVRADSPVHMEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYLSRQSSLLSSSGAQTPGAHHFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQWPGVNGCQDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD

Target ORF information:

RefSeq Version	NM_004831.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 26 (MED26), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004831.3

ORF Insert Sequence:

ATGACAGCGG CTCCGGCGTC TCCGCAGCAG ATCAGGGACC GGCTGCTGCA GGCCATCGAC 
CCCCAGAGCA ACATCCGGAA CATGGTGGCG GTGCTGGAAG TCATCTCCAG CCTGGAGAAA 
TACCCTATTA CCAAAGAGGC ACTTGAGGAA ACACGACTTG GGAAGCTCAT CAACGACGTC 
CGCAAGAAAA CCAAGAACGA GGAGCTCGCC AAGCGGGCCA AGAAGCTGCT GCGGAGCTGG 
CAGAAGCTCA TCGAGCCGGC ACACCAGCAT GAGGCGGCGC TGCGGGGGCT GGCGGGGGCC 
ACCGGCTCTG CCAACGGGGG CGCACACAAC TGCCGGCCGG AGGTGGGGGC GGCTGGCCCA 
CCCAGGAGCA TCCATGACCT GAAGAGCCGC AATGACCTCC AGAGGCTGCC CGGGCAGCGG 
CTGGACAGGC TGGGCAGCCG CAAGCGCCGG GGTGACCAGC GTGACCTCGG CCACCCAGGG 
CCGCCACCCA AGGTCTCCAA AGCTAGCCAC GACCCCCTGG TCCCCAACTC ATCCCCCCTC 
CCCACCAACG GGATCAGTGG GAGTCCAGAG AGCTTCGCCA GCTCCCTGGA TGGCAGTGGG 
CATGCAGGCC CAGAGGGCAG CCGCCTGGAG CGTGACGAGA ATGACAAGCA CAGTGGCAAG 
ATCCCCGTCA ACGCCGTGCG ACCGCACACC AGCTCCCCGG GCCTGGGCAA GCCCCCTGGA 
CCCTGCTTGC AGCCAAAGGC TTCGGTGCTG CAGCAGCTGG ACAGGGTGGA CGAGACTCCG 
GGGCCTCCCC ATCCCAAGGG ACCCCCTCGC TGCTCTTTCA GTCCTCGGAA CTCACGGCAT 
GAGGGCTCCT TTGCCCGGCA GCAGAGCTTG TATGCACCCA AGGGCTCCGT GCCCAGCCCC 
TCACCGCGGC CCCAGGCACT CGATGCCACA CAGGTGCCGT CACCGCTTCC ACTGGCACAG 
CCGTCCACAC CCCCCGTACG GCGGCTCGAG CTGCTGCCCA GTGCGGAAAG CCCAGTGTGC 
TGGCTTGAGC AGCCTGAGAG CCACCAGCGG CTGGCGGGGC CGGGCTGCAA GGCAGGGCTG 
TCCCCAGCCG AGCCCCTCCT GTCCCGGGCA GGCTTTTCCC CAGACTCCTC CAAGGCGGAC 
AGTGATGCTG CCTCCTCAGG GGGCTCGGAC AGTAAAAAGA AGAAGAGGTA CCGACCTCGA 
GACTATACGG TTAACTTGGA CGGGCAGGTG GCTGAGGCGG GCGTCAAGCC TGTCCGGTTA 
AAAGAGCGGA AGCTCACCTT TGACCCCATG ACGAGACAGA TCAAACCTCT GACCCAGAAA 
GAGCCAGTGC GGGCAGACAG CCCTGTGCAC ATGGAGCAGC AGTCCAGGAC AGAGCTGGAC 
AAGCAGGAGG CCAAGGCCAG CCTCCAGAGC CCCTTCGAAC AGACGAACTG GAAGGAGCTG 
TCACGCAACG AGATCATCCA GTCCTACCTG AGCCGGCAGA GCAGCCTGCT CTCATCATCG 
GGCGCGCAGA CCCCAGGGGC TCACCACTTC ATGTCTGAGT ACCTGAAGCA GGAGGAGAGC 
ACCCGGCAAG GGGCCAGGCA GCTGCATGTG CTGGTGCCTC AAAGCCCGCC CACGGACCTC 
CCTGGTCTGA CCCGGGAGGT CACACAGGAC GATCTCGACA GAATCCAGGC CAGCCAGTGG 
CCGGGGGTGA ACGGGTGTCA GGACACACAG GGTAACTGGT ATGACTGGAC GCAGTGCATA 
TCGCTCGATC CGCACGGCGA CGACGGGCGC TTGAACATTC TGCCTTATGT CTGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED26 ( NM_004831.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004822.2 Homo sapiens mediator complex subunit 26 (MED26), mRNA.

Price & Availability↑

CloneID	OHu04646
Clone ID Related Accession (Same CDS sequence)	NM_004831.3 , NM_004831.4 , NM_004831.5
Accession Version	NM_004831.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 26
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC024075.4, AF104253.1 and AK128435.1. On Jun 2, 2019 this sequence version replaced NM_004831.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110430.1, AK313778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGACAGCGG CTCCGGCGTC TCCGCAGCAG ATCAGGGACC GGCTGCTGCA GGCCATCGAC 
CCCCAGAGCA ACATCCGGAA CATGGTGGCG GTGCTGGAAG TCATCTCCAG CCTGGAGAAA 
TACCCTATTA CCAAAGAGGC ACTTGAGGAA ACACGACTTG GGAAGCTCAT CAACGACGTC 
CGCAAGAAAA CCAAGAACGA GGAGCTCGCC AAGCGGGCCA AGAAGCTGCT GCGGAGCTGG 
CAGAAGCTCA TCGAGCCGGC ACACCAGCAT GAGGCGGCGC TGCGGGGGCT GGCGGGGGCC 
ACCGGCTCTG CCAACGGGGG CGCACACAAC TGCCGGCCGG AGGTGGGGGC GGCTGGCCCA 
CCCAGGAGCA TCCATGACCT GAAGAGCCGC AATGACCTCC AGAGGCTGCC CGGGCAGCGG 
CTGGACAGGC TGGGCAGCCG CAAGCGCCGG GGTGACCAGC GTGACCTCGG CCACCCAGGG 
CCGCCACCCA AGGTCTCCAA AGCTAGCCAC GACCCCCTGG TCCCCAACTC ATCCCCCCTC 
CCCACCAACG GGATCAGTGG GAGTCCAGAG AGCTTCGCCA GCTCCCTGGA TGGCAGTGGG 
CATGCAGGCC CAGAGGGCAG CCGCCTGGAG CGTGACGAGA ATGACAAGCA CAGTGGCAAG 
ATCCCCGTCA ACGCCGTGCG ACCGCACACC AGCTCCCCGG GCCTGGGCAA GCCCCCTGGA 
CCCTGCTTGC AGCCAAAGGC TTCGGTGCTG CAGCAGCTGG ACAGGGTGGA CGAGACTCCG 
GGGCCTCCCC ATCCCAAGGG ACCCCCTCGC TGCTCTTTCA GTCCTCGGAA CTCACGGCAT 
GAGGGCTCCT TTGCCCGGCA GCAGAGCTTG TATGCACCCA AGGGCTCCGT GCCCAGCCCC 
TCACCGCGGC CCCAGGCACT CGATGCCACA CAGGTGCCGT CACCGCTTCC ACTGGCACAG 
CCGTCCACAC CCCCCGTACG GCGGCTCGAG CTGCTGCCCA GTGCGGAAAG CCCAGTGTGC 
TGGCTTGAGC AGCCTGAGAG CCACCAGCGG CTGGCGGGGC CGGGCTGCAA GGCAGGGCTG 
TCCCCAGCCG AGCCCCTCCT GTCCCGGGCA GGCTTTTCCC CAGACTCCTC CAAGGCGGAC 
AGTGATGCTG CCTCCTCAGG GGGCTCGGAC AGTAAAAAGA AGAAGAGGTA CCGACCTCGA 
GACTATACGG TTAACTTGGA CGGGCAGGTG GCTGAGGCGG GCGTCAAGCC TGTCCGGTTA 
AAAGAGCGGA AGCTCACCTT TGACCCCATG ACGAGACAGA TCAAACCTCT GACCCAGAAA 
GAGCCAGTGC GGGCAGACAG CCCTGTGCAC ATGGAGCAGC AGTCCAGGAC AGAGCTGGAC 
AAGCAGGAGG CCAAGGCCAG CCTCCAGAGC CCCTTCGAAC AGACGAACTG GAAGGAGCTG 
TCACGCAACG AGATCATCCA GTCCTACCTG AGCCGGCAGA GCAGCCTGCT CTCATCATCG 
GGCGCGCAGA CCCCAGGGGC TCACCACTTC ATGTCTGAGT ACCTGAAGCA GGAGGAGAGC 
ACCCGGCAAG GGGCCAGGCA GCTGCATGTG CTGGTGCCTC AAAGCCCGCC CACGGACCTC 
CCTGGTCTGA CCCGGGAGGT CACACAGGAC GATCTCGACA GAATCCAGGC CAGCCAGTGG 
CCGGGGGTGA ACGGGTGTCA GGACACACAG GGTAACTGGT ATGACTGGAC GCAGTGCATA 
TCGCTCGATC CGCACGGCGA CGACGGGCGC TTGAACATTC TGCCTTATGT CTGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004822.2
CDS	262..2064
Translation	MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPITKEALEETRLGKLINDVRKKTKNEELAKRAKKLLRSWQKLIEPAHQHEAALRGLAGATGSANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQRLDRLGSRKRRGDQRDLGHPGPPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLERDENDKHSGKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPRNSRHEGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPSAESPVCWLEQPESHQRLAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSKKKKRYRPRDYTVNLDGQVAEAGVKPVRLKERKLTFDPMTRQIKPLTQKEPVRADSPVHMEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYLSRQSSLLSSSGAQTPGAHHFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQWPGVNGCQDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD

Target ORF information:

RefSeq Version	NM_004831.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 26 (MED26), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004831.4

ORF Insert Sequence:

ATGACAGCGG CTCCGGCGTC TCCGCAGCAG ATCAGGGACC GGCTGCTGCA GGCCATCGAC 
CCCCAGAGCA ACATCCGGAA CATGGTGGCG GTGCTGGAAG TCATCTCCAG CCTGGAGAAA 
TACCCTATTA CCAAAGAGGC ACTTGAGGAA ACACGACTTG GGAAGCTCAT CAACGACGTC 
CGCAAGAAAA CCAAGAACGA GGAGCTCGCC AAGCGGGCCA AGAAGCTGCT GCGGAGCTGG 
CAGAAGCTCA TCGAGCCGGC ACACCAGCAT GAGGCGGCGC TGCGGGGGCT GGCGGGGGCC 
ACCGGCTCTG CCAACGGGGG CGCACACAAC TGCCGGCCGG AGGTGGGGGC GGCTGGCCCA 
CCCAGGAGCA TCCATGACCT GAAGAGCCGC AATGACCTCC AGAGGCTGCC CGGGCAGCGG 
CTGGACAGGC TGGGCAGCCG CAAGCGCCGG GGTGACCAGC GTGACCTCGG CCACCCAGGG 
CCGCCACCCA AGGTCTCCAA AGCTAGCCAC GACCCCCTGG TCCCCAACTC ATCCCCCCTC 
CCCACCAACG GGATCAGTGG GAGTCCAGAG AGCTTCGCCA GCTCCCTGGA TGGCAGTGGG 
CATGCAGGCC CAGAGGGCAG CCGCCTGGAG CGTGACGAGA ATGACAAGCA CAGTGGCAAG 
ATCCCCGTCA ACGCCGTGCG ACCGCACACC AGCTCCCCGG GCCTGGGCAA GCCCCCTGGA 
CCCTGCTTGC AGCCAAAGGC TTCGGTGCTG CAGCAGCTGG ACAGGGTGGA CGAGACTCCG 
GGGCCTCCCC ATCCCAAGGG ACCCCCTCGC TGCTCTTTCA GTCCTCGGAA CTCACGGCAT 
GAGGGCTCCT TTGCCCGGCA GCAGAGCTTG TATGCACCCA AGGGCTCCGT GCCCAGCCCC 
TCACCGCGGC CCCAGGCACT CGATGCCACA CAGGTGCCGT CACCGCTTCC ACTGGCACAG 
CCGTCCACAC CCCCCGTACG GCGGCTCGAG CTGCTGCCCA GTGCGGAAAG CCCAGTGTGC 
TGGCTTGAGC AGCCTGAGAG CCACCAGCGG CTGGCGGGGC CGGGCTGCAA GGCAGGGCTG 
TCCCCAGCCG AGCCCCTCCT GTCCCGGGCA GGCTTTTCCC CAGACTCCTC CAAGGCGGAC 
AGTGATGCTG CCTCCTCAGG GGGCTCGGAC AGTAAAAAGA AGAAGAGGTA CCGACCTCGA 
GACTATACGG TTAACTTGGA CGGGCAGGTG GCTGAGGCGG GCGTCAAGCC TGTCCGGTTA 
AAAGAGCGGA AGCTCACCTT TGACCCCATG ACGAGACAGA TCAAACCTCT GACCCAGAAA 
GAGCCAGTGC GGGCAGACAG CCCTGTGCAC ATGGAGCAGC AGTCCAGGAC AGAGCTGGAC 
AAGCAGGAGG CCAAGGCCAG CCTCCAGAGC CCCTTCGAAC AGACGAACTG GAAGGAGCTG 
TCACGCAACG AGATCATCCA GTCCTACCTG AGCCGGCAGA GCAGCCTGCT CTCATCATCG 
GGCGCGCAGA CCCCAGGGGC TCACCACTTC ATGTCTGAGT ACCTGAAGCA GGAGGAGAGC 
ACCCGGCAAG GGGCCAGGCA GCTGCATGTG CTGGTGCCTC AAAGCCCGCC CACGGACCTC 
CCTGGTCTGA CCCGGGAGGT CACACAGGAC GATCTCGACA GAATCCAGGC CAGCCAGTGG 
CCGGGGGTGA ACGGGTGTCA GGACACACAG GGTAACTGGT ATGACTGGAC GCAGTGCATA 
TCGCTCGATC CGCACGGCGA CGACGGGCGC TTGAACATTC TGCCTTATGT CTGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED26 ( NM_004831.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004822.2 Homo sapiens mediator complex subunit 26 (MED26), mRNA.

Price & Availability↑

CloneID	OHu04646
Clone ID Related Accession (Same CDS sequence)	NM_004831.3 , NM_004831.4 , NM_004831.5
Accession Version	NM_004831.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1803bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 26
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC024075.4, AF104253.1, AK128435.1 and AC008764.9. On Nov 30, 2019 this sequence version replaced NM_004831.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC110430.1, AK313778.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGACAGCGG CTCCGGCGTC TCCGCAGCAG ATCAGGGACC GGCTGCTGCA GGCCATCGAC 
CCCCAGAGCA ACATCCGGAA CATGGTGGCG GTGCTGGAAG TCATCTCCAG CCTGGAGAAA 
TACCCTATTA CCAAAGAGGC ACTTGAGGAA ACACGACTTG GGAAGCTCAT CAACGACGTC 
CGCAAGAAAA CCAAGAACGA GGAGCTCGCC AAGCGGGCCA AGAAGCTGCT GCGGAGCTGG 
CAGAAGCTCA TCGAGCCGGC ACACCAGCAT GAGGCGGCGC TGCGGGGGCT GGCGGGGGCC 
ACCGGCTCTG CCAACGGGGG CGCACACAAC TGCCGGCCGG AGGTGGGGGC GGCTGGCCCA 
CCCAGGAGCA TCCATGACCT GAAGAGCCGC AATGACCTCC AGAGGCTGCC CGGGCAGCGG 
CTGGACAGGC TGGGCAGCCG CAAGCGCCGG GGTGACCAGC GTGACCTCGG CCACCCAGGG 
CCGCCACCCA AGGTCTCCAA AGCTAGCCAC GACCCCCTGG TCCCCAACTC ATCCCCCCTC 
CCCACCAACG GGATCAGTGG GAGTCCAGAG AGCTTCGCCA GCTCCCTGGA TGGCAGTGGG 
CATGCAGGCC CAGAGGGCAG CCGCCTGGAG CGTGACGAGA ATGACAAGCA CAGTGGCAAG 
ATCCCCGTCA ACGCCGTGCG ACCGCACACC AGCTCCCCGG GCCTGGGCAA GCCCCCTGGA 
CCCTGCTTGC AGCCAAAGGC TTCGGTGCTG CAGCAGCTGG ACAGGGTGGA CGAGACTCCG 
GGGCCTCCCC ATCCCAAGGG ACCCCCTCGC TGCTCTTTCA GTCCTCGGAA CTCACGGCAT 
GAGGGCTCCT TTGCCCGGCA GCAGAGCTTG TATGCACCCA AGGGCTCCGT GCCCAGCCCC 
TCACCGCGGC CCCAGGCACT CGATGCCACA CAGGTGCCGT CACCGCTTCC ACTGGCACAG 
CCGTCCACAC CCCCCGTACG GCGGCTCGAG CTGCTGCCCA GTGCGGAAAG CCCAGTGTGC 
TGGCTTGAGC AGCCTGAGAG CCACCAGCGG CTGGCGGGGC CGGGCTGCAA GGCAGGGCTG 
TCCCCAGCCG AGCCCCTCCT GTCCCGGGCA GGCTTTTCCC CAGACTCCTC CAAGGCGGAC 
AGTGATGCTG CCTCCTCAGG GGGCTCGGAC AGTAAAAAGA AGAAGAGGTA CCGACCTCGA 
GACTATACGG TTAACTTGGA CGGGCAGGTG GCTGAGGCGG GCGTCAAGCC TGTCCGGTTA 
AAAGAGCGGA AGCTCACCTT TGACCCCATG ACGAGACAGA TCAAACCTCT GACCCAGAAA 
GAGCCAGTGC GGGCAGACAG CCCTGTGCAC ATGGAGCAGC AGTCCAGGAC AGAGCTGGAC 
AAGCAGGAGG CCAAGGCCAG CCTCCAGAGC CCCTTCGAAC AGACGAACTG GAAGGAGCTG 
TCACGCAACG AGATCATCCA GTCCTACCTG AGCCGGCAGA GCAGCCTGCT CTCATCATCG 
GGCGCGCAGA CCCCAGGGGC TCACCACTTC ATGTCTGAGT ACCTGAAGCA GGAGGAGAGC 
ACCCGGCAAG GGGCCAGGCA GCTGCATGTG CTGGTGCCTC AAAGCCCGCC CACGGACCTC 
CCTGGTCTGA CCCGGGAGGT CACACAGGAC GATCTCGACA GAATCCAGGC CAGCCAGTGG 
CCGGGGGTGA ACGGGTGTCA GGACACACAG GGTAACTGGT ATGACTGGAC GCAGTGCATA 
TCGCTCGATC CGCACGGCGA CGACGGGCGC TTGAACATTC TGCCTTATGT CTGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004822.2
CDS	262..2064
Translation	MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPITKEALEETRLGKLINDVRKKTKNEELAKRAKKLLRSWQKLIEPAHQHEAALRGLAGATGSANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQRLDRLGSRKRRGDQRDLGHPGPPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLERDENDKHSGKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPRNSRHEGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPSAESPVCWLEQPESHQRLAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSKKKKRYRPRDYTVNLDGQVAEAGVKPVRLKERKLTFDPMTRQIKPLTQKEPVRADSPVHMEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYLSRQSSLLSSSGAQTPGAHHFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQWPGVNGCQDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD

Target ORF information:

RefSeq Version	NM_004831.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 26 (MED26), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004831.5

ORF Insert Sequence:

ATGACAGCGG CTCCGGCGTC TCCGCAGCAG ATCAGGGACC GGCTGCTGCA GGCCATCGAC 
CCCCAGAGCA ACATCCGGAA CATGGTGGCG GTGCTGGAAG TCATCTCCAG CCTGGAGAAA 
TACCCTATTA CCAAAGAGGC ACTTGAGGAA ACACGACTTG GGAAGCTCAT CAACGACGTC 
CGCAAGAAAA CCAAGAACGA GGAGCTCGCC AAGCGGGCCA AGAAGCTGCT GCGGAGCTGG 
CAGAAGCTCA TCGAGCCGGC ACACCAGCAT GAGGCGGCGC TGCGGGGGCT GGCGGGGGCC 
ACCGGCTCTG CCAACGGGGG CGCACACAAC TGCCGGCCGG AGGTGGGGGC GGCTGGCCCA 
CCCAGGAGCA TCCATGACCT GAAGAGCCGC AATGACCTCC AGAGGCTGCC CGGGCAGCGG 
CTGGACAGGC TGGGCAGCCG CAAGCGCCGG GGTGACCAGC GTGACCTCGG CCACCCAGGG 
CCGCCACCCA AGGTCTCCAA AGCTAGCCAC GACCCCCTGG TCCCCAACTC ATCCCCCCTC 
CCCACCAACG GGATCAGTGG GAGTCCAGAG AGCTTCGCCA GCTCCCTGGA TGGCAGTGGG 
CATGCAGGCC CAGAGGGCAG CCGCCTGGAG CGTGACGAGA ATGACAAGCA CAGTGGCAAG 
ATCCCCGTCA ACGCCGTGCG ACCGCACACC AGCTCCCCGG GCCTGGGCAA GCCCCCTGGA 
CCCTGCTTGC AGCCAAAGGC TTCGGTGCTG CAGCAGCTGG ACAGGGTGGA CGAGACTCCG 
GGGCCTCCCC ATCCCAAGGG ACCCCCTCGC TGCTCTTTCA GTCCTCGGAA CTCACGGCAT 
GAGGGCTCCT TTGCCCGGCA GCAGAGCTTG TATGCACCCA AGGGCTCCGT GCCCAGCCCC 
TCACCGCGGC CCCAGGCACT CGATGCCACA CAGGTGCCGT CACCGCTTCC ACTGGCACAG 
CCGTCCACAC CCCCCGTACG GCGGCTCGAG CTGCTGCCCA GTGCGGAAAG CCCAGTGTGC 
TGGCTTGAGC AGCCTGAGAG CCACCAGCGG CTGGCGGGGC CGGGCTGCAA GGCAGGGCTG 
TCCCCAGCCG AGCCCCTCCT GTCCCGGGCA GGCTTTTCCC CAGACTCCTC CAAGGCGGAC 
AGTGATGCTG CCTCCTCAGG GGGCTCGGAC AGTAAAAAGA AGAAGAGGTA CCGACCTCGA 
GACTATACGG TTAACTTGGA CGGGCAGGTG GCTGAGGCGG GCGTCAAGCC TGTCCGGTTA 
AAAGAGCGGA AGCTCACCTT TGACCCCATG ACGAGACAGA TCAAACCTCT GACCCAGAAA 
GAGCCAGTGC GGGCAGACAG CCCTGTGCAC ATGGAGCAGC AGTCCAGGAC AGAGCTGGAC 
AAGCAGGAGG CCAAGGCCAG CCTCCAGAGC CCCTTCGAAC AGACGAACTG GAAGGAGCTG 
TCACGCAACG AGATCATCCA GTCCTACCTG AGCCGGCAGA GCAGCCTGCT CTCATCATCG 
GGCGCGCAGA CCCCAGGGGC TCACCACTTC ATGTCTGAGT ACCTGAAGCA GGAGGAGAGC 
ACCCGGCAAG GGGCCAGGCA GCTGCATGTG CTGGTGCCTC AAAGCCCGCC CACGGACCTC 
CCTGGTCTGA CCCGGGAGGT CACACAGGAC GATCTCGACA GAATCCAGGC CAGCCAGTGG 
CCGGGGGTGA ACGGGTGTCA GGACACACAG GGTAACTGGT ATGACTGGAC GCAGTGCATA 
TCGCTCGATC CGCACGGCGA CGACGGGCGC TTGAACATTC TGCCTTATGT CTGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Solution Structure of the N-Terminal Domain of Mediator Subunit MED26 and Molecular Characterization of Its Interaction with EAF1 and TAF7.
Journal of molecular biology429(20)3043-3055(2017 Oct)
Lens Z,Cantrelle FX,Peruzzini R,Hanoulle X,Dewitte F,Ferreira E,Baert JL,Mont? D,Aumercier M,Villeret V,Verger A,Landrieu I

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

MED26 regulates the transcription of snRNA genes through the recruitment of little elongation complex.
Nature communications65941(2015 Jan)
Takahashi H,Takigawa I,Watanabe M,Anwar D,Shibata M,Tomomori-Sato C,Sato S,Ranjan A,Seidel CW,Tsukiyama T,Mizushima W,Hayashi M,Ohkawa Y,Conaway JW,Conaway RC,Hatakeyama S

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

Human mediator subunit MED26 functions as a docking site for transcription elongation factors.
Cell146(1)92-104(2011 Jul)
Takahashi H,Parmely TJ,Sato S,Tomomori-Sato C,Banks CA,Kong SE,Szutorisz H,Swanson SK,Martin-Brown S,Washburn MP,Florens L,Seidel CW,Lin C,Smith ER,Shilatifard A,Conaway RC,Conaway JW

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MED26 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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