MED21 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED21
Entrez Gene ID	9412
Full Name	mediator complex subunit 21
Synonyms	SRB7,SURB7,hSrb7
General protein information	Preferred Names mediator complex subunit 21 Names mediator of RNA polymerase II transcription subunit 21 RNA polymerase II holoenzyme component SRB7 RNAPII complex component SRB7 SRB7 suppressor of RNA polymerase B homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p11.23
Summary	This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012].
Disorder MIM:	603800

mRNA and Protein(s)

mRNA	Protein	Name
NM_004264.4	NP_004255.2	mediator of RNA polymerase II transcription subunit 21 isoform 1
NM_001271811.1	NP_001258740.1	mediator of RNA polymerase II transcription subunit 21 isoform 2
NM_004264.5	NP_004255.2	mediator of RNA polymerase II transcription subunit 21 isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MED21	XP_001143632.1
Canis lupus familiaris (dog)	MED21	NP_001184240.1
Bos taurus (cattle)	MED21	NP_001033655.1
Mus musculus (house mouse)	Med21	NP_079591.1
	MED21	XP_001099368.1
Anopheles gambiae (African malaria mosquito)	MED21_ANOGA	XP_307937.5
Xenopus tropicalis (tropical clawed frog)	med21	NP_001165322.1
Homo sapiens (human)	MED21	NP_004255.2
Gallus gallus (chicken)	MED21	XP_416442.2
Drosophila melanogaster (fruit fly)	MED21	NP_001015223.1
Rattus norvegicus (Norway rat)	Med21	NP_001101365.2
Danio rerio (zebrafish)	med21	NP_998588.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

Mediator acts upstream of the transcriptional activator Gal4.
Ang K, Ee G, Ang E, Koh E, Siew WL, Chan YM, Nur S, Tan YS, Lehming N
PLoS biology10(3)e1001290(2012)

The transcriptional coactivator DRIP/mediator complex is involved in vitamin D receptor function and regulates keratinocyte proliferation and differentiation.
Oda Y, Chalkley RJ, Burlingame AL, Bikle DD
The Journal of investigative dermatology130(10)2377-88(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Blocking MED1/MED21 expression caused hyperproliferation of keratinocytes
Title: The transcriptional coactivator DRIP/mediator complex is involved in vitamin D receptor function and regulates keratinocyte proliferation and differentiation.

Co-expressed yeast MED21 and MED7 in E. coli to determine structure.
Title: A conserved mediator hinge revealed in the structure of the MED7.MED21 (Med7.Srb7) heterodimer.

The following MED21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00172	NM_004264.5 Latest version!	Homo sapiens mediator complex subunit 21 (MED21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00209	NM_001271811.1 Latest version!	Homo sapiens mediator complex subunit 21 (MED21), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu00172	NM_004264.4	Homo sapiens mediator complex subunit 21 (MED21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED21 ( NM_004264.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004255.2 Homo sapiens mediator complex subunit 21 (MED21), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00172
Clone ID Related Accession (Same CDS sequence)	NM_004264.4 , NM_004264.5
Accession Version	NM_004264.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 21 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB024509.1, BC008380.1 and AC092747.10. On Nov 24, 2012 this sequence version replaced NM_004264.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.94015.1, SRR1803617.271031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATC GGCTCACGCA GCTTCAGGAC GCTGTGAATT CGCTTGCAGA TCAGTTTTGT 
AATGCCATTG GAGTATTGCA GCAATGTGGT CCTCCTGCCT CTTTCAATAA TATTCAGACA 
GCAATTAACA AAGACCAGCC AGCTAACCCT ACAGAAGAGT ATGCCCAGCT TTTTGCAGCA 
CTGATTGCAC GAACAGCAAA AGACATTGAT GTTTTGATAG ATTCCTTACC CAGTGAAGAA 
TCTACAGCTG CTTTACAGGC TGCTAGCTTG TATAAGCTAG AAGAAGAAAA CCATGAAGCT 
GCTACATGTC TGGAGGATGT TGTTTATCGA GGAGACATGC TTCTGGAGAA GATACAAAGC 
GCACTTGCTG ATATTGCACA GTCACAGCTG AAGACAAGAA GTGGTACCCA TAGCCAGTCT 
CTTCCAGACT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004255.2
CDS	59..493
Translation	MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS

Target ORF information:

RefSeq Version	NM_004264.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 21 (MED21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004264.4

ORF Insert Sequence:

ATGGCGGATC GGCTCACGCA GCTTCAGGAC GCTGTGAATT CGCTTGCAGA TCAGTTTTGT 
AATGCCATTG GAGTATTGCA GCAATGTGGT CCTCCTGCCT CTTTCAATAA TATTCAGACA 
GCAATTAACA AAGACCAGCC AGCTAACCCT ACAGAAGAGT ATGCCCAGCT TTTTGCAGCA 
CTGATTGCAC GAACAGCAAA AGACATTGAT GTTTTGATAG ATTCCTTACC CAGTGAAGAA 
TCTACAGCTG CTTTACAGGC TGCTAGCTTG TATAAGCTAG AAGAAGAAAA CCATGAAGCT 
GCTACATGTC TGGAGGATGT TGTTTATCGA GGAGACATGC TTCTGGAGAA GATACAAAGC 
GCACTTGCTG ATATTGCACA GTCACAGCTG AAGACAAGAA GTGGTACCCA TAGCCAGTCT 
CTTCCAGACT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED21 ( NM_004264.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004255.2 Homo sapiens mediator complex subunit 21 (MED21), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00172
Clone ID Related Accession (Same CDS sequence)	NM_004264.4 , NM_004264.5
Accession Version	NM_004264.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 21 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092747.10. On Nov 23, 2018 this sequence version replaced NM_004264.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.224775.1, SRR3476690.681190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000282892.4/ ENSP00000282892.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGATC GGCTCACGCA GCTTCAGGAC GCTGTGAATT CGCTTGCAGA TCAGTTTTGT 
AATGCCATTG GAGTATTGCA GCAATGTGGT CCTCCTGCCT CTTTCAATAA TATTCAGACA 
GCAATTAACA AAGACCAGCC AGCTAACCCT ACAGAAGAGT ATGCCCAGCT TTTTGCAGCA 
CTGATTGCAC GAACAGCAAA AGACATTGAT GTTTTGATAG ATTCCTTACC CAGTGAAGAA 
TCTACAGCTG CTTTACAGGC TGCTAGCTTG TATAAGCTAG AAGAAGAAAA CCATGAAGCT 
GCTACATGTC TGGAGGATGT TGTTTATCGA GGAGACATGC TTCTGGAGAA GATACAAAGC 
GCACTTGCTG ATATTGCACA GTCACAGCTG AAGACAAGAA GTGGTACCCA TAGCCAGTCT 
CTTCCAGACT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004255.2
CDS	23..457
Translation	MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEEYAQLFAALIARTAKDIDVLIDSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALADIAQSQLKTRSGTHSQSLPDS

Target ORF information:

RefSeq Version	NM_004264.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 21 (MED21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004264.5

ORF Insert Sequence:

ATGGCGGATC GGCTCACGCA GCTTCAGGAC GCTGTGAATT CGCTTGCAGA TCAGTTTTGT 
AATGCCATTG GAGTATTGCA GCAATGTGGT CCTCCTGCCT CTTTCAATAA TATTCAGACA 
GCAATTAACA AAGACCAGCC AGCTAACCCT ACAGAAGAGT ATGCCCAGCT TTTTGCAGCA 
CTGATTGCAC GAACAGCAAA AGACATTGAT GTTTTGATAG ATTCCTTACC CAGTGAAGAA 
TCTACAGCTG CTTTACAGGC TGCTAGCTTG TATAAGCTAG AAGAAGAAAA CCATGAAGCT 
GCTACATGTC TGGAGGATGT TGTTTATCGA GGAGACATGC TTCTGGAGAA GATACAAAGC 
GCACTTGCTG ATATTGCACA GTCACAGCTG AAGACAAGAA GTGGTACCCA TAGCCAGTCT 
CTTCCAGACT CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED21 ( NM_001271811.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258740.1 Homo sapiens mediator complex subunit 21 (MED21), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00209
Clone ID Related Accession (Same CDS sequence)	NM_001271811.1
Accession Version	NM_001271811.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 21 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB024509.1, HY075642.1 and AC092747.10. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.234782.1, SRR1803617.416455.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2145893 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGATC GGCTCACGCA GCTTCAGGAC GCTGTGAATT CGCTTGCAGA TCAGTTTTGT 
AATGCCATTG GAGTATTGCA GCAATGTGGT CCTCCTGCCT CTTTCAATAA TATTCAGACA 
GCAATTAACA AAGACCAGCC AGCTAACCCT ACAGAAGTAT GCCCAGCTTT TTGCAGCACT 
GATTGCACGA ACAGCAAAAG ACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258740.1
CDS	59..265
Translation	MADRLTQLQDAVNSLADQFCNAIGVLQQCGPPASFNNIQTAINKDQPANPTEVCPAFCSTDCTNSKRH

Target ORF information:

RefSeq Version	NM_001271811.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 21 (MED21), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271811.1

ORF Insert Sequence:

ATGGCGGATC GGCTCACGCA GCTTCAGGAC GCTGTGAATT CGCTTGCAGA TCAGTTTTGT 
AATGCCATTG GAGTATTGCA GCAATGTGGT CCTCCTGCCT CTTTCAATAA TATTCAGACA 
GCAATTAACA AAGACCAGCC AGCTAACCCT ACAGAAGTAT GCCCAGCTTT TTGCAGCACT 
GATTGCACGA ACAGCAAAAG ACATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

Mediator acts upstream of the transcriptional activator Gal4.
PLoS biology10(3)e1001290(2012)
Ang K,Ee G,Ang E,Koh E,Siew WL,Chan YM,Nur S,Tan YS,Lehming N

The transcriptional coactivator DRIP/mediator complex is involved in vitamin D receptor function and regulates keratinocyte proliferation and differentiation.
The Journal of investigative dermatology130(10)2377-88(2010 Oct)
Oda Y,Chalkley RJ,Burlingame AL,Bikle DD

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MED21 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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