PIGS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIGS
Entrez Gene ID	94005
Full Name	phosphatidylinositol glycan anchor biosynthesis class S
General protein information	Preferred Names phosphatidylinositol glycan anchor biosynthesis class S Names GPI transamidase component PIG-S GPI transamidase subunit phosphatidylinositol glycan, class S phosphatidylinositol-glycan biosynthesis class S protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q11.2
Summary	This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins. [provided by RefSeq, Jul 2008].
Disorder MIM:	610271

mRNA and Protein(s)

mRNA	Protein	Name
NM_033198.3	NP_149975.1	GPI transamidase component PIG-S
NM_033198.4	NP_149975.1	GPI transamidase component PIG-S

Pathways from BioSystems

Homologies

Homo sapiens (human)	PIGS	NP_149975.1
	PIGS	XP_001107461.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002933	XP_311969.5
Kluyveromyces lactis	KLLA0B01045g	XP_451577.1
Neurospora crassa	NCU06033	XP_959623.1
Rattus norvegicus (Norway rat)	Pigs	NP_001006602.1
Danio rerio (zebrafish)	pigs	NP_001070082.1
Schizosaccharomyces pombe (fission yeast)	gpi17	NP_594335.1
Pan troglodytes (chimpanzee)	PIGS	XP_511797.2
Saccharomyces cerevisiae (baker's yeast)	GPI17	NP_010722.3
Mus musculus (house mouse)	Pigs	NP_958808.1
Gallus gallus (chicken)	PIGS	XP_415817.4
Eremothecium gossypii	AGOS_ADR180C	NP_984276.1
Xenopus tropicalis (tropical clawed frog)	pigs	NP_001106424.1
Canis lupus familiaris (dog)	PIGS	XP_548290.4
Bos taurus (cattle)	PIGS	NP_001029388.1
Drosophila melanogaster (fruit fly)	CG31120	NP_733060.1
Magnaporthe oryzae (rice blast fungus)	MGG_08259	XP_003715825.1
Arabidopsis thaliana (thale cress)	AT3G07180	NP_187374.2
Oryza sativa (rice)	Os01g0907300	NP_001045136.1

Gene Ontology
Bibliography

Related articles in PubMed

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex.
Vainauskas S, Menon AK
The Journal of biological chemistry280(16)16402-9(2005 Apr)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.
Hong Y, Ohishi K, Kang JY, Tanaka S, Inoue N, Nishimura J, Maeda Y, Kinoshita T
Molecular biology of the cell14(5)1780-9(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PIGS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIGS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05365	NM_033198.4 Latest version!	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class S (PIGS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu05365	NM_033198.3	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class S (PIGS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIGS ( NM_033198.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_149975.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class S (PIGS), mRNA.

Price & Availability↑

CloneID	OHu05365
Clone ID Related Accession (Same CDS sequence)	NM_033198.3 , NM_033198.4
Accession Version	NM_033198.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	GPI transamidase component PIG-S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA591284.1, AK075428.1, BC007301.1 and AW084841.1. On Nov 7, 2008 this sequence version replaced NM_033198.2. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.86390.1, SRR1803613.25959.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCCG CCGGGGCTGC GGCTACACAC CTAGAGGTGG CCCGGGGCAA GCGCGCCGCC 
CTCTTCTTCG CTGCGGTGGC CATCGTGCTG GGGCTACCGC TCTGGTGGAA GACCACGGAG 
ACCTACCGGG CCTCGTTGCC TTACTCCCAG ATCAGTGGCC TGAATGCCCT TCAGCTCCGC 
CTCATGGTGC CTGTCACTGT CGTGTTTACG CGGGAGTCAG TGCCCCTGGA CGACCAGGAG 
AAGCTGCCCT TCACCGTTGT GCATGAAAGA GAGATTCCTC TGAAATACAA AATGAAAATC 
AAATGCCGTT TCCAGAAGGC CTATCGGAGG GCTTTGGACC ATGAGGAGGA GGCCCTGTCA 
TCGGGCAGTG TGCAAGAGGC AGAAGCCATG TTAGATGAGC CTCAGGAACA AGCGGAGGGC 
TCCCTGACTG TGTACGTGAT ATCTGAACAC TCCTCACTTC TTCCCCAGGA CATGATGAGC 
TACATTGGGC CCAAGAGGAC AGCAGTGGTG CGGGGGATAA TGCACCGGGA GGCCTTTAAC 
ATCATTGGCC GCCGCATAGT CCAGGTGGCC CAGGCCATGT CTTTGACTGA GGATGTGCTT 
GCTGCTGCTC TGGCTGACCA CCTTCCAGAG GACAAGTGGA GCGCTGAGAA GAGGCGGCCT 
CTCAAGTCCA GCTTGGGCTA TGAGATCACC TTCAGTTTAC TCAACCCAGA CCCCAAGTCC 
CATGATGTCT ACTGGGACAT TGAGGGGGCT GTCCGGCGCT ATGTGCAACC TTTCCTGAAT 
GCCCTCGGTG CCGCTGGCAA CTTCTCTGTG GACTCTCAGA TTCTTTACTA TGCAATGTTG 
GGGGTGAATC CCCGCTTTGA CTCAGCTTCC TCCAGCTACT ATTTGGACAT GCACAGCCTC 
CCCCATGTCA TCAACCCAGT GGAGTCCCGG CTGGGATCCA GTGCTGCCTC CTTGTACCCT 
GTGCTCAACT TTCTACTCTA CGTGCCTGAG CTTGCACACT CACCGCTGTA CATTCAGGAC 
AAGGATGGCG CTCCAGTGGC CACCAATGCC TTCCATAGTC CCCGCTGGGG TGGCATTATG 
GTATATAATG TTGACTCCAA AACCTATAAT GCCTCAGTGC TGCCAGTGAG AGTCGAGGTG 
GACATGGTGC GAGTGATGGA GGTGTTCCTG GCACAGTTGC GGTTGCTCTT TGGGATTGCT 
CAGCCCCAGC TGCCTCCAAA ATGCCTGCTT TCAGGGCCTA CGAGTGAAGG GCTAATGACC 
TGGGAGCTAG ACCGGCTGCT CTGGGCTCGG TCAGTGGAGA ACCTGGCCAC AGCCACCACC 
ACCCTTACCT CCCTGGCGCA GCTTCTGGGC AAGATCAGCA ACATTGTCAT TAAGGACGAC 
GTGGCATCTG AGGTGTACAA GGCTGTAGCT GCCGTCCAGA AGTCGGCAGA AGAGTTGGCG 
TCTGGGCACC TGGCATCTGC CTTTGTCGCC AGCCAGGAAG CTGTGACATC CTCTGAGCTT 
GCCTTCTTTG ACCCGTCACT CCTCCACCTC CTTTATTTCC CTGATGACCA GAAGTTTGCC 
ATCTACATCC CACTCTTCCT GCCTATGGCT GTGCCCATCC TCCTGTCCCT GGTCAAGATC 
TTCCTGGAGA CCCGCAAGTC CTGGAGAAAG CCTGAGAAGA CAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149975.1
CDS	374..2041
Translation	MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRKPEKTD

Target ORF information:

RefSeq Version	NM_033198.3
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class S (PIGS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033198.3

ORF Insert Sequence:

ATGGCGGCCG CCGGGGCTGC GGCTACACAC CTAGAGGTGG CCCGGGGCAA GCGCGCCGCC 
CTCTTCTTCG CTGCGGTGGC CATCGTGCTG GGGCTACCGC TCTGGTGGAA GACCACGGAG 
ACCTACCGGG CCTCGTTGCC TTACTCCCAG ATCAGTGGCC TGAATGCCCT TCAGCTCCGC 
CTCATGGTGC CTGTCACTGT CGTGTTTACG CGGGAGTCAG TGCCCCTGGA CGACCAGGAG 
AAGCTGCCCT TCACCGTTGT GCATGAAAGA GAGATTCCTC TGAAATACAA AATGAAAATC 
AAATGCCGTT TCCAGAAGGC CTATCGGAGG GCTTTGGACC ATGAGGAGGA GGCCCTGTCA 
TCGGGCAGTG TGCAAGAGGC AGAAGCCATG TTAGATGAGC CTCAGGAACA AGCGGAGGGC 
TCCCTGACTG TGTACGTGAT ATCTGAACAC TCCTCACTTC TTCCCCAGGA CATGATGAGC 
TACATTGGGC CCAAGAGGAC AGCAGTGGTG CGGGGGATAA TGCACCGGGA GGCCTTTAAC 
ATCATTGGCC GCCGCATAGT CCAGGTGGCC CAGGCCATGT CTTTGACTGA GGATGTGCTT 
GCTGCTGCTC TGGCTGACCA CCTTCCAGAG GACAAGTGGA GCGCTGAGAA GAGGCGGCCT 
CTCAAGTCCA GCTTGGGCTA TGAGATCACC TTCAGTTTAC TCAACCCAGA CCCCAAGTCC 
CATGATGTCT ACTGGGACAT TGAGGGGGCT GTCCGGCGCT ATGTGCAACC TTTCCTGAAT 
GCCCTCGGTG CCGCTGGCAA CTTCTCTGTG GACTCTCAGA TTCTTTACTA TGCAATGTTG 
GGGGTGAATC CCCGCTTTGA CTCAGCTTCC TCCAGCTACT ATTTGGACAT GCACAGCCTC 
CCCCATGTCA TCAACCCAGT GGAGTCCCGG CTGGGATCCA GTGCTGCCTC CTTGTACCCT 
GTGCTCAACT TTCTACTCTA CGTGCCTGAG CTTGCACACT CACCGCTGTA CATTCAGGAC 
AAGGATGGCG CTCCAGTGGC CACCAATGCC TTCCATAGTC CCCGCTGGGG TGGCATTATG 
GTATATAATG TTGACTCCAA AACCTATAAT GCCTCAGTGC TGCCAGTGAG AGTCGAGGTG 
GACATGGTGC GAGTGATGGA GGTGTTCCTG GCACAGTTGC GGTTGCTCTT TGGGATTGCT 
CAGCCCCAGC TGCCTCCAAA ATGCCTGCTT TCAGGGCCTA CGAGTGAAGG GCTAATGACC 
TGGGAGCTAG ACCGGCTGCT CTGGGCTCGG TCAGTGGAGA ACCTGGCCAC AGCCACCACC 
ACCCTTACCT CCCTGGCGCA GCTTCTGGGC AAGATCAGCA ACATTGTCAT TAAGGACGAC 
GTGGCATCTG AGGTGTACAA GGCTGTAGCT GCCGTCCAGA AGTCGGCAGA AGAGTTGGCG 
TCTGGGCACC TGGCATCTGC CTTTGTCGCC AGCCAGGAAG CTGTGACATC CTCTGAGCTT 
GCCTTCTTTG ACCCGTCACT CCTCCACCTC CTTTATTTCC CTGATGACCA GAAGTTTGCC 
ATCTACATCC CACTCTTCCT GCCTATGGCT GTGCCCATCC TCCTGTCCCT GGTCAAGATC 
TTCCTGGAGA CCCGCAAGTC CTGGAGAAAG CCTGAGAAGA CAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIGS ( NM_033198.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_149975.1 Homo sapiens phosphatidylinositol glycan anchor biosynthesis class S (PIGS), mRNA.

Price & Availability↑

CloneID	OHu05365
Clone ID Related Accession (Same CDS sequence)	NM_033198.3 , NM_033198.4
Accession Version	NM_033198.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Homo sapiens(human)
Product	GPI transamidase component PIG-S
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK075428.1, BC007301.1, AW084841.1 and AC005726.1. On Nov 22, 2018 this sequence version replaced NM_033198.3. ##Evidence-Data-START## Transcript exon combination :: BC069228.1, SRR3476690.865340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000308360.8/ ENSP00000309430.7 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCG CCGGGGCTGC GGCTACACAC CTAGAGGTGG CCCGGGGCAA GCGCGCCGCC 
CTCTTCTTCG CTGCGGTGGC CATCGTGCTG GGGCTACCGC TCTGGTGGAA GACCACGGAG 
ACCTACCGGG CCTCGTTGCC TTACTCCCAG ATCAGTGGCC TGAATGCCCT TCAGCTCCGC 
CTCATGGTGC CTGTCACTGT CGTGTTTACG CGGGAGTCAG TGCCCCTGGA CGACCAGGAG 
AAGCTGCCCT TCACCGTTGT GCATGAAAGA GAGATTCCTC TGAAATACAA AATGAAAATC 
AAATGCCGTT TCCAGAAGGC CTATCGGAGG GCTTTGGACC ATGAGGAGGA GGCCCTGTCA 
TCGGGCAGTG TGCAAGAGGC AGAAGCCATG TTAGATGAGC CTCAGGAACA AGCGGAGGGC 
TCCCTGACTG TGTACGTGAT ATCTGAACAC TCCTCACTTC TTCCCCAGGA CATGATGAGC 
TACATTGGGC CCAAGAGGAC AGCAGTGGTG CGGGGGATAA TGCACCGGGA GGCCTTTAAC 
ATCATTGGCC GCCGCATAGT CCAGGTGGCC CAGGCCATGT CTTTGACTGA GGATGTGCTT 
GCTGCTGCTC TGGCTGACCA CCTTCCAGAG GACAAGTGGA GCGCTGAGAA GAGGCGGCCT 
CTCAAGTCCA GCTTGGGCTA TGAGATCACC TTCAGTTTAC TCAACCCAGA CCCCAAGTCC 
CATGATGTCT ACTGGGACAT TGAGGGGGCT GTCCGGCGCT ATGTGCAACC TTTCCTGAAT 
GCCCTCGGTG CCGCTGGCAA CTTCTCTGTG GACTCTCAGA TTCTTTACTA TGCAATGTTG 
GGGGTGAATC CCCGCTTTGA CTCAGCTTCC TCCAGCTACT ATTTGGACAT GCACAGCCTC 
CCCCATGTCA TCAACCCAGT GGAGTCCCGG CTGGGATCCA GTGCTGCCTC CTTGTACCCT 
GTGCTCAACT TTCTACTCTA CGTGCCTGAG CTTGCACACT CACCGCTGTA CATTCAGGAC 
AAGGATGGCG CTCCAGTGGC CACCAATGCC TTCCATAGTC CCCGCTGGGG TGGCATTATG 
GTATATAATG TTGACTCCAA AACCTATAAT GCCTCAGTGC TGCCAGTGAG AGTCGAGGTG 
GACATGGTGC GAGTGATGGA GGTGTTCCTG GCACAGTTGC GGTTGCTCTT TGGGATTGCT 
CAGCCCCAGC TGCCTCCAAA ATGCCTGCTT TCAGGGCCTA CGAGTGAAGG GCTAATGACC 
TGGGAGCTAG ACCGGCTGCT CTGGGCTCGG TCAGTGGAGA ACCTGGCCAC AGCCACCACC 
ACCCTTACCT CCCTGGCGCA GCTTCTGGGC AAGATCAGCA ACATTGTCAT TAAGGACGAC 
GTGGCATCTG AGGTGTACAA GGCTGTAGCT GCCGTCCAGA AGTCGGCAGA AGAGTTGGCG 
TCTGGGCACC TGGCATCTGC CTTTGTCGCC AGCCAGGAAG CTGTGACATC CTCTGAGCTT 
GCCTTCTTTG ACCCGTCACT CCTCCACCTC CTTTATTTCC CTGATGACCA GAAGTTTGCC 
ATCTACATCC CACTCTTCCT GCCTATGGCT GTGCCCATCC TCCTGTCCCT GGTCAAGATC 
TTCCTGGAGA CCCGCAAGTC CTGGAGAAAG CCTGAGAAGA CAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_149975.1
CDS	29..1696
Translation	MAAAGAAATHLEVARGKRAALFFAAVAIVLGLPLWWKTTETYRASLPYSQISGLNALQLRLMVPVTVVFTRESVPLDDQEKLPFTVVHEREIPLKYKMKIKCRFQKAYRRALDHEEEALSSGSVQEAEAMLDEPQEQAEGSLTVYVISEHSSLLPQDMMSYIGPKRTAVVRGIMHREAFNIIGRRIVQVAQAMSLTEDVLAAALADHLPEDKWSAEKRRPLKSSLGYEITFSLLNPDPKSHDVYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAMLGVNPRFDSASSSYYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYIQDKDGAPVATNAFHSPRWGGIMVYNVDSKTYNASVLPVRVEVDMVRVMEVFLAQLRLLFGIAQPQLPPKCLLSGPTSEGLMTWELDRLLWARSVENLATATTTLTSLAQLLGKISNIVIKDDVASEVYKAVAAVQKSAEELASGHLASAFVASQEAVTSSELAFFDPSLLHLLYFPDDQKFAIYIPLFLPMAVPILLSLVKIFLETRKSWRKPEKTD

Target ORF information:

RefSeq Version	NM_033198.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphatidylinositol glycan anchor biosynthesis class S (PIGS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033198.4

ORF Insert Sequence:

ATGGCGGCCG CCGGGGCTGC GGCTACACAC CTAGAGGTGG CCCGGGGCAA GCGCGCCGCC 
CTCTTCTTCG CTGCGGTGGC CATCGTGCTG GGGCTACCGC TCTGGTGGAA GACCACGGAG 
ACCTACCGGG CCTCGTTGCC TTACTCCCAG ATCAGTGGCC TGAATGCCCT TCAGCTCCGC 
CTCATGGTGC CTGTCACTGT CGTGTTTACG CGGGAGTCAG TGCCCCTGGA CGACCAGGAG 
AAGCTGCCCT TCACCGTTGT GCATGAAAGA GAGATTCCTC TGAAATACAA AATGAAAATC 
AAATGCCGTT TCCAGAAGGC CTATCGGAGG GCTTTGGACC ATGAGGAGGA GGCCCTGTCA 
TCGGGCAGTG TGCAAGAGGC AGAAGCCATG TTAGATGAGC CTCAGGAACA AGCGGAGGGC 
TCCCTGACTG TGTACGTGAT ATCTGAACAC TCCTCACTTC TTCCCCAGGA CATGATGAGC 
TACATTGGGC CCAAGAGGAC AGCAGTGGTG CGGGGGATAA TGCACCGGGA GGCCTTTAAC 
ATCATTGGCC GCCGCATAGT CCAGGTGGCC CAGGCCATGT CTTTGACTGA GGATGTGCTT 
GCTGCTGCTC TGGCTGACCA CCTTCCAGAG GACAAGTGGA GCGCTGAGAA GAGGCGGCCT 
CTCAAGTCCA GCTTGGGCTA TGAGATCACC TTCAGTTTAC TCAACCCAGA CCCCAAGTCC 
CATGATGTCT ACTGGGACAT TGAGGGGGCT GTCCGGCGCT ATGTGCAACC TTTCCTGAAT 
GCCCTCGGTG CCGCTGGCAA CTTCTCTGTG GACTCTCAGA TTCTTTACTA TGCAATGTTG 
GGGGTGAATC CCCGCTTTGA CTCAGCTTCC TCCAGCTACT ATTTGGACAT GCACAGCCTC 
CCCCATGTCA TCAACCCAGT GGAGTCCCGG CTGGGATCCA GTGCTGCCTC CTTGTACCCT 
GTGCTCAACT TTCTACTCTA CGTGCCTGAG CTTGCACACT CACCGCTGTA CATTCAGGAC 
AAGGATGGCG CTCCAGTGGC CACCAATGCC TTCCATAGTC CCCGCTGGGG TGGCATTATG 
GTATATAATG TTGACTCCAA AACCTATAAT GCCTCAGTGC TGCCAGTGAG AGTCGAGGTG 
GACATGGTGC GAGTGATGGA GGTGTTCCTG GCACAGTTGC GGTTGCTCTT TGGGATTGCT 
CAGCCCCAGC TGCCTCCAAA ATGCCTGCTT TCAGGGCCTA CGAGTGAAGG GCTAATGACC 
TGGGAGCTAG ACCGGCTGCT CTGGGCTCGG TCAGTGGAGA ACCTGGCCAC AGCCACCACC 
ACCCTTACCT CCCTGGCGCA GCTTCTGGGC AAGATCAGCA ACATTGTCAT TAAGGACGAC 
GTGGCATCTG AGGTGTACAA GGCTGTAGCT GCCGTCCAGA AGTCGGCAGA AGAGTTGGCG 
TCTGGGCACC TGGCATCTGC CTTTGTCGCC AGCCAGGAAG CTGTGACATC CTCTGAGCTT 
GCCTTCTTTG ACCCGTCACT CCTCCACCTC CTTTATTTCC CTGATGACCA GAAGTTTGCC 
ATCTACATCC CACTCTTCCT GCCTATGGCT GTGCCCATCC TCCTGTCCCT GGTCAAGATC 
TTCCTGGAGA CCCGCAAGTC CTGGAGAAAG CCTGAGAAGA CAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

Endoplasmic reticulum localization of Gaa1 and PIG-T, subunits of the glycosylphosphatidylinositol transamidase complex.
The Journal of biological chemistry280(16)16402-9(2005 Apr)
Vainauskas S,Menon AK

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

Human PIG-U and yeast Cdc91p are the fifth subunit of GPI transamidase that attaches GPI-anchors to proteins.
Molecular biology of the cell14(5)1780-9(2003 May)
Hong Y,Ohishi K,Kang JY,Tanaka S,Inoue N,Nishimura J,Maeda Y,Kinoshita T

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PIGS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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