TJP2 cDNA ORF clone, Homo sapiens(human)

The following TJP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22892 NM_004817.4
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu108524 NM_001369870.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu108525 NM_001369873.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu08071 NM_001170415.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu11483 NM_201629.3
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $734.30
$1049.00
OHu27167 NM_001170414.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu29179 NM_001170416.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu43598 NM_001369875.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu62810 NM_001369871.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu99018 NM_001369872.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu99019 NM_001369874.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu43598 XM_005252314.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu62809 XM_011519204.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu62810 XM_011519206.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu62810 XM_011519207.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu62810 XM_011519208.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu62810 XM_011519209.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu99017 XM_017015326.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu99018 XM_017015327.2
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu99019 XM_017015328.1
Latest version!
Homo sapiens tight junction protein 2 (TJP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
View Old Accession Versions >>
OHu29179 NM_001170416.1 Homo sapiens tight junction protein 2 (TJP2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
OHu99018 XM_017015327.1 Homo sapiens tight junction protein 2 (TJP2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $559.30
$799.00
OHu22892 NM_004817.3 Homo sapiens tight junction protein 2 (TJP2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22892
    Clone ID Related Accession (Same CDS sequence) NM_004817.4 , NM_004817.3
    Accession Version NM_004817.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-14
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090695.1, L27476.1, BC027592.1 and AL358113.21. This sequence is a reference standard in the RefSeqGene project. On May 11, 2018 this sequence version replaced NM_004817.3. Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027592.1, L27476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000377245.9/ ENSP00000366453.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    AGCATAAGGA AACCCAGCCC AGAGCCACGA GCTCAGATGA GGAGGGCTGC TAGCAGCGAT
    CAACTTAGGG ACAATAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGCCAAAACC
    CAGAACAAAG AAGAATCCTA TGACTTCTCC AAATCCTATG AATATAAGTC AAACCCCTCT
    GCCGTTGCTG GTAATGAAAC TCCTGGGGCA TCTACCAAAG GTTATCCTCC TCCTGTTGCA
    GCAAAACCTA CCTTTGGGCG GTCTATACTG AAGCCCTCCA CTCCCATCCC TCCTCAAGAG
    GGTGAGGAGG TGGGAGAGAG CAGTGAGGAG CAAGATAATG CTCCCAAATC AGTCCTGGGC
    AAAGTCAAAA TATTTGAGAA GATGGATCAC AAGGCCAGGT TACAGAGAAT GCAGGAGCTC
    CAGGAAGCAC AGAATGCAAG GATCGAAATT GCCCAGAAGC ATCCTGATAT CTATGCAGTT
    CCAATCAAAA CGCACAAGCC AGACCCTGGC ACGCCCCAGC ACACGAGTTC CAGACCCCCT
    GAGCCACAGA AAGCTCCTTC CAGACCTTAT CAGGATACCA GAGGAAGTTA TGGCAGTGAT
    GCCGAGGAGG AGGAGTACCG CCAGCAGCTG TCAGAACACT CCAAGCGCGG TTACTATGGC
    CAGTCTGCCC GATACCGGGA CACAGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004808.2
    CDS97..3669
    Translation

    Target ORF information:

    RefSeq Version NM_004817.4
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004817.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    AGCATAAGGA AACCCAGCCC AGAGCCACGA GCTCAGATGA GGAGGGCTGC TAGCAGCGAT
    CAACTTAGGG ACAATAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGCCAAAACC
    CAGAACAAAG AAGAATCCTA TGACTTCTCC AAATCCTATG AATATAAGTC AAACCCCTCT
    GCCGTTGCTG GTAATGAAAC TCCTGGGGCA TCTACCAAAG GTTATCCTCC TCCTGTTGCA
    GCAAAACCTA CCTTTGGGCG GTCTATACTG AAGCCCTCCA CTCCCATCCC TCCTCAAGAG
    GGTGAGGAGG TGGGAGAGAG CAGTGAGGAG CAAGATAATG CTCCCAAATC AGTCCTGGGC
    AAAGTCAAAA TATTTGAGAA GATGGATCAC AAGGCCAGGT TACAGAGAAT GCAGGAGCTC
    CAGGAAGCAC AGAATGCAAG GATCGAAATT GCCCAGAAGC ATCCTGATAT CTATGCAGTT
    CCAATCAAAA CGCACAAGCC AGACCCTGGC ACGCCCCAGC ACACGAGTTC CAGACCCCCT
    GAGCCACAGA AAGCTCCTTC CAGACCTTAT CAGGATACCA GAGGAAGTTA TGGCAGTGAT
    GCCGAGGAGG AGGAGTACCG CCAGCAGCTG TCAGAACACT CCAAGCGCGG TTACTATGGC
    CAGTCTGCCC GATACCGGGA CACAGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22892
    Clone ID Related Accession (Same CDS sequence) NM_004817.4 , NM_004817.3
    Accession Version NM_004817.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3573bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090695.1, L27476.1, BC027592.1 and AL358113.21. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2009 this sequence version replaced NM_004817.2. Transcript Variant: This variant (1) encodes isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC027592.1, SRR1660807.203271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    AGCATAAGGA AACCCAGCCC AGAGCCACGA GCTCAGATGA GGAGGGCTGC TAGCAGCGAT
    CAACTTAGGG ACAATAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGCCAAAACC
    CAGAACAAAG AAGAATCCTA TGACTTCTCC AAATCCTATG AATATAAGTC AAACCCCTCT
    GCCGTTGCTG GTAATGAAAC TCCTGGGGCA TCTACCAAAG GTTATCCTCC TCCTGTTGCA
    GCAAAACCTA CCTTTGGGCG GTCTATACTG AAGCCCTCCA CTCCCATCCC TCCTCAAGAG
    GGTGAGGAGG TGGGAGAGAG CAGTGAGGAG CAAGATAATG CTCCCAAATC AGTCCTGGGC
    AAAGTCAAAA TATTTGAGAA GATGGATCAC AAGGCCAGGT TACAGAGAAT GCAGGAGCTC
    CAGGAAGCAC AGAATGCAAG GATCGAAATT GCCCAGAAGC ATCCTGATAT CTATGCAGTT
    CCAATCAAAA CGCACAAGCC AGACCCTGGC ACGCCCCAGC ACACGAGTTC CAGACCCCCT
    GAGCCACAGA AAGCTCCTTC CAGACCTTAT CAGGATACCA GAGGAAGTTA TGGCAGTGAT
    GCCGAGGAGG AGGAGTACCG CCAGCAGCTG TCAGAACACT CCAAGCGCGG TTACTATGGC
    CAGTCTGCCC GATACCGGGA CACAGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_004808.2
    CDS319..3891
    Translation

    Target ORF information:

    RefSeq Version NM_004817.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_004817.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    AGCATAAGGA AACCCAGCCC AGAGCCACGA GCTCAGATGA GGAGGGCTGC TAGCAGCGAT
    CAACTTAGGG ACAATAGCCC GCCCCCAGCA TTCAAGCCAG AGCCGCCCAA GGCCAAAACC
    CAGAACAAAG AAGAATCCTA TGACTTCTCC AAATCCTATG AATATAAGTC AAACCCCTCT
    GCCGTTGCTG GTAATGAAAC TCCTGGGGCA TCTACCAAAG GTTATCCTCC TCCTGTTGCA
    GCAAAACCTA CCTTTGGGCG GTCTATACTG AAGCCCTCCA CTCCCATCCC TCCTCAAGAG
    GGTGAGGAGG TGGGAGAGAG CAGTGAGGAG CAAGATAATG CTCCCAAATC AGTCCTGGGC
    AAAGTCAAAA TATTTGAGAA GATGGATCAC AAGGCCAGGT TACAGAGAAT GCAGGAGCTC
    CAGGAAGCAC AGAATGCAAG GATCGAAATT GCCCAGAAGC ATCCTGATAT CTATGCAGTT
    CCAATCAAAA CGCACAAGCC AGACCCTGGC ACGCCCCAGC ACACGAGTTC CAGACCCCCT
    GAGCCACAGA AAGCTCCTTC CAGACCTTAT CAGGATACCA GAGGAAGTTA TGGCAGTGAT
    GCCGAGGAGG AGGAGTACCG CCAGCAGCTG TCAGAACACT CCAAGCGCGG TTACTATGGC
    CAGTCTGCCC GATACCGGGA CACAGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108524
    Clone ID Related Accession (Same CDS sequence) NM_001369870.1
    Accession Version NM_001369870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 6
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162730.26, AL590238.9 and AL358113.21. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1909915.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG AGAGGACACG
    CCGAGCAGAT TGTCCAGGAT GGGTGCGACA CCCACTCCCT TTAAGTCCAC AGGGGATATT
    GCAGGCACAG TTGTCCCAGA GACCAACAAG GAACCCAGAT ACCAAGAGGA CCCCCCAGCT
    CCTCAACCAA AAGCAGCCCC GAGAACTTTT CTTCGTCCTA GTCCTGAAGA TGAAGCAATA
    TATGGCCCTA ATACCAAAAT GGTAAGGTTC AAGAAGGGAG ACAGCGTGGG CCTCCGGTTG
    GCTGGTGGCA ATGATGTCGG GATATTTGTT GCTGGCATTC AAGAAGGGAC CTCGGCGGAG
    CAGGAGGGCC TTCAAGAAGG AGACCAGATT CTGAAGGTGA ACACACAGGA TTTCAGAGGA
    TTAGTGCGGG AGGATGCCGT TCTCTACCTG TTAGAAATCC CTAAAGGTGA AATGGTGACC
    ATTTTAGCTC AGAGCCGAGC CGATGTGTAT AGAGACATCC TGGCTTGTGG CAGAGGGGAT
    TCGTTTTTTA TAAGAAGCCA CTTTGAATGT GAGAAGGAAA CTCCACAGAG CCTGGCCTTC
    ACCAGAGGGG AGGTCTTCCG AGTGGTAGAC ACACTGTATG ACGGCAAGCT GGGCAACTGG
    CTGGCTGTGA GGATTGGGAA CGAGTTGGAG AAAGGCTTAA TCCCCAACAA GAGCAGAGCT
    GAACAAATGG CCAGTGTTCA AAATGCCCAG AGAGACAACG CTGGGGACCG GGCAGATTTC
    TGGAGAATGC GTGGCCAGAG GTCTGGGGTG AAGAAGAACC TGAGGAAAAG TCGGGAAGAC
    CTCACAGCTG TTGTGTCTGT CAGCACCAAG TTCCCAGCTT ATGAGAGGGT TTTGCTGCGA
    GAAGCTGGTT TCAAGAGACC TGTGGTCTTA TTCGGCCCCA TAGCTGATAT AGCAATGGAA
    AAATTGGCTA ATGAGTTACC TGACTGGTTT CAAACTGCTA AAACGGAACC AAAAGATGCA
    GGATCTGAGA AATCCACTGG AGTGGTCCGG TTAAATACCG TGAGGCAAAT TATTGAACAG
    GATAAGCATG CACTACTGGA TGTGACTCCG AAAGCTGTGG ACCTGTTGAA TTACACCCAG
    TGGTTCCCAA TTGTGATTTT TTTCAACCCA GACTCCAGAC AAGGTGTCAA AACCATGAGA
    CAAAGGTTAA ATCCAACGTC CAACAAAAGT TCTCGAAAGT TATTTGATCA AGCCAACAAG
    CTTAAAAAAA CGTGTGCACA CCTTTTTACA GCTACAATCA ACCTAAATTC AGCCAATGAT
    AGCTGGTTTG GCAGCTTAAA GGACACTATT CAGCATCAGC AAGGAGAAGC GGTTTGGGTC
    TCTGAAGGAA AGATGGAAGG GATGGATGAT GACCCCGAAG ACCGCATGTC CTACTTAACC
    GCCATGGGCG CGGACTATCT GAGTTGCGAC AGCCGCCTCA TCAGTGACTT TGAAGACACG
    GACGGTGAAG GAGGCGCCTA CACTGACAAT GAGCTGGATG AGCCAGCCGA GGAGCCGCTG
    GTGTCGTCCA TCACCCGCTC CTCGGAGCCG GTGCAGCACG AGGAGAGCAT AAGGAAACCC
    AGCCCAGAGC CACGAGCTCA GATGAGGAGG GCTGCTAGCA GCGATCAACT TAGGGACAAT
    AGCCCGCCCC CAGCATTCAA GCCAGAGCCG CCCAAGGCCA AAACCCAGAA CAAAGAAGAA
    TCCTATGACT TCTCCAAATC CTATGAATAT AAGTCAAACC CCTCTGCCGT TGCTGGTAAT
    GAAACTCCTG GGGCATCTAC CAAAGGTTAT CCTCCTCCTG TTGCAGCAAA ACCTACCTTT
    GGGCGGTCTA TACTGAAGCC CTCCACTCCC ATCCCTCCTC AAGAGGGTGA GGAGGTGGGA
    GAGAGCAGTG AGGAGCAAGA TAATGCTCCC AAATCAGTCC TGGGCAAAGT CAAAATATTT
    GAGAAGATGG ATCACAAGGC CAGGTTACAG AGAATGCAGG AGCTCCAGGA AGCACAGAAT
    GCAAGGATCG AAATTGCCCA GAAGCATCCT GATATCTATG CAGTTCCAAT CAAAACGCAC
    AAGCCAGACC CTGGCACGCC CCAGCACACG AGTTCCAGAC CCCCTGAGCC ACAGAAAGCT
    CCTTCCAGAC CTTATCAGGA TACCAGAGGA AGTTATGGCA GTGATGCCGA GGAGGAGGAG
    TACCGCCAGC AGCTGTCAGA ACACTCCAAG CGCGGTTACT ATGGCCAGTC TGCCCGATAC
    CGGGACACAG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356799.1
    CDS358..3855
    Translation

    Target ORF information:

    RefSeq Version NM_001369870.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG AGAGGACACG
    CCGAGCAGAT TGTCCAGGAT GGGTGCGACA CCCACTCCCT TTAAGTCCAC AGGGGATATT
    GCAGGCACAG TTGTCCCAGA GACCAACAAG GAACCCAGAT ACCAAGAGGA CCCCCCAGCT
    CCTCAACCAA AAGCAGCCCC GAGAACTTTT CTTCGTCCTA GTCCTGAAGA TGAAGCAATA
    TATGGCCCTA ATACCAAAAT GGTAAGGTTC AAGAAGGGAG ACAGCGTGGG CCTCCGGTTG
    GCTGGTGGCA ATGATGTCGG GATATTTGTT GCTGGCATTC AAGAAGGGAC CTCGGCGGAG
    CAGGAGGGCC TTCAAGAAGG AGACCAGATT CTGAAGGTGA ACACACAGGA TTTCAGAGGA
    TTAGTGCGGG AGGATGCCGT TCTCTACCTG TTAGAAATCC CTAAAGGTGA AATGGTGACC
    ATTTTAGCTC AGAGCCGAGC CGATGTGTAT AGAGACATCC TGGCTTGTGG CAGAGGGGAT
    TCGTTTTTTA TAAGAAGCCA CTTTGAATGT GAGAAGGAAA CTCCACAGAG CCTGGCCTTC
    ACCAGAGGGG AGGTCTTCCG AGTGGTAGAC ACACTGTATG ACGGCAAGCT GGGCAACTGG
    CTGGCTGTGA GGATTGGGAA CGAGTTGGAG AAAGGCTTAA TCCCCAACAA GAGCAGAGCT
    GAACAAATGG CCAGTGTTCA AAATGCCCAG AGAGACAACG CTGGGGACCG GGCAGATTTC
    TGGAGAATGC GTGGCCAGAG GTCTGGGGTG AAGAAGAACC TGAGGAAAAG TCGGGAAGAC
    CTCACAGCTG TTGTGTCTGT CAGCACCAAG TTCCCAGCTT ATGAGAGGGT TTTGCTGCGA
    GAAGCTGGTT TCAAGAGACC TGTGGTCTTA TTCGGCCCCA TAGCTGATAT AGCAATGGAA
    AAATTGGCTA ATGAGTTACC TGACTGGTTT CAAACTGCTA AAACGGAACC AAAAGATGCA
    GGATCTGAGA AATCCACTGG AGTGGTCCGG TTAAATACCG TGAGGCAAAT TATTGAACAG
    GATAAGCATG CACTACTGGA TGTGACTCCG AAAGCTGTGG ACCTGTTGAA TTACACCCAG
    TGGTTCCCAA TTGTGATTTT TTTCAACCCA GACTCCAGAC AAGGTGTCAA AACCATGAGA
    CAAAGGTTAA ATCCAACGTC CAACAAAAGT TCTCGAAAGT TATTTGATCA AGCCAACAAG
    CTTAAAAAAA CGTGTGCACA CCTTTTTACA GCTACAATCA ACCTAAATTC AGCCAATGAT
    AGCTGGTTTG GCAGCTTAAA GGACACTATT CAGCATCAGC AAGGAGAAGC GGTTTGGGTC
    TCTGAAGGAA AGATGGAAGG GATGGATGAT GACCCCGAAG ACCGCATGTC CTACTTAACC
    GCCATGGGCG CGGACTATCT GAGTTGCGAC AGCCGCCTCA TCAGTGACTT TGAAGACACG
    GACGGTGAAG GAGGCGCCTA CACTGACAAT GAGCTGGATG AGCCAGCCGA GGAGCCGCTG
    GTGTCGTCCA TCACCCGCTC CTCGGAGCCG GTGCAGCACG AGGAGAGCAT AAGGAAACCC
    AGCCCAGAGC CACGAGCTCA GATGAGGAGG GCTGCTAGCA GCGATCAACT TAGGGACAAT
    AGCCCGCCCC CAGCATTCAA GCCAGAGCCG CCCAAGGCCA AAACCCAGAA CAAAGAAGAA
    TCCTATGACT TCTCCAAATC CTATGAATAT AAGTCAAACC CCTCTGCCGT TGCTGGTAAT
    GAAACTCCTG GGGCATCTAC CAAAGGTTAT CCTCCTCCTG TTGCAGCAAA ACCTACCTTT
    GGGCGGTCTA TACTGAAGCC CTCCACTCCC ATCCCTCCTC AAGAGGGTGA GGAGGTGGGA
    GAGAGCAGTG AGGAGCAAGA TAATGCTCCC AAATCAGTCC TGGGCAAAGT CAAAATATTT
    GAGAAGATGG ATCACAAGGC CAGGTTACAG AGAATGCAGG AGCTCCAGGA AGCACAGAAT
    GCAAGGATCG AAATTGCCCA GAAGCATCCT GATATCTATG CAGTTCCAAT CAAAACGCAC
    AAGCCAGACC CTGGCACGCC CCAGCACACG AGTTCCAGAC CCCCTGAGCC ACAGAAAGCT
    CCTTCCAGAC CTTATCAGGA TACCAGAGGA AGTTATGGCA GTGATGCCGA GGAGGAGGAG
    TACCGCCAGC AGCTGTCAGA ACACTCCAAG CGCGGTTACT ATGGCCAGTC TGCCCGATAC
    CGGGACACAG AATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108525
    Clone ID Related Accession (Same CDS sequence) NM_001369873.1
    Accession Version NM_001369873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 9
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL358113.21. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.141272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGGCC AAAACCCAGA ACAAAGAAGA ATCCTATGAC
    TTCTCCAAAT CCTATGAATA TAAGTCAAAC CCCTCTGCCG TTGCTGGTAA TGAAACTCCT
    GGGGCATCTA CCAAAGGTTA TCCTCCTCCT GTTGCAGCAA AACCTACCTT TGGGCGGTCT
    ATACTGAAGC CCTCCACTCC CATCCCTCCT CAAGAGGGTG AGGAGGTGGG AGAGAGCAGT
    GAGGAGCAAG ATAATGCTCC CAAATCAGTC CTGGGCAAAG TCAAAATATT TGAGAAGATG
    GATCACAAGG CCAGGTTACA GAGAATGCAG GAGCTCCAGG AAGCACAGAA TGCAAGGATC
    GAAATTGCCC AGAAGCATCC TGATATCTAT GCAGTTCCAA TCAAAACGCA CAAGCCAGAC
    CCTGGCACGC CCCAGCACAC GAGTTCCAGA CCCCCTGAGC CACAGAAAGC TCCTTCCAGA
    CCTTATCAGG ATACCAGAGG AAGTTATGGC AGTGATGCCG AGGAGGAGGA GTACCGCCAG
    CAGCTGTCAG AACACTCCAA GCGCGGTTAC TATGGCCAGT CTGCCCGATA CCGGGACACA
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356802.1
    CDS97..3345
    Translation

    Target ORF information:

    RefSeq Version NM_001369873.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGGCC AAAACCCAGA ACAAAGAAGA ATCCTATGAC
    TTCTCCAAAT CCTATGAATA TAAGTCAAAC CCCTCTGCCG TTGCTGGTAA TGAAACTCCT
    GGGGCATCTA CCAAAGGTTA TCCTCCTCCT GTTGCAGCAA AACCTACCTT TGGGCGGTCT
    ATACTGAAGC CCTCCACTCC CATCCCTCCT CAAGAGGGTG AGGAGGTGGG AGAGAGCAGT
    GAGGAGCAAG ATAATGCTCC CAAATCAGTC CTGGGCAAAG TCAAAATATT TGAGAAGATG
    GATCACAAGG CCAGGTTACA GAGAATGCAG GAGCTCCAGG AAGCACAGAA TGCAAGGATC
    GAAATTGCCC AGAAGCATCC TGATATCTAT GCAGTTCCAA TCAAAACGCA CAAGCCAGAC
    CCTGGCACGC CCCAGCACAC GAGTTCCAGA CCCCCTGAGC CACAGAAAGC TCCTTCCAGA
    CCTTATCAGG ATACCAGAGG AAGTTATGGC AGTGATGCCG AGGAGGAGGA GTACCGCCAG
    CAGCTGTCAG AACACTCCAA GCGCGGTTAC TATGGCCAGT CTGCCCGATA CCGGGACACA
    GAATTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu08071
    Clone ID Related Accession (Same CDS sequence) NM_001170415.1
    Accession Version NM_001170415.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3474bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC325189.1, AL358113.21 and AK302483.1. Transcript Variant: This variant (4) has multiple differences, compared to variant 1. These differences include a distinct 5' UTR and translation initiation at a downstream start codon, compared to variant 1. The encoded isoform (4) is shorter and has a distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.274273.1, SRR1660805.229776.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGGCCAAAAC CCAGAACAAA GAAGAATCCT ATGACTTCTC CAAATCCTAT
    GAATATAAGT CAAACCCCTC TGCCGTTGCT GGTAATGAAA CTCCTGGGGC ATCTACCAAA
    GGTTATCCTC CTCCTGTTGC AGCAAAACCT ACCTTTGGGC GGTCTATACT GAAGCCCTCC
    ACTCCCATCC CTCCTCAAGA GGGTGAGGAG GTGGGAGAGA GCAGTGAGGA GCAAGATAAT
    GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA ATATTTGAGA AGATGGATCA CAAGGCCAGG
    TTACAGAGAA TGCAGGAGCT CCAGGAAGCA CAGAATGCAA GGATCGAAAT TGCCCAGAAG
    CATCCTGATA TCTATGCAGT TCCAATCAAA ACGCACAAGC CAGACCCTGG CACGCCCCAG
    CACACGAGTT CCAGACCCCC TGAGCCACAG AAAGCTCCTT CCAGACCTTA TCAGGATACC
    AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG GAGGAGTACC GCCAGCAGCT GTCAGAACAC
    TCCAAGCGCG GTTACTATGG CCAGTCTGCC CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163886.1
    CDS152..3625
    Translation

    Target ORF information:

    RefSeq Version NM_001170415.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170415.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGGCCAAAAC CCAGAACAAA GAAGAATCCT ATGACTTCTC CAAATCCTAT
    GAATATAAGT CAAACCCCTC TGCCGTTGCT GGTAATGAAA CTCCTGGGGC ATCTACCAAA
    GGTTATCCTC CTCCTGTTGC AGCAAAACCT ACCTTTGGGC GGTCTATACT GAAGCCCTCC
    ACTCCCATCC CTCCTCAAGA GGGTGAGGAG GTGGGAGAGA GCAGTGAGGA GCAAGATAAT
    GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA ATATTTGAGA AGATGGATCA CAAGGCCAGG
    TTACAGAGAA TGCAGGAGCT CCAGGAAGCA CAGAATGCAA GGATCGAAAT TGCCCAGAAG
    CATCCTGATA TCTATGCAGT TCCAATCAAA ACGCACAAGC CAGACCCTGG CACGCCCCAG
    CACACGAGTT CCAGACCCCC TGAGCCACAG AAAGCTCCTT CCAGACCTTA TCAGGATACC
    AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG GAGGAGTACC GCCAGCAGCT GTCAGAACAC
    TCCAAGCGCG GTTACTATGG CCAGTCTGCC CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11483
    Clone ID Related Accession (Same CDS sequence) NM_201629.3
    Accession Version NM_201629.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090695.1, AK295016.1, AL358113.21, AK304447.1 and AA724570.1. On Jan 14, 2011 this sequence version replaced NM_201629.2. Transcript Variant: This variant (2) lacks two in-frame exons in the 3' coding region, compared to variant (1). The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.193838.1, SRR1660803.26079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    ATCGAAATTG CCCAGAAGCA TCCTGATATC TATGCAGTTC CAATCAAAAC GCACAAGCCA
    GACCCTGGCA CGCCCCAGCA CACGAGTTCC AGACCCCCTG AGCCACAGAA AGCTCCTTCC
    AGACCTTATC AGGATACCAG AGGAAGTTAT GGCAGTGATG CCGAGGAGGA GGAGTACCGC
    CAGCAGCTGT CAGAACACTC CAAGCGCGGT TACTATGGCC AGTCTGCCCG ATACCGGGAC
    ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_963923.1
    CDS319..3450
    Translation

    Target ORF information:

    RefSeq Version NM_201629.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_201629.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    ATCGAAATTG CCCAGAAGCA TCCTGATATC TATGCAGTTC CAATCAAAAC GCACAAGCCA
    GACCCTGGCA CGCCCCAGCA CACGAGTTCC AGACCCCCTG AGCCACAGAA AGCTCCTTCC
    AGACCTTATC AGGATACCAG AGGAAGTTAT GGCAGTGATG CCGAGGAGGA GGAGTACCGC
    CAGCAGCTGT CAGAACACTC CAAGCGCGGT TACTATGGCC AGTCTGCCCG ATACCGGGAC
    ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27167
    Clone ID Related Accession (Same CDS sequence) NM_001170414.2
    Accession Version NM_001170414.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB196171.1, AK295016.1, AI738923.1, DA270962.1, CX865985.1 and AA724570.1. On Dec 1, 2011 this sequence version replaced NM_001170414.1. Transcript Variant: This variant (5) has multiple differences, compared to variant 1. These differences include a distinct 5' UTR and translation initiation at a downstream start codon, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295016.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147920, SAMEA2158569 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GATCGAAATT
    GCCCAGAAGC ATCCTGATAT CTATGCAGTT CCAATCAAAA CGCACAAGCC AGACCCTGGC
    ACGCCCCAGC ACACGAGTTC CAGACCCCCT GAGCCACAGA AAGCTCCTTC CAGACCTTAT
    CAGGATACCA GAGGAAGTTA TGGCAGTGAT GCCGAGGAGG AGGAGTACCG CCAGCAGCTG
    TCAGAACACT CCAAGCGCGG TTACTATGGC CAGTCTGCCC GATACCGGGA CACAGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163885.1
    CDS233..3295
    Translation

    Target ORF information:

    RefSeq Version NM_001170414.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170414.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GATCGAAATT
    GCCCAGAAGC ATCCTGATAT CTATGCAGTT CCAATCAAAA CGCACAAGCC AGACCCTGGC
    ACGCCCCAGC ACACGAGTTC CAGACCCCCT GAGCCACAGA AAGCTCCTTC CAGACCTTAT
    CAGGATACCA GAGGAAGTTA TGGCAGTGAT GCCGAGGAGG AGGAGTACCG CCAGCAGCTG
    TCAGAACACT CCAAGCGCGG TTACTATGGC CAGTCTGCCC GATACCGGGA CACAGAATTA
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29179
    Clone ID Related Accession (Same CDS sequence) NM_001170416.2 , NM_001170416.1
    Accession Version NM_001170416.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL358113.21. On Apr 23, 2019 this sequence version replaced NM_001170416.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGTGAGTCC CTCTGCAAGC TCTCCCCTTG TTTTCCCCAA CCTTTCTTCA
    TGGGAAGGGG AGGGAAGCAA AACCATTCTC ACAGCCCCAG GCATGGAAGA GCTGATATGG
    GAACAGTACA CTGTGACCCT ACAAAAGGAT TCCAAAAGAG GATTTGGAAT TGCAGTGTCC
    GGAGGCAGAG ACAACCCCCA CTTTGAAAAT GGAGAAACGT CAATTGTCAT TTCTGATGTG
    CTCCCGGGTG GGCCTGCTGA TGGGCTGCTC CAAGAAAATG ACAGAGTGGT CATGGTCAAT
    GGCACCCCCA TGGAGGATGT GCTTCATTCG TTTGCAGTTC AGCAGCTCAG AAAAAGTGGG
    AAGGTCGCTG CTATTGTGGT CAAGAGGCCC CGGAAGGTCC AGGTGGCCGC ACTTCAGGCC
    AGCCCTCCCC TGGATCAGGA TGACCGGGCT TTTGAGGTGA TGGACGAGTT TGATGGCAGA
    AGTTTCCGGA GTGGCTACAG CGAGAGGAGC CGGCTGAACA GCCATGGGGG GCGCAGCCGC
    AGCTGGGAGG ACAGCCCGGA AAGGGGGCGT CCCCATGAGC GGGCCCGGAG CCGGGAGCGG
    GACCTCAGCC GGGACCGGAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCAAGACCAT
    GCGCGCACCC GAGACCGCAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCACGACTTT
    GGGCCATCCC GGGACCGGGA CCGTGACCGC AGCCGCGGCC GGAGCATTGA CCAGGACTAC
    GAGCGAGCCT ATCACCGGGC CTACGACCCA GACTACGAGC GGGCCTACAG CCCGGAGTAC
    AGGCGCGGGG CCCGCCACGA TGCCCGCTCT CGGGGACCCC GAAGCCGCAG CCGCGAGCAC
    CCGCACTCAC GGAGCCCCAG CCCCGAGCCT AGGGGGCGGC CGGGGCCCAT CGGGGTCCTC
    CTGATGAAAA GCAGAGCGAA CGAAGAGTAT GGTCTCCGGC TTGGGAGTCA GATCTTCGTA
    AAGGAAATGA CCCGAACGGG TCTGGCAACT AAAGATGGCA ACCTTCACGA AGGAGACATA
    ATTCTCAAGA TCAATGGGAC TGTAACTGAG AACATGTCTT TAACGGATGC TCGAAAATTG
    ATAGAAAAGT CAAGAGGAAA ACTACAGCTA GTGGTGTTGA GAGACAGCCA GCAGACCCTC
    ATCAACATCC CGTCATTAAA TGACAGTGAC TCAGAAATAG AAGATATTTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGTCATCAGT ATTCTGATTA TGATTATCAT
    TCCTCAAGTG AGAAGCTGAA GGAAAGGCCA AGTTCCAGAG AGGACACGCC GAGCAGATTG
    TCCAGGATGG GTGCGACACC CACTCCCTTT AAGTCCACAG GGGATATTGC AGGCACAGTT
    GTCCCAGAGA CCAACAAGGA ACCCAGATAC CAAGAGGACC CCCCAGCTCC TCAACCAAAA
    GCAGCCCCGA GAACTTTTCT TCGTCCTAGT CCTGAAGATG AAGCAATATA TGGCCCTAAT
    ACCAAAATGG TAAGGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGCAAT
    GATGTCGGGA TATTTGTTGC TGGCATTCAA GAAGGGACCT CGGCGGAGCA GGAGGGCCTT
    CAAGAAGGAG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGATT AGTGCGGGAG
    GATGCCGTTC TCTACCTGTT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCCG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGATTC GTTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAC CAGAGGGGAG
    GTCTTCCGAG TGGTAGACAC ACTGTATGAC GGCAAGCTGG GCAACTGGCT GGCTGTGAGG
    ATTGGGAACG AGTTGGAGAA AGGCTTAATC CCCAACAAGA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAAA ATGCCCAGAG AGACAACGCT GGGGACCGGG CAGATTTCTG GAGAATGCGT
    GGCCAGAGGT CTGGGGTGAA GAAGAACCTG AGGAAAAGTC GGGAAGACCT CACAGCTGTT
    GTGTCTGTCA GCACCAAGTT CCCAGCTTAT GAGAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT CGGCCCCATA GCTGATATAG CAATGGAAAA ATTGGCTAAT
    GAGTTACCTG ACTGGTTTCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCTGAGAAA
    TCCACTGGAG TGGTCCGGTT AAATACCGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTACTGGATG TGACTCCGAA AGCTGTGGAC CTGTTGAATT ACACCCAGTG GTTCCCAATT
    GTGATTTTTT TCAACCCAGA CTCCAGACAA GGTGTCAAAA CCATGAGACA AAGGTTAAAT
    CCAACGTCCA ACAAAAGTTC TCGAAAGTTA TTTGATCAAG CCAACAAGCT TAAAAAAACG
    TGTGCACACC TTTTTACAGC TACAATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTAAAGG ACACTATTCA GCATCAGCAA GGAGAAGCGG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCCGAAGAC CGCATGTCCT ACTTAACCGC CATGGGCGCG
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACGGA CGGTGAAGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCCGAGG AGCCGCTGGT GTCGTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCACGAG GAGAGCATAA GGAAACCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGC GATCAACTTA GGGACAATAG CCCGCCCCCA
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACCCAGAACA AAGAAGAATC CTATGACTTC
    TCCAAATCCT ATGAATATAA GTCAAACCCC TCTGCCGTTG CTGGTAATGA AACTCCTGGG
    GCATCTACCA AAGGTTATCC TCCTCCTGTT GCAGCAAAAC CTACCTTTGG GCGGTCTATA
    CTGAAGCCCT CCACTCCCAT CCCTCCTCAA GAGGGTGAGG AGGTGGGAGA GAGCAGTGAG
    GAGCAAGATA ATGCTCCCAA ATCAGTCCTG GGCAAAGTCA AAATATTTGA GAAGATGGAT
    CACAAGGCCA GGTTACAGAG AATGCAGGAG CTCCAGGAAG CACAGAATGC AAGGATCGAA
    ATTGCCCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACGCACAA GCCAGACCCT
    GGCACGCCCC AGCACACGAG TTCCAGACCC CCTGAGCCAC AGAAAGCTCC TTCCAGACCT
    TATCAGGATA CCAGAGGAAG TTATGGCAGT GATGCCGAGG AGGAGGAGTA CCGCCAGCAG
    CTGTCAGAAC ACTCCAAGCG CGGTTACTAT GGCCAGTCTG CCCGATACCG GGACACAGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163887.1
    CDS8..3673
    Translation

    Target ORF information:

    RefSeq Version NM_001170416.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170416.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGTGAGTCC CTCTGCAAGC TCTCCCCTTG TTTTCCCCAA CCTTTCTTCA
    TGGGAAGGGG AGGGAAGCAA AACCATTCTC ACAGCCCCAG GCATGGAAGA GCTGATATGG
    GAACAGTACA CTGTGACCCT ACAAAAGGAT TCCAAAAGAG GATTTGGAAT TGCAGTGTCC
    GGAGGCAGAG ACAACCCCCA CTTTGAAAAT GGAGAAACGT CAATTGTCAT TTCTGATGTG
    CTCCCGGGTG GGCCTGCTGA TGGGCTGCTC CAAGAAAATG ACAGAGTGGT CATGGTCAAT
    GGCACCCCCA TGGAGGATGT GCTTCATTCG TTTGCAGTTC AGCAGCTCAG AAAAAGTGGG
    AAGGTCGCTG CTATTGTGGT CAAGAGGCCC CGGAAGGTCC AGGTGGCCGC ACTTCAGGCC
    AGCCCTCCCC TGGATCAGGA TGACCGGGCT TTTGAGGTGA TGGACGAGTT TGATGGCAGA
    AGTTTCCGGA GTGGCTACAG CGAGAGGAGC CGGCTGAACA GCCATGGGGG GCGCAGCCGC
    AGCTGGGAGG ACAGCCCGGA AAGGGGGCGT CCCCATGAGC GGGCCCGGAG CCGGGAGCGG
    GACCTCAGCC GGGACCGGAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCAAGACCAT
    GCGCGCACCC GAGACCGCAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCACGACTTT
    GGGCCATCCC GGGACCGGGA CCGTGACCGC AGCCGCGGCC GGAGCATTGA CCAGGACTAC
    GAGCGAGCCT ATCACCGGGC CTACGACCCA GACTACGAGC GGGCCTACAG CCCGGAGTAC
    AGGCGCGGGG CCCGCCACGA TGCCCGCTCT CGGGGACCCC GAAGCCGCAG CCGCGAGCAC
    CCGCACTCAC GGAGCCCCAG CCCCGAGCCT AGGGGGCGGC CGGGGCCCAT CGGGGTCCTC
    CTGATGAAAA GCAGAGCGAA CGAAGAGTAT GGTCTCCGGC TTGGGAGTCA GATCTTCGTA
    AAGGAAATGA CCCGAACGGG TCTGGCAACT AAAGATGGCA ACCTTCACGA AGGAGACATA
    ATTCTCAAGA TCAATGGGAC TGTAACTGAG AACATGTCTT TAACGGATGC TCGAAAATTG
    ATAGAAAAGT CAAGAGGAAA ACTACAGCTA GTGGTGTTGA GAGACAGCCA GCAGACCCTC
    ATCAACATCC CGTCATTAAA TGACAGTGAC TCAGAAATAG AAGATATTTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGTCATCAGT ATTCTGATTA TGATTATCAT
    TCCTCAAGTG AGAAGCTGAA GGAAAGGCCA AGTTCCAGAG AGGACACGCC GAGCAGATTG
    TCCAGGATGG GTGCGACACC CACTCCCTTT AAGTCCACAG GGGATATTGC AGGCACAGTT
    GTCCCAGAGA CCAACAAGGA ACCCAGATAC CAAGAGGACC CCCCAGCTCC TCAACCAAAA
    GCAGCCCCGA GAACTTTTCT TCGTCCTAGT CCTGAAGATG AAGCAATATA TGGCCCTAAT
    ACCAAAATGG TAAGGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGCAAT
    GATGTCGGGA TATTTGTTGC TGGCATTCAA GAAGGGACCT CGGCGGAGCA GGAGGGCCTT
    CAAGAAGGAG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGATT AGTGCGGGAG
    GATGCCGTTC TCTACCTGTT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCCG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGATTC GTTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAC CAGAGGGGAG
    GTCTTCCGAG TGGTAGACAC ACTGTATGAC GGCAAGCTGG GCAACTGGCT GGCTGTGAGG
    ATTGGGAACG AGTTGGAGAA AGGCTTAATC CCCAACAAGA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAAA ATGCCCAGAG AGACAACGCT GGGGACCGGG CAGATTTCTG GAGAATGCGT
    GGCCAGAGGT CTGGGGTGAA GAAGAACCTG AGGAAAAGTC GGGAAGACCT CACAGCTGTT
    GTGTCTGTCA GCACCAAGTT CCCAGCTTAT GAGAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT CGGCCCCATA GCTGATATAG CAATGGAAAA ATTGGCTAAT
    GAGTTACCTG ACTGGTTTCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCTGAGAAA
    TCCACTGGAG TGGTCCGGTT AAATACCGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTACTGGATG TGACTCCGAA AGCTGTGGAC CTGTTGAATT ACACCCAGTG GTTCCCAATT
    GTGATTTTTT TCAACCCAGA CTCCAGACAA GGTGTCAAAA CCATGAGACA AAGGTTAAAT
    CCAACGTCCA ACAAAAGTTC TCGAAAGTTA TTTGATCAAG CCAACAAGCT TAAAAAAACG
    TGTGCACACC TTTTTACAGC TACAATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTAAAGG ACACTATTCA GCATCAGCAA GGAGAAGCGG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCCGAAGAC CGCATGTCCT ACTTAACCGC CATGGGCGCG
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACGGA CGGTGAAGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCCGAGG AGCCGCTGGT GTCGTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCACGAG GAGAGCATAA GGAAACCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGC GATCAACTTA GGGACAATAG CCCGCCCCCA
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACCCAGAACA AAGAAGAATC CTATGACTTC
    TCCAAATCCT ATGAATATAA GTCAAACCCC TCTGCCGTTG CTGGTAATGA AACTCCTGGG
    GCATCTACCA AAGGTTATCC TCCTCCTGTT GCAGCAAAAC CTACCTTTGG GCGGTCTATA
    CTGAAGCCCT CCACTCCCAT CCCTCCTCAA GAGGGTGAGG AGGTGGGAGA GAGCAGTGAG
    GAGCAAGATA ATGCTCCCAA ATCAGTCCTG GGCAAAGTCA AAATATTTGA GAAGATGGAT
    CACAAGGCCA GGTTACAGAG AATGCAGGAG CTCCAGGAAG CACAGAATGC AAGGATCGAA
    ATTGCCCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACGCACAA GCCAGACCCT
    GGCACGCCCC AGCACACGAG TTCCAGACCC CCTGAGCCAC AGAAAGCTCC TTCCAGACCT
    TATCAGGATA CCAGAGGAAG TTATGGCAGT GATGCCGAGG AGGAGGAGTA CCGCCAGCAG
    CTGTCAGAAC ACTCCAAGCG CGGTTACTAT GGCCAGTCTG CCCGATACCG GGACACAGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu29179
    Clone ID Related Accession (Same CDS sequence) NM_001170416.2 , NM_001170416.1
    Accession Version NM_001170416.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-23
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK295034.1 and AL358113.21. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148093, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGTGAGTCC CTCTGCAAGC TCTCCCCTTG TTTTCCCCAA CCTTTCTTCA
    TGGGAAGGGG AGGGAAGCAA AACCATTCTC ACAGCCCCAG GCATGGAAGA GCTGATATGG
    GAACAGTACA CTGTGACCCT ACAAAAGGAT TCCAAAAGAG GATTTGGAAT TGCAGTGTCC
    GGAGGCAGAG ACAACCCCCA CTTTGAAAAT GGAGAAACGT CAATTGTCAT TTCTGATGTG
    CTCCCGGGTG GGCCTGCTGA TGGGCTGCTC CAAGAAAATG ACAGAGTGGT CATGGTCAAT
    GGCACCCCCA TGGAGGATGT GCTTCATTCG TTTGCAGTTC AGCAGCTCAG AAAAAGTGGG
    AAGGTCGCTG CTATTGTGGT CAAGAGGCCC CGGAAGGTCC AGGTGGCCGC ACTTCAGGCC
    AGCCCTCCCC TGGATCAGGA TGACCGGGCT TTTGAGGTGA TGGACGAGTT TGATGGCAGA
    AGTTTCCGGA GTGGCTACAG CGAGAGGAGC CGGCTGAACA GCCATGGGGG GCGCAGCCGC
    AGCTGGGAGG ACAGCCCGGA AAGGGGGCGT CCCCATGAGC GGGCCCGGAG CCGGGAGCGG
    GACCTCAGCC GGGACCGGAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCAAGACCAT
    GCGCGCACCC GAGACCGCAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCACGACTTT
    GGGCCATCCC GGGACCGGGA CCGTGACCGC AGCCGCGGCC GGAGCATTGA CCAGGACTAC
    GAGCGAGCCT ATCACCGGGC CTACGACCCA GACTACGAGC GGGCCTACAG CCCGGAGTAC
    AGGCGCGGGG CCCGCCACGA TGCCCGCTCT CGGGGACCCC GAAGCCGCAG CCGCGAGCAC
    CCGCACTCAC GGAGCCCCAG CCCCGAGCCT AGGGGGCGGC CGGGGCCCAT CGGGGTCCTC
    CTGATGAAAA GCAGAGCGAA CGAAGAGTAT GGTCTCCGGC TTGGGAGTCA GATCTTCGTA
    AAGGAAATGA CCCGAACGGG TCTGGCAACT AAAGATGGCA ACCTTCACGA AGGAGACATA
    ATTCTCAAGA TCAATGGGAC TGTAACTGAG AACATGTCTT TAACGGATGC TCGAAAATTG
    ATAGAAAAGT CAAGAGGAAA ACTACAGCTA GTGGTGTTGA GAGACAGCCA GCAGACCCTC
    ATCAACATCC CGTCATTAAA TGACAGTGAC TCAGAAATAG AAGATATTTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGTCATCAGT ATTCTGATTA TGATTATCAT
    TCCTCAAGTG AGAAGCTGAA GGAAAGGCCA AGTTCCAGAG AGGACACGCC GAGCAGATTG
    TCCAGGATGG GTGCGACACC CACTCCCTTT AAGTCCACAG GGGATATTGC AGGCACAGTT
    GTCCCAGAGA CCAACAAGGA ACCCAGATAC CAAGAGGACC CCCCAGCTCC TCAACCAAAA
    GCAGCCCCGA GAACTTTTCT TCGTCCTAGT CCTGAAGATG AAGCAATATA TGGCCCTAAT
    ACCAAAATGG TAAGGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGCAAT
    GATGTCGGGA TATTTGTTGC TGGCATTCAA GAAGGGACCT CGGCGGAGCA GGAGGGCCTT
    CAAGAAGGAG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGATT AGTGCGGGAG
    GATGCCGTTC TCTACCTGTT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCCG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGATTC GTTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAC CAGAGGGGAG
    GTCTTCCGAG TGGTAGACAC ACTGTATGAC GGCAAGCTGG GCAACTGGCT GGCTGTGAGG
    ATTGGGAACG AGTTGGAGAA AGGCTTAATC CCCAACAAGA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAAA ATGCCCAGAG AGACAACGCT GGGGACCGGG CAGATTTCTG GAGAATGCGT
    GGCCAGAGGT CTGGGGTGAA GAAGAACCTG AGGAAAAGTC GGGAAGACCT CACAGCTGTT
    GTGTCTGTCA GCACCAAGTT CCCAGCTTAT GAGAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT CGGCCCCATA GCTGATATAG CAATGGAAAA ATTGGCTAAT
    GAGTTACCTG ACTGGTTTCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCTGAGAAA
    TCCACTGGAG TGGTCCGGTT AAATACCGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTACTGGATG TGACTCCGAA AGCTGTGGAC CTGTTGAATT ACACCCAGTG GTTCCCAATT
    GTGATTTTTT TCAACCCAGA CTCCAGACAA GGTGTCAAAA CCATGAGACA AAGGTTAAAT
    CCAACGTCCA ACAAAAGTTC TCGAAAGTTA TTTGATCAAG CCAACAAGCT TAAAAAAACG
    TGTGCACACC TTTTTACAGC TACAATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTAAAGG ACACTATTCA GCATCAGCAA GGAGAAGCGG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCCGAAGAC CGCATGTCCT ACTTAACCGC CATGGGCGCG
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACGGA CGGTGAAGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCCGAGG AGCCGCTGGT GTCGTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCACGAG GAGAGCATAA GGAAACCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGC GATCAACTTA GGGACAATAG CCCGCCCCCA
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACCCAGAACA AAGAAGAATC CTATGACTTC
    TCCAAATCCT ATGAATATAA GTCAAACCCC TCTGCCGTTG CTGGTAATGA AACTCCTGGG
    GCATCTACCA AAGGTTATCC TCCTCCTGTT GCAGCAAAAC CTACCTTTGG GCGGTCTATA
    CTGAAGCCCT CCACTCCCAT CCCTCCTCAA GAGGGTGAGG AGGTGGGAGA GAGCAGTGAG
    GAGCAAGATA ATGCTCCCAA ATCAGTCCTG GGCAAAGTCA AAATATTTGA GAAGATGGAT
    CACAAGGCCA GGTTACAGAG AATGCAGGAG CTCCAGGAAG CACAGAATGC AAGGATCGAA
    ATTGCCCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACGCACAA GCCAGACCCT
    GGCACGCCCC AGCACACGAG TTCCAGACCC CCTGAGCCAC AGAAAGCTCC TTCCAGACCT
    TATCAGGATA CCAGAGGAAG TTATGGCAGT GATGCCGAGG AGGAGGAGTA CCGCCAGCAG
    CTGTCAGAAC ACTCCAAGCG CGGTTACTAT GGCCAGTCTG CCCGATACCG GGACACAGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001163887.1
    CDS1..3666
    Translation

    Target ORF information:

    RefSeq Version NM_001170416.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGTGAGTCC CTCTGCAAGC TCTCCCCTTG TTTTCCCCAA CCTTTCTTCA
    TGGGAAGGGG AGGGAAGCAA AACCATTCTC ACAGCCCCAG GCATGGAAGA GCTGATATGG
    GAACAGTACA CTGTGACCCT ACAAAAGGAT TCCAAAAGAG GATTTGGAAT TGCAGTGTCC
    GGAGGCAGAG ACAACCCCCA CTTTGAAAAT GGAGAAACGT CAATTGTCAT TTCTGATGTG
    CTCCCGGGTG GGCCTGCTGA TGGGCTGCTC CAAGAAAATG ACAGAGTGGT CATGGTCAAT
    GGCACCCCCA TGGAGGATGT GCTTCATTCG TTTGCAGTTC AGCAGCTCAG AAAAAGTGGG
    AAGGTCGCTG CTATTGTGGT CAAGAGGCCC CGGAAGGTCC AGGTGGCCGC ACTTCAGGCC
    AGCCCTCCCC TGGATCAGGA TGACCGGGCT TTTGAGGTGA TGGACGAGTT TGATGGCAGA
    AGTTTCCGGA GTGGCTACAG CGAGAGGAGC CGGCTGAACA GCCATGGGGG GCGCAGCCGC
    AGCTGGGAGG ACAGCCCGGA AAGGGGGCGT CCCCATGAGC GGGCCCGGAG CCGGGAGCGG
    GACCTCAGCC GGGACCGGAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCAAGACCAT
    GCGCGCACCC GAGACCGCAG CCGTGGCCGG AGCCTGGAGC GGGGCCTGGA CCACGACTTT
    GGGCCATCCC GGGACCGGGA CCGTGACCGC AGCCGCGGCC GGAGCATTGA CCAGGACTAC
    GAGCGAGCCT ATCACCGGGC CTACGACCCA GACTACGAGC GGGCCTACAG CCCGGAGTAC
    AGGCGCGGGG CCCGCCACGA TGCCCGCTCT CGGGGACCCC GAAGCCGCAG CCGCGAGCAC
    CCGCACTCAC GGAGCCCCAG CCCCGAGCCT AGGGGGCGGC CGGGGCCCAT CGGGGTCCTC
    CTGATGAAAA GCAGAGCGAA CGAAGAGTAT GGTCTCCGGC TTGGGAGTCA GATCTTCGTA
    AAGGAAATGA CCCGAACGGG TCTGGCAACT AAAGATGGCA ACCTTCACGA AGGAGACATA
    ATTCTCAAGA TCAATGGGAC TGTAACTGAG AACATGTCTT TAACGGATGC TCGAAAATTG
    ATAGAAAAGT CAAGAGGAAA ACTACAGCTA GTGGTGTTGA GAGACAGCCA GCAGACCCTC
    ATCAACATCC CGTCATTAAA TGACAGTGAC TCAGAAATAG AAGATATTTC AGAAATAGAG
    TCAAACCGAT CATTTTCTCC AGAGGAGAGA CGTCATCAGT ATTCTGATTA TGATTATCAT
    TCCTCAAGTG AGAAGCTGAA GGAAAGGCCA AGTTCCAGAG AGGACACGCC GAGCAGATTG
    TCCAGGATGG GTGCGACACC CACTCCCTTT AAGTCCACAG GGGATATTGC AGGCACAGTT
    GTCCCAGAGA CCAACAAGGA ACCCAGATAC CAAGAGGACC CCCCAGCTCC TCAACCAAAA
    GCAGCCCCGA GAACTTTTCT TCGTCCTAGT CCTGAAGATG AAGCAATATA TGGCCCTAAT
    ACCAAAATGG TAAGGTTCAA GAAGGGAGAC AGCGTGGGCC TCCGGTTGGC TGGTGGCAAT
    GATGTCGGGA TATTTGTTGC TGGCATTCAA GAAGGGACCT CGGCGGAGCA GGAGGGCCTT
    CAAGAAGGAG ACCAGATTCT GAAGGTGAAC ACACAGGATT TCAGAGGATT AGTGCGGGAG
    GATGCCGTTC TCTACCTGTT AGAAATCCCT AAAGGTGAAA TGGTGACCAT TTTAGCTCAG
    AGCCGAGCCG ATGTGTATAG AGACATCCTG GCTTGTGGCA GAGGGGATTC GTTTTTTATA
    AGAAGCCACT TTGAATGTGA GAAGGAAACT CCACAGAGCC TGGCCTTCAC CAGAGGGGAG
    GTCTTCCGAG TGGTAGACAC ACTGTATGAC GGCAAGCTGG GCAACTGGCT GGCTGTGAGG
    ATTGGGAACG AGTTGGAGAA AGGCTTAATC CCCAACAAGA GCAGAGCTGA ACAAATGGCC
    AGTGTTCAAA ATGCCCAGAG AGACAACGCT GGGGACCGGG CAGATTTCTG GAGAATGCGT
    GGCCAGAGGT CTGGGGTGAA GAAGAACCTG AGGAAAAGTC GGGAAGACCT CACAGCTGTT
    GTGTCTGTCA GCACCAAGTT CCCAGCTTAT GAGAGGGTTT TGCTGCGAGA AGCTGGTTTC
    AAGAGACCTG TGGTCTTATT CGGCCCCATA GCTGATATAG CAATGGAAAA ATTGGCTAAT
    GAGTTACCTG ACTGGTTTCA AACTGCTAAA ACGGAACCAA AAGATGCAGG ATCTGAGAAA
    TCCACTGGAG TGGTCCGGTT AAATACCGTG AGGCAAATTA TTGAACAGGA TAAGCATGCA
    CTACTGGATG TGACTCCGAA AGCTGTGGAC CTGTTGAATT ACACCCAGTG GTTCCCAATT
    GTGATTTTTT TCAACCCAGA CTCCAGACAA GGTGTCAAAA CCATGAGACA AAGGTTAAAT
    CCAACGTCCA ACAAAAGTTC TCGAAAGTTA TTTGATCAAG CCAACAAGCT TAAAAAAACG
    TGTGCACACC TTTTTACAGC TACAATCAAC CTAAATTCAG CCAATGATAG CTGGTTTGGC
    AGCTTAAAGG ACACTATTCA GCATCAGCAA GGAGAAGCGG TTTGGGTCTC TGAAGGAAAG
    ATGGAAGGGA TGGATGATGA CCCCGAAGAC CGCATGTCCT ACTTAACCGC CATGGGCGCG
    GACTATCTGA GTTGCGACAG CCGCCTCATC AGTGACTTTG AAGACACGGA CGGTGAAGGA
    GGCGCCTACA CTGACAATGA GCTGGATGAG CCAGCCGAGG AGCCGCTGGT GTCGTCCATC
    ACCCGCTCCT CGGAGCCGGT GCAGCACGAG GAGAGCATAA GGAAACCCAG CCCAGAGCCA
    CGAGCTCAGA TGAGGAGGGC TGCTAGCAGC GATCAACTTA GGGACAATAG CCCGCCCCCA
    GCATTCAAGC CAGAGCCGCC CAAGGCCAAA ACCCAGAACA AAGAAGAATC CTATGACTTC
    TCCAAATCCT ATGAATATAA GTCAAACCCC TCTGCCGTTG CTGGTAATGA AACTCCTGGG
    GCATCTACCA AAGGTTATCC TCCTCCTGTT GCAGCAAAAC CTACCTTTGG GCGGTCTATA
    CTGAAGCCCT CCACTCCCAT CCCTCCTCAA GAGGGTGAGG AGGTGGGAGA GAGCAGTGAG
    GAGCAAGATA ATGCTCCCAA ATCAGTCCTG GGCAAAGTCA AAATATTTGA GAAGATGGAT
    CACAAGGCCA GGTTACAGAG AATGCAGGAG CTCCAGGAAG CACAGAATGC AAGGATCGAA
    ATTGCCCAGA AGCATCCTGA TATCTATGCA GTTCCAATCA AAACGCACAA GCCAGACCCT
    GGCACGCCCC AGCACACGAG TTCCAGACCC CCTGAGCCAC AGAAAGCTCC TTCCAGACCT
    TATCAGGATA CCAGAGGAAG TTATGGCAGT GATGCCGAGG AGGAGGAGTA CCGCCAGCAG
    CTGTCAGAAC ACTCCAAGCG CGGTTACTAT GGCCAGTCTG CCCGATACCG GGACACAGAA
    TTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43598
    Clone ID Related Accession (Same CDS sequence) XM_005252314.2 , NM_001369875.1
    Accession Version NM_001369875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 11
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL358113.21. On Apr 23, 2019 this sequence version replaced XM_005252314.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.77695.1, SRR1803616.171493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGCG ATCAACTTAG GGACAATAGC CCGCCCCCAG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAGAACAA AGAAGAATCC TATGACTTCT CCAAATCCTA TGAATATAAG
    TCAAACCCCT CTGCCGTTGC TGGTAATGAA ACTCCTGGGG CATCTACCAA AGGTTATCCT
    CCTCCTGTTG CAGCAAAACC TACCTTTGGG CGGTCTATAC TGAAGCCCTC CACTCCCATC
    CCTCCTCAAG AGGGTGAGGA GGTGGGAGAG AGCAGTGAGG AGCAAGATAA TGCTCCCAAA
    TCAGTCCTGG GCAAAGTCAA AATATTTGAG AAGATGGATC ACAAGGCCAG GTTACAGAGA
    ATGCAGGAGC TCCAGGAAGC ACAGAATGCA AGGATCGAAA TTGCCCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACGCACAAG CCAGACCCTG GCACGCCCCA GCACACGAGT
    TCCAGACCCC CTGAGCCACA GAAAGCTCCT TCCAGACCTT ATCAGGATAC CAGAGGAAGT
    TATGGCAGTG ATGCCGAGGA GGAGGAGTAC CGCCAGCAGC TGTCAGAACA CTCCAAGCGC
    GGTTACTATG GCCAGTCTGC CCGATACCGG GACACAGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356804.1
    CDS8..3592
    Translation

    Target ORF information:

    RefSeq Version NM_001369875.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGCG ATCAACTTAG GGACAATAGC CCGCCCCCAG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAGAACAA AGAAGAATCC TATGACTTCT CCAAATCCTA TGAATATAAG
    TCAAACCCCT CTGCCGTTGC TGGTAATGAA ACTCCTGGGG CATCTACCAA AGGTTATCCT
    CCTCCTGTTG CAGCAAAACC TACCTTTGGG CGGTCTATAC TGAAGCCCTC CACTCCCATC
    CCTCCTCAAG AGGGTGAGGA GGTGGGAGAG AGCAGTGAGG AGCAAGATAA TGCTCCCAAA
    TCAGTCCTGG GCAAAGTCAA AATATTTGAG AAGATGGATC ACAAGGCCAG GTTACAGAGA
    ATGCAGGAGC TCCAGGAAGC ACAGAATGCA AGGATCGAAA TTGCCCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACGCACAAG CCAGACCCTG GCACGCCCCA GCACACGAGT
    TCCAGACCCC CTGAGCCACA GAAAGCTCCT TCCAGACCTT ATCAGGATAC CAGAGGAAGT
    TATGGCAGTG ATGCCGAGGA GGAGGAGTAC CGCCAGCAGC TGTCAGAACA CTCCAAGCGC
    GGTTACTATG GCCAGTCTGC CCGATACCGG GACACAGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62810
    Clone ID Related Accession (Same CDS sequence) XM_011519207.2 , NM_001369871.1 , XM_011519209.2 , XM_011519206.2 , XM_011519208.2
    Accession Version NM_001369871.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 7
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590238.9 and AL358113.21. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1949221.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968832, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356800.1
    CDS348..3851
    Translation

    Target ORF information:

    RefSeq Version NM_001369871.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369871.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99018
    Clone ID Related Accession (Same CDS sequence) XM_017015327.2 , NM_001369872.1 , XM_017015327.1
    Accession Version NM_001369872.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 8
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL358113.21. On Apr 23, 2019 this sequence version replaced XM_017015327.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.103352.1, SRR1803615.8317.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356801.1
    CDS97..3558
    Translation

    Target ORF information:

    RefSeq Version NM_001369872.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369872.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99019
    Clone ID Related Accession (Same CDS sequence) XM_017015328.1 , NM_001369874.1
    Accession Version NM_001369874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform 10
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL358113.21. On Apr 23, 2019 this sequence version replaced XM_017015328.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.181986.1, SRR1660805.195335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356803.1
    CDS8..3151
    Translation

    Target ORF information:

    RefSeq Version NM_001369874.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu43598
    Clone ID Related Accession (Same CDS sequence) XM_005252314.2 , NM_001369875.1
    Accession Version XM_005252314.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005252314.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGCG ATCAACTTAG GGACAATAGC CCGCCCCCAG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAGAACAA AGAAGAATCC TATGACTTCT CCAAATCCTA TGAATATAAG
    TCAAACCCCT CTGCCGTTGC TGGTAATGAA ACTCCTGGGG CATCTACCAA AGGTTATCCT
    CCTCCTGTTG CAGCAAAACC TACCTTTGGG CGGTCTATAC TGAAGCCCTC CACTCCCATC
    CCTCCTCAAG AGGGTGAGGA GGTGGGAGAG AGCAGTGAGG AGCAAGATAA TGCTCCCAAA
    TCAGTCCTGG GCAAAGTCAA AATATTTGAG AAGATGGATC ACAAGGCCAG GTTACAGAGA
    ATGCAGGAGC TCCAGGAAGC ACAGAATGCA AGGATCGAAA TTGCCCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACGCACAAG CCAGACCCTG GCACGCCCCA GCACACGAGT
    TCCAGACCCC CTGAGCCACA GAAAGCTCCT TCCAGACCTT ATCAGGATAC CAGAGGAAGT
    TATGGCAGTG ATGCCGAGGA GGAGGAGTAC CGCCAGCAGC TGTCAGAACA CTCCAAGCGC
    GGTTACTATG GCCAGTCTGC CCGATACCGG GACACAGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005252371.1
    CDS360..3944
    Translation

    Target ORF information:

    RefSeq Version XM_005252314.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005252314.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGAGCATAAG GAAACCCAGC CCAGAGCCAC GAGCTCAGAT GAGGAGGGCT
    GCTAGCAGCG ATCAACTTAG GGACAATAGC CCGCCCCCAG CATTCAAGCC AGAGCCGCCC
    AAGGCCAAAA CCCAGAACAA AGAAGAATCC TATGACTTCT CCAAATCCTA TGAATATAAG
    TCAAACCCCT CTGCCGTTGC TGGTAATGAA ACTCCTGGGG CATCTACCAA AGGTTATCCT
    CCTCCTGTTG CAGCAAAACC TACCTTTGGG CGGTCTATAC TGAAGCCCTC CACTCCCATC
    CCTCCTCAAG AGGGTGAGGA GGTGGGAGAG AGCAGTGAGG AGCAAGATAA TGCTCCCAAA
    TCAGTCCTGG GCAAAGTCAA AATATTTGAG AAGATGGATC ACAAGGCCAG GTTACAGAGA
    ATGCAGGAGC TCCAGGAAGC ACAGAATGCA AGGATCGAAA TTGCCCAGAA GCATCCTGAT
    ATCTATGCAG TTCCAATCAA AACGCACAAG CCAGACCCTG GCACGCCCCA GCACACGAGT
    TCCAGACCCC CTGAGCCACA GAAAGCTCCT TCCAGACCTT ATCAGGATAC CAGAGGAAGT
    TATGGCAGTG ATGCCGAGGA GGAGGAGTAC CGCCAGCAGC TGTCAGAACA CTCCAAGCGC
    GGTTACTATG GCCAGTCTGC CCGATACCGG GACACAGAAT TATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62809
    Clone ID Related Accession (Same CDS sequence) XM_011519204.1
    Accession Version XM_011519204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GGCCAAAACC
    CAGAACAAAG AAGAATCCTA TGACTTCTCC AAATCCTATG AATATAAGTC AAACCCCTCT
    GCCGTTGCTG GTAATGAAAC TCCTGGGGCA TCTACCAAAG GTTATCCTCC TCCTGTTGCA
    GCAAAACCTA CCTTTGGGCG GTCTATACTG AAGCCCTCCA CTCCCATCCC TCCTCAAGAG
    GGTGAGGAGG TGGGAGAGAG CAGTGAGGAG CAAGATAATG CTCCCAAATC AGTCCTGGGC
    AAAGTCAAAA TATTTGAGAA GATGGATCAC AAGGCCAGGT TACAGAGAAT GCAGGAGCTC
    CAGGAAGCAC AGAATGCAAG GATCGAAATT GCCCAGAAGC ATCCTGATAT CTATGCAGTT
    CCAATCAAAA CGCACAAGCC AGACCCTGGC ACGCCCCAGC ACACGAGTTC CAGACCCCCT
    GAGCCACAGA AAGCTCCTTC CAGACCTTAT CAGGATACCA GAGGAAGTTA TGGCAGTGAT
    GCCGAGGAGG AGGAGTACCG CCAGCAGCTG TCAGAACACT CCAAGCGCGG TTACTATGGC
    CAGTCTGCCC GATACCGGGA CACAGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517506.1
    CDS131..3523
    Translation

    Target ORF information:

    RefSeq Version XM_011519204.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011519204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GGCCAAAACC
    CAGAACAAAG AAGAATCCTA TGACTTCTCC AAATCCTATG AATATAAGTC AAACCCCTCT
    GCCGTTGCTG GTAATGAAAC TCCTGGGGCA TCTACCAAAG GTTATCCTCC TCCTGTTGCA
    GCAAAACCTA CCTTTGGGCG GTCTATACTG AAGCCCTCCA CTCCCATCCC TCCTCAAGAG
    GGTGAGGAGG TGGGAGAGAG CAGTGAGGAG CAAGATAATG CTCCCAAATC AGTCCTGGGC
    AAAGTCAAAA TATTTGAGAA GATGGATCAC AAGGCCAGGT TACAGAGAAT GCAGGAGCTC
    CAGGAAGCAC AGAATGCAAG GATCGAAATT GCCCAGAAGC ATCCTGATAT CTATGCAGTT
    CCAATCAAAA CGCACAAGCC AGACCCTGGC ACGCCCCAGC ACACGAGTTC CAGACCCCCT
    GAGCCACAGA AAGCTCCTTC CAGACCTTAT CAGGATACCA GAGGAAGTTA TGGCAGTGAT
    GCCGAGGAGG AGGAGTACCG CCAGCAGCTG TCAGAACACT CCAAGCGCGG TTACTATGGC
    CAGTCTGCCC GATACCGGGA CACAGAATTA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62810
    Clone ID Related Accession (Same CDS sequence) XM_011519207.2 , NM_001369871.1 , XM_011519209.2 , XM_011519206.2 , XM_011519208.2
    Accession Version XM_011519207.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519207.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517509.1
    CDS229..3732
    Translation

    Target ORF information:

    RefSeq Version XM_011519207.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011519207.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62810
    Clone ID Related Accession (Same CDS sequence) XM_011519207.2 , NM_001369871.1 , XM_011519209.2 , XM_011519206.2 , XM_011519208.2
    Accession Version XM_011519209.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517511.1
    CDS18..3521
    Translation

    Target ORF information:

    RefSeq Version XM_011519209.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011519209.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62810
    Clone ID Related Accession (Same CDS sequence) XM_011519207.2 , NM_001369871.1 , XM_011519209.2 , XM_011519206.2 , XM_011519208.2
    Accession Version XM_011519206.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517508.1
    CDS733..4236
    Translation

    Target ORF information:

    RefSeq Version XM_011519206.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011519206.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu62810
    Clone ID Related Accession (Same CDS sequence) XM_011519207.2 , NM_001369871.1 , XM_011519209.2 , XM_011519206.2 , XM_011519208.2
    Accession Version XM_011519208.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011517510.1
    CDS189..3692
    Translation

    Target ORF information:

    RefSeq Version XM_011519208.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011519208.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAAGAGC TGATATGGGA ACAGTACACT GTGACCCTAC AAAAGGATTC CAAAAGAGGA 
    TTTGGAATTG CAGTGTCCGG AGGCAGAGAC AACCCCCACT TTGAAAATGG AGAAACGTCA
    ATTGTCATTT CTGATGTGCT CCCGGGTGGG CCTGCTGATG GGCTGCTCCA AGAAAATGAC
    AGAGTGGTCA TGGTCAATGG CACCCCCATG GAGGATGTGC TTCATTCGTT TGCAGTTCAG
    CAGCTCAGAA AAAGTGGGAA GGTCGCTGCT ATTGTGGTCA AGAGGCCCCG GAAGGTCCAG
    GTGGCCGCAC TTCAGGCCAG CCCTCCCCTG GATCAGGATG ACCGGGCTTT TGAGGTGATG
    GACGAGTTTG ATGGCAGAAG TTTCCGGAGT GGCTACAGCG AGAGGAGCCG GCTGAACAGC
    CATGGGGGGC GCAGCCGCAG CTGGGAGGAC AGCCCGGAAA GGGGGCGTCC CCATGAGCGG
    GCCCGGAGCC GGGAGCGGGA CCTCAGCCGG GACCGGAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC AAGACCATGC GCGCACCCGA GACCGCAGCC GTGGCCGGAG CCTGGAGCGG
    GGCCTGGACC ACGACTTTGG GCCATCCCGG GACCGGGACC GTGACCGCAG CCGCGGCCGG
    AGCATTGACC AGGACTACGA GCGAGCCTAT CACCGGGCCT ACGACCCAGA CTACGAGCGG
    GCCTACAGCC CGGAGTACAG GCGCGGGGCC CGCCACGATG CCCGCTCTCG GGGACCCCGA
    AGCCGCAGCC GCGAGCACCC GCACTCACGG AGCCCCAGCC CCGAGCCTAG GGGGCGGCCG
    GGGCCCATCG GGGTCCTCCT GATGAAAAGC AGAGCGAACG AAGAGTATGG TCTCCGGCTT
    GGGAGTCAGA TCTTCGTAAA GGAAATGACC CGAACGGGTC TGGCAACTAA AGATGGCAAC
    CTTCACGAAG GAGACATAAT TCTCAAGATC AATGGGACTG TAACTGAGAA CATGTCTTTA
    ACGGATGCTC GAAAATTGAT AGAAAAGTCA AGAGGAAAAC TACAGCTAGT GGTGTTGAGA
    GACAGCCAGC AGACCCTCAT CAACATCCCG TCATTAAATG ACAGTGACTC AGAAATAGAA
    GATATTTCAG AAATAGAGTC AAACCGATCA TTTTCTCCAG AGGAGAGACG TCATCAGTAT
    TCTGATTATG ATTATCATTC CTCAAGTGAG AAGCTGAAGG AAAGGCCAAG TTCCAGAGAG
    GACACGCCGA GCAGATTGTC CAGGATGGGT GCGACACCCA CTCCCTTTAA GTCCACAGGG
    GATATTGCAG GCACAGTTGT CCCAGAGACC AACAAGGAAC CCAGATACCA AGAGGACCCC
    CCAGCTCCTC AACCAAAAGC AGCCCCGAGA ACTTTTCTTC GTCCTAGTCC TGAAGATGAA
    GCAATATATG GCCCTAATAC CAAAATGGTA AGGTTCAAGA AGGGAGACAG CGTGGGCCTC
    CGGTTGGCTG GTGGCAATGA TGTCGGGATA TTTGTTGCTG GCATTCAAGA AGGGACCTCG
    GCGGAGCAGG AGGGCCTTCA AGAAGGAGAC CAGATTCTGA AGGTGAACAC ACAGGATTTC
    AGAGGATTAG TGCGGGAGGA TGCCGTTCTC TACCTGTTAG AAATCCCTAA AGGTGAAATG
    GTGACCATTT TAGCTCAGAG CCGAGCCGAT GTGTATAGAG ACATCCTGGC TTGTGGCAGA
    GGGGATTCGT TTTTTATAAG AAGCCACTTT GAATGTGAGA AGGAAACTCC ACAGAGCCTG
    GCCTTCACCA GAGGGGAGGT CTTCCGAGTG GTAGACACAC TGTATGACGG CAAGCTGGGC
    AACTGGCTGG CTGTGAGGAT TGGGAACGAG TTGGAGAAAG GCTTAATCCC CAACAAGAGC
    AGAGCTGAAC AAATGGCCAG TGTTCAAAAT GCCCAGAGAG ACAACGCTGG GGACCGGGCA
    GATTTCTGGA GAATGCGTGG CCAGAGGTCT GGGGTGAAGA AGAACCTGAG GAAAAGTCGG
    GAAGACCTCA CAGCTGTTGT GTCTGTCAGC ACCAAGTTCC CAGCTTATGA GAGGGTTTTG
    CTGCGAGAAG CTGGTTTCAA GAGACCTGTG GTCTTATTCG GCCCCATAGC TGATATAGCA
    ATGGAAAAAT TGGCTAATGA GTTACCTGAC TGGTTTCAAA CTGCTAAAAC GGAACCAAAA
    GATGCAGGAT CTGAGAAATC CACTGGAGTG GTCCGGTTAA ATACCGTGAG GCAAATTATT
    GAACAGGATA AGCATGCACT ACTGGATGTG ACTCCGAAAG CTGTGGACCT GTTGAATTAC
    ACCCAGTGGT TCCCAATTGT GATTTTTTTC AACCCAGACT CCAGACAAGG TGTCAAAACC
    ATGAGACAAA GGTTAAATCC AACGTCCAAC AAAAGTTCTC GAAAGTTATT TGATCAAGCC
    AACAAGCTTA AAAAAACGTG TGCACACCTT TTTACAGCTA CAATCAACCT AAATTCAGCC
    AATGATAGCT GGTTTGGCAG CTTAAAGGAC ACTATTCAGC ATCAGCAAGG AGAAGCGGTT
    TGGGTCTCTG AAGGAAAGAT GGAAGGGATG GATGATGACC CCGAAGACCG CATGTCCTAC
    TTAACCGCCA TGGGCGCGGA CTATCTGAGT TGCGACAGCC GCCTCATCAG TGACTTTGAA
    GACACGGACG GTGAAGGAGG CGCCTACACT GACAATGAGC TGGATGAGCC AGCCGAGGAG
    CCGCTGGTGT CGTCCATCAC CCGCTCCTCG GAGCCGGTGC AGCACGAGGA GAGCATAAGG
    AAACCCAGCC CAGAGCCACG AGCTCAGATG AGGAGGGCTG CTAGCAGCGA TCAACTTAGG
    GACAATAGCC CGCCCCCAGC ATTCAAGCCA GAGCCGCCCA AGGCCAAAAC CCAGAACAAA
    GAAGAATCCT ATGACTTCTC CAAATCCTAT GAATATAAGT CAAACCCCTC TGCCGTTGCT
    GGTAATGAAA CTCCTGGGGC ATCTACCAAA GGTTATCCTC CTCCTGTTGC AGCAAAACCT
    ACCTTTGGGC GGTCTATACT GAAGCCCTCC ACTCCCATCC CTCCTCAAGA GGGTGAGGAG
    GTGGGAGAGA GCAGTGAGGA GCAAGATAAT GCTCCCAAAT CAGTCCTGGG CAAAGTCAAA
    ATATTTGAGA AGATGGATCA CAAGGCCAGG TTACAGAGAA TGCAGGAGCT CCAGGAAGCA
    CAGAATGCAA GGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99017
    Clone ID Related Accession (Same CDS sequence) XM_017015326.1
    Accession Version XM_017015326.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGGGG TGCGAAGTAC CACATTACTG TCATCTCCCT GTGAGTGGGA TTGGCACCCC 
    GGCTGCAGCT CCAGGAGCAA GTACTCCAGG AAGCACAGAG CTGTACCAGT ACACGGCCCA
    CGTTGCCAGG CCCCAGGCAT GGAAGAGCTG ATATGGGAAC AGTACACTGT GACCCTACAA
    AAGGATTCCA AAAGAGGATT TGGAATTGCA GTGTCCGGAG GCAGAGACAA CCCCCACTTT
    GAAAATGGAG AAACGTCAAT TGTCATTTCT GATGTGCTCC CGGGTGGGCC TGCTGATGGG
    CTGCTCCAAG AAAATGACAG AGTGGTCATG GTCAATGGCA CCCCCATGGA GGATGTGCTT
    CATTCGTTTG CAGTTCAGCA GCTCAGAAAA AGTGGGAAGG TCGCTGCTAT TGTGGTCAAG
    AGGCCCCGGA AGGTCCAGGT GGCCGCACTT CAGGCCAGCC CTCCCCTGGA TCAGGATGAC
    CGGGCTTTTG AGGTGATGGA CGAGTTTGAT GGCAGAAGTT TCCGGAGTGG CTACAGCGAG
    AGGAGCCGGC TGAACAGCCA TGGGGGGCGC AGCCGCAGCT GGGAGGACAG CCCGGAAAGG
    GGGCGTCCCC ATGAGCGGGC CCGGAGCCGG GAGCGGGACC TCAGCCGGGA CCGGAGCCGT
    GGCCGGAGCC TGGAGCGGGG CCTGGACCAA GACCATGCGC GCACCCGAGA CCGCAGCCGT
    GGCCGGAGCC TGGAGCGGGG CCTGGACCAC GACTTTGGGC CATCCCGGGA CCGGGACCGT
    GACCGCAGCC GCGGCCGGAG CATTGACCAG GACTACGAGC GAGCCTATCA CCGGGCCTAC
    GACCCAGACT ACGAGCGGGC CTACAGCCCG GAGTACAGGC GCGGGGCCCG CCACGATGCC
    CGCTCTCGGG GACCCCGAAG CCGCAGCCGC GAGCACCCGC ACTCACGGAG CCCCAGCCCC
    GAGCCTAGGG GGCGGCCGGG GCCCATCGGG GTCCTCCTGA TGAAAAGCAG AGCGAACGAA
    GAGTATGGTC TCCGGCTTGG GAGTCAGATC TTCGTAAAGG AAATGACCCG AACGGGTCTG
    GCAACTAAAG ATGGCAACCT TCACGAAGGA GACATAATTC TCAAGATCAA TGGGACTGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAATTGATAG AAAAGTCAAG AGGAAAACTA
    CAGCTAGTGG TGTTGAGAGA CAGCCAGCAG ACCCTCATCA ACATCCCGTC ATTAAATGAC
    AGTGACTCAG AAATAGAAGA TATTTCAGAA ATAGAGTCAA ACCGATCATT TTCTCCAGAG
    GAGAGACGTC ATCAGTATTC TGATTATGAT TATCATTCCT CAAGTGAGAA GCTGAAGGAA
    AGGCCAAGTT CCAGAGAGGA CACGCCGAGC AGATTGTCCA GGATGGGTGC GACACCCACT
    CCCTTTAAGT CCACAGGGGA TATTGCAGGC ACAGTTGTCC CAGAGACCAA CAAGGAACCC
    AGATACCAAG AGGACCCCCC AGCTCCTCAA CCAAAAGCAG CCCCGAGAAC TTTTCTTCGT
    CCTAGTCCTG AAGATGAAGC AATATATGGC CCTAATACCA AAATGGTAAG GTTCAAGAAG
    GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGCAATGATG TCGGGATATT TGTTGCTGGC
    ATTCAAGAAG GGACCTCGGC GGAGCAGGAG GGCCTTCAAG AAGGAGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGATTAGTG CGGGAGGATG CCGTTCTCTA CCTGTTAGAA
    ATCCCTAAAG GTGAAATGGT GACCATTTTA GCTCAGAGCC GAGCCGATGT GTATAGAGAC
    ATCCTGGCTT GTGGCAGAGG GGATTCGTTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGAGTGGT AGACACACTG
    TATGACGGCA AGCTGGGCAA CTGGCTGGCT GTGAGGATTG GGAACGAGTT GGAGAAAGGC
    TTAATCCCCA ACAAGAGCAG AGCTGAACAA ATGGCCAGTG TTCAAAATGC CCAGAGAGAC
    AACGCTGGGG ACCGGGCAGA TTTCTGGAGA ATGCGTGGCC AGAGGTCTGG GGTGAAGAAG
    AACCTGAGGA AAAGTCGGGA AGACCTCACA GCTGTTGTGT CTGTCAGCAC CAAGTTCCCA
    GCTTATGAGA GGGTTTTGCT GCGAGAAGCT GGTTTCAAGA GACCTGTGGT CTTATTCGGC
    CCCATAGCTG ATATAGCAAT GGAAAAATTG GCTAATGAGT TACCTGACTG GTTTCAAACT
    GCTAAAACGG AACCAAAAGA TGCAGGATCT GAGAAATCCA CTGGAGTGGT CCGGTTAAAT
    ACCGTGAGGC AAATTATTGA ACAGGATAAG CATGCACTAC TGGATGTGAC TCCGAAAGCT
    GTGGACCTGT TGAATTACAC CCAGTGGTTC CCAATTGTGA TTTTTTTCAA CCCAGACTCC
    AGACAAGGTG TCAAAACCAT GAGACAAAGG TTAAATCCAA CGTCCAACAA AAGTTCTCGA
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACGTGTG CACACCTTTT TACAGCTACA
    ATCAACCTAA ATTCAGCCAA TGATAGCTGG TTTGGCAGCT TAAAGGACAC TATTCAGCAT
    CAGCAAGGAG AAGCGGTTTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGACCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACGGACGGT GAAGGAGGCG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CCGAGGAGCC GCTGGTGTCG TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CACGAGGAGA GCATAAGGAA ACCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT
    AGCAGCGATC AACTTAGGGA CAATAGCCCG CCCCCAGCAT TCAAGCCAGA GCCGCCCAAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870815.1
    CDS148..3789
    Translation

    Target ORF information:

    RefSeq Version XM_017015326.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015326.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGCTGGGG TGCGAAGTAC CACATTACTG TCATCTCCCT GTGAGTGGGA TTGGCACCCC 
    GGCTGCAGCT CCAGGAGCAA GTACTCCAGG AAGCACAGAG CTGTACCAGT ACACGGCCCA
    CGTTGCCAGG CCCCAGGCAT GGAAGAGCTG ATATGGGAAC AGTACACTGT GACCCTACAA
    AAGGATTCCA AAAGAGGATT TGGAATTGCA GTGTCCGGAG GCAGAGACAA CCCCCACTTT
    GAAAATGGAG AAACGTCAAT TGTCATTTCT GATGTGCTCC CGGGTGGGCC TGCTGATGGG
    CTGCTCCAAG AAAATGACAG AGTGGTCATG GTCAATGGCA CCCCCATGGA GGATGTGCTT
    CATTCGTTTG CAGTTCAGCA GCTCAGAAAA AGTGGGAAGG TCGCTGCTAT TGTGGTCAAG
    AGGCCCCGGA AGGTCCAGGT GGCCGCACTT CAGGCCAGCC CTCCCCTGGA TCAGGATGAC
    CGGGCTTTTG AGGTGATGGA CGAGTTTGAT GGCAGAAGTT TCCGGAGTGG CTACAGCGAG
    AGGAGCCGGC TGAACAGCCA TGGGGGGCGC AGCCGCAGCT GGGAGGACAG CCCGGAAAGG
    GGGCGTCCCC ATGAGCGGGC CCGGAGCCGG GAGCGGGACC TCAGCCGGGA CCGGAGCCGT
    GGCCGGAGCC TGGAGCGGGG CCTGGACCAA GACCATGCGC GCACCCGAGA CCGCAGCCGT
    GGCCGGAGCC TGGAGCGGGG CCTGGACCAC GACTTTGGGC CATCCCGGGA CCGGGACCGT
    GACCGCAGCC GCGGCCGGAG CATTGACCAG GACTACGAGC GAGCCTATCA CCGGGCCTAC
    GACCCAGACT ACGAGCGGGC CTACAGCCCG GAGTACAGGC GCGGGGCCCG CCACGATGCC
    CGCTCTCGGG GACCCCGAAG CCGCAGCCGC GAGCACCCGC ACTCACGGAG CCCCAGCCCC
    GAGCCTAGGG GGCGGCCGGG GCCCATCGGG GTCCTCCTGA TGAAAAGCAG AGCGAACGAA
    GAGTATGGTC TCCGGCTTGG GAGTCAGATC TTCGTAAAGG AAATGACCCG AACGGGTCTG
    GCAACTAAAG ATGGCAACCT TCACGAAGGA GACATAATTC TCAAGATCAA TGGGACTGTA
    ACTGAGAACA TGTCTTTAAC GGATGCTCGA AAATTGATAG AAAAGTCAAG AGGAAAACTA
    CAGCTAGTGG TGTTGAGAGA CAGCCAGCAG ACCCTCATCA ACATCCCGTC ATTAAATGAC
    AGTGACTCAG AAATAGAAGA TATTTCAGAA ATAGAGTCAA ACCGATCATT TTCTCCAGAG
    GAGAGACGTC ATCAGTATTC TGATTATGAT TATCATTCCT CAAGTGAGAA GCTGAAGGAA
    AGGCCAAGTT CCAGAGAGGA CACGCCGAGC AGATTGTCCA GGATGGGTGC GACACCCACT
    CCCTTTAAGT CCACAGGGGA TATTGCAGGC ACAGTTGTCC CAGAGACCAA CAAGGAACCC
    AGATACCAAG AGGACCCCCC AGCTCCTCAA CCAAAAGCAG CCCCGAGAAC TTTTCTTCGT
    CCTAGTCCTG AAGATGAAGC AATATATGGC CCTAATACCA AAATGGTAAG GTTCAAGAAG
    GGAGACAGCG TGGGCCTCCG GTTGGCTGGT GGCAATGATG TCGGGATATT TGTTGCTGGC
    ATTCAAGAAG GGACCTCGGC GGAGCAGGAG GGCCTTCAAG AAGGAGACCA GATTCTGAAG
    GTGAACACAC AGGATTTCAG AGGATTAGTG CGGGAGGATG CCGTTCTCTA CCTGTTAGAA
    ATCCCTAAAG GTGAAATGGT GACCATTTTA GCTCAGAGCC GAGCCGATGT GTATAGAGAC
    ATCCTGGCTT GTGGCAGAGG GGATTCGTTT TTTATAAGAA GCCACTTTGA ATGTGAGAAG
    GAAACTCCAC AGAGCCTGGC CTTCACCAGA GGGGAGGTCT TCCGAGTGGT AGACACACTG
    TATGACGGCA AGCTGGGCAA CTGGCTGGCT GTGAGGATTG GGAACGAGTT GGAGAAAGGC
    TTAATCCCCA ACAAGAGCAG AGCTGAACAA ATGGCCAGTG TTCAAAATGC CCAGAGAGAC
    AACGCTGGGG ACCGGGCAGA TTTCTGGAGA ATGCGTGGCC AGAGGTCTGG GGTGAAGAAG
    AACCTGAGGA AAAGTCGGGA AGACCTCACA GCTGTTGTGT CTGTCAGCAC CAAGTTCCCA
    GCTTATGAGA GGGTTTTGCT GCGAGAAGCT GGTTTCAAGA GACCTGTGGT CTTATTCGGC
    CCCATAGCTG ATATAGCAAT GGAAAAATTG GCTAATGAGT TACCTGACTG GTTTCAAACT
    GCTAAAACGG AACCAAAAGA TGCAGGATCT GAGAAATCCA CTGGAGTGGT CCGGTTAAAT
    ACCGTGAGGC AAATTATTGA ACAGGATAAG CATGCACTAC TGGATGTGAC TCCGAAAGCT
    GTGGACCTGT TGAATTACAC CCAGTGGTTC CCAATTGTGA TTTTTTTCAA CCCAGACTCC
    AGACAAGGTG TCAAAACCAT GAGACAAAGG TTAAATCCAA CGTCCAACAA AAGTTCTCGA
    AAGTTATTTG ATCAAGCCAA CAAGCTTAAA AAAACGTGTG CACACCTTTT TACAGCTACA
    ATCAACCTAA ATTCAGCCAA TGATAGCTGG TTTGGCAGCT TAAAGGACAC TATTCAGCAT
    CAGCAAGGAG AAGCGGTTTG GGTCTCTGAA GGAAAGATGG AAGGGATGGA TGATGACCCC
    GAAGACCGCA TGTCCTACTT AACCGCCATG GGCGCGGACT ATCTGAGTTG CGACAGCCGC
    CTCATCAGTG ACTTTGAAGA CACGGACGGT GAAGGAGGCG CCTACACTGA CAATGAGCTG
    GATGAGCCAG CCGAGGAGCC GCTGGTGTCG TCCATCACCC GCTCCTCGGA GCCGGTGCAG
    CACGAGGAGA GCATAAGGAA ACCCAGCCCA GAGCCACGAG CTCAGATGAG GAGGGCTGCT
    AGCAGCGATC AACTTAGGGA CAATAGCCCG CCCCCAGCAT TCAAGCCAGA GCCGCCCAAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99018
    Clone ID Related Accession (Same CDS sequence) XM_017015327.2 , NM_001369872.1 , XM_017015327.1
    Accession Version XM_017015327.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017015327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870816.1
    CDS137..3598
    Translation

    Target ORF information:

    RefSeq Version XM_017015327.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015327.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99018
    Clone ID Related Accession (Same CDS sequence) XM_017015327.2 , NM_001369872.1 , XM_017015327.1
    Accession Version XM_017015327.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3462bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870816.1
    CDS319..3780
    Translation

    Target ORF information:

    RefSeq Version XM_017015327.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCCGGTGC GAGGAGACCG CGGGTTTCCA CCCCGGCGGG AGCTGTCAGG TTGGCTCCGC 
    GCCCCAGGCA TGGAAGAGCT GATATGGGAA CAGTACACTG TGACCCTACA AAAGGATTCC
    AAAAGAGGAT TTGGAATTGC AGTGTCCGGA GGCAGAGACA ACCCCCACTT TGAAAATGGA
    GAAACGTCAA TTGTCATTTC TGATGTGCTC CCGGGTGGGC CTGCTGATGG GCTGCTCCAA
    GAAAATGACA GAGTGGTCAT GGTCAATGGC ACCCCCATGG AGGATGTGCT TCATTCGTTT
    GCAGTTCAGC AGCTCAGAAA AAGTGGGAAG GTCGCTGCTA TTGTGGTCAA GAGGCCCCGG
    AAGGTCCAGG TGGCCGCACT TCAGGCCAGC CCTCCCCTGG ATCAGGATGA CCGGGCTTTT
    GAGGTGATGG ACGAGTTTGA TGGCAGAAGT TTCCGGAGTG GCTACAGCGA GAGGAGCCGG
    CTGAACAGCC ATGGGGGGCG CAGCCGCAGC TGGGAGGACA GCCCGGAAAG GGGGCGTCCC
    CATGAGCGGG CCCGGAGCCG GGAGCGGGAC CTCAGCCGGG ACCGGAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA AGACCATGCG CGCACCCGAG ACCGCAGCCG TGGCCGGAGC
    CTGGAGCGGG GCCTGGACCA CGACTTTGGG CCATCCCGGG ACCGGGACCG TGACCGCAGC
    CGCGGCCGGA GCATTGACCA GGACTACGAG CGAGCCTATC ACCGGGCCTA CGACCCAGAC
    TACGAGCGGG CCTACAGCCC GGAGTACAGG CGCGGGGCCC GCCACGATGC CCGCTCTCGG
    GGACCCCGAA GCCGCAGCCG CGAGCACCCG CACTCACGGA GCCCCAGCCC CGAGCCTAGG
    GGGCGGCCGG GGCCCATCGG GGTCCTCCTG ATGAAAAGCA GAGCGAACGA AGAGTATGGT
    CTCCGGCTTG GGAGTCAGAT CTTCGTAAAG GAAATGACCC GAACGGGTCT GGCAACTAAA
    GATGGCAACC TTCACGAAGG AGACATAATT CTCAAGATCA ATGGGACTGT AACTGAGAAC
    ATGTCTTTAA CGGATGCTCG AAAATTGATA GAAAAGTCAA GAGGAAAACT ACAGCTAGTG
    GTGTTGAGAG ACAGCCAGCA GACCCTCATC AACATCCCGT CATTAAATGA CAGTGACTCA
    GAAATAGAAG ATATTTCAGA AATAGAGTCA AACCGATCAT TTTCTCCAGA GGAGAGACGT
    CATCAGTATT CTGATTATGA TTATCATTCC TCAAGTGAGA AGCTGAAGGA AAGGCCAAGT
    TCCAGAGAGG ACACGCCGAG CAGATTGTCC AGGATGGGTG CGACACCCAC TCCCTTTAAG
    TCCACAGGGG ATATTGCAGG CACAGTTGTC CCAGAGACCA ACAAGGAACC CAGATACCAA
    GAGGACCCCC CAGCTCCTCA ACCAAAAGCA GCCCCGAGAA CTTTTCTTCG TCCTAGTCCT
    GAAGATGAAG CAATATATGG CCCTAATACC AAAATGGTAA GGTTCAAGAA GGGAGACAGC
    GTGGGCCTCC GGTTGGCTGG TGGCAATGAT GTCGGGATAT TTGTTGCTGG CATTCAAGAA
    GGGACCTCGG CGGAGCAGGA GGGCCTTCAA GAAGGAGACC AGATTCTGAA GGTGAACACA
    CAGGATTTCA GAGGATTAGT GCGGGAGGAT GCCGTTCTCT ACCTGTTAGA AATCCCTAAA
    GGTGAAATGG TGACCATTTT AGCTCAGAGC CGAGCCGATG TGTATAGAGA CATCCTGGCT
    TGTGGCAGAG GGGATTCGTT TTTTATAAGA AGCCACTTTG AATGTGAGAA GGAAACTCCA
    CAGAGCCTGG CCTTCACCAG AGGGGAGGTC TTCCGAGTGG TAGACACACT GTATGACGGC
    AAGCTGGGCA ACTGGCTGGC TGTGAGGATT GGGAACGAGT TGGAGAAAGG CTTAATCCCC
    AACAAGAGCA GAGCTGAACA AATGGCCAGT GTTCAAAATG CCCAGAGAGA CAACGCTGGG
    GACCGGGCAG ATTTCTGGAG AATGCGTGGC CAGAGGTCTG GGGTGAAGAA GAACCTGAGG
    AAAAGTCGGG AAGACCTCAC AGCTGTTGTG TCTGTCAGCA CCAAGTTCCC AGCTTATGAG
    AGGGTTTTGC TGCGAGAAGC TGGTTTCAAG AGACCTGTGG TCTTATTCGG CCCCATAGCT
    GATATAGCAA TGGAAAAATT GGCTAATGAG TTACCTGACT GGTTTCAAAC TGCTAAAACG
    GAACCAAAAG ATGCAGGATC TGAGAAATCC ACTGGAGTGG TCCGGTTAAA TACCGTGAGG
    CAAATTATTG AACAGGATAA GCATGCACTA CTGGATGTGA CTCCGAAAGC TGTGGACCTG
    TTGAATTACA CCCAGTGGTT CCCAATTGTG ATTTTTTTCA ACCCAGACTC CAGACAAGGT
    GTCAAAACCA TGAGACAAAG GTTAAATCCA ACGTCCAACA AAAGTTCTCG AAAGTTATTT
    GATCAAGCCA ACAAGCTTAA AAAAACGTGT GCACACCTTT TTACAGCTAC AATCAACCTA
    AATTCAGCCA ATGATAGCTG GTTTGGCAGC TTAAAGGACA CTATTCAGCA TCAGCAAGGA
    GAAGCGGTTT GGGTCTCTGA AGGAAAGATG GAAGGGATGG ATGATGACCC CGAAGACCGC
    ATGTCCTACT TAACCGCCAT GGGCGCGGAC TATCTGAGTT GCGACAGCCG CCTCATCAGT
    GACTTTGAAG ACACGGACGG TGAAGGAGGC GCCTACACTG ACAATGAGCT GGATGAGCCA
    GCCGAGGAGC CGCTGGTGTC GTCCATCACC CGCTCCTCGG AGCCGGTGCA GCACGAGGAG
    GCCAAAACCC AGAACAAAGA AGAATCCTAT GACTTCTCCA AATCCTATGA ATATAAGTCA
    AACCCCTCTG CCGTTGCTGG TAATGAAACT CCTGGGGCAT CTACCAAAGG TTATCCTCCT
    CCTGTTGCAG CAAAACCTAC CTTTGGGCGG TCTATACTGA AGCCCTCCAC TCCCATCCCT
    CCTCAAGAGG GTGAGGAGGT GGGAGAGAGC AGTGAGGAGC AAGATAATGC TCCCAAATCA
    GTCCTGGGCA AAGTCAAAAT ATTTGAGAAG ATGGATCACA AGGCCAGGTT ACAGAGAATG
    CAGGAGCTCC AGGAAGCACA GAATGCAAGG ATCGAAATTG CCCAGAAGCA TCCTGATATC
    TATGCAGTTC CAATCAAAAC GCACAAGCCA GACCCTGGCA CGCCCCAGCA CACGAGTTCC
    AGACCCCCTG AGCCACAGAA AGCTCCTTCC AGACCTTATC AGGATACCAG AGGAAGTTAT
    GGCAGTGATG CCGAGGAGGA GGAGTACCGC CAGCAGCTGT CAGAACACTC CAAGCGCGGT
    TACTATGGCC AGTCTGCCCG ATACCGGGAC ACAGAATTAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99019
    Clone ID Related Accession (Same CDS sequence) XM_017015328.1 , NM_001369874.1
    Accession Version XM_017015328.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product tight junction protein ZO-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016870817.1
    CDS360..3503
    Translation

    Target ORF information:

    RefSeq Version XM_017015328.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 2 (TJP2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017015328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAAGACTG CTCAAGCCCT ACATAGGATG TGGATCCAGG CTGTTAAAAA GTTGAGGAGA 
    TGGAAAGGCC GTGCCCCAGG CATGGAAGAG CTGATATGGG AACAGTACAC TGTGACCCTA
    CAAAAGGATT CCAAAAGAGG ATTTGGAATT GCAGTGTCCG GAGGCAGAGA CAACCCCCAC
    TTTGAAAATG GAGAAACGTC AATTGTCATT TCTGATGTGC TCCCGGGTGG GCCTGCTGAT
    GGGCTGCTCC AAGAAAATGA CAGAGTGGTC ATGGTCAATG GCACCCCCAT GGAGGATGTG
    CTTCATTCGT TTGCAGTTCA GCAGCTCAGA AAAAGTGGGA AGGTCGCTGC TATTGTGGTC
    AAGAGGCCCC GGAAGGTCCA GGTGGCCGCA CTTCAGGCCA GCCCTCCCCT GGATCAGGAT
    GACCGGGCTT TTGAGGTGAT GGACGAGTTT GATGGCAGAA GTTTCCGGAG TGGCTACAGC
    GAGAGGAGCC GGCTGAACAG CCATGGGGGG CGCAGCCGCA GCTGGGAGGA CAGCCCGGAA
    AGGGGGCGTC CCCATGAGCG GGCCCGGAGC CGGGAGCGGG ACCTCAGCCG GGACCGGAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CAAGACCATG CGCGCACCCG AGACCGCAGC
    CGTGGCCGGA GCCTGGAGCG GGGCCTGGAC CACGACTTTG GGCCATCCCG GGACCGGGAC
    CGTGACCGCA GCCGCGGCCG GAGCATTGAC CAGGACTACG AGCGAGCCTA TCACCGGGCC
    TACGACCCAG ACTACGAGCG GGCCTACAGC CCGGAGTACA GGCGCGGGGC CCGCCACGAT
    GCCCGCTCTC GGGGACCCCG AAGCCGCAGC CGCGAGCACC CGCACTCACG GAGCCCCAGC
    CCCGAGCCTA GGGGGCGGCC GGGGCCCATC GGGGTCCTCC TGATGAAAAG CAGAGCGAAC
    GAAGAGTATG GTCTCCGGCT TGGGAGTCAG ATCTTCGTAA AGGAAATGAC CCGAACGGGT
    CTGGCAACTA AAGATGGCAA CCTTCACGAA GGAGACATAA TTCTCAAGAT CAATGGGACT
    GTAACTGAGA ACATGTCTTT AACGGATGCT CGAAAATTGA TAGAAAAGTC AAGAGGAAAA
    CTACAGCTAG TGGTGTTGAG AGACAGCCAG CAGACCCTCA TCAACATCCC GTCATTAAAT
    GACAGTGACT CAGAAATAGA AGATATTTCA GAAATAGAGT CAAACCGATC ATTTTCTCCA
    GAGGAGAGAC GTCATCAGTA TTCTGATTAT GATTATCATT CCTCAAGTGA GAAGCTGAAG
    GAAAGGCCAA GTTCCAGAGA GGACACGCCG AGCAGATTGT CCAGGATGGG TGCGACACCC
    ACTCCCTTTA AGTCCACAGG GGATATTGCA GGCACAGTTG TCCCAGAGAC CAACAAGGAA
    CCCAGATACC AAGAGGACCC CCCAGCTCCT CAACCAAAAG CAGCCCCGAG AACTTTTCTT
    CGTCCTAGTC CTGAAGATGA AGCAATATAT GGCCCTAATA CCAAAATGGT AAGGTTCAAG
    AAGGGAGACA GCGTGGGCCT CCGGTTGGCT GGTGGCAATG ATGTCGGGAT ATTTGTTGCT
    GGCATTCAAG AAGGGACCTC GGCGGAGCAG GAGGGCCTTC AAGAAGGAGA CCAGATTCTG
    AAGGTGAACA CACAGGATTT CAGAGGATTA GTGCGGGAGG ATGCCGTTCT CTACCTGTTA
    GAAATCCCTA AAGGTGAAAT GGTGACCATT TTAGCTCAGA GCCGAGCCGA TGTGTATAGA
    GACATCCTGG CTTGTGGCAG AGGGGATTCG TTTTTTATAA GAAGCCACTT TGAATGTGAG
    AAGGAAACTC CACAGAGCCT GGCCTTCACC AGAGGGGAGG TCTTCCGAGT GGTAGACACA
    CTGTATGACG GCAAGCTGGG CAACTGGCTG GCTGTGAGGA TTGGGAACGA GTTGGAGAAA
    GGCTTAATCC CCAACAAGAG CAGAGCTGAA CAAATGGCCA GTGTTCAAAA TGCCCAGAGA
    GACAACGCTG GGGACCGGGC AGATTTCTGG AGAATGCGTG GCCAGAGGTC TGGGGTGAAG
    AAGAACCTGA GGAAAAGTCG GGAAGACCTC ACAGCTGTTG TGTCTGTCAG CACCAAGTTC
    CCAGCTTATG AGAGGGTTTT GCTGCGAGAA GCTGGTTTCA AGAGACCTGT GGTCTTATTC
    GGCCCCATAG CTGATATAGC AATGGAAAAA TTGGCTAATG AGTTACCTGA CTGGTTTCAA
    ACTGCTAAAA CGGAACCAAA AGATGCAGGA TCTGAGAAAT CCACTGGAGT GGTCCGGTTA
    AATACCGTGA GGCAAATTAT TGAACAGGAT AAGCATGCAC TACTGGATGT GACTCCGAAA
    GCTGTGGACC TGTTGAATTA CACCCAGTGG TTCCCAATTG TGATTTTTTT CAACCCAGAC
    TCCAGACAAG GTGTCAAAAC CATGAGACAA AGGTTAAATC CAACGTCCAA CAAAAGTTCT
    CGAAAGTTAT TTGATCAAGC CAACAAGCTT AAAAAAACGT GTGCACACCT TTTTACAGCT
    ACAATCAACC TAAATTCAGC CAATGATAGC TGGTTTGGCA GCTTAAAGGA CACTATTCAG
    CATCAGCAAG GAGAAGCGGT TTGGGTCTCT GAAGGAAAGA TGGAAGGGAT GGATGATGAC
    CCCGAAGACC GCATGTCCTA CTTAACCGCC ATGGGCGCGG ACTATCTGAG TTGCGACAGC
    CGCCTCATCA GTGACTTTGA AGACACGGAC GGTGAAGGAG GCGCCTACAC TGACAATGAG
    CTGGATGAGC CAGCCGAGGA GCCGCTGGTG TCGTCCATCA CCCGCTCCTC GGAGCCGGTG
    CAGCACGAGG AGATCGAAAT TGCCCAGAAG CATCCTGATA TCTATGCAGT TCCAATCAAA
    ACGCACAAGC CAGACCCTGG CACGCCCCAG CACACGAGTT CCAGACCCCC TGAGCCACAG
    AAAGCTCCTT CCAGACCTTA TCAGGATACC AGAGGAAGTT ATGGCAGTGA TGCCGAGGAG
    GAGGAGTACC GCCAGCAGCT GTCAGAACAC TCCAAGCGCG GTTACTATGG CCAGTCTGCC
    CGATACCGGG ACACAGAATT ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.