DDX23 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX23
Entrez Gene ID	9416
Full Name	DEAD-box helicase 23
Synonyms	PRPF28,SNRNP100,U5-100K,U5-100KD,prp28
General protein information	Preferred Names DEAD-box helicase 23 Names probable ATP-dependent RNA helicase DDX23 100 kDa U5 snRNP-specific protein DEAD (Asp-Glu-Ala-Asp) box polypeptide 23 DEAD box protein 23 PRP28 homolog, yeast PRP28p homolog U5 snRNP 100 kD protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.12
Summary	This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a component of the U5 snRNP complex; it may facilitate conformational changes in the spliceosome during nuclear pre-mRNA splicing. An alternatively spliced transcript variant has been found for this gene, but its biological validity has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	612172

mRNA and Protein(s)

mRNA	Protein	Name
NM_004818.2	NP_004809.2	probable ATP-dependent RNA helicase DDX23
NM_004818.3	NP_004809.2	probable ATP-dependent RNA helicase DDX23

Pathways from BioSystems

Homologies

Homo sapiens (human)	DDX23	NP_004809.2
Gallus gallus (chicken)	DDX23	XP_003643496.2
Danio rerio (zebrafish)	ddx23	NP_956176.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007825	XP_317676.4
	DDX23	XP_001104339.1
Mus musculus (house mouse)	Ddx23	NP_001074450.1
Canis lupus familiaris (dog)	DDX23	XP_851051.1
Schizosaccharomyces pombe (fission yeast)	prp28	NP_587984.1
Magnaporthe oryzae (rice blast fungus)	MGG_01583	XP_003714561.1
Oryza sativa (rice)	Os03g0708600	NP_001051038.1
Rattus norvegicus (Norway rat)	Ddx23	NP_001100263.2
Drosophila melanogaster (fruit fly)	CG10333	NP_609888.2
Caenorhabditis elegans (roundworm)	ddx-23	NP_498260.2
Neurospora crassa	NCU02803	XP_964459.1
Arabidopsis thaliana (thale cress)	AT2G33730	NP_180929.1
Pan troglodytes (chimpanzee)	DDX23	XP_509035.2
Bos taurus (cattle)	DDX23	NP_001095672.1

Gene Ontology
Bibliography

Related articles in PubMed

DDX23-Linc00630-HDAC1 axis activates the Notch pathway to promote metastasis.
Mao G, Jin H, Wu L
Oncotarget8(24)38937-38949(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Transcription Dynamics Prevent RNA-Mediated Genomic Instability through SRPK2-Dependent DDX23 Phosphorylation.
Sridhara SC, Carvalho S, Grosso AR, Gallego-Paez LM, Carmo-Fonseca M, de Almeida SF
Cell reports18(2)334-343(2017 01)

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.
Boesler C, Rigo N, Anokhina MM, Tauchert MJ, Agafonov DE, Kastner B, Urlaub H, Ficner R, Will CL, L?hrmann R
Nature communications711997(2016 Jul)

Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics.
Nicolai S, Filippi S, Caputo M, Cipak L, Gregan J, Ammerer G, Frontini M, Willems D, Prantera G, Balajee AS, Proietti-De-Santis L
PloS one10(6)e0128558(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pausing of RNA polymerase II (RNA Pol II) initiates a signaling cascade whereby the serine/arginine protein kinase 2 (SRPK2) phosphorylates the DDX23 helicase, culminating in the suppression of R-loops.
Title: Transcription Dynamics Prevent RNA-Mediated Genomic Instability through SRPK2-Dependent DDX23 Phosphorylation.

SRPK2 knock down results in hypophosphorylation of the arginine-serine (RS) domain-containing human PRP28 protein (PRP28, also known as DDX23), and destabilizes PRP28 association with the tri-snRNP.
Title: Phosphorylation of human PRP28 by SRPK2 is required for integration of the U4/U6-U5 tri-snRNP into the spliceosome.

The following DDX23 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX23 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04632	NM_004818.3 Latest version!	Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu04632	NM_004818.2	Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX23 ( NM_004818.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004809.2 Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.

Price & Availability↑

CloneID	OHu04632
Clone ID Related Accession (Same CDS sequence)	NM_004818.2 , NM_004818.3
Accession Version	NM_004818.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-29
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX23
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD709611.1, AK128428.1, BC002366.2 and AF026402.1. On Jan 26, 2004 this sequence version replaced NM_004818.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF026402.1, SRR1660805.154689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGGAG AGCTGGCTGA CAAAAAGGAC CGTGATGCAT CACCTTCCAA GGAGGAAAGG 
AAGCGATCAC GGACTCCTGA CAGAGAGCGG GATAGAGACC GGGACCGGAA GTCTTCCCCA 
TCTAAAGATA GAAAGCGGCA TCGTTCAAGG GATAGACGTC GAGGAGGCAG CCGTTCTCGC 
TCTCGTTCCC GTTCCAAATC TGCAGAAAGA GAACGACGGC ACAAAGAACG AGAACGAGAT 
AAGGAGCGGG ATCGGAATAA GAAGGACCGA GATCGAGACA AGGATGGGCA CAGACGGGAC 
AAGGACCGTA AACGATCCAG CTTATCTCCT GGTCGAGGAA AAGACTTTAA ATCTCGGAAG 
GACAGAGACT CTAAGAAGGA TGAAGAGGAT GAACATGGTG ATAAGAAGCC TAAGGCCCAG 
CCATTATCCC TGGAGGAGCT TCTGGCCAAG AAAAAGGCTG AGGAAGAAGC TGAGGCTAAG 
CCCAAGTTCC TCTCTAAAGC AGAACGAGAG GCTGAAGCTC TAAAGCGACG GCAGCAGGAG 
GTGGAAGAGC GGCAGAGGAT GCTTGAAGAA GAGAGGAAGA AAAGGAAACA GTTCCAAGAC 
TTGGGCAGGA AGATGTTGGA AGATCCTCAG GAACGGGAAC GTCGGGAACG CAGGGAGAGG 
ATGGAACGGG AGACCAATGG AAATGAGGAT GAGGAAGGGC GGCAGAAGAT CCGGGAAGAG 
AAGGATAAGA GCAAGGAACT GCATGCCATT AAGGAGCGTT ACCTGGGTGG CATCAAAAAG 
CGGCGCCGAA CGAGACATCT CAATGACCGG AAATTTGTTT TTGAGTGGGA TGCATCTGAG 
GACACATCCA TTGACTACAA CCCCCTGTAC AAAGAACGGC ACCAGGTGCA GTTGTTAGGG 
CGAGGCTTCA TTGCAGGCAT TGACCTCAAG CAGCAGAAGC GAGAGCAGTC ACGTTTCTAT 
GGAGACCTAA TGGAGAAGAG GCGAACCCTG GAAGAAAAGG AGCAGGAGGA GGCAAGACTC 
CGCAAACTTC GTAAGAAGGA AGCCAAGCAG CGCTGGGATG ATCGTCATTG GTCTCAGAAA 
AAGTTAGATG AGATGACGGA CAGGGACTGG CGGATCTTCC GTGAGGACTA CAGCATCACC 
ACCAAAGGTG GCAAGATCCC CAATCCCATC CGATCCTGGA AAGACTCTTC TCTGCCCCCA 
CACATCTTGG AGGTCATTGA TAAGTGTGGC TACAAGGAAC CAACACCTAT ACAGCGTCAG 
GCAATTCCCA TTGGGCTACA GAATCGTGAC ATCATTGGTG TGGCTGAGAC TGGCAGTGGC 
AAGACAGCAG CCTTCCTCAT CCCTCTGCTG GTCTGGATCA CCACACTTCC CAAAATTGAC 
AGGATCGAAG AGTCAGACCA AGGCCCTTAT GCCATCATCC TGGCTCCCAC CCGTGAGTTG 
GCTCAACAGA TTGAGGAAGA GACCATCAAG TTTGGGAAAC CGCTAGGTAT CCGCACTGTG 
GCTGTCATTG GTGGCATCTC CAGAGAAGAC CAGGGCTTCA GGCTGCGCAT GGGTTGTGAG 
ATTGTGATTG CTACCCCTGG GCGTTTGATT GATGTGCTGG AGAACCGCTA CCTGGTGCTG 
AGCCGCTGTA CCTATGTGGT TCTGGATGAG GCAGATAGGA TGATTGACAT GGGCTTTGAG 
CCAGATGTCC AGAAGATCCT GGAGCACATG CCTGTCAGCA ACCAGAAGCC AGACACGGAT 
GAGGCTGAGG ACCCTGAGAA GATGCTGGCC AACTTTGAGT CGGGAAAACA TAAGTACCGC 
CAAACAGTCA TGTTCACGGC CACCATGCCC CCAGCGGTGG AGCGTCTGGC CAGGAGCTAT 
CTTCGGCGAC CTGCTGTGGT GTACATTGGC TCCGCAGGCA AGCCCCATGA GCGTGTGGAA 
CAGAAGGTCT TCCTCATGTC AGAGTCAGAA AAGAGGAAAA AGCTGCTGGC AATCTTGGAG 
CAAGGCTTTG ACCCACCCAT CATTATTTTT GTCAACCAGA AGAAGGGCTG CGACGTGTTG 
GCCAAATCCC TGGAGAAGAT GGGGTACAAT GCTTGCACAC TGCACGGTGG AAAAGGCCAG 
GAGCAGCGAG AGTTTGCGTT GTCCAACCTC AAGGCTGGGG CCAAGGATAT TTTGGTGGCT 
ACAGATGTGG CTGGTCGTGG TATTGACATC CAAGATGTGT CTATGGTTGT CAACTATGAT 
ATGGCCAAAA ATATTGAAGA TTACATCCAC CGCATTGGCC GCACGGGACG AGCAGGCAAG 
AGTGGGGTGG CCATCACCTT CCTCACAAAA GAGGACTCTG CTGTGTTCTA CGAGCTGAAG 
CAAGCTATCC TGGAAAGCCC AGTGTCTTCC TGTCCCCCCG AACTAGCCAA CCACCCAGAT 
GCCCAGCATA AGCCAGGCAC CATCCTCACC AAGAAGCGCC GGGAAGAGAC CATCTTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004809.2
CDS	92..2554
Translation	MAGELADKKDRDASPSKEERKRSRTPDRERDRDRDRKSSPSKDRKRHRSRDRRRGGSRSRSRSRSKSAERERRHKERERDKERDRNKKDRDRDKDGHRRDKDRKRSSLSPGRGKDFKSRKDRDSKKDEEDEHGDKKPKAQPLSLEELLAKKKAEEEAEAKPKFLSKAEREAEALKRRQQEVEERQRMLEEERKKRKQFQDLGRKMLEDPQERERRERRERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA

Target ORF information:

RefSeq Version	NM_004818.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004818.2

ORF Insert Sequence:

ATGGCAGGAG AGCTGGCTGA CAAAAAGGAC CGTGATGCAT CACCTTCCAA GGAGGAAAGG 
AAGCGATCAC GGACTCCTGA CAGAGAGCGG GATAGAGACC GGGACCGGAA GTCTTCCCCA 
TCTAAAGATA GAAAGCGGCA TCGTTCAAGG GATAGACGTC GAGGAGGCAG CCGTTCTCGC 
TCTCGTTCCC GTTCCAAATC TGCAGAAAGA GAACGACGGC ACAAAGAACG AGAACGAGAT 
AAGGAGCGGG ATCGGAATAA GAAGGACCGA GATCGAGACA AGGATGGGCA CAGACGGGAC 
AAGGACCGTA AACGATCCAG CTTATCTCCT GGTCGAGGAA AAGACTTTAA ATCTCGGAAG 
GACAGAGACT CTAAGAAGGA TGAAGAGGAT GAACATGGTG ATAAGAAGCC TAAGGCCCAG 
CCATTATCCC TGGAGGAGCT TCTGGCCAAG AAAAAGGCTG AGGAAGAAGC TGAGGCTAAG 
CCCAAGTTCC TCTCTAAAGC AGAACGAGAG GCTGAAGCTC TAAAGCGACG GCAGCAGGAG 
GTGGAAGAGC GGCAGAGGAT GCTTGAAGAA GAGAGGAAGA AAAGGAAACA GTTCCAAGAC 
TTGGGCAGGA AGATGTTGGA AGATCCTCAG GAACGGGAAC GTCGGGAACG CAGGGAGAGG 
ATGGAACGGG AGACCAATGG AAATGAGGAT GAGGAAGGGC GGCAGAAGAT CCGGGAAGAG 
AAGGATAAGA GCAAGGAACT GCATGCCATT AAGGAGCGTT ACCTGGGTGG CATCAAAAAG 
CGGCGCCGAA CGAGACATCT CAATGACCGG AAATTTGTTT TTGAGTGGGA TGCATCTGAG 
GACACATCCA TTGACTACAA CCCCCTGTAC AAAGAACGGC ACCAGGTGCA GTTGTTAGGG 
CGAGGCTTCA TTGCAGGCAT TGACCTCAAG CAGCAGAAGC GAGAGCAGTC ACGTTTCTAT 
GGAGACCTAA TGGAGAAGAG GCGAACCCTG GAAGAAAAGG AGCAGGAGGA GGCAAGACTC 
CGCAAACTTC GTAAGAAGGA AGCCAAGCAG CGCTGGGATG ATCGTCATTG GTCTCAGAAA 
AAGTTAGATG AGATGACGGA CAGGGACTGG CGGATCTTCC GTGAGGACTA CAGCATCACC 
ACCAAAGGTG GCAAGATCCC CAATCCCATC CGATCCTGGA AAGACTCTTC TCTGCCCCCA 
CACATCTTGG AGGTCATTGA TAAGTGTGGC TACAAGGAAC CAACACCTAT ACAGCGTCAG 
GCAATTCCCA TTGGGCTACA GAATCGTGAC ATCATTGGTG TGGCTGAGAC TGGCAGTGGC 
AAGACAGCAG CCTTCCTCAT CCCTCTGCTG GTCTGGATCA CCACACTTCC CAAAATTGAC 
AGGATCGAAG AGTCAGACCA AGGCCCTTAT GCCATCATCC TGGCTCCCAC CCGTGAGTTG 
GCTCAACAGA TTGAGGAAGA GACCATCAAG TTTGGGAAAC CGCTAGGTAT CCGCACTGTG 
GCTGTCATTG GTGGCATCTC CAGAGAAGAC CAGGGCTTCA GGCTGCGCAT GGGTTGTGAG 
ATTGTGATTG CTACCCCTGG GCGTTTGATT GATGTGCTGG AGAACCGCTA CCTGGTGCTG 
AGCCGCTGTA CCTATGTGGT TCTGGATGAG GCAGATAGGA TGATTGACAT GGGCTTTGAG 
CCAGATGTCC AGAAGATCCT GGAGCACATG CCTGTCAGCA ACCAGAAGCC AGACACGGAT 
GAGGCTGAGG ACCCTGAGAA GATGCTGGCC AACTTTGAGT CGGGAAAACA TAAGTACCGC 
CAAACAGTCA TGTTCACGGC CACCATGCCC CCAGCGGTGG AGCGTCTGGC CAGGAGCTAT 
CTTCGGCGAC CTGCTGTGGT GTACATTGGC TCCGCAGGCA AGCCCCATGA GCGTGTGGAA 
CAGAAGGTCT TCCTCATGTC AGAGTCAGAA AAGAGGAAAA AGCTGCTGGC AATCTTGGAG 
CAAGGCTTTG ACCCACCCAT CATTATTTTT GTCAACCAGA AGAAGGGCTG CGACGTGTTG 
GCCAAATCCC TGGAGAAGAT GGGGTACAAT GCTTGCACAC TGCACGGTGG AAAAGGCCAG 
GAGCAGCGAG AGTTTGCGTT GTCCAACCTC AAGGCTGGGG CCAAGGATAT TTTGGTGGCT 
ACAGATGTGG CTGGTCGTGG TATTGACATC CAAGATGTGT CTATGGTTGT CAACTATGAT 
ATGGCCAAAA ATATTGAAGA TTACATCCAC CGCATTGGCC GCACGGGACG AGCAGGCAAG 
AGTGGGGTGG CCATCACCTT CCTCACAAAA GAGGACTCTG CTGTGTTCTA CGAGCTGAAG 
CAAGCTATCC TGGAAAGCCC AGTGTCTTCC TGTCCCCCCG AACTAGCCAA CCACCCAGAT 
GCCCAGCATA AGCCAGGCAC CATCCTCACC AAGAAGCGCC GGGAAGAGAC CATCTTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX23 ( NM_004818.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004809.2 Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.

Price & Availability↑

CloneID	OHu04632
Clone ID Related Accession (Same CDS sequence)	NM_004818.2 , NM_004818.3
Accession Version	NM_004818.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	probable ATP-dependent RNA helicase DDX23
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK128428.1, BC002366.2 and AF026402.1. On Nov 22, 2018 this sequence version replaced NM_004818.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF026402.1, SRR1660805.154689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308025.8/ ENSP00000310723.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGGAG AGCTGGCTGA CAAAAAGGAC CGTGATGCAT CACCTTCCAA GGAGGAAAGG 
AAGCGATCAC GGACTCCTGA CAGAGAGCGG GATAGAGACC GGGACCGGAA GTCTTCCCCA 
TCTAAAGATA GAAAGCGGCA TCGTTCAAGG GATAGACGTC GAGGAGGCAG CCGTTCTCGC 
TCTCGTTCCC GTTCCAAATC TGCAGAAAGA GAACGACGGC ACAAAGAACG AGAACGAGAT 
AAGGAGCGGG ATCGGAATAA GAAGGACCGA GATCGAGACA AGGATGGGCA CAGACGGGAC 
AAGGACCGTA AACGATCCAG CTTATCTCCT GGTCGAGGAA AAGACTTTAA ATCTCGGAAG 
GACAGAGACT CTAAGAAGGA TGAAGAGGAT GAACATGGTG ATAAGAAGCC TAAGGCCCAG 
CCATTATCCC TGGAGGAGCT TCTGGCCAAG AAAAAGGCTG AGGAAGAAGC TGAGGCTAAG 
CCCAAGTTCC TCTCTAAAGC AGAACGAGAG GCTGAAGCTC TAAAGCGACG GCAGCAGGAG 
GTGGAAGAGC GGCAGAGGAT GCTTGAAGAA GAGAGGAAGA AAAGGAAACA GTTCCAAGAC 
TTGGGCAGGA AGATGTTGGA AGATCCTCAG GAACGGGAAC GTCGGGAACG CAGGGAGAGG 
ATGGAACGGG AGACCAATGG AAATGAGGAT GAGGAAGGGC GGCAGAAGAT CCGGGAAGAG 
AAGGATAAGA GCAAGGAACT GCATGCCATT AAGGAGCGTT ACCTGGGTGG CATCAAAAAG 
CGGCGCCGAA CGAGACATCT CAATGACCGG AAATTTGTTT TTGAGTGGGA TGCATCTGAG 
GACACATCCA TTGACTACAA CCCCCTGTAC AAAGAACGGC ACCAGGTGCA GTTGTTAGGG 
CGAGGCTTCA TTGCAGGCAT TGACCTCAAG CAGCAGAAGC GAGAGCAGTC ACGTTTCTAT 
GGAGACCTAA TGGAGAAGAG GCGAACCCTG GAAGAAAAGG AGCAGGAGGA GGCAAGACTC 
CGCAAACTTC GTAAGAAGGA AGCCAAGCAG CGCTGGGATG ATCGTCATTG GTCTCAGAAA 
AAGTTAGATG AGATGACGGA CAGGGACTGG CGGATCTTCC GTGAGGACTA CAGCATCACC 
ACCAAAGGTG GCAAGATCCC CAATCCCATC CGATCCTGGA AAGACTCTTC TCTGCCCCCA 
CACATCTTGG AGGTCATTGA TAAGTGTGGC TACAAGGAAC CAACACCTAT ACAGCGTCAG 
GCAATTCCCA TTGGGCTACA GAATCGTGAC ATCATTGGTG TGGCTGAGAC TGGCAGTGGC 
AAGACAGCAG CCTTCCTCAT CCCTCTGCTG GTCTGGATCA CCACACTTCC CAAAATTGAC 
AGGATCGAAG AGTCAGACCA AGGCCCTTAT GCCATCATCC TGGCTCCCAC CCGTGAGTTG 
GCTCAACAGA TTGAGGAAGA GACCATCAAG TTTGGGAAAC CGCTAGGTAT CCGCACTGTG 
GCTGTCATTG GTGGCATCTC CAGAGAAGAC CAGGGCTTCA GGCTGCGCAT GGGTTGTGAG 
ATTGTGATTG CTACCCCTGG GCGTTTGATT GATGTGCTGG AGAACCGCTA CCTGGTGCTG 
AGCCGCTGTA CCTATGTGGT TCTGGATGAG GCAGATAGGA TGATTGACAT GGGCTTTGAG 
CCAGATGTCC AGAAGATCCT GGAGCACATG CCTGTCAGCA ACCAGAAGCC AGACACGGAT 
GAGGCTGAGG ACCCTGAGAA GATGCTGGCC AACTTTGAGT CGGGAAAACA TAAGTACCGC 
CAAACAGTCA TGTTCACGGC CACCATGCCC CCAGCGGTGG AGCGTCTGGC CAGGAGCTAT 
CTTCGGCGAC CTGCTGTGGT GTACATTGGC TCCGCAGGCA AGCCCCATGA GCGTGTGGAA 
CAGAAGGTCT TCCTCATGTC AGAGTCAGAA AAGAGGAAAA AGCTGCTGGC AATCTTGGAG 
CAAGGCTTTG ACCCACCCAT CATTATTTTT GTCAACCAGA AGAAGGGCTG CGACGTGTTG 
GCCAAATCCC TGGAGAAGAT GGGGTACAAT GCTTGCACAC TGCACGGTGG AAAAGGCCAG 
GAGCAGCGAG AGTTTGCGTT GTCCAACCTC AAGGCTGGGG CCAAGGATAT TTTGGTGGCT 
ACAGATGTGG CTGGTCGTGG TATTGACATC CAAGATGTGT CTATGGTTGT CAACTATGAT 
ATGGCCAAAA ATATTGAAGA TTACATCCAC CGCATTGGCC GCACGGGACG AGCAGGCAAG 
AGTGGGGTGG CCATCACCTT CCTCACAAAA GAGGACTCTG CTGTGTTCTA CGAGCTGAAG 
CAAGCTATCC TGGAAAGCCC AGTGTCTTCC TGTCCCCCCG AACTAGCCAA CCACCCAGAT 
GCCCAGCATA AGCCAGGCAC CATCCTCACC AAGAAGCGCC GGGAAGAGAC CATCTTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004809.2
CDS	81..2543
Translation	MAGELADKKDRDASPSKEERKRSRTPDRERDRDRDRKSSPSKDRKRHRSRDRRRGGSRSRSRSRSKSAERERRHKERERDKERDRNKKDRDRDKDGHRRDKDRKRSSLSPGRGKDFKSRKDRDSKKDEEDEHGDKKPKAQPLSLEELLAKKKAEEEAEAKPKFLSKAEREAEALKRRQQEVEERQRMLEEERKKRKQFQDLGRKMLEDPQERERRERRERMERETNGNEDEEGRQKIREEKDKSKELHAIKERYLGGIKKRRRTRHLNDRKFVFEWDASEDTSIDYNPLYKERHQVQLLGRGFIAGIDLKQQKREQSRFYGDLMEKRRTLEEKEQEEARLRKLRKKEAKQRWDDRHWSQKKLDEMTDRDWRIFREDYSITTKGGKIPNPIRSWKDSSLPPHILEVIDKCGYKEPTPIQRQAIPIGLQNRDIIGVAETGSGKTAAFLIPLLVWITTLPKIDRIEESDQGPYAIILAPTRELAQQIEEETIKFGKPLGIRTVAVIGGISREDQGFRLRMGCEIVIATPGRLIDVLENRYLVLSRCTYVVLDEADRMIDMGFEPDVQKILEHMPVSNQKPDTDEAEDPEKMLANFESGKHKYRQTVMFTATMPPAVERLARSYLRRPAVVYIGSAGKPHERVEQKVFLMSESEKRKKLLAILEQGFDPPIIIFVNQKKGCDVLAKSLEKMGYNACTLHGGKGQEQREFALSNLKAGAKDILVATDVAGRGIDIQDVSMVVNYDMAKNIEDYIHRIGRTGRAGKSGVAITFLTKEDSAVFYELKQAILESPVSSCPPELANHPDAQHKPGTILTKKRREETIFA

Target ORF information:

RefSeq Version	NM_004818.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 23 (DDX23), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004818.3

ORF Insert Sequence:

ATGGCAGGAG AGCTGGCTGA CAAAAAGGAC CGTGATGCAT CACCTTCCAA GGAGGAAAGG 
AAGCGATCAC GGACTCCTGA CAGAGAGCGG GATAGAGACC GGGACCGGAA GTCTTCCCCA 
TCTAAAGATA GAAAGCGGCA TCGTTCAAGG GATAGACGTC GAGGAGGCAG CCGTTCTCGC 
TCTCGTTCCC GTTCCAAATC TGCAGAAAGA GAACGACGGC ACAAAGAACG AGAACGAGAT 
AAGGAGCGGG ATCGGAATAA GAAGGACCGA GATCGAGACA AGGATGGGCA CAGACGGGAC 
AAGGACCGTA AACGATCCAG CTTATCTCCT GGTCGAGGAA AAGACTTTAA ATCTCGGAAG 
GACAGAGACT CTAAGAAGGA TGAAGAGGAT GAACATGGTG ATAAGAAGCC TAAGGCCCAG 
CCATTATCCC TGGAGGAGCT TCTGGCCAAG AAAAAGGCTG AGGAAGAAGC TGAGGCTAAG 
CCCAAGTTCC TCTCTAAAGC AGAACGAGAG GCTGAAGCTC TAAAGCGACG GCAGCAGGAG 
GTGGAAGAGC GGCAGAGGAT GCTTGAAGAA GAGAGGAAGA AAAGGAAACA GTTCCAAGAC 
TTGGGCAGGA AGATGTTGGA AGATCCTCAG GAACGGGAAC GTCGGGAACG CAGGGAGAGG 
ATGGAACGGG AGACCAATGG AAATGAGGAT GAGGAAGGGC GGCAGAAGAT CCGGGAAGAG 
AAGGATAAGA GCAAGGAACT GCATGCCATT AAGGAGCGTT ACCTGGGTGG CATCAAAAAG 
CGGCGCCGAA CGAGACATCT CAATGACCGG AAATTTGTTT TTGAGTGGGA TGCATCTGAG 
GACACATCCA TTGACTACAA CCCCCTGTAC AAAGAACGGC ACCAGGTGCA GTTGTTAGGG 
CGAGGCTTCA TTGCAGGCAT TGACCTCAAG CAGCAGAAGC GAGAGCAGTC ACGTTTCTAT 
GGAGACCTAA TGGAGAAGAG GCGAACCCTG GAAGAAAAGG AGCAGGAGGA GGCAAGACTC 
CGCAAACTTC GTAAGAAGGA AGCCAAGCAG CGCTGGGATG ATCGTCATTG GTCTCAGAAA 
AAGTTAGATG AGATGACGGA CAGGGACTGG CGGATCTTCC GTGAGGACTA CAGCATCACC 
ACCAAAGGTG GCAAGATCCC CAATCCCATC CGATCCTGGA AAGACTCTTC TCTGCCCCCA 
CACATCTTGG AGGTCATTGA TAAGTGTGGC TACAAGGAAC CAACACCTAT ACAGCGTCAG 
GCAATTCCCA TTGGGCTACA GAATCGTGAC ATCATTGGTG TGGCTGAGAC TGGCAGTGGC 
AAGACAGCAG CCTTCCTCAT CCCTCTGCTG GTCTGGATCA CCACACTTCC CAAAATTGAC 
AGGATCGAAG AGTCAGACCA AGGCCCTTAT GCCATCATCC TGGCTCCCAC CCGTGAGTTG 
GCTCAACAGA TTGAGGAAGA GACCATCAAG TTTGGGAAAC CGCTAGGTAT CCGCACTGTG 
GCTGTCATTG GTGGCATCTC CAGAGAAGAC CAGGGCTTCA GGCTGCGCAT GGGTTGTGAG 
ATTGTGATTG CTACCCCTGG GCGTTTGATT GATGTGCTGG AGAACCGCTA CCTGGTGCTG 
AGCCGCTGTA CCTATGTGGT TCTGGATGAG GCAGATAGGA TGATTGACAT GGGCTTTGAG 
CCAGATGTCC AGAAGATCCT GGAGCACATG CCTGTCAGCA ACCAGAAGCC AGACACGGAT 
GAGGCTGAGG ACCCTGAGAA GATGCTGGCC AACTTTGAGT CGGGAAAACA TAAGTACCGC 
CAAACAGTCA TGTTCACGGC CACCATGCCC CCAGCGGTGG AGCGTCTGGC CAGGAGCTAT 
CTTCGGCGAC CTGCTGTGGT GTACATTGGC TCCGCAGGCA AGCCCCATGA GCGTGTGGAA 
CAGAAGGTCT TCCTCATGTC AGAGTCAGAA AAGAGGAAAA AGCTGCTGGC AATCTTGGAG 
CAAGGCTTTG ACCCACCCAT CATTATTTTT GTCAACCAGA AGAAGGGCTG CGACGTGTTG 
GCCAAATCCC TGGAGAAGAT GGGGTACAAT GCTTGCACAC TGCACGGTGG AAAAGGCCAG 
GAGCAGCGAG AGTTTGCGTT GTCCAACCTC AAGGCTGGGG CCAAGGATAT TTTGGTGGCT 
ACAGATGTGG CTGGTCGTGG TATTGACATC CAAGATGTGT CTATGGTTGT CAACTATGAT 
ATGGCCAAAA ATATTGAAGA TTACATCCAC CGCATTGGCC GCACGGGACG AGCAGGCAAG 
AGTGGGGTGG CCATCACCTT CCTCACAAAA GAGGACTCTG CTGTGTTCTA CGAGCTGAAG 
CAAGCTATCC TGGAAAGCCC AGTGTCTTCC TGTCCCCCCG AACTAGCCAA CCACCCAGAT 
GCCCAGCATA AGCCAGGCAC CATCCTCACC AAGAAGCGCC GGGAAGAGAC CATCTTTGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DDX23-Linc00630-HDAC1 axis activates the Notch pathway to promote metastasis.
Oncotarget8(24)38937-38949(2017 Jun)
Mao G,Jin H,Wu L

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Transcription Dynamics Prevent RNA-Mediated Genomic Instability through SRPK2-Dependent DDX23 Phosphorylation.
Cell reports18(2)334-343(2017 01)
Sridhara SC,Carvalho S,Grosso AR,Gallego-Paez LM,Carmo-Fonseca M,de Almeida SF

A spliceosome intermediate with loosely associated tri-snRNP accumulates in the absence of Prp28 ATPase activity.
Nature communications711997(2016 Jul)
Boesler C,Rigo N,Anokhina MM,Tauchert MJ,Agafonov DE,Kastner B,Urlaub H,Ficner R,Will CL,L?hrmann R

Identification of Novel Proteins Co-Purifying with Cockayne Syndrome Group B (CSB) Reveals Potential Roles for CSB in RNA Metabolism and Chromatin Dynamics.
PloS one10(6)e0128558(2015)
Nicolai S,Filippi S,Caputo M,Cipak L,Gregan J,Ammerer G,Frontini M,Willems D,Prantera G,Balajee AS,Proietti-De-Santis L

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DDX23 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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