CYP7B1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYP7B1
Entrez Gene ID	9420
Full Name	cytochrome P450 family 7 subfamily B member 1
Synonyms	CBAS3,CP7B,SPG5A
General protein information	Preferred Names cytochrome P450 family 7 subfamily B member 1 Names 25-hydroxycholesterol 7-alpha-hydroxylase cytochrome P450 7B1 cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1 oxysterol 7-alpha-hydroxylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q12.3
Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis. Mutations in this gene have been associated with hereditary spastic paraplegia (SPG5 or HSP), an autosomal recessive disorder. [provided by RefSeq, Apr 2016].
Disorder MIM:	603711
Disorder Html:	Bile acid synthesis defect, congenital, 3, 613812 (3); Spastic paraplegia 5A, autosomal recessive, 270800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004820.3	NP_004811.1	25-hydroxycholesterol 7-alpha-hydroxylase
NM_001324112.2	NP_001311041.1	cytochrome P450 7B1 isoform 2
NM_004820.4	NP_004811.1	25-hydroxycholesterol 7-alpha-hydroxylase isoform 1
NM_001324112.1	NP_001311041.1	25-hydroxycholesterol 7-alpha-hydroxylase isoform 2
NM_004820.5	NP_004811.1	cytochrome P450 7B1 isoform 1
XM_017014002.1	XP_016869491.1	cytochrome P450 7B1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC570455	XP_699028.2
Canis lupus familiaris (dog)	CYP7B1	XP_005638074.1
Bos taurus (cattle)	CYP7B1	XP_002692741.1
Gallus gallus (chicken)	CYP7B1	XP_418276.4
Rattus norvegicus (Norway rat)	Cyp7b1	NP_062011.1
Homo sapiens (human)	CYP7B1	NP_004811.1
	LOC710188	XP_001098766.2
Xenopus tropicalis (tropical clawed frog)	cyp7b1	NP_001120511.1
Pan troglodytes (chimpanzee)	CYP7B1	XP_001159731.1
Mus musculus (house mouse)	Cyp7b1	NP_031851.3

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
Xi XP, Zhuang J, Teng MJ, Xia LJ, Yang MY, Liu QG, Chen JB
International journal of molecular medicine38(2)499-506(2016 Aug)

Hereditary spastic paraplegia in Greece: characterisation of a previously unexplored population using next-generation sequencing.
Lynch DS, Koutsis G, Tucci A, Panas M, Baklou M, Breza M, Karadima G, Houlden H
European journal of human genetics : EJHG24(6)857-63(2016 06)

Mutational analysis of the CYP7B1, PNPLA6 and C19orf12 genes in autosomal recessive hereditary spastic paraplegia.
Schubert SF, Hoffjan S, Dekomien G
Molecular and cellular probes30(1)53-5(2016 Feb)

Variants in the CYP7B1 gene region do not affect natural resistance to HIV-1 infection.
Sironi M, Biasin M, Pontremoli C, Cagliani R, Saulle I, Trabattoni D, Vichi F, Lo Caputo S, Mazzotta F, Aguilar-Jimenez W, Rugeles MT, Cedeno S, Sanchez J, Brander C, Clerici M
Retrovirology1280(2015 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
Title: MicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.

SPG11 and CYP7B1 were the most common cause of autosomal recessive hereditary spastic paraplegia in Greece.
Title: Hereditary spastic paraplegia in Greece: characterisation of a previously unexplored population using next-generation sequencing.

The two novel variants cosegregated with pyramidal signs and autoimmune diseases suggesting that they might be susceptibility factors.
Title: SPG5 and multiple sclerosis: clinical and genetic overlap?

Data indicate two novel homozygous mutations (one frameshift and one missense mutation) detected in CYP7B1 (SPG5A), while no disease-causing mutation was identified for PNPLA6 (SPG39) and C19orf12 (SPG43).
Title: Mutational analysis of the CYP7B1, PNPLA6 and C19orf12 genes in autosomal recessive hereditary spastic paraplegia.

Data indicated that the two GWAS-defined variants in the CYP7B1 region do not strongly influence HIV-1 infection susceptibility.
Title: Variants in the CYP7B1 gene region do not affect natural resistance to HIV-1 infection.

The following CYP7B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP7B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27221	NM_004820.5 Latest version!	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu101740	NM_001324112.2 Latest version!	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu98308	XM_017014002.1 Latest version!	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu27221	NM_004820.3	Homo sapiens cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu101740	NM_001324112.1	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu27221	NM_004820.4	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYP7B1 ( NM_004820.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004811.1 Homo sapiens cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mRNA.

Price & Availability↑

CloneID	OHu27221
Clone ID Related Accession (Same CDS sequence)	NM_004820.3 , NM_004820.4 , NM_004820.5
Accession Version	NM_004820.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	25-hydroxycholesterol 7-alpha-hydroxylase
Comment

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGGAGTTTA GATATGATCG TTTTATAGAA 
GATGGTAAGA AGAAAACCAC CTTTTTCAAA AGAGGGAAAA AGCTGAAGTG TTACCTAATG 
CCGTTTGGAA CTGGAACCAG CAAATGTCCA GGCCGATTTT TTGCACTTAT GGAAATAAAA 
CAATTGTTGG TTATACTTTT AACTTATTTT GATTTAGAAA TAATTGATGA TAAGCCCATA 
GGACTAAACT ACAGCCGCTT GTTGTTTGGT ATTCAGTATC CAGATTCTGA TGTTTTATTT 
AGATACAAAG TGAAATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_004811.1

Translation

MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIEDGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPIGLNYSRLLFGIQYPDSDVLFRYKVKS

Target ORF information:

RefSeq Version	NM_004820.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens cytochrome P450, family 7, subfamily B, polypeptide 1 (CYP7B1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004820.3

ORF Insert Sequence:

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGGAGTTTA GATATGATCG TTTTATAGAA 
GATGGTAAGA AGAAAACCAC CTTTTTCAAA AGAGGGAAAA AGCTGAAGTG TTACCTAATG 
CCGTTTGGAA CTGGAACCAG CAAATGTCCA GGCCGATTTT TTGCACTTAT GGAAATAAAA 
CAATTGTTGG TTATACTTTT AACTTATTTT GATTTAGAAA TAATTGATGA TAAGCCCATA 
GGACTAAACT ACAGCCGCTT GTTGTTTGGT ATTCAGTATC CAGATTCTGA TGTTTTATTT 
AGATACAAAG TGAAATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7B1 ( NM_004820.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004811.1 Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27221
Clone ID Related Accession (Same CDS sequence)	NM_004820.3 , NM_004820.4 , NM_004820.5
Accession Version	NM_004820.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-03
Organism	Homo sapiens(human)
Product	25-hydroxycholesterol 7-alpha-hydroxylase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104232.5, BC029155.1, BP349825.1, AC090136.6 and BQ575990.1. This sequence is a reference standard in the RefSeqGene project. On Jun 1, 2016 this sequence version replaced NM_004820.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.27723.1, SRR1660805.2650.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGGAGTTTA GATATGATCG TTTTATAGAA 
GATGGTAAGA AGAAAACCAC CTTTTTCAAA AGAGGGAAAA AGCTGAAGTG TTACCTAATG 
CCGTTTGGAA CTGGAACCAG CAAATGTCCA GGCCGATTTT TTGCACTTAT GGAAATAAAA 
CAATTGTTGG TTATACTTTT AACTTATTTT GATTTAGAAA TAATTGATGA TAAGCCCATA 
GGACTAAACT ACAGCCGCTT GTTGTTTGGT ATTCAGTATC CAGATTCTGA TGTTTTATTT 
AGATACAAAG TGAAATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004811.1
CDS	205..1725
Translation	MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIEDGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPIGLNYSRLLFGIQYPDSDVLFRYKVKS

Target ORF information:

RefSeq Version	NM_004820.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004820.4

ORF Insert Sequence:

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGGAGTTTA GATATGATCG TTTTATAGAA 
GATGGTAAGA AGAAAACCAC CTTTTTCAAA AGAGGGAAAA AGCTGAAGTG TTACCTAATG 
CCGTTTGGAA CTGGAACCAG CAAATGTCCA GGCCGATTTT TTGCACTTAT GGAAATAAAA 
CAATTGTTGG TTATACTTTT AACTTATTTT GATTTAGAAA TAATTGATGA TAAGCCCATA 
GGACTAAACT ACAGCCGCTT GTTGTTTGGT ATTCAGTATC CAGATTCTGA TGTTTTATTT 
AGATACAAAG TGAAATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7B1 ( NM_004820.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004811.1 Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27221
Clone ID Related Accession (Same CDS sequence)	NM_004820.3 , NM_004820.4 , NM_004820.5
Accession Version	NM_004820.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1521bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	cytochrome P450 7B1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104232.5, BC029155.1, BP349825.1, AC090136.6 and BQ575990.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004820.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.34537.1, SRR1660807.27723.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000310193.4/ ENSP00000310721.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGGAGTTTA GATATGATCG TTTTATAGAA 
GATGGTAAGA AGAAAACCAC CTTTTTCAAA AGAGGGAAAA AGCTGAAGTG TTACCTAATG 
CCGTTTGGAA CTGGAACCAG CAAATGTCCA GGCCGATTTT TTGCACTTAT GGAAATAAAA 
CAATTGTTGG TTATACTTTT AACTTATTTT GATTTAGAAA TAATTGATGA TAAGCCCATA 
GGACTAAACT ACAGCCGCTT GTTGTTTGGT ATTCAGTATC CAGATTCTGA TGTTTTATTT 
AGATACAAAG TGAAATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004811.1
CDS	151..1671
Translation	MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIEDGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPIGLNYSRLLFGIQYPDSDVLFRYKVKS

Target ORF information:

RefSeq Version	NM_004820.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004820.5

ORF Insert Sequence:

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGGAGTTTA GATATGATCG TTTTATAGAA 
GATGGTAAGA AGAAAACCAC CTTTTTCAAA AGAGGGAAAA AGCTGAAGTG TTACCTAATG 
CCGTTTGGAA CTGGAACCAG CAAATGTCCA GGCCGATTTT TTGCACTTAT GGAAATAAAA 
CAATTGTTGG TTATACTTTT AACTTATTTT GATTTAGAAA TAATTGATGA TAAGCCCATA 
GGACTAAACT ACAGCCGCTT GTTGTTTGGT ATTCAGTATC CAGATTCTGA TGTTTTATTT 
AGATACAAAG TGAAATCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7B1 ( NM_001324112.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311041.1 Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 2, mRNA.

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CloneID	OHu101740
Clone ID Related Accession (Same CDS sequence)	NM_001324112.2 , NM_001324112.1
Accession Version	NM_001324112.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	cytochrome P450 7B1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104232.5, BC029155.1, BM729103.1 and AC090136.6. On Jun 1, 2019 this sequence version replaced NM_001324112.1. Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region and contains an alternate 3' terminal exon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88852.1, SRR1803617.38957.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGCAAACTG TGCTCACAGG AGAAACTAGA 
GGAATGGAAA TAACAGAGCT CTCAAATCTG TACTTCTGTA CTGCCTACAG TAGAGAAAAG 
TGGAGAAGAG AAAGAAGGAA AATTCACACA CACATTCTAG TATACTTGAT AATTTTGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311041.1
CDS	151..1533
Translation	MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEQTVLTGETRGMEITELSNLYFCTAYSREKWRRERRKIHTHILVYLIILQ

Target ORF information:

RefSeq Version	NM_001324112.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324112.2

ORF Insert Sequence:

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGCAAACTG TGCTCACAGG AGAAACTAGA 
GGAATGGAAA TAACAGAGCT CTCAAATCTG TACTTCTGTA CTGCCTACAG TAGAGAAAAG 
TGGAGAAGAG AAAGAAGGAA AATTCACACA CACATTCTAG TATACTTGAT AATTTTGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7B1 ( NM_001324112.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001311041.1 Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 2, mRNA.

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CloneID	OHu101740
Clone ID Related Accession (Same CDS sequence)	NM_001324112.2 , NM_001324112.1
Accession Version	NM_001324112.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	25-hydroxycholesterol 7-alpha-hydroxylase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104232.5, BC029155.1, BM729103.1 and AC090136.6. Transcript Variant: This variant (2) contains an alternate exon in the 3' coding region and contains an alternate 3' terminal exon compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88852.1, SRR1803615.139122.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 5' end.

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGCAAACTG TGCTCACAGG AGAAACTAGA 
GGAATGGAAA TAACAGAGCT CTCAAATCTG TACTTCTGTA CTGCCTACAG TAGAGAAAAG 
TGGAGAAGAG AAAGAAGGAA AATTCACACA CACATTCTAG TATACTTGAT AATTTTGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001311041.1
CDS	205..1587
Translation	MAGEVSAATGRFSLERLGLPGLALAAALLLLALCLLVRRTRRPGEPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEQTVLTGETRGMEITELSNLYFCTAYSREKWRRERRKIHTHILVYLIILQ

Target ORF information:

RefSeq Version	NM_001324112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324112.1

ORF Insert Sequence:

ATGGCAGGAG AAGTGTCCGC GGCCACGGGC CGCTTTTCGC TGGAGCGGTT GGGCCTCCCG 
GGCCTGGCCC TCGCCGCGGC CCTGCTGCTC CTGGCCCTCT GCTTGCTTGT CCGGCGCACC 
AGGAGACCCG GTGAGCCTCC ATTGATAAAA GGTTGGCTTC CTTATCTTGG AGTGGTCCTG 
AACTTACGAA AAGACCCCTT AAGGTTCATG AAAACACTTC AAAAGCAACA TGGTGACACT 
TTCACAGTTC TTCTTGGTGG AAAGTACATA ACATTTATCC TGGACCCCTT CCAGTACCAG 
CTAGTGATAA AAAATCATAA ACAATTAAGC TTTCGAGTAT TTTCTAATAA ATTATTAGAG 
AAAGCATTTA GCATCAGTCA GTTGCAAAAA AATCATGACA TGAATGATGA GCTTCACCTC 
TGCTATCAAT TTTTGCAAGG CAAATCTTTG GACATACTCT TGGAAAGCAT GATGCAGAAT 
CTAAAACAAG TTTTTGAACC CCAGCTGTTA AAAACCACAA GTTGGGACAC GGCAGAACTG 
TATCCATTCT GCAGCTCAAT AATATTTGAG ATCACATTTA CAACTATATA TGGAAAAGTT 
ATTGTTTGTG ACAACAACAA ATTTATTAGT GAGCTAAGAG ATGATTTTTT AAAATTTGAT 
GACAAGTTTG CATATTTAGT ATCCAACATA CCCATTGAGC TTCTAGGAAA TGTCAAGTCT 
ATTAGAGAGA AAATTATAAA ATGCTTCTCA TCAGAAAAGT TAGCCAAGAT GCAAGGATGG 
TCAGAAGTTT TTCAAAGCAG GCAAGATGTC CTGGAGAAAT ATTATGTGCA CGAGGACCTT 
GAAATAGGAG CACATCATTT AGGCTTTCTC TGGGCCTCTG TGGCAAACAC TATTCCAACT 
ATGTTCTGGG CAATGTATTA TCTTCTGCGG CACCCAGAAG CTATGGCAGC AGTGCGTGAC 
GAAATTGACC GTTTGCTGCA GTCAACAGGT CAAAAGAAAG GGTCTGGATT TCCCATCCAC 
CTCACCAGAG AACAATTGGA CAGCCTAATC TGCCTAGAAA GCAGCATTTT TGAAGCTTTA 
CGACTGTCCT CATATTCAAC CACCATTCGT TTTGTTGAGG AGGATTTGAC TCTCAGTTCA 
GAGACCGGGG ACTACTGTGT GCGAAAGGGA GACTTGGTAG CCATCTTTCC TCCAGTCCTA 
CATGGTGACC CTGAAATCTT TGAAGCTCCA GAGCAAACTG TGCTCACAGG AGAAACTAGA 
GGAATGGAAA TAACAGAGCT CTCAAATCTG TACTTCTGTA CTGCCTACAG TAGAGAAAAG 
TGGAGAAGAG AAAGAAGGAA AATTCACACA CACATTCTAG TATACTTGAT AATTTTGCAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7B1 ( XM_017014002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869491.1 Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant X1, mRNA.

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CloneID	OHu98308
Clone ID Related Accession (Same CDS sequence)	XM_017014002.1
Accession Version	XM_017014002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cytochrome P450 7B1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTAA CATTGATGTT ACGTTGGTCT TCCAGAGGCT GCTTCATGAG AATAGAATCA 
GAGCAATCGA CAGTGGATAG TATTGTGGCT CTTTGGGAGG ACATTCAGTT GATGCTGACT 
GACTGCAATC CTTTGAGACA AGATTTGGAT ATCTATACTC TGCAACTTCC AGAAAAAACA 
TGTTTGAGGA GACCCGGTGA GCCTCCATTG ATAAAAGGTT GGCTTCCTTA TCTTGGAGTG 
GTCCTGAACT TACGAAAAGA CCCCTTAAGG TTCATGAAAA CACTTCAAAA GCAACATGGT 
GACACTTTCA CAGTTCTTCT TGGTGGAAAG TACATAACAT TTATCCTGGA CCCCTTCCAG 
TACCAGCTAG TGATAAAAAA TCATAAACAA TTAAGCTTTC GAGTATTTTC TAATAAATTA 
TTAGAGAAAG CATTTAGCAT CAGTCAGTTG CAAAAAAATC ATGACATGAA TGATGAGCTT 
CACCTCTGCT ATCAATTTTT GCAAGGCAAA TCTTTGGACA TACTCTTGGA AAGCATGATG 
CAGAATCTAA AACAAGTTTT TGAACCCCAG CTGTTAAAAA CCACAAGTTG GGACACGGCA 
GAACTGTATC CATTCTGCAG CTCAATAATA TTTGAGATCA CATTTACAAC TATATATGGA 
AAAGTTATTG TTTGTGACAA CAACAAATTT ATTAGTGAGC TAAGAGATGA TTTTTTAAAA 
TTTGATGACA AGTTTGCATA TTTAGTATCC AACATACCCA TTGAGCTTCT AGGAAATGTC 
AAGTCTATTA GAGAGAAAAT TATAAAATGC TTCTCATCAG AAAAGTTAGC CAAGATGCAA 
GGATGGTCAG AAGTTTTTCA AAGCAGGCAA GATGTCCTGG AGAAATATTA TGTGCACGAG 
GACCTTGAAA TAGGAGCACA TCATTTAGGC TTTCTCTGGG CCTCTGTGGC AAACACTATT 
CCAACTATGT TCTGGGCAAT GTATTATCTT CTGCGGCACC CAGAAGCTAT GGCAGCAGTG 
CGTGACGAAA TTGACCGTTT GCTGCAGTCA ACAGGTCAAA AGAAAGGGTC TGGATTTCCC 
ATCCACCTCA CCAGAGAACA ATTGGACAGC CTAATCTGCC TAGAAAGCAG CATTTTTGAA 
GCTTTACGAC TGTCCTCATA TTCAACCACC ATTCGTTTTG TTGAGGAGGA TTTGACTCTC 
AGTTCAGAGA CCGGGGACTA CTGTGTGCGA AAGGGAGACT TGGTAGCCAT CTTTCCTCCA 
GTCCTACATG GTGACCCTGA AATCTTTGAA GCTCCAGAGG AGTTTAGATA TGATCGTTTT 
ATAGAAGATG GTAAGAAGAA AACCACCTTT TTCAAAAGAG GGAAAAAGCT GAAGTGTTAC 
CTAATGCCGT TTGGAACTGG AACCAGCAAA TGTCCAGGCC GATTTTTTGC ACTTATGGAA 
ATAAAACAAT TGTTGGTTAT ACTTTTAACT TATTTTGATT TAGAAATAAT TGATGATAAG 
CCCATAGGAC TAAACTACAG CCGCTTGTTG TTTGGTATTC AGTATCCAGA TTCTGATGTT 
TTATTTAGAT ACAAAGTGAA ATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869491.1
CDS	18537..20123
Translation	MEVTLMLRWSSRGCFMRIESEQSTVDSIVALWEDIQLMLTDCNPLRQDLDIYTLQLPEKTCLRRPGEPPLIKGWLPYLGVVLNLRKDPLRFMKTLQKQHGDTFTVLLGGKYITFILDPFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGDPEIFEAPEEFRYDRFIEDGKKKTTFFKRGKKLKCYLMPFGTGTSKCPGRFFALMEIKQLLVILLTYFDLEIIDDKPIGLNYSRLLFGIQYPDSDVLFRYKVKS

Target ORF information:

RefSeq Version	XM_017014002.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily B member 1 (CYP7B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017014002.1

ORF Insert Sequence:

ATGGAGGTAA CATTGATGTT ACGTTGGTCT TCCAGAGGCT GCTTCATGAG AATAGAATCA 
GAGCAATCGA CAGTGGATAG TATTGTGGCT CTTTGGGAGG ACATTCAGTT GATGCTGACT 
GACTGCAATC CTTTGAGACA AGATTTGGAT ATCTATACTC TGCAACTTCC AGAAAAAACA 
TGTTTGAGGA GACCCGGTGA GCCTCCATTG ATAAAAGGTT GGCTTCCTTA TCTTGGAGTG 
GTCCTGAACT TACGAAAAGA CCCCTTAAGG TTCATGAAAA CACTTCAAAA GCAACATGGT 
GACACTTTCA CAGTTCTTCT TGGTGGAAAG TACATAACAT TTATCCTGGA CCCCTTCCAG 
TACCAGCTAG TGATAAAAAA TCATAAACAA TTAAGCTTTC GAGTATTTTC TAATAAATTA 
TTAGAGAAAG CATTTAGCAT CAGTCAGTTG CAAAAAAATC ATGACATGAA TGATGAGCTT 
CACCTCTGCT ATCAATTTTT GCAAGGCAAA TCTTTGGACA TACTCTTGGA AAGCATGATG 
CAGAATCTAA AACAAGTTTT TGAACCCCAG CTGTTAAAAA CCACAAGTTG GGACACGGCA 
GAACTGTATC CATTCTGCAG CTCAATAATA TTTGAGATCA CATTTACAAC TATATATGGA 
AAAGTTATTG TTTGTGACAA CAACAAATTT ATTAGTGAGC TAAGAGATGA TTTTTTAAAA 
TTTGATGACA AGTTTGCATA TTTAGTATCC AACATACCCA TTGAGCTTCT AGGAAATGTC 
AAGTCTATTA GAGAGAAAAT TATAAAATGC TTCTCATCAG AAAAGTTAGC CAAGATGCAA 
GGATGGTCAG AAGTTTTTCA AAGCAGGCAA GATGTCCTGG AGAAATATTA TGTGCACGAG 
GACCTTGAAA TAGGAGCACA TCATTTAGGC TTTCTCTGGG CCTCTGTGGC AAACACTATT 
CCAACTATGT TCTGGGCAAT GTATTATCTT CTGCGGCACC CAGAAGCTAT GGCAGCAGTG 
CGTGACGAAA TTGACCGTTT GCTGCAGTCA ACAGGTCAAA AGAAAGGGTC TGGATTTCCC 
ATCCACCTCA CCAGAGAACA ATTGGACAGC CTAATCTGCC TAGAAAGCAG CATTTTTGAA 
GCTTTACGAC TGTCCTCATA TTCAACCACC ATTCGTTTTG TTGAGGAGGA TTTGACTCTC 
AGTTCAGAGA CCGGGGACTA CTGTGTGCGA AAGGGAGACT TGGTAGCCAT CTTTCCTCCA 
GTCCTACATG GTGACCCTGA AATCTTTGAA GCTCCAGAGG AGTTTAGATA TGATCGTTTT 
ATAGAAGATG GTAAGAAGAA AACCACCTTT TTCAAAAGAG GGAAAAAGCT GAAGTGTTAC 
CTAATGCCGT TTGGAACTGG AACCAGCAAA TGTCCAGGCC GATTTTTTGC ACTTATGGAA 
ATAAAACAAT TGTTGGTTAT ACTTTTAACT TATTTTGATT TAGAAATAAT TGATGATAAG 
CCCATAGGAC TAAACTACAG CCGCTTGTTG TTTGGTATTC AGTATCCAGA TTCTGATGTT 
TTATTTAGAT ACAAAGTGAA ATCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-17 induces epithelial-mesenchymal transition consistent with the cancer stem cell phenotype by regulating CYP7B1 expression in colon cancer.
International journal of molecular medicine38(2)499-506(2016 Aug)
Xi XP,Zhuang J,Teng MJ,Xia LJ,Yang MY,Liu QG,Chen JB

Hereditary spastic paraplegia in Greece: characterisation of a previously unexplored population using next-generation sequencing.
European journal of human genetics : EJHG24(6)857-63(2016 06)
Lynch DS,Koutsis G,Tucci A,Panas M,Baklou M,Breza M,Karadima G,Houlden H

Mutational analysis of the CYP7B1, PNPLA6 and C19orf12 genes in autosomal recessive hereditary spastic paraplegia.
Molecular and cellular probes30(1)53-5(2016 Feb)
Schubert SF,Hoffjan S,Dekomien G

Variants in the CYP7B1 gene region do not affect natural resistance to HIV-1 infection.
Retrovirology1280(2015 Sep)
Sironi M,Biasin M,Pontremoli C,Cagliani R,Saulle I,Trabattoni D,Vichi F,Lo Caputo S,Mazzotta F,Aguilar-Jimenez W,Rugeles MT,Cedeno S,Sanchez J,Brander C,Clerici M

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CYP7B1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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