MED17 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED17
Entrez Gene ID	9440
Full Name	mediator complex subunit 17
Synonyms	CRSP6,CRSP77,DRIP80,SRB4,TRAP80
General protein information	Preferred Names mediator complex subunit 17 Names mediator of RNA polymerase II transcription subunit 17 ARC77 CRSP complex subunit 6 activator-recruited cofactor 77 kDa component cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa thyroid hormone receptor-associated protein complex 80 kDa component transcriptional coactivator CRSP77 vitamin D3 receptor-interacting protein complex 80 kDa component
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q21
Summary	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008].
Disorder MIM:	603810
Disorder Html:	Microcephaly, postnatal progressive, with seizures and brain atrophy, 613668 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004268.4	NP_004259.3	mediator of RNA polymerase II transcription subunit 17
XM_011543068.2	XP_011541370.1	mediator of RNA polymerase II transcription subunit 17 isoform X1
NM_004268.5	NP_004259.3	mediator of RNA polymerase II transcription subunit 17

Pathways from BioSystems

Homologies

	MED17	XP_001088365.1
Mus musculus (house mouse)	Med17	NP_659182.1
Gallus gallus (chicken)	MED17	NP_001006280.1
Anopheles gambiae (African malaria mosquito)	MED17_ANOGA	XP_319901.2
Xenopus tropicalis (tropical clawed frog)	med17	NP_001016974.1
Pan troglodytes (chimpanzee)	MED17	XP_508698.3
Canis lupus familiaris (dog)	MED17	XP_533975.3
Bos taurus (cattle)	MED17	NP_001029902.2
Homo sapiens (human)	MED17	NP_004259.3
Rattus norvegicus (Norway rat)	Med17	NP_001100271.1
Danio rerio (zebrafish)	med17	NP_001071042.1
Drosophila melanogaster (fruit fly)	MED17	NP_650686.1
Caenorhabditis elegans (roundworm)	mdt-17	NP_001256910.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Human mediator MED17 subunit plays essential roles in gene regulation by associating with the transcription and DNA repair machineries.
Kikuchi Y, Umemura H, Nishitani S, Iida S, Fukasawa R, Hayashi H, Hirose Y, Tanaka A, Sugasawa K, Ohkuma Y
Genes to cells : devoted to molecular & cellular mechanisms20(3)191-202(2015 Mar)

Hepatic TRAP80 selectively regulates lipogenic activity of liver X receptor.
Kim GH, Oh GS, Yoon J, Lee GG, Lee KU, Kim SW
The Journal of clinical investigation125(1)183-93(2015 Jan)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

Mediator and post-recruitment regulation of RNA polymerase II.
Knuesel MT, Taatjes DJ
Transcription2(1)28-31(2011 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that hMED17 may play essential roles in switching between transcription and nucleotide excision repair.
Title: Human mediator MED17 subunit plays essential roles in gene regulation by associating with the transcription and DNA repair machineries.

TRAP80 is a selective regulator of hepatic lipogenesis and is required for LXR-dependent SREBP-1c activation.
Title: Hepatic TRAP80 selectively regulates lipogenic activity of liver X receptor.

Studies indicate that the activation domain of p53 (p53AD) binds directly to the MED17 subunit of Mediator, whereas the p53 C-terminal domain (p53CTD) binds the MED1 subunit.
Title: Mediator and post-recruitment regulation of RNA polymerase II.

the p. L371P mutation in MED17 is a founder mutation in the Caucasus Jewish community and that homozygosity for this mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination.
Title: Infantile cerebral and cerebellar atrophy is associated with a mutation in the MED17 subunit of the transcription preinitiation mediator complex.

The following MED17 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED17 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18450	NM_004268.5 Latest version!	Homo sapiens mediator complex subunit 17 (MED17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu62823	XM_011543068.2 Latest version!	Homo sapiens mediator complex subunit 17 (MED17), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu18450	NM_004268.4	Homo sapiens mediator complex subunit 17 (MED17), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED17 ( NM_004268.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004259.3 Homo sapiens mediator complex subunit 17 (MED17), mRNA.

Price & Availability↑

CloneID	OHu18450
Clone ID Related Accession (Same CDS sequence)	NM_004268.4 , NM_004268.5
Accession Version	NM_004268.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA921529.1, DA506467.1, AK001674.1 and AP001894.4. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2007 this sequence version replaced NM_004268.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.196289.1, SRR1803611.57049.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGGGG TGCGCGCAGT GCGGATCAGC ATCGAATCGG CCTGCGAGAA GCAGGTCCAT 
GAGGTGGGCC TGGATGGCAC CGAGACGTAC CTGCCCCCGC TGTCCATGTC GCAGAATCTG 
GCGCGTCTGG CCCAGCGGAT AGACTTCAGC CAGGGTTCGG GCTCCGAGGA GGAGGAGGCG 
GCGGGGACCG AGGGCGACGC GCAGGAGTGG CCGGGCGCCG GGTCCAGCGC AGACCAGGAC 
GACGAGGAAG GAGTGGTAAA ATTTCAGCCT TCCCTTTGGC CTTGGGACTC AGTGAGGAAC 
AATTTGAGAA GTGCCCTGAC AGAGATGTGT GTTCTCTATG ATGTTCTCAG TATTGTTAGG 
GATAAAAAAT TTATGACTCT TGATCCTGTC TCTCAGGATG CACTTCCTCC AAAACAGAAT 
CCTCAGACGT TGCAATTGAT ATCTAAAAAG AAGTCACTTG CTGGAGCAGC ACAAATCTTA 
TTGAAGGGGG CAGAAAGACT GACTAAATCA GTTACCGAAA ACCAAGAAAA CAAGCTACAA 
AGAGACTTCA ATTCTGAGCT TTTGCGATTA CGGCAACACT GGAAACTTCG AAAAGTTGGA 
GATAAAATTC TCGGAGATCT GAGCTACAGA AGTGCAGGAT CTCTCTTTCC TCATCATGGT 
ACATTTGAAG TAATAAAGAA TACAGATCTC GATCTGGATA AAAAGATACC TGAAGATTAC 
TGTCCTCTTG ATGTCCAAAT TCCTAGTGAT TTAGAGGGGT CTGCATATAT CAAGGTTTCA 
ATACAAAAAC AGGCTCCAGA TATAGGTGAC CTCGGCACAG TTAACCTCTT CAAACGACCT 
TTGCCCAAAT CCAAACCAGG TTCCCCACAT TGGCAGACAA AATTAGAAGC GGCACAGAAT 
GTTCTCTTAT GTAAAGAAAT TTTTGCACAG CTCTCTCGGG AAGCTGTTCA AATTAAATCA 
CAAGTCCCTC ACATTGTGGT GAAAAACCAG ATTATCTCTC AGCCCTTTCC GAGCTTGCAG 
TTATCTATTT CTTTGTGCCA TTCCTCAAAT GATAAGAAAT CCCAAAAATT TGCTACTGAG 
AAGCAATGTC CGGAGGACCA CCTTTATGTC CTAGAGCATA ATTTGCATCT ACTGATTAGA 
GAGTTTCATA AACAGACCTT GAGTTCCATC ATGATGCCTC ATCCAGCAAG TGCACCTTTT 
GGCCACAAGA GAATGAGACT TTCGGGTCCT CAAGCTTTTG ATAAAAATGA AATTAATTCA 
TTACAGTCCA GTGAAGGGCT TCTGGAAAAA ATAATTAAAC AAGCAAAGCA TATTTTTCTA 
AGGAGTAGAG CTGCTGCAAC CATTGACAGC TTAGCAAGCC GAATTGAGGA TCCTCAGATA 
CAGGCTCATT GGTCAAATAT CAATGATGTT TATGAATCTA GTGTGAAAGT TTTAATCACA 
TCACAAGGCT ATGAACAAAT ATGCAAGTCC ATTCAACTGC AATTGAATAT TGGAGTTGAG 
CAGATTCGAG TTGTACATAG AGATGGAAGA GTAATTACAC TGTCTTATCA GGAGCAGGAG 
CTACAGGATT TTCTTCTGTC TCAGATGTCA CAGCACCAGG TACATGCAGT TCAGCAACTC 
GCCAAGGTTA TGGGCTGGCA AGTACTGAGC TTCAGTAATC ATGTGGGACT TGGACCTATA 
GAGAGCATTG GTAATGCATC TGCCATCACG GTGGCCTCCC CAAGTGGTGA CTATGCTATT 
TCAGTTCGTA ATGGACCTGA AAGTGGCAGC AAGATTATGG TTCAGTTTCC TCGTAACCAA 
TGTAAAGACC TTCCAAAAAG TGATGTTTTA CAAGATAACA AATGGAGTCA TCTTCGTGGG 
CCATTCAAAG AAGTTCAGTG GAATAAAATG GAAGGTCGAA ATTTTGTTTA TAAAATGGAG 
CTGCTTATGT CTGCACTTAG CCCTTGTCTA CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004259.3
CDS	276..2231
Translation	MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	NM_004268.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 17 (MED17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004268.4

ORF Insert Sequence:

ATGTCCGGGG TGCGCGCAGT GCGGATCAGC ATCGAATCGG CCTGCGAGAA GCAGGTCCAT 
GAGGTGGGCC TGGATGGCAC CGAGACGTAC CTGCCCCCGC TGTCCATGTC GCAGAATCTG 
GCGCGTCTGG CCCAGCGGAT AGACTTCAGC CAGGGTTCGG GCTCCGAGGA GGAGGAGGCG 
GCGGGGACCG AGGGCGACGC GCAGGAGTGG CCGGGCGCCG GGTCCAGCGC AGACCAGGAC 
GACGAGGAAG GAGTGGTAAA ATTTCAGCCT TCCCTTTGGC CTTGGGACTC AGTGAGGAAC 
AATTTGAGAA GTGCCCTGAC AGAGATGTGT GTTCTCTATG ATGTTCTCAG TATTGTTAGG 
GATAAAAAAT TTATGACTCT TGATCCTGTC TCTCAGGATG CACTTCCTCC AAAACAGAAT 
CCTCAGACGT TGCAATTGAT ATCTAAAAAG AAGTCACTTG CTGGAGCAGC ACAAATCTTA 
TTGAAGGGGG CAGAAAGACT GACTAAATCA GTTACCGAAA ACCAAGAAAA CAAGCTACAA 
AGAGACTTCA ATTCTGAGCT TTTGCGATTA CGGCAACACT GGAAACTTCG AAAAGTTGGA 
GATAAAATTC TCGGAGATCT GAGCTACAGA AGTGCAGGAT CTCTCTTTCC TCATCATGGT 
ACATTTGAAG TAATAAAGAA TACAGATCTC GATCTGGATA AAAAGATACC TGAAGATTAC 
TGTCCTCTTG ATGTCCAAAT TCCTAGTGAT TTAGAGGGGT CTGCATATAT CAAGGTTTCA 
ATACAAAAAC AGGCTCCAGA TATAGGTGAC CTCGGCACAG TTAACCTCTT CAAACGACCT 
TTGCCCAAAT CCAAACCAGG TTCCCCACAT TGGCAGACAA AATTAGAAGC GGCACAGAAT 
GTTCTCTTAT GTAAAGAAAT TTTTGCACAG CTCTCTCGGG AAGCTGTTCA AATTAAATCA 
CAAGTCCCTC ACATTGTGGT GAAAAACCAG ATTATCTCTC AGCCCTTTCC GAGCTTGCAG 
TTATCTATTT CTTTGTGCCA TTCCTCAAAT GATAAGAAAT CCCAAAAATT TGCTACTGAG 
AAGCAATGTC CGGAGGACCA CCTTTATGTC CTAGAGCATA ATTTGCATCT ACTGATTAGA 
GAGTTTCATA AACAGACCTT GAGTTCCATC ATGATGCCTC ATCCAGCAAG TGCACCTTTT 
GGCCACAAGA GAATGAGACT TTCGGGTCCT CAAGCTTTTG ATAAAAATGA AATTAATTCA 
TTACAGTCCA GTGAAGGGCT TCTGGAAAAA ATAATTAAAC AAGCAAAGCA TATTTTTCTA 
AGGAGTAGAG CTGCTGCAAC CATTGACAGC TTAGCAAGCC GAATTGAGGA TCCTCAGATA 
CAGGCTCATT GGTCAAATAT CAATGATGTT TATGAATCTA GTGTGAAAGT TTTAATCACA 
TCACAAGGCT ATGAACAAAT ATGCAAGTCC ATTCAACTGC AATTGAATAT TGGAGTTGAG 
CAGATTCGAG TTGTACATAG AGATGGAAGA GTAATTACAC TGTCTTATCA GGAGCAGGAG 
CTACAGGATT TTCTTCTGTC TCAGATGTCA CAGCACCAGG TACATGCAGT TCAGCAACTC 
GCCAAGGTTA TGGGCTGGCA AGTACTGAGC TTCAGTAATC ATGTGGGACT TGGACCTATA 
GAGAGCATTG GTAATGCATC TGCCATCACG GTGGCCTCCC CAAGTGGTGA CTATGCTATT 
TCAGTTCGTA ATGGACCTGA AAGTGGCAGC AAGATTATGG TTCAGTTTCC TCGTAACCAA 
TGTAAAGACC TTCCAAAAAG TGATGTTTTA CAAGATAACA AATGGAGTCA TCTTCGTGGG 
CCATTCAAAG AAGTTCAGTG GAATAAAATG GAAGGTCGAA ATTTTGTTTA TAAAATGGAG 
CTGCTTATGT CTGCACTTAG CCCTTGTCTA CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED17 ( NM_004268.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004259.3 Homo sapiens mediator complex subunit 17 (MED17), mRNA.

Price & Availability↑

CloneID	OHu18450
Clone ID Related Accession (Same CDS sequence)	NM_004268.4 , NM_004268.5
Accession Version	NM_004268.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 17
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA506467.1, AK001674.1 and AP001894.4. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004268.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001674.1, SRR1803617.196289.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000251871.9/ ENSP00000251871.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGGGG TGCGCGCAGT GCGGATCAGC ATCGAATCGG CCTGCGAGAA GCAGGTCCAT 
GAGGTGGGCC TGGATGGCAC CGAGACGTAC CTGCCCCCGC TGTCCATGTC GCAGAATCTG 
GCGCGTCTGG CCCAGCGGAT AGACTTCAGC CAGGGTTCGG GCTCCGAGGA GGAGGAGGCG 
GCGGGGACCG AGGGCGACGC GCAGGAGTGG CCGGGCGCCG GGTCCAGCGC AGACCAGGAC 
GACGAGGAAG GAGTGGTAAA ATTTCAGCCT TCCCTTTGGC CTTGGGACTC AGTGAGGAAC 
AATTTGAGAA GTGCCCTGAC AGAGATGTGT GTTCTCTATG ATGTTCTCAG TATTGTTAGG 
GATAAAAAAT TTATGACTCT TGATCCTGTC TCTCAGGATG CACTTCCTCC AAAACAGAAT 
CCTCAGACGT TGCAATTGAT ATCTAAAAAG AAGTCACTTG CTGGAGCAGC ACAAATCTTA 
TTGAAGGGGG CAGAAAGACT GACTAAATCA GTTACCGAAA ACCAAGAAAA CAAGCTACAA 
AGAGACTTCA ATTCTGAGCT TTTGCGATTA CGGCAACACT GGAAACTTCG AAAAGTTGGA 
GATAAAATTC TCGGAGATCT GAGCTACAGA AGTGCAGGAT CTCTCTTTCC TCATCATGGT 
ACATTTGAAG TAATAAAGAA TACAGATCTC GATCTGGATA AAAAGATACC TGAAGATTAC 
TGTCCTCTTG ATGTCCAAAT TCCTAGTGAT TTAGAGGGGT CTGCATATAT CAAGGTTTCA 
ATACAAAAAC AGGCTCCAGA TATAGGTGAC CTCGGCACAG TTAACCTCTT CAAACGACCT 
TTGCCCAAAT CCAAACCAGG TTCCCCACAT TGGCAGACAA AATTAGAAGC GGCACAGAAT 
GTTCTCTTAT GTAAAGAAAT TTTTGCACAG CTCTCTCGGG AAGCTGTTCA AATTAAATCA 
CAAGTCCCTC ACATTGTGGT GAAAAACCAG ATTATCTCTC AGCCCTTTCC GAGCTTGCAG 
TTATCTATTT CTTTGTGCCA TTCCTCAAAT GATAAGAAAT CCCAAAAATT TGCTACTGAG 
AAGCAATGTC CGGAGGACCA CCTTTATGTC CTAGAGCATA ATTTGCATCT ACTGATTAGA 
GAGTTTCATA AACAGACCTT GAGTTCCATC ATGATGCCTC ATCCAGCAAG TGCACCTTTT 
GGCCACAAGA GAATGAGACT TTCGGGTCCT CAAGCTTTTG ATAAAAATGA AATTAATTCA 
TTACAGTCCA GTGAAGGGCT TCTGGAAAAA ATAATTAAAC AAGCAAAGCA TATTTTTCTA 
AGGAGTAGAG CTGCTGCAAC CATTGACAGC TTAGCAAGCC GAATTGAGGA TCCTCAGATA 
CAGGCTCATT GGTCAAATAT CAATGATGTT TATGAATCTA GTGTGAAAGT TTTAATCACA 
TCACAAGGCT ATGAACAAAT ATGCAAGTCC ATTCAACTGC AATTGAATAT TGGAGTTGAG 
CAGATTCGAG TTGTACATAG AGATGGAAGA GTAATTACAC TGTCTTATCA GGAGCAGGAG 
CTACAGGATT TTCTTCTGTC TCAGATGTCA CAGCACCAGG TACATGCAGT TCAGCAACTC 
GCCAAGGTTA TGGGCTGGCA AGTACTGAGC TTCAGTAATC ATGTGGGACT TGGACCTATA 
GAGAGCATTG GTAATGCATC TGCCATCACG GTGGCCTCCC CAAGTGGTGA CTATGCTATT 
TCAGTTCGTA ATGGACCTGA AAGTGGCAGC AAGATTATGG TTCAGTTTCC TCGTAACCAA 
TGTAAAGACC TTCCAAAAAG TGATGTTTTA CAAGATAACA AATGGAGTCA TCTTCGTGGG 
CCATTCAAAG AAGTTCAGTG GAATAAAATG GAAGGTCGAA ATTTTGTTTA TAAAATGGAG 
CTGCTTATGT CTGCACTTAG CCCTTGTCTA CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004259.3
CDS	233..2188
Translation	MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

Target ORF information:

RefSeq Version	NM_004268.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 17 (MED17), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004268.5

ORF Insert Sequence:

ATGTCCGGGG TGCGCGCAGT GCGGATCAGC ATCGAATCGG CCTGCGAGAA GCAGGTCCAT 
GAGGTGGGCC TGGATGGCAC CGAGACGTAC CTGCCCCCGC TGTCCATGTC GCAGAATCTG 
GCGCGTCTGG CCCAGCGGAT AGACTTCAGC CAGGGTTCGG GCTCCGAGGA GGAGGAGGCG 
GCGGGGACCG AGGGCGACGC GCAGGAGTGG CCGGGCGCCG GGTCCAGCGC AGACCAGGAC 
GACGAGGAAG GAGTGGTAAA ATTTCAGCCT TCCCTTTGGC CTTGGGACTC AGTGAGGAAC 
AATTTGAGAA GTGCCCTGAC AGAGATGTGT GTTCTCTATG ATGTTCTCAG TATTGTTAGG 
GATAAAAAAT TTATGACTCT TGATCCTGTC TCTCAGGATG CACTTCCTCC AAAACAGAAT 
CCTCAGACGT TGCAATTGAT ATCTAAAAAG AAGTCACTTG CTGGAGCAGC ACAAATCTTA 
TTGAAGGGGG CAGAAAGACT GACTAAATCA GTTACCGAAA ACCAAGAAAA CAAGCTACAA 
AGAGACTTCA ATTCTGAGCT TTTGCGATTA CGGCAACACT GGAAACTTCG AAAAGTTGGA 
GATAAAATTC TCGGAGATCT GAGCTACAGA AGTGCAGGAT CTCTCTTTCC TCATCATGGT 
ACATTTGAAG TAATAAAGAA TACAGATCTC GATCTGGATA AAAAGATACC TGAAGATTAC 
TGTCCTCTTG ATGTCCAAAT TCCTAGTGAT TTAGAGGGGT CTGCATATAT CAAGGTTTCA 
ATACAAAAAC AGGCTCCAGA TATAGGTGAC CTCGGCACAG TTAACCTCTT CAAACGACCT 
TTGCCCAAAT CCAAACCAGG TTCCCCACAT TGGCAGACAA AATTAGAAGC GGCACAGAAT 
GTTCTCTTAT GTAAAGAAAT TTTTGCACAG CTCTCTCGGG AAGCTGTTCA AATTAAATCA 
CAAGTCCCTC ACATTGTGGT GAAAAACCAG ATTATCTCTC AGCCCTTTCC GAGCTTGCAG 
TTATCTATTT CTTTGTGCCA TTCCTCAAAT GATAAGAAAT CCCAAAAATT TGCTACTGAG 
AAGCAATGTC CGGAGGACCA CCTTTATGTC CTAGAGCATA ATTTGCATCT ACTGATTAGA 
GAGTTTCATA AACAGACCTT GAGTTCCATC ATGATGCCTC ATCCAGCAAG TGCACCTTTT 
GGCCACAAGA GAATGAGACT TTCGGGTCCT CAAGCTTTTG ATAAAAATGA AATTAATTCA 
TTACAGTCCA GTGAAGGGCT TCTGGAAAAA ATAATTAAAC AAGCAAAGCA TATTTTTCTA 
AGGAGTAGAG CTGCTGCAAC CATTGACAGC TTAGCAAGCC GAATTGAGGA TCCTCAGATA 
CAGGCTCATT GGTCAAATAT CAATGATGTT TATGAATCTA GTGTGAAAGT TTTAATCACA 
TCACAAGGCT ATGAACAAAT ATGCAAGTCC ATTCAACTGC AATTGAATAT TGGAGTTGAG 
CAGATTCGAG TTGTACATAG AGATGGAAGA GTAATTACAC TGTCTTATCA GGAGCAGGAG 
CTACAGGATT TTCTTCTGTC TCAGATGTCA CAGCACCAGG TACATGCAGT TCAGCAACTC 
GCCAAGGTTA TGGGCTGGCA AGTACTGAGC TTCAGTAATC ATGTGGGACT TGGACCTATA 
GAGAGCATTG GTAATGCATC TGCCATCACG GTGGCCTCCC CAAGTGGTGA CTATGCTATT 
TCAGTTCGTA ATGGACCTGA AAGTGGCAGC AAGATTATGG TTCAGTTTCC TCGTAACCAA 
TGTAAAGACC TTCCAAAAAG TGATGTTTTA CAAGATAACA AATGGAGTCA TCTTCGTGGG 
CCATTCAAAG AAGTTCAGTG GAATAAAATG GAAGGTCGAA ATTTTGTTTA TAAAATGGAG 
CTGCTTATGT CTGCACTTAG CCCTTGTCTA CTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED17 ( XM_011543068.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541370.1 Homo sapiens mediator complex subunit 17 (MED17), transcript variant X1, mRNA.

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CloneID	OHu62823
Clone ID Related Accession (Same CDS sequence)	XM_011543068.2
Accession Version	XM_011543068.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 17 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGGGG TGCGCGCAGT GCGGATCAGC ATCGAATCGG CCTGCGAGAA GCAGGTCCAT 
GAGGTGGGCC TGGATGGCAC CGAGACGTAC CTGCCCCCGC TGTCCATGTC GCAGAATCTG 
GCGCGTCTGG CCCAGCGGAT AGACTTCAGC CAGGGTTCGG GCTCCGAGGA GGAGGAGGCG 
GCGGGGACCG AGGGCGACGC GCAGGAGTGG CCGGGCGCCG GGTCCAGCGC AGACCAGGAC 
GACGAGGAAG GAGTGGTAAA ATTTCAGCCT TCCCTTTGGC CTTGGGACTC AGTGAGGAAC 
AATTTGAGAA GTGCCCTGAC AGAGATGTGT GTTCTCTATG ATGTTCTCAG TATTGTTAGG 
GATAAAAAAT TTATGACTCT TGATCCTGTC TCTCAGGATG CACTTCCTCC AAAACAGAAT 
CCTCAGACGT TGCAATTGAT ATCTAAAAAG AAGTCACTTG CTGGAGCAGC ACAAATCTTA 
TTGAAGGGGG CAGAAAGACT GACTAAATCA GTTACCGAAA ACCAAGAAAA CAAGCTACAA 
AGAGACTTCA ATTCTGAGCT TTTGCGATTA CGGCAACACT GGAAACTTCG AAAAGTTGGA 
GATAAAATTC TCGGAGATCT GAGCTACAGA AGTGCAGGAT CTCTCTTTCC TCATCATGGT 
ACATTTGAAG TAATAAAGAA TACAGATCTC GATCTGGATA AAAAGATACC TGAAGATTAC 
TGTCCTCTTG ATGTCCAAAT TCCTAGTGAT TTAGAGGGGT CTGCATATAT CAAGGTTTCA 
ATACAAAAAC AGGCTCCAGA TATAGGTGAC CTCGGCACAG TTAACCTCTT CAAACGACCT 
TTGCCCAAAT CCAAACCAGG TTCCCCACAT TGGCAGACAA AATTAGAAGC GGCACAGAAT 
GTTCTCTTAT GTAAAGAAAT TTTTGCACAG CTCTCTCGGG AAGCTGTTCA AATTAAATCA 
CAAGTCCCTC ACATTGTGGT GAAAAACCAG ATTATCTCTC AGCCCTTTCC GAGCTTGCAG 
TTATCTATTT CTTTGTGCCA TTCCTCAAAT GATAAGAAAT CCCAAAAATT TGCTACTGAG 
AAGCAATGTC CGGAGGACCA CCTTTATGTC CTAGAGCATA ATTTGCATCT ACTGATTAGA 
GAGTTTCATA AACAGACCTT GAGTTCCATC ATGATGCCTC ATCCAGCAAG TGCACCTTTT 
GGCCACAAGA GAATGAGACT TTCGGGTCCT CAAGCTTTTG ATAAAAATGA AATTAATTCA 
TTACAGTCCA GTGAAGGGCT TCTGGAAAAA ATAATTAAAC AAGCAAAGCA TATTTTTCTA 
AGGAGTAGAG CTGCTGCAAC CATTGACAGC TTAGCAAGCC GAATTGAGGA TCCTCAGATA 
CAGGCTCATT GGTCAAATAT CAATGATGTT TATGAATCTA GTGTGAAAGT TTTAATCACA 
TCACAAGGCT ATGAACAAAT ATGCAAGTCC ATTCAACTGC AATTGAATAT TGGAGTTGAG 
CAGATTCGAG TTGTACATAG AGATGGAAGA GTAATTACAC TGTCTTATCA GGAGCAGGAG 
CTACAGGATT TTCTTCTGTC TCAGTTCGTA ATGGACCTGA AAGTGGCAGC AAGATTATGG 
TTCAGTTTCC TCGTAACCAA TGTAAAGACC TTCCAAAAAG TGATGTTTTA CAAGATAACA 
AATGGAGTCA TCTTCGTGGG CCATTCAAAG AAGTTCAGTG GAATAAAATG GAAGGTCGAA 
ATTTTGTTTA TAAAATGGAG CTGCTTATGT CTGCACTTAG CCCTTGTCTA CTATGATTTT 
TTCCAGATGT TTCCTAAAGA AGTTTCCAGA AACTTTGACT TGAAATGTTT GCAGATCAAC 
TATAAGCACA AAGAAGAGAT AACTTCCAAA AGAGTGCTGT TTTTAAAAAT AATAATTAGG 
AAATGTTTAT TTAGCACTTT CAAACTTTTC ACTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541370.1
CDS	235..2193
Translation	MSGVRAVRISIESACEKQVHEVGLDGTETYLPPLSMSQNLARLAQRIDFSQGSGSEEEEAAGTEGDAQEWPGAGSSADQDDEEGVVKFQPSLWPWDSVRNNLRSALTEMCVLYDVLSIVRDKKFMTLDPVSQDALPPKQNPQTLQLISKKKSLAGAAQILLKGAERLTKSVTENQENKLQRDFNSELLRLRQHWKLRKVGDKILGDLSYRSAGSLFPHHGTFEVIKNTDLDLDKKIPEDYCPLDVQIPSDLEGSAYIKVSIQKQAPDIGDLGTVNLFKRPLPKSKPGSPHWQTKLEAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQFVMDLKVAARLWFSFLVTNVKTFQKVMFYKITNGVIFVGHSKKFSGIKWKVEILFIKWSCLCLHLALVYYDFFQMFPKEVSRNFDLKCLQINYKHKEEITSKRVLFLKIIIRKCLFSTFKLFTL

Target ORF information:

RefSeq Version	XM_011543068.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 17 (MED17), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543068.2

ORF Insert Sequence:

ATGTCCGGGG TGCGCGCAGT GCGGATCAGC ATCGAATCGG CCTGCGAGAA GCAGGTCCAT 
GAGGTGGGCC TGGATGGCAC CGAGACGTAC CTGCCCCCGC TGTCCATGTC GCAGAATCTG 
GCGCGTCTGG CCCAGCGGAT AGACTTCAGC CAGGGTTCGG GCTCCGAGGA GGAGGAGGCG 
GCGGGGACCG AGGGCGACGC GCAGGAGTGG CCGGGCGCCG GGTCCAGCGC AGACCAGGAC 
GACGAGGAAG GAGTGGTAAA ATTTCAGCCT TCCCTTTGGC CTTGGGACTC AGTGAGGAAC 
AATTTGAGAA GTGCCCTGAC AGAGATGTGT GTTCTCTATG ATGTTCTCAG TATTGTTAGG 
GATAAAAAAT TTATGACTCT TGATCCTGTC TCTCAGGATG CACTTCCTCC AAAACAGAAT 
CCTCAGACGT TGCAATTGAT ATCTAAAAAG AAGTCACTTG CTGGAGCAGC ACAAATCTTA 
TTGAAGGGGG CAGAAAGACT GACTAAATCA GTTACCGAAA ACCAAGAAAA CAAGCTACAA 
AGAGACTTCA ATTCTGAGCT TTTGCGATTA CGGCAACACT GGAAACTTCG AAAAGTTGGA 
GATAAAATTC TCGGAGATCT GAGCTACAGA AGTGCAGGAT CTCTCTTTCC TCATCATGGT 
ACATTTGAAG TAATAAAGAA TACAGATCTC GATCTGGATA AAAAGATACC TGAAGATTAC 
TGTCCTCTTG ATGTCCAAAT TCCTAGTGAT TTAGAGGGGT CTGCATATAT CAAGGTTTCA 
ATACAAAAAC AGGCTCCAGA TATAGGTGAC CTCGGCACAG TTAACCTCTT CAAACGACCT 
TTGCCCAAAT CCAAACCAGG TTCCCCACAT TGGCAGACAA AATTAGAAGC GGCACAGAAT 
GTTCTCTTAT GTAAAGAAAT TTTTGCACAG CTCTCTCGGG AAGCTGTTCA AATTAAATCA 
CAAGTCCCTC ACATTGTGGT GAAAAACCAG ATTATCTCTC AGCCCTTTCC GAGCTTGCAG 
TTATCTATTT CTTTGTGCCA TTCCTCAAAT GATAAGAAAT CCCAAAAATT TGCTACTGAG 
AAGCAATGTC CGGAGGACCA CCTTTATGTC CTAGAGCATA ATTTGCATCT ACTGATTAGA 
GAGTTTCATA AACAGACCTT GAGTTCCATC ATGATGCCTC ATCCAGCAAG TGCACCTTTT 
GGCCACAAGA GAATGAGACT TTCGGGTCCT CAAGCTTTTG ATAAAAATGA AATTAATTCA 
TTACAGTCCA GTGAAGGGCT TCTGGAAAAA ATAATTAAAC AAGCAAAGCA TATTTTTCTA 
AGGAGTAGAG CTGCTGCAAC CATTGACAGC TTAGCAAGCC GAATTGAGGA TCCTCAGATA 
CAGGCTCATT GGTCAAATAT CAATGATGTT TATGAATCTA GTGTGAAAGT TTTAATCACA 
TCACAAGGCT ATGAACAAAT ATGCAAGTCC ATTCAACTGC AATTGAATAT TGGAGTTGAG 
CAGATTCGAG TTGTACATAG AGATGGAAGA GTAATTACAC TGTCTTATCA GGAGCAGGAG 
CTACAGGATT TTCTTCTGTC TCAGTTCGTA ATGGACCTGA AAGTGGCAGC AAGATTATGG 
TTCAGTTTCC TCGTAACCAA TGTAAAGACC TTCCAAAAAG TGATGTTTTA CAAGATAACA 
AATGGAGTCA TCTTCGTGGG CCATTCAAAG AAGTTCAGTG GAATAAAATG GAAGGTCGAA 
ATTTTGTTTA TAAAATGGAG CTGCTTATGT CTGCACTTAG CCCTTGTCTA CTATGATTTT 
TTCCAGATGT TTCCTAAAGA AGTTTCCAGA AACTTTGACT TGAAATGTTT GCAGATCAAC 
TATAAGCACA AAGAAGAGAT AACTTCCAAA AGAGTGCTGT TTTTAAAAAT AATAATTAGG 
AAATGTTTAT TTAGCACTTT CAAACTTTTC ACTTTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Human mediator MED17 subunit plays essential roles in gene regulation by associating with the transcription and DNA repair machineries.
Genes to cells : devoted to molecular & cellular mechanisms20(3)191-202(2015 Mar)
Kikuchi Y,Umemura H,Nishitani S,Iida S,Fukasawa R,Hayashi H,Hirose Y,Tanaka A,Sugasawa K,Ohkuma Y

Hepatic TRAP80 selectively regulates lipogenic activity of liver X receptor.
The Journal of clinical investigation125(1)183-93(2015 Jan)
Kim GH,Oh GS,Yoon J,Lee GG,Lee KU,Kim SW

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

Mediator and post-recruitment regulation of RNA polymerase II.
Transcription2(1)28-31(2011 Jan-Feb)
Knuesel MT,Taatjes DJ

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MED17 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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