LONP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LONP1
Entrez Gene ID	9361
Full Name	lon peptidase 1, mitochondrial
Synonyms	CODASS,LON,LONP,LonHS,PIM1,PRSS15,hLON
General protein information	Preferred Names lon peptidase 1, mitochondrial Names lon protease homolog, mitochondrial hLON ATP-dependent protease lon protease-like protein mitochondrial ATP-dependent protease Lon mitochondrial lon protease-like protein serine protease 15
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.3
Summary	This gene encodes a mitochondrial matrix protein that belongs to the Lon family of ATP-dependent proteases. This protein mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides in the mitochondrial matrix. It may also have a chaperone function in the assembly of inner membrane protein complexes, and participate in the regulation of mitochondrial gene expression and maintenance of the integrity of the mitochondrial genome. Decreased expression of this gene has been noted in a patient with hereditary spastic paraplegia (PMID:18378094). Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013].
Disorder MIM:	605490
Disorder Html:	CODAS syndrome, 600373 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528441.2	XP_011526743.1	lon protease homolog, mitochondrial isoform X1
NM_001276480.1	NP_001263409.1	lon protease homolog, mitochondrial isoform 3
NM_001276479.1	NP_001263408.1	lon protease homolog, mitochondrial isoform 2
XM_011528441.3	XP_011526743.1	lon protease homolog, mitochondrial isoform X1
NM_004793.4	NP_004784.2	lon protease homolog, mitochondrial isoform 1 precursor
NM_001276479.2	NP_001263408.1	lon protease homolog, mitochondrial isoform 2
NM_004793.3	NP_004784.2	lon protease homolog, mitochondrial isoform 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	LONP1	XP_001143873.1
Canis lupus familiaris (dog)	LONP1	XP_854391.2
Mus musculus (house mouse)	Lonp1	NP_083058.2
Rattus norvegicus (Norway rat)	Lonp1	NP_596895.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010451	XP_311497.4
Caenorhabditis elegans (roundworm)	C34B2.6	NP_492796.1
Homo sapiens (human)	LONP1	NP_004784.2
Bos taurus (cattle)	LONP1	NP_001015569.2
Danio rerio (zebrafish)	lonp1	XP_691712.4
Saccharomyces cerevisiae (baker's yeast)	PIM1	NP_009531.1
Kluyveromyces lactis	KLLA0E10407g	XP_454420.1
Magnaporthe oryzae (rice blast fungus)	MGG_11141	XP_003714792.1
Xenopus tropicalis (tropical clawed frog)	LOC100492505	XP_002938599.1
	LONP1	XP_001088663.2
Gallus gallus (chicken)	LONP1	XP_001232112.2
Oryza sativa (rice)	Os07g0689300	NP_001175344.1
Schizosaccharomyces pombe (fission yeast)	lon1	NP_593035.1
Drosophila melanogaster (fruit fly)	Lon	NP_649133.1
Neurospora crassa	NCU05261	XP_961826.1
Arabidopsis thaliana (thale cress)	LON1	NP_568490.3

Gene Ontology

The following LONP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LONP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10021	NM_001276480.1 Latest version!	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu20290	NM_004793.4 Latest version!	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$469.50-$657.30 $939.00
OHu10182	NM_001276479.2 Latest version!	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu62723	XM_011528441.3 Latest version!	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu62723	XM_011528441.2	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu10182	NM_001276479.1	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu20290	NM_004793.3	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$469.50-$657.30 $939.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LONP1 ( NM_001276480.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263409.1 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 3, mRNA.

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CloneID	OHu10021
Clone ID Related Accession (Same CDS sequence)	NM_001276480.1
Accession Version	NM_001276480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056366.1 and BC000235.1. Transcript Variant: This variant (3) contains an alternate 5' terminal exon and uses an in-frame downstream start codon compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056366.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGGACC TTGGGGACAA GCTGCGCATG ATCGTCATGG GACACAGAAG AGTCCATATC 
AGCAGACAGC TGGAGGTGGA GCCCGAGGAG CCGGAGGCGG AGAACAAGCA CAAGCCCCGC 
AGGAAGTCAA AGCGGGGCAA GAAGGAGGCG GAGGACGAGC TGAGCGCCAG GCACCCGGCG 
GAGCTGGCGA TGGAGCCCAC CCCTGAGCTC CCGGCTGAGG TGCTCATGGT GGAGGTAGAG 
AACGTTGTCC ACGAGGACTT CCAGGTCACG GAGGAGGTGA AAGCCCTGAC TGCAGAGATC 
GTGAAGACCA TCCGGGACAT CATTGCCTTG AACCCTCTCT ACAGGGAGTC AGTGCTGCAG 
ATGATGCAGG CTGGCCAGCG GGTGGTGGAC AACCCCATCT ACCTGAGCGA CATGGGCGCC 
GCGCTCACCG GGGCCGAGTC CCATGAGCTG CAGGACGTCC TGGAAGAGAC CAATATTCCT 
AAGCGGCTGT ACAAGGCCCT CTCCCTGCTG AAGAAGGAAT TTGAACTGAG CAAGCTGCAG 
CAGCGCCTGG GGCGGGAGGT GGAGGAGAAG ATCAAGCAGA CCCACCGTAA GTACCTGCTG 
CAGGAGCAGC TAAAGATCAT CAAGAAGGAG CTGGGCCTGG AGAAGGACGA CAAGGATGCC 
ATCGAGGAGA AGTTCCGGGA GCGCCTGAAG GAGCTCGTGG TCCCCAAGCA CGTCATGGAT 
GTTGTGGACG AGGAGCTGAG CAAGCTGGGC CTGCTGGACA ACCACTCCTC GGAGTTCAAT 
GTCACCCGCA ACTACCTAGA CTGGCTCACG TCCATCCCTT GGGGCAAGTA CAGCAACGAG 
AACCTGGACC TGGCGCGGGC ACAGGCAGTG CTGGAGGAAG ACCACTACGG CATGGAGGAC 
GTCAAGAAAC GCATCCTGGA GTTCATTGCC GTTAGCCAGC TCCGCGGCTC CACCCAGGGC 
AAGATCCTCT GCTTCTATGG CCCCCCTGGC GTGGGTAAGA CCAGCATTGC TCGCTCCATC 
GCCCGCGCCC TGAACCGAGA GTACTTCCGC TTCAGCGTCG GGGGCATGAC TGACGTGGCT 
GAGATCAAGG GCCACAGGCG GACCTACGTG GGCGCCATGC CCGGGAAGAT CATCCAGTGT 
TTGAAGAAGA CCAAGACGGA GAACCCCCTG ATCCTCATCG ACGAGGTGGA CAAGATCGGC 
CGAGGCTACC AGGGGGACCC GTCGTCGGCA CTGCTGGAGC TGCTGGACCC AGAGCAGAAT 
GCCAACTTCC TGGACCACTA CCTGGACGTG CCCGTGGACT TGTCCAAGGT GCTGTTCATC 
TGCACGGCCA ACGTCACGGA CACCATCCCC GAGCCGCTGC GAGACCGTAT GGAGATGATC 
AACGTGTCGG GCTACGTGGC CCAGGAGAAG CTGGCCATTG CGGAGCGCTA CCTGGTGCCC 
CAGGCTCGCG CCCTGTGTGG CTTGGATGAG AGCAAGGCCA AGCTGTCATC GGACGTGCTG 
ACGCTGCTCA TCAAGCAGTA CTGCCGCGAG AGCGGTGTCC GCAACCTGCA GAAGCAAGTG 
GAGAAGGTGT TACGGAAATC GGCCTACAAG ATTGTCAGCG GCGAGGCCGA GTCCGTGGAG 
GTGACGCCCG AGAACCTGCA GGACTTCGTG GGGAAGCCCG TGTTCACCGT GGAGCGCATG 
TATGACGTGA CACCGCCCGG CGTGGTCATG GGGCTGGCCT GGACCGCAAT GGGAGGCTCC 
ACGCTGTTTG TGGAGACATC CCTGAGACGG CCACAGGACA AGGATGCCAA GGGTGACAAG 
GATGGCAGCC TGGAGGTGAC AGGCCAGCTG GGGGAGGTGA TGAAGGAGAG CGCCCGCATA 
GCCTACACCT TCGCCAGAGC CTTCCTCATG CAGCACGCCC CCGCCAATGA CTACCTGGTG 
ACCTCACACA TCCACCTGCA TGTGCCCGAG GGCGCCACCC CCAAGGACGG CCCAAGCGCA 
GGCTGCACCA TCGTCACGGC CCTGCTGTCC CTGGCCATGG GCAGGCCTGT CCGGCAGAAT 
CTGGCCATGA CTGGCGAAGT CTCCCTCACG GGCAAGATCC TGCCTGTTGG TGGCATCAAG 
GAGAAGACCA TTGCGGCCAA GCGCGCAGGG GTGACGTGCA TCGTCCTGCC AGCCGAGAAC 
AAGAAGGACT TCTACGACCT GGCAGCCTTC ATCACCGAGG GCCTGGAGGT GCACTTCGTG 
GAACACTACC GGGAGATCTT CGACATCGCC TTCCCGGACG AGCAGGCAGA GGCGCTGGCC 
GTGGAACGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263409.1
CDS	394..2685
Translation	MQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

Target ORF information:

RefSeq Version	NM_001276480.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276480.1

ORF Insert Sequence:

ATGCAGGACC TTGGGGACAA GCTGCGCATG ATCGTCATGG GACACAGAAG AGTCCATATC 
AGCAGACAGC TGGAGGTGGA GCCCGAGGAG CCGGAGGCGG AGAACAAGCA CAAGCCCCGC 
AGGAAGTCAA AGCGGGGCAA GAAGGAGGCG GAGGACGAGC TGAGCGCCAG GCACCCGGCG 
GAGCTGGCGA TGGAGCCCAC CCCTGAGCTC CCGGCTGAGG TGCTCATGGT GGAGGTAGAG 
AACGTTGTCC ACGAGGACTT CCAGGTCACG GAGGAGGTGA AAGCCCTGAC TGCAGAGATC 
GTGAAGACCA TCCGGGACAT CATTGCCTTG AACCCTCTCT ACAGGGAGTC AGTGCTGCAG 
ATGATGCAGG CTGGCCAGCG GGTGGTGGAC AACCCCATCT ACCTGAGCGA CATGGGCGCC 
GCGCTCACCG GGGCCGAGTC CCATGAGCTG CAGGACGTCC TGGAAGAGAC CAATATTCCT 
AAGCGGCTGT ACAAGGCCCT CTCCCTGCTG AAGAAGGAAT TTGAACTGAG CAAGCTGCAG 
CAGCGCCTGG GGCGGGAGGT GGAGGAGAAG ATCAAGCAGA CCCACCGTAA GTACCTGCTG 
CAGGAGCAGC TAAAGATCAT CAAGAAGGAG CTGGGCCTGG AGAAGGACGA CAAGGATGCC 
ATCGAGGAGA AGTTCCGGGA GCGCCTGAAG GAGCTCGTGG TCCCCAAGCA CGTCATGGAT 
GTTGTGGACG AGGAGCTGAG CAAGCTGGGC CTGCTGGACA ACCACTCCTC GGAGTTCAAT 
GTCACCCGCA ACTACCTAGA CTGGCTCACG TCCATCCCTT GGGGCAAGTA CAGCAACGAG 
AACCTGGACC TGGCGCGGGC ACAGGCAGTG CTGGAGGAAG ACCACTACGG CATGGAGGAC 
GTCAAGAAAC GCATCCTGGA GTTCATTGCC GTTAGCCAGC TCCGCGGCTC CACCCAGGGC 
AAGATCCTCT GCTTCTATGG CCCCCCTGGC GTGGGTAAGA CCAGCATTGC TCGCTCCATC 
GCCCGCGCCC TGAACCGAGA GTACTTCCGC TTCAGCGTCG GGGGCATGAC TGACGTGGCT 
GAGATCAAGG GCCACAGGCG GACCTACGTG GGCGCCATGC CCGGGAAGAT CATCCAGTGT 
TTGAAGAAGA CCAAGACGGA GAACCCCCTG ATCCTCATCG ACGAGGTGGA CAAGATCGGC 
CGAGGCTACC AGGGGGACCC GTCGTCGGCA CTGCTGGAGC TGCTGGACCC AGAGCAGAAT 
GCCAACTTCC TGGACCACTA CCTGGACGTG CCCGTGGACT TGTCCAAGGT GCTGTTCATC 
TGCACGGCCA ACGTCACGGA CACCATCCCC GAGCCGCTGC GAGACCGTAT GGAGATGATC 
AACGTGTCGG GCTACGTGGC CCAGGAGAAG CTGGCCATTG CGGAGCGCTA CCTGGTGCCC 
CAGGCTCGCG CCCTGTGTGG CTTGGATGAG AGCAAGGCCA AGCTGTCATC GGACGTGCTG 
ACGCTGCTCA TCAAGCAGTA CTGCCGCGAG AGCGGTGTCC GCAACCTGCA GAAGCAAGTG 
GAGAAGGTGT TACGGAAATC GGCCTACAAG ATTGTCAGCG GCGAGGCCGA GTCCGTGGAG 
GTGACGCCCG AGAACCTGCA GGACTTCGTG GGGAAGCCCG TGTTCACCGT GGAGCGCATG 
TATGACGTGA CACCGCCCGG CGTGGTCATG GGGCTGGCCT GGACCGCAAT GGGAGGCTCC 
ACGCTGTTTG TGGAGACATC CCTGAGACGG CCACAGGACA AGGATGCCAA GGGTGACAAG 
GATGGCAGCC TGGAGGTGAC AGGCCAGCTG GGGGAGGTGA TGAAGGAGAG CGCCCGCATA 
GCCTACACCT TCGCCAGAGC CTTCCTCATG CAGCACGCCC CCGCCAATGA CTACCTGGTG 
ACCTCACACA TCCACCTGCA TGTGCCCGAG GGCGCCACCC CCAAGGACGG CCCAAGCGCA 
GGCTGCACCA TCGTCACGGC CCTGCTGTCC CTGGCCATGG GCAGGCCTGT CCGGCAGAAT 
CTGGCCATGA CTGGCGAAGT CTCCCTCACG GGCAAGATCC TGCCTGTTGG TGGCATCAAG 
GAGAAGACCA TTGCGGCCAA GCGCGCAGGG GTGACGTGCA TCGTCCTGCC AGCCGAGAAC 
AAGAAGGACT TCTACGACCT GGCAGCCTTC ATCACCGAGG GCCTGGAGGT GCACTTCGTG 
GAACACTACC GGGAGATCTT CGACATCGCC TTCCCGGACG AGCAGGCAGA GGCGCTGGCC 
GTGGAACGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LONP1 ( NM_004793.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004784.2 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20290
Clone ID Related Accession (Same CDS sequence)	NM_004793.4 , NM_004793.3
Accession Version	NM_004793.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI906625.1 and BC000235.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004793.3. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000235.1, SRR1660803.30729.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000360614.8/ ENSP00000353826.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATGT CACCCGCAAC 
TACCTAGACT GGCTCACGTC CATCCCTTGG GGCAAGTACA GCAACGAGAA CCTGGACCTG 
GCGCGGGCAC AGGCAGTGCT GGAGGAAGAC CACTACGGCA TGGAGGACGT CAAGAAACGC 
ATCCTGGAGT TCATTGCCGT TAGCCAGCTC CGCGGCTCCA CCCAGGGCAA GATCCTCTGC 
TTCTATGGCC CCCCTGGCGT GGGTAAGACC AGCATTGCTC GCTCCATCGC CCGCGCCCTG 
AACCGAGAGT ACTTCCGCTT CAGCGTCGGG GGCATGACTG ACGTGGCTGA GATCAAGGGC 
CACAGGCGGA CCTACGTGGG CGCCATGCCC GGGAAGATCA TCCAGTGTTT GAAGAAGACC 
AAGACGGAGA ACCCCCTGAT CCTCATCGAC GAGGTGGACA AGATCGGCCG AGGCTACCAG 
GGGGACCCGT CGTCGGCACT GCTGGAGCTG CTGGACCCAG AGCAGAATGC CAACTTCCTG 
GACCACTACC TGGACGTGCC CGTGGACTTG TCCAAGGTGC TGTTCATCTG CACGGCCAAC 
GTCACGGACA CCATCCCCGA GCCGCTGCGA GACCGTATGG AGATGATCAA CGTGTCGGGC 
TACGTGGCCC AGGAGAAGCT GGCCATTGCG GAGCGCTACC TGGTGCCCCA GGCTCGCGCC 
CTGTGTGGCT TGGATGAGAG CAAGGCCAAG CTGTCATCGG ACGTGCTGAC GCTGCTCATC 
AAGCAGTACT GCCGCGAGAG CGGTGTCCGC AACCTGCAGA AGCAAGTGGA GAAGGTGTTA 
CGGAAATCGG CCTACAAGAT TGTCAGCGGC GAGGCCGAGT CCGTGGAGGT GACGCCCGAG 
AACCTGCAGG ACTTCGTGGG GAAGCCCGTG TTCACCGTGG AGCGCATGTA TGACGTGACA 
CCGCCCGGCG TGGTCATGGG GCTGGCCTGG ACCGCAATGG GAGGCTCCAC GCTGTTTGTG 
GAGACATCCC TGAGACGGCC ACAGGACAAG GATGCCAAGG GTGACAAGGA TGGCAGCCTG 
GAGGTGACAG GCCAGCTGGG GGAGGTGATG AAGGAGAGCG CCCGCATAGC CTACACCTTC 
GCCAGAGCCT TCCTCATGCA GCACGCCCCC GCCAATGACT ACCTGGTGAC CTCACACATC 
CACCTGCATG TGCCCGAGGG CGCCACCCCC AAGGACGGCC CAAGCGCAGG CTGCACCATC 
GTCACGGCCC TGCTGTCCCT GGCCATGGGC AGGCCTGTCC GGCAGAATCT GGCCATGACT 
GGCGAAGTCT CCCTCACGGG CAAGATCCTG CCTGTTGGTG GCATCAAGGA GAAGACCATT 
GCGGCCAAGC GCGCAGGGGT GACGTGCATC GTCCTGCCAG CCGAGAACAA GAAGGACTTC 
TACGACCTGG CAGCCTTCAT CACCGAGGGC CTGGAGGTGC ACTTCGTGGA ACACTACCGG 
GAGATCTTCG ACATCGCCTT CCCGGACGAG CAGGCAGAGG CGCTGGCCGT GGAACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004784.2
CDS	15..2894
Translation	MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGQRTCDASPPWALWGRGPAIGGQWRGFWEASSRGGGAFSGGEDASEGGAEEGAGGAGGSAGAGEGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

Target ORF information:

RefSeq Version	NM_004793.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004793.4

ORF Insert Sequence:

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATGT CACCCGCAAC 
TACCTAGACT GGCTCACGTC CATCCCTTGG GGCAAGTACA GCAACGAGAA CCTGGACCTG 
GCGCGGGCAC AGGCAGTGCT GGAGGAAGAC CACTACGGCA TGGAGGACGT CAAGAAACGC 
ATCCTGGAGT TCATTGCCGT TAGCCAGCTC CGCGGCTCCA CCCAGGGCAA GATCCTCTGC 
TTCTATGGCC CCCCTGGCGT GGGTAAGACC AGCATTGCTC GCTCCATCGC CCGCGCCCTG 
AACCGAGAGT ACTTCCGCTT CAGCGTCGGG GGCATGACTG ACGTGGCTGA GATCAAGGGC 
CACAGGCGGA CCTACGTGGG CGCCATGCCC GGGAAGATCA TCCAGTGTTT GAAGAAGACC 
AAGACGGAGA ACCCCCTGAT CCTCATCGAC GAGGTGGACA AGATCGGCCG AGGCTACCAG 
GGGGACCCGT CGTCGGCACT GCTGGAGCTG CTGGACCCAG AGCAGAATGC CAACTTCCTG 
GACCACTACC TGGACGTGCC CGTGGACTTG TCCAAGGTGC TGTTCATCTG CACGGCCAAC 
GTCACGGACA CCATCCCCGA GCCGCTGCGA GACCGTATGG AGATGATCAA CGTGTCGGGC 
TACGTGGCCC AGGAGAAGCT GGCCATTGCG GAGCGCTACC TGGTGCCCCA GGCTCGCGCC 
CTGTGTGGCT TGGATGAGAG CAAGGCCAAG CTGTCATCGG ACGTGCTGAC GCTGCTCATC 
AAGCAGTACT GCCGCGAGAG CGGTGTCCGC AACCTGCAGA AGCAAGTGGA GAAGGTGTTA 
CGGAAATCGG CCTACAAGAT TGTCAGCGGC GAGGCCGAGT CCGTGGAGGT GACGCCCGAG 
AACCTGCAGG ACTTCGTGGG GAAGCCCGTG TTCACCGTGG AGCGCATGTA TGACGTGACA 
CCGCCCGGCG TGGTCATGGG GCTGGCCTGG ACCGCAATGG GAGGCTCCAC GCTGTTTGTG 
GAGACATCCC TGAGACGGCC ACAGGACAAG GATGCCAAGG GTGACAAGGA TGGCAGCCTG 
GAGGTGACAG GCCAGCTGGG GGAGGTGATG AAGGAGAGCG CCCGCATAGC CTACACCTTC 
GCCAGAGCCT TCCTCATGCA GCACGCCCCC GCCAATGACT ACCTGGTGAC CTCACACATC 
CACCTGCATG TGCCCGAGGG CGCCACCCCC AAGGACGGCC CAAGCGCAGG CTGCACCATC 
GTCACGGCCC TGCTGTCCCT GGCCATGGGC AGGCCTGTCC GGCAGAATCT GGCCATGACT 
GGCGAAGTCT CCCTCACGGG CAAGATCCTG CCTGTTGGTG GCATCAAGGA GAAGACCATT 
GCGGCCAAGC GCGCAGGGGT GACGTGCATC GTCCTGCCAG CCGAGAACAA GAAGGACTTC 
TACGACCTGG CAGCCTTCAT CACCGAGGGC CTGGAGGTGC ACTTCGTGGA ACACTACCGG 
GAGATCTTCG ACATCGCCTT CCCGGACGAG CAGGCAGAGG CGCTGGCCGT GGAACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LONP1 ( NM_004793.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004784.2 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 1, mRNA.

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CloneID	OHu20290
Clone ID Related Accession (Same CDS sequence)	NM_004793.4 , NM_004793.3
Accession Version	NM_004793.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2880bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI906625.1 and BC000235.1. This sequence is a reference standard in the RefSeqGene project. On Feb 15, 2013 this sequence version replaced NM_004793.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U02389.1, SRR1803617.156155.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATGT CACCCGCAAC 
TACCTAGACT GGCTCACGTC CATCCCTTGG GGCAAGTACA GCAACGAGAA CCTGGACCTG 
GCGCGGGCAC AGGCAGTGCT GGAGGAAGAC CACTACGGCA TGGAGGACGT CAAGAAACGC 
ATCCTGGAGT TCATTGCCGT TAGCCAGCTC CGCGGCTCCA CCCAGGGCAA GATCCTCTGC 
TTCTATGGCC CCCCTGGCGT GGGTAAGACC AGCATTGCTC GCTCCATCGC CCGCGCCCTG 
AACCGAGAGT ACTTCCGCTT CAGCGTCGGG GGCATGACTG ACGTGGCTGA GATCAAGGGC 
CACAGGCGGA CCTACGTGGG CGCCATGCCC GGGAAGATCA TCCAGTGTTT GAAGAAGACC 
AAGACGGAGA ACCCCCTGAT CCTCATCGAC GAGGTGGACA AGATCGGCCG AGGCTACCAG 
GGGGACCCGT CGTCGGCACT GCTGGAGCTG CTGGACCCAG AGCAGAATGC CAACTTCCTG 
GACCACTACC TGGACGTGCC CGTGGACTTG TCCAAGGTGC TGTTCATCTG CACGGCCAAC 
GTCACGGACA CCATCCCCGA GCCGCTGCGA GACCGTATGG AGATGATCAA CGTGTCGGGC 
TACGTGGCCC AGGAGAAGCT GGCCATTGCG GAGCGCTACC TGGTGCCCCA GGCTCGCGCC 
CTGTGTGGCT TGGATGAGAG CAAGGCCAAG CTGTCATCGG ACGTGCTGAC GCTGCTCATC 
AAGCAGTACT GCCGCGAGAG CGGTGTCCGC AACCTGCAGA AGCAAGTGGA GAAGGTGTTA 
CGGAAATCGG CCTACAAGAT TGTCAGCGGC GAGGCCGAGT CCGTGGAGGT GACGCCCGAG 
AACCTGCAGG ACTTCGTGGG GAAGCCCGTG TTCACCGTGG AGCGCATGTA TGACGTGACA 
CCGCCCGGCG TGGTCATGGG GCTGGCCTGG ACCGCAATGG GAGGCTCCAC GCTGTTTGTG 
GAGACATCCC TGAGACGGCC ACAGGACAAG GATGCCAAGG GTGACAAGGA TGGCAGCCTG 
GAGGTGACAG GCCAGCTGGG GGAGGTGATG AAGGAGAGCG CCCGCATAGC CTACACCTTC 
GCCAGAGCCT TCCTCATGCA GCACGCCCCC GCCAATGACT ACCTGGTGAC CTCACACATC 
CACCTGCATG TGCCCGAGGG CGCCACCCCC AAGGACGGCC CAAGCGCAGG CTGCACCATC 
GTCACGGCCC TGCTGTCCCT GGCCATGGGC AGGCCTGTCC GGCAGAATCT GGCCATGACT 
GGCGAAGTCT CCCTCACGGG CAAGATCCTG CCTGTTGGTG GCATCAAGGA GAAGACCATT 
GCGGCCAAGC GCGCAGGGGT GACGTGCATC GTCCTGCCAG CCGAGAACAA GAAGGACTTC 
TACGACCTGG CAGCCTTCAT CACCGAGGGC CTGGAGGTGC ACTTCGTGGA ACACTACCGG 
GAGATCTTCG ACATCGCCTT CCCGGACGAG CAGGCAGAGG CGCTGGCCGT GGAACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004784.2
CDS	127..3006
Translation	MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGQRTCDASPPWALWGRGPAIGGQWRGFWEASSRGGGAFSGGEDASEGGAEEGAGGAGGSAGAGEGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

Target ORF information:

RefSeq Version	NM_004793.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004793.3

ORF Insert Sequence:

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATGT CACCCGCAAC 
TACCTAGACT GGCTCACGTC CATCCCTTGG GGCAAGTACA GCAACGAGAA CCTGGACCTG 
GCGCGGGCAC AGGCAGTGCT GGAGGAAGAC CACTACGGCA TGGAGGACGT CAAGAAACGC 
ATCCTGGAGT TCATTGCCGT TAGCCAGCTC CGCGGCTCCA CCCAGGGCAA GATCCTCTGC 
TTCTATGGCC CCCCTGGCGT GGGTAAGACC AGCATTGCTC GCTCCATCGC CCGCGCCCTG 
AACCGAGAGT ACTTCCGCTT CAGCGTCGGG GGCATGACTG ACGTGGCTGA GATCAAGGGC 
CACAGGCGGA CCTACGTGGG CGCCATGCCC GGGAAGATCA TCCAGTGTTT GAAGAAGACC 
AAGACGGAGA ACCCCCTGAT CCTCATCGAC GAGGTGGACA AGATCGGCCG AGGCTACCAG 
GGGGACCCGT CGTCGGCACT GCTGGAGCTG CTGGACCCAG AGCAGAATGC CAACTTCCTG 
GACCACTACC TGGACGTGCC CGTGGACTTG TCCAAGGTGC TGTTCATCTG CACGGCCAAC 
GTCACGGACA CCATCCCCGA GCCGCTGCGA GACCGTATGG AGATGATCAA CGTGTCGGGC 
TACGTGGCCC AGGAGAAGCT GGCCATTGCG GAGCGCTACC TGGTGCCCCA GGCTCGCGCC 
CTGTGTGGCT TGGATGAGAG CAAGGCCAAG CTGTCATCGG ACGTGCTGAC GCTGCTCATC 
AAGCAGTACT GCCGCGAGAG CGGTGTCCGC AACCTGCAGA AGCAAGTGGA GAAGGTGTTA 
CGGAAATCGG CCTACAAGAT TGTCAGCGGC GAGGCCGAGT CCGTGGAGGT GACGCCCGAG 
AACCTGCAGG ACTTCGTGGG GAAGCCCGTG TTCACCGTGG AGCGCATGTA TGACGTGACA 
CCGCCCGGCG TGGTCATGGG GCTGGCCTGG ACCGCAATGG GAGGCTCCAC GCTGTTTGTG 
GAGACATCCC TGAGACGGCC ACAGGACAAG GATGCCAAGG GTGACAAGGA TGGCAGCCTG 
GAGGTGACAG GCCAGCTGGG GGAGGTGATG AAGGAGAGCG CCCGCATAGC CTACACCTTC 
GCCAGAGCCT TCCTCATGCA GCACGCCCCC GCCAATGACT ACCTGGTGAC CTCACACATC 
CACCTGCATG TGCCCGAGGG CGCCACCCCC AAGGACGGCC CAAGCGCAGG CTGCACCATC 
GTCACGGCCC TGCTGTCCCT GGCCATGGGC AGGCCTGTCC GGCAGAATCT GGCCATGACT 
GGCGAAGTCT CCCTCACGGG CAAGATCCTG CCTGTTGGTG GCATCAAGGA GAAGACCATT 
GCGGCCAAGC GCGCAGGGGT GACGTGCATC GTCCTGCCAG CCGAGAACAA GAAGGACTTC 
TACGACCTGG CAGCCTTCAT CACCGAGGGC CTGGAGGTGC ACTTCGTGGA ACACTACCGG 
GAGATCTTCG ACATCGCCTT CCCGGACGAG CAGGCAGAGG CGCTGGCCGT GGAACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LONP1 ( NM_001276479.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263408.1 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2, mRNA.

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CloneID	OHu10182
Clone ID Related Accession (Same CDS sequence)	NM_001276479.1 , NM_001276479.2
Accession Version	NM_001276479.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096626.1 and BC000235.1. Transcript Variant: This variant (2) is alternatively spliced at the 5' end compared to variant 1. It uses the same translation start codon as variant 1, however, the encoded isoform (2) lacks a 64 aa protein segment in the 5' coding region compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096626.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCCGG TCATAACGGC GCTCACGCCC ATGACGATCC CCGATGTGTT TCCGCACCTG 
CCGCTCATCG CCATCACCCG CAACCCGGTG TTCCCGCGCT TTATCAAGAT TATCGAGGTT 
AAAAATAAGA AGTTGGTTGA GCTGCTGAGA AGGAAAGTTC GTCTCGCCCA GCCTTATGTC 
GGCGTCTTTC TAAAGAGAGA TGACAGCAAT GAGTCGGATG TGGTCGAGAG CCTGGATGAA 
ATCTACCACA CGGGGACGTT TGCCCAGATC CATGAGATGC AGGACCTTGG GGACAAGCTG 
CGCATGATCG TCATGGGACA CAGAAGAGTC CATATCAGCA GACAGCTGGA GGTGGAGCCC 
GAGGAGCCGG AGGCGGAGAA CAAGCACAAG CCCCGCAGGA AGTCAAAGCG GGGCAAGAAG 
GAGGCGGAGG ACGAGCTGAG CGCCAGGCAC CCGGCGGAGC TGGCGATGGA GCCCACCCCT 
GAGCTCCCGG CTGAGGTGCT CATGGTGGAG GTAGAGAACG TTGTCCACGA GGACTTCCAG 
GTCACGGAGG AGGTGAAAGC CCTGACTGCA GAGATCGTGA AGACCATCCG GGACATCATT 
GCCTTGAACC CTCTCTACAG GGAGTCAGTG CTGCAGATGA TGCAGGCTGG CCAGCGGGTG 
GTGGACAACC CCATCTACCT GAGCGACATG GGCGCCGCGC TCACCGGGGC CGAGTCCCAT 
GAGCTGCAGG ACGTCCTGGA AGAGACCAAT ATTCCTAAGC GGCTGTACAA GGCCCTCTCC 
CTGCTGAAGA AGGAATTTGA ACTGAGCAAG CTGCAGCAGC GCCTGGGGCG GGAGGTGGAG 
GAGAAGATCA AGCAGACCCA CCGTAAGTAC CTGCTGCAGG AGCAGCTAAA GATCATCAAG 
AAGGAGCTGG GCCTGGAGAA GGACGACAAG GATGCCATCG AGGAGAAGTT CCGGGAGCGC 
CTGAAGGAGC TCGTGGTCCC CAAGCACGTC ATGGATGTTG TGGACGAGGA GCTGAGCAAG 
CTGGGCCTGC TGGACAACCA CTCCTCGGAG TTCAATGTCA CCCGCAACTA CCTAGACTGG 
CTCACGTCCA TCCCTTGGGG CAAGTACAGC AACGAGAACC TGGACCTGGC GCGGGCACAG 
GCAGTGCTGG AGGAAGACCA CTACGGCATG GAGGACGTCA AGAAACGCAT CCTGGAGTTC 
ATTGCCGTTA GCCAGCTCCG CGGCTCCACC CAGGGCAAGA TCCTCTGCTT CTATGGCCCC 
CCTGGCGTGG GTAAGACCAG CATTGCTCGC TCCATCGCCC GCGCCCTGAA CCGAGAGTAC 
TTCCGCTTCA GCGTCGGGGG CATGACTGAC GTGGCTGAGA TCAAGGGCCA CAGGCGGACC 
TACGTGGGCG CCATGCCCGG GAAGATCATC CAGTGTTTGA AGAAGACCAA GACGGAGAAC 
CCCCTGATCC TCATCGACGA GGTGGACAAG ATCGGCCGAG GCTACCAGGG GGACCCGTCG 
TCGGCACTGC TGGAGCTGCT GGACCCAGAG CAGAATGCCA ACTTCCTGGA CCACTACCTG 
GACGTGCCCG TGGACTTGTC CAAGGTGCTG TTCATCTGCA CGGCCAACGT CACGGACACC 
ATCCCCGAGC CGCTGCGAGA CCGTATGGAG ATGATCAACG TGTCGGGCTA CGTGGCCCAG 
GAGAAGCTGG CCATTGCGGA GCGCTACCTG GTGCCCCAGG CTCGCGCCCT GTGTGGCTTG 
GATGAGAGCA AGGCCAAGCT GTCATCGGAC GTGCTGACGC TGCTCATCAA GCAGTACTGC 
CGCGAGAGCG GTGTCCGCAA CCTGCAGAAG CAAGTGGAGA AGGTGTTACG GAAATCGGCC 
TACAAGATTG TCAGCGGCGA GGCCGAGTCC GTGGAGGTGA CGCCCGAGAA CCTGCAGGAC 
TTCGTGGGGA AGCCCGTGTT CACCGTGGAG CGCATGTATG ACGTGACACC GCCCGGCGTG 
GTCATGGGGC TGGCCTGGAC CGCAATGGGA GGCTCCACGC TGTTTGTGGA GACATCCCTG 
AGACGGCCAC AGGACAAGGA TGCCAAGGGT GACAAGGATG GCAGCCTGGA GGTGACAGGC 
CAGCTGGGGG AGGTGATGAA GGAGAGCGCC CGCATAGCCT ACACCTTCGC CAGAGCCTTC 
CTCATGCAGC ACGCCCCCGC CAATGACTAC CTGGTGACCT CACACATCCA CCTGCATGTG 
CCCGAGGGCG CCACCCCCAA GGACGGCCCA AGCGCAGGCT GCACCATCGT CACGGCCCTG 
CTGTCCCTGG CCATGGGCAG GCCTGTCCGG CAGAATCTGG CCATGACTGG CGAAGTCTCC 
CTCACGGGCA AGATCCTGCC TGTTGGTGGC ATCAAGGAGA AGACCATTGC GGCCAAGCGC 
GCAGGGGTGA CGTGCATCGT CCTGCCAGCC GAGAACAAGA AGGACTTCTA CGACCTGGCA 
GCCTTCATCA CCGAGGGCCT GGAGGTGCAC TTCGTGGAAC ACTACCGGGA GATCTTCGAC 
ATCGCCTTCC CGGACGAGCA GGCAGAGGCG CTGGCCGTGG AACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263408.1
CDS	34..2721
Translation	MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

Target ORF information:

RefSeq Version	NM_001276479.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276479.1

ORF Insert Sequence:

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCCGG TCATAACGGC GCTCACGCCC ATGACGATCC CCGATGTGTT TCCGCACCTG 
CCGCTCATCG CCATCACCCG CAACCCGGTG TTCCCGCGCT TTATCAAGAT TATCGAGGTT 
AAAAATAAGA AGTTGGTTGA GCTGCTGAGA AGGAAAGTTC GTCTCGCCCA GCCTTATGTC 
GGCGTCTTTC TAAAGAGAGA TGACAGCAAT GAGTCGGATG TGGTCGAGAG CCTGGATGAA 
ATCTACCACA CGGGGACGTT TGCCCAGATC CATGAGATGC AGGACCTTGG GGACAAGCTG 
CGCATGATCG TCATGGGACA CAGAAGAGTC CATATCAGCA GACAGCTGGA GGTGGAGCCC 
GAGGAGCCGG AGGCGGAGAA CAAGCACAAG CCCCGCAGGA AGTCAAAGCG GGGCAAGAAG 
GAGGCGGAGG ACGAGCTGAG CGCCAGGCAC CCGGCGGAGC TGGCGATGGA GCCCACCCCT 
GAGCTCCCGG CTGAGGTGCT CATGGTGGAG GTAGAGAACG TTGTCCACGA GGACTTCCAG 
GTCACGGAGG AGGTGAAAGC CCTGACTGCA GAGATCGTGA AGACCATCCG GGACATCATT 
GCCTTGAACC CTCTCTACAG GGAGTCAGTG CTGCAGATGA TGCAGGCTGG CCAGCGGGTG 
GTGGACAACC CCATCTACCT GAGCGACATG GGCGCCGCGC TCACCGGGGC CGAGTCCCAT 
GAGCTGCAGG ACGTCCTGGA AGAGACCAAT ATTCCTAAGC GGCTGTACAA GGCCCTCTCC 
CTGCTGAAGA AGGAATTTGA ACTGAGCAAG CTGCAGCAGC GCCTGGGGCG GGAGGTGGAG 
GAGAAGATCA AGCAGACCCA CCGTAAGTAC CTGCTGCAGG AGCAGCTAAA GATCATCAAG 
AAGGAGCTGG GCCTGGAGAA GGACGACAAG GATGCCATCG AGGAGAAGTT CCGGGAGCGC 
CTGAAGGAGC TCGTGGTCCC CAAGCACGTC ATGGATGTTG TGGACGAGGA GCTGAGCAAG 
CTGGGCCTGC TGGACAACCA CTCCTCGGAG TTCAATGTCA CCCGCAACTA CCTAGACTGG 
CTCACGTCCA TCCCTTGGGG CAAGTACAGC AACGAGAACC TGGACCTGGC GCGGGCACAG 
GCAGTGCTGG AGGAAGACCA CTACGGCATG GAGGACGTCA AGAAACGCAT CCTGGAGTTC 
ATTGCCGTTA GCCAGCTCCG CGGCTCCACC CAGGGCAAGA TCCTCTGCTT CTATGGCCCC 
CCTGGCGTGG GTAAGACCAG CATTGCTCGC TCCATCGCCC GCGCCCTGAA CCGAGAGTAC 
TTCCGCTTCA GCGTCGGGGG CATGACTGAC GTGGCTGAGA TCAAGGGCCA CAGGCGGACC 
TACGTGGGCG CCATGCCCGG GAAGATCATC CAGTGTTTGA AGAAGACCAA GACGGAGAAC 
CCCCTGATCC TCATCGACGA GGTGGACAAG ATCGGCCGAG GCTACCAGGG GGACCCGTCG 
TCGGCACTGC TGGAGCTGCT GGACCCAGAG CAGAATGCCA ACTTCCTGGA CCACTACCTG 
GACGTGCCCG TGGACTTGTC CAAGGTGCTG TTCATCTGCA CGGCCAACGT CACGGACACC 
ATCCCCGAGC CGCTGCGAGA CCGTATGGAG ATGATCAACG TGTCGGGCTA CGTGGCCCAG 
GAGAAGCTGG CCATTGCGGA GCGCTACCTG GTGCCCCAGG CTCGCGCCCT GTGTGGCTTG 
GATGAGAGCA AGGCCAAGCT GTCATCGGAC GTGCTGACGC TGCTCATCAA GCAGTACTGC 
CGCGAGAGCG GTGTCCGCAA CCTGCAGAAG CAAGTGGAGA AGGTGTTACG GAAATCGGCC 
TACAAGATTG TCAGCGGCGA GGCCGAGTCC GTGGAGGTGA CGCCCGAGAA CCTGCAGGAC 
TTCGTGGGGA AGCCCGTGTT CACCGTGGAG CGCATGTATG ACGTGACACC GCCCGGCGTG 
GTCATGGGGC TGGCCTGGAC CGCAATGGGA GGCTCCACGC TGTTTGTGGA GACATCCCTG 
AGACGGCCAC AGGACAAGGA TGCCAAGGGT GACAAGGATG GCAGCCTGGA GGTGACAGGC 
CAGCTGGGGG AGGTGATGAA GGAGAGCGCC CGCATAGCCT ACACCTTCGC CAGAGCCTTC 
CTCATGCAGC ACGCCCCCGC CAATGACTAC CTGGTGACCT CACACATCCA CCTGCATGTG 
CCCGAGGGCG CCACCCCCAA GGACGGCCCA AGCGCAGGCT GCACCATCGT CACGGCCCTG 
CTGTCCCTGG CCATGGGCAG GCCTGTCCGG CAGAATCTGG CCATGACTGG CGAAGTCTCC 
CTCACGGGCA AGATCCTGCC TGTTGGTGGC ATCAAGGAGA AGACCATTGC GGCCAAGCGC 
GCAGGGGTGA CGTGCATCGT CCTGCCAGCC GAGAACAAGA AGGACTTCTA CGACCTGGCA 
GCCTTCATCA CCGAGGGCCT GGAGGTGCAC TTCGTGGAAC ACTACCGGGA GATCTTCGAC 
ATCGCCTTCC CGGACGAGCA GGCAGAGGCG CTGGCCGTGG AACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LONP1 ( NM_001276479.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001263408.1 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2, mRNA.

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CloneID	OHu10182
Clone ID Related Accession (Same CDS sequence)	NM_001276479.1 , NM_001276479.2
Accession Version	NM_001276479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2688bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096626.1 and BC000235.1. On May 31, 2019 this sequence version replaced NM_001276479.1. Transcript Variant: This variant (2) is alternatively spliced at the 5' end compared to variant 1. It uses the same translation start codon as variant 1, however, the encoded isoform (2) lacks a 64 aa protein segment in the 5' coding region compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCCGG TCATAACGGC GCTCACGCCC ATGACGATCC CCGATGTGTT TCCGCACCTG 
CCGCTCATCG CCATCACCCG CAACCCGGTG TTCCCGCGCT TTATCAAGAT TATCGAGGTT 
AAAAATAAGA AGTTGGTTGA GCTGCTGAGA AGGAAAGTTC GTCTCGCCCA GCCTTATGTC 
GGCGTCTTTC TAAAGAGAGA TGACAGCAAT GAGTCGGATG TGGTCGAGAG CCTGGATGAA 
ATCTACCACA CGGGGACGTT TGCCCAGATC CATGAGATGC AGGACCTTGG GGACAAGCTG 
CGCATGATCG TCATGGGACA CAGAAGAGTC CATATCAGCA GACAGCTGGA GGTGGAGCCC 
GAGGAGCCGG AGGCGGAGAA CAAGCACAAG CCCCGCAGGA AGTCAAAGCG GGGCAAGAAG 
GAGGCGGAGG ACGAGCTGAG CGCCAGGCAC CCGGCGGAGC TGGCGATGGA GCCCACCCCT 
GAGCTCCCGG CTGAGGTGCT CATGGTGGAG GTAGAGAACG TTGTCCACGA GGACTTCCAG 
GTCACGGAGG AGGTGAAAGC CCTGACTGCA GAGATCGTGA AGACCATCCG GGACATCATT 
GCCTTGAACC CTCTCTACAG GGAGTCAGTG CTGCAGATGA TGCAGGCTGG CCAGCGGGTG 
GTGGACAACC CCATCTACCT GAGCGACATG GGCGCCGCGC TCACCGGGGC CGAGTCCCAT 
GAGCTGCAGG ACGTCCTGGA AGAGACCAAT ATTCCTAAGC GGCTGTACAA GGCCCTCTCC 
CTGCTGAAGA AGGAATTTGA ACTGAGCAAG CTGCAGCAGC GCCTGGGGCG GGAGGTGGAG 
GAGAAGATCA AGCAGACCCA CCGTAAGTAC CTGCTGCAGG AGCAGCTAAA GATCATCAAG 
AAGGAGCTGG GCCTGGAGAA GGACGACAAG GATGCCATCG AGGAGAAGTT CCGGGAGCGC 
CTGAAGGAGC TCGTGGTCCC CAAGCACGTC ATGGATGTTG TGGACGAGGA GCTGAGCAAG 
CTGGGCCTGC TGGACAACCA CTCCTCGGAG TTCAATGTCA CCCGCAACTA CCTAGACTGG 
CTCACGTCCA TCCCTTGGGG CAAGTACAGC AACGAGAACC TGGACCTGGC GCGGGCACAG 
GCAGTGCTGG AGGAAGACCA CTACGGCATG GAGGACGTCA AGAAACGCAT CCTGGAGTTC 
ATTGCCGTTA GCCAGCTCCG CGGCTCCACC CAGGGCAAGA TCCTCTGCTT CTATGGCCCC 
CCTGGCGTGG GTAAGACCAG CATTGCTCGC TCCATCGCCC GCGCCCTGAA CCGAGAGTAC 
TTCCGCTTCA GCGTCGGGGG CATGACTGAC GTGGCTGAGA TCAAGGGCCA CAGGCGGACC 
TACGTGGGCG CCATGCCCGG GAAGATCATC CAGTGTTTGA AGAAGACCAA GACGGAGAAC 
CCCCTGATCC TCATCGACGA GGTGGACAAG ATCGGCCGAG GCTACCAGGG GGACCCGTCG 
TCGGCACTGC TGGAGCTGCT GGACCCAGAG CAGAATGCCA ACTTCCTGGA CCACTACCTG 
GACGTGCCCG TGGACTTGTC CAAGGTGCTG TTCATCTGCA CGGCCAACGT CACGGACACC 
ATCCCCGAGC CGCTGCGAGA CCGTATGGAG ATGATCAACG TGTCGGGCTA CGTGGCCCAG 
GAGAAGCTGG CCATTGCGGA GCGCTACCTG GTGCCCCAGG CTCGCGCCCT GTGTGGCTTG 
GATGAGAGCA AGGCCAAGCT GTCATCGGAC GTGCTGACGC TGCTCATCAA GCAGTACTGC 
CGCGAGAGCG GTGTCCGCAA CCTGCAGAAG CAAGTGGAGA AGGTGTTACG GAAATCGGCC 
TACAAGATTG TCAGCGGCGA GGCCGAGTCC GTGGAGGTGA CGCCCGAGAA CCTGCAGGAC 
TTCGTGGGGA AGCCCGTGTT CACCGTGGAG CGCATGTATG ACGTGACACC GCCCGGCGTG 
GTCATGGGGC TGGCCTGGAC CGCAATGGGA GGCTCCACGC TGTTTGTGGA GACATCCCTG 
AGACGGCCAC AGGACAAGGA TGCCAAGGGT GACAAGGATG GCAGCCTGGA GGTGACAGGC 
CAGCTGGGGG AGGTGATGAA GGAGAGCGCC CGCATAGCCT ACACCTTCGC CAGAGCCTTC 
CTCATGCAGC ACGCCCCCGC CAATGACTAC CTGGTGACCT CACACATCCA CCTGCATGTG 
CCCGAGGGCG CCACCCCCAA GGACGGCCCA AGCGCAGGCT GCACCATCGT CACGGCCCTG 
CTGTCCCTGG CCATGGGCAG GCCTGTCCGG CAGAATCTGG CCATGACTGG CGAAGTCTCC 
CTCACGGGCA AGATCCTGCC TGTTGGTGGC ATCAAGGAGA AGACCATTGC GGCCAAGCGC 
GCAGGGGTGA CGTGCATCGT CCTGCCAGCC GAGAACAAGA AGGACTTCTA CGACCTGGCA 
GCCTTCATCA CCGAGGGCCT GGAGGTGCAC TTCGTGGAAC ACTACCGGGA GATCTTCGAC 
ATCGCCTTCC CGGACGAGCA GGCAGAGGCG CTGGCCGTGG AACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001263408.1
CDS	15..2702
Translation	MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER

Target ORF information:

RefSeq Version	NM_001276479.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001276479.2

ORF Insert Sequence:

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCCGG TCATAACGGC GCTCACGCCC ATGACGATCC CCGATGTGTT TCCGCACCTG 
CCGCTCATCG CCATCACCCG CAACCCGGTG TTCCCGCGCT TTATCAAGAT TATCGAGGTT 
AAAAATAAGA AGTTGGTTGA GCTGCTGAGA AGGAAAGTTC GTCTCGCCCA GCCTTATGTC 
GGCGTCTTTC TAAAGAGAGA TGACAGCAAT GAGTCGGATG TGGTCGAGAG CCTGGATGAA 
ATCTACCACA CGGGGACGTT TGCCCAGATC CATGAGATGC AGGACCTTGG GGACAAGCTG 
CGCATGATCG TCATGGGACA CAGAAGAGTC CATATCAGCA GACAGCTGGA GGTGGAGCCC 
GAGGAGCCGG AGGCGGAGAA CAAGCACAAG CCCCGCAGGA AGTCAAAGCG GGGCAAGAAG 
GAGGCGGAGG ACGAGCTGAG CGCCAGGCAC CCGGCGGAGC TGGCGATGGA GCCCACCCCT 
GAGCTCCCGG CTGAGGTGCT CATGGTGGAG GTAGAGAACG TTGTCCACGA GGACTTCCAG 
GTCACGGAGG AGGTGAAAGC CCTGACTGCA GAGATCGTGA AGACCATCCG GGACATCATT 
GCCTTGAACC CTCTCTACAG GGAGTCAGTG CTGCAGATGA TGCAGGCTGG CCAGCGGGTG 
GTGGACAACC CCATCTACCT GAGCGACATG GGCGCCGCGC TCACCGGGGC CGAGTCCCAT 
GAGCTGCAGG ACGTCCTGGA AGAGACCAAT ATTCCTAAGC GGCTGTACAA GGCCCTCTCC 
CTGCTGAAGA AGGAATTTGA ACTGAGCAAG CTGCAGCAGC GCCTGGGGCG GGAGGTGGAG 
GAGAAGATCA AGCAGACCCA CCGTAAGTAC CTGCTGCAGG AGCAGCTAAA GATCATCAAG 
AAGGAGCTGG GCCTGGAGAA GGACGACAAG GATGCCATCG AGGAGAAGTT CCGGGAGCGC 
CTGAAGGAGC TCGTGGTCCC CAAGCACGTC ATGGATGTTG TGGACGAGGA GCTGAGCAAG 
CTGGGCCTGC TGGACAACCA CTCCTCGGAG TTCAATGTCA CCCGCAACTA CCTAGACTGG 
CTCACGTCCA TCCCTTGGGG CAAGTACAGC AACGAGAACC TGGACCTGGC GCGGGCACAG 
GCAGTGCTGG AGGAAGACCA CTACGGCATG GAGGACGTCA AGAAACGCAT CCTGGAGTTC 
ATTGCCGTTA GCCAGCTCCG CGGCTCCACC CAGGGCAAGA TCCTCTGCTT CTATGGCCCC 
CCTGGCGTGG GTAAGACCAG CATTGCTCGC TCCATCGCCC GCGCCCTGAA CCGAGAGTAC 
TTCCGCTTCA GCGTCGGGGG CATGACTGAC GTGGCTGAGA TCAAGGGCCA CAGGCGGACC 
TACGTGGGCG CCATGCCCGG GAAGATCATC CAGTGTTTGA AGAAGACCAA GACGGAGAAC 
CCCCTGATCC TCATCGACGA GGTGGACAAG ATCGGCCGAG GCTACCAGGG GGACCCGTCG 
TCGGCACTGC TGGAGCTGCT GGACCCAGAG CAGAATGCCA ACTTCCTGGA CCACTACCTG 
GACGTGCCCG TGGACTTGTC CAAGGTGCTG TTCATCTGCA CGGCCAACGT CACGGACACC 
ATCCCCGAGC CGCTGCGAGA CCGTATGGAG ATGATCAACG TGTCGGGCTA CGTGGCCCAG 
GAGAAGCTGG CCATTGCGGA GCGCTACCTG GTGCCCCAGG CTCGCGCCCT GTGTGGCTTG 
GATGAGAGCA AGGCCAAGCT GTCATCGGAC GTGCTGACGC TGCTCATCAA GCAGTACTGC 
CGCGAGAGCG GTGTCCGCAA CCTGCAGAAG CAAGTGGAGA AGGTGTTACG GAAATCGGCC 
TACAAGATTG TCAGCGGCGA GGCCGAGTCC GTGGAGGTGA CGCCCGAGAA CCTGCAGGAC 
TTCGTGGGGA AGCCCGTGTT CACCGTGGAG CGCATGTATG ACGTGACACC GCCCGGCGTG 
GTCATGGGGC TGGCCTGGAC CGCAATGGGA GGCTCCACGC TGTTTGTGGA GACATCCCTG 
AGACGGCCAC AGGACAAGGA TGCCAAGGGT GACAAGGATG GCAGCCTGGA GGTGACAGGC 
CAGCTGGGGG AGGTGATGAA GGAGAGCGCC CGCATAGCCT ACACCTTCGC CAGAGCCTTC 
CTCATGCAGC ACGCCCCCGC CAATGACTAC CTGGTGACCT CACACATCCA CCTGCATGTG 
CCCGAGGGCG CCACCCCCAA GGACGGCCCA AGCGCAGGCT GCACCATCGT CACGGCCCTG 
CTGTCCCTGG CCATGGGCAG GCCTGTCCGG CAGAATCTGG CCATGACTGG CGAAGTCTCC 
CTCACGGGCA AGATCCTGCC TGTTGGTGGC ATCAAGGAGA AGACCATTGC GGCCAAGCGC 
GCAGGGGTGA CGTGCATCGT CCTGCCAGCC GAGAACAAGA AGGACTTCTA CGACCTGGCA 
GCCTTCATCA CCGAGGGCCT GGAGGTGCAC TTCGTGGAAC ACTACCGGGA GATCTTCGAC 
ATCGCCTTCC CGGACGAGCA GGCAGAGGCG CTGGCCGTGG AACGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LONP1 ( XM_011528441.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526743.1 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant X1, mRNA.

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CloneID	OHu62723
Clone ID Related Accession (Same CDS sequence)	XM_011528441.2 , XM_011528441.3
Accession Version	XM_011528441.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATAT CTGGAGGCGC 
CCGCATCCCT CGGCTCGGGG CCCCTTCCTC CTTCACAGCC ACAGCGCAGC CTCTTCCAGT 
CAGTCTCTCA CCTCCCACCG CCTGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526743.1
CDS	170..1639
Translation	MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGQRTCDASPPWALWGRGPAIGGQWRGFWEASSRGGGAFSGGEDASEGGAEEGAGGAGGSAGAGEGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNIWRRPHPSARGPFLLHSHSAASSSQSLTSHRLS

Target ORF information:

RefSeq Version	XM_011528441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528441.2

ORF Insert Sequence:

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATAT CTGGAGGCGC 
CCGCATCCCT CGGCTCGGGG CCCCTTCCTC CTTCACAGCC ACAGCGCAGC CTCTTCCAGT 
CAGTCTCTCA CCTCCCACCG CCTGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LONP1 ( XM_011528441.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526743.1 Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant X1, mRNA.

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CloneID	OHu62723
Clone ID Related Accession (Same CDS sequence)	XM_011528441.2 , XM_011528441.3
Accession Version	XM_011528441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lon protease homolog, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATAT CTGGAGGCGC 
CCGCATCCCT CGGCTCGGGG CCCCTTCCTC CTTCACAGCC ACAGCGCAGC CTCTTCCAGT 
CAGTCTCTCA CCTCCCACCG CCTGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526743.1
CDS	15..1484
Translation	MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGQRTCDASPPWALWGRGPAIGGQWRGFWEASSRGGGAFSGGEDASEGGAEEGAGGAGGSAGAGEGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNIWRRPHPSARGPFLLHSHSAASSSQSLTSHRLS

Target ORF information:

RefSeq Version	XM_011528441.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lon peptidase 1, mitochondrial (LONP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528441.3

ORF Insert Sequence:

ATGGCGGCGA GCACTGGCTA CGTGCGACTG TGGGGAGCGG CGCGGTGCTG GGTGCTGCGG 
CGGCCGATGC TGGCCGCCGC CGGGGGGCGG GTTCCCACTG CAGCAGGAGC GTGGTTGCTC 
CGAGGCCAGC GGACCTGCGA CGCCTCTCCT CCTTGGGCAC TGTGGGGCCG AGGCCCGGCA 
ATTGGGGGCC AATGGCGGGG GTTTTGGGAA GCGAGCAGCC GCGGCGGAGG CGCATTCTCG 
GGGGGCGAGG ACGCCTCCGA GGGCGGCGCG GAGGAAGGAG CCGGCGGCGC GGGGGGCAGC 
GCGGGCGCCG GGGAAGGCCC GGTCATAACG GCGCTCACGC CCATGACGAT CCCCGATGTG 
TTTCCGCACC TGCCGCTCAT CGCCATCACC CGCAACCCGG TGTTCCCGCG CTTTATCAAG 
ATTATCGAGG TTAAAAATAA GAAGTTGGTT GAGCTGCTGA GAAGGAAAGT TCGTCTCGCC 
CAGCCTTATG TCGGCGTCTT TCTAAAGAGA GATGACAGCA ATGAGTCGGA TGTGGTCGAG 
AGCCTGGATG AAATCTACCA CACGGGGACG TTTGCCCAGA TCCATGAGAT GCAGGACCTT 
GGGGACAAGC TGCGCATGAT CGTCATGGGA CACAGAAGAG TCCATATCAG CAGACAGCTG 
GAGGTGGAGC CCGAGGAGCC GGAGGCGGAG AACAAGCACA AGCCCCGCAG GAAGTCAAAG 
CGGGGCAAGA AGGAGGCGGA GGACGAGCTG AGCGCCAGGC ACCCGGCGGA GCTGGCGATG 
GAGCCCACCC CTGAGCTCCC GGCTGAGGTG CTCATGGTGG AGGTAGAGAA CGTTGTCCAC 
GAGGACTTCC AGGTCACGGA GGAGGTGAAA GCCCTGACTG CAGAGATCGT GAAGACCATC 
CGGGACATCA TTGCCTTGAA CCCTCTCTAC AGGGAGTCAG TGCTGCAGAT GATGCAGGCT 
GGCCAGCGGG TGGTGGACAA CCCCATCTAC CTGAGCGACA TGGGCGCCGC GCTCACCGGG 
GCCGAGTCCC ATGAGCTGCA GGACGTCCTG GAAGAGACCA ATATTCCTAA GCGGCTGTAC 
AAGGCCCTCT CCCTGCTGAA GAAGGAATTT GAACTGAGCA AGCTGCAGCA GCGCCTGGGG 
CGGGAGGTGG AGGAGAAGAT CAAGCAGACC CACCGTAAGT ACCTGCTGCA GGAGCAGCTA 
AAGATCATCA AGAAGGAGCT GGGCCTGGAG AAGGACGACA AGGATGCCAT CGAGGAGAAG 
TTCCGGGAGC GCCTGAAGGA GCTCGTGGTC CCCAAGCACG TCATGGATGT TGTGGACGAG 
GAGCTGAGCA AGCTGGGCCT GCTGGACAAC CACTCCTCGG AGTTCAATAT CTGGAGGCGC 
CCGCATCCCT CGGCTCGGGG CCCCTTCCTC CTTCACAGCC ACAGCGCAGC CTCTTCCAGT 
CAGTCTCTCA CCTCCCACCG CCTGTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LONP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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