UBE4A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE4A
Entrez Gene ID	9354
Full Name	ubiquitination factor E4A
Synonyms	E4,UBOX2,UFD2
General protein information	Preferred Names ubiquitination factor E4A Names ubiquitin conjugation factor E4 A RING-type E3 ubiquitin transferase E4 A ubiquitination factor E4A (UFD2 homolog, yeast)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.3
Summary	This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011].
Disorder MIM:	603753

mRNA and Protein(s)

mRNA	Protein	Name
NM_004788.3	NP_004779.2	ubiquitin conjugation factor E4 A isoform 1
NM_001204077.2	NP_001191006.1	ubiquitin conjugation factor E4 A isoform 2
NM_001204077.1	NP_001191006.1	ubiquitin conjugation factor E4 A isoform 2
NM_004788.4	NP_004779.2	ubiquitin conjugation factor E4 A isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	UBE4A	NP_004779.2
Bos taurus (cattle)	UBE4A	NP_001092469.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001681	XP_321414.5
Pan troglodytes (chimpanzee)	UBE4A	XP_508791.2
Xenopus tropicalis (tropical clawed frog)	ube4a	NP_001163971.1
Canis lupus familiaris (dog)	UBE4A	XP_850081.1
Mus musculus (house mouse)	Ube4a	NP_663375.3
Danio rerio (zebrafish)	ube4a	XP_001922961.2
Drosophila melanogaster (fruit fly)	CG11070	NP_609060.1
	UBE4A	XP_001097911.2
Rattus norvegicus (Norway rat)	Ube4a	NP_997493.1
Gallus gallus (chicken)	UBE4A	NP_001026081.1

Gene Ontology
Bibliography

Related articles in PubMed

Poly r(C) binding protein (PCBP) 1 expression is regulated by the E3 ligase UBE4A in thyroid carcinoma.
Zhang MP, Zhang WS, Tan J, Zhao MH, Lian LJ, Cai J
Bioscience reports37(5)(2017 Oct)

The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI).
Sun Y, Jia X, Gao Q, Liu X, Hou L
IUBMB life69(1)16-21(2017 01)

Genes linked to energy metabolism and immunoregulatory mechanisms are associated with subcutaneous adipose tissue distribution in HIV-infected men.
Irvin MR, Shrestha S, Chen YD, Wiener HW, Haritunians T, Vaughan LK, Tiwari HK, Taylor KD, Scherzer R, Saag MS, Grunfeld C, Rotter JI, Arnett DK
Pharmacogenetics and genomics21(12)798-807(2011 Dec)

Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
Li S, Wang L, Berman M, Kong YY, Dorf ME
Immunity35(3)426-40(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ectopic overexpression of UBE4A, but not UBE3C, in cells was downregulated in vitro migration and invasion in these cells. Cumulatively, our data reveals a novel post-translational regulatory mechanism of regulating ILEI1 expression, a protein required for metastatic progression in prostate cancer cells
Title: The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI).

Identified an intestinal cDNA clone encoding ubiquitination factor E4A (UBE4A), a U-box-type ubiquitin-protein ligase. The prevalence of anti-UBE4A IgG in patients with CD was significantly higher than that in patients with UC or healthy controls
Title: Autoantibodies against ubiquitination factor E4A (UBE4A) are associated with severity of Crohn's disease.

UBE4A was over-expressed in ovarian serous cystadenocarcinoma tissues, suggesting that the activity of ubiquitin-proteasome system is enhanced in ovarian cancer.
Title: [Differential expression of USP2, USP14 and UBE4A between ovarian serous cystadenocarcinoma and adjacent normal tissues].

In these studies, we define Ufd2 (a novel E4 polyubiquitylating enzyme) as a new autoantigen in scleroderma, and show that it regulates chromosome condensation and separation during mitosis in human cells.
Title: Ufd2, a novel autoantigen in scleroderma, regulates sister chromatid separation.

UBE4A is expressed in different tissues in a pattern that seems to be dependent from cell type and cell cycle and that UBE4A might have a specific role in different biochemical processes other than ubiquitination, including growth or differentiation.
Title: Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues.

The following UBE4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11624	NM_001204077.2 Latest version!	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu10856	NM_004788.4 Latest version!	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu11624	NM_001204077.1	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu10856	NM_004788.3	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE4A ( NM_001204077.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191006.1 Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11624
Clone ID Related Accession (Same CDS sequence)	NM_001204077.2 , NM_001204077.1
Accession Version	NM_001204077.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	ubiquitin conjugation factor E4 A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068702.1, BC112367.1, AP001267.4, BC039240.1 and AW021567.1. On Jun 2, 2019 this sequence version replaced NM_001204077.1. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.98114.1, SRR1803614.251633.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTCATTG AAGCCTTGAT ATTGGATGAG 
GAAGTTAGAA CATTTCCAGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AGCTCTGTCA GATCCTTTTG TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAAA AAGATATGGC AAAGGTTTTT GTAGAATACA TTCAGCCCAA GGACCCTACC 
AATGGGCAAA TGTACCAGAA GACCTTGCTG GGAGTAATTC TGAGTATCTC CTGCTTATTA 
AAGACTCCGG GTGTTGTAGA AAATCATGGC TACTTTTTGA ATCCATCTCG TTCCAGCCCC 
CAGGAGATCA AAGTACAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCACGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTTTTCCA AATGTATGCC TCAGATGCTT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACAT ACTGTGCCCT CAAGGAGTTG AATGATGAAG AACGAAAAAT TAAAAATGTA 
CACATGAGAG GTTTGGACAA AGAAACCTGT TTGATCCCAG CTGTGCAGGA GCCGAAGTTT 
CCACAGAACT ACAACCTTGT AACAGAGAAC CTTGCTCTGA CAGAGTACAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAATCT TCGTGAGCAG 
TTTGAACGAC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAAATG 
CTACAAAACT GCCTAAACTT GCAGGTGTCC ATGGCTGTTC TACTGGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAGCTA ACCTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACCTGGGTG ATTTTCTCAT TTTTCTCCGC 
CGCTTTGCCG ATGACATTTT GGAGACATCA GCAGATTCCC TGGAGCATGT CCTTCACTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGA ATGAAGAATC CCCACCTGAG GGCCAAACTA 
GCAGAGGTGT TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCAGT ATGCACCCCA ACTTGCAGAG 
GCTCTAATCA AGGTTTTTGT GGACATCGAA TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGCCGTCC CATGTATCCT ATCCTAAGAT ACATGTGGGG GACAGATACC 
TATCGGGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGCTT TCTTAACCTG CTAATGAATG ATGCCATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAAA TTGAGAAGGA TCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAAAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TCGGTACCCT TGCCTTTCTC 
ACATCAGAGA TCAAGTCACT CTTTGTGCAT CCCTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAACA CCTGGTTGGC CCCAAGATGG GTGCCTTAAA AGTCAAGGAC 
TTCAGCGAAT TTGACTTCAA ACCCCAGCAG CTTGTATCAG ATATCTGCAC TATCTACTTA 
AATCTTGGGG ATGAGGAGAA TTTCTGTGCC ACTGTGCCCA AGGATGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTTCGAGTC TTGAAGAAAA TAAATAAGCC TGGGAATATG 
ATTATGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TTGCAGACCT CCAACAACAG 
GAAGAGGAAA CCTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATTAT GAGCACACTG 
ATGTGTGACC CTGTGGTGCT GCCATCTTCC AGAGTCACTG TGGATAGATC CACCATTGCA 
AGACATTTGC TCAGTGACCA AACAGATCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGGT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191006.1
CDS	117..3317
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	NM_001204077.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204077.2

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTCATTG AAGCCTTGAT ATTGGATGAG 
GAAGTTAGAA CATTTCCAGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AGCTCTGTCA GATCCTTTTG TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAAA AAGATATGGC AAAGGTTTTT GTAGAATACA TTCAGCCCAA GGACCCTACC 
AATGGGCAAA TGTACCAGAA GACCTTGCTG GGAGTAATTC TGAGTATCTC CTGCTTATTA 
AAGACTCCGG GTGTTGTAGA AAATCATGGC TACTTTTTGA ATCCATCTCG TTCCAGCCCC 
CAGGAGATCA AAGTACAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCACGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTTTTCCA AATGTATGCC TCAGATGCTT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACAT ACTGTGCCCT CAAGGAGTTG AATGATGAAG AACGAAAAAT TAAAAATGTA 
CACATGAGAG GTTTGGACAA AGAAACCTGT TTGATCCCAG CTGTGCAGGA GCCGAAGTTT 
CCACAGAACT ACAACCTTGT AACAGAGAAC CTTGCTCTGA CAGAGTACAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAATCT TCGTGAGCAG 
TTTGAACGAC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAAATG 
CTACAAAACT GCCTAAACTT GCAGGTGTCC ATGGCTGTTC TACTGGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAGCTA ACCTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACCTGGGTG ATTTTCTCAT TTTTCTCCGC 
CGCTTTGCCG ATGACATTTT GGAGACATCA GCAGATTCCC TGGAGCATGT CCTTCACTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGA ATGAAGAATC CCCACCTGAG GGCCAAACTA 
GCAGAGGTGT TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCAGT ATGCACCCCA ACTTGCAGAG 
GCTCTAATCA AGGTTTTTGT GGACATCGAA TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGCCGTCC CATGTATCCT ATCCTAAGAT ACATGTGGGG GACAGATACC 
TATCGGGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGCTT TCTTAACCTG CTAATGAATG ATGCCATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAAA TTGAGAAGGA TCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAAAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TCGGTACCCT TGCCTTTCTC 
ACATCAGAGA TCAAGTCACT CTTTGTGCAT CCCTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAACA CCTGGTTGGC CCCAAGATGG GTGCCTTAAA AGTCAAGGAC 
TTCAGCGAAT TTGACTTCAA ACCCCAGCAG CTTGTATCAG ATATCTGCAC TATCTACTTA 
AATCTTGGGG ATGAGGAGAA TTTCTGTGCC ACTGTGCCCA AGGATGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTTCGAGTC TTGAAGAAAA TAAATAAGCC TGGGAATATG 
ATTATGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TTGCAGACCT CCAACAACAG 
GAAGAGGAAA CCTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATTAT GAGCACACTG 
ATGTGTGACC CTGTGGTGCT GCCATCTTCC AGAGTCACTG TGGATAGATC CACCATTGCA 
AGACATTTGC TCAGTGACCA AACAGATCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGGT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( NM_001204077.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001191006.1 Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11624
Clone ID Related Accession (Same CDS sequence)	NM_001204077.2 , NM_001204077.1
Accession Version	NM_001204077.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ubiquitin conjugation factor E4 A isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068702.1, BC112367.1, AP001267.4, BC039240.1 and AW021567.1. Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.67026.1, SRR1803611.215878.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTCATTG AAGCCTTGAT ATTGGATGAG 
GAAGTTAGAA CATTTCCAGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AGCTCTGTCA GATCCTTTTG TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAAA AAGATATGGC AAAGGTTTTT GTAGAATACA TTCAGCCCAA GGACCCTACC 
AATGGGCAAA TGTACCAGAA GACCTTGCTG GGAGTAATTC TGAGTATCTC CTGCTTATTA 
AAGACTCCGG GTGTTGTAGA AAATCATGGC TACTTTTTGA ATCCATCTCG TTCCAGCCCC 
CAGGAGATCA AAGTACAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCACGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTTTTCCA AATGTATGCC TCAGATGCTT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACAT ACTGTGCCCT CAAGGAGTTG AATGATGAAG AACGAAAAAT TAAAAATGTA 
CACATGAGAG GTTTGGACAA AGAAACCTGT TTGATCCCAG CTGTGCAGGA GCCGAAGTTT 
CCACAGAACT ACAACCTTGT AACAGAGAAC CTTGCTCTGA CAGAGTACAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAATCT TCGTGAGCAG 
TTTGAACGAC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAAATG 
CTACAAAACT GCCTAAACTT GCAGGTGTCC ATGGCTGTTC TACTGGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAGCTA ACCTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACCTGGGTG ATTTTCTCAT TTTTCTCCGC 
CGCTTTGCCG ATGACATTTT GGAGACATCA GCAGATTCCC TGGAGCATGT CCTTCACTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGA ATGAAGAATC CCCACCTGAG GGCCAAACTA 
GCAGAGGTGT TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCAGT ATGCACCCCA ACTTGCAGAG 
GCTCTAATCA AGGTTTTTGT GGACATCGAA TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGCCGTCC CATGTATCCT ATCCTAAGAT ACATGTGGGG GACAGATACC 
TATCGGGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGCTT TCTTAACCTG CTAATGAATG ATGCCATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAAA TTGAGAAGGA TCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAAAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TCGGTACCCT TGCCTTTCTC 
ACATCAGAGA TCAAGTCACT CTTTGTGCAT CCCTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAACA CCTGGTTGGC CCCAAGATGG GTGCCTTAAA AGTCAAGGAC 
TTCAGCGAAT TTGACTTCAA ACCCCAGCAG CTTGTATCAG ATATCTGCAC TATCTACTTA 
AATCTTGGGG ATGAGGAGAA TTTCTGTGCC ACTGTGCCCA AGGATGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTTCGAGTC TTGAAGAAAA TAAATAAGCC TGGGAATATG 
ATTATGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TTGCAGACCT CCAACAACAG 
GAAGAGGAAA CCTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATTAT GAGCACACTG 
ATGTGTGACC CTGTGGTGCT GCCATCTTCC AGAGTCACTG TGGATAGATC CACCATTGCA 
AGACATTTGC TCAGTGACCA AACAGATCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGGT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001191006.1
CDS	136..3336
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAHFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	NM_001204077.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001204077.1

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CATTTTGAAG ATGTAACTGA GTTTCTGGAA GAGGTCATTG AAGCCTTGAT ATTGGATGAG 
GAAGTTAGAA CATTTCCAGA AGTCATGATT CCAGTGTTTG ATATTTTATT GGGCCGAATA 
AAAGATCTAG AGCTCTGTCA GATCCTTTTG TATGCATATC TGGATATTCT TCTCTATTTC 
ACTAGGCAAA AAGATATGGC AAAGGTTTTT GTAGAATACA TTCAGCCCAA GGACCCTACC 
AATGGGCAAA TGTACCAGAA GACCTTGCTG GGAGTAATTC TGAGTATCTC CTGCTTATTA 
AAGACTCCGG GTGTTGTAGA AAATCATGGC TACTTTTTGA ATCCATCTCG TTCCAGCCCC 
CAGGAGATCA AAGTACAGGA GGCCAACATC CATCAGTTCA TGGCTCAGTT CCACGAAAAG 
ATCTACCAGA TGCTGAAGAA CTTACTCCAG CTCTCTCCAG AAACCAAACA CTGTATCTTG 
TCCTGGCTTG GAAACTGTTT GCATGCAAAT GCAGGCCGCA CCAAGATTTG GGCCAATCAG 
ATGCCAGAAA TCTTTTTCCA AATGTATGCC TCAGATGCTT TCTTTCTGAA TCTGGGTGCT 
GCTCTCCTGA AGCTATGCCA GCCATTTTGC AAACCCAGAT CCTCTCGGCT CCTCACCTTT 
AATCCCACAT ACTGTGCCCT CAAGGAGTTG AATGATGAAG AACGAAAAAT TAAAAATGTA 
CACATGAGAG GTTTGGACAA AGAAACCTGT TTGATCCCAG CTGTGCAGGA GCCGAAGTTT 
CCACAGAACT ACAACCTTGT AACAGAGAAC CTTGCTCTGA CAGAGTACAC CTTGTACTTG 
GGATTTCACA GGTTGCATGA TCAGATGGTA AAAATCAACC AAAATCTGCA TCGGCTGCAG 
GTTGCCTGGC GGGATGCTCA GCAAAGTTCT AGCCCTGCTG CTGACAATCT TCGTGAGCAG 
TTTGAACGAC TGATGACCAT CTATCTTTCT ACCAAGACTG CCATGACAGA GCCACAAATG 
CTACAAAACT GCCTAAACTT GCAGGTGTCC ATGGCTGTTC TACTGGTTCA ACTGGCCATA 
GGCAATGAGG GCTCACAGCC AATAGAGCTA ACCTTTCCTT TGCCAGATGG CTACAGCTCT 
TTGGCTTATG TGCCAGAATT TTTTGCAGAT AACCTGGGTG ATTTTCTCAT TTTTCTCCGC 
CGCTTTGCCG ATGACATTTT GGAGACATCA GCAGATTCCC TGGAGCATGT CCTTCACTTT 
ATCACCATTT TCACTGGAAG CATAGAAAGA ATGAAGAATC CCCACCTGAG GGCCAAACTA 
GCAGAGGTGT TGGAAGCAGT GATGCCCCAC CTGGATCAGA CCCCAAATCC CTTGGTATCC 
AGTGTGTTCC ACCGGAAACG TGTGTTCTGC AACTTTCAGT ATGCACCCCA ACTTGCAGAG 
GCTCTAATCA AGGTTTTTGT GGACATCGAA TTTACAGGAG ACCCCCATCA ATTTGAACAG 
AAGTTTAATT ACCGCCGTCC CATGTATCCT ATCCTAAGAT ACATGTGGGG GACAGATACC 
TATCGGGAGA GCATTAAGGA TTTGGCTGAC TATGCCTCTA AGAATTTAGA AGCCATGAAT 
CCCCCACTTT TCCTCCGCTT TCTTAACCTG CTAATGAATG ATGCCATCTT CCTTTTGGAT 
GAAGCCATAC AGTATTTGAG CAAGATAAAG ATTCAGCAAA TTGAGAAGGA TCGAGGTGAA 
TGGGATAGTC TGACTCCAGA AGCCCGCCGA GAAAAGGAGG CTGGCCTACA GATGTTTGGA 
CAGCTGGCAC GTTTCCATAA CATCATGTCC AATGAAACAA TCGGTACCCT TGCCTTTCTC 
ACATCAGAGA TCAAGTCACT CTTTGTGCAT CCCTTCCTGG CTGAGCGCAT CATCTCCATG 
TTGAACTACT TCCTGCAACA CCTGGTTGGC CCCAAGATGG GTGCCTTAAA AGTCAAGGAC 
TTCAGCGAAT TTGACTTCAA ACCCCAGCAG CTTGTATCAG ATATCTGCAC TATCTACTTA 
AATCTTGGGG ATGAGGAGAA TTTCTGTGCC ACTGTGCCCA AGGATGGACG TTCCTATTCC 
CCAACTCTCT TTGCACAGAC AGTTCGAGTC TTGAAGAAAA TAAATAAGCC TGGGAATATG 
ATTATGGCTT TCAGCAACTT GGCAGAGAGA ATCAAGTCTC TTGCAGACCT CCAACAACAG 
GAAGAGGAAA CCTATGCAGA TGCCTGTGAT GAGTTCCTGG ATCCCATTAT GAGCACACTG 
ATGTGTGACC CTGTGGTGCT GCCATCTTCC AGAGTCACTG TGGATAGATC CACCATTGCA 
AGACATTTGC TCAGTGACCA AACAGATCCC TTTAACCGTA GTCCCCTCAC CATGGACCAG 
ATCCGGCCAA ACACAGAACT AAAAGAAAAA ATCCAACGGT GGCTTGCAGA GAGGAAACAA 
CAAAAGGAGC AACTTGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( NM_004788.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004779.2 Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10856
Clone ID Related Accession (Same CDS sequence)	NM_004788.3 , NM_004788.4
Accession Version	NM_004788.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	ubiquitin conjugation factor E4 A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068702.1, BC062695.1, D50916.1, AP001267.4, BC039240.1 and AW021567.1. This sequence is a reference standard in the RefSeqGene project. On Feb 12, 2011 this sequence version replaced NM_004788.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: D50916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CGTGAGTACA TGAACAAGAT CTATTTTGAA GATGTAACTG AGTTTCTGGA AGAGGTCATT 
GAAGCCTTGA TATTGGATGA GGAAGTTAGA ACATTTCCAG AAGTCATGAT TCCAGTGTTT 
GATATTTTAT TGGGCCGAAT AAAAGATCTA GAGCTCTGTC AGATCCTTTT GTATGCATAT 
CTGGATATTC TTCTCTATTT CACTAGGCAA AAAGATATGG CAAAGGTTTT TGTAGAATAC 
ATTCAGCCCA AGGACCCTAC CAATGGGCAA ATGTACCAGA AGACCTTGCT GGGAGTAATT 
CTGAGTATCT CCTGCTTATT AAAGACTCCG GGTGTTGTAG AAAATCATGG CTACTTTTTG 
AATCCATCTC GTTCCAGCCC CCAGGAGATC AAAGTACAGG AGGCCAACAT CCATCAGTTC 
ATGGCTCAGT TCCACGAAAA GATCTACCAG ATGCTGAAGA ACTTACTCCA GCTCTCTCCA 
GAAACCAAAC ACTGTATCTT GTCCTGGCTT GGAAACTGTT TGCATGCAAA TGCAGGCCGC 
ACCAAGATTT GGGCCAATCA GATGCCAGAA ATCTTTTTCC AAATGTATGC CTCAGATGCT 
TTCTTTCTGA ATCTGGGTGC TGCTCTCCTG AAGCTATGCC AGCCATTTTG CAAACCCAGA 
TCCTCTCGGC TCCTCACCTT TAATCCCACA TACTGTGCCC TCAAGGAGTT GAATGATGAA 
GAACGAAAAA TTAAAAATGT ACACATGAGA GGTTTGGACA AAGAAACCTG TTTGATCCCA 
GCTGTGCAGG AGCCGAAGTT TCCACAGAAC TACAACCTTG TAACAGAGAA CCTTGCTCTG 
ACAGAGTACA CCTTGTACTT GGGATTTCAC AGGTTGCATG ATCAGATGGT AAAAATCAAC 
CAAAATCTGC ATCGGCTGCA GGTTGCCTGG CGGGATGCTC AGCAAAGTTC TAGCCCTGCT 
GCTGACAATC TTCGTGAGCA GTTTGAACGA CTGATGACCA TCTATCTTTC TACCAAGACT 
GCCATGACAG AGCCACAAAT GCTACAAAAC TGCCTAAACT TGCAGGTGTC CATGGCTGTT 
CTACTGGTTC AACTGGCCAT AGGCAATGAG GGCTCACAGC CAATAGAGCT AACCTTTCCT 
TTGCCAGATG GCTACAGCTC TTTGGCTTAT GTGCCAGAAT TTTTTGCAGA TAACCTGGGT 
GATTTTCTCA TTTTTCTCCG CCGCTTTGCC GATGACATTT TGGAGACATC AGCAGATTCC 
CTGGAGCATG TCCTTCACTT TATCACCATT TTCACTGGAA GCATAGAAAG AATGAAGAAT 
CCCCACCTGA GGGCCAAACT AGCAGAGGTG TTGGAAGCAG TGATGCCCCA CCTGGATCAG 
ACCCCAAATC CCTTGGTATC CAGTGTGTTC CACCGGAAAC GTGTGTTCTG CAACTTTCAG 
TATGCACCCC AACTTGCAGA GGCTCTAATC AAGGTTTTTG TGGACATCGA ATTTACAGGA 
GACCCCCATC AATTTGAACA GAAGTTTAAT TACCGCCGTC CCATGTATCC TATCCTAAGA 
TACATGTGGG GGACAGATAC CTATCGGGAG AGCATTAAGG ATTTGGCTGA CTATGCCTCT 
AAGAATTTAG AAGCCATGAA TCCCCCACTT TTCCTCCGCT TTCTTAACCT GCTAATGAAT 
GATGCCATCT TCCTTTTGGA TGAAGCCATA CAGTATTTGA GCAAGATAAA GATTCAGCAA 
ATTGAGAAGG ATCGAGGTGA ATGGGATAGT CTGACTCCAG AAGCCCGCCG AGAAAAGGAG 
GCTGGCCTAC AGATGTTTGG ACAGCTGGCA CGTTTCCATA ACATCATGTC CAATGAAACA 
ATCGGTACCC TTGCCTTTCT CACATCAGAG ATCAAGTCAC TCTTTGTGCA TCCCTTCCTG 
GCTGAGCGCA TCATCTCCAT GTTGAACTAC TTCCTGCAAC ACCTGGTTGG CCCCAAGATG 
GGTGCCTTAA AAGTCAAGGA CTTCAGCGAA TTTGACTTCA AACCCCAGCA GCTTGTATCA 
GATATCTGCA CTATCTACTT AAATCTTGGG GATGAGGAGA ATTTCTGTGC CACTGTGCCC 
AAGGATGGAC GTTCCTATTC CCCAACTCTC TTTGCACAGA CAGTTCGAGT CTTGAAGAAA 
ATAAATAAGC CTGGGAATAT GATTATGGCT TTCAGCAACT TGGCAGAGAG AATCAAGTCT 
CTTGCAGACC TCCAACAACA GGAAGAGGAA ACCTATGCAG ATGCCTGTGA TGAGTTCCTG 
GATCCCATTA TGAGCACACT GATGTGTGAC CCTGTGGTGC TGCCATCTTC CAGAGTCACT 
GTGGATAGAT CCACCATTGC AAGACATTTG CTCAGTGACC AAACAGATCC CTTTAACCGT 
AGTCCCCTCA CCATGGACCA GATCCGGCCA AACACAGAAC TAAAAGAAAA AATCCAACGG 
TGGCTTGCAG AGAGGAAACA ACAAAAGGAG CAACTTGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004779.2
CDS	136..3357
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	NM_004788.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004788.3

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CGTGAGTACA TGAACAAGAT CTATTTTGAA GATGTAACTG AGTTTCTGGA AGAGGTCATT 
GAAGCCTTGA TATTGGATGA GGAAGTTAGA ACATTTCCAG AAGTCATGAT TCCAGTGTTT 
GATATTTTAT TGGGCCGAAT AAAAGATCTA GAGCTCTGTC AGATCCTTTT GTATGCATAT 
CTGGATATTC TTCTCTATTT CACTAGGCAA AAAGATATGG CAAAGGTTTT TGTAGAATAC 
ATTCAGCCCA AGGACCCTAC CAATGGGCAA ATGTACCAGA AGACCTTGCT GGGAGTAATT 
CTGAGTATCT CCTGCTTATT AAAGACTCCG GGTGTTGTAG AAAATCATGG CTACTTTTTG 
AATCCATCTC GTTCCAGCCC CCAGGAGATC AAAGTACAGG AGGCCAACAT CCATCAGTTC 
ATGGCTCAGT TCCACGAAAA GATCTACCAG ATGCTGAAGA ACTTACTCCA GCTCTCTCCA 
GAAACCAAAC ACTGTATCTT GTCCTGGCTT GGAAACTGTT TGCATGCAAA TGCAGGCCGC 
ACCAAGATTT GGGCCAATCA GATGCCAGAA ATCTTTTTCC AAATGTATGC CTCAGATGCT 
TTCTTTCTGA ATCTGGGTGC TGCTCTCCTG AAGCTATGCC AGCCATTTTG CAAACCCAGA 
TCCTCTCGGC TCCTCACCTT TAATCCCACA TACTGTGCCC TCAAGGAGTT GAATGATGAA 
GAACGAAAAA TTAAAAATGT ACACATGAGA GGTTTGGACA AAGAAACCTG TTTGATCCCA 
GCTGTGCAGG AGCCGAAGTT TCCACAGAAC TACAACCTTG TAACAGAGAA CCTTGCTCTG 
ACAGAGTACA CCTTGTACTT GGGATTTCAC AGGTTGCATG ATCAGATGGT AAAAATCAAC 
CAAAATCTGC ATCGGCTGCA GGTTGCCTGG CGGGATGCTC AGCAAAGTTC TAGCCCTGCT 
GCTGACAATC TTCGTGAGCA GTTTGAACGA CTGATGACCA TCTATCTTTC TACCAAGACT 
GCCATGACAG AGCCACAAAT GCTACAAAAC TGCCTAAACT TGCAGGTGTC CATGGCTGTT 
CTACTGGTTC AACTGGCCAT AGGCAATGAG GGCTCACAGC CAATAGAGCT AACCTTTCCT 
TTGCCAGATG GCTACAGCTC TTTGGCTTAT GTGCCAGAAT TTTTTGCAGA TAACCTGGGT 
GATTTTCTCA TTTTTCTCCG CCGCTTTGCC GATGACATTT TGGAGACATC AGCAGATTCC 
CTGGAGCATG TCCTTCACTT TATCACCATT TTCACTGGAA GCATAGAAAG AATGAAGAAT 
CCCCACCTGA GGGCCAAACT AGCAGAGGTG TTGGAAGCAG TGATGCCCCA CCTGGATCAG 
ACCCCAAATC CCTTGGTATC CAGTGTGTTC CACCGGAAAC GTGTGTTCTG CAACTTTCAG 
TATGCACCCC AACTTGCAGA GGCTCTAATC AAGGTTTTTG TGGACATCGA ATTTACAGGA 
GACCCCCATC AATTTGAACA GAAGTTTAAT TACCGCCGTC CCATGTATCC TATCCTAAGA 
TACATGTGGG GGACAGATAC CTATCGGGAG AGCATTAAGG ATTTGGCTGA CTATGCCTCT 
AAGAATTTAG AAGCCATGAA TCCCCCACTT TTCCTCCGCT TTCTTAACCT GCTAATGAAT 
GATGCCATCT TCCTTTTGGA TGAAGCCATA CAGTATTTGA GCAAGATAAA GATTCAGCAA 
ATTGAGAAGG ATCGAGGTGA ATGGGATAGT CTGACTCCAG AAGCCCGCCG AGAAAAGGAG 
GCTGGCCTAC AGATGTTTGG ACAGCTGGCA CGTTTCCATA ACATCATGTC CAATGAAACA 
ATCGGTACCC TTGCCTTTCT CACATCAGAG ATCAAGTCAC TCTTTGTGCA TCCCTTCCTG 
GCTGAGCGCA TCATCTCCAT GTTGAACTAC TTCCTGCAAC ACCTGGTTGG CCCCAAGATG 
GGTGCCTTAA AAGTCAAGGA CTTCAGCGAA TTTGACTTCA AACCCCAGCA GCTTGTATCA 
GATATCTGCA CTATCTACTT AAATCTTGGG GATGAGGAGA ATTTCTGTGC CACTGTGCCC 
AAGGATGGAC GTTCCTATTC CCCAACTCTC TTTGCACAGA CAGTTCGAGT CTTGAAGAAA 
ATAAATAAGC CTGGGAATAT GATTATGGCT TTCAGCAACT TGGCAGAGAG AATCAAGTCT 
CTTGCAGACC TCCAACAACA GGAAGAGGAA ACCTATGCAG ATGCCTGTGA TGAGTTCCTG 
GATCCCATTA TGAGCACACT GATGTGTGAC CCTGTGGTGC TGCCATCTTC CAGAGTCACT 
GTGGATAGAT CCACCATTGC AAGACATTTG CTCAGTGACC AAACAGATCC CTTTAACCGT 
AGTCCCCTCA CCATGGACCA GATCCGGCCA AACACAGAAC TAAAAGAAAA AATCCAACGG 
TGGCTTGCAG AGAGGAAACA ACAAAAGGAG CAACTTGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE4A ( NM_004788.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004779.2 Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10856
Clone ID Related Accession (Same CDS sequence)	NM_004788.3 , NM_004788.4
Accession Version	NM_004788.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	ubiquitin conjugation factor E4 A isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068702.1, BC062695.1, D50916.1, AP001267.4, BC039240.1 and AW021567.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_004788.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). ##Evidence-Data-START## Transcript exon combination :: D50916.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CGTGAGTACA TGAACAAGAT CTATTTTGAA GATGTAACTG AGTTTCTGGA AGAGGTCATT 
GAAGCCTTGA TATTGGATGA GGAAGTTAGA ACATTTCCAG AAGTCATGAT TCCAGTGTTT 
GATATTTTAT TGGGCCGAAT AAAAGATCTA GAGCTCTGTC AGATCCTTTT GTATGCATAT 
CTGGATATTC TTCTCTATTT CACTAGGCAA AAAGATATGG CAAAGGTTTT TGTAGAATAC 
ATTCAGCCCA AGGACCCTAC CAATGGGCAA ATGTACCAGA AGACCTTGCT GGGAGTAATT 
CTGAGTATCT CCTGCTTATT AAAGACTCCG GGTGTTGTAG AAAATCATGG CTACTTTTTG 
AATCCATCTC GTTCCAGCCC CCAGGAGATC AAAGTACAGG AGGCCAACAT CCATCAGTTC 
ATGGCTCAGT TCCACGAAAA GATCTACCAG ATGCTGAAGA ACTTACTCCA GCTCTCTCCA 
GAAACCAAAC ACTGTATCTT GTCCTGGCTT GGAAACTGTT TGCATGCAAA TGCAGGCCGC 
ACCAAGATTT GGGCCAATCA GATGCCAGAA ATCTTTTTCC AAATGTATGC CTCAGATGCT 
TTCTTTCTGA ATCTGGGTGC TGCTCTCCTG AAGCTATGCC AGCCATTTTG CAAACCCAGA 
TCCTCTCGGC TCCTCACCTT TAATCCCACA TACTGTGCCC TCAAGGAGTT GAATGATGAA 
GAACGAAAAA TTAAAAATGT ACACATGAGA GGTTTGGACA AAGAAACCTG TTTGATCCCA 
GCTGTGCAGG AGCCGAAGTT TCCACAGAAC TACAACCTTG TAACAGAGAA CCTTGCTCTG 
ACAGAGTACA CCTTGTACTT GGGATTTCAC AGGTTGCATG ATCAGATGGT AAAAATCAAC 
CAAAATCTGC ATCGGCTGCA GGTTGCCTGG CGGGATGCTC AGCAAAGTTC TAGCCCTGCT 
GCTGACAATC TTCGTGAGCA GTTTGAACGA CTGATGACCA TCTATCTTTC TACCAAGACT 
GCCATGACAG AGCCACAAAT GCTACAAAAC TGCCTAAACT TGCAGGTGTC CATGGCTGTT 
CTACTGGTTC AACTGGCCAT AGGCAATGAG GGCTCACAGC CAATAGAGCT AACCTTTCCT 
TTGCCAGATG GCTACAGCTC TTTGGCTTAT GTGCCAGAAT TTTTTGCAGA TAACCTGGGT 
GATTTTCTCA TTTTTCTCCG CCGCTTTGCC GATGACATTT TGGAGACATC AGCAGATTCC 
CTGGAGCATG TCCTTCACTT TATCACCATT TTCACTGGAA GCATAGAAAG AATGAAGAAT 
CCCCACCTGA GGGCCAAACT AGCAGAGGTG TTGGAAGCAG TGATGCCCCA CCTGGATCAG 
ACCCCAAATC CCTTGGTATC CAGTGTGTTC CACCGGAAAC GTGTGTTCTG CAACTTTCAG 
TATGCACCCC AACTTGCAGA GGCTCTAATC AAGGTTTTTG TGGACATCGA ATTTACAGGA 
GACCCCCATC AATTTGAACA GAAGTTTAAT TACCGCCGTC CCATGTATCC TATCCTAAGA 
TACATGTGGG GGACAGATAC CTATCGGGAG AGCATTAAGG ATTTGGCTGA CTATGCCTCT 
AAGAATTTAG AAGCCATGAA TCCCCCACTT TTCCTCCGCT TTCTTAACCT GCTAATGAAT 
GATGCCATCT TCCTTTTGGA TGAAGCCATA CAGTATTTGA GCAAGATAAA GATTCAGCAA 
ATTGAGAAGG ATCGAGGTGA ATGGGATAGT CTGACTCCAG AAGCCCGCCG AGAAAAGGAG 
GCTGGCCTAC AGATGTTTGG ACAGCTGGCA CGTTTCCATA ACATCATGTC CAATGAAACA 
ATCGGTACCC TTGCCTTTCT CACATCAGAG ATCAAGTCAC TCTTTGTGCA TCCCTTCCTG 
GCTGAGCGCA TCATCTCCAT GTTGAACTAC TTCCTGCAAC ACCTGGTTGG CCCCAAGATG 
GGTGCCTTAA AAGTCAAGGA CTTCAGCGAA TTTGACTTCA AACCCCAGCA GCTTGTATCA 
GATATCTGCA CTATCTACTT AAATCTTGGG GATGAGGAGA ATTTCTGTGC CACTGTGCCC 
AAGGATGGAC GTTCCTATTC CCCAACTCTC TTTGCACAGA CAGTTCGAGT CTTGAAGAAA 
ATAAATAAGC CTGGGAATAT GATTATGGCT TTCAGCAACT TGGCAGAGAG AATCAAGTCT 
CTTGCAGACC TCCAACAACA GGAAGAGGAA ACCTATGCAG ATGCCTGTGA TGAGTTCCTG 
GATCCCATTA TGAGCACACT GATGTGTGAC CCTGTGGTGC TGCCATCTTC CAGAGTCACT 
GTGGATAGAT CCACCATTGC AAGACATTTG CTCAGTGACC AAACAGATCC CTTTAACCGT 
AGTCCCCTCA CCATGGACCA GATCCGGCCA AACACAGAAC TAAAAGAAAA AATCCAACGG 
TGGCTTGCAG AGAGGAAACA ACAAAAGGAG CAACTTGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004779.2
CDS	117..3338
Translation	MTDQENNNNISSNPFAALFGSLADAKQFAAIQKEQLKQQSDELPASPDDSDNSVSESLDEFDYSVAEISRSFRSQQEICEQLNINHMIQRIFLITLDNSDPSLKSGNGIPSRCVYLEEMAVELEDQDWLDMSNVEQALFARLLLQDPGNHLINMTSSTTLNLSADRDAGERHIFCYLYSCFQRAKEEITKVPENLLPFAVQCRNLTVSNTRTVLLTPEIYVDQNIHEQLVDLMLEAIQGAREYMNKIYFEDVTEFLEEVIEALILDEEVRTFPEVMIPVFDILLGRIKDLELCQILLYAYLDILLYFTRQKDMAKVFVEYIQPKDPTNGQMYQKTLLGVILSISCLLKTPGVVENHGYFLNPSRSSPQEIKVQEANIHQFMAQFHEKIYQMLKNLLQLSPETKHCILSWLGNCLHANAGRTKIWANQMPEIFFQMYASDAFFLNLGAALLKLCQPFCKPRSSRLLTFNPTYCALKELNDEERKIKNVHMRGLDKETCLIPAVQEPKFPQNYNLVTENLALTEYTLYLGFHRLHDQMVKINQNLHRLQVAWRDAQQSSSPAADNLREQFERLMTIYLSTKTAMTEPQMLQNCLNLQVSMAVLLVQLAIGNEGSQPIELTFPLPDGYSSLAYVPEFFADNLGDFLIFLRRFADDILETSADSLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSVFHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKFNYRRPMYPILRYMWGTDTYRESIKDLADYASKNLEAMNPPLFLRFLNLLMNDAIFLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFHNIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGALKVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQTVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPIMSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTELKEKIQRWLAERKQQKEQLE

Target ORF information:

RefSeq Version	NM_004788.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitination factor E4A (UBE4A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004788.4

ORF Insert Sequence:

ATGACAGACC AGGAGAATAA CAACAACATC TCAAGTAACC CCTTTGCTGC TCTTTTTGGC 
TCCCTGGCTG ATGCCAAACA GTTTGCGGCA ATCCAAAAAG AGCAGCTGAA GCAACAATCT 
GATGAACTCC CAGCTAGCCC AGATGACTCG GATAATAGCG TGTCAGAGAG CCTGGATGAA 
TTCGATTACT CTGTGGCTGA GATTAGCCGC TCATTCCGAT CACAGCAGGA AATATGTGAG 
CAACTCAACA TCAATCACAT GATCCAAAGG ATCTTCCTTA TTACTCTGGA CAACAGTGAT 
CCCAGCTTGA AAAGCGGGAA TGGCATCCCT AGCCGTTGTG TGTATTTGGA AGAAATGGCA 
GTAGAGCTAG AAGATCAAGA CTGGCTTGAT ATGAGCAATG TTGAGCAGGC CCTCTTCGCT 
CGCTTATTAC TTCAAGATCC AGGCAACCAC TTAATTAACA TGACTTCTTC TACAACGCTA 
AATCTCTCTG CTGATCGAGA TGCAGGAGAG AGGCACATTT TTTGTTACCT TTACTCCTGC 
TTCCAGAGAG CCAAGGAAGA GATTACCAAA GTTCCAGAGA ACCTGCTACC CTTTGCAGTG 
CAGTGCAGAA ACCTCACTGT GTCCAATACC CGAACAGTTC TTCTCACCCC AGAGATCTAT 
GTTGACCAAA ACATCCATGA GCAACTGGTA GATTTGATGT TAGAAGCCAT CCAGGGAGCC 
CGTGAGTACA TGAACAAGAT CTATTTTGAA GATGTAACTG AGTTTCTGGA AGAGGTCATT 
GAAGCCTTGA TATTGGATGA GGAAGTTAGA ACATTTCCAG AAGTCATGAT TCCAGTGTTT 
GATATTTTAT TGGGCCGAAT AAAAGATCTA GAGCTCTGTC AGATCCTTTT GTATGCATAT 
CTGGATATTC TTCTCTATTT CACTAGGCAA AAAGATATGG CAAAGGTTTT TGTAGAATAC 
ATTCAGCCCA AGGACCCTAC CAATGGGCAA ATGTACCAGA AGACCTTGCT GGGAGTAATT 
CTGAGTATCT CCTGCTTATT AAAGACTCCG GGTGTTGTAG AAAATCATGG CTACTTTTTG 
AATCCATCTC GTTCCAGCCC CCAGGAGATC AAAGTACAGG AGGCCAACAT CCATCAGTTC 
ATGGCTCAGT TCCACGAAAA GATCTACCAG ATGCTGAAGA ACTTACTCCA GCTCTCTCCA 
GAAACCAAAC ACTGTATCTT GTCCTGGCTT GGAAACTGTT TGCATGCAAA TGCAGGCCGC 
ACCAAGATTT GGGCCAATCA GATGCCAGAA ATCTTTTTCC AAATGTATGC CTCAGATGCT 
TTCTTTCTGA ATCTGGGTGC TGCTCTCCTG AAGCTATGCC AGCCATTTTG CAAACCCAGA 
TCCTCTCGGC TCCTCACCTT TAATCCCACA TACTGTGCCC TCAAGGAGTT GAATGATGAA 
GAACGAAAAA TTAAAAATGT ACACATGAGA GGTTTGGACA AAGAAACCTG TTTGATCCCA 
GCTGTGCAGG AGCCGAAGTT TCCACAGAAC TACAACCTTG TAACAGAGAA CCTTGCTCTG 
ACAGAGTACA CCTTGTACTT GGGATTTCAC AGGTTGCATG ATCAGATGGT AAAAATCAAC 
CAAAATCTGC ATCGGCTGCA GGTTGCCTGG CGGGATGCTC AGCAAAGTTC TAGCCCTGCT 
GCTGACAATC TTCGTGAGCA GTTTGAACGA CTGATGACCA TCTATCTTTC TACCAAGACT 
GCCATGACAG AGCCACAAAT GCTACAAAAC TGCCTAAACT TGCAGGTGTC CATGGCTGTT 
CTACTGGTTC AACTGGCCAT AGGCAATGAG GGCTCACAGC CAATAGAGCT AACCTTTCCT 
TTGCCAGATG GCTACAGCTC TTTGGCTTAT GTGCCAGAAT TTTTTGCAGA TAACCTGGGT 
GATTTTCTCA TTTTTCTCCG CCGCTTTGCC GATGACATTT TGGAGACATC AGCAGATTCC 
CTGGAGCATG TCCTTCACTT TATCACCATT TTCACTGGAA GCATAGAAAG AATGAAGAAT 
CCCCACCTGA GGGCCAAACT AGCAGAGGTG TTGGAAGCAG TGATGCCCCA CCTGGATCAG 
ACCCCAAATC CCTTGGTATC CAGTGTGTTC CACCGGAAAC GTGTGTTCTG CAACTTTCAG 
TATGCACCCC AACTTGCAGA GGCTCTAATC AAGGTTTTTG TGGACATCGA ATTTACAGGA 
GACCCCCATC AATTTGAACA GAAGTTTAAT TACCGCCGTC CCATGTATCC TATCCTAAGA 
TACATGTGGG GGACAGATAC CTATCGGGAG AGCATTAAGG ATTTGGCTGA CTATGCCTCT 
AAGAATTTAG AAGCCATGAA TCCCCCACTT TTCCTCCGCT TTCTTAACCT GCTAATGAAT 
GATGCCATCT TCCTTTTGGA TGAAGCCATA CAGTATTTGA GCAAGATAAA GATTCAGCAA 
ATTGAGAAGG ATCGAGGTGA ATGGGATAGT CTGACTCCAG AAGCCCGCCG AGAAAAGGAG 
GCTGGCCTAC AGATGTTTGG ACAGCTGGCA CGTTTCCATA ACATCATGTC CAATGAAACA 
ATCGGTACCC TTGCCTTTCT CACATCAGAG ATCAAGTCAC TCTTTGTGCA TCCCTTCCTG 
GCTGAGCGCA TCATCTCCAT GTTGAACTAC TTCCTGCAAC ACCTGGTTGG CCCCAAGATG 
GGTGCCTTAA AAGTCAAGGA CTTCAGCGAA TTTGACTTCA AACCCCAGCA GCTTGTATCA 
GATATCTGCA CTATCTACTT AAATCTTGGG GATGAGGAGA ATTTCTGTGC CACTGTGCCC 
AAGGATGGAC GTTCCTATTC CCCAACTCTC TTTGCACAGA CAGTTCGAGT CTTGAAGAAA 
ATAAATAAGC CTGGGAATAT GATTATGGCT TTCAGCAACT TGGCAGAGAG AATCAAGTCT 
CTTGCAGACC TCCAACAACA GGAAGAGGAA ACCTATGCAG ATGCCTGTGA TGAGTTCCTG 
GATCCCATTA TGAGCACACT GATGTGTGAC CCTGTGGTGC TGCCATCTTC CAGAGTCACT 
GTGGATAGAT CCACCATTGC AAGACATTTG CTCAGTGACC AAACAGATCC CTTTAACCGT 
AGTCCCCTCA CCATGGACCA GATCCGGCCA AACACAGAAC TAAAAGAAAA AATCCAACGG 
TGGCTTGCAG AGAGGAAACA ACAAAAGGAG CAACTTGAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Poly r(C) binding protein (PCBP) 1 expression is regulated by the E3 ligase UBE4A in thyroid carcinoma.
Bioscience reports37(5)(2017 Oct)
Zhang MP,Zhang WS,Tan J,Zhao MH,Lian LJ,Cai J

The ubiquitin ligase UBE4A inhibits prostate cancer progression by targeting interleukin-like EMT inducer (ILEI).
IUBMB life69(1)16-21(2017 01)
Sun Y,Jia X,Gao Q,Liu X,Hou L

Genes linked to energy metabolism and immunoregulatory mechanisms are associated with subcutaneous adipose tissue distribution in HIV-infected men.
Pharmacogenetics and genomics21(12)798-807(2011 Dec)
Irvin MR,Shrestha S,Chen YD,Wiener HW,Haritunians T,Vaughan LK,Tiwari HK,Taylor KD,Scherzer R,Saag MS,Grunfeld C,Rotter JI,Arnett DK

Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
Immunity35(3)426-40(2011 Sep)
Li S,Wang L,Berman M,Kong YY,Dorf ME

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UBE4A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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