ECEL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ECEL1
Entrez Gene ID	9427
Full Name	endothelin converting enzyme like 1
Synonyms	DA5D,DINE,ECEX,XCE
General protein information	Preferred Names endothelin converting enzyme like 1 Names endothelin-converting enzyme-like 1 X converting enzyme damage induced neuronal endopeptidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q37.1
Summary	This gene encodes a member of the M13 family of endopeptidases. Members of this family are zinc-containing type II integral-membrane proteins that are important regulators of neuropeptide and peptide hormone activity. Mutations in this gene are associated with autosomal recessive distal arthrogryposis, type 5D. This gene has multiple pseudogenes on chromosome 2. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014].
Disorder MIM:	605896
Disorder Html:	Arthrogryposis, distal, type 5D, 615065 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001290787.2	NP_001277716.1	endothelin-converting enzyme-like 1 isoform 2
NM_004826.4	NP_004817.2	endothelin-converting enzyme-like 1 isoform 1
NM_001290787.1	NP_001277716.1	endothelin-converting enzyme-like 1 isoform 2
NM_004826.3	NP_004817.2	endothelin-converting enzyme-like 1 isoform 1

Pathways from BioSystems

Homologies

	ECEL1	XP_001117070.2
Rattus norvegicus (Norway rat)	Ecel1	NP_068544.1
Mus musculus (house mouse)	Ecel1	NP_001264854.1
Gallus gallus (chicken)	ECEL1	XP_422744.3
Pan troglodytes (chimpanzee)	ECEL1	XP_516151.3
Bos taurus (cattle)	ECEL1	XP_005203103.1
Xenopus tropicalis (tropical clawed frog)	ecel1	XP_002937386.2
Homo sapiens (human)	ECEL1	NP_004817.2
Canis lupus familiaris (dog)	ECEL1	XP_543287.2

Gene Ontology
Bibliography

Related articles in PubMed

ECEL1 mutation causes fetal arthrogryposis multiplex congenita.
Dohrn N, Le VQ, Petersen A, Skovbo P, Pedersen IS, Ernst A, Krarup H, Petersen MB
American journal of medical genetics. Part A167A(4)731-43(2015 Apr)

The ECEL1-related strabismus phenotype is consistent with congenital cranial dysinnervation disorder.
Khan AO, Shaheen R, Alkuraya FS
Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus18(4)362-7(2014 Aug)

Identification of three novel ECEL1 mutations in three families with distal arthrogryposis type 5D.
Shaheen R, Al-Owain M, Khan AO, Zaki MS, Hossni HA, Al-Tassan R, Eyaid W, Alkuraya FS
Clinical genetics85(6)568-72(2014 Jun)

Mutations in ECEL1 cause distal arthrogryposis type 5D.
McMillin MJ, Below JE, Shively KM, Beck AE, Gildersleeve HI, Pinner J, Gogola GR, Hecht JT, Grange DK, Harris DJ, Earl DL, Jagadeesh S, Mehta SG, Robertson SP, Swanson JM, Faustman EM, Mefford HC, Shendure J, Nickerson DA, Bamshad MJ,
American journal of human genetics92(1)150-6(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutation of a conserved residue in ECEL1 is linked with fetal arthrogryposis multiplex congenita.
Title: ECEL1 mutation causes fetal arthrogryposis multiplex congenita.

Our clinical findings are consistent with recessive ECEL1 mutations causing variably penetrant orbital dysinnervation phenotypes (ptosis and/or complex strabismus with abnormal synkinesis)
Title: The ECEL1-related strabismus phenotype is consistent with congenital cranial dysinnervation disorder.

A novel missense c.1819G>A mutation (G607S) in the ECEL1 gene has been identified in a consanguineous pedigree of Turkish origin presenting with congenital contracture syndromes.
Title: Expanding the phenotypic spectrum of ECEL1-related congenital contracture syndromes.

Three novel ECEL1 mutations have been identified in consanguineous pedigrees of Saudi Arabian origin presenting with distal arthrogryposis type 5D.
Title: Identification of three novel ECEL1 mutations in three families with distal arthrogryposis type 5D.

Mutations in ECEL1 cause distal arthrogryposis type 5D.
Title: Mutations in ECEL1 cause distal arthrogryposis type 5D.

The following ECEL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ECEL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23860	NM_004826.4 Latest version!	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu24002	NM_001290787.2 Latest version!	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu24002	NM_001290787.1	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu23860	NM_004826.3	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ECEL1 ( NM_004826.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004817.2 Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23860
Clone ID Related Accession (Same CDS sequence)	NM_004826.4 , NM_004826.3
Accession Version	NM_004826.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme-like 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092165.4 and BC050453.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004826.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050453.1, AY358923.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304546.6/ ENSP00000302051.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TCCACGTGGC TGCTCCCCCC ACAGGCGCTC AATGCCTACT ATCTACCCAA CAAGAACCAG 
ATGGTGTTCC CCGCGGGCAT CCTGCAGCCC ACCCTGTACG ACCCTGACTT CCCACAGTCT 
CTCAACTACG GGGGCATCGG CACCATCATT GGACATGAGC TGACCCACGG CTACGACGAC 
TGGGGGGGCC AGTATGACCG CTCAGGGAAC CTGCTGCACT GGTGGACGGA GGCCTCCTAC 
AGCCGCTTCC TGCGAAAGGC TGAGTGCATC GTCCGTCTCT ATGACAACTT CACTGTCTAC 
AACCAGCGGG TGAACGGGAA ACACACGCTT GGGGAGAACA TCGCAGATAT GGGCGGCCTC 
AAGCTGGCCT ACCACGCCTA TCAGAAGTGG GTGCGGGAGC ACGGCCCAGA GCACCCACTT 
CCCCGGCTCA AGTACACACA TGACCAGCTC TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGGC GGTCGCAGTC CATCTACCTG CAGGTGCTGA CTGACAAGCA TGCCCCTGAG 
CACTACAGGG TGCTGGGCAG TGTGTCCCAG TTTGAGGAGT TTGGCCGGGC TTTCCACTGT 
CCCAAGGACT CACCCATGAA CCCTGCCCAC AAGTGTTCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004817.2
CDS	218..2545
Translation	MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEHDDLRRDVSSMYNKVTLGQLQKITPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREALHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPLPRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW

Target ORF information:

RefSeq Version	NM_004826.4
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004826.4

ORF Insert Sequence:

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TCCACGTGGC TGCTCCCCCC ACAGGCGCTC AATGCCTACT ATCTACCCAA CAAGAACCAG 
ATGGTGTTCC CCGCGGGCAT CCTGCAGCCC ACCCTGTACG ACCCTGACTT CCCACAGTCT 
CTCAACTACG GGGGCATCGG CACCATCATT GGACATGAGC TGACCCACGG CTACGACGAC 
TGGGGGGGCC AGTATGACCG CTCAGGGAAC CTGCTGCACT GGTGGACGGA GGCCTCCTAC 
AGCCGCTTCC TGCGAAAGGC TGAGTGCATC GTCCGTCTCT ATGACAACTT CACTGTCTAC 
AACCAGCGGG TGAACGGGAA ACACACGCTT GGGGAGAACA TCGCAGATAT GGGCGGCCTC 
AAGCTGGCCT ACCACGCCTA TCAGAAGTGG GTGCGGGAGC ACGGCCCAGA GCACCCACTT 
CCCCGGCTCA AGTACACACA TGACCAGCTC TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGGC GGTCGCAGTC CATCTACCTG CAGGTGCTGA CTGACAAGCA TGCCCCTGAG 
CACTACAGGG TGCTGGGCAG TGTGTCCCAG TTTGAGGAGT TTGGCCGGGC TTTCCACTGT 
CCCAAGGACT CACCCATGAA CCCTGCCCAC AAGTGTTCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECEL1 ( NM_004826.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004817.2 Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23860
Clone ID Related Accession (Same CDS sequence)	NM_004826.4 , NM_004826.3
Accession Version	NM_004826.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme-like 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092165.4 and BC050453.1. This sequence is a reference standard in the RefSeqGene project. On Mar 20, 2014 this sequence version replaced NM_004826.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050453.1, Y16187.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TCCACGTGGC TGCTCCCCCC ACAGGCGCTC AATGCCTACT ATCTACCCAA CAAGAACCAG 
ATGGTGTTCC CCGCGGGCAT CCTGCAGCCC ACCCTGTACG ACCCTGACTT CCCACAGTCT 
CTCAACTACG GGGGCATCGG CACCATCATT GGACATGAGC TGACCCACGG CTACGACGAC 
TGGGGGGGCC AGTATGACCG CTCAGGGAAC CTGCTGCACT GGTGGACGGA GGCCTCCTAC 
AGCCGCTTCC TGCGAAAGGC TGAGTGCATC GTCCGTCTCT ATGACAACTT CACTGTCTAC 
AACCAGCGGG TGAACGGGAA ACACACGCTT GGGGAGAACA TCGCAGATAT GGGCGGCCTC 
AAGCTGGCCT ACCACGCCTA TCAGAAGTGG GTGCGGGAGC ACGGCCCAGA GCACCCACTT 
CCCCGGCTCA AGTACACACA TGACCAGCTC TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGGC GGTCGCAGTC CATCTACCTG CAGGTGCTGA CTGACAAGCA TGCCCCTGAG 
CACTACAGGG TGCTGGGCAG TGTGTCCCAG TTTGAGGAGT TTGGCCGGGC TTTCCACTGT 
CCCAAGGACT CACCCATGAA CCCTGCCCAC AAGTGTTCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004817.2
CDS	243..2570
Translation	MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEHDDLRRDVSSMYNKVTLGQLQKITPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREALHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKSTWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPLPRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW

Target ORF information:

RefSeq Version	NM_004826.3
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004826.3

ORF Insert Sequence:

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TCCACGTGGC TGCTCCCCCC ACAGGCGCTC AATGCCTACT ATCTACCCAA CAAGAACCAG 
ATGGTGTTCC CCGCGGGCAT CCTGCAGCCC ACCCTGTACG ACCCTGACTT CCCACAGTCT 
CTCAACTACG GGGGCATCGG CACCATCATT GGACATGAGC TGACCCACGG CTACGACGAC 
TGGGGGGGCC AGTATGACCG CTCAGGGAAC CTGCTGCACT GGTGGACGGA GGCCTCCTAC 
AGCCGCTTCC TGCGAAAGGC TGAGTGCATC GTCCGTCTCT ATGACAACTT CACTGTCTAC 
AACCAGCGGG TGAACGGGAA ACACACGCTT GGGGAGAACA TCGCAGATAT GGGCGGCCTC 
AAGCTGGCCT ACCACGCCTA TCAGAAGTGG GTGCGGGAGC ACGGCCCAGA GCACCCACTT 
CCCCGGCTCA AGTACACACA TGACCAGCTC TTCTTCATTG CCTTTGCCCA GAACTGGTGC 
ATCAAGCGGC GGTCGCAGTC CATCTACCTG CAGGTGCTGA CTGACAAGCA TGCCCCTGAG 
CACTACAGGG TGCTGGGCAG TGTGTCCCAG TTTGAGGAGT TTGGCCGGGC TTTCCACTGT 
CCCAAGGACT CACCCATGAA CCCTGCCCAC AAGTGTTCCG TGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECEL1 ( NM_001290787.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277716.1 Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24002
Clone ID Related Accession (Same CDS sequence)	NM_001290787.2 , NM_001290787.1
Accession Version	NM_001290787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme-like 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092165.4, BC050453.1 and DQ114476.1. On May 31, 2019 this sequence version replaced NM_001290787.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ114476.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TGGCTGCTCC CCCCACAGGC GCTCAATGCC TACTATCTAC CCAACAAGAA CCAGATGGTG 
TTCCCCGCGG GCATCCTGCA GCCCACCCTG TACGACCCTG ACTTCCCACA GTCTCTCAAC 
TACGGGGGCA TCGGCACCAT CATTGGACAT GAGCTGACCC ACGGCTACGA CGACTGGGGG 
GGCCAGTATG ACCGCTCAGG GAACCTGCTG CACTGGTGGA CGGAGGCCTC CTACAGCCGC 
TTCCTGCGAA AGGCTGAGTG CATCGTCCGT CTCTATGACA ACTTCACTGT CTACAACCAG 
CGGGTGAACG GGAAACACAC GCTTGGGGAG AACATCGCAG ATATGGGCGG CCTCAAGCTG 
GCCTACCACG CCTATCAGAA GTGGGTGCGG GAGCACGGCC CAGAGCACCC ACTTCCCCGG 
CTCAAGTACA CACATGACCA GCTCTTCTTC ATTGCCTTTG CCCAGAACTG GTGCATCAAG 
CGGCGGTCGC AGTCCATCTA CCTGCAGGTG CTGACTGACA AGCATGCCCC TGAGCACTAC 
AGGGTGCTGG GCAGTGTGTC CCAGTTTGAG GAGTTTGGCC GGGCTTTCCA CTGTCCCAAG 
GACTCACCCA TGAACCCTGC CCACAAGTGT TCCGTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277716.1
CDS	218..2539
Translation	MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEHDDLRRDVSSMYNKVTLGQLQKITPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREALHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPLPRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW

Target ORF information:

RefSeq Version	NM_001290787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290787.2

ORF Insert Sequence:

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TGGCTGCTCC CCCCACAGGC GCTCAATGCC TACTATCTAC CCAACAAGAA CCAGATGGTG 
TTCCCCGCGG GCATCCTGCA GCCCACCCTG TACGACCCTG ACTTCCCACA GTCTCTCAAC 
TACGGGGGCA TCGGCACCAT CATTGGACAT GAGCTGACCC ACGGCTACGA CGACTGGGGG 
GGCCAGTATG ACCGCTCAGG GAACCTGCTG CACTGGTGGA CGGAGGCCTC CTACAGCCGC 
TTCCTGCGAA AGGCTGAGTG CATCGTCCGT CTCTATGACA ACTTCACTGT CTACAACCAG 
CGGGTGAACG GGAAACACAC GCTTGGGGAG AACATCGCAG ATATGGGCGG CCTCAAGCTG 
GCCTACCACG CCTATCAGAA GTGGGTGCGG GAGCACGGCC CAGAGCACCC ACTTCCCCGG 
CTCAAGTACA CACATGACCA GCTCTTCTTC ATTGCCTTTG CCCAGAACTG GTGCATCAAG 
CGGCGGTCGC AGTCCATCTA CCTGCAGGTG CTGACTGACA AGCATGCCCC TGAGCACTAC 
AGGGTGCTGG GCAGTGTGTC CCAGTTTGAG GAGTTTGGCC GGGCTTTCCA CTGTCCCAAG 
GACTCACCCA TGAACCCTGC CCACAAGTGT TCCGTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ECEL1 ( NM_001290787.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277716.1 Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu24002
Clone ID Related Accession (Same CDS sequence)	NM_001290787.2 , NM_001290787.1
Accession Version	NM_001290787.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	endothelin-converting enzyme-like 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092165.4, BC050453.1 and DQ114476.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. This results in a shorter protein (isoform 2) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: DQ114476.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TGGCTGCTCC CCCCACAGGC GCTCAATGCC TACTATCTAC CCAACAAGAA CCAGATGGTG 
TTCCCCGCGG GCATCCTGCA GCCCACCCTG TACGACCCTG ACTTCCCACA GTCTCTCAAC 
TACGGGGGCA TCGGCACCAT CATTGGACAT GAGCTGACCC ACGGCTACGA CGACTGGGGG 
GGCCAGTATG ACCGCTCAGG GAACCTGCTG CACTGGTGGA CGGAGGCCTC CTACAGCCGC 
TTCCTGCGAA AGGCTGAGTG CATCGTCCGT CTCTATGACA ACTTCACTGT CTACAACCAG 
CGGGTGAACG GGAAACACAC GCTTGGGGAG AACATCGCAG ATATGGGCGG CCTCAAGCTG 
GCCTACCACG CCTATCAGAA GTGGGTGCGG GAGCACGGCC CAGAGCACCC ACTTCCCCGG 
CTCAAGTACA CACATGACCA GCTCTTCTTC ATTGCCTTTG CCCAGAACTG GTGCATCAAG 
CGGCGGTCGC AGTCCATCTA CCTGCAGGTG CTGACTGACA AGCATGCCCC TGAGCACTAC 
AGGGTGCTGG GCAGTGTGTC CCAGTTTGAG GAGTTTGGCC GGGCTTTCCA CTGTCCCAAG 
GACTCACCCA TGAACCCTGC CCACAAGTGT TCCGTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277716.1
CDS	243..2564
Translation	MEPPYSLTAHYDEFQEVKYVSRCGAGGARGASLPPGFPLGAARSATGARSGLPRWNRREVCLLSGLVFAAGLCAILAAMLALKYLGPVAAGGGACPEGCPERKAFARAARFLAANLDASIDPCQDFYSFACGGWLRRHAIPDDKLTYGTIAAIGEQNEERLRRLLARPGGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCGGWDLGGAEERPGVAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQDEDSEKILAAYRVFMERVLSLLGADAVEQKAQEILQVEQQLANITVSEHDDLRRDVSSMYNKVTLGQLQKITPHLRWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPHRVLHNYLVWRVVVVLSEHLSPPFREALHELAQEMEGSDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDWMDAETRAAARAKLQYMMVMVGYPDFLLKPDAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDKWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGNLLHWWTEASYSRFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYHAYQKWVREHGPEHPLPRLKYTHDQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPAHKCSVW

Target ORF information:

RefSeq Version	NM_001290787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens endothelin converting enzyme like 1 (ECEL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290787.1

ORF Insert Sequence:

ATGGAGCCCC CGTATTCGCT GACGGCGCAC TACGATGAGT TCCAAGAGGT CAAGTACGTG 
AGCCGCTGCG GCGCGGGGGG CGCGCGCGGG GCCTCCCTGC CCCCGGGCTT CCCGTTGGGC 
GCTGCGCGCA GCGCCACCGG GGCCCGGTCC GGGCTGCCGC GCTGGAACCG GCGCGAGGTG 
TGCCTGCTGT CGGGGCTGGT GTTCGCCGCC GGCCTCTGCG CCATTCTGGC GGCTATGCTG 
GCCCTCAAGT ACCTGGGCCC GGTCGCGGCC GGCGGCGGCG CCTGTCCCGA GGGCTGCCCT 
GAGCGCAAGG CCTTCGCGCG CGCCGCTCGC TTCCTGGCCG CCAACCTGGA CGCCAGCATC 
GACCCATGCC AGGACTTCTA CTCGTTCGCC TGCGGCGGTT GGCTGCGGCG CCACGCCATC 
CCCGACGACA AGCTCACCTA TGGCACCATC GCGGCCATCG GCGAGCAAAA CGAGGAGCGC 
CTACGGCGCC TGCTGGCGCG GCCCGGGGGT GGGCCTGGCG GCGCGGCCCA GCGCAAGGTG 
CGCGCCTTCT TCCGCTCGTG CCTCGACATG CGCGAGATCG AGCGACTGGG CCCGCGACCC 
ATGCTAGAGG TCATCGAGGA CTGCGGGGGC TGGGACCTGG GCGGCGCGGA GGAGCGTCCG 
GGGGTCGCGG CGCGATGGGA CCTCAACCGG CTGCTGTACA AGGCGCAGGG CGTGTACAGC 
GCCGCCGCGC TCTTCTCGCT CACGGTCAGC CTGGACGACA GGAACTCCTC GCGCTACGTC 
ATCCGCATTG ACCAGGATGG GCTCACCCTG CCAGAGAGGA CCCTGTACCT CGCTCAGGAT 
GAGGACAGTG AGAAGATCCT GGCAGCATAC AGGGTGTTCA TGGAGCGAGT GCTCAGCCTC 
CTGGGTGCAG ACGCTGTGGA ACAGAAGGCC CAAGAGATCC TGCAAGTGGA GCAGCAGCTG 
GCCAACATCA CTGTGTCAGA GCATGACGAC CTACGGCGAG ATGTCAGCTC CATGTACAAC 
AAGGTGACGC TGGGGCAGCT GCAGAAGATC ACCCCCCACT TGCGGTGGAA GTGGCTGCTA 
GACCAGATCT TCCAGGAGGA CTTCTCAGAG GAAGAGGAGG TGGTGCTGCT GGCGACAGAC 
TACATGCAGC AGGTGTCGCA GCTCATCCGC TCCACACCCC ACCGGGTCCT GCACAACTAC 
CTGGTGTGGC GCGTGGTGGT GGTCCTGAGT GAACACCTGT CCCCGCCATT CCGTGAGGCA 
CTGCACGAGC TGGCACAGGA GATGGAGGGC AGCGACAAGC CACAGGAGCT GGCCCGGGTC 
TGCTTGGGCC AGGCCAATCG CCACTTTGGC ATGGCGCTTG GCGCCCTCTT TGTACATGAG 
CACTTCTCAG CTGCCAGCAA AGCCAAGGTG CAGCAGCTAG TGGAAGACAT CAAGTACATC 
CTGGGCCAGC GCCTGGAGGA GCTGGACTGG ATGGACGCCG AGACCAGGGC TGCTGCTCGG 
GCCAAGCTCC AGTACATGAT GGTGATGGTC GGCTACCCGG ACTTCCTGCT GAAACCCGAT 
GCTGTGGACA AGGAGTATGA GTTTGAGGTC CATGAGAAGA CCTACTTCAA GAACATCTTG 
AACAGCATCC GCTTCAGCAT CCAGCTCTCA GTTAAGAAGA TTCGGCAGGA GGTGGACAAG 
TGGCTGCTCC CCCCACAGGC GCTCAATGCC TACTATCTAC CCAACAAGAA CCAGATGGTG 
TTCCCCGCGG GCATCCTGCA GCCCACCCTG TACGACCCTG ACTTCCCACA GTCTCTCAAC 
TACGGGGGCA TCGGCACCAT CATTGGACAT GAGCTGACCC ACGGCTACGA CGACTGGGGG 
GGCCAGTATG ACCGCTCAGG GAACCTGCTG CACTGGTGGA CGGAGGCCTC CTACAGCCGC 
TTCCTGCGAA AGGCTGAGTG CATCGTCCGT CTCTATGACA ACTTCACTGT CTACAACCAG 
CGGGTGAACG GGAAACACAC GCTTGGGGAG AACATCGCAG ATATGGGCGG CCTCAAGCTG 
GCCTACCACG CCTATCAGAA GTGGGTGCGG GAGCACGGCC CAGAGCACCC ACTTCCCCGG 
CTCAAGTACA CACATGACCA GCTCTTCTTC ATTGCCTTTG CCCAGAACTG GTGCATCAAG 
CGGCGGTCGC AGTCCATCTA CCTGCAGGTG CTGACTGACA AGCATGCCCC TGAGCACTAC 
AGGGTGCTGG GCAGTGTGTC CCAGTTTGAG GAGTTTGGCC GGGCTTTCCA CTGTCCCAAG 
GACTCACCCA TGAACCCTGC CCACAAGTGT TCCGTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ECEL1 mutation causes fetal arthrogryposis multiplex congenita.
American journal of medical genetics. Part A167A(4)731-43(2015 Apr)
Dohrn N,Le VQ,Petersen A,Skovbo P,Pedersen IS,Ernst A,Krarup H,Petersen MB

The ECEL1-related strabismus phenotype is consistent with congenital cranial dysinnervation disorder.
Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus18(4)362-7(2014 Aug)
Khan AO,Shaheen R,Alkuraya FS

Identification of three novel ECEL1 mutations in three families with distal arthrogryposis type 5D.
Clinical genetics85(6)568-72(2014 Jun)
Shaheen R,Al-Owain M,Khan AO,Zaki MS,Hossni HA,Al-Tassan R,Eyaid W,Alkuraya FS

Mutations in ECEL1 cause distal arthrogryposis type 5D.
American journal of human genetics92(1)150-6(2013 Jan)
McMillin MJ,Below JE,Shively KM,Beck AE,Gildersleeve HI,Pinner J,Gogola GR,Hecht JT,Grange DK,Harris DJ,Earl DL,Jagadeesh S,Mehta SG,Robertson SP,Swanson JM,Faustman EM,Mefford HC,Shendure J,Nickerson DA,Bamshad MJ,

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ECEL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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