MYOCD cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYOCD
Entrez Gene ID	93649
Full Name	myocardin
Synonyms	MYCD
General protein information	Preferred Names myocardin Names myocardin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p12
Summary	This gene encodes a nuclear protein, which is expressed in heart, aorta, and in smooth muscle cell-containing tissues. It functions as a transcriptional co-activator of serum response factor (SRF) and modulates expression of cardiac and smooth muscle-specific SRF-target genes, and thus may play a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009].
Disorder MIM:	606127

mRNA and Protein(s)

mRNA	Protein	Name
XM_017025342.1	XP_016880831.1	myocardin isoform X1
NM_153604.3	NP_705832.1	myocardin isoform 2
XM_005256863.1	XP_005256920.1	myocardin isoform X2
NM_001146312.2	NP_001139784.1	myocardin isoform 1
XM_005256864.1	XP_005256921.1	myocardin isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYOCD	NP_001139784.1
	LOC718193	XP_002808163.1
Xenopus tropicalis (tropical clawed frog)	myocd	XP_004918467.1
Canis lupus familiaris (dog)	MYOCD	XP_005620116.1
Gallus gallus (chicken)	MYOCD	NP_001074184.1
Danio rerio (zebrafish)	LOC100331725	XP_005169482.1
Rattus norvegicus (Norway rat)	Myocd	NP_872608.1
Pan troglodytes (chimpanzee)	MYOCD	XP_511830.2
Bos taurus (cattle)	LOC539517	XP_002695856.3
Mus musculus (house mouse)	Myocd	NP_660118.3

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of cardiac myocyte cell death and differentiation by myocardin.
Gordon JW
Molecular and cellular biochemistry437(1-2)119-131(2018 Jan)

Myocardin inhibited the gap protein connexin 43 via promoted miR-206 to regulate vascular smooth muscle cell phenotypic switch.
Li H, Xiang Y, Fan LJ, Zhang XY, Li JP, Yu CX, Bao LY, Cao DS, Xing WB, Liao XH, Zhang TC
Gene61622-30(2017 Jun)

VEGF-A Stimulates STAT3 Activity via Nitrosylation of Myocardin to Regulate the Expression of Vascular Smooth Muscle Cell Differentiation Markers.
Liao XH, Xiang Y, Li H, Zheng L, Xu Y, Xi Yu C, Li JP, Zhang XY, Xing WB, Cao DS, Bao LY, Zhang TC
Scientific reports7(1)2660(2017 Jun)

Yes-Associated Protein Inhibits Transcription of Myocardin and Attenuates Differentiation of Vascular Smooth Muscle Cell from Cardiovascular Progenitor Cell Lineage.
Wang L, Qiu P, Jiao J, Hirai H, Xiong W, Zhang J, Zhu T, Ma PX, Chen YE, Yang B
Stem cells (Dayton, Ohio)35(2)351-361(2017 Feb)

In search of novel targets for heart disease: myocardin and myocardin-related transcriptional cofactors.
Mikhailov AT, Torrado M
Biochemistry research international2012973723(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrates that myocardin indirectly down-regulates Cx43 through its repressive action on miR-206 to regulate vascular smooth muscle cell phenotypic switch.
Title: Myocardin inhibited the gap protein connexin 43 via promoted miR-206 to regulate vascular smooth muscle cell phenotypic switch.

These results have revealed the roles for atrogin-1 in the regulation of smooth muscle contractility through enhancement of myocardin ubiquitylation/degradation and its transcriptional activity.
Title: Atrogin-1 Increases Smooth Muscle Contractility Through Myocardin Degradation.

YAP negatively regulates differentiation of Vascular smooth muscle cells (VSMCs) derived from cardiovascular progenitor cell (CVPC) by decreasing transcription of myocardin in a NKX2.5-dependent manner.
Title: Yes-Associated Protein Inhibits Transcription of Myocardin and Attenuates Differentiation of Vascular Smooth Muscle Cell from Cardiovascular Progenitor Cell Lineage.

Myocardin inhibits estrogen receptor alpha-mediated proliferation of human breast cancer cells via regulating MicroRNA expression.
Title: Myocardin inhibits estrogen receptor alpha-mediated proliferation of human breast cancer MCF-7 cells via regulating MicroRNA expression.

Key role for miR-214 in modulation of MEF2C-MYOCD-LMOD1 signaling.
Title: MEF2C-MYOCD and Leiomodin1 Suppression by miRNA-214 Promotes Smooth Muscle Cell Phenotype Switching in Pulmonary Arterial Hypertension.

The following MYOCD gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYOCD cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20739	NM_001146312.2 Latest version!	Homo sapiens myocardin (MYOCD), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu49971	XM_005256864.1 Latest version!	Homo sapiens myocardin (MYOCD), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu15776	NM_153604.3 Latest version!	Homo sapiens myocardin (MYOCD), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
OHu49970	XM_005256863.1 Latest version!	Homo sapiens myocardin (MYOCD), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu87645	XM_017025342.1 Latest version!	Homo sapiens myocardin (MYOCD), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYOCD ( NM_001146312.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139784.1 Homo sapiens myocardin (MYOCD), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20739
Clone ID Related Accession (Same CDS sequence)	NM_001146312.2
Accession Version	NM_001146312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2961bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	myocardin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA650534.1, AK292885.1, AC005358.1 and BQ000229.1. This sequence is a reference standard in the RefSeqGene project. On Nov 19, 2013 this sequence version replaced NM_001146312.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292885.1, AY764180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GGATCTTGCT 
TCTGGCTCAG AAAATGACAG AAATGACTCA GCCTCACAGC CCAGCCACCA GTCAGATGCG 
GGGAAGCAGG GGCTTGGCCC CCCCAGCACC CCCATAGCCG TGCATGCTGC TGTAAAGTCC 
AAATCCTTGG GTGACAGTAA GAACCGCCAC AAAAAGCCCA AGGACCCCAA GCCAAAGGTG 
AAGAAGCTTA AATATCACCA GTACATTCCC CCAGACCAGA AGGCAGAGAA GTCCCCTCCA 
CCTATGGACT CAGCCTACGC TCGGCTGCTC CAGCAACAGC AGCTGTTCCT GCAGCTCCAA 
ATCCTCAGCC AGCAGCAGCA GCAGCAGCAA CACCGATTCA GCTACCTAGG GATGCACCAA 
GCTCAGCTTA AGGAACCAAA TGAACAGATG GTCAGAAATC CAAACTCTTC TTCAACGCCA 
CTGAGCAATA CCCCCTTGTC TCCTGTCAAA AACAGTTTTT CTGGACAAAC TGGTGTCTCT 
TCTTTCAAAC CAGGCCCACT CCCACCTAAC CTGGATGATC TGAAGGTCTC TGAATTAAGA 
CAACAGCTTC GAATTCGGGG CTTGCCTGTG TCAGGCACCA AAACGGCTCT CATGGACCGG 
CTTCGACCCT TCCAGGACTG CTCTGGCAAC CCAGTGCCGA ACTTTGGGGA TATAACGACT 
GTCACTTTTC CTGTCACACC CAACACGCTG CCCAATTACC AGTCTTCCTC TTCTACCAGT 
GCCCTGTCCA ACGGCTTCTA CCACTTTGGC AGCACCAGCT CCAGCCCCCC GATCTCCCCA 
GCCTCCTCTG ACCTGTCAGT CGCTGGGTCC CTGCCGGACA CCTTCAATGA TGCCTCCCCC 
TCCTTCGGCC TGCACCCGTC CCCAGTCCAC GTGTGCACGG AGGAAAGTCT CATGAGCAGC 
CTGAATGGGG GCTCTGTTCC TTCTGAGCTG GATGGGCTGG ACTCCGAGAA GGACAAGATG 
CTGGTGGAGA AGCAGAAGGT GATCAATGAA CTCACCTGGA AACTCCAGCA AGAGCAGAGG 
CAGGTGGAGG AGCTGAGGAT GCAGCTTCAG AAGCAGAAAA GGAATAACTG TTCAGAGAAG 
AAGCCGCTGC CTTTCCTGGC TGCCTCCATC AAGCAGGAAG AGGCTGTCTC CAGCTGTCCT 
TTTGCATCCC AAGTACCTGT GAAAAGACAA AGCAGCAGCT CAGAGTGTCA CCCACCGGCT 
TGTGAAGCTG CTCAACTCCA GCCTCTTGGA AATGCTCATT GTGTGGAGTC CTCAGATCAA 
ACCAATGTAC TTTCTTCCAC ATTTCTCAGC CCCCAGTGTT CCCCTCAGCA TTCACCGCTG 
GGGGCTGTGA AAAGCCCACA GCACATCAGT TTGCCCCCAT CACCCAACAA CCCTCACTTT 
CTGCCCTCAT CCTCCGGGGC CCAGGGAGAA GGGCACAGGG TCTCCTCGCC CATCAGCAGC 
CAGGTGTGCA CTGCACAGAA CTCAGGAGCA CACGATGGCC ATCCTCCAAG CTTCTCTCCC 
CATTCTTCCA GCCTCCACCC GCCCTTCTCT GGAGCCCAAG CAGACAGCAG TCATGGTGCC 
GGGGGAAACC CTTGTCCCAA AAGCCCATGT GTACAGCAAA AGATGGCTGG TTTACACTCT 
TCTGATAAGG TGGGGCCAAA GTTTTCAATT CCATCCCCAA CTTTTTCTAA GTCAAGTTCA 
GCAATTTCAG AGGTAACACA GCCTCCATCC TATGAAGATG CCGTAAAGCA GCAAATGACC 
CGGAGTCAGC AGATGGATGA ACTCCTGGAC GTGCTTATTG AAAGCGGAGA AATGCCAGCA 
GACGCTAGAG AGGATCACTC ATGTCTTCAA AAAGTCCCAA AGATACCCAG ATCTTCCCGA 
AGTCCAACTG CTGTCCTCAC CAAGCCCTCG GCTTCCTTTG AACAAGCCTC TTCAGGCAGC 
CAGATCCCCT TTGATCCCTA TGCCACCGAC AGTGATGAGC ATCTTGAAGT CTTATTAAAT 
TCCCAGAGCC CCCTAGGAAA GATGAGTGAT GTCACCCTTC TAAAAATTGG GAGCGAAGAG 
CCTCACTTTG ATGGGATAAT GGATGGATTC TCTGGGAAGG CTGCAGAAGA CCTCTTCAAT 
GCACATGAGA TCTTGCCAGG CCCCCTCTCT CCAATGCAGA CACAGTTTTC ACCCTCTTCT 
GTGGACAGCA ATGGGCTGCA GTTAAGCTTC ACTGAATCTC CCTGGGAAAC CATGGAGTGG 
CTGGACCTCA CTCCGCCAAA TTCCACACCA GGCTTTAGCG CCCTCACCAC CAGCAGCCCC 
AGCATCTTCA ACATCGATTT CCTGGATGTC ACTGATCTCA ATTTGAATTC TTCCATGGAC 
CTTCACTTGC AGCAGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139784.1
CDS	300..3260
Translation	MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

Target ORF information:

RefSeq Version	NM_001146312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myocardin (MYOCD), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001146312.2

ORF Insert Sequence:

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GGATCTTGCT 
TCTGGCTCAG AAAATGACAG AAATGACTCA GCCTCACAGC CCAGCCACCA GTCAGATGCG 
GGGAAGCAGG GGCTTGGCCC CCCCAGCACC CCCATAGCCG TGCATGCTGC TGTAAAGTCC 
AAATCCTTGG GTGACAGTAA GAACCGCCAC AAAAAGCCCA AGGACCCCAA GCCAAAGGTG 
AAGAAGCTTA AATATCACCA GTACATTCCC CCAGACCAGA AGGCAGAGAA GTCCCCTCCA 
CCTATGGACT CAGCCTACGC TCGGCTGCTC CAGCAACAGC AGCTGTTCCT GCAGCTCCAA 
ATCCTCAGCC AGCAGCAGCA GCAGCAGCAA CACCGATTCA GCTACCTAGG GATGCACCAA 
GCTCAGCTTA AGGAACCAAA TGAACAGATG GTCAGAAATC CAAACTCTTC TTCAACGCCA 
CTGAGCAATA CCCCCTTGTC TCCTGTCAAA AACAGTTTTT CTGGACAAAC TGGTGTCTCT 
TCTTTCAAAC CAGGCCCACT CCCACCTAAC CTGGATGATC TGAAGGTCTC TGAATTAAGA 
CAACAGCTTC GAATTCGGGG CTTGCCTGTG TCAGGCACCA AAACGGCTCT CATGGACCGG 
CTTCGACCCT TCCAGGACTG CTCTGGCAAC CCAGTGCCGA ACTTTGGGGA TATAACGACT 
GTCACTTTTC CTGTCACACC CAACACGCTG CCCAATTACC AGTCTTCCTC TTCTACCAGT 
GCCCTGTCCA ACGGCTTCTA CCACTTTGGC AGCACCAGCT CCAGCCCCCC GATCTCCCCA 
GCCTCCTCTG ACCTGTCAGT CGCTGGGTCC CTGCCGGACA CCTTCAATGA TGCCTCCCCC 
TCCTTCGGCC TGCACCCGTC CCCAGTCCAC GTGTGCACGG AGGAAAGTCT CATGAGCAGC 
CTGAATGGGG GCTCTGTTCC TTCTGAGCTG GATGGGCTGG ACTCCGAGAA GGACAAGATG 
CTGGTGGAGA AGCAGAAGGT GATCAATGAA CTCACCTGGA AACTCCAGCA AGAGCAGAGG 
CAGGTGGAGG AGCTGAGGAT GCAGCTTCAG AAGCAGAAAA GGAATAACTG TTCAGAGAAG 
AAGCCGCTGC CTTTCCTGGC TGCCTCCATC AAGCAGGAAG AGGCTGTCTC CAGCTGTCCT 
TTTGCATCCC AAGTACCTGT GAAAAGACAA AGCAGCAGCT CAGAGTGTCA CCCACCGGCT 
TGTGAAGCTG CTCAACTCCA GCCTCTTGGA AATGCTCATT GTGTGGAGTC CTCAGATCAA 
ACCAATGTAC TTTCTTCCAC ATTTCTCAGC CCCCAGTGTT CCCCTCAGCA TTCACCGCTG 
GGGGCTGTGA AAAGCCCACA GCACATCAGT TTGCCCCCAT CACCCAACAA CCCTCACTTT 
CTGCCCTCAT CCTCCGGGGC CCAGGGAGAA GGGCACAGGG TCTCCTCGCC CATCAGCAGC 
CAGGTGTGCA CTGCACAGAA CTCAGGAGCA CACGATGGCC ATCCTCCAAG CTTCTCTCCC 
CATTCTTCCA GCCTCCACCC GCCCTTCTCT GGAGCCCAAG CAGACAGCAG TCATGGTGCC 
GGGGGAAACC CTTGTCCCAA AAGCCCATGT GTACAGCAAA AGATGGCTGG TTTACACTCT 
TCTGATAAGG TGGGGCCAAA GTTTTCAATT CCATCCCCAA CTTTTTCTAA GTCAAGTTCA 
GCAATTTCAG AGGTAACACA GCCTCCATCC TATGAAGATG CCGTAAAGCA GCAAATGACC 
CGGAGTCAGC AGATGGATGA ACTCCTGGAC GTGCTTATTG AAAGCGGAGA AATGCCAGCA 
GACGCTAGAG AGGATCACTC ATGTCTTCAA AAAGTCCCAA AGATACCCAG ATCTTCCCGA 
AGTCCAACTG CTGTCCTCAC CAAGCCCTCG GCTTCCTTTG AACAAGCCTC TTCAGGCAGC 
CAGATCCCCT TTGATCCCTA TGCCACCGAC AGTGATGAGC ATCTTGAAGT CTTATTAAAT 
TCCCAGAGCC CCCTAGGAAA GATGAGTGAT GTCACCCTTC TAAAAATTGG GAGCGAAGAG 
CCTCACTTTG ATGGGATAAT GGATGGATTC TCTGGGAAGG CTGCAGAAGA CCTCTTCAAT 
GCACATGAGA TCTTGCCAGG CCCCCTCTCT CCAATGCAGA CACAGTTTTC ACCCTCTTCT 
GTGGACAGCA ATGGGCTGCA GTTAAGCTTC ACTGAATCTC CCTGGGAAAC CATGGAGTGG 
CTGGACCTCA CTCCGCCAAA TTCCACACCA GGCTTTAGCG CCCTCACCAC CAGCAGCCCC 
AGCATCTTCA ACATCGATTT CCTGGATGTC ACTGATCTCA ATTTGAATTC TTCCATGGAC 
CTTCACTTGC AGCAGTGGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOCD ( XM_005256864.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256921.1 Homo sapiens myocardin (MYOCD), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu49971
Clone ID Related Accession (Same CDS sequence)	XM_005256864.1
Accession Version	XM_005256864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myocardin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACATAC TCCAAGCTTC CACTGCAGAG AGGTCCATTC CAACTGCTCA GATGAAGCTG 
AAAAGAGCCC GACTCGCCGA TGATCTCAAT GAAAAAATTG CTCTACGACC AGGGCCACTG 
GAGCTGGTGG AAAAAAACAT TCTTCCTGTG GATTCTGCTG TGAAAGAGGC CATAAAAGGT 
AACCAGGTGA GTTTCTCCAA ATCCACGGAT GCTTTTGCCT TTGAAGAGGA CAGCAGCAGC 
GATGGGCTTT CTCCGGATCA GACTCGAAGT GAAGACCCCC AAAACTCAGC GGGATCCCCG 
CCAGACGCTA AAGCCTCAGA TACCCCTTCG ACAGGTTCTC TGGGGACAAA CCAGGATCTT 
GCTTCTGGCT CAGAAAATGA CAGAAATGAC TCAGCCTCAC AGCCCAGCCA CCAGTCAGAT 
GCGGGGAAGC AGGGGCTTGG CCCCCCCAGC ACCCCCATAG CCGTGCATGC TGCTGTAAAG 
TCCAAATCCT TGGGTGACAG TAAGAACCGC CACAAAAAGC CCAAGGACCC CAAGCCAAAG 
GTGAAGAAGC TTAAATATCA CCAGTACATT CCCCCAGACC AGAAGGCAGA GAAGTCCCCT 
CCACCTATGG ACTCAGCCTA CGCTCGGCTG CTCCAGCAAC AGCAGCTGTT CCTGCAGCTC 
CAAATCCTCA GCCAGCAGCA GCAGCAGCAG CAACACCGAT TCAGCTACCT AGGGATGCAC 
CAAGCTCAGC TTAAGGAACC AAATGAACAG ATGGTCAGAA ATCCAAACTC TTCTTCAACG 
CCACTGAGCA ATACCCCCTT GTCTCCTGTC AAAAACAGTT TTTCTGGACA AACTGGTGTC 
TCTTCTTTCA AACCAGGCCC ACTCCCACCT AACCTGGATG ATCTGAAGGT CTCTGAATTA 
AGACAACAGC TTCGAATTCG GGGCTTGCCT GTGTCAGGCA CCAAAACGGC TCTCATGGAC 
CGGCTTCGAC CCTTCCAGGA CTGCTCTGGC AACCCAGTGC CGAACTTTGG GGATATAACG 
ACTGTCACTT TTCCTGTCAC ACCCAACACG CTGCCCAATT ACCAGTCTTC CTCTTCTACC 
AGTGCCCTGT CCAACGGCTT CTACCACTTT GGCAGCACCA GCTCCAGCCC CCCGATCTCC 
CCAGCCTCCT CTGACCTGTC AGTCGCTGGG TCCCTGCCGG ACACCTTCAA TGATGCCTCC 
CCCTCCTTCG GCCTGCACCC GTCCCCAGTC CACGTGTGCA CGGAGGAAAG TCTCATGAGC 
AGCCTGAATG GGGGCTCTGT TCCTTCTGAG CTGGATGGGC TGGACTCCGA GAAGGACAAG 
ATGCTGGTGG AGAAGCAGAA GGTGATCAAT GAACTCACCT GGAAACTCCA GCAAGAGCAG 
AGGCAGGTGG AGGAGCTGAG GATGCAGCTT CAGAAGCAGA AAAGGAATAA CTGTTCAGAG 
AAGAAGCCGC TGCCTTTCCT GGCTGCCTCC ATCAAGCAGG AAGAGGCTGT CTCCAGCTGT 
CCTTTTGCAT CCCAAGTACC TGTGAAAAGA CAAAGCAGCA GCTCAGAGTG TCACCCACCG 
GCTTGTGAAG CTGCTCAACT CCAGCCTCTT GGAAATGCTC ATTGTGTGGA GTCCTCAGAT 
CAAACCAATG TACTTTCTTC CACATTTCTC AGCCCCCAGT GTTCCCCTCA GCATTCACCG 
CTGGGGGCTG TGAAAAGCCC ACAGCACATC AGTTTGCCCC CATCACCCAA CAACCCTCAC 
TTTCTGCCCT CATCCTCCGG GGCCCAGGGA GAAGGGCACA GGGTCTCCTC GCCCATCAGC 
AGCCAGGTGT GCACTGCACA GAACTCAGGA GCACACGATG GCCATCCTCC AAGCTTCTCT 
CCCCATTCTT CCAGCCTCCA CCCGCCCTTC TCTGGAGCCC AAGCAGACAG CAGTCATGGT 
GCCGGGGGAA ACCCTTGTCC CAAAAGCCCA TGTGTACAGC AAAAGATGGC TGGTTTACAC 
TCTTCTGATA AGGTGGGGCC AAAGTTTTCA ATTCCATCCC CAACTTTTTC TAAGTCAAGT 
TCAGCAATTT CAGAGGTAAC ACAGCCTCCA TCCTATGAAG ATGCCGTAAA GCAGCAAATG 
ACCCGGAGTC AGCAGATGGA TGAACTCCTG GACGTGCTTA TTGAAAGCGG AGAAATGCCA 
GCAGACGCTA GAGAGGATCA CTCATGTCTT CAAAAAGTCC CAAAGATACC CAGATCTTCC 
CGAAGTCCAA CTGCTGTCCT CACCAAGCCC TCGGCTTCCT TTGAACAAGC CTCTTCAGGC 
AGCCAGATCC CCTTTGATCC CTATGCCACC GACAGTGATG AGCATCTTGA AGTCTTATTA 
AATTCCCAGA GCCCCCTAGG AAAGATGAGT GATGTCACCC TTCTAAAAAT TGGGAGCGAA 
GAGCCTCACT TTGATGGGAT AATGGATGGA TTCTCTGGGA AGGCTGCAGA AGACCTCTTC 
AATGCACATG AGATCTTGCC AGGCCCCCTC TCTCCAATGC AGACACAGTT TTCACCCTCT 
TCTGTGGACA GCAATGGGCT GCAGTTAAGC TTCACTGAAT CTCCCTGGGA AACCATGGAG 
TGGCTGGACC TCACTCCGCC AAATTCCACA CCAGGCTTTA GCGCCCTCAC CACCAGCAGC 
CCCAGCATCT TCAACATCGA TTTCCTGGAT GTCACTGATC TCAATTTGAA TTCTTCCATG 
GACCTTCACT TGCAGCAGTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256921.1
CDS	581..3304
Translation	MHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

Target ORF information:

RefSeq Version	XM_005256864.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myocardin (MYOCD), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256864.1

ORF Insert Sequence:

ATGCACATAC TCCAAGCTTC CACTGCAGAG AGGTCCATTC CAACTGCTCA GATGAAGCTG 
AAAAGAGCCC GACTCGCCGA TGATCTCAAT GAAAAAATTG CTCTACGACC AGGGCCACTG 
GAGCTGGTGG AAAAAAACAT TCTTCCTGTG GATTCTGCTG TGAAAGAGGC CATAAAAGGT 
AACCAGGTGA GTTTCTCCAA ATCCACGGAT GCTTTTGCCT TTGAAGAGGA CAGCAGCAGC 
GATGGGCTTT CTCCGGATCA GACTCGAAGT GAAGACCCCC AAAACTCAGC GGGATCCCCG 
CCAGACGCTA AAGCCTCAGA TACCCCTTCG ACAGGTTCTC TGGGGACAAA CCAGGATCTT 
GCTTCTGGCT CAGAAAATGA CAGAAATGAC TCAGCCTCAC AGCCCAGCCA CCAGTCAGAT 
GCGGGGAAGC AGGGGCTTGG CCCCCCCAGC ACCCCCATAG CCGTGCATGC TGCTGTAAAG 
TCCAAATCCT TGGGTGACAG TAAGAACCGC CACAAAAAGC CCAAGGACCC CAAGCCAAAG 
GTGAAGAAGC TTAAATATCA CCAGTACATT CCCCCAGACC AGAAGGCAGA GAAGTCCCCT 
CCACCTATGG ACTCAGCCTA CGCTCGGCTG CTCCAGCAAC AGCAGCTGTT CCTGCAGCTC 
CAAATCCTCA GCCAGCAGCA GCAGCAGCAG CAACACCGAT TCAGCTACCT AGGGATGCAC 
CAAGCTCAGC TTAAGGAACC AAATGAACAG ATGGTCAGAA ATCCAAACTC TTCTTCAACG 
CCACTGAGCA ATACCCCCTT GTCTCCTGTC AAAAACAGTT TTTCTGGACA AACTGGTGTC 
TCTTCTTTCA AACCAGGCCC ACTCCCACCT AACCTGGATG ATCTGAAGGT CTCTGAATTA 
AGACAACAGC TTCGAATTCG GGGCTTGCCT GTGTCAGGCA CCAAAACGGC TCTCATGGAC 
CGGCTTCGAC CCTTCCAGGA CTGCTCTGGC AACCCAGTGC CGAACTTTGG GGATATAACG 
ACTGTCACTT TTCCTGTCAC ACCCAACACG CTGCCCAATT ACCAGTCTTC CTCTTCTACC 
AGTGCCCTGT CCAACGGCTT CTACCACTTT GGCAGCACCA GCTCCAGCCC CCCGATCTCC 
CCAGCCTCCT CTGACCTGTC AGTCGCTGGG TCCCTGCCGG ACACCTTCAA TGATGCCTCC 
CCCTCCTTCG GCCTGCACCC GTCCCCAGTC CACGTGTGCA CGGAGGAAAG TCTCATGAGC 
AGCCTGAATG GGGGCTCTGT TCCTTCTGAG CTGGATGGGC TGGACTCCGA GAAGGACAAG 
ATGCTGGTGG AGAAGCAGAA GGTGATCAAT GAACTCACCT GGAAACTCCA GCAAGAGCAG 
AGGCAGGTGG AGGAGCTGAG GATGCAGCTT CAGAAGCAGA AAAGGAATAA CTGTTCAGAG 
AAGAAGCCGC TGCCTTTCCT GGCTGCCTCC ATCAAGCAGG AAGAGGCTGT CTCCAGCTGT 
CCTTTTGCAT CCCAAGTACC TGTGAAAAGA CAAAGCAGCA GCTCAGAGTG TCACCCACCG 
GCTTGTGAAG CTGCTCAACT CCAGCCTCTT GGAAATGCTC ATTGTGTGGA GTCCTCAGAT 
CAAACCAATG TACTTTCTTC CACATTTCTC AGCCCCCAGT GTTCCCCTCA GCATTCACCG 
CTGGGGGCTG TGAAAAGCCC ACAGCACATC AGTTTGCCCC CATCACCCAA CAACCCTCAC 
TTTCTGCCCT CATCCTCCGG GGCCCAGGGA GAAGGGCACA GGGTCTCCTC GCCCATCAGC 
AGCCAGGTGT GCACTGCACA GAACTCAGGA GCACACGATG GCCATCCTCC AAGCTTCTCT 
CCCCATTCTT CCAGCCTCCA CCCGCCCTTC TCTGGAGCCC AAGCAGACAG CAGTCATGGT 
GCCGGGGGAA ACCCTTGTCC CAAAAGCCCA TGTGTACAGC AAAAGATGGC TGGTTTACAC 
TCTTCTGATA AGGTGGGGCC AAAGTTTTCA ATTCCATCCC CAACTTTTTC TAAGTCAAGT 
TCAGCAATTT CAGAGGTAAC ACAGCCTCCA TCCTATGAAG ATGCCGTAAA GCAGCAAATG 
ACCCGGAGTC AGCAGATGGA TGAACTCCTG GACGTGCTTA TTGAAAGCGG AGAAATGCCA 
GCAGACGCTA GAGAGGATCA CTCATGTCTT CAAAAAGTCC CAAAGATACC CAGATCTTCC 
CGAAGTCCAA CTGCTGTCCT CACCAAGCCC TCGGCTTCCT TTGAACAAGC CTCTTCAGGC 
AGCCAGATCC CCTTTGATCC CTATGCCACC GACAGTGATG AGCATCTTGA AGTCTTATTA 
AATTCCCAGA GCCCCCTAGG AAAGATGAGT GATGTCACCC TTCTAAAAAT TGGGAGCGAA 
GAGCCTCACT TTGATGGGAT AATGGATGGA TTCTCTGGGA AGGCTGCAGA AGACCTCTTC 
AATGCACATG AGATCTTGCC AGGCCCCCTC TCTCCAATGC AGACACAGTT TTCACCCTCT 
TCTGTGGACA GCAATGGGCT GCAGTTAAGC TTCACTGAAT CTCCCTGGGA AACCATGGAG 
TGGCTGGACC TCACTCCGCC AAATTCCACA CCAGGCTTTA GCGCCCTCAC CACCAGCAGC 
CCCAGCATCT TCAACATCGA TTTCCTGGAT GTCACTGATC TCAATTTGAA TTCTTCCATG 
GACCTTCACT TGCAGCAGTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOCD ( NM_153604.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_705832.1 Homo sapiens myocardin (MYOCD), transcript variant 2, mRNA.

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CloneID	OHu15776
Clone ID Related Accession (Same CDS sequence)	NM_153604.3
Accession Version	NM_153604.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	myocardin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA650534.1, AF532596.1, AC005358.1 and BQ000229.1. On Nov 19, 2013 this sequence version replaced NM_153604.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' coding region, compared to variant 1, resulting in a shorter isoform (2) that lacks a 48 aa segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF532596.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465416 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GGATCTTGCT 
TCTGGCTCAG AAAATGACAG AAATGACTCA GCCTCACAGC CCAGCCACCA GTCAGATGCG 
GGGAAGCAGG GGCTTGGCCC CCCCAGCACC CCCATAGCCG TGCATGCTGC TGTAAAGTCC 
AAATCCTTGG GTGACAGTAA GAACCGCCAC AAAAAGCCCA AGGACCCCAA GCCAAAGGTG 
AAGAAGCTTA AATATCACCA GTACATTCCC CCAGACCAGA AGGCAGAGAA GTCCCCTCCA 
CCTATGGACT CAGCCTACGC TCGGCTGCTC CAGCAACAGC AGCTGTTCCT GCAGCTCCAA 
ATCCTCAGCC AGCAGCAGCA GCAGCAGCAA CACCGATTCA GCTACCTAGG GATGCACCAA 
GCTCAGCTTA AGGAACCAAA TGAACAGATG GTCAGAAATC CAAACTCTTC TTCAACGCCA 
CTGAGCAATA CCCCCTTGTC TCCTGTCAAA AACAGTTTTT CTGGACAAAC TGGTGTCTCT 
TCTTTCAAAC CAGGCCCACT CCCACCTAAC CTGGATGATC TGAAGGTCTC TGAATTAAGA 
CAACAGCTTC GAATTCGGGG CTTGCCTGTG TCAGGCACCA AAACGGCTCT CATGGACCGG 
CTTCGACCCT TCCAGGACTG CTCTGGCAAC CCAGTGCCGA ACTTTGGGGA TATAACGACT 
GTCACTTTTC CTGTCACACC CAACACGCTG CCCAATTACC AGTCTTCCTC TTCTACCAGT 
GCCCTGTCCA ACGGCTTCTA CCACTTTGGC AGCACCAGCT CCAGCCCCCC GATCTCCCCA 
GCCTCCTCTG ACCTGTCAGT CGCTGGGTCC CTGCCGGACA CCTTCAATGA TGCCTCCCCC 
TCCTTCGGCC TGCACCCGTC CCCAGTCCAC GTGTGCACGG AGGAAAGTCT CATGAGCAGC 
CTGAATGGGG GCTCTGTTCC TTCTGAGCTG GATGGGCTGG ACTCCGAGAA GGACAAGATG 
CTGGTGGAGA AGCAGAAGGT GATCAATGAA CTCACCTGGA AACTCCAGCA AGAGCAGAGG 
CAGGTGGAGG AGCTGAGGAT GCAGCTTCAG AAGCAGAAAA GGAATAACTG TTCAGAGAAG 
AAGCCGCTGC CTTTCCTGGC TGCCTCCATC AAGCAGGAAG AGGCTGTCTC CAGCTGTCCT 
TTTGCATCCC AAGTACCTGT GAAAAGACAA AGCAGCAGCT CAGAGTGTCA CCCACCGGCT 
TGTGAAGCTG CTCAACTCCA GCCTCTTGGA AATGCTCATT GTGTGGAGTC CTCAGATCAA 
ACCAATGTAC TTTCTTCCAC ATTTCTCAGC CCCCAGTGTT CCCCTCAGCA TTCACCGCTG 
GGGGCTGTGA AAAGCCCACA GCACATCAGT TTGCCCCCAT CACCCAACAA CCCTCACTTT 
CTGCCCTCAT CCTCCGGGGC CCAGGGAGAA GGGCACAGGG TCTCCTCGCC CATCAGCAGC 
CAGGTGTGCA CTGCACAGAT GGCTGGTTTA CACTCTTCTG ATAAGGTGGG GCCAAAGTTT 
TCAATTCCAT CCCCAACTTT TTCTAAGTCA AGTTCAGCAA TTTCAGAGGT AACACAGCCT 
CCATCCTATG AAGATGCCGT AAAGCAGCAA ATGACCCGGA GTCAGCAGAT GGATGAACTC 
CTGGACGTGC TTATTGAAAG CGGAGAAATG CCAGCAGACG CTAGAGAGGA TCACTCATGT 
CTTCAAAAAG TCCCAAAGAT ACCCAGATCT TCCCGAAGTC CAACTGCTGT CCTCACCAAG 
CCCTCGGCTT CCTTTGAACA AGCCTCTTCA GGCAGCCAGA TCCCCTTTGA TCCCTATGCC 
ACCGACAGTG ATGAGCATCT TGAAGTCTTA TTAAATTCCC AGAGCCCCCT AGGAAAGATG 
AGTGATGTCA CCCTTCTAAA AATTGGGAGC GAAGAGCCTC ACTTTGATGG GATAATGGAT 
GGATTCTCTG GGAAGGCTGC AGAAGACCTC TTCAATGCAC ATGAGATCTT GCCAGGCCCC 
CTCTCTCCAA TGCAGACACA GTTTTCACCC TCTTCTGTGG ACAGCAATGG GCTGCAGTTA 
AGCTTCACTG AATCTCCCTG GGAAACCATG GAGTGGCTGG ACCTCACTCC GCCAAATTCC 
ACACCAGGCT TTAGCGCCCT CACCACCAGC AGCCCCAGCA TCTTCAACAT CGATTTCCTG 
GATGTCACTG ATCTCAATTT GAATTCTTCC ATGGACCTTC ACTTGCAGCA GTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_705832.1
CDS	300..3116
Translation	MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

Target ORF information:

RefSeq Version	NM_153604.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myocardin (MYOCD), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153604.3

ORF Insert Sequence:

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GGATCTTGCT 
TCTGGCTCAG AAAATGACAG AAATGACTCA GCCTCACAGC CCAGCCACCA GTCAGATGCG 
GGGAAGCAGG GGCTTGGCCC CCCCAGCACC CCCATAGCCG TGCATGCTGC TGTAAAGTCC 
AAATCCTTGG GTGACAGTAA GAACCGCCAC AAAAAGCCCA AGGACCCCAA GCCAAAGGTG 
AAGAAGCTTA AATATCACCA GTACATTCCC CCAGACCAGA AGGCAGAGAA GTCCCCTCCA 
CCTATGGACT CAGCCTACGC TCGGCTGCTC CAGCAACAGC AGCTGTTCCT GCAGCTCCAA 
ATCCTCAGCC AGCAGCAGCA GCAGCAGCAA CACCGATTCA GCTACCTAGG GATGCACCAA 
GCTCAGCTTA AGGAACCAAA TGAACAGATG GTCAGAAATC CAAACTCTTC TTCAACGCCA 
CTGAGCAATA CCCCCTTGTC TCCTGTCAAA AACAGTTTTT CTGGACAAAC TGGTGTCTCT 
TCTTTCAAAC CAGGCCCACT CCCACCTAAC CTGGATGATC TGAAGGTCTC TGAATTAAGA 
CAACAGCTTC GAATTCGGGG CTTGCCTGTG TCAGGCACCA AAACGGCTCT CATGGACCGG 
CTTCGACCCT TCCAGGACTG CTCTGGCAAC CCAGTGCCGA ACTTTGGGGA TATAACGACT 
GTCACTTTTC CTGTCACACC CAACACGCTG CCCAATTACC AGTCTTCCTC TTCTACCAGT 
GCCCTGTCCA ACGGCTTCTA CCACTTTGGC AGCACCAGCT CCAGCCCCCC GATCTCCCCA 
GCCTCCTCTG ACCTGTCAGT CGCTGGGTCC CTGCCGGACA CCTTCAATGA TGCCTCCCCC 
TCCTTCGGCC TGCACCCGTC CCCAGTCCAC GTGTGCACGG AGGAAAGTCT CATGAGCAGC 
CTGAATGGGG GCTCTGTTCC TTCTGAGCTG GATGGGCTGG ACTCCGAGAA GGACAAGATG 
CTGGTGGAGA AGCAGAAGGT GATCAATGAA CTCACCTGGA AACTCCAGCA AGAGCAGAGG 
CAGGTGGAGG AGCTGAGGAT GCAGCTTCAG AAGCAGAAAA GGAATAACTG TTCAGAGAAG 
AAGCCGCTGC CTTTCCTGGC TGCCTCCATC AAGCAGGAAG AGGCTGTCTC CAGCTGTCCT 
TTTGCATCCC AAGTACCTGT GAAAAGACAA AGCAGCAGCT CAGAGTGTCA CCCACCGGCT 
TGTGAAGCTG CTCAACTCCA GCCTCTTGGA AATGCTCATT GTGTGGAGTC CTCAGATCAA 
ACCAATGTAC TTTCTTCCAC ATTTCTCAGC CCCCAGTGTT CCCCTCAGCA TTCACCGCTG 
GGGGCTGTGA AAAGCCCACA GCACATCAGT TTGCCCCCAT CACCCAACAA CCCTCACTTT 
CTGCCCTCAT CCTCCGGGGC CCAGGGAGAA GGGCACAGGG TCTCCTCGCC CATCAGCAGC 
CAGGTGTGCA CTGCACAGAT GGCTGGTTTA CACTCTTCTG ATAAGGTGGG GCCAAAGTTT 
TCAATTCCAT CCCCAACTTT TTCTAAGTCA AGTTCAGCAA TTTCAGAGGT AACACAGCCT 
CCATCCTATG AAGATGCCGT AAAGCAGCAA ATGACCCGGA GTCAGCAGAT GGATGAACTC 
CTGGACGTGC TTATTGAAAG CGGAGAAATG CCAGCAGACG CTAGAGAGGA TCACTCATGT 
CTTCAAAAAG TCCCAAAGAT ACCCAGATCT TCCCGAAGTC CAACTGCTGT CCTCACCAAG 
CCCTCGGCTT CCTTTGAACA AGCCTCTTCA GGCAGCCAGA TCCCCTTTGA TCCCTATGCC 
ACCGACAGTG ATGAGCATCT TGAAGTCTTA TTAAATTCCC AGAGCCCCCT AGGAAAGATG 
AGTGATGTCA CCCTTCTAAA AATTGGGAGC GAAGAGCCTC ACTTTGATGG GATAATGGAT 
GGATTCTCTG GGAAGGCTGC AGAAGACCTC TTCAATGCAC ATGAGATCTT GCCAGGCCCC 
CTCTCTCCAA TGCAGACACA GTTTTCACCC TCTTCTGTGG ACAGCAATGG GCTGCAGTTA 
AGCTTCACTG AATCTCCCTG GGAAACCATG GAGTGGCTGG ACCTCACTCC GCCAAATTCC 
ACACCAGGCT TTAGCGCCCT CACCACCAGC AGCCCCAGCA TCTTCAACAT CGATTTCCTG 
GATGTCACTG ATCTCAATTT GAATTCTTCC ATGGACCTTC ACTTGCAGCA GTGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOCD ( XM_005256863.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256920.1 Homo sapiens myocardin (MYOCD), transcript variant X2, mRNA.

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CloneID	OHu49970
Clone ID Related Accession (Same CDS sequence)	XM_005256863.1
Accession Version	XM_005256863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2832bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myocardin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GGATCTTGCT 
TCTGGCTCAG AAAATGACAG AAATGACTCA GCCTCACAGC CCAGCCACCA GTCAGATGCG 
GGGAAGCAGG GGCTTGGCCC CCCCAGCACC CCCATAGCCG TGCATGCTGC TGTAAAGTCC 
AAATCCTTGG GTGACAGTAA GAACCGCCAC AAAAAGCCCA AGGACCCCAA GCCAAAGGTG 
AAGAAGCTTA AATATCACCA GTACATTCCC CCAGACCAGA AGGCAGAGAA GTCCCCTCCA 
CCTATGGACT CAGCCTACGC TCGGCTGCTC CAGCAACAGC AGCTGTTCCT GCAGCTCCAA 
ATCCTCAGCC AGCAGCAGCA GCAGCAGCAA CACCGATTCA GCTACCTAGG GATGCACCAA 
GCTCAGCTTA AGGAACCAAA TGAACAGATG GTCAGAAATC CAAACTCTTC TTCAACGCCA 
CTGAGCAATA CCCCCTTGTC TCCTGTCAAA AACAGTTTTT CTGGACAAAC TGGTGTCTCT 
TCTTTCAAAC CAGGCCCACT CCCACCTAAC CTGGATGATC TGAAGGTCTC TGAATTAAGA 
CAACAGCTTC GAATTCGGGG CTTGCCTGTG TCAGGCACCA AAACGGCTCT CATGGACCGG 
CTTCGACCCT TCCAGGACTG CTCTGGCAAC CCAGTGCCGA ACTTTGGGGA TATAACGACT 
GTCACTTTTC CTGTCACACC CAACACGCTG CCCAATTACC AGTCTTCCTC TTCTACCAGT 
GCCCTGTCCA ACGGCTTCTA CCACTTTGGC AGCACCAGCT CCAGCCCCCC GATCTCCCCA 
GCCTCCTCTG ACCTGTCAGT CGCTGGGTCC CTGCCGGACA CCTTCAATGA TGCCTCCCCC 
TCCTTCGGCC TGCACCCGTC CCCAGTCCAC GTGTGCACGG AGGAAAGTCT CATGAGCAGC 
CTGAATGGGG GCTCTGTTCC TTCTGAGCTG GATGGGCTGG ACTCCGAGAA GGACAAGATG 
CTGGTGGAGA AGCAGAAGGT GATCAATGAA CTCACCTGGA AACTCCAGCA AGAGCAGAGG 
CAGGTGGAGG AGCTGAGGAT GCAGCTTCAG AAGCAGAAAA GGAATAACTG TTCAGAGAAG 
AAGCCGCTGC CTTTCCTGGC TGCCTCCATC AAGCAGGAAG AGGCTGTCTC CAGCTGTCCT 
TTTGCATCCC AAGTACCTGT GAAAAGACAA AGCAGCAGCT CAGAGTGTCA CCCACCGGCT 
TGTGAAGCTG CTCAACTCCA GCCTCTTGGA AATGCTCATT GTGTGGAGTC CTCAGATCAA 
ACCAATGTAC TTTCTTCCAC ATTTCTCAGC CCCCAGTGTT CCCCTCAGCA TTCACCGCTG 
GGGGCTGTGA AAAGCCCACA GCACATCAGT TTGCCCCCAT CACCCAACAA CCCTCACTTT 
CTGCCCTCAT CCTCCGGGGC CCAGGGAGAA GGGCACAGGG TCTCCTCGCC CATCAGCAGC 
CAGGTGTGCA CTGCACAGAA CTCAGGAGCA CACGATGGCC ATCCTCCAAG CTTCTCTCCC 
CATTCTTCCA GCCTCCACCC GCCCTTCTCT GGAGCCCAAG CAGACAGCAG TCATGGTGCC 
GGGGGAAACC CTTGTCCCAA AAGCCCATGT GTACAGCAAA AGCAAATGAC CCGGAGTCAG 
CAGATGGATG AACTCCTGGA CGTGCTTATT GAAAGCGGAG AAATGCCAGC AGACGCTAGA 
GAGGATCACT CATGTCTTCA AAAAGTCCCA AAGATACCCA GATCTTCCCG AAGTCCAACT 
GCTGTCCTCA CCAAGCCCTC GGCTTCCTTT GAACAAGCCT CTTCAGGCAG CCAGATCCCC 
TTTGATCCCT ATGCCACCGA CAGTGATGAG CATCTTGAAG TCTTATTAAA TTCCCAGAGC 
CCCCTAGGAA AGATGAGTGA TGTCACCCTT CTAAAAATTG GGAGCGAAGA GCCTCACTTT 
GATGGGATAA TGGATGGATT CTCTGGGAAG GCTGCAGAAG ACCTCTTCAA TGCACATGAG 
ATCTTGCCAG GCCCCCTCTC TCCAATGCAG ACACAGTTTT CACCCTCTTC TGTGGACAGC 
AATGGGCTGC AGTTAAGCTT CACTGAATCT CCCTGGGAAA CCATGGAGTG GCTGGACCTC 
ACTCCGCCAA ATTCCACACC AGGCTTTAGC GCCCTCACCA CCAGCAGCCC CAGCATCTTC 
AACATCGATT TCCTGGATGT CACTGATCTC AATTTGAATT CTTCCATGGA CCTTCACTTG 
CAGCAGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256920.1
CDS	300..3131
Translation	MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

Target ORF information:

RefSeq Version	XM_005256863.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myocardin (MYOCD), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256863.1

ORF Insert Sequence:

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GGATCTTGCT 
TCTGGCTCAG AAAATGACAG AAATGACTCA GCCTCACAGC CCAGCCACCA GTCAGATGCG 
GGGAAGCAGG GGCTTGGCCC CCCCAGCACC CCCATAGCCG TGCATGCTGC TGTAAAGTCC 
AAATCCTTGG GTGACAGTAA GAACCGCCAC AAAAAGCCCA AGGACCCCAA GCCAAAGGTG 
AAGAAGCTTA AATATCACCA GTACATTCCC CCAGACCAGA AGGCAGAGAA GTCCCCTCCA 
CCTATGGACT CAGCCTACGC TCGGCTGCTC CAGCAACAGC AGCTGTTCCT GCAGCTCCAA 
ATCCTCAGCC AGCAGCAGCA GCAGCAGCAA CACCGATTCA GCTACCTAGG GATGCACCAA 
GCTCAGCTTA AGGAACCAAA TGAACAGATG GTCAGAAATC CAAACTCTTC TTCAACGCCA 
CTGAGCAATA CCCCCTTGTC TCCTGTCAAA AACAGTTTTT CTGGACAAAC TGGTGTCTCT 
TCTTTCAAAC CAGGCCCACT CCCACCTAAC CTGGATGATC TGAAGGTCTC TGAATTAAGA 
CAACAGCTTC GAATTCGGGG CTTGCCTGTG TCAGGCACCA AAACGGCTCT CATGGACCGG 
CTTCGACCCT TCCAGGACTG CTCTGGCAAC CCAGTGCCGA ACTTTGGGGA TATAACGACT 
GTCACTTTTC CTGTCACACC CAACACGCTG CCCAATTACC AGTCTTCCTC TTCTACCAGT 
GCCCTGTCCA ACGGCTTCTA CCACTTTGGC AGCACCAGCT CCAGCCCCCC GATCTCCCCA 
GCCTCCTCTG ACCTGTCAGT CGCTGGGTCC CTGCCGGACA CCTTCAATGA TGCCTCCCCC 
TCCTTCGGCC TGCACCCGTC CCCAGTCCAC GTGTGCACGG AGGAAAGTCT CATGAGCAGC 
CTGAATGGGG GCTCTGTTCC TTCTGAGCTG GATGGGCTGG ACTCCGAGAA GGACAAGATG 
CTGGTGGAGA AGCAGAAGGT GATCAATGAA CTCACCTGGA AACTCCAGCA AGAGCAGAGG 
CAGGTGGAGG AGCTGAGGAT GCAGCTTCAG AAGCAGAAAA GGAATAACTG TTCAGAGAAG 
AAGCCGCTGC CTTTCCTGGC TGCCTCCATC AAGCAGGAAG AGGCTGTCTC CAGCTGTCCT 
TTTGCATCCC AAGTACCTGT GAAAAGACAA AGCAGCAGCT CAGAGTGTCA CCCACCGGCT 
TGTGAAGCTG CTCAACTCCA GCCTCTTGGA AATGCTCATT GTGTGGAGTC CTCAGATCAA 
ACCAATGTAC TTTCTTCCAC ATTTCTCAGC CCCCAGTGTT CCCCTCAGCA TTCACCGCTG 
GGGGCTGTGA AAAGCCCACA GCACATCAGT TTGCCCCCAT CACCCAACAA CCCTCACTTT 
CTGCCCTCAT CCTCCGGGGC CCAGGGAGAA GGGCACAGGG TCTCCTCGCC CATCAGCAGC 
CAGGTGTGCA CTGCACAGAA CTCAGGAGCA CACGATGGCC ATCCTCCAAG CTTCTCTCCC 
CATTCTTCCA GCCTCCACCC GCCCTTCTCT GGAGCCCAAG CAGACAGCAG TCATGGTGCC 
GGGGGAAACC CTTGTCCCAA AAGCCCATGT GTACAGCAAA AGCAAATGAC CCGGAGTCAG 
CAGATGGATG AACTCCTGGA CGTGCTTATT GAAAGCGGAG AAATGCCAGC AGACGCTAGA 
GAGGATCACT CATGTCTTCA AAAAGTCCCA AAGATACCCA GATCTTCCCG AAGTCCAACT 
GCTGTCCTCA CCAAGCCCTC GGCTTCCTTT GAACAAGCCT CTTCAGGCAG CCAGATCCCC 
TTTGATCCCT ATGCCACCGA CAGTGATGAG CATCTTGAAG TCTTATTAAA TTCCCAGAGC 
CCCCTAGGAA AGATGAGTGA TGTCACCCTT CTAAAAATTG GGAGCGAAGA GCCTCACTTT 
GATGGGATAA TGGATGGATT CTCTGGGAAG GCTGCAGAAG ACCTCTTCAA TGCACATGAG 
ATCTTGCCAG GCCCCCTCTC TCCAATGCAG ACACAGTTTT CACCCTCTTC TGTGGACAGC 
AATGGGCTGC AGTTAAGCTT CACTGAATCT CCCTGGGAAA CCATGGAGTG GCTGGACCTC 
ACTCCGCCAA ATTCCACACC AGGCTTTAGC GCCCTCACCA CCAGCAGCCC CAGCATCTTC 
AACATCGATT TCCTGGATGT CACTGATCTC AATTTGAATT CTTCCATGGA CCTTCACTTG 
CAGCAGTGGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYOCD ( XM_017025342.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880831.1 Homo sapiens myocardin (MYOCD), transcript variant X1, mRNA.

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CloneID	OHu87645
Clone ID Related Accession (Same CDS sequence)	XM_017025342.1
Accession Version	XM_017025342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2835bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myocardin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GTCCAAATCC 
TTGGGTGACA GTAAGAACCG CCACAAAAAG CCCAAGGACC CCAAGCCAAA GGTGAAGAAG 
CTTAAATATC ACCAGTACAT TCCCCCAGAC CAGAAGGCAG AGAAGTCCCC TCCACCTATG 
GACTCAGCCT ACGCTCGGCT GCTCCAGCAA CAGCAGCTGT TCCTGCAGCT CCAAATCCTC 
AGCCAGCAGC AGCAGCAGCA GCAACACCGA TTCAGCTACC TAGGGATGCA CCAAGCTCAG 
CTTAAGGAAC CAAATGAACA GATGGTCAGA AATCCAAACT CTTCTTCAAC GCCACTGAGC 
AATACCCCCT TGTCTCCTGT CAAAAACAGT TTTTCTGGAC AAACTGGTGT CTCTTCTTTC 
AAACCAGGCC CACTCCCACC TAACCTGGAT GATCTGAAGG TCTCTGAATT AAGACAACAG 
CTTCGAATTC GGGGCTTGCC TGTGTCAGGC ACCAAAACGG CTCTCATGGA CCGGCTTCGA 
CCCTTCCAGG ACTGCTCTGG CAACCCAGTG CCGAACTTTG GGGATATAAC GACTGTCACT 
TTTCCTGTCA CACCCAACAC GCTGCCCAAT TACCAGTCTT CCTCTTCTAC CAGTGCCCTG 
TCCAACGGCT TCTACCACTT TGGCAGCACC AGCTCCAGCC CCCCGATCTC CCCAGCCTCC 
TCTGACCTGT CAGTCGCTGG GTCCCTGCCG GACACCTTCA ATGATGCCTC CCCCTCCTTC 
GGCCTGCACC CGTCCCCAGT CCACGTGTGC ACGGAGGAAA GTCTCATGAG CAGCCTGAAT 
GGGGGCTCTG TTCCTTCTGA GCTGGATGGG CTGGACTCCG AGAAGGACAA GATGCTGGTG 
GAGAAGCAGA AGGTGATCAA TGAACTCACC TGGAAACTCC AGCAAGAGCA GAGGCAGGTG 
GAGGAGCTGA GGATGCAGCT TCAGAAGCAG AAAAGGAATA ACTGTTCAGA GAAGAAGCCG 
CTGCCTTTCC TGGCTGCCTC CATCAAGCAG GAAGAGGCTG TCTCCAGCTG TCCTTTTGCA 
TCCCAAGTAC CTGTGAAAAG ACAAAGCAGC AGCTCAGAGT GTCACCCACC GGCTTGTGAA 
GCTGCTCAAC TCCAGCCTCT TGGAAATGCT CATTGTGTGG AGTCCTCAGA TCAAACCAAT 
GTACTTTCTT CCACATTTCT CAGCCCCCAG TGTTCCCCTC AGCATTCACC GCTGGGGGCT 
GTGAAAAGCC CACAGCACAT CAGTTTGCCC CCATCACCCA ACAACCCTCA CTTTCTGCCC 
TCATCCTCCG GGGCCCAGGG AGAAGGGCAC AGGGTCTCCT CGCCCATCAG CAGCCAGGTG 
TGCACTGCAC AGAACTCAGG AGCACACGAT GGCCATCCTC CAAGCTTCTC TCCCCATTCT 
TCCAGCCTCC ACCCGCCCTT CTCTGGAGCC CAAGCAGACA GCAGTCATGG TGCCGGGGGA 
AACCCTTGTC CCAAAAGCCC ATGTGTACAG CAAAAGATGG CTGGTTTACA CTCTTCTGAT 
AAGGTGGGGC CAAAGTTTTC AATTCCATCC CCAACTTTTT CTAAGTCAAG TTCAGCAATT 
TCAGAGGTAA CACAGCCTCC ATCCTATGAA GATGCCGTAA AGCAGCAAAT GACCCGGAGT 
CAGCAGATGG ATGAACTCCT GGACGTGCTT ATTGAAAGCG GAGAAATGCC AGCAGACGCT 
AGAGAGGATC ACTCATGTCT TCAAAAAGTC CCAAAGATAC CCAGATCTTC CCGAAGTCCA 
ACTGCTGTCC TCACCAAGCC CTCGGCTTCC TTTGAACAAG CCTCTTCAGG CAGCCAGATC 
CCCTTTGATC CCTATGCCAC CGACAGTGAT GAGCATCTTG AAGTCTTATT AAATTCCCAG 
AGCCCCCTAG GAAAGATGAG TGATGTCACC CTTCTAAAAA TTGGGAGCGA AGAGCCTCAC 
TTTGATGGGA TAATGGATGG ATTCTCTGGG AAGGCTGCAG AAGACCTCTT CAATGCACAT 
GAGATCTTGC CAGGCCCCCT CTCTCCAATG CAGACACAGT TTTCACCCTC TTCTGTGGAC 
AGCAATGGGC TGCAGTTAAG CTTCACTGAA TCTCCCTGGG AAACCATGGA GTGGCTGGAC 
CTCACTCCGC CAAATTCCAC ACCAGGCTTT AGCGCCCTCA CCACCAGCAG CCCCAGCATC 
TTCAACATCG ATTTCCTGGA TGTCACTGAT CTCAATTTGA ATTCTTCCAT GGACCTTCAC 
TTGCAGCAGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880831.1
CDS	300..3134
Translation	MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQNSGAHDGHPPSFSPHSSSLHPPFSGAQADSSHGAGGNPCPKSPCVQQKMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

Target ORF information:

RefSeq Version	XM_017025342.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myocardin (MYOCD), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025342.1

ORF Insert Sequence:

ATGACACTCC TGGGGTCTGA GCATTCCTTG CTGATTAGGA GCAAGTTCAG ATCAGTTTTA 
CAGTTAAGAC TTCAACAAAG AAGGACCCAG GAACAACTGG CTAACCAAGG CATAATACCA 
CCACTGAAAC GTCCAGCTGA ATTCCATGAG CAAAGAAAAC ATTTGGATAG TGACAAGGCT 
AAAAATTCCC TGAAGCGCAA AGCCAGAAAC AGGTGCAACA GTGCCGACTT GGTTAATATG 
CACATACTCC AAGCTTCCAC TGCAGAGAGG TCCATTCCAA CTGCTCAGAT GAAGCTGAAA 
AGAGCCCGAC TCGCCGATGA TCTCAATGAA AAAATTGCTC TACGACCAGG GCCACTGGAG 
CTGGTGGAAA AAAACATTCT TCCTGTGGAT TCTGCTGTGA AAGAGGCCAT AAAAGGTAAC 
CAGGTGAGTT TCTCCAAATC CACGGATGCT TTTGCCTTTG AAGAGGACAG CAGCAGCGAT 
GGGCTTTCTC CGGATCAGAC TCGAAGTGAA GACCCCCAAA ACTCAGCGGG ATCCCCGCCA 
GACGCTAAAG CCTCAGATAC CCCTTCGACA GGTTCTCTGG GGACAAACCA GTCCAAATCC 
TTGGGTGACA GTAAGAACCG CCACAAAAAG CCCAAGGACC CCAAGCCAAA GGTGAAGAAG 
CTTAAATATC ACCAGTACAT TCCCCCAGAC CAGAAGGCAG AGAAGTCCCC TCCACCTATG 
GACTCAGCCT ACGCTCGGCT GCTCCAGCAA CAGCAGCTGT TCCTGCAGCT CCAAATCCTC 
AGCCAGCAGC AGCAGCAGCA GCAACACCGA TTCAGCTACC TAGGGATGCA CCAAGCTCAG 
CTTAAGGAAC CAAATGAACA GATGGTCAGA AATCCAAACT CTTCTTCAAC GCCACTGAGC 
AATACCCCCT TGTCTCCTGT CAAAAACAGT TTTTCTGGAC AAACTGGTGT CTCTTCTTTC 
AAACCAGGCC CACTCCCACC TAACCTGGAT GATCTGAAGG TCTCTGAATT AAGACAACAG 
CTTCGAATTC GGGGCTTGCC TGTGTCAGGC ACCAAAACGG CTCTCATGGA CCGGCTTCGA 
CCCTTCCAGG ACTGCTCTGG CAACCCAGTG CCGAACTTTG GGGATATAAC GACTGTCACT 
TTTCCTGTCA CACCCAACAC GCTGCCCAAT TACCAGTCTT CCTCTTCTAC CAGTGCCCTG 
TCCAACGGCT TCTACCACTT TGGCAGCACC AGCTCCAGCC CCCCGATCTC CCCAGCCTCC 
TCTGACCTGT CAGTCGCTGG GTCCCTGCCG GACACCTTCA ATGATGCCTC CCCCTCCTTC 
GGCCTGCACC CGTCCCCAGT CCACGTGTGC ACGGAGGAAA GTCTCATGAG CAGCCTGAAT 
GGGGGCTCTG TTCCTTCTGA GCTGGATGGG CTGGACTCCG AGAAGGACAA GATGCTGGTG 
GAGAAGCAGA AGGTGATCAA TGAACTCACC TGGAAACTCC AGCAAGAGCA GAGGCAGGTG 
GAGGAGCTGA GGATGCAGCT TCAGAAGCAG AAAAGGAATA ACTGTTCAGA GAAGAAGCCG 
CTGCCTTTCC TGGCTGCCTC CATCAAGCAG GAAGAGGCTG TCTCCAGCTG TCCTTTTGCA 
TCCCAAGTAC CTGTGAAAAG ACAAAGCAGC AGCTCAGAGT GTCACCCACC GGCTTGTGAA 
GCTGCTCAAC TCCAGCCTCT TGGAAATGCT CATTGTGTGG AGTCCTCAGA TCAAACCAAT 
GTACTTTCTT CCACATTTCT CAGCCCCCAG TGTTCCCCTC AGCATTCACC GCTGGGGGCT 
GTGAAAAGCC CACAGCACAT CAGTTTGCCC CCATCACCCA ACAACCCTCA CTTTCTGCCC 
TCATCCTCCG GGGCCCAGGG AGAAGGGCAC AGGGTCTCCT CGCCCATCAG CAGCCAGGTG 
TGCACTGCAC AGAACTCAGG AGCACACGAT GGCCATCCTC CAAGCTTCTC TCCCCATTCT 
TCCAGCCTCC ACCCGCCCTT CTCTGGAGCC CAAGCAGACA GCAGTCATGG TGCCGGGGGA 
AACCCTTGTC CCAAAAGCCC ATGTGTACAG CAAAAGATGG CTGGTTTACA CTCTTCTGAT 
AAGGTGGGGC CAAAGTTTTC AATTCCATCC CCAACTTTTT CTAAGTCAAG TTCAGCAATT 
TCAGAGGTAA CACAGCCTCC ATCCTATGAA GATGCCGTAA AGCAGCAAAT GACCCGGAGT 
CAGCAGATGG ATGAACTCCT GGACGTGCTT ATTGAAAGCG GAGAAATGCC AGCAGACGCT 
AGAGAGGATC ACTCATGTCT TCAAAAAGTC CCAAAGATAC CCAGATCTTC CCGAAGTCCA 
ACTGCTGTCC TCACCAAGCC CTCGGCTTCC TTTGAACAAG CCTCTTCAGG CAGCCAGATC 
CCCTTTGATC CCTATGCCAC CGACAGTGAT GAGCATCTTG AAGTCTTATT AAATTCCCAG 
AGCCCCCTAG GAAAGATGAG TGATGTCACC CTTCTAAAAA TTGGGAGCGA AGAGCCTCAC 
TTTGATGGGA TAATGGATGG ATTCTCTGGG AAGGCTGCAG AAGACCTCTT CAATGCACAT 
GAGATCTTGC CAGGCCCCCT CTCTCCAATG CAGACACAGT TTTCACCCTC TTCTGTGGAC 
AGCAATGGGC TGCAGTTAAG CTTCACTGAA TCTCCCTGGG AAACCATGGA GTGGCTGGAC 
CTCACTCCGC CAAATTCCAC ACCAGGCTTT AGCGCCCTCA CCACCAGCAG CCCCAGCATC 
TTCAACATCG ATTTCCTGGA TGTCACTGAT CTCAATTTGA ATTCTTCCAT GGACCTTCAC 
TTGCAGCAGT GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of cardiac myocyte cell death and differentiation by myocardin.
Molecular and cellular biochemistry437(1-2)119-131(2018 Jan)
Gordon JW

Myocardin inhibited the gap protein connexin 43 via promoted miR-206 to regulate vascular smooth muscle cell phenotypic switch.
Gene61622-30(2017 Jun)
Li H,Xiang Y,Fan LJ,Zhang XY,Li JP,Yu CX,Bao LY,Cao DS,Xing WB,Liao XH,Zhang TC

VEGF-A Stimulates STAT3 Activity via Nitrosylation of Myocardin to Regulate the Expression of Vascular Smooth Muscle Cell Differentiation Markers.
Scientific reports7(1)2660(2017 Jun)
Liao XH,Xiang Y,Li H,Zheng L,Xu Y,Xi Yu C,Li JP,Zhang XY,Xing WB,Cao DS,Bao LY,Zhang TC

Yes-Associated Protein Inhibits Transcription of Myocardin and Attenuates Differentiation of Vascular Smooth Muscle Cell from Cardiovascular Progenitor Cell Lineage.
Stem cells (Dayton, Ohio)35(2)351-361(2017 Feb)
Wang L,Qiu P,Jiao J,Hirai H,Xiong W,Zhang J,Zhu T,Ma PX,Chen YE,Yang B

In search of novel targets for heart disease: myocardin and myocardin-related transcriptional cofactors.
Biochemistry research international2012973723(2012)
Mikhailov AT,Torrado M

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MYOCD cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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