CIAO1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CIAO1
Entrez Gene ID	9391
Full Name	cytosolic iron-sulfur assembly component 1
Synonyms	CIA1,WDR39
General protein information	Preferred Names cytosolic iron-sulfur assembly component 1 Names probable cytosolic iron-sulfur protein assembly protein CIAO1 WD repeat domain 39 WD repeat-containing protein 39 WD40 protein Ciao1 cytosolic iron-sulfur protein assembly 1 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q11.2
Disorder MIM:	604333

mRNA and Protein(s)

mRNA	Protein	Name
NM_004804.2	NP_004795.1	probable cytosolic iron-sulfur protein assembly protein CIAO1
NM_004804.3	NP_004795.1	probable cytosolic iron-sulfur protein assembly protein CIAO1
XM_024453247.1	XP_024309015.1	probable cytosolic iron-sulfur protein assembly protein CIAO1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CIAO1	NP_004795.1
	CIAO1	XP_001098395.1
Bos taurus (cattle)	CIAO1	NP_001032559.1
Kluyveromyces lactis	KLLA0E21781g	XP_454937.1
Eremothecium gossypii	AGOS_ACR091W	NP_983493.1
Danio rerio (zebrafish)	ciao1	NP_956441.2
Drosophila melanogaster (fruit fly)	Ciao1	NP_610996.1
Anopheles gambiae (African malaria mosquito)	CIAO1_ANOGA	XP_310648.3
Saccharomyces cerevisiae (baker's yeast)	CIA1	NP_010553.3
Neurospora crassa	NCU05426	XP_963249.1
Xenopus tropicalis (tropical clawed frog)	ciao1	NP_001016887.1
Canis lupus familiaris (dog)	CIAO1	XP_532950.2
Arabidopsis thaliana (thale cress)	AT4G32990	NP_195025.5
Mus musculus (house mouse)	Ciao1	NP_079572.2
Rattus norvegicus (Norway rat)	Ciao1	NP_001008766.1
Oryza sativa (rice)	Os07g0252000	NP_001059302.1
Pan troglodytes (chimpanzee)	CIAO1	XP_001144741.2
Gallus gallus (chicken)	CIAO1	XP_423992.4
Caenorhabditis elegans (roundworm)	CELE_Y18D10A.9	NP_493247.2
Magnaporthe oryzae (rice blast fungus)	MGG_03150	XP_003716845.1
Arabidopsis thaliana (thale cress)	emb1345	NP_565615.1

Gene Ontology
Bibliography

Related articles in PubMed

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.
Kim KS, Maio N, Singh A, Rouault TA
Human molecular genetics(2018 Jan)

Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.
Upadhyay AS, Stehling O, Panayiotou C, R?sser R, Lill R, ?verby AK
The Journal of biological chemistry292(33)13879-13889(2017 08)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.
Odermatt DC, Gari K
Cell reports18(6)1434-1443(2017 Feb)

Human DNA polymerase ? is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.
Moiseeva TN, Gamper AM, Hood BL, Conrads TP, Bakkenist CJ
DNA repair439-17(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that CIA2B and MMS19 physically interact with C-terminus of viperin/RSAD2; CIAO1 appears to function as primary viperin-interacting protein; CIA2A binds to N-terminus of viperin in CIAO1-, CIA2B-, and MMS19-independent fashion. (CIA2B = metallochaperone CIA2B/FAM96B; MMS19 = transcription factor MMS19; CIAO1 = cytosolic iron-sulfur assembly component 1; CIA2A = metallochaperone CIA2A/Fam96a)
Title: Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.

POLE1 is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.
Title: Human DNA polymerase ? is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.

These data suggest that viperin requires CIAO1 for [4Fe-4S] cluster assembly, and acts through an enzymatic, Fe-S cluster- and SAM-dependent mechanism to inhibit viral RNA synthesis.
Title: Viperin is an iron-sulfur protein that inhibits genome synthesis of tick-borne encephalitis virus via radical SAM domain activity.

CIA2B-CIA1-MMS19 and CIA2A-CIA1 assist different branches of Fe/S protein assembly and intimately link this process to cellular iron regulation via IRP1 Fe/S cluster maturation and IRP2 stabilization.
Title: Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins.

The following CIAO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CIAO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15168	NM_004804.3 Latest version!	Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu105008	XM_024453247.1 Latest version!	Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu15168	NM_004804.2	Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CIAO1 ( NM_004804.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004795.1 Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), mRNA.

Price & Availability↑

CloneID	OHu15168
Clone ID Related Accession (Same CDS sequence)	NM_004804.2 , NM_004804.3
Accession Version	NM_004804.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	probable cytosolic iron-sulfur protein assembly protein CIAO1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI460314.1, BC032812.1, AK124703.1, BE220199.1, BG108391.1 and AI094333.1. On Dec 1, 2003 this sequence version replaced NM_004804.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.4632.1, SRR1803613.194897.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGACT CGCTGGTGCT GCTGGGCCGT GTCCCGGCGC ACCCGGACTC CCGCTGCTGG 
TTCCTGGCCT GGAACCCCGC GGGGACCCTG CTGGCCTCGT GCGGCGGCGA CCGGAGAATC 
CGCATCTGGG GCACGGAGGG TGACAGCTGG ATCTGCAAGT CTGTCCTTTC TGAAGGCCAC 
CAGCGCACCG TGCGGAAGGT AGCCTGGTCC CCCTGCGGTA ATTACCTGGC CTCTGCCAGC 
TTTGATGCTA CCACTTGCAT TTGGAAGAAG AACCAGGATG ACTTTGAGTG TGTAACCACT 
CTCGAGGGCC ATGAAAATGA GGTCAAGTCA GTGGCTTGGG CCCCATCTGG CAACCTCCTG 
GCCACTTGCA GCCGAGATAA GAGCGTTTGG GTCTGGGAAG TTGATGAAGA GGATGAGTAT 
GAATGTGTCA GTGTTCTCAA CTCCCACACA CAGGATGTCA AGCATGTGGT TTGGCACCCA 
AGTCAGGAGC TCTTAGCTTC TGCCAGCTAT GATGACACAG TGAAGCTGTA CCGGGAGGAA 
GAGGATGACT GGGTATGCTG TGCCACCCTT GAGGGCCATG AATCCACTGT GTGGAGCTTG 
GCCTTTGACC CGAGTGGCCA GCGCCTGGCG TCTTGTAGTG ATGACCGTAC TGTGCGTATC 
TGGCGTCAGT ATCTACCAGG CAATGAACAA GGGGTGGCAT GCAGCGGCTC TGACCCCAGT 
TGGAAATGTA TCTGTACTTT GTCCGGCTTC CACTCAAGGA CCATTTATGA CATTGCTTGG 
TGTCAGCTGA CAGGGGCTCT GGCCACAGCT TGTGGGGATG ACGCGATCCG CGTGTTTCAG 
GAGGATCCCA ACTCGGATCC ACAGCAGCCC ACCTTCTCCC TGACAGCCCA CTTGCATCAG 
GCCCATTCCC AGGATGTCAA CTGTGTGGCC TGGAACCCCA AGGAGCCAGG GCTACTGGCC 
TCCTGCAGTG ATGATGGGGA GGTGGCCTTC TGGAAGTATC AGCGGCCTGA AGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004795.1
CDS	206..1225
Translation	MKDSLVLLGRVPAHPDSRCWFLAWNPAGTLLASCGGDRRIRIWGTEGDSWICKSVLSEGHQRTVRKVAWSPCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEVDEEDEYECVSVLNSHTQDVKHVVWHPSQELLASASYDDTVKLYREEEDDWVCCATLEGHESTVWSLAFDPSGQRLASCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQEDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQRPEGL

Target ORF information:

RefSeq Version	NM_004804.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004804.2

ORF Insert Sequence:

ATGAAGGACT CGCTGGTGCT GCTGGGCCGT GTCCCGGCGC ACCCGGACTC CCGCTGCTGG 
TTCCTGGCCT GGAACCCCGC GGGGACCCTG CTGGCCTCGT GCGGCGGCGA CCGGAGAATC 
CGCATCTGGG GCACGGAGGG TGACAGCTGG ATCTGCAAGT CTGTCCTTTC TGAAGGCCAC 
CAGCGCACCG TGCGGAAGGT AGCCTGGTCC CCCTGCGGTA ATTACCTGGC CTCTGCCAGC 
TTTGATGCTA CCACTTGCAT TTGGAAGAAG AACCAGGATG ACTTTGAGTG TGTAACCACT 
CTCGAGGGCC ATGAAAATGA GGTCAAGTCA GTGGCTTGGG CCCCATCTGG CAACCTCCTG 
GCCACTTGCA GCCGAGATAA GAGCGTTTGG GTCTGGGAAG TTGATGAAGA GGATGAGTAT 
GAATGTGTCA GTGTTCTCAA CTCCCACACA CAGGATGTCA AGCATGTGGT TTGGCACCCA 
AGTCAGGAGC TCTTAGCTTC TGCCAGCTAT GATGACACAG TGAAGCTGTA CCGGGAGGAA 
GAGGATGACT GGGTATGCTG TGCCACCCTT GAGGGCCATG AATCCACTGT GTGGAGCTTG 
GCCTTTGACC CGAGTGGCCA GCGCCTGGCG TCTTGTAGTG ATGACCGTAC TGTGCGTATC 
TGGCGTCAGT ATCTACCAGG CAATGAACAA GGGGTGGCAT GCAGCGGCTC TGACCCCAGT 
TGGAAATGTA TCTGTACTTT GTCCGGCTTC CACTCAAGGA CCATTTATGA CATTGCTTGG 
TGTCAGCTGA CAGGGGCTCT GGCCACAGCT TGTGGGGATG ACGCGATCCG CGTGTTTCAG 
GAGGATCCCA ACTCGGATCC ACAGCAGCCC ACCTTCTCCC TGACAGCCCA CTTGCATCAG 
GCCCATTCCC AGGATGTCAA CTGTGTGGCC TGGAACCCCA AGGAGCCAGG GCTACTGGCC 
TCCTGCAGTG ATGATGGGGA GGTGGCCTTC TGGAAGTATC AGCGGCCTGA AGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIAO1 ( NM_004804.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004795.1 Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), mRNA.

Price & Availability↑

CloneID	OHu15168
Clone ID Related Accession (Same CDS sequence)	NM_004804.2 , NM_004804.3
Accession Version	NM_004804.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	probable cytosolic iron-sulfur protein assembly protein CIAO1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI460314.1, BC032812.1, AK124703.1, BE220199.1, BG108391.1 and AI094333.1. On May 17, 2019 this sequence version replaced NM_004804.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.4632.1, SRR1803611.196354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000488633.2/ ENSP00000418287.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGGACT CGCTGGTGCT GCTGGGCCGT GTCCCGGCGC ACCCGGACTC CCGCTGCTGG 
TTCCTGGCCT GGAACCCCGC GGGGACCCTG CTGGCCTCGT GCGGCGGCGA CCGGAGAATC 
CGCATCTGGG GCACGGAGGG TGACAGCTGG ATCTGCAAGT CTGTCCTTTC TGAAGGCCAC 
CAGCGCACCG TGCGGAAGGT AGCCTGGTCC CCCTGCGGTA ATTACCTGGC CTCTGCCAGC 
TTTGATGCTA CCACTTGCAT TTGGAAGAAG AACCAGGATG ACTTTGAGTG TGTAACCACT 
CTCGAGGGCC ATGAAAATGA GGTCAAGTCA GTGGCTTGGG CCCCATCTGG CAACCTCCTG 
GCCACTTGCA GCCGAGATAA GAGCGTTTGG GTCTGGGAAG TTGATGAAGA GGATGAGTAT 
GAATGTGTCA GTGTTCTCAA CTCCCACACA CAGGATGTCA AGCATGTGGT TTGGCACCCA 
AGTCAGGAGC TCTTAGCTTC TGCCAGCTAT GATGACACAG TGAAGCTGTA CCGGGAGGAA 
GAGGATGACT GGGTATGCTG TGCCACCCTT GAGGGCCATG AATCCACTGT GTGGAGCTTG 
GCCTTTGACC CGAGTGGCCA GCGCCTGGCG TCTTGTAGTG ATGACCGTAC TGTGCGTATC 
TGGCGTCAGT ATCTACCAGG CAATGAACAA GGGGTGGCAT GCAGCGGCTC TGACCCCAGT 
TGGAAATGTA TCTGTACTTT GTCCGGCTTC CACTCAAGGA CCATTTATGA CATTGCTTGG 
TGTCAGCTGA CAGGGGCTCT GGCCACAGCT TGTGGGGATG ACGCGATCCG CGTGTTTCAG 
GAGGATCCCA ACTCGGATCC ACAGCAGCCC ACCTTCTCCC TGACAGCCCA CTTGCATCAG 
GCCCATTCCC AGGATGTCAA CTGTGTGGCC TGGAACCCCA AGGAGCCAGG GCTACTGGCC 
TCCTGCAGTG ATGATGGGGA GGTGGCCTTC TGGAAGTATC AGCGGCCTGA AGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004795.1
CDS	127..1146
Translation	MKDSLVLLGRVPAHPDSRCWFLAWNPAGTLLASCGGDRRIRIWGTEGDSWICKSVLSEGHQRTVRKVAWSPCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEVDEEDEYECVSVLNSHTQDVKHVVWHPSQELLASASYDDTVKLYREEEDDWVCCATLEGHESTVWSLAFDPSGQRLASCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQEDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQRPEGL

Target ORF information:

RefSeq Version	NM_004804.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004804.3

ORF Insert Sequence:

ATGAAGGACT CGCTGGTGCT GCTGGGCCGT GTCCCGGCGC ACCCGGACTC CCGCTGCTGG 
TTCCTGGCCT GGAACCCCGC GGGGACCCTG CTGGCCTCGT GCGGCGGCGA CCGGAGAATC 
CGCATCTGGG GCACGGAGGG TGACAGCTGG ATCTGCAAGT CTGTCCTTTC TGAAGGCCAC 
CAGCGCACCG TGCGGAAGGT AGCCTGGTCC CCCTGCGGTA ATTACCTGGC CTCTGCCAGC 
TTTGATGCTA CCACTTGCAT TTGGAAGAAG AACCAGGATG ACTTTGAGTG TGTAACCACT 
CTCGAGGGCC ATGAAAATGA GGTCAAGTCA GTGGCTTGGG CCCCATCTGG CAACCTCCTG 
GCCACTTGCA GCCGAGATAA GAGCGTTTGG GTCTGGGAAG TTGATGAAGA GGATGAGTAT 
GAATGTGTCA GTGTTCTCAA CTCCCACACA CAGGATGTCA AGCATGTGGT TTGGCACCCA 
AGTCAGGAGC TCTTAGCTTC TGCCAGCTAT GATGACACAG TGAAGCTGTA CCGGGAGGAA 
GAGGATGACT GGGTATGCTG TGCCACCCTT GAGGGCCATG AATCCACTGT GTGGAGCTTG 
GCCTTTGACC CGAGTGGCCA GCGCCTGGCG TCTTGTAGTG ATGACCGTAC TGTGCGTATC 
TGGCGTCAGT ATCTACCAGG CAATGAACAA GGGGTGGCAT GCAGCGGCTC TGACCCCAGT 
TGGAAATGTA TCTGTACTTT GTCCGGCTTC CACTCAAGGA CCATTTATGA CATTGCTTGG 
TGTCAGCTGA CAGGGGCTCT GGCCACAGCT TGTGGGGATG ACGCGATCCG CGTGTTTCAG 
GAGGATCCCA ACTCGGATCC ACAGCAGCCC ACCTTCTCCC TGACAGCCCA CTTGCATCAG 
GCCCATTCCC AGGATGTCAA CTGTGTGGCC TGGAACCCCA AGGAGCCAGG GCTACTGGCC 
TCCTGCAGTG ATGATGGGGA GGTGGCCTTC TGGAAGTATC AGCGGCCTGA AGGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CIAO1 ( XM_024453247.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309015.1 Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu105008
Clone ID Related Accession (Same CDS sequence)	XM_024453247.1
Accession Version	XM_024453247.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable cytosolic iron-sulfur protein assembly protein CIAO1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGACT CGCTGGTGCT GCTGGGCCGT GTCCCGGCGC ACCCGGACTC CCGCTGCTGG 
TTCCTGGCCT GGAACCCCGC GGGGACCCTG CTGGCCTCGT GCGGCGGCGA CCGGAGAATC 
CGCATCTGGG GCACGGAGGG TGACAGCTGG ATCTGCAAGT CTGTCCTTTC TGAAGGCCAC 
CAGCGCACCG TGCGGAAGGT AGCCTGGTCC CCCTGCGGTA ATTACCTGGC CTCTGCCAGC 
TTTGATGCTA CCACTTGCAT TTGGAAGAAG AACCAGGATG ACTTTGAGTG TGTAACCACT 
CTCGAGGGCC ATGAAAATGA GGTCAAGTCA GTGGCTTGGG CCCCATCTGG CAACCTCCTG 
GCCACTTGCA GCCGAGATAA GAGCGTTTGG GTCTGGGAAG GTGAGGCCAG GTCCCTCCAG 
GTGGATTGGG AACCACCTGA CAGCCCCCTC TGTGTCCCTG ACAGGGTCGG CTCTTGGGTC 
CCCTTTTTCT CTCCCACAGT TGATGAAGAG GATGAGTATG AATGTGTCAG TGTTCTCAAC 
TCCCACACAC AGGATGTCAA GCATGTGGTT TGGCACCCAA GTCAGGAGCT CTTAGCTTCT 
GCCAGCTATG ATGACACAGT GAAGCTGTAC CGGGAGGAAG AGGATGACTG GGTATGCTGT 
GCCACCCTTG AGGGCCATGA ATCCACTGTG TGGAGCTTGG CCTTTGACCC GAGTGGCCAG 
CGCCTGGCGT CTTGTAGTGA TGACCGTACT GTGCGTATCT GGCGTCAGTA TCTACCAGGC 
AATGAACAAG GGGTGGCATG CAGCGGCTCT GACCCCAGTT GGAAATGTAT CTGTACTTTG 
TCCGGCTTCC ACTCAAGGAC CATTTATGAC ATTGCTTGGT GTCAGCTGAC AGGGGCTCTG 
GCCACAGCTT GTGGGGATGA CGCGATCCGC GTGTTTCAGG AGGATCCCAA CTCGGATCCA 
CAGCAGCCCA CCTTCTCCCT GACAGCCCAC TTGCATCAGG CCCATTCCCA GGATGTCAAC 
TGTGTGGCCT GGAACCCCAA GGAGCCAGGG CTACTGGCCT CCTGCAGTGA TGATGGGGAG 
GTGGCCTTCT GGAAGTATCA GCGGCCTGAA GGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309015.1
CDS	193..1311
Translation	MKDSLVLLGRVPAHPDSRCWFLAWNPAGTLLASCGGDRRIRIWGTEGDSWICKSVLSEGHQRTVRKVAWSPCGNYLASASFDATTCIWKKNQDDFECVTTLEGHENEVKSVAWAPSGNLLATCSRDKSVWVWEGEARSLQVDWEPPDSPLCVPDRVGSWVPFFSPTVDEEDEYECVSVLNSHTQDVKHVVWHPSQELLASASYDDTVKLYREEEDDWVCCATLEGHESTVWSLAFDPSGQRLASCSDDRTVRIWRQYLPGNEQGVACSGSDPSWKCICTLSGFHSRTIYDIAWCQLTGALATACGDDAIRVFQEDPNSDPQQPTFSLTAHLHQAHSQDVNCVAWNPKEPGLLASCSDDGEVAFWKYQRPEGL

Target ORF information:

RefSeq Version	XM_024453247.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cytosolic iron-sulfur assembly component 1 (CIAO1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453247.1

ORF Insert Sequence:

ATGAAGGACT CGCTGGTGCT GCTGGGCCGT GTCCCGGCGC ACCCGGACTC CCGCTGCTGG 
TTCCTGGCCT GGAACCCCGC GGGGACCCTG CTGGCCTCGT GCGGCGGCGA CCGGAGAATC 
CGCATCTGGG GCACGGAGGG TGACAGCTGG ATCTGCAAGT CTGTCCTTTC TGAAGGCCAC 
CAGCGCACCG TGCGGAAGGT AGCCTGGTCC CCCTGCGGTA ATTACCTGGC CTCTGCCAGC 
TTTGATGCTA CCACTTGCAT TTGGAAGAAG AACCAGGATG ACTTTGAGTG TGTAACCACT 
CTCGAGGGCC ATGAAAATGA GGTCAAGTCA GTGGCTTGGG CCCCATCTGG CAACCTCCTG 
GCCACTTGCA GCCGAGATAA GAGCGTTTGG GTCTGGGAAG GTGAGGCCAG GTCCCTCCAG 
GTGGATTGGG AACCACCTGA CAGCCCCCTC TGTGTCCCTG ACAGGGTCGG CTCTTGGGTC 
CCCTTTTTCT CTCCCACAGT TGATGAAGAG GATGAGTATG AATGTGTCAG TGTTCTCAAC 
TCCCACACAC AGGATGTCAA GCATGTGGTT TGGCACCCAA GTCAGGAGCT CTTAGCTTCT 
GCCAGCTATG ATGACACAGT GAAGCTGTAC CGGGAGGAAG AGGATGACTG GGTATGCTGT 
GCCACCCTTG AGGGCCATGA ATCCACTGTG TGGAGCTTGG CCTTTGACCC GAGTGGCCAG 
CGCCTGGCGT CTTGTAGTGA TGACCGTACT GTGCGTATCT GGCGTCAGTA TCTACCAGGC 
AATGAACAAG GGGTGGCATG CAGCGGCTCT GACCCCAGTT GGAAATGTAT CTGTACTTTG 
TCCGGCTTCC ACTCAAGGAC CATTTATGAC ATTGCTTGGT GTCAGCTGAC AGGGGCTCTG 
GCCACAGCTT GTGGGGATGA CGCGATCCGC GTGTTTCAGG AGGATCCCAA CTCGGATCCA 
CAGCAGCCCA CCTTCTCCCT GACAGCCCAC TTGCATCAGG CCCATTCCCA GGATGTCAAC 
TGTGTGGCCT GGAACCCCAA GGAGCCAGGG CTACTGGCCT CCTGCAGTGA TGATGGGGAG 
GTGGCCTTCT GGAAGTATCA GCGGCCTGAA GGCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cytosolic HSC20 integrates de novo iron-sulfur cluster biogenesis with the CIAO1-mediated transfer to recipients.
Human molecular genetics(2018 Jan)
Kim KS,Maio N,Singh A,Rouault TA

Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.
The Journal of biological chemistry292(33)13879-13889(2017 08)
Upadhyay AS,Stehling O,Panayiotou C,R?sser R,Lill R,?verby AK

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The CIA Targeting Complex Is Highly Regulated and Provides Two Distinct Binding Sites for Client Iron-Sulfur Proteins.
Cell reports18(6)1434-1443(2017 Feb)
Odermatt DC,Gari K

Human DNA polymerase ? is phosphorylated at serine-1940 after DNA damage and interacts with the iron-sulfur complex chaperones CIAO1 and MMS19.
DNA repair439-17(2016 07)
Moiseeva TN,Gamper AM,Hood BL,Conrads TP,Bakkenist CJ