MRFAP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRFAP1
Entrez Gene ID	93621
Full Name	Morf4 family associated protein 1
Synonyms	PAM14,PGR1
General protein information	Preferred Names Morf4 family associated protein 1 Names MORF4 family-associated protein 1 Mof4 family associated protein 1 T-cell activation protein protein associated with MRG of 14 kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.1
Summary	This gene encodes an intracellular protein that interacts with members of the MORF4/MRG (mortality factor on chromosome 4/MORF4 related gene) family and the tumor suppressor Rb (retinoblastoma protein.) The protein may play a role in senescence, cell growth and immortalization. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013].
Disorder MIM:	616905

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272054.1	NP_001258983.1	MORF4 family-associated protein 1 isoform b
NM_033296.2	NP_150638.1	MORF4 family-associated protein 1 isoform a
NM_001272053.1	NP_001258982.1	MORF4 family-associated protein 1 isoform a

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MRFAP1	XP_005618499.1
Bos taurus (cattle)	MRFAP1	NP_001030421.1
Rattus norvegicus (Norway rat)	Mrfap1	NP_001009264.1
	MRFAP1	XP_001092113.1
Mus musculus (house mouse)	Mrfap1	NP_080518.1
Homo sapiens (human)	MRFAP1	NP_150638.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

A Y2H-seq approach defines the human protein methyltransferase interactome.
Weimann M, Grossmann A, Woodsmith J, ?zkan Z, Birth P, Meierhofer D, Benlasfer N, Valovka T, Timmermann B, Wanker EE, Sauer S, Stelzl U
Nature methods10(4)339-42(2013 Apr)

Characterization of MRFAP1 turnover and interactions downstream of the NEDD8 pathway.
Larance M, Kirkwood KJ, Xirodimas DP, Lundberg E, Uhlen M, Lamond AI
Molecular & cellular proteomics : MCP11(3)M111.014407(2012 Mar)

Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.
Liao H, Liu XJ, Blank JL, Bouck DC, Bernard H, Garcia K, Lightcap ES
Molecular & cellular proteomics : MCP10(11)M111.009183(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The results suggest that nucleus-localized Sedlin may play a role in regulation of transcriptional activities of the MRG family of transcription factors via binding to PAM14.
Title: Interaction of Sedlin with PAM14.

identify MRG15 residues that form a shallow hydrophobic pocket to interact with the N-terminal 50 residues of PAM14 through primarily hydrophobic interactions.
Title: The MRG domain of human MRG15 uses a shallow hydrophobic pocket to interact with the N-terminal region of PAM14.

The following MRFAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRFAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00592	NM_001272053.1 Latest version!	Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00592	NM_033296.2 Latest version!	Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu00512	NM_001272054.1 Latest version!	Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRFAP1 ( NM_033296.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_150638.1 Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00592
Clone ID Related Accession (Same CDS sequence)	NM_033296.2 , NM_001272053.1
Accession Version	NM_033296.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	MORF4 family-associated protein 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK123360.1, DA253671.1, AK074496.1, BG255189.1, AA486335.1 and AL539167.3. On Jan 5, 2013 this sequence version replaced NM_033296.1. Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279842.4819.1, SRR1803612.87542.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGGACATCGT CGAGCTGGCG GAACCGGAGG AAGTGGAGGT GCTGGAGCCC 
GAGGAGGATT TCGAGCAGTT TCTGCTCCCG GTCATCAACG AGATGCGCGA GGACATCGCG 
TCGCTGACGC GCGAGCACGG GCGGGCGTAC CTGCGGAACC GGAGCAAGCT GTGGGAGATG 
GACAATATGC TCATCCAGAT CAAAACGCAG GTGGAGGCCT CGGAGGAGAG CGCCCTCAAC 
CACCTCCAGA ACCCGGGCGA CGCGGCCGAG GGCCGGGCGG CCAAGAGGTG CGAGAAGGCC 
GAGGAGAAGG CCAAGGAGAT TGCGAAGATG GCAGAGATGC TGGTGGAGCT GGTCCGGCGG 
ATAGAGAAGA GCGAGTCGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_150638.1
CDS	773..1156
Translation	MRPLDIVELAEPEEVEVLEPEEDFEQFLLPVINEMREDIASLTREHGRAYLRNRSKLWEMDNMLIQIKTQVEASEESALNHLQNPGDAAEGRAAKRCEKAEEKAKEIAKMAEMLVELVRRIEKSESS

Target ORF information:

RefSeq Version	NM_033296.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033296.2

ORF Insert Sequence:

ATGCGGCCCC TGGACATCGT CGAGCTGGCG GAACCGGAGG AAGTGGAGGT GCTGGAGCCC 
GAGGAGGATT TCGAGCAGTT TCTGCTCCCG GTCATCAACG AGATGCGCGA GGACATCGCG 
TCGCTGACGC GCGAGCACGG GCGGGCGTAC CTGCGGAACC GGAGCAAGCT GTGGGAGATG 
GACAATATGC TCATCCAGAT CAAAACGCAG GTGGAGGCCT CGGAGGAGAG CGCCCTCAAC 
CACCTCCAGA ACCCGGGCGA CGCGGCCGAG GGCCGGGCGG CCAAGAGGTG CGAGAAGGCC 
GAGGAGAAGG CCAAGGAGAT TGCGAAGATG GCAGAGATGC TGGTGGAGCT GGTCCGGCGG 
ATAGAGAAGA GCGAGTCGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRFAP1 ( NM_001272053.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258982.1 Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu00592
Clone ID Related Accession (Same CDS sequence)	NM_033296.2 , NM_001272053.1
Accession Version	NM_001272053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	MORF4 family-associated protein 1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK123360.1, BG255189.1, AA486335.1 and AL539167.3. Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK123360.1, SRR1660809.179685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGGACATCGT CGAGCTGGCG GAACCGGAGG AAGTGGAGGT GCTGGAGCCC 
GAGGAGGATT TCGAGCAGTT TCTGCTCCCG GTCATCAACG AGATGCGCGA GGACATCGCG 
TCGCTGACGC GCGAGCACGG GCGGGCGTAC CTGCGGAACC GGAGCAAGCT GTGGGAGATG 
GACAATATGC TCATCCAGAT CAAAACGCAG GTGGAGGCCT CGGAGGAGAG CGCCCTCAAC 
CACCTCCAGA ACCCGGGCGA CGCGGCCGAG GGCCGGGCGG CCAAGAGGTG CGAGAAGGCC 
GAGGAGAAGG CCAAGGAGAT TGCGAAGATG GCAGAGATGC TGGTGGAGCT GGTCCGGCGG 
ATAGAGAAGA GCGAGTCGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258982.1
CDS	654..1037
Translation	MRPLDIVELAEPEEVEVLEPEEDFEQFLLPVINEMREDIASLTREHGRAYLRNRSKLWEMDNMLIQIKTQVEASEESALNHLQNPGDAAEGRAAKRCEKAEEKAKEIAKMAEMLVELVRRIEKSESS

Target ORF information:

RefSeq Version	NM_001272053.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272053.1

ORF Insert Sequence:

ATGCGGCCCC TGGACATCGT CGAGCTGGCG GAACCGGAGG AAGTGGAGGT GCTGGAGCCC 
GAGGAGGATT TCGAGCAGTT TCTGCTCCCG GTCATCAACG AGATGCGCGA GGACATCGCG 
TCGCTGACGC GCGAGCACGG GCGGGCGTAC CTGCGGAACC GGAGCAAGCT GTGGGAGATG 
GACAATATGC TCATCCAGAT CAAAACGCAG GTGGAGGCCT CGGAGGAGAG CGCCCTCAAC 
CACCTCCAGA ACCCGGGCGA CGCGGCCGAG GGCCGGGCGG CCAAGAGGTG CGAGAAGGCC 
GAGGAGAAGG CCAAGGAGAT TGCGAAGATG GCAGAGATGC TGGTGGAGCT GGTCCGGCGG 
ATAGAGAAGA GCGAGTCGTC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRFAP1 ( NM_001272054.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258983.1 Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu00512
Clone ID Related Accession (Same CDS sequence)	NM_001272054.1
Accession Version	NM_001272054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	MORF4 family-associated protein 1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA253671.1, HY038123.1, BP309093.1, AK074496.1, CB052459.1, AF090922.1, BI858568.1, AA486335.1 and AL539167.3. Transcript Variant: This variant (3) uses an alternate splice site in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP309093.1, BP309119.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCCCC TGGACATCGT CGAGCTGGCG GAACCGGAGG AAGTGGAGGT GCTGGAGCCC 
GAGGAGGATT TCGAGCAGTT TCTGCTCCCG GTCATCAACG AGATGCGCGA GGACATCGCG 
TCGCTGACGC GCGAGCACGG GCGGGCGTAC CTGCGGAACC GGAGCAAGCT GTGGGAGATG 
GACAATATGC TCATCCAGAT CAAAACGCAG GTGGAGGCCT CGGAGGAGAG CGCCCTCAAC 
CACCTCCAGA ACCCGGGCGA CGCGGCCGAG GGCCGGGCGG CCAAGAGGTG CGAGAAGAGC 
GAGTCGTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258983.1
CDS	773..1084
Translation	MRPLDIVELAEPEEVEVLEPEEDFEQFLLPVINEMREDIASLTREHGRAYLRNRSKLWEMDNMLIQIKTQVEASEESALNHLQNPGDAAEGRAAKRCEKSESS

Target ORF information:

RefSeq Version	NM_001272054.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Morf4 family associated protein 1 (MRFAP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272054.1

ORF Insert Sequence:

ATGCGGCCCC TGGACATCGT CGAGCTGGCG GAACCGGAGG AAGTGGAGGT GCTGGAGCCC 
GAGGAGGATT TCGAGCAGTT TCTGCTCCCG GTCATCAACG AGATGCGCGA GGACATCGCG 
TCGCTGACGC GCGAGCACGG GCGGGCGTAC CTGCGGAACC GGAGCAAGCT GTGGGAGATG 
GACAATATGC TCATCCAGAT CAAAACGCAG GTGGAGGCCT CGGAGGAGAG CGCCCTCAAC 
CACCTCCAGA ACCCGGGCGA CGCGGCCGAG GGCCGGGCGG CCAAGAGGTG CGAGAAGAGC 
GAGTCGTCGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

A Y2H-seq approach defines the human protein methyltransferase interactome.
Nature methods10(4)339-42(2013 Apr)
Weimann M,Grossmann A,Woodsmith J,?zkan Z,Birth P,Meierhofer D,Benlasfer N,Valovka T,Timmermann B,Wanker EE,Sauer S,Stelzl U

Characterization of MRFAP1 turnover and interactions downstream of the NEDD8 pathway.
Molecular & cellular proteomics : MCP11(3)M111.014407(2012 Mar)
Larance M,Kirkwood KJ,Xirodimas DP,Lundberg E,Uhlen M,Lamond AI

Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.
Molecular & cellular proteomics : MCP10(11)M111.009183(2011 Nov)
Liao H,Liu XJ,Blank JL,Bouck DC,Bernard H,Garcia K,Lightcap ES

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MRFAP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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