KL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KL
Entrez Gene ID	9365
Full Name	klotho
General protein information	Preferred Names klotho Names klotho
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q13.1
Summary	This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008].
Disorder MIM:	604824
Disorder Html:	{Coronary artery disease, susceptibility to} (3); Tumoral calcinosis, hyperphosphatemic, 211900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006719895.2	XP_006719958.1	klotho isoform X1
NM_004795.3	NP_004786.2	klotho precursor
NM_004795.4	NP_004786.2	klotho precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	KL	XP_522655.2
	LOC714042	XP_001101127.1
Homo sapiens (human)	KL	NP_004786.2
Canis lupus familiaris (dog)	KL	XP_003433357.1
Bos taurus (cattle)	KL	NP_001178124.1
Mus musculus (house mouse)	Kl	NP_038851.2
Danio rerio (zebrafish)	kl	XP_690797.4
Rattus norvegicus (Norway rat)	Kl	NP_112626.1
Gallus gallus (chicken)	KL	XP_004938814.1
Xenopus tropicalis (tropical clawed frog)	kl	XP_002934067.1

Gene Ontology

The following KL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18278	NM_004795.4 Latest version!	Homo sapiens klotho (KL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu47313	XM_006719895.2 Latest version!	Homo sapiens klotho (KL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
View Old Accession Versions >>
OHu18278	NM_004795.3	Homo sapiens klotho (KL), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KL ( NM_004795.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004786.2 Homo sapiens klotho (KL), mRNA.

Price & Availability↑

CloneID	OHu18278
Clone ID Related Accession (Same CDS sequence)	NM_004795.3 , NM_004795.4
Accession Version	NM_004795.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	klotho precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161898.17, AB005142.1 and AA890465.1. This sequence is a reference standard in the RefSeqGene project. On Oct 16, 2008 this sequence version replaced NM_004795.2. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB005142.1, SRR1803611.233700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGCCA GCGCCCCGCC GCGCCGCCCG CGGCCGCCGC CGCCGTCGCT GTCGCTGCTG 
CTGGTGCTGC TGGGCCTGGG CGGCCGCCGC CTGCGTGCGG AGCCGGGCGA CGGCGCGCAG 
ACCTGGGCCC GTTTCTCGCG GCCTCCTGCC CCCGAGGCCG CGGGCCTCTT CCAGGGCACC 
TTCCCCGACG GCTTCCTCTG GGCCGTGGGC AGCGCCGCCT ACCAGACCGA GGGCGGCTGG 
CAGCAGCACG GCAAGGGTGC GTCCATCTGG GATACGTTCA CCCACCACCC CCTGGCACCC 
CCGGGAGACT CCCGGAACGC CAGTCTGCCG TTGGGCGCCC CGTCGCCGCT GCAGCCCGCC 
ACCGGGGACG TAGCCAGCGA CAGCTACAAC AACGTCTTCC GCGACACGGA GGCGCTGCGC 
GAGCTCGGGG TCACTCACTA CCGCTTCTCC ATCTCGTGGG CGCGAGTGCT CCCCAATGGC 
AGCGCGGGCG TCCCCAACCG CGAGGGGCTG CGCTACTACC GGCGCCTGCT GGAGCGGCTG 
CGGGAGCTGG GCGTGCAGCC CGTGGTCACC CTGTACCACT GGGACCTGCC CCAGCGCCTG 
CAGGACGCCT ACGGCGGCTG GGCCAACCGC GCCCTGGCCG ACCACTTCAG GGATTACGCG 
GAGCTCTGCT TCCGCCACTT CGGCGGTCAG GTCAAGTACT GGATCACCAT CGACAACCCC 
TACGTGGTGG CCTGGCACGG CTACGCCACC GGGCGCCTGG CCCCCGGCAT CCGGGGCAGC 
CCGCGGCTCG GGTACCTGGT GGCGCACAAC CTCCTCCTGG CTCATGCCAA AGTCTGGCAT 
CTCTACAATA CTTCTTTCCG TCCCACTCAG GGAGGTCAGG TGTCCATTGC CCTAAGCTCT 
CACTGGATCA ATCCTCGAAG AATGACCGAC CACAGCATCA AAGAATGTCA AAAATCTCTG 
GACTTTGTAC TAGGTTGGTT TGCCAAACCC GTATTTATTG ATGGTGACTA TCCCGAGAGC 
ATGAAGAATA ACCTTTCATC TATTCTGCCT GATTTTACTG AATCTGAGAA AAAGTTCATC 
AAAGGAACTG CTGACTTTTT TGCTCTTTGC TTTGGACCCA CCTTGAGTTT TCAACTTTTG 
GACCCTCACA TGAAGTTCCG CCAATTGGAA TCTCCCAACC TGAGGCAACT GCTTTCCTGG 
ATTGACCTTG AATTTAACCA TCCTCAAATA TTTATTGTGG AAAATGGCTG GTTTGTCTCA 
GGGACCACCA AGAGAGATGA TGCCAAATAT ATGTATTACC TCAAAAAGTT CATCATGGAA 
ACCTTAAAAG CCATCAAGCT GGATGGGGTG GATGTCATCG GGTATACCGC ATGGTCCCTC 
ATGGATGGTT TCGAGTGGCA CAGAGGTTAC AGCATCAGGC GTGGACTCTT CTATGTTGAC 
TTTCTAAGCC AGGACAAGAT GTTGTTGCCA AAGTCTTCAG CCTTGTTCTA CCAAAAGCTG 
ATAGAGAAAA ATGGCTTCCC TCCTTTACCT GAAAATCAGC CCCTAGAAGG GACATTTCCC 
TGTGACTTTG CTTGGGGAGT TGTTGACAAC TACATTCAAG TAGATACCAC TCTGTCTCAG 
TTTACCGACC TGAATGTTTA CCTGTGGGAT GTCCACCACA GTAAAAGGCT TATTAAAGTG 
GATGGGGTTG TGACCAAGAA GAGGAAATCC TACTGTGTTG ACTTTGCTGC CATCCAGCCC 
CAGATCGCTT TACTCCAGGA AATGCACGTT ACACATTTTC GCTTCTCCCT GGACTGGGCC 
CTGATTCTCC CTCTGGGTAA CCAGTCCCAG GTGAACCACA CCATCCTGCA GTACTATCGC 
TGCATGGCCA GCGAGCTTGT CCGTGTCAAC ATCACCCCAG TGGTGGCCCT GTGGCAGCCT 
ATGGCCCCGA ACCAAGGACT GCCGCGCCTC CTGGCCAGGC AGGGCGCCTG GGAGAACCCC 
TACACTGCCC TGGCCTTTGC AGAGTATGCC CGACTGTGCT TTCAAGAGCT CGGCCATCAC 
GTCAAGCTTT GGATAACGAT GAATGAGCCG TATACAAGGA ATATGACATA CAGTGCTGGC 
CACAACCTTC TGAAGGCCCA TGCCCTGGCT TGGCATGTGT ACAATGAAAA GTTTAGGCAT 
GCTCAGAATG GGAAAATATC CATAGCCTTG CAGGCTGATT GGATAGAACC TGCCTGCCCT 
TTCTCCCAAA AGGACAAAGA GGTGGCTGAG AGAGTTTTGG AATTTGACAT TGGCTGGCTG 
GCTGAGCCCA TTTTCGGCTC TGGAGATTAT CCATGGGTGA TGAGGGACTG GCTGAACCAA 
AGAAACAATT TTCTTCTTCC TTATTTCACT GAAGATGAAA AAAAGCTAAT CCAGGGTACC 
TTTGACTTTT TGGCTTTAAG CCATTATACC ACCATCCTTG TAGACTCAGA AAAAGAAGAT 
CCAATAAAAT ACAATGATTA CCTAGAAGTG CAAGAAATGA CCGACATCAC GTGGCTCAAC 
TCCCCCAGTC AGGTGGCGGT AGTGCCCTGG GGGTTGCGCA AAGTGCTGAA CTGGCTGAAG 
TTCAAGTACG GAGACCTCCC CATGTACATA ATATCCAATG GAATCGATGA CGGGCTGCAT 
GCTGAGGACG ACCAGCTGAG GGTGTATTAT ATGCAGAATT ACATAAACGA AGCTCTCAAA 
GCCCACATAC TGGATGGTAT CAATCTTTGC GGATACTTTG CTTATTCGTT TAACGACCGC 
ACAGCTCCGA GGTTTGGCCT CTATCGTTAT GCTGCAGATC AGTTTGAGCC CAAGGCATCC 
ATGAAACATT ACAGGAAAAT TATTGACAGC AATGGTTTCC CGGGCCCAGA AACTCTGGAA 
AGATTTTGTC CAGAAGAATT CACCGTGTGT ACTGAGTGCA GTTTTTTTCA CACCCGAAAG 
TCTTTACTGG CTTTCATAGC TTTTCTATTT TTTGCTTCTA TTATTTCTCT CTCCCTTATA 
TTTTACTACT CGAAGAAAGG CAGAAGAAGT TACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004786.2
CDS	9..3047
Translation	MPASAPPRRPRPPPPSLSLLLVLLGLGGRRLRAEPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMASELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVKLWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACPFSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTECSFFHTRKSLLAFIAFLFFASIISLSLIFYYSKKGRRSYK

Target ORF information:

RefSeq Version	NM_004795.3
Organism	Homo sapiens(human)
Definition	Homo sapiens klotho (KL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004795.3

ORF Insert Sequence:

ATGCCCGCCA GCGCCCCGCC GCGCCGCCCG CGGCCGCCGC CGCCGTCGCT GTCGCTGCTG 
CTGGTGCTGC TGGGCCTGGG CGGCCGCCGC CTGCGTGCGG AGCCGGGCGA CGGCGCGCAG 
ACCTGGGCCC GTTTCTCGCG GCCTCCTGCC CCCGAGGCCG CGGGCCTCTT CCAGGGCACC 
TTCCCCGACG GCTTCCTCTG GGCCGTGGGC AGCGCCGCCT ACCAGACCGA GGGCGGCTGG 
CAGCAGCACG GCAAGGGTGC GTCCATCTGG GATACGTTCA CCCACCACCC CCTGGCACCC 
CCGGGAGACT CCCGGAACGC CAGTCTGCCG TTGGGCGCCC CGTCGCCGCT GCAGCCCGCC 
ACCGGGGACG TAGCCAGCGA CAGCTACAAC AACGTCTTCC GCGACACGGA GGCGCTGCGC 
GAGCTCGGGG TCACTCACTA CCGCTTCTCC ATCTCGTGGG CGCGAGTGCT CCCCAATGGC 
AGCGCGGGCG TCCCCAACCG CGAGGGGCTG CGCTACTACC GGCGCCTGCT GGAGCGGCTG 
CGGGAGCTGG GCGTGCAGCC CGTGGTCACC CTGTACCACT GGGACCTGCC CCAGCGCCTG 
CAGGACGCCT ACGGCGGCTG GGCCAACCGC GCCCTGGCCG ACCACTTCAG GGATTACGCG 
GAGCTCTGCT TCCGCCACTT CGGCGGTCAG GTCAAGTACT GGATCACCAT CGACAACCCC 
TACGTGGTGG CCTGGCACGG CTACGCCACC GGGCGCCTGG CCCCCGGCAT CCGGGGCAGC 
CCGCGGCTCG GGTACCTGGT GGCGCACAAC CTCCTCCTGG CTCATGCCAA AGTCTGGCAT 
CTCTACAATA CTTCTTTCCG TCCCACTCAG GGAGGTCAGG TGTCCATTGC CCTAAGCTCT 
CACTGGATCA ATCCTCGAAG AATGACCGAC CACAGCATCA AAGAATGTCA AAAATCTCTG 
GACTTTGTAC TAGGTTGGTT TGCCAAACCC GTATTTATTG ATGGTGACTA TCCCGAGAGC 
ATGAAGAATA ACCTTTCATC TATTCTGCCT GATTTTACTG AATCTGAGAA AAAGTTCATC 
AAAGGAACTG CTGACTTTTT TGCTCTTTGC TTTGGACCCA CCTTGAGTTT TCAACTTTTG 
GACCCTCACA TGAAGTTCCG CCAATTGGAA TCTCCCAACC TGAGGCAACT GCTTTCCTGG 
ATTGACCTTG AATTTAACCA TCCTCAAATA TTTATTGTGG AAAATGGCTG GTTTGTCTCA 
GGGACCACCA AGAGAGATGA TGCCAAATAT ATGTATTACC TCAAAAAGTT CATCATGGAA 
ACCTTAAAAG CCATCAAGCT GGATGGGGTG GATGTCATCG GGTATACCGC ATGGTCCCTC 
ATGGATGGTT TCGAGTGGCA CAGAGGTTAC AGCATCAGGC GTGGACTCTT CTATGTTGAC 
TTTCTAAGCC AGGACAAGAT GTTGTTGCCA AAGTCTTCAG CCTTGTTCTA CCAAAAGCTG 
ATAGAGAAAA ATGGCTTCCC TCCTTTACCT GAAAATCAGC CCCTAGAAGG GACATTTCCC 
TGTGACTTTG CTTGGGGAGT TGTTGACAAC TACATTCAAG TAGATACCAC TCTGTCTCAG 
TTTACCGACC TGAATGTTTA CCTGTGGGAT GTCCACCACA GTAAAAGGCT TATTAAAGTG 
GATGGGGTTG TGACCAAGAA GAGGAAATCC TACTGTGTTG ACTTTGCTGC CATCCAGCCC 
CAGATCGCTT TACTCCAGGA AATGCACGTT ACACATTTTC GCTTCTCCCT GGACTGGGCC 
CTGATTCTCC CTCTGGGTAA CCAGTCCCAG GTGAACCACA CCATCCTGCA GTACTATCGC 
TGCATGGCCA GCGAGCTTGT CCGTGTCAAC ATCACCCCAG TGGTGGCCCT GTGGCAGCCT 
ATGGCCCCGA ACCAAGGACT GCCGCGCCTC CTGGCCAGGC AGGGCGCCTG GGAGAACCCC 
TACACTGCCC TGGCCTTTGC AGAGTATGCC CGACTGTGCT TTCAAGAGCT CGGCCATCAC 
GTCAAGCTTT GGATAACGAT GAATGAGCCG TATACAAGGA ATATGACATA CAGTGCTGGC 
CACAACCTTC TGAAGGCCCA TGCCCTGGCT TGGCATGTGT ACAATGAAAA GTTTAGGCAT 
GCTCAGAATG GGAAAATATC CATAGCCTTG CAGGCTGATT GGATAGAACC TGCCTGCCCT 
TTCTCCCAAA AGGACAAAGA GGTGGCTGAG AGAGTTTTGG AATTTGACAT TGGCTGGCTG 
GCTGAGCCCA TTTTCGGCTC TGGAGATTAT CCATGGGTGA TGAGGGACTG GCTGAACCAA 
AGAAACAATT TTCTTCTTCC TTATTTCACT GAAGATGAAA AAAAGCTAAT CCAGGGTACC 
TTTGACTTTT TGGCTTTAAG CCATTATACC ACCATCCTTG TAGACTCAGA AAAAGAAGAT 
CCAATAAAAT ACAATGATTA CCTAGAAGTG CAAGAAATGA CCGACATCAC GTGGCTCAAC 
TCCCCCAGTC AGGTGGCGGT AGTGCCCTGG GGGTTGCGCA AAGTGCTGAA CTGGCTGAAG 
TTCAAGTACG GAGACCTCCC CATGTACATA ATATCCAATG GAATCGATGA CGGGCTGCAT 
GCTGAGGACG ACCAGCTGAG GGTGTATTAT ATGCAGAATT ACATAAACGA AGCTCTCAAA 
GCCCACATAC TGGATGGTAT CAATCTTTGC GGATACTTTG CTTATTCGTT TAACGACCGC 
ACAGCTCCGA GGTTTGGCCT CTATCGTTAT GCTGCAGATC AGTTTGAGCC CAAGGCATCC 
ATGAAACATT ACAGGAAAAT TATTGACAGC AATGGTTTCC CGGGCCCAGA AACTCTGGAA 
AGATTTTGTC CAGAAGAATT CACCGTGTGT ACTGAGTGCA GTTTTTTTCA CACCCGAAAG 
TCTTTACTGG CTTTCATAGC TTTTCTATTT TTTGCTTCTA TTATTTCTCT CTCCCTTATA 
TTTTACTACT CGAAGAAAGG CAGAAGAAGT TACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KL ( NM_004795.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004786.2 Homo sapiens klotho (KL), mRNA.

Price & Availability↑

CloneID	OHu18278
Clone ID Related Accession (Same CDS sequence)	NM_004795.3 , NM_004795.4
Accession Version	NM_004795.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	klotho precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161898.17, AB005142.1 and AA890465.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004795.3. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB005142.1, SRR1803611.233700.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000380099.4/ ENSP00000369442.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGCCA GCGCCCCGCC GCGCCGCCCG CGGCCGCCGC CGCCGTCGCT GTCGCTGCTG 
CTGGTGCTGC TGGGCCTGGG CGGCCGCCGC CTGCGTGCGG AGCCGGGCGA CGGCGCGCAG 
ACCTGGGCCC GTTTCTCGCG GCCTCCTGCC CCCGAGGCCG CGGGCCTCTT CCAGGGCACC 
TTCCCCGACG GCTTCCTCTG GGCCGTGGGC AGCGCCGCCT ACCAGACCGA GGGCGGCTGG 
CAGCAGCACG GCAAGGGTGC GTCCATCTGG GATACGTTCA CCCACCACCC CCTGGCACCC 
CCGGGAGACT CCCGGAACGC CAGTCTGCCG TTGGGCGCCC CGTCGCCGCT GCAGCCCGCC 
ACCGGGGACG TAGCCAGCGA CAGCTACAAC AACGTCTTCC GCGACACGGA GGCGCTGCGC 
GAGCTCGGGG TCACTCACTA CCGCTTCTCC ATCTCGTGGG CGCGAGTGCT CCCCAATGGC 
AGCGCGGGCG TCCCCAACCG CGAGGGGCTG CGCTACTACC GGCGCCTGCT GGAGCGGCTG 
CGGGAGCTGG GCGTGCAGCC CGTGGTCACC CTGTACCACT GGGACCTGCC CCAGCGCCTG 
CAGGACGCCT ACGGCGGCTG GGCCAACCGC GCCCTGGCCG ACCACTTCAG GGATTACGCG 
GAGCTCTGCT TCCGCCACTT CGGCGGTCAG GTCAAGTACT GGATCACCAT CGACAACCCC 
TACGTGGTGG CCTGGCACGG CTACGCCACC GGGCGCCTGG CCCCCGGCAT CCGGGGCAGC 
CCGCGGCTCG GGTACCTGGT GGCGCACAAC CTCCTCCTGG CTCATGCCAA AGTCTGGCAT 
CTCTACAATA CTTCTTTCCG TCCCACTCAG GGAGGTCAGG TGTCCATTGC CCTAAGCTCT 
CACTGGATCA ATCCTCGAAG AATGACCGAC CACAGCATCA AAGAATGTCA AAAATCTCTG 
GACTTTGTAC TAGGTTGGTT TGCCAAACCC GTATTTATTG ATGGTGACTA TCCCGAGAGC 
ATGAAGAATA ACCTTTCATC TATTCTGCCT GATTTTACTG AATCTGAGAA AAAGTTCATC 
AAAGGAACTG CTGACTTTTT TGCTCTTTGC TTTGGACCCA CCTTGAGTTT TCAACTTTTG 
GACCCTCACA TGAAGTTCCG CCAATTGGAA TCTCCCAACC TGAGGCAACT GCTTTCCTGG 
ATTGACCTTG AATTTAACCA TCCTCAAATA TTTATTGTGG AAAATGGCTG GTTTGTCTCA 
GGGACCACCA AGAGAGATGA TGCCAAATAT ATGTATTACC TCAAAAAGTT CATCATGGAA 
ACCTTAAAAG CCATCAAGCT GGATGGGGTG GATGTCATCG GGTATACCGC ATGGTCCCTC 
ATGGATGGTT TCGAGTGGCA CAGAGGTTAC AGCATCAGGC GTGGACTCTT CTATGTTGAC 
TTTCTAAGCC AGGACAAGAT GTTGTTGCCA AAGTCTTCAG CCTTGTTCTA CCAAAAGCTG 
ATAGAGAAAA ATGGCTTCCC TCCTTTACCT GAAAATCAGC CCCTAGAAGG GACATTTCCC 
TGTGACTTTG CTTGGGGAGT TGTTGACAAC TACATTCAAG TAGATACCAC TCTGTCTCAG 
TTTACCGACC TGAATGTTTA CCTGTGGGAT GTCCACCACA GTAAAAGGCT TATTAAAGTG 
GATGGGGTTG TGACCAAGAA GAGGAAATCC TACTGTGTTG ACTTTGCTGC CATCCAGCCC 
CAGATCGCTT TACTCCAGGA AATGCACGTT ACACATTTTC GCTTCTCCCT GGACTGGGCC 
CTGATTCTCC CTCTGGGTAA CCAGTCCCAG GTGAACCACA CCATCCTGCA GTACTATCGC 
TGCATGGCCA GCGAGCTTGT CCGTGTCAAC ATCACCCCAG TGGTGGCCCT GTGGCAGCCT 
ATGGCCCCGA ACCAAGGACT GCCGCGCCTC CTGGCCAGGC AGGGCGCCTG GGAGAACCCC 
TACACTGCCC TGGCCTTTGC AGAGTATGCC CGACTGTGCT TTCAAGAGCT CGGCCATCAC 
GTCAAGCTTT GGATAACGAT GAATGAGCCG TATACAAGGA ATATGACATA CAGTGCTGGC 
CACAACCTTC TGAAGGCCCA TGCCCTGGCT TGGCATGTGT ACAATGAAAA GTTTAGGCAT 
GCTCAGAATG GGAAAATATC CATAGCCTTG CAGGCTGATT GGATAGAACC TGCCTGCCCT 
TTCTCCCAAA AGGACAAAGA GGTGGCTGAG AGAGTTTTGG AATTTGACAT TGGCTGGCTG 
GCTGAGCCCA TTTTCGGCTC TGGAGATTAT CCATGGGTGA TGAGGGACTG GCTGAACCAA 
AGAAACAATT TTCTTCTTCC TTATTTCACT GAAGATGAAA AAAAGCTAAT CCAGGGTACC 
TTTGACTTTT TGGCTTTAAG CCATTATACC ACCATCCTTG TAGACTCAGA AAAAGAAGAT 
CCAATAAAAT ACAATGATTA CCTAGAAGTG CAAGAAATGA CCGACATCAC GTGGCTCAAC 
TCCCCCAGTC AGGTGGCGGT AGTGCCCTGG GGGTTGCGCA AAGTGCTGAA CTGGCTGAAG 
TTCAAGTACG GAGACCTCCC CATGTACATA ATATCCAATG GAATCGATGA CGGGCTGCAT 
GCTGAGGACG ACCAGCTGAG GGTGTATTAT ATGCAGAATT ACATAAACGA AGCTCTCAAA 
GCCCACATAC TGGATGGTAT CAATCTTTGC GGATACTTTG CTTATTCGTT TAACGACCGC 
ACAGCTCCGA GGTTTGGCCT CTATCGTTAT GCTGCAGATC AGTTTGAGCC CAAGGCATCC 
ATGAAACATT ACAGGAAAAT TATTGACAGC AATGGTTTCC CGGGCCCAGA AACTCTGGAA 
AGATTTTGTC CAGAAGAATT CACCGTGTGT ACTGAGTGCA GTTTTTTTCA CACCCGAAAG 
TCTTTACTGG CTTTCATAGC TTTTCTATTT TTTGCTTCTA TTATTTCTCT CTCCCTTATA 
TTTTACTACT CGAAGAAAGG CAGAAGAAGT TACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004786.2
CDS	19..3057
Translation	MPASAPPRRPRPPPPSLSLLLVLLGLGGRRLRAEPGDGAQTWARFSRPPAPEAAGLFQGTFPDGFLWAVGSAAYQTEGGWQQHGKGASIWDTFTHHPLAPPGDSRNASLPLGAPSPLQPATGDVASDSYNNVFRDTEALRELGVTHYRFSISWARVLPNGSAGVPNREGLRYYRRLLERLRELGVQPVVTLYHWDLPQRLQDAYGGWANRALADHFRDYAELCFRHFGGQVKYWITIDNPYVVAWHGYATGRLAPGIRGSPRLGYLVAHNLLLAHAKVWHLYNTSFRPTQGGQVSIALSSHWINPRRMTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMASELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVKLWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACPFSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTECSFFHTRKSLLAFIAFLFFASIISLSLIFYYSKKGRRSYK

Target ORF information:

RefSeq Version	NM_004795.4
Organism	Homo sapiens(human)
Definition	Homo sapiens klotho (KL), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004795.4

ORF Insert Sequence:

ATGCCCGCCA GCGCCCCGCC GCGCCGCCCG CGGCCGCCGC CGCCGTCGCT GTCGCTGCTG 
CTGGTGCTGC TGGGCCTGGG CGGCCGCCGC CTGCGTGCGG AGCCGGGCGA CGGCGCGCAG 
ACCTGGGCCC GTTTCTCGCG GCCTCCTGCC CCCGAGGCCG CGGGCCTCTT CCAGGGCACC 
TTCCCCGACG GCTTCCTCTG GGCCGTGGGC AGCGCCGCCT ACCAGACCGA GGGCGGCTGG 
CAGCAGCACG GCAAGGGTGC GTCCATCTGG GATACGTTCA CCCACCACCC CCTGGCACCC 
CCGGGAGACT CCCGGAACGC CAGTCTGCCG TTGGGCGCCC CGTCGCCGCT GCAGCCCGCC 
ACCGGGGACG TAGCCAGCGA CAGCTACAAC AACGTCTTCC GCGACACGGA GGCGCTGCGC 
GAGCTCGGGG TCACTCACTA CCGCTTCTCC ATCTCGTGGG CGCGAGTGCT CCCCAATGGC 
AGCGCGGGCG TCCCCAACCG CGAGGGGCTG CGCTACTACC GGCGCCTGCT GGAGCGGCTG 
CGGGAGCTGG GCGTGCAGCC CGTGGTCACC CTGTACCACT GGGACCTGCC CCAGCGCCTG 
CAGGACGCCT ACGGCGGCTG GGCCAACCGC GCCCTGGCCG ACCACTTCAG GGATTACGCG 
GAGCTCTGCT TCCGCCACTT CGGCGGTCAG GTCAAGTACT GGATCACCAT CGACAACCCC 
TACGTGGTGG CCTGGCACGG CTACGCCACC GGGCGCCTGG CCCCCGGCAT CCGGGGCAGC 
CCGCGGCTCG GGTACCTGGT GGCGCACAAC CTCCTCCTGG CTCATGCCAA AGTCTGGCAT 
CTCTACAATA CTTCTTTCCG TCCCACTCAG GGAGGTCAGG TGTCCATTGC CCTAAGCTCT 
CACTGGATCA ATCCTCGAAG AATGACCGAC CACAGCATCA AAGAATGTCA AAAATCTCTG 
GACTTTGTAC TAGGTTGGTT TGCCAAACCC GTATTTATTG ATGGTGACTA TCCCGAGAGC 
ATGAAGAATA ACCTTTCATC TATTCTGCCT GATTTTACTG AATCTGAGAA AAAGTTCATC 
AAAGGAACTG CTGACTTTTT TGCTCTTTGC TTTGGACCCA CCTTGAGTTT TCAACTTTTG 
GACCCTCACA TGAAGTTCCG CCAATTGGAA TCTCCCAACC TGAGGCAACT GCTTTCCTGG 
ATTGACCTTG AATTTAACCA TCCTCAAATA TTTATTGTGG AAAATGGCTG GTTTGTCTCA 
GGGACCACCA AGAGAGATGA TGCCAAATAT ATGTATTACC TCAAAAAGTT CATCATGGAA 
ACCTTAAAAG CCATCAAGCT GGATGGGGTG GATGTCATCG GGTATACCGC ATGGTCCCTC 
ATGGATGGTT TCGAGTGGCA CAGAGGTTAC AGCATCAGGC GTGGACTCTT CTATGTTGAC 
TTTCTAAGCC AGGACAAGAT GTTGTTGCCA AAGTCTTCAG CCTTGTTCTA CCAAAAGCTG 
ATAGAGAAAA ATGGCTTCCC TCCTTTACCT GAAAATCAGC CCCTAGAAGG GACATTTCCC 
TGTGACTTTG CTTGGGGAGT TGTTGACAAC TACATTCAAG TAGATACCAC TCTGTCTCAG 
TTTACCGACC TGAATGTTTA CCTGTGGGAT GTCCACCACA GTAAAAGGCT TATTAAAGTG 
GATGGGGTTG TGACCAAGAA GAGGAAATCC TACTGTGTTG ACTTTGCTGC CATCCAGCCC 
CAGATCGCTT TACTCCAGGA AATGCACGTT ACACATTTTC GCTTCTCCCT GGACTGGGCC 
CTGATTCTCC CTCTGGGTAA CCAGTCCCAG GTGAACCACA CCATCCTGCA GTACTATCGC 
TGCATGGCCA GCGAGCTTGT CCGTGTCAAC ATCACCCCAG TGGTGGCCCT GTGGCAGCCT 
ATGGCCCCGA ACCAAGGACT GCCGCGCCTC CTGGCCAGGC AGGGCGCCTG GGAGAACCCC 
TACACTGCCC TGGCCTTTGC AGAGTATGCC CGACTGTGCT TTCAAGAGCT CGGCCATCAC 
GTCAAGCTTT GGATAACGAT GAATGAGCCG TATACAAGGA ATATGACATA CAGTGCTGGC 
CACAACCTTC TGAAGGCCCA TGCCCTGGCT TGGCATGTGT ACAATGAAAA GTTTAGGCAT 
GCTCAGAATG GGAAAATATC CATAGCCTTG CAGGCTGATT GGATAGAACC TGCCTGCCCT 
TTCTCCCAAA AGGACAAAGA GGTGGCTGAG AGAGTTTTGG AATTTGACAT TGGCTGGCTG 
GCTGAGCCCA TTTTCGGCTC TGGAGATTAT CCATGGGTGA TGAGGGACTG GCTGAACCAA 
AGAAACAATT TTCTTCTTCC TTATTTCACT GAAGATGAAA AAAAGCTAAT CCAGGGTACC 
TTTGACTTTT TGGCTTTAAG CCATTATACC ACCATCCTTG TAGACTCAGA AAAAGAAGAT 
CCAATAAAAT ACAATGATTA CCTAGAAGTG CAAGAAATGA CCGACATCAC GTGGCTCAAC 
TCCCCCAGTC AGGTGGCGGT AGTGCCCTGG GGGTTGCGCA AAGTGCTGAA CTGGCTGAAG 
TTCAAGTACG GAGACCTCCC CATGTACATA ATATCCAATG GAATCGATGA CGGGCTGCAT 
GCTGAGGACG ACCAGCTGAG GGTGTATTAT ATGCAGAATT ACATAAACGA AGCTCTCAAA 
GCCCACATAC TGGATGGTAT CAATCTTTGC GGATACTTTG CTTATTCGTT TAACGACCGC 
ACAGCTCCGA GGTTTGGCCT CTATCGTTAT GCTGCAGATC AGTTTGAGCC CAAGGCATCC 
ATGAAACATT ACAGGAAAAT TATTGACAGC AATGGTTTCC CGGGCCCAGA AACTCTGGAA 
AGATTTTGTC CAGAAGAATT CACCGTGTGT ACTGAGTGCA GTTTTTTTCA CACCCGAAAG 
TCTTTACTGG CTTTCATAGC TTTTCTATTT TTTGCTTCTA TTATTTCTCT CTCCCTTATA 
TTTTACTACT CGAAGAAAGG CAGAAGAAGT TACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KL ( XM_006719895.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719958.1 Homo sapiens klotho (KL), transcript variant X1, mRNA.

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CloneID	OHu47313
Clone ID Related Accession (Same CDS sequence)	XM_006719895.2
Accession Version	XM_006719895.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	klotho isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719895.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGACC ACAGCATCAA AGAATGTCAA AAATCTCTGG ACTTTGTACT AGGTTGGTTT 
GCCAAACCCG TATTTATTGA TGGTGACTAT CCCGAGAGCA TGAAGAATAA CCTTTCATCT 
ATTCTGCCTG ATTTTACTGA ATCTGAGAAA AAGTTCATCA AAGGAACTGC TGACTTTTTT 
GCTCTTTGCT TTGGACCCAC CTTGAGTTTT CAACTTTTGG ACCCTCACAT GAAGTTCCGC 
CAATTGGAAT CTCCCAACCT GAGGCAACTG CTTTCCTGGA TTGACCTTGA ATTTAACCAT 
CCTCAAATAT TTATTGTGGA AAATGGCTGG TTTGTCTCAG GGACCACCAA GAGAGATGAT 
GCCAAATATA TGTATTACCT CAAAAAGTTC ATCATGGAAA CCTTAAAAGC CATCAAGCTG 
GATGGGGTGG ATGTCATCGG GTATACCGCA TGGTCCCTCA TGGATGGTTT CGAGTGGCAC 
AGAGGTTACA GCATCAGGCG TGGACTCTTC TATGTTGACT TTCTAAGCCA GGACAAGATG 
TTGTTGCCAA AGTCTTCAGC CTTGTTCTAC CAAAAGCTGA TAGAGAAAAA TGGCTTCCCT 
CCTTTACCTG AAAATCAGCC CCTAGAAGGG ACATTTCCCT GTGACTTTGC TTGGGGAGTT 
GTTGACAACT ACATTCAAGT AGATACCACT CTGTCTCAGT TTACCGACCT GAATGTTTAC 
CTGTGGGATG TCCACCACAG TAAAAGGCTT ATTAAAGTGG ATGGGGTTGT GACCAAGAAG 
AGGAAATCCT ACTGTGTTGA CTTTGCTGCC ATCCAGCCCC AGATCGCTTT ACTCCAGGAA 
ATGCACGTTA CACATTTTCG CTTCTCCCTG GACTGGGCCC TGATTCTCCC TCTGGGTAAC 
CAGTCCCAGG TGAACCACAC CATCCTGCAG TACTATCGCT GCATGGCCAG CGAGCTTGTC 
CGTGTCAACA TCACCCCAGT GGTGGCCCTG TGGCAGCCTA TGGCCCCGAA CCAAGGACTG 
CCGCGCCTCC TGGCCAGGCA GGGCGCCTGG GAGAACCCCT ACACTGCCCT GGCCTTTGCA 
GAGTATGCCC GACTGTGCTT TCAAGAGCTC GGCCATCACG TCAAGCTTTG GATAACGATG 
AATGAGCCGT ATACAAGGAA TATGACATAC AGTGCTGGCC ACAACCTTCT GAAGGCCCAT 
GCCCTGGCTT GGCATGTGTA CAATGAAAAG TTTAGGCATG CTCAGAATGG GAAAATATCC 
ATAGCCTTGC AGGCTGATTG GATAGAACCT GCCTGCCCTT TCTCCCAAAA GGACAAAGAG 
GTGGCTGAGA GAGTTTTGGA ATTTGACATT GGCTGGCTGG CTGAGCCCAT TTTCGGCTCT 
GGAGATTATC CATGGGTGAT GAGGGACTGG CTGAACCAAA GAAACAATTT TCTTCTTCCT 
TATTTCACTG AAGATGAAAA AAAGCTAATC CAGGGTACCT TTGACTTTTT GGCTTTAAGC 
CATTATACCA CCATCCTTGT AGACTCAGAA AAAGAAGATC CAATAAAATA CAATGATTAC 
CTAGAAGTGC AAGAAATGAC CGACATCACG TGGCTCAACT CCCCCAGTCA GGTGGCGGTA 
GTGCCCTGGG GGTTGCGCAA AGTGCTGAAC TGGCTGAAGT TCAAGTACGG AGACCTCCCC 
ATGTACATAA TATCCAATGG AATCGATGAC GGGCTGCATG CTGAGGACGA CCAGCTGAGG 
GTGTATTATA TGCAGAATTA CATAAACGAA GCTCTCAAAG CCCACATACT GGATGGTATC 
AATCTTTGCG GATACTTTGC TTATTCGTTT AACGACCGCA CAGCTCCGAG GTTTGGCCTC 
TATCGTTATG CTGCAGATCA GTTTGAGCCC AAGGCATCCA TGAAACATTA CAGGAAAATT 
ATTGACAGCA ATGGTTTCCC GGGCCCAGAA ACTCTGGAAA GATTTTGTCC AGAAGAATTC 
ACCGTGTGTA CTGAGTGCAG TTTTTTTCAC ACCCGAAAGT CTTTACTGGC TTTCATAGCT 
TTTCTATTTT TTGCTTCTAT TATTTCTCTC TCCCTTATAT TTTACTACTC GAAGAAAGGC 
AGAAGAAGTT ACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719958.1
CDS	354..2471
Translation	MTDHSIKECQKSLDFVLGWFAKPVFIDGDYPESMKNNLSSILPDFTESEKKFIKGTADFFALCFGPTLSFQLLDPHMKFRQLESPNLRQLLSWIDLEFNHPQIFIVENGWFVSGTTKRDDAKYMYYLKKFIMETLKAIKLDGVDVIGYTAWSLMDGFEWHRGYSIRRGLFYVDFLSQDKMLLPKSSALFYQKLIEKNGFPPLPENQPLEGTFPCDFAWGVVDNYIQVDTTLSQFTDLNVYLWDVHHSKRLIKVDGVVTKKRKSYCVDFAAIQPQIALLQEMHVTHFRFSLDWALILPLGNQSQVNHTILQYYRCMASELVRVNITPVVALWQPMAPNQGLPRLLARQGAWENPYTALAFAEYARLCFQELGHHVKLWITMNEPYTRNMTYSAGHNLLKAHALAWHVYNEKFRHAQNGKISIALQADWIEPACPFSQKDKEVAERVLEFDIGWLAEPIFGSGDYPWVMRDWLNQRNNFLLPYFTEDEKKLIQGTFDFLALSHYTTILVDSEKEDPIKYNDYLEVQEMTDITWLNSPSQVAVVPWGLRKVLNWLKFKYGDLPMYIISNGIDDGLHAEDDQLRVYYMQNYINEALKAHILDGINLCGYFAYSFNDRTAPRFGLYRYAADQFEPKASMKHYRKIIDSNGFPGPETLERFCPEEFTVCTECSFFHTRKSLLAFIAFLFFASIISLSLIFYYSKKGRRSYK

Target ORF information:

RefSeq Version	XM_006719895.2
Organism	Homo sapiens(human)
Definition	Homo sapiens klotho (KL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719895.2

ORF Insert Sequence:

ATGACCGACC ACAGCATCAA AGAATGTCAA AAATCTCTGG ACTTTGTACT AGGTTGGTTT 
GCCAAACCCG TATTTATTGA TGGTGACTAT CCCGAGAGCA TGAAGAATAA CCTTTCATCT 
ATTCTGCCTG ATTTTACTGA ATCTGAGAAA AAGTTCATCA AAGGAACTGC TGACTTTTTT 
GCTCTTTGCT TTGGACCCAC CTTGAGTTTT CAACTTTTGG ACCCTCACAT GAAGTTCCGC 
CAATTGGAAT CTCCCAACCT GAGGCAACTG CTTTCCTGGA TTGACCTTGA ATTTAACCAT 
CCTCAAATAT TTATTGTGGA AAATGGCTGG TTTGTCTCAG GGACCACCAA GAGAGATGAT 
GCCAAATATA TGTATTACCT CAAAAAGTTC ATCATGGAAA CCTTAAAAGC CATCAAGCTG 
GATGGGGTGG ATGTCATCGG GTATACCGCA TGGTCCCTCA TGGATGGTTT CGAGTGGCAC 
AGAGGTTACA GCATCAGGCG TGGACTCTTC TATGTTGACT TTCTAAGCCA GGACAAGATG 
TTGTTGCCAA AGTCTTCAGC CTTGTTCTAC CAAAAGCTGA TAGAGAAAAA TGGCTTCCCT 
CCTTTACCTG AAAATCAGCC CCTAGAAGGG ACATTTCCCT GTGACTTTGC TTGGGGAGTT 
GTTGACAACT ACATTCAAGT AGATACCACT CTGTCTCAGT TTACCGACCT GAATGTTTAC 
CTGTGGGATG TCCACCACAG TAAAAGGCTT ATTAAAGTGG ATGGGGTTGT GACCAAGAAG 
AGGAAATCCT ACTGTGTTGA CTTTGCTGCC ATCCAGCCCC AGATCGCTTT ACTCCAGGAA 
ATGCACGTTA CACATTTTCG CTTCTCCCTG GACTGGGCCC TGATTCTCCC TCTGGGTAAC 
CAGTCCCAGG TGAACCACAC CATCCTGCAG TACTATCGCT GCATGGCCAG CGAGCTTGTC 
CGTGTCAACA TCACCCCAGT GGTGGCCCTG TGGCAGCCTA TGGCCCCGAA CCAAGGACTG 
CCGCGCCTCC TGGCCAGGCA GGGCGCCTGG GAGAACCCCT ACACTGCCCT GGCCTTTGCA 
GAGTATGCCC GACTGTGCTT TCAAGAGCTC GGCCATCACG TCAAGCTTTG GATAACGATG 
AATGAGCCGT ATACAAGGAA TATGACATAC AGTGCTGGCC ACAACCTTCT GAAGGCCCAT 
GCCCTGGCTT GGCATGTGTA CAATGAAAAG TTTAGGCATG CTCAGAATGG GAAAATATCC 
ATAGCCTTGC AGGCTGATTG GATAGAACCT GCCTGCCCTT TCTCCCAAAA GGACAAAGAG 
GTGGCTGAGA GAGTTTTGGA ATTTGACATT GGCTGGCTGG CTGAGCCCAT TTTCGGCTCT 
GGAGATTATC CATGGGTGAT GAGGGACTGG CTGAACCAAA GAAACAATTT TCTTCTTCCT 
TATTTCACTG AAGATGAAAA AAAGCTAATC CAGGGTACCT TTGACTTTTT GGCTTTAAGC 
CATTATACCA CCATCCTTGT AGACTCAGAA AAAGAAGATC CAATAAAATA CAATGATTAC 
CTAGAAGTGC AAGAAATGAC CGACATCACG TGGCTCAACT CCCCCAGTCA GGTGGCGGTA 
GTGCCCTGGG GGTTGCGCAA AGTGCTGAAC TGGCTGAAGT TCAAGTACGG AGACCTCCCC 
ATGTACATAA TATCCAATGG AATCGATGAC GGGCTGCATG CTGAGGACGA CCAGCTGAGG 
GTGTATTATA TGCAGAATTA CATAAACGAA GCTCTCAAAG CCCACATACT GGATGGTATC 
AATCTTTGCG GATACTTTGC TTATTCGTTT AACGACCGCA CAGCTCCGAG GTTTGGCCTC 
TATCGTTATG CTGCAGATCA GTTTGAGCCC AAGGCATCCA TGAAACATTA CAGGAAAATT 
ATTGACAGCA ATGGTTTCCC GGGCCCAGAA ACTCTGGAAA GATTTTGTCC AGAAGAATTC 
ACCGTGTGTA CTGAGTGCAG TTTTTTTCAC ACCCGAAAGT CTTTACTGGC TTTCATAGCT 
TTTCTATTTT TTGCTTCTAT TATTTCTCTC TCCCTTATAT TTTACTACTC GAAGAAAGGC 
AGAAGAAGTT ACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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KL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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