TRIP12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIP12
Entrez Gene ID	9320
Full Name	thyroid hormone receptor interactor 12
Synonyms	MRD49,TRIP-12,ULF
General protein information	Preferred Names thyroid hormone receptor interactor 12 Names E3 ubiquitin-protein ligase TRIP12 E3 ubiquitin-protein ligase for Arf HECT-type E3 ubiquitin transferase TRIP12 TR-interacting protein 12 probable E3 ubiquitin-protein ligase TRIP12 thyroid receptor interacting protein 12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q36.3
Summary	The protein encoded by this gene is an E3 ubiquitin-protein ligase involved in the degradation of the p19ARF/ARF isoform of CDKN2A, a tumor suppressor. The encoded protein also plays a role in the DNA damage response by regulating the stability of USP7, which regulates tumor suppressor p53. [provided by RefSeq, Jan 2017].
Disorder MIM:	604506
Disorder Html:	Mental retardation, autosomal dominant 49, 617752 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011512181.2	XP_011510483.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_017005272.1	XP_016860761.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_017005283.1	XP_016860772.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_017005289.1	XP_016860778.1	E3 ubiquitin-protein ligase TRIP12 isoform X13
XM_017005291.1	XP_016860780.1	E3 ubiquitin-protein ligase TRIP12 isoform X15
NM_001348316.1	NP_001335245.1	E3 ubiquitin-protein ligase TRIP12 isoform b
NM_001348334.1	NP_001335263.1	E3 ubiquitin-protein ligase TRIP12 isoform n
NM_001284215.2	NP_001271144.1	E3 ubiquitin-protein ligase TRIP12 isoform b
NM_001348322.1	NP_001335251.1	E3 ubiquitin-protein ligase TRIP12 isoform h
XM_024453224.1	XP_024308992.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_024453229.1	XP_024308997.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_024453230.1	XP_024308998.1	E3 ubiquitin-protein ligase TRIP12 isoform X2
XM_024453233.1	XP_024309001.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_024453235.1	XP_024309003.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_024453242.1	XP_024309010.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_017005289.2	XP_016860778.1	E3 ubiquitin-protein ligase TRIP12 isoform X11
NM_001348336.1	NP_001335265.1	E3 ubiquitin-protein ligase TRIP12 isoform m
NM_001348333.1	NP_001335262.1	E3 ubiquitin-protein ligase TRIP12 isoform l
NM_001348327.2	NP_001335256.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348324.1	NP_001335253.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348323.1	NP_001335252.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348326.1	NP_001335255.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348315.1	NP_001335244.1	E3 ubiquitin-protein ligase TRIP12 isoform a
NM_001284214.2	NP_001271143.1	E3 ubiquitin-protein ligase TRIP12 isoform a
NM_001348326.2	NP_001335255.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348332.1	NP_001335261.1	E3 ubiquitin-protein ligase TRIP12 isoform k
NM_001348323.2	NP_001335252.1	E3 ubiquitin-protein ligase TRIP12 isoform h
XM_024453225.1	XP_024308993.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_024453231.1	XP_024308999.1	E3 ubiquitin-protein ligase TRIP12 isoform X3
XM_024453237.1	XP_024309005.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_024453241.1	XP_024309009.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_005246955.4	XP_005247012.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_017005293.1	XP_016860782.1	E3 ubiquitin-protein ligase TRIP12 isoform X17
NM_001348330.1	NP_001335259.1	E3 ubiquitin-protein ligase TRIP12 isoform i
NM_001284216.1	NP_001271145.1	E3 ubiquitin-protein ligase TRIP12 isoform d
NM_001348328.1	NP_001335257.1	E3 ubiquitin-protein ligase TRIP12 isoform i
NM_001348331.1	NP_001335260.1	E3 ubiquitin-protein ligase TRIP12 isoform j
XM_024453223.1	XP_024308991.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_024453226.1	XP_024308994.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_024453228.1	XP_024308996.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_024453232.1	XP_024309000.1	E3 ubiquitin-protein ligase TRIP12 isoform X4
XM_024453236.1	XP_024309004.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_024453240.1	XP_024309008.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_017005283.2	XP_016860772.1	E3 ubiquitin-protein ligase TRIP12 isoform X8
XM_017005290.2	XP_016860779.1	E3 ubiquitin-protein ligase TRIP12 isoform X12
XM_024453243.1	XP_024309011.1	E3 ubiquitin-protein ligase TRIP12 isoform X13
NM_004238.3	NP_004229.1	E3 ubiquitin-protein ligase TRIP12 isoform c
NM_001284214.1	NP_001271143.1	E3 ubiquitin-protein ligase TRIP12 isoform a
NM_001348320.1	NP_001335249.1	E3 ubiquitin-protein ligase TRIP12 isoform f
XM_005246960.4	XP_005247017.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_005246961.3	XP_005247018.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_011512183.2	XP_011510485.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
NM_001348329.1	NP_001335258.1	E3 ubiquitin-protein ligase TRIP12 isoform i
NM_001348318.1	NP_001335247.1	E3 ubiquitin-protein ligase TRIP12 isoform e
NM_001284216.2	NP_001271145.1	E3 ubiquitin-protein ligase TRIP12 isoform d
NM_001348320.2	NP_001335249.1	E3 ubiquitin-protein ligase TRIP12 isoform f
NM_001348335.1	NP_001335264.1	E3 ubiquitin-protein ligase TRIP12 isoform m
NM_001348317.1	NP_001335246.1	E3 ubiquitin-protein ligase TRIP12 isoform e
NM_001348318.2	NP_001335247.1	E3 ubiquitin-protein ligase TRIP12 isoform e
NM_001348319.1	NP_001335248.1	E3 ubiquitin-protein ligase TRIP12 isoform f
NM_001348315.2	NP_001335244.1	E3 ubiquitin-protein ligase TRIP12 isoform a
NM_001348316.2	NP_001335245.1	E3 ubiquitin-protein ligase TRIP12 isoform b
NM_001348324.2	NP_001335253.1	E3 ubiquitin-protein ligase TRIP12 isoform h
XM_024453238.1	XP_024309006.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
NM_004238.2	NP_004229.1	E3 ubiquitin-protein ligase TRIP12 isoform c
NM_001348325.1	NP_001335254.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348327.1	NP_001335256.1	E3 ubiquitin-protein ligase TRIP12 isoform h
XM_006712852.3	XP_006712915.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_017005285.1	XP_016860774.1	E3 ubiquitin-protein ligase TRIP12 isoform X10
XM_017005290.1	XP_016860779.1	E3 ubiquitin-protein ligase TRIP12 isoform X14
XM_017005274.1	XP_016860763.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_017005282.1	XP_016860771.1	E3 ubiquitin-protein ligase TRIP12 isoform X7
XM_024453227.1	XP_024308995.1	E3 ubiquitin-protein ligase TRIP12 isoform X1
XM_024453234.1	XP_024309002.1	E3 ubiquitin-protein ligase TRIP12 isoform X5
XM_024453239.1	XP_024309007.1	E3 ubiquitin-protein ligase TRIP12 isoform X6
XM_005246961.4	XP_005247018.1	E3 ubiquitin-protein ligase TRIP12 isoform X9
NM_001348330.2	NP_001335259.1	E3 ubiquitin-protein ligase TRIP12 isoform i
NM_001348329.2	NP_001335258.1	E3 ubiquitin-protein ligase TRIP12 isoform i
NM_001348325.2	NP_001335254.1	E3 ubiquitin-protein ligase TRIP12 isoform h
NM_001348321.1	NP_001335250.1	E3 ubiquitin-protein ligase TRIP12 isoform g

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001296	XP_321851.5
	TRIP12	XP_002799130.1
Mus musculus (house mouse)	Trip12	NP_598736.4
Xenopus tropicalis (tropical clawed frog)	trip12	NP_001136384.1
Homo sapiens (human)	TRIP12	NP_004229.1
Canis lupus familiaris (dog)	TRIP12	XP_005635860.1
Gallus gallus (chicken)	TRIP12	XP_004937121.1
Pan troglodytes (chimpanzee)	TRIP12	XP_001138950.1
Bos taurus (cattle)	TRIP12	NP_001178132.1
Arabidopsis thaliana (thale cress)	KAK	NP_849567.2
Rattus norvegicus (Norway rat)	Trip12	NP_001026829.1
Danio rerio (zebrafish)	trip12	XP_005159284.1
Oryza sativa (rice)	Os02g0101700	NP_001045591.1

Gene Ontology
Bibliography

Related articles in PubMed

Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.
Zhang J, Gambin T, Yuan B, Szafranski P, Rosenfeld JA, Balwi MA, Alswaid A, Al-Gazali L, Shamsi AMA, Komara M, Ali BR, Roeder E, McAuley L, Roy DS, Manchester DK, Magoulas P, King LE, Hannig V, Bonneau D, Denomm?-Pichon AS, Charif M, Besnard T, B?zieau S, Cogn? B, Andrieux J, Zhu W, He W, Vetrini F, Ward PA, Cheung SW, Bi W, Eng CM, Lupski JR, Yang Y, Patel A, Lalani SR, Xia F, Stankiewicz P
Human genetics136(4)377-386(2017 Apr)

TRIP12 as a mediator of human papillomavirus/p16-related radiation enhancement effects.
Wang L, Zhang P, Molkentine DP, Chen C, Molkentine JM, Piao H, Raju U, Zhang J, Valdecanas DR, Tailor RC, Thames HD, Buchholz TA, Chen J, Ma L, Mason KA, Ang KK, Meyn RE, Skinner HD
Oncogene36(6)820-828(2017 Feb)

Identification of new TRIP12 variants and detailed clinical evaluation of individuals with non-syndromic intellectual disability with or without autism.
Bramswig NC, L?decke HJ, Pettersson M, Albrecht B, Bernier RA, Cremer K, Eichler EE, Falkenstein D, Gerdts J, Jansen S, Kuechler A, Kvarnung M, Lindstrand A, Nilsson D, Nordgren A, Pfundt R, Spruijt L, Surowy HM, de Vries BB, Wieland T, Engels H, Strom TM, Kleefstra T, Wieczorek D
Human genetics136(2)179-192(2017 Feb)

Trip12 is an E3 ubiquitin ligase for USP7/HAUSP involved in the DNA damage response.
Liu X, Yang X, Li Y, Zhao S, Li C, Ma P, Mao B
FEBS letters590(23)4213-4222(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

nine presented pathogenic variants further document that TRIP12 haploinsufficiency causes a childhood-onset neurodevelopmental disorder
Title: Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.

p16 overexpression led to downregulation of TRIP12, which in turn led to increased RNF168 levels, repressed DNA damage repair (DDR), increased 53BP1 foci and enhanced radioresponsiveness.
Title: TRIP12 as a mediator of human papillomavirus/p16-related radiation enhancement effects.

We describe the TRIP12-associated phenotype, showing that TRIP12 is a risk gene for non-syndromic intellectual disability with and without autism spectrum disorder, and that TRIP12 mutation carriers present with a broad phenotypic range within the neurodevelopmental phenotypes.
Title: Identification of new TRIP12 variants and detailed clinical evaluation of individuals with non-syndromic intellectual disability with or without autism.

modulatory role for Trip12 in the USP7-dependent DNA damage response
Title: Trip12 is an E3 ubiquitin ligase for USP7/HAUSP involved in the DNA damage response.

Data indicate that E3 ubiquitin ligase thyroid hormone receptor-interacting protein 12 (TRIP12) promotes proteasomal degradation of pancreas transcription factor 1a (PTF1a)and regulates PTF1a activities.
Title: The E3 ubiquitin ligase thyroid hormone receptor-interacting protein 12 targets pancreas transcription factor 1a for proteasomal degradation.

The following TRIP12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIP12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu102295	NM_001348334.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu102326	NM_001348335.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu102326	NM_001348336.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu102230	NM_001348331.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu92734	XM_017005291.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X32, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu07833	NM_001284216.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu08708	NM_004238.3 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu36810	XM_017005293.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X34, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu36810	XM_024453243.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu102139	NM_001348320.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu102139	NM_001348319.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu102192	NM_001348317.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu102192	NM_001348318.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu102232	NM_001348332.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu36804	NM_001348323.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104997	XM_024453223.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104997	XM_024453226.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104997	XM_024453227.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104997	XM_024453225.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104997	XM_024453228.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104997	XM_024453224.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104998	XM_024453229.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104998	XM_024453230.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu104999	XM_024453231.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105000	XM_024453232.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105001	XM_024453233.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105001	XM_024453234.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105001	XM_024453235.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105002	XM_024453239.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105002	XM_024453238.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105002	XM_024453237.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105002	XM_024453240.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105002	XM_024453236.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105003	XM_024453241.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu105004	XM_024453242.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92731	XM_017005283.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92732	XM_017005289.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu92733	XM_017005290.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu08006	NM_001348315.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu08006	NM_001284214.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu08432	NM_001348316.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu08432	NM_001284215.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	NM_001348328.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	NM_001348329.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	NM_001348330.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348322.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348324.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348326.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348325.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348327.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36805	NM_001348321.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36806	NM_001348333.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	XM_017005274.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	XM_011512181.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	XM_005246955.4 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	XM_017005272.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36807	XM_017005282.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36807	XM_005246960.4 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36808	XM_017005285.1 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36808	XM_005246961.4 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36808	XM_006712852.3 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36808	XM_011512183.2 Latest version!	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu92732	XM_017005289.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X30, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu36804	NM_001348326.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348323.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu92731	XM_017005283.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu08006	NM_001284214.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu07833	NM_001284216.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu102139	NM_001348320.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu102192	NM_001348318.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu08006	NM_001348315.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu08432	NM_001348316.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348324.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu08708	NM_004238.2	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu36804	NM_001348327.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu92733	XM_017005290.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X31, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu36808	XM_005246961.3	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	NM_001348330.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36803	NM_001348329.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu36804	NM_001348325.1	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIP12 ( NM_001348334.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335263.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 26, mRNA.

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CloneID	OHu102295
Clone ID Related Accession (Same CDS sequence)	NM_001348334.1
Accession Version	NM_001348334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform n
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10. Transcript Variant: This variant (26) encodes isoform n. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.216433.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGGTAGATT GGTACTTCTT 
AGCTTTAAAA TGCGTTTCTG TCTTTTACAG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335263.1
CDS	166..1401
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGRLVLLSFKMRFCLLQI

Target ORF information:

RefSeq Version	NM_001348334.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348334.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGGTAGATT GGTACTTCTT 
AGCTTTAAAA TGCGTTTCTG TCTTTTACAG ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348336.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335265.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 25, mRNA.

Price & Availability↑

CloneID	OHu102326
Clone ID Related Accession (Same CDS sequence)	NM_001348336.1 , NM_001348335.1
Accession Version	NM_001348336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform m
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10. Transcript Variant: This variant (25), as well as variant 24, encodes isoform m. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.76013.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG GTAGATTGGT ACTTCTTAGC TTTAAAATGC GTTTCTGTCT TTTACAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335265.1
CDS	180..1502
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGRLVLLSFKMRFCLLQI

Target ORF information:

RefSeq Version	NM_001348336.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348336.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG GTAGATTGGT ACTTCTTAGC TTTAAAATGC GTTTCTGTCT TTTACAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348335.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335264.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 24, mRNA.

Price & Availability↑

CloneID	OHu102326
Clone ID Related Accession (Same CDS sequence)	NM_001348336.1 , NM_001348335.1
Accession Version	NM_001348335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform m
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10. Transcript Variant: This variant (24), as well as variant 25, encodes isoform m. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.195876.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG GTAGATTGGT ACTTCTTAGC TTTAAAATGC GTTTCTGTCT TTTACAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335264.1
CDS	83..1405
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGRLVLLSFKMRFCLLQI

Target ORF information:

RefSeq Version	NM_001348335.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348335.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG GTAGATTGGT ACTTCTTAGC TTTAAAATGC GTTTCTGTCT TTTACAGATT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348331.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335260.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 21, mRNA.

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CloneID	OHu102230
Clone ID Related Accession (Same CDS sequence)	NM_001348331.1
Accession Version	NM_001348331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5982bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform j
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005292.1. Transcript Variant: This variant (21) encodes isoform j. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.2740.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAATT AAAATGCCAG TTACAAAGGC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCTCTGG CTTTGGTACA AGCCATCGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGCGATGAC 
GATGGATCAG ATGAGGAAAT AGATGAGTCT CTGGCTGCTC AGTTCCTAAA TTCAGGAAAT 
GTAAGACACA GGCTGCAGTT TTATATTGGA GAACATTTGC TGCCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT TAGTATACAG GCTGAAGATG AAAGAGAATC CACAGATGAT 
GAGAGCAATC CTCTAGGCAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTATAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACAGCTC CAACGAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAC 
GATGGAGTGT GCCCATCAGT ATCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATAA CATTTGAAGA CCCGTCATTA GATGTGATCC TTCTTTTAAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA AATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTAACA GCAAAAGCAA ATAGGCAACT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTTACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGGCAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACCGAGA GGAGCTGCTG 
AAACAGGCGG AGTCTGTGAT GCAGGACCTC GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC GCTTGTATCT 
CAGGAACTAC AGAGAGCTGA CTTGGGTCTT TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGGA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCAAA GCCAGCTCAT ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGC 
TTACCCTTTT ATAAATGGAT GCTACGGCAA GAAACTTCAC TGACATCACA CGATTTGTTT 
GACATCGACC CAGTTGTAGC CAGATCAGTT TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TACAGTATGC ATTAGAAACC 
TTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGACTGG ATTTCACTCT GCCAGGGTTT 
CCCAATATCG AACTGAAGAA AGGAGGGAAG GATATACCAG TCACTATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCTAG GCAATTTGAT 
TCGTTCAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTTCAGTA CTTCTACCCG 
GAGGAACTGG ATCAGCTCCT TTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GTAGGCCTGA TCATGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTTTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TATTTCTCCA GTTTGTGACT 
GGTAGCCCAA GATTGCCTGT TGGAGGATTC CGGAGTTTGA ATCCACCTTT GACAATTGTC 
CGAAAGACGT TTGAATCAAC AGAAAACCCA GATGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCATTGAGA TAATGCGTGA AAAACTGTTG 
ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335260.1
CDS	180..6161
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348331.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348331.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAATT AAAATGCCAG TTACAAAGGC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCTCTGG CTTTGGTACA AGCCATCGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGCGATGAC 
GATGGATCAG ATGAGGAAAT AGATGAGTCT CTGGCTGCTC AGTTCCTAAA TTCAGGAAAT 
GTAAGACACA GGCTGCAGTT TTATATTGGA GAACATTTGC TGCCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT TAGTATACAG GCTGAAGATG AAAGAGAATC CACAGATGAT 
GAGAGCAATC CTCTAGGCAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTATAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACAGCTC CAACGAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAC 
GATGGAGTGT GCCCATCAGT ATCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATAA CATTTGAAGA CCCGTCATTA GATGTGATCC TTCTTTTAAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA AATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTAACA GCAAAAGCAA ATAGGCAACT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTTACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGGCAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACCGAGA GGAGCTGCTG 
AAACAGGCGG AGTCTGTGAT GCAGGACCTC GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC GCTTGTATCT 
CAGGAACTAC AGAGAGCTGA CTTGGGTCTT TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGGA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCAAA GCCAGCTCAT ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGC 
TTACCCTTTT ATAAATGGAT GCTACGGCAA GAAACTTCAC TGACATCACA CGATTTGTTT 
GACATCGACC CAGTTGTAGC CAGATCAGTT TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TACAGTATGC ATTAGAAACC 
TTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGACTGG ATTTCACTCT GCCAGGGTTT 
CCCAATATCG AACTGAAGAA AGGAGGGAAG GATATACCAG TCACTATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCTAG GCAATTTGAT 
TCGTTCAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTTCAGTA CTTCTACCCG 
GAGGAACTGG ATCAGCTCCT TTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GTAGGCCTGA TCATGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTTTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TATTTCTCCA GTTTGTGACT 
GGTAGCCCAA GATTGCCTGT TGGAGGATTC CGGAGTTTGA ATCCACCTTT GACAATTGTC 
CGAAAGACGT TTGAATCAAC AGAAAACCCA GATGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCATTGAGA TAATGCGTGA AAAACTGTTG 
ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005291.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860780.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X32, mRNA.

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CloneID	OHu92734
Clone ID Related Accession (Same CDS sequence)	XM_017005291.1
Accession Version	XM_017005291.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC GAAGAAACGC 
AGTGAGTCTC CACCTGCTGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGGCAGT AAATTCTTCA 
GCTGCTCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCAGATGATT CCGAGATGGG ACGTTTGCAA 
GCTTTGTTAG AGGCAAGGGG TCTTCCCCCT CACCTATTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGT GGAGCTAGTT CTAAGGCCCA GCAGCTACTA 
CAAGGATTGC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CAGTTATTGA GATGTGTCAG 
TTACTGGTCA TGGGAAATGA GGAGACACTG GGAGGGTTTC CTGTCAAGAG TGTTGTTCCA 
GCTTTGATTA CGTTACTTCA GATGGAGCAC AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCTTCTG CTGTTGTAGT AGATGCTATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGAGACAT AGTAAAGCCA TTCTACAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTACCT AGAATTCTTC AGCATAAATG CCCAAAGAAA TGCATTAGCA 
ATTGCAGCTA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCAGATTCA 
CTCCCATTGC TAACCCAAAG GCTAACACAT CAGGATAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGCCTAGT GGACAACTTC CAGCATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTTAC AAATGTTCAA CAGCTGTTGG TAGTGACTCC ACCCATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACTTTAGCTG TTCAACTTAT GAAACAAAAC ATTGCAGAAA CGCTTCACTT TCTCCTGTGT 
GGTGCCTCCA ATGGAAGTTG TCAGGAACAG ATTGATCTTG TTCCACGAAG CCCTCAAGAG 
TTGTATGAAC TGACATCTCT GATTTGTGAA CTTATGCCAT GTTTACCAAA AGAAGGCATT 
TTTGCAGTTG ATACCATGTT GAAGAAGGGA AATGCACAGA ACACAGATGG TGCGATATGG 
CAGTGGCGTG ATGATCGGGG CCTCTGGCAT CCATATAACA GGATTGACAG CCGGATCATT 
GAGGTAGCAG CCCATCAGGT CGGTGAGGAT GAGATAAGCT TGTCCACTCT GGGACGAGTT 
TATACTATTG ATTTTAATTC TATGCAGCAA ATCAATGAGG ACACGGGAAC AGCACGTGCC 
ATTCAGAGAA AACCTAACCC GTTAGCCAAT AGTAACACTA GTGGATATTC AGAGTCAAAG 
AAGGATGATG CTCGAGCACA GCTTATGAAA GAGGATCCGG AACTGGCTAA GTCTTTTATT 
AAGACATTAT TTGGTGTTCT TTATGAAGTG TATAGTTCCT CAGCAGGACC TGCGGTCAGA 
CATAAGTGCC TTAGAGCAAT TCTTAGGATA ATTTATTTTG CGGATGCTGA ACTTCTGAAG 
GATGTTCTGA AAAATCATGC TGTTTCAAGT CACATTGCTT CCATGCTGTC AAGCCAAGAC 
CTGAAGATAG TAGTGGGAGC ACTTCAGATG GCAGAAATTT TAATGCAGAA GTTACCTGAT 
ATTTTTAGTG TTTACTTCAG AAGAGAAGGT GTAATGCATC AAGTAAAACA CTTAGCAGAA 
TCAGAGTCTT TGTTGACAAG TCCACCAAAG GCATGTACGA ATGGATCGGG ATCCATGGGA 
TCCACAACTT CAGTCAGCAG TGGGACAGCC ACAGCTGCCA CTCATGCTGC AGCTGACTTG 
GGATCACCCA GCTTGCAGCA CAGCAGGGAT GATTCTTTAG ATCTCAGCCC TCAAGGTCGA 
TTAAGTGATG TTCTAAAGAG AAAACGACTG CCAAAACGAG GGCCAAGAAG GCCAAAGTAC 
TCACCTCCAA GAGATGATGA CAAAGTAGAC AATCAAGCTA AAAGCCCCAC CACTACTCAG 
TCACCTAAAT CTTCTTTCCT GGCAAGCTTG AATCCAAAAA CATGGGGAAG GTTAAGTACA 
CAGTCCAACA GCAACAACAT TGAGCCAGCA CGGACTGCGG GAGGTAGTGG CCTTGCCAGG 
GCTGCCTCAA AGGATACCAT CTCCAATAAT AGAGAAAAAA TTAAAGGTTG GATTAAGGAG 
CAGGCACATA AATTTGTAGA ACGTTATTTC AGTTCTGAGA ATATGGATGG AAGCAACCCT 
GCATTGAATG TCCTTCAGAG ACTTTGTGCT GCAACCGAAC AACTCAACCT CCAGGTGGAT 
GGTGGAGCTG AGTGCCTTGT AGAAATCCGT AGCATAGTCT CAGAGTCAGA TGTTTCATCA 
TTTGAAATCC AACATAGTGG ATTTGTGAAG CAGCTGTTGC TTTATTTGAC ATCTAAAAGT 
GAAAAGGATG CTGTGAGCAG AGAGATCAGA TTAAAGCGAT TTCTTCATGT ATTTTTTTCT 
TCTCCACTTC CTGGAGAAGA GCCCATTGGA AGAGTGGAAC CAGTGGGTAA TGCACCTTTG 
TTGGCATTAG TTCACAAGAT GAACAACTGC CTCAGCCAGA TGGAACAATT TCCAGTCAAA 
GTACATGATT TCCCTAGTGG AAATGGGACA GGAGGCAGCT TTTCTCTCAA CAGAGGATCA 
CAGGCTTTAA AATTTTTCAA CACACATCAA TTAAAATGCC AGTTACAAAG GCATCCAGAC 
TGTGCAAATG TGAAGCAGTG GAAGGGTGGA CCTGTCAAGA TTGACCCTCT GGCTTTGGTA 
CAAGCCATCG AGAGATACCT TGTAGTTAGA GGGTATGGAA GAGTAAGAGA AGATGATGAA 
GACAGCGATG ACGATGGATC AGATGAGGAA ATAGATGAGT CTCTGGCTGC TCAGTTCCTA 
AATTCAGGAA ATGTAAGACA CAGGCTGCAG TTTTATATTG GAGAACATTT GCTGCCGTAT 
AACATGACTG TGTATCAGGC AGTACGGCAG TTTAGTATAC AGGCTGAAGA TGAAAGAGAA 
TCCACAGATG ATGAGAGCAA TCCTCTAGGC AGAGCTGGTA TTTGGACAAA GACTCATACA 
ATATGGTATA AACCTGTGAG AGAGGATGAA GAAAGTAATA AAGATTGTGT TGGTGGTAAA 
AGAGGAAGAG CCCAAACAGC TCCAACGAAA ACTTCCCCTA GAAATGCAAA AAAGCATGAT 
GAGTTATGGC ACGATGGAGT GTGCCCATCA GTATCAAATC CTTTAGAAGT TTACCTCATT 
CCCACACCAC CTGAAAATAT AACATTTGAA GACCCGTCAT TAGATGTGAT CCTTCTTTTA 
AGAGTTTTAC ATGCTATCAG TCGATACTGG TATTACTTGT ATGATAATGC AATGTGCAAG 
GAAATTATTC CAACTAGTGA ATTTATTAAC AGTAAGTTAA CAGCAAAAGC AAATAGGCAA 
CTTCAAGATC CTTTAGTAAT CATGACAGGA AACATCCCAA CATGGCTTAC TGAGCTAGGA 
AAAACCTGCC CATTTTTCTT TCCTTTTGAT ACCCGGCAAA TGCTTTTTTA TGTAACTGCA 
TTTGATCGGG ACCGAGCAAT GCAAAGATTA CTTGATACCA ACCCAGAAAT CAACCAGTCT 
GATTCTCAAG ATAGCAGAGT TGCACCTAGA TTGGATAGAA AAAAACGTAC TGTGAACCGA 
GAGGAGCTGC TGAAACAGGC GGAGTCTGTG ATGCAGGACC TCGGCAGCTC ACGGGCCATG 
TTAGAAATCC AGTATGAAAA TGAGGTTGGT ACAGGTCTTG GGCCTACACT GGAGTTTTAT 
GCGCTTGTAT CTCAGGAACT ACAGAGAGCT GACTTGGGTC TTTGGAGAGG TGAAGAAGTA 
ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA GGGACCAAGT ATATTCAAAA CCTCCAGGGC 
CTGTTTGCGC TTCCCTTTGG TAGGACAGCA AAGCCAGCTC ATATCGCAAA GGTTAAGATG 
AAGTTTCGCT TCTTAGGAAA ATTAATGGCC AAGGCTATCA TGGATTTCAG ATTGGTGGAC 
CTTCCCCTTG GCTTACCCTT TTATAAATGG ATGCTACGGC AAGAAACTTC ACTGACATCA 
CACGATTTGT TTGACATCGA CCCAGTTGTA GCCAGATCAG TTTATCACCT AGAAGACATT 
GTCAGACAGA AGAAAAGACT TGAACAAGAT AAATCCCAGA CCAAAGAGAG TCTACAGTAT 
GCATTAGAAA CCTTGACTAT GAATGGCTGC TCAGTTGAAG ATCTAGGACT GGATTTCACT 
CTGCCAGGGT TTCCCAATAT CGAACTGAAG AAAGGAGGGA AGGATATACC AGTCACTATC 
CACAATTTAG AGGAGTATCT AAGACTGGTT ATATTCTGGG CACTAAATGA AGGCGTTTCT 
AGGCAATTTG ATTCGTTCAG AGATGGATTT GAATCAGTCT TCCCACTCAG TCATCTTCAG 
TACTTCTACC CGGAGGAACT GGATCAGCTC CTTTGTGGCA GTAAAGCAGA CACTTGGGAT 
GCAAAGACAC TGATGGAATG CTGTAGGCCT GATCATGGTT ATACTCATGA CAGTCGGGCT 
GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT TTTGATAATG AGCAGCAGAG GTTATTTCTC 
CAGTTTGTGA CTGGTAGCCC AAGATTGCCT GTTGGAGGAT TCCGGAGTTT GAATCCACCT 
TTGACAATTG TCCGAAAGAC GTTTGAATCA ACAGAAAACC CAGATGACTT CTTGCCCTCT 
GTAATGACTT GTGTGAACTA TCTTAAGTTG CCGGACTATT CAAGCATTGA GATAATGCGT 
GAAAAACTGT TGATAGCAGC AAGAGAAGGG CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860780.1
CDS	180..6173
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005291.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X32, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005291.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC GAAGAAACGC 
AGTGAGTCTC CACCTGCTGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGGCAGT AAATTCTTCA 
GCTGCTCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCAGATGATT CCGAGATGGG ACGTTTGCAA 
GCTTTGTTAG AGGCAAGGGG TCTTCCCCCT CACCTATTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGT GGAGCTAGTT CTAAGGCCCA GCAGCTACTA 
CAAGGATTGC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CAGTTATTGA GATGTGTCAG 
TTACTGGTCA TGGGAAATGA GGAGACACTG GGAGGGTTTC CTGTCAAGAG TGTTGTTCCA 
GCTTTGATTA CGTTACTTCA GATGGAGCAC AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCTTCTG CTGTTGTAGT AGATGCTATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGAGACAT AGTAAAGCCA TTCTACAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTACCT AGAATTCTTC AGCATAAATG CCCAAAGAAA TGCATTAGCA 
ATTGCAGCTA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCAGATTCA 
CTCCCATTGC TAACCCAAAG GCTAACACAT CAGGATAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGCCTAGT GGACAACTTC CAGCATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTTAC AAATGTTCAA CAGCTGTTGG TAGTGACTCC ACCCATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACTTTAGCTG TTCAACTTAT GAAACAAAAC ATTGCAGAAA CGCTTCACTT TCTCCTGTGT 
GGTGCCTCCA ATGGAAGTTG TCAGGAACAG ATTGATCTTG TTCCACGAAG CCCTCAAGAG 
TTGTATGAAC TGACATCTCT GATTTGTGAA CTTATGCCAT GTTTACCAAA AGAAGGCATT 
TTTGCAGTTG ATACCATGTT GAAGAAGGGA AATGCACAGA ACACAGATGG TGCGATATGG 
CAGTGGCGTG ATGATCGGGG CCTCTGGCAT CCATATAACA GGATTGACAG CCGGATCATT 
GAGGTAGCAG CCCATCAGGT CGGTGAGGAT GAGATAAGCT TGTCCACTCT GGGACGAGTT 
TATACTATTG ATTTTAATTC TATGCAGCAA ATCAATGAGG ACACGGGAAC AGCACGTGCC 
ATTCAGAGAA AACCTAACCC GTTAGCCAAT AGTAACACTA GTGGATATTC AGAGTCAAAG 
AAGGATGATG CTCGAGCACA GCTTATGAAA GAGGATCCGG AACTGGCTAA GTCTTTTATT 
AAGACATTAT TTGGTGTTCT TTATGAAGTG TATAGTTCCT CAGCAGGACC TGCGGTCAGA 
CATAAGTGCC TTAGAGCAAT TCTTAGGATA ATTTATTTTG CGGATGCTGA ACTTCTGAAG 
GATGTTCTGA AAAATCATGC TGTTTCAAGT CACATTGCTT CCATGCTGTC AAGCCAAGAC 
CTGAAGATAG TAGTGGGAGC ACTTCAGATG GCAGAAATTT TAATGCAGAA GTTACCTGAT 
ATTTTTAGTG TTTACTTCAG AAGAGAAGGT GTAATGCATC AAGTAAAACA CTTAGCAGAA 
TCAGAGTCTT TGTTGACAAG TCCACCAAAG GCATGTACGA ATGGATCGGG ATCCATGGGA 
TCCACAACTT CAGTCAGCAG TGGGACAGCC ACAGCTGCCA CTCATGCTGC AGCTGACTTG 
GGATCACCCA GCTTGCAGCA CAGCAGGGAT GATTCTTTAG ATCTCAGCCC TCAAGGTCGA 
TTAAGTGATG TTCTAAAGAG AAAACGACTG CCAAAACGAG GGCCAAGAAG GCCAAAGTAC 
TCACCTCCAA GAGATGATGA CAAAGTAGAC AATCAAGCTA AAAGCCCCAC CACTACTCAG 
TCACCTAAAT CTTCTTTCCT GGCAAGCTTG AATCCAAAAA CATGGGGAAG GTTAAGTACA 
CAGTCCAACA GCAACAACAT TGAGCCAGCA CGGACTGCGG GAGGTAGTGG CCTTGCCAGG 
GCTGCCTCAA AGGATACCAT CTCCAATAAT AGAGAAAAAA TTAAAGGTTG GATTAAGGAG 
CAGGCACATA AATTTGTAGA ACGTTATTTC AGTTCTGAGA ATATGGATGG AAGCAACCCT 
GCATTGAATG TCCTTCAGAG ACTTTGTGCT GCAACCGAAC AACTCAACCT CCAGGTGGAT 
GGTGGAGCTG AGTGCCTTGT AGAAATCCGT AGCATAGTCT CAGAGTCAGA TGTTTCATCA 
TTTGAAATCC AACATAGTGG ATTTGTGAAG CAGCTGTTGC TTTATTTGAC ATCTAAAAGT 
GAAAAGGATG CTGTGAGCAG AGAGATCAGA TTAAAGCGAT TTCTTCATGT ATTTTTTTCT 
TCTCCACTTC CTGGAGAAGA GCCCATTGGA AGAGTGGAAC CAGTGGGTAA TGCACCTTTG 
TTGGCATTAG TTCACAAGAT GAACAACTGC CTCAGCCAGA TGGAACAATT TCCAGTCAAA 
GTACATGATT TCCCTAGTGG AAATGGGACA GGAGGCAGCT TTTCTCTCAA CAGAGGATCA 
CAGGCTTTAA AATTTTTCAA CACACATCAA TTAAAATGCC AGTTACAAAG GCATCCAGAC 
TGTGCAAATG TGAAGCAGTG GAAGGGTGGA CCTGTCAAGA TTGACCCTCT GGCTTTGGTA 
CAAGCCATCG AGAGATACCT TGTAGTTAGA GGGTATGGAA GAGTAAGAGA AGATGATGAA 
GACAGCGATG ACGATGGATC AGATGAGGAA ATAGATGAGT CTCTGGCTGC TCAGTTCCTA 
AATTCAGGAA ATGTAAGACA CAGGCTGCAG TTTTATATTG GAGAACATTT GCTGCCGTAT 
AACATGACTG TGTATCAGGC AGTACGGCAG TTTAGTATAC AGGCTGAAGA TGAAAGAGAA 
TCCACAGATG ATGAGAGCAA TCCTCTAGGC AGAGCTGGTA TTTGGACAAA GACTCATACA 
ATATGGTATA AACCTGTGAG AGAGGATGAA GAAAGTAATA AAGATTGTGT TGGTGGTAAA 
AGAGGAAGAG CCCAAACAGC TCCAACGAAA ACTTCCCCTA GAAATGCAAA AAAGCATGAT 
GAGTTATGGC ACGATGGAGT GTGCCCATCA GTATCAAATC CTTTAGAAGT TTACCTCATT 
CCCACACCAC CTGAAAATAT AACATTTGAA GACCCGTCAT TAGATGTGAT CCTTCTTTTA 
AGAGTTTTAC ATGCTATCAG TCGATACTGG TATTACTTGT ATGATAATGC AATGTGCAAG 
GAAATTATTC CAACTAGTGA ATTTATTAAC AGTAAGTTAA CAGCAAAAGC AAATAGGCAA 
CTTCAAGATC CTTTAGTAAT CATGACAGGA AACATCCCAA CATGGCTTAC TGAGCTAGGA 
AAAACCTGCC CATTTTTCTT TCCTTTTGAT ACCCGGCAAA TGCTTTTTTA TGTAACTGCA 
TTTGATCGGG ACCGAGCAAT GCAAAGATTA CTTGATACCA ACCCAGAAAT CAACCAGTCT 
GATTCTCAAG ATAGCAGAGT TGCACCTAGA TTGGATAGAA AAAAACGTAC TGTGAACCGA 
GAGGAGCTGC TGAAACAGGC GGAGTCTGTG ATGCAGGACC TCGGCAGCTC ACGGGCCATG 
TTAGAAATCC AGTATGAAAA TGAGGTTGGT ACAGGTCTTG GGCCTACACT GGAGTTTTAT 
GCGCTTGTAT CTCAGGAACT ACAGAGAGCT GACTTGGGTC TTTGGAGAGG TGAAGAAGTA 
ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA GGGACCAAGT ATATTCAAAA CCTCCAGGGC 
CTGTTTGCGC TTCCCTTTGG TAGGACAGCA AAGCCAGCTC ATATCGCAAA GGTTAAGATG 
AAGTTTCGCT TCTTAGGAAA ATTAATGGCC AAGGCTATCA TGGATTTCAG ATTGGTGGAC 
CTTCCCCTTG GCTTACCCTT TTATAAATGG ATGCTACGGC AAGAAACTTC ACTGACATCA 
CACGATTTGT TTGACATCGA CCCAGTTGTA GCCAGATCAG TTTATCACCT AGAAGACATT 
GTCAGACAGA AGAAAAGACT TGAACAAGAT AAATCCCAGA CCAAAGAGAG TCTACAGTAT 
GCATTAGAAA CCTTGACTAT GAATGGCTGC TCAGTTGAAG ATCTAGGACT GGATTTCACT 
CTGCCAGGGT TTCCCAATAT CGAACTGAAG AAAGGAGGGA AGGATATACC AGTCACTATC 
CACAATTTAG AGGAGTATCT AAGACTGGTT ATATTCTGGG CACTAAATGA AGGCGTTTCT 
AGGCAATTTG ATTCGTTCAG AGATGGATTT GAATCAGTCT TCCCACTCAG TCATCTTCAG 
TACTTCTACC CGGAGGAACT GGATCAGCTC CTTTGTGGCA GTAAAGCAGA CACTTGGGAT 
GCAAAGACAC TGATGGAATG CTGTAGGCCT GATCATGGTT ATACTCATGA CAGTCGGGCT 
GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT TTTGATAATG AGCAGCAGAG GTTATTTCTC 
CAGTTTGTGA CTGGTAGCCC AAGATTGCCT GTTGGAGGAT TCCGGAGTTT GAATCCACCT 
TTGACAATTG TCCGAAAGAC GTTTGAATCA ACAGAAAACC CAGATGACTT CTTGCCCTCT 
GTAATGACTT GTGTGAACTA TCTTAAGTTG CCGGACTATT CAAGCATTGA GATAATGCGT 
GAAAAACTGT TGATAGCAGC AAGAGAAGGG CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001284216.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271145.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4, mRNA.

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CloneID	OHu07833
Clone ID Related Accession (Same CDS sequence)	NM_001284216.1 , NM_001284216.2
Accession Version	NM_001284216.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240964.1, D28476.1, BC113891.1, AC093384.4 and BM677880.1. On Sep 26, 2013 this sequence version replaced XM_005246964.1. Transcript Variant: This variant (4) encodes isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC113891.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGTC GGCGAGGCTC TGGCCTGGGC 
AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAAATGG CAGACCCTGA AAGCAACCAG 
GAGGCAGTAA ATTCTTCAGC TGCTCGGACA GATGAAGCTC CCCAAGGAGC TGCAGCCTCT 
AGTTCTGTTG CAGGGGCTGT TGGCATGACC ACCTCTGGGG AGAGTGAATC AGATGATTCC 
GAGATGGGAC GTTTGCAAGC TTTGTTAGAG GCAAGGGGTC TTCCCCCTCA CCTATTTGGT 
CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 
AAGGCCCAGC AGCTACTACA AGGATTGCAA GCCAGTGATG AAAGTCAACA GCTTCAGGCA 
GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGAAATGAGG AGACACTGGG AGGGTTTCCT 
GTCAAGAGTG TTGTTCCAGC TTTGATTACG TTACTTCAGA TGGAGCACAA TTTTGATATT 
ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCTTCTGCT 
GTTGTAGTAG ATGCTATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 
GTGGCAGAGC AGGCCTTGAC TGCCTTGGAG ATGTTGTCAC GGAGACATAG TAAAGCCATT 
CTACAGGCGG GTGGTTTGGC AGACTGCTTG CTGTACCTAG AATTCTTCAG CATAAATGCC 
CAAAGAAATG CATTAGCAAT TGCAGCTAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 
CATTTTGTGG CAGATTCACT CCCATTGCTA ACCCAAAGGC TAACACATCA GGATAAAAAG 
TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGCCTAGTGG ACAACTTCCA GCATGAGGAG 
AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTTACAA ATGTTCAACA GCTGTTGGTA 
GTGACTCCAC CCATTTTAAG TTCTGGGATG TTTATAATGG TGGTTCGCAT GTTTTCTCTG 
ATGTGTTCCA ACTGTCCAAC TTTAGCTGTT CAACTTATGA AACAAAACAT TGCAGAAACG 
CTTCACTTTC TCCTGTGTGG TGCCTCCAAT GGAAGTTGTC AGGAACAGAT TGATCTTGTT 
CCACGAAGCC CTCAAGAGTT GTATGAACTG ACATCTCTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATTTT TGCAGTTGAT ACCATGTTGA AGAAGGGAAA TGCACAGAAC 
ACAGATGGTG CGATATGGCA GTGGCGTGAT GATCGGGGCC TCTGGCATCC ATATAACAGG 
ATTGACAGCC GGATCATTGA GGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCGTTAG CCAATAGTAA CACTAGTGGA 
TATTCAGAGT CAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCGGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTG GGAGCACTTC AGATGGCAGA AATTTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCAC CAAAGGCATG TACGAATGGA 
TCGGGATCCA TGGGATCCAC AACTTCAGTC AGCAGTGGGA CAGCCACAGC TGCCACTCAT 
GCTGCAGCTG ACTTGGGATC ACCCAGCTTG CAGCACAGCA GGGATGATTC TTTAGATCTC 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AGTACTCACC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCT TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GTACACAGTC CAACAGCAAC AACATTGAGC CAGCACGGAC TGCGGGAGGT 
AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AGGAGCAGGC ACATAAATTT GTAGAACGTT ATTTCAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCAAC CGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CATCATTTGA AATCCAACAT AGTGGATTTG TGAAGCAGCT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GCGATTTCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGGAAGAGT GGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAGATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGAAATG GGACAGGAGG CAGCTTTTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271145.1
CDS	180..5348
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001284216.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284216.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGTC GGCGAGGCTC TGGCCTGGGC 
AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAAATGG CAGACCCTGA AAGCAACCAG 
GAGGCAGTAA ATTCTTCAGC TGCTCGGACA GATGAAGCTC CCCAAGGAGC TGCAGCCTCT 
AGTTCTGTTG CAGGGGCTGT TGGCATGACC ACCTCTGGGG AGAGTGAATC AGATGATTCC 
GAGATGGGAC GTTTGCAAGC TTTGTTAGAG GCAAGGGGTC TTCCCCCTCA CCTATTTGGT 
CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 
AAGGCCCAGC AGCTACTACA AGGATTGCAA GCCAGTGATG AAAGTCAACA GCTTCAGGCA 
GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGAAATGAGG AGACACTGGG AGGGTTTCCT 
GTCAAGAGTG TTGTTCCAGC TTTGATTACG TTACTTCAGA TGGAGCACAA TTTTGATATT 
ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCTTCTGCT 
GTTGTAGTAG ATGCTATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 
GTGGCAGAGC AGGCCTTGAC TGCCTTGGAG ATGTTGTCAC GGAGACATAG TAAAGCCATT 
CTACAGGCGG GTGGTTTGGC AGACTGCTTG CTGTACCTAG AATTCTTCAG CATAAATGCC 
CAAAGAAATG CATTAGCAAT TGCAGCTAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 
CATTTTGTGG CAGATTCACT CCCATTGCTA ACCCAAAGGC TAACACATCA GGATAAAAAG 
TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGCCTAGTGG ACAACTTCCA GCATGAGGAG 
AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTTACAA ATGTTCAACA GCTGTTGGTA 
GTGACTCCAC CCATTTTAAG TTCTGGGATG TTTATAATGG TGGTTCGCAT GTTTTCTCTG 
ATGTGTTCCA ACTGTCCAAC TTTAGCTGTT CAACTTATGA AACAAAACAT TGCAGAAACG 
CTTCACTTTC TCCTGTGTGG TGCCTCCAAT GGAAGTTGTC AGGAACAGAT TGATCTTGTT 
CCACGAAGCC CTCAAGAGTT GTATGAACTG ACATCTCTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATTTT TGCAGTTGAT ACCATGTTGA AGAAGGGAAA TGCACAGAAC 
ACAGATGGTG CGATATGGCA GTGGCGTGAT GATCGGGGCC TCTGGCATCC ATATAACAGG 
ATTGACAGCC GGATCATTGA GGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCGTTAG CCAATAGTAA CACTAGTGGA 
TATTCAGAGT CAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCGGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTG GGAGCACTTC AGATGGCAGA AATTTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCAC CAAAGGCATG TACGAATGGA 
TCGGGATCCA TGGGATCCAC AACTTCAGTC AGCAGTGGGA CAGCCACAGC TGCCACTCAT 
GCTGCAGCTG ACTTGGGATC ACCCAGCTTG CAGCACAGCA GGGATGATTC TTTAGATCTC 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AGTACTCACC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCT TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GTACACAGTC CAACAGCAAC AACATTGAGC CAGCACGGAC TGCGGGAGGT 
AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AGGAGCAGGC ACATAAATTT GTAGAACGTT ATTTCAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCAAC CGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CATCATTTGA AATCCAACAT AGTGGATTTG TGAAGCAGCT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GCGATTTCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGGAAGAGT GGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAGATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGAAATG GGACAGGAGG CAGCTTTTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001284216.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271145.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu07833
Clone ID Related Accession (Same CDS sequence)	NM_001284216.1 , NM_001284216.2
Accession Version	NM_001284216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240964.1, D28476.1, BC113891.1, AC093384.4 and BM677880.1. On Jun 2, 2019 this sequence version replaced NM_001284216.1. Transcript Variant: This variant (4) encodes isoform d. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC113891.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGTC GGCGAGGCTC TGGCCTGGGC 
AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAAATGG CAGACCCTGA AAGCAACCAG 
GAGGCAGTAA ATTCTTCAGC TGCTCGGACA GATGAAGCTC CCCAAGGAGC TGCAGCCTCT 
AGTTCTGTTG CAGGGGCTGT TGGCATGACC ACCTCTGGGG AGAGTGAATC AGATGATTCC 
GAGATGGGAC GTTTGCAAGC TTTGTTAGAG GCAAGGGGTC TTCCCCCTCA CCTATTTGGT 
CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 
AAGGCCCAGC AGCTACTACA AGGATTGCAA GCCAGTGATG AAAGTCAACA GCTTCAGGCA 
GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGAAATGAGG AGACACTGGG AGGGTTTCCT 
GTCAAGAGTG TTGTTCCAGC TTTGATTACG TTACTTCAGA TGGAGCACAA TTTTGATATT 
ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCTTCTGCT 
GTTGTAGTAG ATGCTATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 
GTGGCAGAGC AGGCCTTGAC TGCCTTGGAG ATGTTGTCAC GGAGACATAG TAAAGCCATT 
CTACAGGCGG GTGGTTTGGC AGACTGCTTG CTGTACCTAG AATTCTTCAG CATAAATGCC 
CAAAGAAATG CATTAGCAAT TGCAGCTAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 
CATTTTGTGG CAGATTCACT CCCATTGCTA ACCCAAAGGC TAACACATCA GGATAAAAAG 
TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGCCTAGTGG ACAACTTCCA GCATGAGGAG 
AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTTACAA ATGTTCAACA GCTGTTGGTA 
GTGACTCCAC CCATTTTAAG TTCTGGGATG TTTATAATGG TGGTTCGCAT GTTTTCTCTG 
ATGTGTTCCA ACTGTCCAAC TTTAGCTGTT CAACTTATGA AACAAAACAT TGCAGAAACG 
CTTCACTTTC TCCTGTGTGG TGCCTCCAAT GGAAGTTGTC AGGAACAGAT TGATCTTGTT 
CCACGAAGCC CTCAAGAGTT GTATGAACTG ACATCTCTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATTTT TGCAGTTGAT ACCATGTTGA AGAAGGGAAA TGCACAGAAC 
ACAGATGGTG CGATATGGCA GTGGCGTGAT GATCGGGGCC TCTGGCATCC ATATAACAGG 
ATTGACAGCC GGATCATTGA GGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCGTTAG CCAATAGTAA CACTAGTGGA 
TATTCAGAGT CAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCGGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTG GGAGCACTTC AGATGGCAGA AATTTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCAC CAAAGGCATG TACGAATGGA 
TCGGGATCCA TGGGATCCAC AACTTCAGTC AGCAGTGGGA CAGCCACAGC TGCCACTCAT 
GCTGCAGCTG ACTTGGGATC ACCCAGCTTG CAGCACAGCA GGGATGATTC TTTAGATCTC 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AGTACTCACC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCT TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GTACACAGTC CAACAGCAAC AACATTGAGC CAGCACGGAC TGCGGGAGGT 
AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AGGAGCAGGC ACATAAATTT GTAGAACGTT ATTTCAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCAAC CGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CATCATTTGA AATCCAACAT AGTGGATTTG TGAAGCAGCT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GCGATTTCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGGAAGAGT GGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAGATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGAAATG GGACAGGAGG CAGCTTTTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271145.1
CDS	180..5348
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001284216.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284216.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGTC GGCGAGGCTC TGGCCTGGGC 
AAAAGAGGAG CAGCTGAAGC TCGTCGACAG GAGAAAATGG CAGACCCTGA AAGCAACCAG 
GAGGCAGTAA ATTCTTCAGC TGCTCGGACA GATGAAGCTC CCCAAGGAGC TGCAGCCTCT 
AGTTCTGTTG CAGGGGCTGT TGGCATGACC ACCTCTGGGG AGAGTGAATC AGATGATTCC 
GAGATGGGAC GTTTGCAAGC TTTGTTAGAG GCAAGGGGTC TTCCCCCTCA CCTATTTGGT 
CCTCTTGGTC CTCGGATGTC ACAGCTTTTC CATAGAACAA TTGGAAGTGG AGCTAGTTCT 
AAGGCCCAGC AGCTACTACA AGGATTGCAA GCCAGTGATG AAAGTCAACA GCTTCAGGCA 
GTTATTGAGA TGTGTCAGTT ACTGGTCATG GGAAATGAGG AGACACTGGG AGGGTTTCCT 
GTCAAGAGTG TTGTTCCAGC TTTGATTACG TTACTTCAGA TGGAGCACAA TTTTGATATT 
ATGAACCATG CTTGTCGAGC CTTAACATAC ATGATGGAAG CACTTCCTCG ATCTTCTGCT 
GTTGTAGTAG ATGCTATTCC TGTCTTTTTA GAAAAGCTGC AAGTTATTCA GTGTATTGAT 
GTGGCAGAGC AGGCCTTGAC TGCCTTGGAG ATGTTGTCAC GGAGACATAG TAAAGCCATT 
CTACAGGCGG GTGGTTTGGC AGACTGCTTG CTGTACCTAG AATTCTTCAG CATAAATGCC 
CAAAGAAATG CATTAGCAAT TGCAGCTAAT TGCTGCCAGA GTATCACGCC AGATGAATTT 
CATTTTGTGG CAGATTCACT CCCATTGCTA ACCCAAAGGC TAACACATCA GGATAAAAAG 
TCAGTAGAAA GCACTTGCCT TTGTTTTGCA CGCCTAGTGG ACAACTTCCA GCATGAGGAG 
AATTTACTCC AGCAGGTTGC TTCCAAAGAT CTGCTTACAA ATGTTCAACA GCTGTTGGTA 
GTGACTCCAC CCATTTTAAG TTCTGGGATG TTTATAATGG TGGTTCGCAT GTTTTCTCTG 
ATGTGTTCCA ACTGTCCAAC TTTAGCTGTT CAACTTATGA AACAAAACAT TGCAGAAACG 
CTTCACTTTC TCCTGTGTGG TGCCTCCAAT GGAAGTTGTC AGGAACAGAT TGATCTTGTT 
CCACGAAGCC CTCAAGAGTT GTATGAACTG ACATCTCTGA TTTGTGAACT TATGCCATGT 
TTACCAAAAG AAGGCATTTT TGCAGTTGAT ACCATGTTGA AGAAGGGAAA TGCACAGAAC 
ACAGATGGTG CGATATGGCA GTGGCGTGAT GATCGGGGCC TCTGGCATCC ATATAACAGG 
ATTGACAGCC GGATCATTGA GGCAGCCCAT CAGGTCGGTG AGGATGAGAT AAGCTTGTCC 
ACTCTGGGAC GAGTTTATAC TATTGATTTT AATTCTATGC AGCAAATCAA TGAGGACACG 
GGAACAGCAC GTGCCATTCA GAGAAAACCT AACCCGTTAG CCAATAGTAA CACTAGTGGA 
TATTCAGAGT CAAAGAAGGA TGATGCTCGA GCACAGCTTA TGAAAGAGGA TCCGGAACTG 
GCTAAGTCTT TTATTAAGAC ATTATTTGGT GTTCTTTATG AAGTGTATAG TTCCTCAGCA 
GGACCTGCGG TCAGACATAA GTGCCTTAGA GCAATTCTTA GGATAATTTA TTTTGCGGAT 
GCTGAACTTC TGAAGGATGT TCTGAAAAAT CATGCTGTTT CAAGTCACAT TGCTTCCATG 
CTGTCAAGCC AAGACCTGAA GATAGTAGTG GGAGCACTTC AGATGGCAGA AATTTTAATG 
CAGAAGTTAC CTGATATTTT TAGTGTTTAC TTCAGAAGAG AAGGTGTAAT GCATCAAGTA 
AAACACTTAG CAGAATCAGA GTCTTTGTTG ACAAGTCCAC CAAAGGCATG TACGAATGGA 
TCGGGATCCA TGGGATCCAC AACTTCAGTC AGCAGTGGGA CAGCCACAGC TGCCACTCAT 
GCTGCAGCTG ACTTGGGATC ACCCAGCTTG CAGCACAGCA GGGATGATTC TTTAGATCTC 
AGCCCTCAAG GTCGATTAAG TGATGTTCTA AAGAGAAAAC GACTGCCAAA ACGAGGGCCA 
AGAAGGCCAA AGTACTCACC TCCAAGAGAT GATGACAAAG TAGACAATCA AGCTAAAAGC 
CCCACCACTA CTCAGTCACC TAAATCTTCT TTCCTGGCAA GCTTGAATCC AAAAACATGG 
GGAAGGTTAA GTACACAGTC CAACAGCAAC AACATTGAGC CAGCACGGAC TGCGGGAGGT 
AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT ACCATCTCCA ATAATAGAGA AAAAATTAAA 
GGTTGGATTA AGGAGCAGGC ACATAAATTT GTAGAACGTT ATTTCAGTTC TGAGAATATG 
GATGGAAGCA ACCCTGCATT GAATGTCCTT CAGAGACTTT GTGCTGCAAC CGAACAACTC 
AACCTCCAGG TGGATGGTGG AGCTGAGTGC CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG 
TCAGATGTTT CATCATTTGA AATCCAACAT AGTGGATTTG TGAAGCAGCT GTTGCTTTAT 
TTGACATCTA AAAGTGAAAA GGATGCTGTG AGCAGAGAGA TCAGATTAAA GCGATTTCTT 
CATGTATTTT TTTCTTCTCC ACTTCCTGGA GAAGAGCCCA TTGGAAGAGT GGAACCAGTG 
GGTAATGCAC CTTTGTTGGC ATTAGTTCAC AAGATGAACA ACTGCCTCAG CCAGATGGAA 
CAATTTCCAG TCAAAGTACA TGATTTCCCT AGTGGAAATG GGACAGGAGG CAGCTTTTCT 
CTCAACAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_004238.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004229.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 3, mRNA.

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CloneID	OHu08708
Clone ID Related Accession (Same CDS sequence)	NM_004238.3 , NM_004238.2
Accession Version	NM_004238.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240964.1, D28476.1, AC093384.4 and BM677880.1. On Jun 2, 2019 this sequence version replaced NM_004238.2. Transcript Variant: This variant (3) encodes isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D28476.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGGGGCTG TTGGCATGAC CACCTCTGGG GAGAGTGAAT CAGATGATTC CGAGATGGGA 
CGTTTGCAAG CTTTGTTAGA GGCAAGGGGT CTTCCCCCTC ACCTATTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGTG GAGCTAGTTC TAAGGCCCAG 
CAGCTACTAC AAGGATTGCA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC AGTTATTGAG 
ATGTGTCAGT TACTGGTCAT GGGAAATGAG GAGACACTGG GAGGGTTTCC TGTCAAGAGT 
GTTGTTCCAG CTTTGATTAC GTTACTTCAG ATGGAGCACA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCTTCTGC TGTTGTAGTA 
GATGCTATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGAGACATA GTAAAGCCAT TCTACAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTACCTA GAATTCTTCA GCATAAATGC CCAAAGAAAT 
GCATTAGCAA TTGCAGCTAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCAGATTCAC TCCCATTGCT AACCCAAAGG CTAACACATC AGGATAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGCCTAGTG GACAACTTCC AGCATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTTACA AATGTTCAAC AGCTGTTGGT AGTGACTCCA 
CCCATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CTTTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAACAGA TTGATCTTGT TCCACGAAGC 
CCTCAAGAGT TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATTT TTGCAGTTGA TACCATGTTG AAGAAGGGAA ATGCACAGAA CACAGATGGT 
GCGATATGGC AGTGGCGTGA TGATCGGGGC CTCTGGCATC CATATAACAG GATTGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004229.1
CDS	180..6158
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_004238.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004238.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGGGGCTG TTGGCATGAC CACCTCTGGG GAGAGTGAAT CAGATGATTC CGAGATGGGA 
CGTTTGCAAG CTTTGTTAGA GGCAAGGGGT CTTCCCCCTC ACCTATTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGTG GAGCTAGTTC TAAGGCCCAG 
CAGCTACTAC AAGGATTGCA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC AGTTATTGAG 
ATGTGTCAGT TACTGGTCAT GGGAAATGAG GAGACACTGG GAGGGTTTCC TGTCAAGAGT 
GTTGTTCCAG CTTTGATTAC GTTACTTCAG ATGGAGCACA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCTTCTGC TGTTGTAGTA 
GATGCTATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGAGACATA GTAAAGCCAT TCTACAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTACCTA GAATTCTTCA GCATAAATGC CCAAAGAAAT 
GCATTAGCAA TTGCAGCTAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCAGATTCAC TCCCATTGCT AACCCAAAGG CTAACACATC AGGATAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGCCTAGTG GACAACTTCC AGCATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTTACA AATGTTCAAC AGCTGTTGGT AGTGACTCCA 
CCCATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CTTTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAACAGA TTGATCTTGT TCCACGAAGC 
CCTCAAGAGT TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATTT TTGCAGTTGA TACCATGTTG AAGAAGGGAA ATGCACAGAA CACAGATGGT 
GCGATATGGC AGTGGCGTGA TGATCGGGGC CTCTGGCATC CATATAACAG GATTGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_004238.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004229.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 3, mRNA.

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CloneID	OHu08708
Clone ID Related Accession (Same CDS sequence)	NM_004238.3 , NM_004238.2
Accession Version	NM_004238.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240964.1, D28476.1, AC093384.4 and BM677880.1. On Sep 26, 2013 this sequence version replaced NM_004238.1. Transcript Variant: This variant (3) encodes isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D28476.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGGGGCTG TTGGCATGAC CACCTCTGGG GAGAGTGAAT CAGATGATTC CGAGATGGGA 
CGTTTGCAAG CTTTGTTAGA GGCAAGGGGT CTTCCCCCTC ACCTATTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGTG GAGCTAGTTC TAAGGCCCAG 
CAGCTACTAC AAGGATTGCA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC AGTTATTGAG 
ATGTGTCAGT TACTGGTCAT GGGAAATGAG GAGACACTGG GAGGGTTTCC TGTCAAGAGT 
GTTGTTCCAG CTTTGATTAC GTTACTTCAG ATGGAGCACA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCTTCTGC TGTTGTAGTA 
GATGCTATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGAGACATA GTAAAGCCAT TCTACAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTACCTA GAATTCTTCA GCATAAATGC CCAAAGAAAT 
GCATTAGCAA TTGCAGCTAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCAGATTCAC TCCCATTGCT AACCCAAAGG CTAACACATC AGGATAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGCCTAGTG GACAACTTCC AGCATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTTACA AATGTTCAAC AGCTGTTGGT AGTGACTCCA 
CCCATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CTTTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAACAGA TTGATCTTGT TCCACGAAGC 
CCTCAAGAGT TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATTT TTGCAGTTGA TACCATGTTG AAGAAGGGAA ATGCACAGAA CACAGATGGT 
GCGATATGGC AGTGGCGTGA TGATCGGGGC CTCTGGCATC CATATAACAG GATTGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004229.1
CDS	180..6158
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_004238.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004238.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGGGGCTG TTGGCATGAC CACCTCTGGG GAGAGTGAAT CAGATGATTC CGAGATGGGA 
CGTTTGCAAG CTTTGTTAGA GGCAAGGGGT CTTCCCCCTC ACCTATTTGG TCCTCTTGGT 
CCTCGGATGT CACAGCTTTT CCATAGAACA ATTGGAAGTG GAGCTAGTTC TAAGGCCCAG 
CAGCTACTAC AAGGATTGCA AGCCAGTGAT GAAAGTCAAC AGCTTCAGGC AGTTATTGAG 
ATGTGTCAGT TACTGGTCAT GGGAAATGAG GAGACACTGG GAGGGTTTCC TGTCAAGAGT 
GTTGTTCCAG CTTTGATTAC GTTACTTCAG ATGGAGCACA ATTTTGATAT TATGAACCAT 
GCTTGTCGAG CCTTAACATA CATGATGGAA GCACTTCCTC GATCTTCTGC TGTTGTAGTA 
GATGCTATTC CTGTCTTTTT AGAAAAGCTG CAAGTTATTC AGTGTATTGA TGTGGCAGAG 
CAGGCCTTGA CTGCCTTGGA GATGTTGTCA CGGAGACATA GTAAAGCCAT TCTACAGGCG 
GGTGGTTTGG CAGACTGCTT GCTGTACCTA GAATTCTTCA GCATAAATGC CCAAAGAAAT 
GCATTAGCAA TTGCAGCTAA TTGCTGCCAG AGTATCACGC CAGATGAATT TCATTTTGTG 
GCAGATTCAC TCCCATTGCT AACCCAAAGG CTAACACATC AGGATAAAAA GTCAGTAGAA 
AGCACTTGCC TTTGTTTTGC ACGCCTAGTG GACAACTTCC AGCATGAGGA GAATTTACTC 
CAGCAGGTTG CTTCCAAAGA TCTGCTTACA AATGTTCAAC AGCTGTTGGT AGTGACTCCA 
CCCATTTTAA GTTCTGGGAT GTTTATAATG GTGGTTCGCA TGTTTTCTCT GATGTGTTCC 
AACTGTCCAA CTTTAGCTGT TCAACTTATG AAACAAAACA TTGCAGAAAC GCTTCACTTT 
CTCCTGTGTG GTGCCTCCAA TGGAAGTTGT CAGGAACAGA TTGATCTTGT TCCACGAAGC 
CCTCAAGAGT TGTATGAACT GACATCTCTG ATTTGTGAAC TTATGCCATG TTTACCAAAA 
GAAGGCATTT TTGCAGTTGA TACCATGTTG AAGAAGGGAA ATGCACAGAA CACAGATGGT 
GCGATATGGC AGTGGCGTGA TGATCGGGGC CTCTGGCATC CATATAACAG GATTGACAGC 
CGGATCATTG AGCAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005293.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860782.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X34, mRNA.

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CloneID	OHu36810
Clone ID Related Accession (Same CDS sequence)	XM_017005293.1 , XM_024453243.1
Accession Version	XM_017005293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAATTAAAAT GCCAGTTACA AAGGCATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC TCTGGCTTTG 
GTACAAGCCA TCGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGCG ATGACGATGG ATCAGATGAG GAAATAGATG AGTCTCTGGC TGCTCAGTTC 
CTAAATTCAG GAAATGTAAG ACACAGGCTG CAGTTTTATA TTGGAGAACA TTTGCTGCCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTTAGTA TACAGGCTGA AGATGAAAGA 
GAATCCACAG ATGATGAGAG CAATCCTCTA GGCAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ATAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC AGCTCCAACG AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCACGATGG AGTGTGCCCA TCAGTATCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATAACATTT GAAGACCCGT CATTAGATGT GATCCTTCTT 
TTAAGAGTTT TACATGCTAT CAGTCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAAATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TAACAGCAAA AGCAAATAGG 
CAACTTCAAG ATCCTTTAGT AATCATGACA GGAAACATCC CAACATGGCT TACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGGC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
CGAGAGGAGC TGCTGAAACA GGCGGAGTCT GTGATGCAGG ACCTCGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCGCTTG TATCTCAGGA ACTACAGAGA GCTGACTTGG GTCTTTGGAG AGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGGAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCAAAGCCAG CTCATATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGCTTACC CTTTTATAAA TGGATGCTAC GGCAAGAAAC TTCACTGACA 
TCACACGATT TGTTTGACAT CGACCCAGTT GTAGCCAGAT CAGTTTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTACAG 
TATGCATTAG AAACCTTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ACTGGATTTC 
ACTCTGCCAG GGTTTCCCAA TATCGAACTG AAGAAAGGAG GGAAGGATAT ACCAGTCACT 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCTAGGCAAT TTGATTCGTT CAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTT 
CAGTACTTCT ACCCGGAGGA ACTGGATCAG CTCCTTTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGTAGG CCTGATCATG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TTTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTATTT 
CTCCAGTTTG TGACTGGTAG CCCAAGATTG CCTGTTGGAG GATTCCGGAG TTTGAATCCA 
CCTTTGACAA TTGTCCGAAA GACGTTTGAA TCAACAGAAA ACCCAGATGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCAT TGAGATAATG 
CGTGAAAAAC TGTTGATAGC AGCAAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860782.1
CDS	180..6176
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005293.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X34, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005293.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAATTAAAAT GCCAGTTACA AAGGCATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC TCTGGCTTTG 
GTACAAGCCA TCGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGCG ATGACGATGG ATCAGATGAG GAAATAGATG AGTCTCTGGC TGCTCAGTTC 
CTAAATTCAG GAAATGTAAG ACACAGGCTG CAGTTTTATA TTGGAGAACA TTTGCTGCCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTTAGTA TACAGGCTGA AGATGAAAGA 
GAATCCACAG ATGATGAGAG CAATCCTCTA GGCAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ATAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC AGCTCCAACG AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCACGATGG AGTGTGCCCA TCAGTATCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATAACATTT GAAGACCCGT CATTAGATGT GATCCTTCTT 
TTAAGAGTTT TACATGCTAT CAGTCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAAATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TAACAGCAAA AGCAAATAGG 
CAACTTCAAG ATCCTTTAGT AATCATGACA GGAAACATCC CAACATGGCT TACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGGC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
CGAGAGGAGC TGCTGAAACA GGCGGAGTCT GTGATGCAGG ACCTCGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCGCTTG TATCTCAGGA ACTACAGAGA GCTGACTTGG GTCTTTGGAG AGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGGAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCAAAGCCAG CTCATATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGCTTACC CTTTTATAAA TGGATGCTAC GGCAAGAAAC TTCACTGACA 
TCACACGATT TGTTTGACAT CGACCCAGTT GTAGCCAGAT CAGTTTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTACAG 
TATGCATTAG AAACCTTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ACTGGATTTC 
ACTCTGCCAG GGTTTCCCAA TATCGAACTG AAGAAAGGAG GGAAGGATAT ACCAGTCACT 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCTAGGCAAT TTGATTCGTT CAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTT 
CAGTACTTCT ACCCGGAGGA ACTGGATCAG CTCCTTTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGTAGG CCTGATCATG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TTTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTATTT 
CTCCAGTTTG TGACTGGTAG CCCAAGATTG CCTGTTGGAG GATTCCGGAG TTTGAATCCA 
CCTTTGACAA TTGTCCGAAA GACGTTTGAA TCAACAGAAA ACCCAGATGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCAT TGAGATAATG 
CGTGAAAAAC TGTTGATAGC AGCAAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453243.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309011.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.

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CloneID	OHu36810
Clone ID Related Accession (Same CDS sequence)	XM_017005293.1 , XM_024453243.1
Accession Version	XM_024453243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAATTAAAAT GCCAGTTACA AAGGCATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC TCTGGCTTTG 
GTACAAGCCA TCGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGCG ATGACGATGG ATCAGATGAG GAAATAGATG AGTCTCTGGC TGCTCAGTTC 
CTAAATTCAG GAAATGTAAG ACACAGGCTG CAGTTTTATA TTGGAGAACA TTTGCTGCCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTTAGTA TACAGGCTGA AGATGAAAGA 
GAATCCACAG ATGATGAGAG CAATCCTCTA GGCAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ATAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC AGCTCCAACG AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCACGATGG AGTGTGCCCA TCAGTATCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATAACATTT GAAGACCCGT CATTAGATGT GATCCTTCTT 
TTAAGAGTTT TACATGCTAT CAGTCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAAATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TAACAGCAAA AGCAAATAGG 
CAACTTCAAG ATCCTTTAGT AATCATGACA GGAAACATCC CAACATGGCT TACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGGC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
CGAGAGGAGC TGCTGAAACA GGCGGAGTCT GTGATGCAGG ACCTCGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCGCTTG TATCTCAGGA ACTACAGAGA GCTGACTTGG GTCTTTGGAG AGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGGAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCAAAGCCAG CTCATATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGCTTACC CTTTTATAAA TGGATGCTAC GGCAAGAAAC TTCACTGACA 
TCACACGATT TGTTTGACAT CGACCCAGTT GTAGCCAGAT CAGTTTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTACAG 
TATGCATTAG AAACCTTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ACTGGATTTC 
ACTCTGCCAG GGTTTCCCAA TATCGAACTG AAGAAAGGAG GGAAGGATAT ACCAGTCACT 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCTAGGCAAT TTGATTCGTT CAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTT 
CAGTACTTCT ACCCGGAGGA ACTGGATCAG CTCCTTTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGTAGG CCTGATCATG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TTTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTATTT 
CTCCAGTTTG TGACTGGTAG CCCAAGATTG CCTGTTGGAG GATTCCGGAG TTTGAATCCA 
CCTTTGACAA TTGTCCGAAA GACGTTTGAA TCAACAGAAA ACCCAGATGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCAT TGAGATAATG 
CGTGAAAAAC TGTTGATAGC AGCAAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309011.1
CDS	50..6046
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453243.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453243.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GCTTTTCTCT CAACAGAGGA 
TCACAGGCTT TAAAATTTTT CAACACACAT CAATTAAAAT GCCAGTTACA AAGGCATCCA 
GACTGTGCAA ATGTGAAGCA GTGGAAGGGT GGACCTGTCA AGATTGACCC TCTGGCTTTG 
GTACAAGCCA TCGAGAGATA CCTTGTAGTT AGAGGGTATG GAAGAGTAAG AGAAGATGAT 
GAAGACAGCG ATGACGATGG ATCAGATGAG GAAATAGATG AGTCTCTGGC TGCTCAGTTC 
CTAAATTCAG GAAATGTAAG ACACAGGCTG CAGTTTTATA TTGGAGAACA TTTGCTGCCG 
TATAACATGA CTGTGTATCA GGCAGTACGG CAGTTTAGTA TACAGGCTGA AGATGAAAGA 
GAATCCACAG ATGATGAGAG CAATCCTCTA GGCAGAGCTG GTATTTGGAC AAAGACTCAT 
ACAATATGGT ATAAACCTGT GAGAGAGGAT GAAGAAAGTA ATAAAGATTG TGTTGGTGGT 
AAAAGAGGAA GAGCCCAAAC AGCTCCAACG AAAACTTCCC CTAGAAATGC AAAAAAGCAT 
GATGAGTTAT GGCACGATGG AGTGTGCCCA TCAGTATCAA ATCCTTTAGA AGTTTACCTC 
ATTCCCACAC CACCTGAAAA TATAACATTT GAAGACCCGT CATTAGATGT GATCCTTCTT 
TTAAGAGTTT TACATGCTAT CAGTCGATAC TGGTATTACT TGTATGATAA TGCAATGTGC 
AAGGAAATTA TTCCAACTAG TGAATTTATT AACAGTAAGT TAACAGCAAA AGCAAATAGG 
CAACTTCAAG ATCCTTTAGT AATCATGACA GGAAACATCC CAACATGGCT TACTGAGCTA 
GGAAAAACCT GCCCATTTTT CTTTCCTTTT GATACCCGGC AAATGCTTTT TTATGTAACT 
GCATTTGATC GGGACCGAGC AATGCAAAGA TTACTTGATA CCAACCCAGA AATCAACCAG 
TCTGATTCTC AAGATAGCAG AGTTGCACCT AGATTGGATA GAAAAAAACG TACTGTGAAC 
CGAGAGGAGC TGCTGAAACA GGCGGAGTCT GTGATGCAGG ACCTCGGCAG CTCACGGGCC 
ATGTTAGAAA TCCAGTATGA AAATGAGGTT GGTACAGGTC TTGGGCCTAC ACTGGAGTTT 
TATGCGCTTG TATCTCAGGA ACTACAGAGA GCTGACTTGG GTCTTTGGAG AGGTGAAGAA 
GTAACTCTTA GCAATCCAAA AGGGAGCCAA GAAGGGACCA AGTATATTCA AAACCTCCAG 
GGCCTGTTTG CGCTTCCCTT TGGTAGGACA GCAAAGCCAG CTCATATCGC AAAGGTTAAG 
ATGAAGTTTC GCTTCTTAGG AAAATTAATG GCCAAGGCTA TCATGGATTT CAGATTGGTG 
GACCTTCCCC TTGGCTTACC CTTTTATAAA TGGATGCTAC GGCAAGAAAC TTCACTGACA 
TCACACGATT TGTTTGACAT CGACCCAGTT GTAGCCAGAT CAGTTTATCA CCTAGAAGAC 
ATTGTCAGAC AGAAGAAAAG ACTTGAACAA GATAAATCCC AGACCAAAGA GAGTCTACAG 
TATGCATTAG AAACCTTGAC TATGAATGGC TGCTCAGTTG AAGATCTAGG ACTGGATTTC 
ACTCTGCCAG GGTTTCCCAA TATCGAACTG AAGAAAGGAG GGAAGGATAT ACCAGTCACT 
ATCCACAATT TAGAGGAGTA TCTAAGACTG GTTATATTCT GGGCACTAAA TGAAGGCGTT 
TCTAGGCAAT TTGATTCGTT CAGAGATGGA TTTGAATCAG TCTTCCCACT CAGTCATCTT 
CAGTACTTCT ACCCGGAGGA ACTGGATCAG CTCCTTTGTG GCAGTAAAGC AGACACTTGG 
GATGCAAAGA CACTGATGGA ATGCTGTAGG CCTGATCATG GTTATACTCA TGACAGTCGG 
GCTGTGAAGT TTTTGTTTGA GATTCTCAGT AGTTTTGATA ATGAGCAGCA GAGGTTATTT 
CTCCAGTTTG TGACTGGTAG CCCAAGATTG CCTGTTGGAG GATTCCGGAG TTTGAATCCA 
CCTTTGACAA TTGTCCGAAA GACGTTTGAA TCAACAGAAA ACCCAGATGA CTTCTTGCCC 
TCTGTAATGA CTTGTGTGAA CTATCTTAAG TTGCCGGACT ATTCAAGCAT TGAGATAATG 
CGTGAAAAAC TGTTGATAGC AGCAAGAGAA GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348320.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335249.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 10, mRNA.

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CloneID	OHu102139
Clone ID Related Accession (Same CDS sequence)	NM_001348320.1 , NM_001348320.2 , NM_001348319.1
Accession Version	NM_001348320.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005280.1. Transcript Variant: This variant (10), as well as variant 9, encodes isoform f. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.221202.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335249.1
CDS	168..6356
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348320.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348320.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348320.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335249.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 10, mRNA.

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CloneID	OHu102139
Clone ID Related Accession (Same CDS sequence)	NM_001348320.1 , NM_001348320.2 , NM_001348319.1
Accession Version	NM_001348320.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348320.1. Transcript Variant: This variant (10), as well as variant 9, encodes isoform f. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.221202.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335249.1
CDS	569..6757
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348320.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348320.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348319.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335248.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 9, mRNA.

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CloneID	OHu102139
Clone ID Related Accession (Same CDS sequence)	NM_001348320.1 , NM_001348320.2 , NM_001348319.1
Accession Version	NM_001348319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6189bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005279.1. Transcript Variant: This variant (9), as well as variant 10, encodes isoform f. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.215138.1, SRR1803614.264109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335248.1
CDS	180..6368
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348319.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GCTGCTCAGT TCCTAAATTC AGGAAATGTA AGACACAGGC TGCAGTTTTA TATTGGAGAA 
CATTTGCTGC CGTATAACAT GACTGTGTAT CAGGCAGTAC GGCAGTTTAG TATACAGGCT 
GAAGATGAAA GAGAATCCAC AGATGATGAG AGCAATCCTC TAGGCAGAGC TGGTATTTGG 
ACAAAGACTC ATACAATATG GTATAAACCT GTGAGAGAGG ATGAAGAAAG TAATAAAGAT 
TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA ACAGCTCCAA CGAAAACTTC CCCTAGAAAT 
GCAAAAAAGC ATGATGAGTT ATGGCACGAT GGAGTGTGCC CATCAGTATC AAATCCTTTA 
GAAGTTTACC TCATTCCCAC ACCACCTGAA AATATAACAT TTGAAGACCC GTCATTAGAT 
GTGATCCTTC TTTTAAGAGT TTTACATGCT ATCAGTCGAT ACTGGTATTA CTTGTATGAT 
AATGCAATGT GCAAGGAAAT TATTCCAACT AGTGAATTTA TTAACAGTAA GTTAACAGCA 
AAAGCAAATA GGCAACTTCA AGATCCTTTA GTAATCATGA CAGGAAACAT CCCAACATGG 
CTTACTGAGC TAGGAAAAAC CTGCCCATTT TTCTTTCCTT TTGATACCCG GCAAATGCTT 
TTTTATGTAA CTGCATTTGA TCGGGACCGA GCAATGCAAA GATTACTTGA TACCAACCCA 
GAAATCAACC AGTCTGATTC TCAAGATAGC AGAGTTGCAC CTAGATTGGA TAGAAAAAAA 
CGTACTGTGA ACCGAGAGGA GCTGCTGAAA CAGGCGGAGT CTGTGATGCA GGACCTCGGC 
AGCTCACGGG CCATGTTAGA AATCCAGTAT GAAAATGAGG TTGGTACAGG TCTTGGGCCT 
ACACTGGAGT TTTATGCGCT TGTATCTCAG GAACTACAGA GAGCTGACTT GGGTCTTTGG 
AGAGGTGAAG AAGTAACTCT TAGCAATCCA AAAGGGAGCC AAGAAGGGAC CAAGTATATT 
CAAAACCTCC AGGGCCTGTT TGCGCTTCCC TTTGGTAGGA CAGCAAAGCC AGCTCATATC 
GCAAAGGTTA AGATGAAGTT TCGCTTCTTA GGAAAATTAA TGGCCAAGGC TATCATGGAT 
TTCAGATTGG TGGACCTTCC CCTTGGCTTA CCCTTTTATA AATGGATGCT ACGGCAAGAA 
ACTTCACTGA CATCACACGA TTTGTTTGAC ATCGACCCAG TTGTAGCCAG ATCAGTTTAT 
CACCTAGAAG ACATTGTCAG ACAGAAGAAA AGACTTGAAC AAGATAAATC CCAGACCAAA 
GAGAGTCTAC AGTATGCATT AGAAACCTTG ACTATGAATG GCTGCTCAGT TGAAGATCTA 
GGACTGGATT TCACTCTGCC AGGGTTTCCC AATATCGAAC TGAAGAAAGG AGGGAAGGAT 
ATACCAGTCA CTATCCACAA TTTAGAGGAG TATCTAAGAC TGGTTATATT CTGGGCACTA 
AATGAAGGCG TTTCTAGGCA ATTTGATTCG TTCAGAGATG GATTTGAATC AGTCTTCCCA 
CTCAGTCATC TTCAGTACTT CTACCCGGAG GAACTGGATC AGCTCCTTTG TGGCAGTAAA 
GCAGACACTT GGGATGCAAA GACACTGATG GAATGCTGTA GGCCTGATCA TGGTTATACT 
CATGACAGTC GGGCTGTGAA GTTTTTGTTT GAGATTCTCA GTAGTTTTGA TAATGAGCAG 
CAGAGGTTAT TTCTCCAGTT TGTGACTGGT AGCCCAAGAT TGCCTGTTGG AGGATTCCGG 
AGTTTGAATC CACCTTTGAC AATTGTCCGA AAGACGTTTG AATCAACAGA AAACCCAGAT 
GACTTCTTGC CCTCTGTAAT GACTTGTGTG AACTATCTTA AGTTGCCGGA CTATTCAAGC 
ATTGAGATAA TGCGTGAAAA ACTGTTGATA GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT 
CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348318.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335247.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 8, mRNA.

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CloneID	OHu102192
Clone ID Related Accession (Same CDS sequence)	NM_001348318.1 , NM_001348317.1 , NM_001348318.2
Accession Version	NM_001348318.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005288.1. Transcript Variant: This variant (8), as well as variant 7, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.13067.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335247.1
CDS	168..6230
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348318.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348317.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335246.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 7, mRNA.

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CloneID	OHu102192
Clone ID Related Accession (Same CDS sequence)	NM_001348318.1 , NM_001348317.1 , NM_001348318.2
Accession Version	NM_001348317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005287.1. Transcript Variant: This variant (7), as well as variant 8, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.57200.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335246.1
CDS	180..6242
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348317.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348318.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335247.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 8, mRNA.

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CloneID	OHu102192
Clone ID Related Accession (Same CDS sequence)	NM_001348318.1 , NM_001348317.1 , NM_001348318.2
Accession Version	NM_001348318.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348318.1. Transcript Variant: This variant (8), as well as variant 7, encodes isoform e. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.13067.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335247.1
CDS	569..6631
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348318.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348318.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348332.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335261.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 22, mRNA.

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CloneID	OHu102232
Clone ID Related Accession (Same CDS sequence)	NM_001348332.1
Accession Version	NM_001348332.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform k
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005284.1. Transcript Variant: This variant (22) encodes isoform k. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.63830.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAATT AAAATGCCAG TTACAAAGGC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCTCTGG CTTTGGTACA AGCCATCGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGCGATGAC 
GATGGATCAG ATGAGGAAAT AGATGAGTCT CTGGCTGCTC AGTTCCTAAA TTCAGGAAAT 
GTAAGACACA GGCTGCAGTT TTATATTGGA GAACATTTGC TGCCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT TAGTATACAG GCTGAAGATG AAAGAGAATC CACAGATGAT 
GAGAGCAATC CTCTAGGCAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTATAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACAGCTC CAACGAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAC 
GATGGAGTGT GCCCATCAGT ATCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATAA CATTTGAAGA CCCGTCATTA GATGTGATCC TTCTTTTAAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA AATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTAACA GCAAAAGCAA ATAGGCAACT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTTACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGGCAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACCGAGA GGAGCTGCTG 
AAACAGGCGG AGTCTGTGAT GCAGGACCTC GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC GCTTGTATCT 
CAGGAACTAC AGAGAGCTGA CTTGGGTCTT TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGGA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCAAA GCCAGCTCAT ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGC 
TTACCCTTTT ATAAATGGAT GCTACGGCAA GAAACTTCAC TGACATCACA CGATTTGTTT 
GACATCGACC CAGTTGTAGC CAGATCAGTT TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TACAGTATGC ATTAGAAACC 
TTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGACTGG ATTTCACTCT GCCAGGGTTT 
CCCAATATCG AACTGAAGAA AGGAGGGAAG GATATACCAG TCACTATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCTAG GCAATTTGAT 
TCGTTCAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTTCAGTA CTTCTACCCG 
GAGGAACTGG ATCAGCTCCT TTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GTAGGCCTGA TCATGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTTTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TATTTCTCCA GTTTGTGACT 
GGTAGCCCAA GATTGCCTGT TGGAGGATTC CGGAGTTTGA ATCCACCTTT GACAATTGTC 
CGAAAGACGT TTGAATCAAC AGAAAACCCA GATGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCATTGAGA TAATGCGTGA AAAACTGTTG 
ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335261.1
CDS	180..6281
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348332.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348332.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG CAGCCCATCA GGTCGGTGAG GATGAGATAA GCTTGTCCAC TCTGGGACGA 
GTTTATACTA TTGATTTTAA TTCTATGCAG CAAATCAATG AGGACACGGG AACAGCACGT 
GCCATTCAGA GAAAACCTAA CCCGTTAGCC AATAGTAACA CTAGTGGATA TTCAGAGTCA 
AAGAAGGATG ATGCTCGAGC ACAGCTTATG AAAGAGGATC CGGAACTGGC TAAGTCTTTT 
ATTAAGACAT TATTTGGTGT TCTTTATGAA GTGTATAGTT CCTCAGCAGG ACCTGCGGTC 
AGACATAAGT GCCTTAGAGC AATTCTTAGG ATAATTTATT TTGCGGATGC TGAACTTCTG 
AAGGATGTTC TGAAAAATCA TGCTGTTTCA AGTCACATTG CTTCCATGCT GTCAAGCCAA 
GACCTGAAGA TAGTAGTGGG AGCACTTCAG ATGGCAGAAA TTTTAATGCA GAAGTTACCT 
GATATTTTTA GTGTTTACTT CAGAAGAGAA GGTGTAATGC ATCAAGTAAA ACACTTAGCA 
GAATCAGAGT CTTTGTTGAC AAGTCCACCA AAGGCATGTA CGAATGGATC GGGATCCATG 
GGATCCACAA CTTCAGTCAG CAGTGGGACA GCCACAGCTG CCACTCATGC TGCAGCTGAC 
TTGGGATCAC CCAGCTTGCA GCACAGCAGG GATGATTCTT TAGATCTCAG CCCTCAAGGT 
CGATTAAGTG ATGTTCTAAA GAGAAAACGA CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG 
TACTCACCTC CAAGAGATGA TGACAAAGTA GACAATCAAG CTAAAAGCCC CACCACTACT 
CAGTCACCTA AATCTTCTTT CCTGGCAAGC TTGAATCCAA AAACATGGGG AAGGTTAAGT 
ACACAGTCCA ACAGCAACAA CATTGAGCCA GCACGGACTG CGGGAGGTAG TGGCCTTGCC 
AGGGCTGCCT CAAAGGATAC CATCTCCAAT AATAGAGAAA AAATTAAAGG TTGGATTAAG 
GAGCAGGCAC ATAAATTTGT AGAACGTTAT TTCAGTTCTG AGAATATGGA TGGAAGCAAC 
CCTGCATTGA ATGTCCTTCA GAGACTTTGT GCTGCAACCG AACAACTCAA CCTCCAGGTG 
GATGGTGGAG CTGAGTGCCT TGTAGAAATC CGTAGCATAG TCTCAGAGTC AGATGTTTCA 
TCATTTGAAA TCCAACATAG TGGATTTGTG AAGCAGCTGT TGCTTTATTT GACATCTAAA 
AGTGAAAAGG ATGCTGTGAG CAGAGAGATC AGATTAAAGC GATTTCTTCA TGTATTTTTT 
TCTTCTCCAC TTCCTGGAGA AGAGCCCATT GGAAGAGTGG AACCAGTGGG TAATGCACCT 
TTGTTGGCAT TAGTTCACAA GATGAACAAC TGCCTCAGCC AGATGGAACA ATTTCCAGTC 
AAAGTACATG ATTTCCCTAG TGGAAATGGG ACAGGAGGCA GAGGATCACA GGCTTTAAAA 
TTTTTCAACA CACATCAATT AAAATGCCAG TTACAAAGGC ATCCAGACTG TGCAAATGTG 
AAGCAGTGGA AGGGTGGACC TGTCAAGATT GACCCTCTGG CTTTGGTACA AGCCATCGAG 
AGATACCTTG TAGTTAGAGG GTATGGAAGA GTAAGAGAAG ATGATGAAGA CAGCGATGAC 
GATGGATCAG ATGAGGAAAT AGATGAGTCT CTGGCTGCTC AGTTCCTAAA TTCAGGAAAT 
GTAAGACACA GGCTGCAGTT TTATATTGGA GAACATTTGC TGCCGTATAA CATGACTGTG 
TATCAGGCAG TACGGCAGTT TAGTATACAG GCTGAAGATG AAAGAGAATC CACAGATGAT 
GAGAGCAATC CTCTAGGCAG AGCTGGTATT TGGACAAAGA CTCATACAAT ATGGTATAAA 
CCTGTGAGAG AGGATGAAGA AAGTAATAAA GATTGTGTTG GTGGTAAAAG AGGAAGAGCC 
CAAACAGCTC CAACGAAAAC TTCCCCTAGA AATGCAAAAA AGCATGATGA GTTATGGCAC 
GATGGAGTGT GCCCATCAGT ATCAAATCCT TTAGAAGTTT ACCTCATTCC CACACCACCT 
GAAAATATAA CATTTGAAGA CCCGTCATTA GATGTGATCC TTCTTTTAAG AGTTTTACAT 
GCTATCAGTC GATACTGGTA TTACTTGTAT GATAATGCAA TGTGCAAGGA AATTATTCCA 
ACTAGTGAAT TTATTAACAG TAAGTTAACA GCAAAAGCAA ATAGGCAACT TCAAGATCCT 
TTAGTAATCA TGACAGGAAA CATCCCAACA TGGCTTACTG AGCTAGGAAA AACCTGCCCA 
TTTTTCTTTC CTTTTGATAC CCGGCAAATG CTTTTTTATG TAACTGCATT TGATCGGGAC 
CGAGCAATGC AAAGATTACT TGATACCAAC CCAGAAATCA ACCAGTCTGA TTCTCAAGAT 
AGCAGAGTTG CACCTAGATT GGATAGAAAA AAACGTACTG TGAACCGAGA GGAGCTGCTG 
AAACAGGCGG AGTCTGTGAT GCAGGACCTC GGCAGCTCAC GGGCCATGTT AGAAATCCAG 
TATGAAAATG AGGTTGGTAC AGGTCTTGGG CCTACACTGG AGTTTTATGC GCTTGTATCT 
CAGGAACTAC AGAGAGCTGA CTTGGGTCTT TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT 
CCAAAAGGGA GCCAAGAAGG GACCAAGTAT ATTCAAAACC TCCAGGGCCT GTTTGCGCTT 
CCCTTTGGTA GGACAGCAAA GCCAGCTCAT ATCGCAAAGG TTAAGATGAA GTTTCGCTTC 
TTAGGAAAAT TAATGGCCAA GGCTATCATG GATTTCAGAT TGGTGGACCT TCCCCTTGGC 
TTACCCTTTT ATAAATGGAT GCTACGGCAA GAAACTTCAC TGACATCACA CGATTTGTTT 
GACATCGACC CAGTTGTAGC CAGATCAGTT TATCACCTAG AAGACATTGT CAGACAGAAG 
AAAAGACTTG AACAAGATAA ATCCCAGACC AAAGAGAGTC TACAGTATGC ATTAGAAACC 
TTGACTATGA ATGGCTGCTC AGTTGAAGAT CTAGGACTGG ATTTCACTCT GCCAGGGTTT 
CCCAATATCG AACTGAAGAA AGGAGGGAAG GATATACCAG TCACTATCCA CAATTTAGAG 
GAGTATCTAA GACTGGTTAT ATTCTGGGCA CTAAATGAAG GCGTTTCTAG GCAATTTGAT 
TCGTTCAGAG ATGGATTTGA ATCAGTCTTC CCACTCAGTC ATCTTCAGTA CTTCTACCCG 
GAGGAACTGG ATCAGCTCCT TTGTGGCAGT AAAGCAGACA CTTGGGATGC AAAGACACTG 
ATGGAATGCT GTAGGCCTGA TCATGGTTAT ACTCATGACA GTCGGGCTGT GAAGTTTTTG 
TTTGAGATTC TCAGTAGTTT TGATAATGAG CAGCAGAGGT TATTTCTCCA GTTTGTGACT 
GGTAGCCCAA GATTGCCTGT TGGAGGATTC CGGAGTTTGA ATCCACCTTT GACAATTGTC 
CGAAAGACGT TTGAATCAAC AGAAAACCCA GATGACTTCT TGCCCTCTGT AATGACTTGT 
GTGAACTATC TTAAGTTGCC GGACTATTCA AGCATTGAGA TAATGCGTGA AAAACTGTTG 
ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348323.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335252.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 13, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348323.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Sep 19, 2019 this sequence version replaced NM_001348323.1. Transcript Variant: This variant (13), as well as variants 12 and 14-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.71354.1, SRR1660807.112480.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335252.1
CDS	180..6383
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348323.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348323.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453224.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308992.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OHu104997
Clone ID Related Accession (Same CDS sequence)	XM_024453224.1 , XM_024453225.1 , XM_024453223.1 , XM_024453226.1 , XM_024453228.1 , XM_024453227.1
Accession Version	XM_024453224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308992.1
CDS	208..6486
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453224.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453224.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453225.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308993.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

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CloneID	OHu104997
Clone ID Related Accession (Same CDS sequence)	XM_024453224.1 , XM_024453225.1 , XM_024453223.1 , XM_024453226.1 , XM_024453228.1 , XM_024453227.1
Accession Version	XM_024453225.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308993.1
CDS	132..6410
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453225.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453225.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453223.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308991.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

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CloneID	OHu104997
Clone ID Related Accession (Same CDS sequence)	XM_024453224.1 , XM_024453225.1 , XM_024453223.1 , XM_024453226.1 , XM_024453228.1 , XM_024453227.1
Accession Version	XM_024453223.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308991.1
CDS	204..6482
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453223.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453223.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453226.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308994.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OHu104997
Clone ID Related Accession (Same CDS sequence)	XM_024453224.1 , XM_024453225.1 , XM_024453223.1 , XM_024453226.1 , XM_024453228.1 , XM_024453227.1
Accession Version	XM_024453226.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308994.1
CDS	157..6435
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453226.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453226.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453228.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308996.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

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CloneID	OHu104997
Clone ID Related Accession (Same CDS sequence)	XM_024453224.1 , XM_024453225.1 , XM_024453223.1 , XM_024453226.1 , XM_024453228.1 , XM_024453227.1
Accession Version	XM_024453228.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308996.1
CDS	78..6356
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453228.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453228.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453227.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308995.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OHu104997
Clone ID Related Accession (Same CDS sequence)	XM_024453224.1 , XM_024453225.1 , XM_024453223.1 , XM_024453226.1 , XM_024453228.1 , XM_024453227.1
Accession Version	XM_024453227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6279bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308995.1
CDS	265..6543
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453227.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGTAAGGT ACTATATTCC CACCATGTAT AATGTACTAT ATAAAGTTCA GGGCCTTTGG 
CTTAAATGGC TTTTGCTTTG CTTTATTCAG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453229.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308997.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OHu104998
Clone ID Related Accession (Same CDS sequence)	XM_024453229.1 , XM_024453230.1
Accession Version	XM_024453229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GTAAGGTACT ATATTCCCAC CATGTATAAT GTACTATATA AAGTTCAGGG CCTTTGGCTT 
AAATGGCTTT TGCTTTGCTT TATTCAGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308997.1
CDS	204..6479
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453229.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453229.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GTAAGGTACT ATATTCCCAC CATGTATAAT GTACTATATA AAGTTCAGGG CCTTTGGCTT 
AAATGGCTTT TGCTTTGCTT TATTCAGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453230.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308998.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

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CloneID	OHu104998
Clone ID Related Accession (Same CDS sequence)	XM_024453229.1 , XM_024453230.1
Accession Version	XM_024453230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GTAAGGTACT ATATTCCCAC CATGTATAAT GTACTATATA AAGTTCAGGG CCTTTGGCTT 
AAATGGCTTT TGCTTTGCTT TATTCAGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308998.1
CDS	141..6416
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453230.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453230.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGAG GATCACAGGC TTTAAAATTT TTCAACACAC ATCAATTAAA ATGCCAGTTA 
CAAAGGCATC CAGACTGTGC AAATGTGAAG CAGTGGAAGG GTGGACCTGT CAAGATTGAC 
CCTCTGGCTT TGGTACAAGC CATCGAGAGA TACCTTGTAG TTAGAGGGTA TGGAAGAGTA 
AGAGAAGATG ATGAAGACAG CGATGACGAT GGATCAGATG AGGAAATAGA TGAGTCTCTG 
GTAAGGTACT ATATTCCCAC CATGTATAAT GTACTATATA AAGTTCAGGG CCTTTGGCTT 
AAATGGCTTT TGCTTTGCTT TATTCAGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453231.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024308999.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

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CloneID	OHu104999
Clone ID Related Accession (Same CDS sequence)	XM_024453231.1
Accession Version	XM_024453231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCGTTAGCCA ATAGTAACAC TAGTGGATAT 
TCAGAGTCAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA GCACTTCAGA TGGCAGAAAT TTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGATCG 
GGATCCATGG GATCCACAAC TTCAGTCAGC AGTGGGACAG CCACAGCTGC CACTCATGCT 
GCAGCTGACT TGGGATCACC CAGCTTGCAG CACAGCAGGG ATGATTCTTT AGATCTCAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ACTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGTA CACAGTCCAA CAGCAACAAC ATTGAGCCAG CACGGACTGC GGGAGGTAGT 
GGCCTTGCCA GGGCTGCCTC AAAGGATACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAGG AGCAGGCACA TAAATTTGTA GAACGTTATT TCAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCAACCGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT GCTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC AGAGAGATCA GATTAAAGCG ATTTCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG GAAGAGTGGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGAAATGGGA CAGGAGGCAG AGGATCACAG 
GCTTTAAAAT TTTTCAACAC ACATCAATTA AAATGCCAGT TACAAAGGCA TCCAGACTGT 
GCAAATGTGA AGCAGTGGAA GGGTGGACCT GTCAAGATTG ACCCTCTGGC TTTGGTACAA 
GCCATCGAGA GATACCTTGT AGTTAGAGGG TATGGAAGAG TAAGAGAAGA TGATGAAGAC 
AGCGATGACG ATGGATCAGA TGAGGAAATA GATGAGTCTC TGGTAAGGTA CTATATTCCC 
ACCATGTATA ATGTACTATA TAAAGTTCAG GGCCTTTGGC TTAAATGGCT TTTGCTTTGC 
TTTATTCAGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024308999.1
CDS	204..6401
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453231.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453231.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCGTTAGCCA ATAGTAACAC TAGTGGATAT 
TCAGAGTCAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA GCACTTCAGA TGGCAGAAAT TTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGATCG 
GGATCCATGG GATCCACAAC TTCAGTCAGC AGTGGGACAG CCACAGCTGC CACTCATGCT 
GCAGCTGACT TGGGATCACC CAGCTTGCAG CACAGCAGGG ATGATTCTTT AGATCTCAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ACTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGTA CACAGTCCAA CAGCAACAAC ATTGAGCCAG CACGGACTGC GGGAGGTAGT 
GGCCTTGCCA GGGCTGCCTC AAAGGATACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAGG AGCAGGCACA TAAATTTGTA GAACGTTATT TCAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCAACCGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT GCTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC AGAGAGATCA GATTAAAGCG ATTTCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG GAAGAGTGGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGAAATGGGA CAGGAGGCAG AGGATCACAG 
GCTTTAAAAT TTTTCAACAC ACATCAATTA AAATGCCAGT TACAAAGGCA TCCAGACTGT 
GCAAATGTGA AGCAGTGGAA GGGTGGACCT GTCAAGATTG ACCCTCTGGC TTTGGTACAA 
GCCATCGAGA GATACCTTGT AGTTAGAGGG TATGGAAGAG TAAGAGAAGA TGATGAAGAC 
AGCGATGACG ATGGATCAGA TGAGGAAATA GATGAGTCTC TGGTAAGGTA CTATATTCCC 
ACCATGTATA ATGTACTATA TAAAGTTCAG GGCCTTTGGC TTAAATGGCT TTTGCTTTGC 
TTTATTCAGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453232.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309000.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

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CloneID	OHu105000
Clone ID Related Accession (Same CDS sequence)	XM_024453232.1
Accession Version	XM_024453232.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 
ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 
GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG TAAGGTACTA TATTCCCACC 
ATGTATAATG TACTATATAA AGTTCAGGGC CTTTGGCTTA AATGGCTTTT GCTTTGCTTT 
ATTCAGGCTG CTCAGTTCCT AAATTCAGGA AATGTAAGAC ACAGGCTGCA GTTTTATATT 
GGAGAACATT TGCTGCCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTTAGTATA 
CAGGCTGAAG ATGAAAGAGA ATCCACAGAT GATGAGAGCA ATCCTCTAGG CAGAGCTGGT 
ATTTGGACAA AGACTCATAC AATATGGTAT AAACCTGTGA GAGAGGATGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAAACAG CTCCAACGAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGTTATGG CACGATGGAG TGTGCCCATC AGTATCAAAT 
CCTTTAGAAG TTTACCTCAT TCCCACACCA CCTGAAAATA TAACATTTGA AGACCCGTCA 
TTAGATGTGA TCCTTCTTTT AAGAGTTTTA CATGCTATCA GTCGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAAATTATT CCAACTAGTG AATTTATTAA CAGTAAGTTA 
ACAGCAAAAG CAAATAGGCA ACTTCAAGAT CCTTTAGTAA TCATGACAGG AAACATCCCA 
ACATGGCTTA CTGAGCTAGG AAAAACCTGC CCATTTTTCT TTCCTTTTGA TACCCGGCAA 
ATGCTTTTTT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAAA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGATAGA 
AAAAAACGTA CTGTGAACCG AGAGGAGCTG CTGAAACAGG CGGAGTCTGT GATGCAGGAC 
CTCGGCAGCT CACGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG TACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCGCTTGTA TCTCAGGAAC TACAGAGAGC TGACTTGGGT 
CTTTGGAGAG GTGAAGAAGT AACTCTTAGC AATCCAAAAG GGAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAGGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC AAAGCCAGCT 
CATATCGCAA AGGTTAAGAT GAAGTTTCGC TTCTTAGGAA AATTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTT GGCTTACCCT TTTATAAATG GATGCTACGG 
CAAGAAACTT CACTGACATC ACACGATTTG TTTGACATCG ACCCAGTTGT AGCCAGATCA 
GTTTATCACC TAGAAGACAT TGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GTCTACAGTA TGCATTAGAA ACCTTGACTA TGAATGGCTG CTCAGTTGAA 
GATCTAGGAC TGGATTTCAC TCTGCCAGGG TTTCCCAATA TCGAACTGAA GAAAGGAGGG 
AAGGATATAC CAGTCACTAT CCACAATTTA GAGGAGTATC TAAGACTGGT TATATTCTGG 
GCACTAAATG AAGGCGTTTC TAGGCAATTT GATTCGTTCA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTTCA GTACTTCTAC CCGGAGGAAC TGGATCAGCT CCTTTGTGGC 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGTAGGCC TGATCATGGT 
TATACTCATG ACAGTCGGGC TGTGAAGTTT TTGTTTGAGA TTCTCAGTAG TTTTGATAAT 
GAGCAGCAGA GGTTATTTCT CCAGTTTGTG ACTGGTAGCC CAAGATTGCC TGTTGGAGGA 
TTCCGGAGTT TGAATCCACC TTTGACAATT GTCCGAAAGA CGTTTGAATC AACAGAAAAC 
CCAGATGACT TCTTGCCCTC TGTAATGACT TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCATTG AGATAATGCG TGAAAAACTG TTGATAGCAG CAAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309000.1
CDS	204..6398
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453232.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453232.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 
ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 
GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG TAAGGTACTA TATTCCCACC 
ATGTATAATG TACTATATAA AGTTCAGGGC CTTTGGCTTA AATGGCTTTT GCTTTGCTTT 
ATTCAGGCTG CTCAGTTCCT AAATTCAGGA AATGTAAGAC ACAGGCTGCA GTTTTATATT 
GGAGAACATT TGCTGCCGTA TAACATGACT GTGTATCAGG CAGTACGGCA GTTTAGTATA 
CAGGCTGAAG ATGAAAGAGA ATCCACAGAT GATGAGAGCA ATCCTCTAGG CAGAGCTGGT 
ATTTGGACAA AGACTCATAC AATATGGTAT AAACCTGTGA GAGAGGATGA AGAAAGTAAT 
AAAGATTGTG TTGGTGGTAA AAGAGGAAGA GCCCAAACAG CTCCAACGAA AACTTCCCCT 
AGAAATGCAA AAAAGCATGA TGAGTTATGG CACGATGGAG TGTGCCCATC AGTATCAAAT 
CCTTTAGAAG TTTACCTCAT TCCCACACCA CCTGAAAATA TAACATTTGA AGACCCGTCA 
TTAGATGTGA TCCTTCTTTT AAGAGTTTTA CATGCTATCA GTCGATACTG GTATTACTTG 
TATGATAATG CAATGTGCAA GGAAATTATT CCAACTAGTG AATTTATTAA CAGTAAGTTA 
ACAGCAAAAG CAAATAGGCA ACTTCAAGAT CCTTTAGTAA TCATGACAGG AAACATCCCA 
ACATGGCTTA CTGAGCTAGG AAAAACCTGC CCATTTTTCT TTCCTTTTGA TACCCGGCAA 
ATGCTTTTTT ATGTAACTGC ATTTGATCGG GACCGAGCAA TGCAAAGATT ACTTGATACC 
AACCCAGAAA TCAACCAGTC TGATTCTCAA GATAGCAGAG TTGCACCTAG ATTGGATAGA 
AAAAAACGTA CTGTGAACCG AGAGGAGCTG CTGAAACAGG CGGAGTCTGT GATGCAGGAC 
CTCGGCAGCT CACGGGCCAT GTTAGAAATC CAGTATGAAA ATGAGGTTGG TACAGGTCTT 
GGGCCTACAC TGGAGTTTTA TGCGCTTGTA TCTCAGGAAC TACAGAGAGC TGACTTGGGT 
CTTTGGAGAG GTGAAGAAGT AACTCTTAGC AATCCAAAAG GGAGCCAAGA AGGGACCAAG 
TATATTCAAA ACCTCCAGGG CCTGTTTGCG CTTCCCTTTG GTAGGACAGC AAAGCCAGCT 
CATATCGCAA AGGTTAAGAT GAAGTTTCGC TTCTTAGGAA AATTAATGGC CAAGGCTATC 
ATGGATTTCA GATTGGTGGA CCTTCCCCTT GGCTTACCCT TTTATAAATG GATGCTACGG 
CAAGAAACTT CACTGACATC ACACGATTTG TTTGACATCG ACCCAGTTGT AGCCAGATCA 
GTTTATCACC TAGAAGACAT TGTCAGACAG AAGAAAAGAC TTGAACAAGA TAAATCCCAG 
ACCAAAGAGA GTCTACAGTA TGCATTAGAA ACCTTGACTA TGAATGGCTG CTCAGTTGAA 
GATCTAGGAC TGGATTTCAC TCTGCCAGGG TTTCCCAATA TCGAACTGAA GAAAGGAGGG 
AAGGATATAC CAGTCACTAT CCACAATTTA GAGGAGTATC TAAGACTGGT TATATTCTGG 
GCACTAAATG AAGGCGTTTC TAGGCAATTT GATTCGTTCA GAGATGGATT TGAATCAGTC 
TTCCCACTCA GTCATCTTCA GTACTTCTAC CCGGAGGAAC TGGATCAGCT CCTTTGTGGC 
AGTAAAGCAG ACACTTGGGA TGCAAAGACA CTGATGGAAT GCTGTAGGCC TGATCATGGT 
TATACTCATG ACAGTCGGGC TGTGAAGTTT TTGTTTGAGA TTCTCAGTAG TTTTGATAAT 
GAGCAGCAGA GGTTATTTCT CCAGTTTGTG ACTGGTAGCC CAAGATTGCC TGTTGGAGGA 
TTCCGGAGTT TGAATCCACC TTTGACAATT GTCCGAAAGA CGTTTGAATC AACAGAAAAC 
CCAGATGACT TCTTGCCCTC TGTAATGACT TGTGTGAACT ATCTTAAGTT GCCGGACTAT 
TCAAGCATTG AGATAATGCG TGAAAAACTG TTGATAGCAG CAAGAGAAGG GCAGCAGTCG 
TTCCATCTTT CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453233.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309001.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

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CloneID	OHu105001
Clone ID Related Accession (Same CDS sequence)	XM_024453233.1 , XM_024453235.1 , XM_024453234.1
Accession Version	XM_024453233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGAGGATC ACAGGCTTTA 
AAATTTTTCA ACACACATCA ATTAAAATGC CAGTTACAAA GGCATCCAGA CTGTGCAAAT 
GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG ATTGACCCTC TGGCTTTGGT ACAAGCCATC 
GAGAGATACC TTGTAGTTAG AGGGTATGGA AGAGTAAGAG AAGATGATGA AGACAGCGAT 
GACGATGGAT CAGATGAGGA AATAGATGAG TCTCTGGTAA GGTACTATAT TCCCACCATG 
TATAATGTAC TATATAAAGT TCAGGGCCTT TGGCTTAAAT GGCTTTTGCT TTGCTTTATT 
CAGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309001.1
CDS	204..6395
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453233.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453233.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGAGGATC ACAGGCTTTA 
AAATTTTTCA ACACACATCA ATTAAAATGC CAGTTACAAA GGCATCCAGA CTGTGCAAAT 
GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG ATTGACCCTC TGGCTTTGGT ACAAGCCATC 
GAGAGATACC TTGTAGTTAG AGGGTATGGA AGAGTAAGAG AAGATGATGA AGACAGCGAT 
GACGATGGAT CAGATGAGGA AATAGATGAG TCTCTGGTAA GGTACTATAT TCCCACCATG 
TATAATGTAC TATATAAAGT TCAGGGCCTT TGGCTTAAAT GGCTTTTGCT TTGCTTTATT 
CAGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453235.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309003.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

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CloneID	OHu105001
Clone ID Related Accession (Same CDS sequence)	XM_024453233.1 , XM_024453235.1 , XM_024453234.1
Accession Version	XM_024453235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGAGGATC ACAGGCTTTA 
AAATTTTTCA ACACACATCA ATTAAAATGC CAGTTACAAA GGCATCCAGA CTGTGCAAAT 
GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG ATTGACCCTC TGGCTTTGGT ACAAGCCATC 
GAGAGATACC TTGTAGTTAG AGGGTATGGA AGAGTAAGAG AAGATGATGA AGACAGCGAT 
GACGATGGAT CAGATGAGGA AATAGATGAG TCTCTGGTAA GGTACTATAT TCCCACCATG 
TATAATGTAC TATATAAAGT TCAGGGCCTT TGGCTTAAAT GGCTTTTGCT TTGCTTTATT 
CAGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309003.1
CDS	70..6261
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453235.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453235.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGAGGATC ACAGGCTTTA 
AAATTTTTCA ACACACATCA ATTAAAATGC CAGTTACAAA GGCATCCAGA CTGTGCAAAT 
GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG ATTGACCCTC TGGCTTTGGT ACAAGCCATC 
GAGAGATACC TTGTAGTTAG AGGGTATGGA AGAGTAAGAG AAGATGATGA AGACAGCGAT 
GACGATGGAT CAGATGAGGA AATAGATGAG TCTCTGGTAA GGTACTATAT TCCCACCATG 
TATAATGTAC TATATAAAGT TCAGGGCCTT TGGCTTAAAT GGCTTTTGCT TTGCTTTATT 
CAGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453234.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309002.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

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CloneID	OHu105001
Clone ID Related Accession (Same CDS sequence)	XM_024453233.1 , XM_024453235.1 , XM_024453234.1
Accession Version	XM_024453234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGAGGATC ACAGGCTTTA 
AAATTTTTCA ACACACATCA ATTAAAATGC CAGTTACAAA GGCATCCAGA CTGTGCAAAT 
GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG ATTGACCCTC TGGCTTTGGT ACAAGCCATC 
GAGAGATACC TTGTAGTTAG AGGGTATGGA AGAGTAAGAG AAGATGATGA AGACAGCGAT 
GACGATGGAT CAGATGAGGA AATAGATGAG TCTCTGGTAA GGTACTATAT TCCCACCATG 
TATAATGTAC TATATAAAGT TCAGGGCCTT TGGCTTAAAT GGCTTTTGCT TTGCTTTATT 
CAGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309002.1
CDS	208..6399
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453234.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453234.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGAGGATC ACAGGCTTTA 
AAATTTTTCA ACACACATCA ATTAAAATGC CAGTTACAAA GGCATCCAGA CTGTGCAAAT 
GTGAAGCAGT GGAAGGGTGG ACCTGTCAAG ATTGACCCTC TGGCTTTGGT ACAAGCCATC 
GAGAGATACC TTGTAGTTAG AGGGTATGGA AGAGTAAGAG AAGATGATGA AGACAGCGAT 
GACGATGGAT CAGATGAGGA AATAGATGAG TCTCTGGTAA GGTACTATAT TCCCACCATG 
TATAATGTAC TATATAAAGT TCAGGGCCTT TGGCTTAAAT GGCTTTTGCT TTGCTTTATT 
CAGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453237.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309005.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

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CloneID	OHu105002
Clone ID Related Accession (Same CDS sequence)	XM_024453237.1 , XM_024453236.1 , XM_024453240.1 , XM_024453238.1 , XM_024453239.1
Accession Version	XM_024453237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309005.1
CDS	133..6285
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453237.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453237.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453236.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309004.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

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CloneID	OHu105002
Clone ID Related Accession (Same CDS sequence)	XM_024453237.1 , XM_024453236.1 , XM_024453240.1 , XM_024453238.1 , XM_024453239.1
Accession Version	XM_024453236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309004.1
CDS	180..6332
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453236.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453240.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309008.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

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CloneID	OHu105002
Clone ID Related Accession (Same CDS sequence)	XM_024453237.1 , XM_024453236.1 , XM_024453240.1 , XM_024453238.1 , XM_024453239.1
Accession Version	XM_024453240.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309008.1
CDS	167..6319
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453240.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453240.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453238.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309006.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

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CloneID	OHu105002
Clone ID Related Accession (Same CDS sequence)	XM_024453237.1 , XM_024453236.1 , XM_024453240.1 , XM_024453238.1 , XM_024453239.1
Accession Version	XM_024453238.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309006.1
CDS	229..6381
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453238.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453238.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309007.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.

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CloneID	OHu105002
Clone ID Related Accession (Same CDS sequence)	XM_024453237.1 , XM_024453236.1 , XM_024453240.1 , XM_024453238.1 , XM_024453239.1
Accession Version	XM_024453239.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309007.1
CDS	137..6289
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453239.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGTA 
AGGTACTATA TTCCCACCAT GTATAATGTA CTATATAAAG TTCAGGGCCT TTGGCTTAAA 
TGGCTTTTGC TTTGCTTTAT TCAGGCTGCT CAGTTCCTAA ATTCAGGAAA TGTAAGACAC 
AGGCTGCAGT TTTATATTGG AGAACATTTG CTGCCGTATA ACATGACTGT GTATCAGGCA 
GTACGGCAGT TTAGTATACA GGCTGAAGAT GAAAGAGAAT CCACAGATGA TGAGAGCAAT 
CCTCTAGGCA GAGCTGGTAT TTGGACAAAG ACTCATACAA TATGGTATAA ACCTGTGAGA 
GAGGATGAAG AAAGTAATAA AGATTGTGTT GGTGGTAAAA GAGGAAGAGC CCAAACAGCT 
CCAACGAAAA CTTCCCCTAG AAATGCAAAA AAGCATGATG AGTTATGGCA CGATGGAGTG 
TGCCCATCAG TATCAAATCC TTTAGAAGTT TACCTCATTC CCACACCACC TGAAAATATA 
ACATTTGAAG ACCCGTCATT AGATGTGATC CTTCTTTTAA GAGTTTTACA TGCTATCAGT 
CGATACTGGT ATTACTTGTA TGATAATGCA ATGTGCAAGG AAATTATTCC AACTAGTGAA 
TTTATTAACA GTAAGTTAAC AGCAAAAGCA AATAGGCAAC TTCAAGATCC TTTAGTAATC 
ATGACAGGAA ACATCCCAAC ATGGCTTACT GAGCTAGGAA AAACCTGCCC ATTTTTCTTT 
CCTTTTGATA CCCGGCAAAT GCTTTTTTAT GTAACTGCAT TTGATCGGGA CCGAGCAATG 
CAAAGATTAC TTGATACCAA CCCAGAAATC AACCAGTCTG ATTCTCAAGA TAGCAGAGTT 
GCACCTAGAT TGGATAGAAA AAAACGTACT GTGAACCGAG AGGAGCTGCT GAAACAGGCG 
GAGTCTGTGA TGCAGGACCT CGGCAGCTCA CGGGCCATGT TAGAAATCCA GTATGAAAAT 
GAGGTTGGTA CAGGTCTTGG GCCTACACTG GAGTTTTATG CGCTTGTATC TCAGGAACTA 
CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG 
AGCCAAGAAG GGACCAAGTA TATTCAAAAC CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT 
AGGACAGCAA AGCCAGCTCA TATCGCAAAG GTTAAGATGA AGTTTCGCTT CTTAGGAAAA 
TTAATGGCCA AGGCTATCAT GGATTTCAGA TTGGTGGACC TTCCCCTTGG CTTACCCTTT 
TATAAATGGA TGCTACGGCA AGAAACTTCA CTGACATCAC ACGATTTGTT TGACATCGAC 
CCAGTTGTAG CCAGATCAGT TTATCACCTA GAAGACATTG TCAGACAGAA GAAAAGACTT 
GAACAAGATA AATCCCAGAC CAAAGAGAGT CTACAGTATG CATTAGAAAC CTTGACTATG 
AATGGCTGCT CAGTTGAAGA TCTAGGACTG GATTTCACTC TGCCAGGGTT TCCCAATATC 
GAACTGAAGA AAGGAGGGAA GGATATACCA GTCACTATCC ACAATTTAGA GGAGTATCTA 
AGACTGGTTA TATTCTGGGC ACTAAATGAA GGCGTTTCTA GGCAATTTGA TTCGTTCAGA 
GATGGATTTG AATCAGTCTT CCCACTCAGT CATCTTCAGT ACTTCTACCC GGAGGAACTG 
GATCAGCTCC TTTGTGGCAG TAAAGCAGAC ACTTGGGATG CAAAGACACT GATGGAATGC 
TGTAGGCCTG ATCATGGTTA TACTCATGAC AGTCGGGCTG TGAAGTTTTT GTTTGAGATT 
CTCAGTAGTT TTGATAATGA GCAGCAGAGG TTATTTCTCC AGTTTGTGAC TGGTAGCCCA 
AGATTGCCTG TTGGAGGATT CCGGAGTTTG AATCCACCTT TGACAATTGT CCGAAAGACG 
TTTGAATCAA CAGAAAACCC AGATGACTTC TTGCCCTCTG TAATGACTTG TGTGAACTAT 
CTTAAGTTGC CGGACTATTC AAGCATTGAG ATAATGCGTG AAAAACTGTT GATAGCAGCA 
AGAGAAGGGC AGCAGTCGTT CCATCTTTCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453241.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309009.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

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CloneID	OHu105003
Clone ID Related Accession (Same CDS sequence)	XM_024453241.1
Accession Version	XM_024453241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGTAAGG 
TACTATATTC CCACCATGTA TAATGTACTA TATAAAGTTC AGGGCCTTTG GCTTAAATGG 
CTTTTGCTTT GCTTTATTCA GGCTGCTCAG TTCCTAAATT CAGGAAATGT AAGACACAGG 
CTGCAGTTTT ATATTGGAGA ACATTTGCTG CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTTA GTATACAGGC TGAAGATGAA AGAGAATCCA CAGATGATGA GAGCAATCCT 
CTAGGCAGAG CTGGTATTTG GACAAAGACT CATACAATAT GGTATAAACC TGTGAGAGAG 
GATGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA AACAGCTCCA 
ACGAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGT TATGGCACGA TGGAGTGTGC 
CCATCAGTAT CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATAACA 
TTTGAAGACC CGTCATTAGA TGTGATCCTT CTTTTAAGAG TTTTACATGC TATCAGTCGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAAA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AGTTAACAGC AAAAGCAAAT AGGCAACTTC AAGATCCTTT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTTACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GGCAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACCGAGAGG AGCTGCTGAA ACAGGCGGAG 
TCTGTGATGC AGGACCTCGG CAGCTCACGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGTACAG GTCTTGGGCC TACACTGGAG TTTTATGCGC TTGTATCTCA GGAACTACAG 
AGAGCTGACT TGGGTCTTTG GAGAGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGGAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCAAAGC CAGCTCATAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTTGGCTT ACCCTTTTAT 
AAATGGATGC TACGGCAAGA AACTTCACTG ACATCACACG ATTTGTTTGA CATCGACCCA 
GTTGTAGCCA GATCAGTTTA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGTCTA CAGTATGCAT TAGAAACCTT GACTATGAAT 
GGCTGCTCAG TTGAAGATCT AGGACTGGAT TTCACTCTGC CAGGGTTTCC CAATATCGAA 
CTGAAGAAAG GAGGGAAGGA TATACCAGTC ACTATCCACA ATTTAGAGGA GTATCTAAGA 
CTGGTTATAT TCTGGGCACT AAATGAAGGC GTTTCTAGGC AATTTGATTC GTTCAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTTCAGTACT TCTACCCGGA GGAACTGGAT 
CAGCTCCTTT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGT 
AGGCCTGATC ATGGTTATAC TCATGACAGT CGGGCTGTGA AGTTTTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTA TTTCTCCAGT TTGTGACTGG TAGCCCAAGA 
TTGCCTGTTG GAGGATTCCG GAGTTTGAAT CCACCTTTGA CAATTGTCCG AAAGACGTTT 
GAATCAACAG AAAACCCAGA TGACTTCTTG CCCTCTGTAA TGACTTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CATTGAGATA ATGCGTGAAA AACTGTTGAT AGCAGCAAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309009.1
CDS	180..6329
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453241.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453241.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGTAAGG 
TACTATATTC CCACCATGTA TAATGTACTA TATAAAGTTC AGGGCCTTTG GCTTAAATGG 
CTTTTGCTTT GCTTTATTCA GGCTGCTCAG TTCCTAAATT CAGGAAATGT AAGACACAGG 
CTGCAGTTTT ATATTGGAGA ACATTTGCTG CCGTATAACA TGACTGTGTA TCAGGCAGTA 
CGGCAGTTTA GTATACAGGC TGAAGATGAA AGAGAATCCA CAGATGATGA GAGCAATCCT 
CTAGGCAGAG CTGGTATTTG GACAAAGACT CATACAATAT GGTATAAACC TGTGAGAGAG 
GATGAAGAAA GTAATAAAGA TTGTGTTGGT GGTAAAAGAG GAAGAGCCCA AACAGCTCCA 
ACGAAAACTT CCCCTAGAAA TGCAAAAAAG CATGATGAGT TATGGCACGA TGGAGTGTGC 
CCATCAGTAT CAAATCCTTT AGAAGTTTAC CTCATTCCCA CACCACCTGA AAATATAACA 
TTTGAAGACC CGTCATTAGA TGTGATCCTT CTTTTAAGAG TTTTACATGC TATCAGTCGA 
TACTGGTATT ACTTGTATGA TAATGCAATG TGCAAGGAAA TTATTCCAAC TAGTGAATTT 
ATTAACAGTA AGTTAACAGC AAAAGCAAAT AGGCAACTTC AAGATCCTTT AGTAATCATG 
ACAGGAAACA TCCCAACATG GCTTACTGAG CTAGGAAAAA CCTGCCCATT TTTCTTTCCT 
TTTGATACCC GGCAAATGCT TTTTTATGTA ACTGCATTTG ATCGGGACCG AGCAATGCAA 
AGATTACTTG ATACCAACCC AGAAATCAAC CAGTCTGATT CTCAAGATAG CAGAGTTGCA 
CCTAGATTGG ATAGAAAAAA ACGTACTGTG AACCGAGAGG AGCTGCTGAA ACAGGCGGAG 
TCTGTGATGC AGGACCTCGG CAGCTCACGG GCCATGTTAG AAATCCAGTA TGAAAATGAG 
GTTGGTACAG GTCTTGGGCC TACACTGGAG TTTTATGCGC TTGTATCTCA GGAACTACAG 
AGAGCTGACT TGGGTCTTTG GAGAGGTGAA GAAGTAACTC TTAGCAATCC AAAAGGGAGC 
CAAGAAGGGA CCAAGTATAT TCAAAACCTC CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG 
ACAGCAAAGC CAGCTCATAT CGCAAAGGTT AAGATGAAGT TTCGCTTCTT AGGAAAATTA 
ATGGCCAAGG CTATCATGGA TTTCAGATTG GTGGACCTTC CCCTTGGCTT ACCCTTTTAT 
AAATGGATGC TACGGCAAGA AACTTCACTG ACATCACACG ATTTGTTTGA CATCGACCCA 
GTTGTAGCCA GATCAGTTTA TCACCTAGAA GACATTGTCA GACAGAAGAA AAGACTTGAA 
CAAGATAAAT CCCAGACCAA AGAGAGTCTA CAGTATGCAT TAGAAACCTT GACTATGAAT 
GGCTGCTCAG TTGAAGATCT AGGACTGGAT TTCACTCTGC CAGGGTTTCC CAATATCGAA 
CTGAAGAAAG GAGGGAAGGA TATACCAGTC ACTATCCACA ATTTAGAGGA GTATCTAAGA 
CTGGTTATAT TCTGGGCACT AAATGAAGGC GTTTCTAGGC AATTTGATTC GTTCAGAGAT 
GGATTTGAAT CAGTCTTCCC ACTCAGTCAT CTTCAGTACT TCTACCCGGA GGAACTGGAT 
CAGCTCCTTT GTGGCAGTAA AGCAGACACT TGGGATGCAA AGACACTGAT GGAATGCTGT 
AGGCCTGATC ATGGTTATAC TCATGACAGT CGGGCTGTGA AGTTTTTGTT TGAGATTCTC 
AGTAGTTTTG ATAATGAGCA GCAGAGGTTA TTTCTCCAGT TTGTGACTGG TAGCCCAAGA 
TTGCCTGTTG GAGGATTCCG GAGTTTGAAT CCACCTTTGA CAATTGTCCG AAAGACGTTT 
GAATCAACAG AAAACCCAGA TGACTTCTTG CCCTCTGTAA TGACTTGTGT GAACTATCTT 
AAGTTGCCGG ACTATTCAAG CATTGAGATA ATGCGTGAAA AACTGTTGAT AGCAGCAAGA 
GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_024453242.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309010.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.

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CloneID	OHu105004
Clone ID Related Accession (Same CDS sequence)	XM_024453242.1
Accession Version	XM_024453242.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC GAAGAAACGC 
AGTGAGTCTC CACCTGCTGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGGCAGT AAATTCTTCA 
GCTGCTCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCAGATGATT CCGAGATGGG ACGTTTGCAA 
GCTTTGTTAG AGGCAAGGGG TCTTCCCCCT CACCTATTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGT GGAGCTAGTT CTAAGGCCCA GCAGCTACTA 
CAAGGATTGC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CAGTTATTGA GATGTGTCAG 
TTACTGGTCA TGGGAAATGA GGAGACACTG GGAGGGTTTC CTGTCAAGAG TGTTGTTCCA 
GCTTTGATTA CGTTACTTCA GATGGAGCAC AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCTTCTG CTGTTGTAGT AGATGCTATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGAGACAT AGTAAAGCCA TTCTACAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTACCT AGAATTCTTC AGCATAAATG CCCAAAGAAA TGCATTAGCA 
ATTGCAGCTA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCAGATTCA 
CTCCCATTGC TAACCCAAAG GCTAACACAT CAGGATAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGCCTAGT GGACAACTTC CAGCATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTTAC AAATGTTCAA CAGCTGTTGG TAGTGACTCC ACCCATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACTTTAGCTG TTCAACTTAT GAAACAAAAC ATTGCAGAAA CGCTTCACTT TCTCCTGTGT 
GGTGCCTCCA ATGGAAGTTG TCAGGAACAG ATTGATCTTG TTCCACGAAG CCCTCAAGAG 
TTGTATGAAC TGACATCTCT GATTTGTGAA CTTATGCCAT GTTTACCAAA AGAAGGCATT 
TTTGCAGTTG ATACCATGTT GAAGAAGGGA AATGCACAGA ACACAGATGG TGCGATATGG 
CAGTGGCGTG ATGATCGGGG CCTCTGGCAT CCATATAACA GGATTGACAG CCGGATCATT 
GAGGTAGCAG CCCATCAGGT CGGTGAGGAT GAGATAAGCT TGTCCACTCT GGGACGAGTT 
TATACTATTG ATTTTAATTC TATGCAGCAA ATCAATGAGG ACACGGGAAC AGCACGTGCC 
ATTCAGAGAA AACCTAACCC GTTAGCCAAT AGTAACACTA GTGGATATTC AGAGTCAAAG 
AAGGATGATG CTCGAGCACA GCTTATGAAA GAGGATCCGG AACTGGCTAA GTCTTTTATT 
AAGACATTAT TTGGTGTTCT TTATGAAGTG TATAGTTCCT CAGCAGGACC TGCGGTCAGA 
CATAAGTGCC TTAGAGCAAT TCTTAGGATA ATTTATTTTG CGGATGCTGA ACTTCTGAAG 
GATGTTCTGA AAAATCATGC TGTTTCAAGT CACATTGCTT CCATGCTGTC AAGCCAAGAC 
CTGAAGATAG TAGTGGGAGC ACTTCAGATG GCAGAAATTT TAATGCAGAA GTTACCTGAT 
ATTTTTAGTG TTTACTTCAG AAGAGAAGGT GTAATGCATC AAGTAAAACA CTTAGCAGAA 
TCAGAGTCTT TGTTGACAAG TCCACCAAAG GCATGTACGA ATGGATCGGG ATCCATGGGA 
TCCACAACTT CAGTCAGCAG TGGGACAGCC ACAGCTGCCA CTCATGCTGC AGCTGACTTG 
GGATCACCCA GCTTGCAGCA CAGCAGGGAT GATTCTTTAG ATCTCAGCCC TCAAGGTCGA 
TTAAGTGATG TTCTAAAGAG AAAACGACTG CCAAAACGAG GGCCAAGAAG GCCAAAGTAC 
TCACCTCCAA GAGATGATGA CAAAGTAGAC AATCAAGCTA AAAGCCCCAC CACTACTCAG 
TCACCTAAAT CTTCTTTCCT GGCAAGCTTG AATCCAAAAA CATGGGGAAG GTTAAGTACA 
CAGTCCAACA GCAACAACAT TGAGCCAGCA CGGACTGCGG GAGGTAGTGG CCTTGCCAGG 
GCTGCCTCAA AGGATACCAT CTCCAATAAT AGAGAAAAAA TTAAAGGTTG GATTAAGGAG 
CAGGCACATA AATTTGTAGA ACGTTATTTC AGTTCTGAGA ATATGGATGG AAGCAACCCT 
GCATTGAATG TCCTTCAGAG ACTTTGTGCT GCAACCGAAC AACTCAACCT CCAGGTGGAT 
GGTGGAGCTG AGTGCCTTGT AGAAATCCGT AGCATAGTCT CAGAGTCAGA TGTTTCATCA 
TTTGAAATCC AACATAGTGG ATTTGTGAAG CAGCTGTTGC TTTATTTGAC ATCTAAAAGT 
GAAAAGGATG CTGTGAGCAG AGAGATCAGA TTAAAGCGAT TTCTTCATGT ATTTTTTTCT 
TCTCCACTTC CTGGAGAAGA GCCCATTGGA AGAGTGGAAC CAGTGGGTAA TGCACCTTTG 
TTGGCATTAG TTCACAAGAT GAACAACTGC CTCAGCCAGA TGGAACAATT TCCAGTCAAA 
GTACATGATT TCCCTAGTGG AAATGGGACA GGAGGCAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GTAAGGTACT ATATTCCCAC CATGTATAAT 
GTACTATATA AAGTTCAGGG CCTTTGGCTT AAATGGCTTT TGCTTTGCTT TATTCAGGCT 
GCTCAGTTCC TAAATTCAGG AAATGTAAGA CACAGGCTGC AGTTTTATAT TGGAGAACAT 
TTGCTGCCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTTAGTAT ACAGGCTGAA 
GATGAAAGAG AATCCACAGA TGATGAGAGC AATCCTCTAG GCAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA TAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACA GCTCCAACGA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCACGATGGA GTGTGCCCAT CAGTATCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATAACATTTG AAGACCCGTC ATTAGATGTG 
ATCCTTCTTT TAAGAGTTTT ACATGCTATC AGTCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAAATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT AACAGCAAAA 
GCAAATAGGC AACTTCAAGA TCCTTTAGTA ATCATGACAG GAAACATCCC AACATGGCTT 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGGCA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACC GAGAGGAGCT GCTGAAACAG GCGGAGTCTG TGATGCAGGA CCTCGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA CTACAGAGAG CTGACTTGGG TCTTTGGAGA 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGGAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CAAAGCCAGC TCATATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGCTTACCC TTTTATAAAT GGATGCTACG GCAAGAAACT 
TCACTGACAT CACACGATTT GTTTGACATC GACCCAGTTG TAGCCAGATC AGTTTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAGT ATGCATTAGA AACCTTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
CTGGATTTCA CTCTGCCAGG GTTTCCCAAT ATCGAACTGA AGAAAGGAGG GAAGGATATA 
CCAGTCACTA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CTAGGCAATT TGATTCGTTC AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTTC AGTACTTCTA CCCGGAGGAA CTGGATCAGC TCCTTTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGTAGGC CTGATCATGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT TTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTATTTC TCCAGTTTGT GACTGGTAGC CCAAGATTGC CTGTTGGAGG ATTCCGGAGT 
TTGAATCCAC CTTTGACAAT TGTCCGAAAG ACGTTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCATT 
GAGATAATGC GTGAAAAACT GTTGATAGCA GCAAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309010.1
CDS	180..6245
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLVRYYIPTMYNVLYKVQGLWLKWLLLCFIQAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_024453242.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453242.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGGTCTT CTAAGTCAGA GACATCAAAA 
CCTGGACCTT CTGGATTACA GGCCAAATTA GCAAGTTTAA GAAAATCTAC GAAGAAACGC 
AGTGAGTCTC CACCTGCTGA GCTCCCCAGT TTGAGGCGGA GCACACGCCA AAAGACCACG 
GGCTCCTGTG CTAGTACCAG TCGGCGAGGC TCTGGCCTGG GCAAAAGAGG AGCAGCTGAA 
GCTCGTCGAC AGGAGAAAAT GGCAGACCCT GAAAGCAACC AGGAGGCAGT AAATTCTTCA 
GCTGCTCGGA CAGATGAAGC TCCCCAAGGA GCTGCAGCCT CTAGTTCTGT TGCAGGGGCT 
GTTGGCATGA CCACCTCTGG GGAGAGTGAA TCAGATGATT CCGAGATGGG ACGTTTGCAA 
GCTTTGTTAG AGGCAAGGGG TCTTCCCCCT CACCTATTTG GTCCTCTTGG TCCTCGGATG 
TCACAGCTTT TCCATAGAAC AATTGGAAGT GGAGCTAGTT CTAAGGCCCA GCAGCTACTA 
CAAGGATTGC AAGCCAGTGA TGAAAGTCAA CAGCTTCAGG CAGTTATTGA GATGTGTCAG 
TTACTGGTCA TGGGAAATGA GGAGACACTG GGAGGGTTTC CTGTCAAGAG TGTTGTTCCA 
GCTTTGATTA CGTTACTTCA GATGGAGCAC AATTTTGATA TTATGAACCA TGCTTGTCGA 
GCCTTAACAT ACATGATGGA AGCACTTCCT CGATCTTCTG CTGTTGTAGT AGATGCTATT 
CCTGTCTTTT TAGAAAAGCT GCAAGTTATT CAGTGTATTG ATGTGGCAGA GCAGGCCTTG 
ACTGCCTTGG AGATGTTGTC ACGGAGACAT AGTAAAGCCA TTCTACAGGC GGGTGGTTTG 
GCAGACTGCT TGCTGTACCT AGAATTCTTC AGCATAAATG CCCAAAGAAA TGCATTAGCA 
ATTGCAGCTA ATTGCTGCCA GAGTATCACG CCAGATGAAT TTCATTTTGT GGCAGATTCA 
CTCCCATTGC TAACCCAAAG GCTAACACAT CAGGATAAAA AGTCAGTAGA AAGCACTTGC 
CTTTGTTTTG CACGCCTAGT GGACAACTTC CAGCATGAGG AGAATTTACT CCAGCAGGTT 
GCTTCCAAAG ATCTGCTTAC AAATGTTCAA CAGCTGTTGG TAGTGACTCC ACCCATTTTA 
AGTTCTGGGA TGTTTATAAT GGTGGTTCGC ATGTTTTCTC TGATGTGTTC CAACTGTCCA 
ACTTTAGCTG TTCAACTTAT GAAACAAAAC ATTGCAGAAA CGCTTCACTT TCTCCTGTGT 
GGTGCCTCCA ATGGAAGTTG TCAGGAACAG ATTGATCTTG TTCCACGAAG CCCTCAAGAG 
TTGTATGAAC TGACATCTCT GATTTGTGAA CTTATGCCAT GTTTACCAAA AGAAGGCATT 
TTTGCAGTTG ATACCATGTT GAAGAAGGGA AATGCACAGA ACACAGATGG TGCGATATGG 
CAGTGGCGTG ATGATCGGGG CCTCTGGCAT CCATATAACA GGATTGACAG CCGGATCATT 
GAGGTAGCAG CCCATCAGGT CGGTGAGGAT GAGATAAGCT TGTCCACTCT GGGACGAGTT 
TATACTATTG ATTTTAATTC TATGCAGCAA ATCAATGAGG ACACGGGAAC AGCACGTGCC 
ATTCAGAGAA AACCTAACCC GTTAGCCAAT AGTAACACTA GTGGATATTC AGAGTCAAAG 
AAGGATGATG CTCGAGCACA GCTTATGAAA GAGGATCCGG AACTGGCTAA GTCTTTTATT 
AAGACATTAT TTGGTGTTCT TTATGAAGTG TATAGTTCCT CAGCAGGACC TGCGGTCAGA 
CATAAGTGCC TTAGAGCAAT TCTTAGGATA ATTTATTTTG CGGATGCTGA ACTTCTGAAG 
GATGTTCTGA AAAATCATGC TGTTTCAAGT CACATTGCTT CCATGCTGTC AAGCCAAGAC 
CTGAAGATAG TAGTGGGAGC ACTTCAGATG GCAGAAATTT TAATGCAGAA GTTACCTGAT 
ATTTTTAGTG TTTACTTCAG AAGAGAAGGT GTAATGCATC AAGTAAAACA CTTAGCAGAA 
TCAGAGTCTT TGTTGACAAG TCCACCAAAG GCATGTACGA ATGGATCGGG ATCCATGGGA 
TCCACAACTT CAGTCAGCAG TGGGACAGCC ACAGCTGCCA CTCATGCTGC AGCTGACTTG 
GGATCACCCA GCTTGCAGCA CAGCAGGGAT GATTCTTTAG ATCTCAGCCC TCAAGGTCGA 
TTAAGTGATG TTCTAAAGAG AAAACGACTG CCAAAACGAG GGCCAAGAAG GCCAAAGTAC 
TCACCTCCAA GAGATGATGA CAAAGTAGAC AATCAAGCTA AAAGCCCCAC CACTACTCAG 
TCACCTAAAT CTTCTTTCCT GGCAAGCTTG AATCCAAAAA CATGGGGAAG GTTAAGTACA 
CAGTCCAACA GCAACAACAT TGAGCCAGCA CGGACTGCGG GAGGTAGTGG CCTTGCCAGG 
GCTGCCTCAA AGGATACCAT CTCCAATAAT AGAGAAAAAA TTAAAGGTTG GATTAAGGAG 
CAGGCACATA AATTTGTAGA ACGTTATTTC AGTTCTGAGA ATATGGATGG AAGCAACCCT 
GCATTGAATG TCCTTCAGAG ACTTTGTGCT GCAACCGAAC AACTCAACCT CCAGGTGGAT 
GGTGGAGCTG AGTGCCTTGT AGAAATCCGT AGCATAGTCT CAGAGTCAGA TGTTTCATCA 
TTTGAAATCC AACATAGTGG ATTTGTGAAG CAGCTGTTGC TTTATTTGAC ATCTAAAAGT 
GAAAAGGATG CTGTGAGCAG AGAGATCAGA TTAAAGCGAT TTCTTCATGT ATTTTTTTCT 
TCTCCACTTC CTGGAGAAGA GCCCATTGGA AGAGTGGAAC CAGTGGGTAA TGCACCTTTG 
TTGGCATTAG TTCACAAGAT GAACAACTGC CTCAGCCAGA TGGAACAATT TCCAGTCAAA 
GTACATGATT TCCCTAGTGG AAATGGGACA GGAGGCAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GTAAGGTACT ATATTCCCAC CATGTATAAT 
GTACTATATA AAGTTCAGGG CCTTTGGCTT AAATGGCTTT TGCTTTGCTT TATTCAGGCT 
GCTCAGTTCC TAAATTCAGG AAATGTAAGA CACAGGCTGC AGTTTTATAT TGGAGAACAT 
TTGCTGCCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTTAGTAT ACAGGCTGAA 
GATGAAAGAG AATCCACAGA TGATGAGAGC AATCCTCTAG GCAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA TAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACA GCTCCAACGA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCACGATGGA GTGTGCCCAT CAGTATCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATAACATTTG AAGACCCGTC ATTAGATGTG 
ATCCTTCTTT TAAGAGTTTT ACATGCTATC AGTCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAAATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT AACAGCAAAA 
GCAAATAGGC AACTTCAAGA TCCTTTAGTA ATCATGACAG GAAACATCCC AACATGGCTT 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGGCA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACC GAGAGGAGCT GCTGAAACAG GCGGAGTCTG TGATGCAGGA CCTCGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA CTACAGAGAG CTGACTTGGG TCTTTGGAGA 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGGAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CAAAGCCAGC TCATATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGCTTACCC TTTTATAAAT GGATGCTACG GCAAGAAACT 
TCACTGACAT CACACGATTT GTTTGACATC GACCCAGTTG TAGCCAGATC AGTTTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAGT ATGCATTAGA AACCTTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
CTGGATTTCA CTCTGCCAGG GTTTCCCAAT ATCGAACTGA AGAAAGGAGG GAAGGATATA 
CCAGTCACTA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CTAGGCAATT TGATTCGTTC AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTTC AGTACTTCTA CCCGGAGGAA CTGGATCAGC TCCTTTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGTAGGC CTGATCATGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT TTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTATTTC TCCAGTTTGT GACTGGTAGC CCAAGATTGC CTGTTGGAGG ATTCCGGAGT 
TTGAATCCAC CTTTGACAAT TGTCCGAAAG ACGTTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCATT 
GAGATAATGC GTGAAAAACT GTTGATAGCA GCAAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005283.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860772.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

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CloneID	OHu92731
Clone ID Related Accession (Same CDS sequence)	XM_017005283.1 , XM_017005283.2
Accession Version	XM_017005283.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 
ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 
GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG CTGCTCAGTT CCTAAATTCA 
GGAAATGTAA GACACAGGCT GCAGTTTTAT ATTGGAGAAC ATTTGCTGCC GTATAACATG 
ACTGTGTATC AGGCAGTACG GCAGTTTAGT ATACAGGCTG AAGATGAAAG AGAATCCACA 
GATGATGAGA GCAATCCTCT AGGCAGAGCT GGTATTTGGA CAAAGACTCA TACAATATGG 
TATAAACCTG TGAGAGAGGA TGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 
AGAGCCCAAA CAGCTCCAAC GAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGTTA 
TGGCACGATG GAGTGTGCCC ATCAGTATCA AATCCTTTAG AAGTTTACCT CATTCCCACA 
CCACCTGAAA ATATAACATT TGAAGACCCG TCATTAGATG TGATCCTTCT TTTAAGAGTT 
TTACATGCTA TCAGTCGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAAATT 
ATTCCAACTA GTGAATTTAT TAACAGTAAG TTAACAGCAA AAGCAAATAG GCAACTTCAA 
GATCCTTTAG TAATCATGAC AGGAAACATC CCAACATGGC TTACTGAGCT AGGAAAAACC 
TGCCCATTTT TCTTTCCTTT TGATACCCGG CAAATGCTTT TTTATGTAAC TGCATTTGAT 
CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AAATCAACCA GTCTGATTCT 
CAAGATAGCA GAGTTGCACC TAGATTGGAT AGAAAAAAAC GTACTGTGAA CCGAGAGGAG 
CTGCTGAAAC AGGCGGAGTC TGTGATGCAG GACCTCGGCA GCTCACGGGC CATGTTAGAA 
ATCCAGTATG AAAATGAGGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCGCTT 
GTATCTCAGG AACTACAGAG AGCTGACTTG GGTCTTTGGA GAGGTGAAGA AGTAACTCTT 
AGCAATCCAA AAGGGAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 
GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA GCTCATATCG CAAAGGTTAA GATGAAGTTT 
CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTTGGCTTAC CCTTTTATAA ATGGATGCTA CGGCAAGAAA CTTCACTGAC ATCACACGAT 
TTGTTTGACA TCGACCCAGT TGTAGCCAGA TCAGTTTATC ACCTAGAAGA CATTGTCAGA 
CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTACA GTATGCATTA 
GAAACCTTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GACTGGATTT CACTCTGCCA 
GGGTTTCCCA ATATCGAACT GAAGAAAGGA GGGAAGGATA TACCAGTCAC TATCCACAAT 
TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCTAGGCAA 
TTTGATTCGT TCAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT TCAGTACTTC 
TACCCGGAGG AACTGGATCA GCTCCTTTGT GGCAGTAAAG CAGACACTTG GGATGCAAAG 
ACACTGATGG AATGCTGTAG GCCTGATCAT GGTTATACTC ATGACAGTCG GGCTGTGAAG 
TTTTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTATT TCTCCAGTTT 
GTGACTGGTA GCCCAAGATT GCCTGTTGGA GGATTCCGGA GTTTGAATCC ACCTTTGACA 
ATTGTCCGAA AGACGTTTGA ATCAACAGAA AACCCAGATG ACTTCTTGCC CTCTGTAATG 
ACTTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCA TTGAGATAAT GCGTGAAAAA 
CTGTTGATAG CAGCAAGAGA AGGGCAGCAG TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860772.1
CDS	204..6311
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005283.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005283.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 
ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 
GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG CTGCTCAGTT CCTAAATTCA 
GGAAATGTAA GACACAGGCT GCAGTTTTAT ATTGGAGAAC ATTTGCTGCC GTATAACATG 
ACTGTGTATC AGGCAGTACG GCAGTTTAGT ATACAGGCTG AAGATGAAAG AGAATCCACA 
GATGATGAGA GCAATCCTCT AGGCAGAGCT GGTATTTGGA CAAAGACTCA TACAATATGG 
TATAAACCTG TGAGAGAGGA TGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 
AGAGCCCAAA CAGCTCCAAC GAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGTTA 
TGGCACGATG GAGTGTGCCC ATCAGTATCA AATCCTTTAG AAGTTTACCT CATTCCCACA 
CCACCTGAAA ATATAACATT TGAAGACCCG TCATTAGATG TGATCCTTCT TTTAAGAGTT 
TTACATGCTA TCAGTCGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAAATT 
ATTCCAACTA GTGAATTTAT TAACAGTAAG TTAACAGCAA AAGCAAATAG GCAACTTCAA 
GATCCTTTAG TAATCATGAC AGGAAACATC CCAACATGGC TTACTGAGCT AGGAAAAACC 
TGCCCATTTT TCTTTCCTTT TGATACCCGG CAAATGCTTT TTTATGTAAC TGCATTTGAT 
CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AAATCAACCA GTCTGATTCT 
CAAGATAGCA GAGTTGCACC TAGATTGGAT AGAAAAAAAC GTACTGTGAA CCGAGAGGAG 
CTGCTGAAAC AGGCGGAGTC TGTGATGCAG GACCTCGGCA GCTCACGGGC CATGTTAGAA 
ATCCAGTATG AAAATGAGGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCGCTT 
GTATCTCAGG AACTACAGAG AGCTGACTTG GGTCTTTGGA GAGGTGAAGA AGTAACTCTT 
AGCAATCCAA AAGGGAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 
GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA GCTCATATCG CAAAGGTTAA GATGAAGTTT 
CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTTGGCTTAC CCTTTTATAA ATGGATGCTA CGGCAAGAAA CTTCACTGAC ATCACACGAT 
TTGTTTGACA TCGACCCAGT TGTAGCCAGA TCAGTTTATC ACCTAGAAGA CATTGTCAGA 
CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTACA GTATGCATTA 
GAAACCTTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GACTGGATTT CACTCTGCCA 
GGGTTTCCCA ATATCGAACT GAAGAAAGGA GGGAAGGATA TACCAGTCAC TATCCACAAT 
TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCTAGGCAA 
TTTGATTCGT TCAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT TCAGTACTTC 
TACCCGGAGG AACTGGATCA GCTCCTTTGT GGCAGTAAAG CAGACACTTG GGATGCAAAG 
ACACTGATGG AATGCTGTAG GCCTGATCAT GGTTATACTC ATGACAGTCG GGCTGTGAAG 
TTTTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTATT TCTCCAGTTT 
GTGACTGGTA GCCCAAGATT GCCTGTTGGA GGATTCCGGA GTTTGAATCC ACCTTTGACA 
ATTGTCCGAA AGACGTTTGA ATCAACAGAA AACCCAGATG ACTTCTTGCC CTCTGTAATG 
ACTTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCA TTGAGATAAT GCGTGAAAAA 
CTGTTGATAG CAGCAAGAGA AGGGCAGCAG TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005283.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860772.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

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CloneID	OHu92731
Clone ID Related Accession (Same CDS sequence)	XM_017005283.1 , XM_017005283.2
Accession Version	XM_017005283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6108bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 
ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 
GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG CTGCTCAGTT CCTAAATTCA 
GGAAATGTAA GACACAGGCT GCAGTTTTAT ATTGGAGAAC ATTTGCTGCC GTATAACATG 
ACTGTGTATC AGGCAGTACG GCAGTTTAGT ATACAGGCTG AAGATGAAAG AGAATCCACA 
GATGATGAGA GCAATCCTCT AGGCAGAGCT GGTATTTGGA CAAAGACTCA TACAATATGG 
TATAAACCTG TGAGAGAGGA TGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 
AGAGCCCAAA CAGCTCCAAC GAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGTTA 
TGGCACGATG GAGTGTGCCC ATCAGTATCA AATCCTTTAG AAGTTTACCT CATTCCCACA 
CCACCTGAAA ATATAACATT TGAAGACCCG TCATTAGATG TGATCCTTCT TTTAAGAGTT 
TTACATGCTA TCAGTCGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAAATT 
ATTCCAACTA GTGAATTTAT TAACAGTAAG TTAACAGCAA AAGCAAATAG GCAACTTCAA 
GATCCTTTAG TAATCATGAC AGGAAACATC CCAACATGGC TTACTGAGCT AGGAAAAACC 
TGCCCATTTT TCTTTCCTTT TGATACCCGG CAAATGCTTT TTTATGTAAC TGCATTTGAT 
CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AAATCAACCA GTCTGATTCT 
CAAGATAGCA GAGTTGCACC TAGATTGGAT AGAAAAAAAC GTACTGTGAA CCGAGAGGAG 
CTGCTGAAAC AGGCGGAGTC TGTGATGCAG GACCTCGGCA GCTCACGGGC CATGTTAGAA 
ATCCAGTATG AAAATGAGGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCGCTT 
GTATCTCAGG AACTACAGAG AGCTGACTTG GGTCTTTGGA GAGGTGAAGA AGTAACTCTT 
AGCAATCCAA AAGGGAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 
GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA GCTCATATCG CAAAGGTTAA GATGAAGTTT 
CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTTGGCTTAC CCTTTTATAA ATGGATGCTA CGGCAAGAAA CTTCACTGAC ATCACACGAT 
TTGTTTGACA TCGACCCAGT TGTAGCCAGA TCAGTTTATC ACCTAGAAGA CATTGTCAGA 
CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTACA GTATGCATTA 
GAAACCTTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GACTGGATTT CACTCTGCCA 
GGGTTTCCCA ATATCGAACT GAAGAAAGGA GGGAAGGATA TACCAGTCAC TATCCACAAT 
TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCTAGGCAA 
TTTGATTCGT TCAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT TCAGTACTTC 
TACCCGGAGG AACTGGATCA GCTCCTTTGT GGCAGTAAAG CAGACACTTG GGATGCAAAG 
ACACTGATGG AATGCTGTAG GCCTGATCAT GGTTATACTC ATGACAGTCG GGCTGTGAAG 
TTTTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTATT TCTCCAGTTT 
GTGACTGGTA GCCCAAGATT GCCTGTTGGA GGATTCCGGA GTTTGAATCC ACCTTTGACA 
ATTGTCCGAA AGACGTTTGA ATCAACAGAA AACCCAGATG ACTTCTTGCC CTCTGTAATG 
ACTTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCA TTGAGATAAT GCGTGAAAAA 
CTGTTGATAG CAGCAAGAGA AGGGCAGCAG TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860772.1
CDS	204..6311
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005283.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005283.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGAGG ATCACAGGCT 
TTAAAATTTT TCAACACACA TCAATTAAAA TGCCAGTTAC AAAGGCATCC AGACTGTGCA 
AATGTGAAGC AGTGGAAGGG TGGACCTGTC AAGATTGACC CTCTGGCTTT GGTACAAGCC 
ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT GGAAGAGTAA GAGAAGATGA TGAAGACAGC 
GATGACGATG GATCAGATGA GGAAATAGAT GAGTCTCTGG CTGCTCAGTT CCTAAATTCA 
GGAAATGTAA GACACAGGCT GCAGTTTTAT ATTGGAGAAC ATTTGCTGCC GTATAACATG 
ACTGTGTATC AGGCAGTACG GCAGTTTAGT ATACAGGCTG AAGATGAAAG AGAATCCACA 
GATGATGAGA GCAATCCTCT AGGCAGAGCT GGTATTTGGA CAAAGACTCA TACAATATGG 
TATAAACCTG TGAGAGAGGA TGAAGAAAGT AATAAAGATT GTGTTGGTGG TAAAAGAGGA 
AGAGCCCAAA CAGCTCCAAC GAAAACTTCC CCTAGAAATG CAAAAAAGCA TGATGAGTTA 
TGGCACGATG GAGTGTGCCC ATCAGTATCA AATCCTTTAG AAGTTTACCT CATTCCCACA 
CCACCTGAAA ATATAACATT TGAAGACCCG TCATTAGATG TGATCCTTCT TTTAAGAGTT 
TTACATGCTA TCAGTCGATA CTGGTATTAC TTGTATGATA ATGCAATGTG CAAGGAAATT 
ATTCCAACTA GTGAATTTAT TAACAGTAAG TTAACAGCAA AAGCAAATAG GCAACTTCAA 
GATCCTTTAG TAATCATGAC AGGAAACATC CCAACATGGC TTACTGAGCT AGGAAAAACC 
TGCCCATTTT TCTTTCCTTT TGATACCCGG CAAATGCTTT TTTATGTAAC TGCATTTGAT 
CGGGACCGAG CAATGCAAAG ATTACTTGAT ACCAACCCAG AAATCAACCA GTCTGATTCT 
CAAGATAGCA GAGTTGCACC TAGATTGGAT AGAAAAAAAC GTACTGTGAA CCGAGAGGAG 
CTGCTGAAAC AGGCGGAGTC TGTGATGCAG GACCTCGGCA GCTCACGGGC CATGTTAGAA 
ATCCAGTATG AAAATGAGGT TGGTACAGGT CTTGGGCCTA CACTGGAGTT TTATGCGCTT 
GTATCTCAGG AACTACAGAG AGCTGACTTG GGTCTTTGGA GAGGTGAAGA AGTAACTCTT 
AGCAATCCAA AAGGGAGCCA AGAAGGGACC AAGTATATTC AAAACCTCCA GGGCCTGTTT 
GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA GCTCATATCG CAAAGGTTAA GATGAAGTTT 
CGCTTCTTAG GAAAATTAAT GGCCAAGGCT ATCATGGATT TCAGATTGGT GGACCTTCCC 
CTTGGCTTAC CCTTTTATAA ATGGATGCTA CGGCAAGAAA CTTCACTGAC ATCACACGAT 
TTGTTTGACA TCGACCCAGT TGTAGCCAGA TCAGTTTATC ACCTAGAAGA CATTGTCAGA 
CAGAAGAAAA GACTTGAACA AGATAAATCC CAGACCAAAG AGAGTCTACA GTATGCATTA 
GAAACCTTGA CTATGAATGG CTGCTCAGTT GAAGATCTAG GACTGGATTT CACTCTGCCA 
GGGTTTCCCA ATATCGAACT GAAGAAAGGA GGGAAGGATA TACCAGTCAC TATCCACAAT 
TTAGAGGAGT ATCTAAGACT GGTTATATTC TGGGCACTAA ATGAAGGCGT TTCTAGGCAA 
TTTGATTCGT TCAGAGATGG ATTTGAATCA GTCTTCCCAC TCAGTCATCT TCAGTACTTC 
TACCCGGAGG AACTGGATCA GCTCCTTTGT GGCAGTAAAG CAGACACTTG GGATGCAAAG 
ACACTGATGG AATGCTGTAG GCCTGATCAT GGTTATACTC ATGACAGTCG GGCTGTGAAG 
TTTTTGTTTG AGATTCTCAG TAGTTTTGAT AATGAGCAGC AGAGGTTATT TCTCCAGTTT 
GTGACTGGTA GCCCAAGATT GCCTGTTGGA GGATTCCGGA GTTTGAATCC ACCTTTGACA 
ATTGTCCGAA AGACGTTTGA ATCAACAGAA AACCCAGATG ACTTCTTGCC CTCTGTAATG 
ACTTGTGTGA ACTATCTTAA GTTGCCGGAC TATTCAAGCA TTGAGATAAT GCGTGAAAAA 
CTGTTGATAG CAGCAAGAGA AGGGCAGCAG TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005289.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860778.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X30, mRNA.

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CloneID	OHu92732
Clone ID Related Accession (Same CDS sequence)	XM_017005289.1 , XM_017005289.2
Accession Version	XM_017005289.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TACAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860778.1
CDS	204..6242
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005289.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X30, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005289.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TACAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005289.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860778.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.

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CloneID	OHu92732
Clone ID Related Accession (Same CDS sequence)	XM_017005289.1 , XM_017005289.2
Accession Version	XM_017005289.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005289.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TACAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860778.1
CDS	204..6242
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005289.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005289.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TACAAATCAA TGAGGACACG GGAACAGCAC GTGCCATTCA GAGAAAACCT 
AACCCGTTAG CCAATAGTAA CACTAGTGGA TATTCAGAGT CAAAGAAGGA TGATGCTCGA 
GCACAGCTTA TGAAAGAGGA TCCGGAACTG GCTAAGTCTT TTATTAAGAC ATTATTTGGT 
GTTCTTTATG AAGTGTATAG TTCCTCAGCA GGACCTGCGG TCAGACATAA GTGCCTTAGA 
GCAATTCTTA GGATAATTTA TTTTGCGGAT GCTGAACTTC TGAAGGATGT TCTGAAAAAT 
CATGCTGTTT CAAGTCACAT TGCTTCCATG CTGTCAAGCC AAGACCTGAA GATAGTAGTG 
GGAGCACTTC AGATGGCAGA AATTTTAATG CAGAAGTTAC CTGATATTTT TAGTGTTTAC 
TTCAGAAGAG AAGGTGTAAT GCATCAAGTA AAACACTTAG CAGAATCAGA GTCTTTGTTG 
ACAAGTCCAC CAAAGGCATG TACGAATGGA TCGGGATCCA TGGGATCCAC AACTTCAGTC 
AGCAGTGGGA CAGCCACAGC TGCCACTCAT GCTGCAGCTG ACTTGGGATC ACCCAGCTTG 
CAGCACAGCA GGGATGATTC TTTAGATCTC AGCCCTCAAG GTCGATTAAG TGATGTTCTA 
AAGAGAAAAC GACTGCCAAA ACGAGGGCCA AGAAGGCCAA AGTACTCACC TCCAAGAGAT 
GATGACAAAG TAGACAATCA AGCTAAAAGC CCCACCACTA CTCAGTCACC TAAATCTTCT 
TTCCTGGCAA GCTTGAATCC AAAAACATGG GGAAGGTTAA GTACACAGTC CAACAGCAAC 
AACATTGAGC CAGCACGGAC TGCGGGAGGT AGTGGCCTTG CCAGGGCTGC CTCAAAGGAT 
ACCATCTCCA ATAATAGAGA AAAAATTAAA GGTTGGATTA AGGAGCAGGC ACATAAATTT 
GTAGAACGTT ATTTCAGTTC TGAGAATATG GATGGAAGCA ACCCTGCATT GAATGTCCTT 
CAGAGACTTT GTGCTGCAAC CGAACAACTC AACCTCCAGG TGGATGGTGG AGCTGAGTGC 
CTTGTAGAAA TCCGTAGCAT AGTCTCAGAG TCAGATGTTT CATCATTTGA AATCCAACAT 
AGTGGATTTG TGAAGCAGCT GTTGCTTTAT TTGACATCTA AAAGTGAAAA GGATGCTGTG 
AGCAGAGAGA TCAGATTAAA GCGATTTCTT CATGTATTTT TTTCTTCTCC ACTTCCTGGA 
GAAGAGCCCA TTGGAAGAGT GGAACCAGTG GGTAATGCAC CTTTGTTGGC ATTAGTTCAC 
AAGATGAACA ACTGCCTCAG CCAGATGGAA CAATTTCCAG TCAAAGTACA TGATTTCCCT 
AGTGGAAATG GGACAGGAGG CAGCTTTTCT CTCAACAGAG GATCACAGGC TTTAAAATTT 
TTCAACACAC ATCAATTAAA ATGCCAGTTA CAAAGGCATC CAGACTGTGC AAATGTGAAG 
CAGTGGAAGG GTGGACCTGT CAAGATTGAC CCTCTGGCTT TGGTACAAGC CATCGAGAGA 
TACCTTGTAG TTAGAGGGTA TGGAAGAGTA AGAGAAGATG ATGAAGACAG CGATGACGAT 
GGATCAGATG AGGAAATAGA TGAGTCTCTG GCTGCTCAGT TCCTAAATTC AGGAAATGTA 
AGACACAGGC TGCAGTTTTA TATTGGAGAA CATTTGCTGC CGTATAACAT GACTGTGTAT 
CAGGCAGTAC GGCAGTTTAG TATACAGGCT GAAGATGAAA GAGAATCCAC AGATGATGAG 
AGCAATCCTC TAGGCAGAGC TGGTATTTGG ACAAAGACTC ATACAATATG GTATAAACCT 
GTGAGAGAGG ATGAAGAAAG TAATAAAGAT TGTGTTGGTG GTAAAAGAGG AAGAGCCCAA 
ACAGCTCCAA CGAAAACTTC CCCTAGAAAT GCAAAAAAGC ATGATGAGTT ATGGCACGAT 
GGAGTGTGCC CATCAGTATC AAATCCTTTA GAAGTTTACC TCATTCCCAC ACCACCTGAA 
AATATAACAT TTGAAGACCC GTCATTAGAT GTGATCCTTC TTTTAAGAGT TTTACATGCT 
ATCAGTCGAT ACTGGTATTA CTTGTATGAT AATGCAATGT GCAAGGAAAT TATTCCAACT 
AGTGAATTTA TTAACAGTAA GTTAACAGCA AAAGCAAATA GGCAACTTCA AGATCCTTTA 
GTAATCATGA CAGGAAACAT CCCAACATGG CTTACTGAGC TAGGAAAAAC CTGCCCATTT 
TTCTTTCCTT TTGATACCCG GCAAATGCTT TTTTATGTAA CTGCATTTGA TCGGGACCGA 
GCAATGCAAA GATTACTTGA TACCAACCCA GAAATCAACC AGTCTGATTC TCAAGATAGC 
AGAGTTGCAC CTAGATTGGA TAGAAAAAAA CGTACTGTGA ACCGAGAGGA GCTGCTGAAA 
CAGGCGGAGT CTGTGATGCA GGACCTCGGC AGCTCACGGG CCATGTTAGA AATCCAGTAT 
GAAAATGAGG TTGGTACAGG TCTTGGGCCT ACACTGGAGT TTTATGCGCT TGTATCTCAG 
GAACTACAGA GAGCTGACTT GGGTCTTTGG AGAGGTGAAG AAGTAACTCT TAGCAATCCA 
AAAGGGAGCC AAGAAGGGAC CAAGTATATT CAAAACCTCC AGGGCCTGTT TGCGCTTCCC 
TTTGGTAGGA CAGCAAAGCC AGCTCATATC GCAAAGGTTA AGATGAAGTT TCGCTTCTTA 
GGAAAATTAA TGGCCAAGGC TATCATGGAT TTCAGATTGG TGGACCTTCC CCTTGGCTTA 
CCCTTTTATA AATGGATGCT ACGGCAAGAA ACTTCACTGA CATCACACGA TTTGTTTGAC 
ATCGACCCAG TTGTAGCCAG ATCAGTTTAT CACCTAGAAG ACATTGTCAG ACAGAAGAAA 
AGACTTGAAC AAGATAAATC CCAGACCAAA GAGAGTCTAC AGTATGCATT AGAAACCTTG 
ACTATGAATG GCTGCTCAGT TGAAGATCTA GGACTGGATT TCACTCTGCC AGGGTTTCCC 
AATATCGAAC TGAAGAAAGG AGGGAAGGAT ATACCAGTCA CTATCCACAA TTTAGAGGAG 
TATCTAAGAC TGGTTATATT CTGGGCACTA AATGAAGGCG TTTCTAGGCA ATTTGATTCG 
TTCAGAGATG GATTTGAATC AGTCTTCCCA CTCAGTCATC TTCAGTACTT CTACCCGGAG 
GAACTGGATC AGCTCCTTTG TGGCAGTAAA GCAGACACTT GGGATGCAAA GACACTGATG 
GAATGCTGTA GGCCTGATCA TGGTTATACT CATGACAGTC GGGCTGTGAA GTTTTTGTTT 
GAGATTCTCA GTAGTTTTGA TAATGAGCAG CAGAGGTTAT TTCTCCAGTT TGTGACTGGT 
AGCCCAAGAT TGCCTGTTGG AGGATTCCGG AGTTTGAATC CACCTTTGAC AATTGTCCGA 
AAGACGTTTG AATCAACAGA AAACCCAGAT GACTTCTTGC CCTCTGTAAT GACTTGTGTG 
AACTATCTTA AGTTGCCGGA CTATTCAAGC ATTGAGATAA TGCGTGAAAA ACTGTTGATA 
GCAGCAAGAG AAGGGCAGCA GTCGTTCCAT CTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005290.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860779.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.

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CloneID	OHu92733
Clone ID Related Accession (Same CDS sequence)	XM_017005290.2 , XM_017005290.1
Accession Version	XM_017005290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017005290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCGTTAGCCA ATAGTAACAC TAGTGGATAT TCAGAGTCAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCGGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA 
GCACTTCAGA TGGCAGAAAT TTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCACCAA AGGCATGTAC GAATGGATCG GGATCCATGG GATCCACAAC TTCAGTCAGC 
AGTGGGACAG CCACAGCTGC CACTCATGCT GCAGCTGACT TGGGATCACC CAGCTTGCAG 
CACAGCAGGG ATGATTCTTT AGATCTCAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAGT ACTCACCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCTTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGTA CACAGTCCAA CAGCAACAAC 
ATTGAGCCAG CACGGACTGC GGGAGGTAGT GGCCTTGCCA GGGCTGCCTC AAAGGATACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAGG AGCAGGCACA TAAATTTGTA 
GAACGTTATT TCAGTTCTGA GAATATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACCGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCAT CATTTGAAAT CCAACATAGT 
GGATTTGTGA AGCAGCTGTT GCTTTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGCG ATTTCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG GAAGAGTGGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAG 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGAAATGGGA CAGGAGGCAG CTTTTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AATTAAAATG CCAGTTACAA AGGCATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCT CTGGCTTTGG TACAAGCCAT CGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGATG AAGACAGCGA TGACGATGGA 
TCAGATGAGG AAATAGATGA GTCTCTGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860779.1
CDS	204..6239
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005290.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005290.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCGTTAGCCA ATAGTAACAC TAGTGGATAT TCAGAGTCAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCGGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA 
GCACTTCAGA TGGCAGAAAT TTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCACCAA AGGCATGTAC GAATGGATCG GGATCCATGG GATCCACAAC TTCAGTCAGC 
AGTGGGACAG CCACAGCTGC CACTCATGCT GCAGCTGACT TGGGATCACC CAGCTTGCAG 
CACAGCAGGG ATGATTCTTT AGATCTCAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAGT ACTCACCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCTTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGTA CACAGTCCAA CAGCAACAAC 
ATTGAGCCAG CACGGACTGC GGGAGGTAGT GGCCTTGCCA GGGCTGCCTC AAAGGATACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAGG AGCAGGCACA TAAATTTGTA 
GAACGTTATT TCAGTTCTGA GAATATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACCGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCAT CATTTGAAAT CCAACATAGT 
GGATTTGTGA AGCAGCTGTT GCTTTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGCG ATTTCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG GAAGAGTGGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAG 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGAAATGGGA CAGGAGGCAG CTTTTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AATTAAAATG CCAGTTACAA AGGCATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCT CTGGCTTTGG TACAAGCCAT CGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGATG AAGACAGCGA TGACGATGGA 
TCAGATGAGG AAATAGATGA GTCTCTGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005290.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860779.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X31, mRNA.

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CloneID	OHu92733
Clone ID Related Accession (Same CDS sequence)	XM_017005290.2 , XM_017005290.1
Accession Version	XM_017005290.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCGTTAGCCA ATAGTAACAC TAGTGGATAT TCAGAGTCAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCGGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA 
GCACTTCAGA TGGCAGAAAT TTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCACCAA AGGCATGTAC GAATGGATCG GGATCCATGG GATCCACAAC TTCAGTCAGC 
AGTGGGACAG CCACAGCTGC CACTCATGCT GCAGCTGACT TGGGATCACC CAGCTTGCAG 
CACAGCAGGG ATGATTCTTT AGATCTCAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAGT ACTCACCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCTTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGTA CACAGTCCAA CAGCAACAAC 
ATTGAGCCAG CACGGACTGC GGGAGGTAGT GGCCTTGCCA GGGCTGCCTC AAAGGATACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAGG AGCAGGCACA TAAATTTGTA 
GAACGTTATT TCAGTTCTGA GAATATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACCGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCAT CATTTGAAAT CCAACATAGT 
GGATTTGTGA AGCAGCTGTT GCTTTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGCG ATTTCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG GAAGAGTGGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAG 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGAAATGGGA CAGGAGGCAG CTTTTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AATTAAAATG CCAGTTACAA AGGCATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCT CTGGCTTTGG TACAAGCCAT CGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGATG AAGACAGCGA TGACGATGGA 
TCAGATGAGG AAATAGATGA GTCTCTGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860779.1
CDS	204..6239
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005290.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X31, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005290.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGC AAATCAATGA GGACACGGGA ACAGCACGTG CCATTCAGAG AAAACCTAAC 
CCGTTAGCCA ATAGTAACAC TAGTGGATAT TCAGAGTCAA AGAAGGATGA TGCTCGAGCA 
CAGCTTATGA AAGAGGATCC GGAACTGGCT AAGTCTTTTA TTAAGACATT ATTTGGTGTT 
CTTTATGAAG TGTATAGTTC CTCAGCAGGA CCTGCGGTCA GACATAAGTG CCTTAGAGCA 
ATTCTTAGGA TAATTTATTT TGCGGATGCT GAACTTCTGA AGGATGTTCT GAAAAATCAT 
GCTGTTTCAA GTCACATTGC TTCCATGCTG TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA 
GCACTTCAGA TGGCAGAAAT TTTAATGCAG AAGTTACCTG ATATTTTTAG TGTTTACTTC 
AGAAGAGAAG GTGTAATGCA TCAAGTAAAA CACTTAGCAG AATCAGAGTC TTTGTTGACA 
AGTCCACCAA AGGCATGTAC GAATGGATCG GGATCCATGG GATCCACAAC TTCAGTCAGC 
AGTGGGACAG CCACAGCTGC CACTCATGCT GCAGCTGACT TGGGATCACC CAGCTTGCAG 
CACAGCAGGG ATGATTCTTT AGATCTCAGC CCTCAAGGTC GATTAAGTGA TGTTCTAAAG 
AGAAAACGAC TGCCAAAACG AGGGCCAAGA AGGCCAAAGT ACTCACCTCC AAGAGATGAT 
GACAAAGTAG ACAATCAAGC TAAAAGCCCC ACCACTACTC AGTCACCTAA ATCTTCTTTC 
CTGGCAAGCT TGAATCCAAA AACATGGGGA AGGTTAAGTA CACAGTCCAA CAGCAACAAC 
ATTGAGCCAG CACGGACTGC GGGAGGTAGT GGCCTTGCCA GGGCTGCCTC AAAGGATACC 
ATCTCCAATA ATAGAGAAAA AATTAAAGGT TGGATTAAGG AGCAGGCACA TAAATTTGTA 
GAACGTTATT TCAGTTCTGA GAATATGGAT GGAAGCAACC CTGCATTGAA TGTCCTTCAG 
AGACTTTGTG CTGCAACCGA ACAACTCAAC CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT 
GTAGAAATCC GTAGCATAGT CTCAGAGTCA GATGTTTCAT CATTTGAAAT CCAACATAGT 
GGATTTGTGA AGCAGCTGTT GCTTTATTTG ACATCTAAAA GTGAAAAGGA TGCTGTGAGC 
AGAGAGATCA GATTAAAGCG ATTTCTTCAT GTATTTTTTT CTTCTCCACT TCCTGGAGAA 
GAGCCCATTG GAAGAGTGGA ACCAGTGGGT AATGCACCTT TGTTGGCATT AGTTCACAAG 
ATGAACAACT GCCTCAGCCA GATGGAACAA TTTCCAGTCA AAGTACATGA TTTCCCTAGT 
GGAAATGGGA CAGGAGGCAG CTTTTCTCTC AACAGAGGAT CACAGGCTTT AAAATTTTTC 
AACACACATC AATTAAAATG CCAGTTACAA AGGCATCCAG ACTGTGCAAA TGTGAAGCAG 
TGGAAGGGTG GACCTGTCAA GATTGACCCT CTGGCTTTGG TACAAGCCAT CGAGAGATAC 
CTTGTAGTTA GAGGGTATGG AAGAGTAAGA GAAGATGATG AAGACAGCGA TGACGATGGA 
TCAGATGAGG AAATAGATGA GTCTCTGGCT GCTCAGTTCC TAAATTCAGG AAATGTAAGA 
CACAGGCTGC AGTTTTATAT TGGAGAACAT TTGCTGCCGT ATAACATGAC TGTGTATCAG 
GCAGTACGGC AGTTTAGTAT ACAGGCTGAA GATGAAAGAG AATCCACAGA TGATGAGAGC 
AATCCTCTAG GCAGAGCTGG TATTTGGACA AAGACTCATA CAATATGGTA TAAACCTGTG 
AGAGAGGATG AAGAAAGTAA TAAAGATTGT GTTGGTGGTA AAAGAGGAAG AGCCCAAACA 
GCTCCAACGA AAACTTCCCC TAGAAATGCA AAAAAGCATG ATGAGTTATG GCACGATGGA 
GTGTGCCCAT CAGTATCAAA TCCTTTAGAA GTTTACCTCA TTCCCACACC ACCTGAAAAT 
ATAACATTTG AAGACCCGTC ATTAGATGTG ATCCTTCTTT TAAGAGTTTT ACATGCTATC 
AGTCGATACT GGTATTACTT GTATGATAAT GCAATGTGCA AGGAAATTAT TCCAACTAGT 
GAATTTATTA ACAGTAAGTT AACAGCAAAA GCAAATAGGC AACTTCAAGA TCCTTTAGTA 
ATCATGACAG GAAACATCCC AACATGGCTT ACTGAGCTAG GAAAAACCTG CCCATTTTTC 
TTTCCTTTTG ATACCCGGCA AATGCTTTTT TATGTAACTG CATTTGATCG GGACCGAGCA 
ATGCAAAGAT TACTTGATAC CAACCCAGAA ATCAACCAGT CTGATTCTCA AGATAGCAGA 
GTTGCACCTA GATTGGATAG AAAAAAACGT ACTGTGAACC GAGAGGAGCT GCTGAAACAG 
GCGGAGTCTG TGATGCAGGA CCTCGGCAGC TCACGGGCCA TGTTAGAAAT CCAGTATGAA 
AATGAGGTTG GTACAGGTCT TGGGCCTACA CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA 
CTACAGAGAG CTGACTTGGG TCTTTGGAGA GGTGAAGAAG TAACTCTTAG CAATCCAAAA 
GGGAGCCAAG AAGGGACCAA GTATATTCAA AACCTCCAGG GCCTGTTTGC GCTTCCCTTT 
GGTAGGACAG CAAAGCCAGC TCATATCGCA AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA 
AAATTAATGG CCAAGGCTAT CATGGATTTC AGATTGGTGG ACCTTCCCCT TGGCTTACCC 
TTTTATAAAT GGATGCTACG GCAAGAAACT TCACTGACAT CACACGATTT GTTTGACATC 
GACCCAGTTG TAGCCAGATC AGTTTATCAC CTAGAAGACA TTGTCAGACA GAAGAAAAGA 
CTTGAACAAG ATAAATCCCA GACCAAAGAG AGTCTACAGT ATGCATTAGA AACCTTGACT 
ATGAATGGCT GCTCAGTTGA AGATCTAGGA CTGGATTTCA CTCTGCCAGG GTTTCCCAAT 
ATCGAACTGA AGAAAGGAGG GAAGGATATA CCAGTCACTA TCCACAATTT AGAGGAGTAT 
CTAAGACTGG TTATATTCTG GGCACTAAAT GAAGGCGTTT CTAGGCAATT TGATTCGTTC 
AGAGATGGAT TTGAATCAGT CTTCCCACTC AGTCATCTTC AGTACTTCTA CCCGGAGGAA 
CTGGATCAGC TCCTTTGTGG CAGTAAAGCA GACACTTGGG ATGCAAAGAC ACTGATGGAA 
TGCTGTAGGC CTGATCATGG TTATACTCAT GACAGTCGGG CTGTGAAGTT TTTGTTTGAG 
ATTCTCAGTA GTTTTGATAA TGAGCAGCAG AGGTTATTTC TCCAGTTTGT GACTGGTAGC 
CCAAGATTGC CTGTTGGAGG ATTCCGGAGT TTGAATCCAC CTTTGACAAT TGTCCGAAAG 
ACGTTTGAAT CAACAGAAAA CCCAGATGAC TTCTTGCCCT CTGTAATGAC TTGTGTGAAC 
TATCTTAAGT TGCCGGACTA TTCAAGCATT GAGATAATGC GTGAAAAACT GTTGATAGCA 
GCAAGAGAAG GGCAGCAGTC GTTCCATCTT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348315.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335244.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 5, mRNA.

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CloneID	OHu08006
Clone ID Related Accession (Same CDS sequence)	NM_001348315.1 , NM_001284214.2 , NM_001284214.1 , NM_001348315.2
Accession Version	NM_001348315.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 28, 2017 this sequence version replaced XM_017005281.1. Transcript Variant: This variant (5), as well as variant 1, encodes isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.181601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335244.1
CDS	168..6290
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348315.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348315.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001284214.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271143.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 1, mRNA.

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CloneID	OHu08006
Clone ID Related Accession (Same CDS sequence)	NM_001348315.1 , NM_001284214.2 , NM_001284214.1 , NM_001348315.2
Accession Version	NM_001284214.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240964.1, D28476.1, BC114556.1, AC093384.4 and BM677880.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001284214.1. Transcript Variant: This variant (1), as well as variant 5, encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.264252.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271143.1
CDS	180..6302
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001284214.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284214.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001284214.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271143.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 1, mRNA.

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CloneID	OHu08006
Clone ID Related Accession (Same CDS sequence)	NM_001348315.1 , NM_001284214.2 , NM_001284214.1 , NM_001348315.2
Accession Version	NM_001284214.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB240964.1, D28476.1, BC114556.1, AC093384.4 and BM677880.1. This sequence is a reference standard in the RefSeqGene project. On Sep 26, 2013 this sequence version replaced XM_005246959.1. Transcript Variant: This variant (1), as well as variant 5, encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.264252.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271143.1
CDS	180..6302
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001284214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284214.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348315.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335244.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 5, mRNA.

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CloneID	OHu08006
Clone ID Related Accession (Same CDS sequence)	NM_001348315.1 , NM_001284214.2 , NM_001284214.1 , NM_001348315.2
Accession Version	NM_001348315.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348315.1. Transcript Variant: This variant (5), as well as variant 1, encodes isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.181601.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335244.1
CDS	569..6691
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348315.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348315.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGCAAA TCAATGAGGA CACGGGAACA 
GCACGTGCCA TTCAGAGAAA ACCTAACCCG TTAGCCAATA GTAACACTAG TGGATATTCA 
GAGTCAAAGA AGGATGATGC TCGAGCACAG CTTATGAAAG AGGATCCGGA ACTGGCTAAG 
TCTTTTATTA AGACATTATT TGGTGTTCTT TATGAAGTGT ATAGTTCCTC AGCAGGACCT 
GCGGTCAGAC ATAAGTGCCT TAGAGCAATT CTTAGGATAA TTTATTTTGC GGATGCTGAA 
CTTCTGAAGG ATGTTCTGAA AAATCATGCT GTTTCAAGTC ACATTGCTTC CATGCTGTCA 
AGCCAAGACC TGAAGATAGT AGTGGGAGCA CTTCAGATGG CAGAAATTTT AATGCAGAAG 
TTACCTGATA TTTTTAGTGT TTACTTCAGA AGAGAAGGTG TAATGCATCA AGTAAAACAC 
TTAGCAGAAT CAGAGTCTTT GTTGACAAGT CCACCAAAGG CATGTACGAA TGGATCGGGA 
TCCATGGGAT CCACAACTTC AGTCAGCAGT GGGACAGCCA CAGCTGCCAC TCATGCTGCA 
GCTGACTTGG GATCACCCAG CTTGCAGCAC AGCAGGGATG ATTCTTTAGA TCTCAGCCCT 
CAAGGTCGAT TAAGTGATGT TCTAAAGAGA AAACGACTGC CAAAACGAGG GCCAAGAAGG 
CCAAAGTACT CACCTCCAAG AGATGATGAC AAAGTAGACA ATCAAGCTAA AAGCCCCACC 
ACTACTCAGT CACCTAAATC TTCTTTCCTG GCAAGCTTGA ATCCAAAAAC ATGGGGAAGG 
TTAAGTACAC AGTCCAACAG CAACAACATT GAGCCAGCAC GGACTGCGGG AGGTAGTGGC 
CTTGCCAGGG CTGCCTCAAA GGATACCATC TCCAATAATA GAGAAAAAAT TAAAGGTTGG 
ATTAAGGAGC AGGCACATAA ATTTGTAGAA CGTTATTTCA GTTCTGAGAA TATGGATGGA 
AGCAACCCTG CATTGAATGT CCTTCAGAGA CTTTGTGCTG CAACCGAACA ACTCAACCTC 
CAGGTGGATG GTGGAGCTGA GTGCCTTGTA GAAATCCGTA GCATAGTCTC AGAGTCAGAT 
GTTTCATCAT TTGAAATCCA ACATAGTGGA TTTGTGAAGC AGCTGTTGCT TTATTTGACA 
TCTAAAAGTG AAAAGGATGC TGTGAGCAGA GAGATCAGAT TAAAGCGATT TCTTCATGTA 
TTTTTTTCTT CTCCACTTCC TGGAGAAGAG CCCATTGGAA GAGTGGAACC AGTGGGTAAT 
GCACCTTTGT TGGCATTAGT TCACAAGATG AACAACTGCC TCAGCCAGAT GGAACAATTT 
CCAGTCAAAG TACATGATTT CCCTAGTGGA AATGGGACAG GAGGCAGCTT TTCTCTCAAC 
AGAGGATCAC AGGCTTTAAA ATTTTTCAAC ACACATCAAT TAAAATGCCA GTTACAAAGG 
CATCCAGACT GTGCAAATGT GAAGCAGTGG AAGGGTGGAC CTGTCAAGAT TGACCCTCTG 
GCTTTGGTAC AAGCCATCGA GAGATACCTT GTAGTTAGAG GGTATGGAAG AGTAAGAGAA 
GATGATGAAG ACAGCGATGA CGATGGATCA GATGAGGAAA TAGATGAGTC TCTGGCTGCT 
CAGTTCCTAA ATTCAGGAAA TGTAAGACAC AGGCTGCAGT TTTATATTGG AGAACATTTG 
CTGCCGTATA ACATGACTGT GTATCAGGCA GTACGGCAGT TTAGTATACA GGCTGAAGAT 
GAAAGAGAAT CCACAGATGA TGAGAGCAAT CCTCTAGGCA GAGCTGGTAT TTGGACAAAG 
ACTCATACAA TATGGTATAA ACCTGTGAGA GAGGATGAAG AAAGTAATAA AGATTGTGTT 
GGTGGTAAAA GAGGAAGAGC CCAAACAGCT CCAACGAAAA CTTCCCCTAG AAATGCAAAA 
AAGCATGATG AGTTATGGCA CGATGGAGTG TGCCCATCAG TATCAAATCC TTTAGAAGTT 
TACCTCATTC CCACACCACC TGAAAATATA ACATTTGAAG ACCCGTCATT AGATGTGATC 
CTTCTTTTAA GAGTTTTACA TGCTATCAGT CGATACTGGT ATTACTTGTA TGATAATGCA 
ATGTGCAAGG AAATTATTCC AACTAGTGAA TTTATTAACA GTAAGTTAAC AGCAAAAGCA 
AATAGGCAAC TTCAAGATCC TTTAGTAATC ATGACAGGAA ACATCCCAAC ATGGCTTACT 
GAGCTAGGAA AAACCTGCCC ATTTTTCTTT CCTTTTGATA CCCGGCAAAT GCTTTTTTAT 
GTAACTGCAT TTGATCGGGA CCGAGCAATG CAAAGATTAC TTGATACCAA CCCAGAAATC 
AACCAGTCTG ATTCTCAAGA TAGCAGAGTT GCACCTAGAT TGGATAGAAA AAAACGTACT 
GTGAACCGAG AGGAGCTGCT GAAACAGGCG GAGTCTGTGA TGCAGGACCT CGGCAGCTCA 
CGGGCCATGT TAGAAATCCA GTATGAAAAT GAGGTTGGTA CAGGTCTTGG GCCTACACTG 
GAGTTTTATG CGCTTGTATC TCAGGAACTA CAGAGAGCTG ACTTGGGTCT TTGGAGAGGT 
GAAGAAGTAA CTCTTAGCAA TCCAAAAGGG AGCCAAGAAG GGACCAAGTA TATTCAAAAC 
CTCCAGGGCC TGTTTGCGCT TCCCTTTGGT AGGACAGCAA AGCCAGCTCA TATCGCAAAG 
GTTAAGATGA AGTTTCGCTT CTTAGGAAAA TTAATGGCCA AGGCTATCAT GGATTTCAGA 
TTGGTGGACC TTCCCCTTGG CTTACCCTTT TATAAATGGA TGCTACGGCA AGAAACTTCA 
CTGACATCAC ACGATTTGTT TGACATCGAC CCAGTTGTAG CCAGATCAGT TTATCACCTA 
GAAGACATTG TCAGACAGAA GAAAAGACTT GAACAAGATA AATCCCAGAC CAAAGAGAGT 
CTACAGTATG CATTAGAAAC CTTGACTATG AATGGCTGCT CAGTTGAAGA TCTAGGACTG 
GATTTCACTC TGCCAGGGTT TCCCAATATC GAACTGAAGA AAGGAGGGAA GGATATACCA 
GTCACTATCC ACAATTTAGA GGAGTATCTA AGACTGGTTA TATTCTGGGC ACTAAATGAA 
GGCGTTTCTA GGCAATTTGA TTCGTTCAGA GATGGATTTG AATCAGTCTT CCCACTCAGT 
CATCTTCAGT ACTTCTACCC GGAGGAACTG GATCAGCTCC TTTGTGGCAG TAAAGCAGAC 
ACTTGGGATG CAAAGACACT GATGGAATGC TGTAGGCCTG ATCATGGTTA TACTCATGAC 
AGTCGGGCTG TGAAGTTTTT GTTTGAGATT CTCAGTAGTT TTGATAATGA GCAGCAGAGG 
TTATTTCTCC AGTTTGTGAC TGGTAGCCCA AGATTGCCTG TTGGAGGATT CCGGAGTTTG 
AATCCACCTT TGACAATTGT CCGAAAGACG TTTGAATCAA CAGAAAACCC AGATGACTTC 
TTGCCCTCTG TAATGACTTG TGTGAACTAT CTTAAGTTGC CGGACTATTC AAGCATTGAG 
ATAATGCGTG AAAAACTGTT GATAGCAGCA AGAGAAGGGC AGCAGTCGTT CCATCTTTCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348316.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335245.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 6, mRNA.

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CloneID	OHu08432
Clone ID Related Accession (Same CDS sequence)	NM_001348316.1 , NM_001284215.2 , NM_001348316.2
Accession Version	NM_001348316.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005286.1. Transcript Variant: This variant (6), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.236482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA 
TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT 
GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335245.1
CDS	166..6243
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348316.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348316.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA 
TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT 
GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001284215.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001271144.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 2, mRNA.

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CloneID	OHu08432
Clone ID Related Accession (Same CDS sequence)	NM_001348316.1 , NM_001284215.2 , NM_001348316.2
Accession Version	NM_001284215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On or before Jan 27, 2017 this sequence version replaced XM_005246962.4, NM_001284215.1. Transcript Variant: This variant (2), as well as variant 6, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.129209.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA 
TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT 
GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001271144.1
CDS	180..6257
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001284215.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001284215.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA 
TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT 
GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348316.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335245.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 6, mRNA.

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CloneID	OHu08432
Clone ID Related Accession (Same CDS sequence)	NM_001348316.1 , NM_001284215.2 , NM_001348316.2
Accession Version	NM_001348316.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348316.1. Transcript Variant: This variant (6), as well as variant 2, encodes isoform b. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.236482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA 
TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT 
GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335245.1
CDS	276..6353
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348316.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348316.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GCAGCCCATC AGGTCGGTGA GGATGAGATA 
AGCTTGTCCA CTCTGGGACG AGTTTATACT ATTGATTTTA ATTCTATGCA GCAAATCAAT 
GAGGACACGG GAACAGCACG TGCCATTCAG AGAAAACCTA ACCCGTTAGC CAATAGTAAC 
ACTAGTGGAT ATTCAGAGTC AAAGAAGGAT GATGCTCGAG CACAGCTTAT GAAAGAGGAT 
CCGGAACTGG CTAAGTCTTT TATTAAGACA TTATTTGGTG TTCTTTATGA AGTGTATAGT 
TCCTCAGCAG GACCTGCGGT CAGACATAAG TGCCTTAGAG CAATTCTTAG GATAATTTAT 
TTTGCGGATG CTGAACTTCT GAAGGATGTT CTGAAAAATC ATGCTGTTTC AAGTCACATT 
GCTTCCATGC TGTCAAGCCA AGACCTGAAG ATAGTAGTGG GAGCACTTCA GATGGCAGAA 
ATTTTAATGC AGAAGTTACC TGATATTTTT AGTGTTTACT TCAGAAGAGA AGGTGTAATG 
CATCAAGTAA AACACTTAGC AGAATCAGAG TCTTTGTTGA CAAGTCCACC AAAGGCATGT 
ACGAATGGAT CGGGATCCAT GGGATCCACA ACTTCAGTCA GCAGTGGGAC AGCCACAGCT 
GCCACTCATG CTGCAGCTGA CTTGGGATCA CCCAGCTTGC AGCACAGCAG GGATGATTCT 
TTAGATCTCA GCCCTCAAGG TCGATTAAGT GATGTTCTAA AGAGAAAACG ACTGCCAAAA 
CGAGGGCCAA GAAGGCCAAA GTACTCACCT CCAAGAGATG ATGACAAAGT AGACAATCAA 
GCTAAAAGCC CCACCACTAC TCAGTCACCT AAATCTTCTT TCCTGGCAAG CTTGAATCCA 
AAAACATGGG GAAGGTTAAG TACACAGTCC AACAGCAACA ACATTGAGCC AGCACGGACT 
GCGGGAGGTA GTGGCCTTGC CAGGGCTGCC TCAAAGGATA CCATCTCCAA TAATAGAGAA 
AAAATTAAAG GTTGGATTAA GGAGCAGGCA CATAAATTTG TAGAACGTTA TTTCAGTTCT 
GAGAATATGG ATGGAAGCAA CCCTGCATTG AATGTCCTTC AGAGACTTTG TGCTGCAACC 
GAACAACTCA ACCTCCAGGT GGATGGTGGA GCTGAGTGCC TTGTAGAAAT CCGTAGCATA 
GTCTCAGAGT CAGATGTTTC ATCATTTGAA ATCCAACATA GTGGATTTGT GAAGCAGCTG 
TTGCTTTATT TGACATCTAA AAGTGAAAAG GATGCTGTGA GCAGAGAGAT CAGATTAAAG 
CGATTTCTTC ATGTATTTTT TTCTTCTCCA CTTCCTGGAG AAGAGCCCAT TGGAAGAGTG 
GAACCAGTGG GTAATGCACC TTTGTTGGCA TTAGTTCACA AGATGAACAA CTGCCTCAGC 
CAGATGGAAC AATTTCCAGT CAAAGTACAT GATTTCCCTA GTGGAAATGG GACAGGAGGC 
AGCTTTTCTC TCAACAGAGG ATCACAGGCT TTAAAATTTT TCAACACACA TCAATTAAAA 
TGCCAGTTAC AAAGGCATCC AGACTGTGCA AATGTGAAGC AGTGGAAGGG TGGACCTGTC 
AAGATTGACC CTCTGGCTTT GGTACAAGCC ATCGAGAGAT ACCTTGTAGT TAGAGGGTAT 
GGAAGAGTAA GAGAAGATGA TGAAGACAGC GATGACGATG GATCAGATGA GGAAATAGAT 
GAGTCTCTGG CTGCTCAGTT CCTAAATTCA GGAAATGTAA GACACAGGCT GCAGTTTTAT 
ATTGGAGAAC ATTTGCTGCC GTATAACATG ACTGTGTATC AGGCAGTACG GCAGTTTAGT 
ATACAGGCTG AAGATGAAAG AGAATCCACA GATGATGAGA GCAATCCTCT AGGCAGAGCT 
GGTATTTGGA CAAAGACTCA TACAATATGG TATAAACCTG TGAGAGAGGA TGAAGAAAGT 
AATAAAGATT GTGTTGGTGG TAAAAGAGGA AGAGCCCAAA CAGCTCCAAC GAAAACTTCC 
CCTAGAAATG CAAAAAAGCA TGATGAGTTA TGGCACGATG GAGTGTGCCC ATCAGTATCA 
AATCCTTTAG AAGTTTACCT CATTCCCACA CCACCTGAAA ATATAACATT TGAAGACCCG 
TCATTAGATG TGATCCTTCT TTTAAGAGTT TTACATGCTA TCAGTCGATA CTGGTATTAC 
TTGTATGATA ATGCAATGTG CAAGGAAATT ATTCCAACTA GTGAATTTAT TAACAGTAAG 
TTAACAGCAA AAGCAAATAG GCAACTTCAA GATCCTTTAG TAATCATGAC AGGAAACATC 
CCAACATGGC TTACTGAGCT AGGAAAAACC TGCCCATTTT TCTTTCCTTT TGATACCCGG 
CAAATGCTTT TTTATGTAAC TGCATTTGAT CGGGACCGAG CAATGCAAAG ATTACTTGAT 
ACCAACCCAG AAATCAACCA GTCTGATTCT CAAGATAGCA GAGTTGCACC TAGATTGGAT 
AGAAAAAAAC GTACTGTGAA CCGAGAGGAG CTGCTGAAAC AGGCGGAGTC TGTGATGCAG 
GACCTCGGCA GCTCACGGGC CATGTTAGAA ATCCAGTATG AAAATGAGGT TGGTACAGGT 
CTTGGGCCTA CACTGGAGTT TTATGCGCTT GTATCTCAGG AACTACAGAG AGCTGACTTG 
GGTCTTTGGA GAGGTGAAGA AGTAACTCTT AGCAATCCAA AAGGGAGCCA AGAAGGGACC 
AAGTATATTC AAAACCTCCA GGGCCTGTTT GCGCTTCCCT TTGGTAGGAC AGCAAAGCCA 
GCTCATATCG CAAAGGTTAA GATGAAGTTT CGCTTCTTAG GAAAATTAAT GGCCAAGGCT 
ATCATGGATT TCAGATTGGT GGACCTTCCC CTTGGCTTAC CCTTTTATAA ATGGATGCTA 
CGGCAAGAAA CTTCACTGAC ATCACACGAT TTGTTTGACA TCGACCCAGT TGTAGCCAGA 
TCAGTTTATC ACCTAGAAGA CATTGTCAGA CAGAAGAAAA GACTTGAACA AGATAAATCC 
CAGACCAAAG AGAGTCTACA GTATGCATTA GAAACCTTGA CTATGAATGG CTGCTCAGTT 
GAAGATCTAG GACTGGATTT CACTCTGCCA GGGTTTCCCA ATATCGAACT GAAGAAAGGA 
GGGAAGGATA TACCAGTCAC TATCCACAAT TTAGAGGAGT ATCTAAGACT GGTTATATTC 
TGGGCACTAA ATGAAGGCGT TTCTAGGCAA TTTGATTCGT TCAGAGATGG ATTTGAATCA 
GTCTTCCCAC TCAGTCATCT TCAGTACTTC TACCCGGAGG AACTGGATCA GCTCCTTTGT 
GGCAGTAAAG CAGACACTTG GGATGCAAAG ACACTGATGG AATGCTGTAG GCCTGATCAT 
GGTTATACTC ATGACAGTCG GGCTGTGAAG TTTTTGTTTG AGATTCTCAG TAGTTTTGAT 
AATGAGCAGC AGAGGTTATT TCTCCAGTTT GTGACTGGTA GCCCAAGATT GCCTGTTGGA 
GGATTCCGGA GTTTGAATCC ACCTTTGACA ATTGTCCGAA AGACGTTTGA ATCAACAGAA 
AACCCAGATG ACTTCTTGCC CTCTGTAATG ACTTGTGTGA ACTATCTTAA GTTGCCGGAC 
TATTCAAGCA TTGAGATAAT GCGTGAAAAA CTGTTGATAG CAGCAAGAGA AGGGCAGCAG 
TCGTTCCATC TTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348330.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335259.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 20, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	NM_001348330.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_011512180.2. Transcript Variant: This variant (20), as well as variants 18 and 19, encodes the longest isoform (i). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.73539.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335259.1
CDS	168..6374
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348330.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348328.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335257.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 18, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	NM_001348328.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_005246954.4. Transcript Variant: This variant (18), as well as variants 19 and 20, encodes the longest isoform (i). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.185453.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335257.1
CDS	180..6386
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348328.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348328.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348329.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335258.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 19, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	NM_001348329.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005273.1. Transcript Variant: This variant (19), as well as variants 18 and 20, encodes the longest isoform (i). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.111587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335258.1
CDS	130..6336
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348329.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348329.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348330.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335259.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 20, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	NM_001348330.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348330.1. Transcript Variant: This variant (20), as well as variants 18 and 19, encodes the longest isoform (i). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.73539.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335259.1
CDS	569..6775
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348330.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348330.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348329.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335258.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 19, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	NM_001348329.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348329.1. Transcript Variant: This variant (19), as well as variants 18 and 20, encodes the longest isoform (i). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.111587.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335258.1
CDS	531..6737
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348329.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348329.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348322.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335251.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 12, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005277.1. Transcript Variant: This variant (12), as well as variants 13-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.111043.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335251.1
CDS	298..6501
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348322.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348322.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348327.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335256.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 17, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348327.1. Transcript Variant: This variant (17), as well as variants 12-16, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.39987.1, SRR1803616.136150.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335256.1
CDS	569..6772
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348327.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348327.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348324.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335253.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 14, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348324.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005275.1. Transcript Variant: This variant (14), as well as variants 12-13 and 15-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.157190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335253.1
CDS	166..6369
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348324.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348324.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348323.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335252.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 13, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348323.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_005246956.4. Transcript Variant: This variant (13), as well as variants 12 and 14-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.71354.1, SRR1803615.231040.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335252.1
CDS	180..6383
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348323.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348323.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348326.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335255.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 16, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348326.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005278.1. Transcript Variant: This variant (16), as well as variants 12-15 and 17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.150656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335255.1
CDS	165..6368
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348326.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348326.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348326.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335255.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 16, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348326.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348326.1. Transcript Variant: This variant (16), as well as variants 12-15 and 17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.150656.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335255.1
CDS	566..6769
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348326.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348326.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348324.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335253.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 14, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348324.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348324.1. Transcript Variant: This variant (14), as well as variants 12-13 and 15-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.157190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335253.1
CDS	276..6479
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348324.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348324.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348325.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335254.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 15, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348325.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. Transcript Variant: This variant (15), as well as variants 12-14 and 16-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.153181.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335254.1
CDS	290..6493
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348325.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348325.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348327.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335256.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 17, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348327.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_017005276.1. Transcript Variant: This variant (17), as well as variants 12-16, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.136150.1, SRR1803613.39987.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335256.1
CDS	168..6371
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348327.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348327.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348325.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335254.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 15, mRNA.

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CloneID	OHu36804
Clone ID Related Accession (Same CDS sequence)	NM_001348322.1 , NM_001348327.2 , NM_001348324.1 , NM_001348323.1 , NM_001348326.1 , NM_001348326.2 , NM_001348323.2 , NM_001348324.2 , NM_001348325.1 , NM_001348327.1 , NM_001348325.2
Accession Version	NM_001348325.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jun 2, 2019 this sequence version replaced NM_001348325.1. Transcript Variant: This variant (15), as well as variants 12-14 and 16-17, encodes isoform h. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.153181.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335254.1
CDS	691..6894
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348325.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348325.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGCAG CCCATCAGGT CGGTGAGGAT 
GAGATAAGCT TGTCCACTCT GGGACGAGTT TATACTATTG ATTTTAATTC TATGCAGCAA 
ATCAATGAGG ACACGGGAAC AGCACGTGCC ATTCAGAGAA AACCTAACCC GTTAGCCAAT 
AGTAACACTA GTGGATATTC AGAGTCAAAG AAGGATGATG CTCGAGCACA GCTTATGAAA 
GAGGATCCGG AACTGGCTAA GTCTTTTATT AAGACATTAT TTGGTGTTCT TTATGAAGTG 
TATAGTTCCT CAGCAGGACC TGCGGTCAGA CATAAGTGCC TTAGAGCAAT TCTTAGGATA 
ATTTATTTTG CGGATGCTGA ACTTCTGAAG GATGTTCTGA AAAATCATGC TGTTTCAAGT 
CACATTGCTT CCATGCTGTC AAGCCAAGAC CTGAAGATAG TAGTGGGAGC ACTTCAGATG 
GCAGAAATTT TAATGCAGAA GTTACCTGAT ATTTTTAGTG TTTACTTCAG AAGAGAAGGT 
GTAATGCATC AAGTAAAACA CTTAGCAGAA TCAGAGTCTT TGTTGACAAG TCCACCAAAG 
GCATGTACGA ATGGATCGGG ATCCATGGGA TCCACAACTT CAGTCAGCAG TGGGACAGCC 
ACAGCTGCCA CTCATGCTGC AGCTGACTTG GGATCACCCA GCTTGCAGCA CAGCAGGGAT 
GATTCTTTAG ATCTCAGCCC TCAAGGTCGA TTAAGTGATG TTCTAAAGAG AAAACGACTG 
CCAAAACGAG GGCCAAGAAG GCCAAAGTAC TCACCTCCAA GAGATGATGA CAAAGTAGAC 
AATCAAGCTA AAAGCCCCAC CACTACTCAG TCACCTAAAT CTTCTTTCCT GGCAAGCTTG 
AATCCAAAAA CATGGGGAAG GTTAAGTACA CAGTCCAACA GCAACAACAT TGAGCCAGCA 
CGGACTGCGG GAGGTAGTGG CCTTGCCAGG GCTGCCTCAA AGGATACCAT CTCCAATAAT 
AGAGAAAAAA TTAAAGGTTG GATTAAGGAG CAGGCACATA AATTTGTAGA ACGTTATTTC 
AGTTCTGAGA ATATGGATGG AAGCAACCCT GCATTGAATG TCCTTCAGAG ACTTTGTGCT 
GCAACCGAAC AACTCAACCT CCAGGTGGAT GGTGGAGCTG AGTGCCTTGT AGAAATCCGT 
AGCATAGTCT CAGAGTCAGA TGTTTCATCA TTTGAAATCC AACATAGTGG ATTTGTGAAG 
CAGCTGTTGC TTTATTTGAC ATCTAAAAGT GAAAAGGATG CTGTGAGCAG AGAGATCAGA 
TTAAAGCGAT TTCTTCATGT ATTTTTTTCT TCTCCACTTC CTGGAGAAGA GCCCATTGGA 
AGAGTGGAAC CAGTGGGTAA TGCACCTTTG TTGGCATTAG TTCACAAGAT GAACAACTGC 
CTCAGCCAGA TGGAACAATT TCCAGTCAAA GTACATGATT TCCCTAGTGG AAATGGGACA 
GGAGGCAGCT TTTCTCTCAA CAGAGGATCA CAGGCTTTAA AATTTTTCAA CACACATCAA 
TTAAAATGCC AGTTACAAAG GCATCCAGAC TGTGCAAATG TGAAGCAGTG GAAGGGTGGA 
CCTGTCAAGA TTGACCCTCT GGCTTTGGTA CAAGCCATCG AGAGATACCT TGTAGTTAGA 
GGGTATGGAA GAGTAAGAGA AGATGATGAA GACAGCGATG ACGATGGATC AGATGAGGAA 
ATAGATGAGT CTCTGGCTGC TCAGTTCCTA AATTCAGGAA ATGTAAGACA CAGGCTGCAG 
TTTTATATTG GAGAACATTT GCTGCCGTAT AACATGACTG TGTATCAGGC AGTACGGCAG 
TTTAGTATAC AGGCTGAAGA TGAAAGAGAA TCCACAGATG ATGAGAGCAA TCCTCTAGGC 
AGAGCTGGTA TTTGGACAAA GACTCATACA ATATGGTATA AACCTGTGAG AGAGGATGAA 
GAAAGTAATA AAGATTGTGT TGGTGGTAAA AGAGGAAGAG CCCAAACAGC TCCAACGAAA 
ACTTCCCCTA GAAATGCAAA AAAGCATGAT GAGTTATGGC ACGATGGAGT GTGCCCATCA 
GTATCAAATC CTTTAGAAGT TTACCTCATT CCCACACCAC CTGAAAATAT AACATTTGAA 
GACCCGTCAT TAGATGTGAT CCTTCTTTTA AGAGTTTTAC ATGCTATCAG TCGATACTGG 
TATTACTTGT ATGATAATGC AATGTGCAAG GAAATTATTC CAACTAGTGA ATTTATTAAC 
AGTAAGTTAA CAGCAAAAGC AAATAGGCAA CTTCAAGATC CTTTAGTAAT CATGACAGGA 
AACATCCCAA CATGGCTTAC TGAGCTAGGA AAAACCTGCC CATTTTTCTT TCCTTTTGAT 
ACCCGGCAAA TGCTTTTTTA TGTAACTGCA TTTGATCGGG ACCGAGCAAT GCAAAGATTA 
CTTGATACCA ACCCAGAAAT CAACCAGTCT GATTCTCAAG ATAGCAGAGT TGCACCTAGA 
TTGGATAGAA AAAAACGTAC TGTGAACCGA GAGGAGCTGC TGAAACAGGC GGAGTCTGTG 
ATGCAGGACC TCGGCAGCTC ACGGGCCATG TTAGAAATCC AGTATGAAAA TGAGGTTGGT 
ACAGGTCTTG GGCCTACACT GGAGTTTTAT GCGCTTGTAT CTCAGGAACT ACAGAGAGCT 
GACTTGGGTC TTTGGAGAGG TGAAGAAGTA ACTCTTAGCA ATCCAAAAGG GAGCCAAGAA 
GGGACCAAGT ATATTCAAAA CCTCCAGGGC CTGTTTGCGC TTCCCTTTGG TAGGACAGCA 
AAGCCAGCTC ATATCGCAAA GGTTAAGATG AAGTTTCGCT TCTTAGGAAA ATTAATGGCC 
AAGGCTATCA TGGATTTCAG ATTGGTGGAC CTTCCCCTTG GCTTACCCTT TTATAAATGG 
ATGCTACGGC AAGAAACTTC ACTGACATCA CACGATTTGT TTGACATCGA CCCAGTTGTA 
GCCAGATCAG TTTATCACCT AGAAGACATT GTCAGACAGA AGAAAAGACT TGAACAAGAT 
AAATCCCAGA CCAAAGAGAG TCTACAGTAT GCATTAGAAA CCTTGACTAT GAATGGCTGC 
TCAGTTGAAG ATCTAGGACT GGATTTCACT CTGCCAGGGT TTCCCAATAT CGAACTGAAG 
AAAGGAGGGA AGGATATACC AGTCACTATC CACAATTTAG AGGAGTATCT AAGACTGGTT 
ATATTCTGGG CACTAAATGA AGGCGTTTCT AGGCAATTTG ATTCGTTCAG AGATGGATTT 
GAATCAGTCT TCCCACTCAG TCATCTTCAG TACTTCTACC CGGAGGAACT GGATCAGCTC 
CTTTGTGGCA GTAAAGCAGA CACTTGGGAT GCAAAGACAC TGATGGAATG CTGTAGGCCT 
GATCATGGTT ATACTCATGA CAGTCGGGCT GTGAAGTTTT TGTTTGAGAT TCTCAGTAGT 
TTTGATAATG AGCAGCAGAG GTTATTTCTC CAGTTTGTGA CTGGTAGCCC AAGATTGCCT 
GTTGGAGGAT TCCGGAGTTT GAATCCACCT TTGACAATTG TCCGAAAGAC GTTTGAATCA 
ACAGAAAACC CAGATGACTT CTTGCCCTCT GTAATGACTT GTGTGAACTA TCTTAAGTTG 
CCGGACTATT CAAGCATTGA GATAATGCGT GAAAAACTGT TGATAGCAGC AAGAGAAGGG 
CAGCAGTCGT TCCATCTTTC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348321.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335250.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 11, mRNA.

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CloneID	OHu36805
Clone ID Related Accession (Same CDS sequence)	NM_001348321.1
Accession Version	NM_001348321.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_005246957.4. Transcript Variant: This variant (11) encodes isoform g. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.6139.1, SRR1803613.256234.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335250.1
CDS	180..6371
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348321.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348321.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GAGGATCACA GGCTTTAAAA TTTTTCAACA CACATCAATT AAAATGCCAG 
TTACAAAGGC ATCCAGACTG TGCAAATGTG AAGCAGTGGA AGGGTGGACC TGTCAAGATT 
GACCCTCTGG CTTTGGTACA AGCCATCGAG AGATACCTTG TAGTTAGAGG GTATGGAAGA 
GTAAGAGAAG ATGATGAAGA CAGCGATGAC GATGGATCAG ATGAGGAAAT AGATGAGTCT 
CTGGCTGCTC AGTTCCTAAA TTCAGGAAAT GTAAGACACA GGCTGCAGTT TTATATTGGA 
GAACATTTGC TGCCGTATAA CATGACTGTG TATCAGGCAG TACGGCAGTT TAGTATACAG 
GCTGAAGATG AAAGAGAATC CACAGATGAT GAGAGCAATC CTCTAGGCAG AGCTGGTATT 
TGGACAAAGA CTCATACAAT ATGGTATAAA CCTGTGAGAG AGGATGAAGA AAGTAATAAA 
GATTGTGTTG GTGGTAAAAG AGGAAGAGCC CAAACAGCTC CAACGAAAAC TTCCCCTAGA 
AATGCAAAAA AGCATGATGA GTTATGGCAC GATGGAGTGT GCCCATCAGT ATCAAATCCT 
TTAGAAGTTT ACCTCATTCC CACACCACCT GAAAATATAA CATTTGAAGA CCCGTCATTA 
GATGTGATCC TTCTTTTAAG AGTTTTACAT GCTATCAGTC GATACTGGTA TTACTTGTAT 
GATAATGCAA TGTGCAAGGA AATTATTCCA ACTAGTGAAT TTATTAACAG TAAGTTAACA 
GCAAAAGCAA ATAGGCAACT TCAAGATCCT TTAGTAATCA TGACAGGAAA CATCCCAACA 
TGGCTTACTG AGCTAGGAAA AACCTGCCCA TTTTTCTTTC CTTTTGATAC CCGGCAAATG 
CTTTTTTATG TAACTGCATT TGATCGGGAC CGAGCAATGC AAAGATTACT TGATACCAAC 
CCAGAAATCA ACCAGTCTGA TTCTCAAGAT AGCAGAGTTG CACCTAGATT GGATAGAAAA 
AAACGTACTG TGAACCGAGA GGAGCTGCTG AAACAGGCGG AGTCTGTGAT GCAGGACCTC 
GGCAGCTCAC GGGCCATGTT AGAAATCCAG TATGAAAATG AGGTTGGTAC AGGTCTTGGG 
CCTACACTGG AGTTTTATGC GCTTGTATCT CAGGAACTAC AGAGAGCTGA CTTGGGTCTT 
TGGAGAGGTG AAGAAGTAAC TCTTAGCAAT CCAAAAGGGA GCCAAGAAGG GACCAAGTAT 
ATTCAAAACC TCCAGGGCCT GTTTGCGCTT CCCTTTGGTA GGACAGCAAA GCCAGCTCAT 
ATCGCAAAGG TTAAGATGAA GTTTCGCTTC TTAGGAAAAT TAATGGCCAA GGCTATCATG 
GATTTCAGAT TGGTGGACCT TCCCCTTGGC TTACCCTTTT ATAAATGGAT GCTACGGCAA 
GAAACTTCAC TGACATCACA CGATTTGTTT GACATCGACC CAGTTGTAGC CAGATCAGTT 
TATCACCTAG AAGACATTGT CAGACAGAAG AAAAGACTTG AACAAGATAA ATCCCAGACC 
AAAGAGAGTC TACAGTATGC ATTAGAAACC TTGACTATGA ATGGCTGCTC AGTTGAAGAT 
CTAGGACTGG ATTTCACTCT GCCAGGGTTT CCCAATATCG AACTGAAGAA AGGAGGGAAG 
GATATACCAG TCACTATCCA CAATTTAGAG GAGTATCTAA GACTGGTTAT ATTCTGGGCA 
CTAAATGAAG GCGTTTCTAG GCAATTTGAT TCGTTCAGAG ATGGATTTGA ATCAGTCTTC 
CCACTCAGTC ATCTTCAGTA CTTCTACCCG GAGGAACTGG ATCAGCTCCT TTGTGGCAGT 
AAAGCAGACA CTTGGGATGC AAAGACACTG ATGGAATGCT GTAGGCCTGA TCATGGTTAT 
ACTCATGACA GTCGGGCTGT GAAGTTTTTG TTTGAGATTC TCAGTAGTTT TGATAATGAG 
CAGCAGAGGT TATTTCTCCA GTTTGTGACT GGTAGCCCAA GATTGCCTGT TGGAGGATTC 
CGGAGTTTGA ATCCACCTTT GACAATTGTC CGAAAGACGT TTGAATCAAC AGAAAACCCA 
GATGACTTCT TGCCCTCTGT AATGACTTGT GTGAACTATC TTAAGTTGCC GGACTATTCA 
AGCATTGAGA TAATGCGTGA AAAACTGTTG ATAGCAGCAA GAGAAGGGCA GCAGTCGTTC 
CATCTTTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( NM_001348333.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001335262.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 23, mRNA.

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CloneID	OHu36806
Clone ID Related Accession (Same CDS sequence)	NM_001348333.1
Accession Version	NM_001348333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009973.10, AC105380.5 and AC093384.4. On Jan 27, 2017 this sequence version replaced XM_005246958.4. Transcript Variant: This variant (23) encodes isoform l. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.108643.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCGTTAGCCA ATAGTAACAC TAGTGGATAT 
TCAGAGTCAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA GCACTTCAGA TGGCAGAAAT TTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGATCG 
GGATCCATGG GATCCACAAC TTCAGTCAGC AGTGGGACAG CCACAGCTGC CACTCATGCT 
GCAGCTGACT TGGGATCACC CAGCTTGCAG CACAGCAGGG ATGATTCTTT AGATCTCAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ACTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGTA CACAGTCCAA CAGCAACAAC ATTGAGCCAG CACGGACTGC GGGAGGTAGT 
GGCCTTGCCA GGGCTGCCTC AAAGGATACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAGG AGCAGGCACA TAAATTTGTA GAACGTTATT TCAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCAACCGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT GCTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC AGAGAGATCA GATTAAAGCG ATTTCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG GAAGAGTGGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGAAATGGGA CAGGAGGCAG CTTTTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGCT 
GCTCAGTTCC TAAATTCAGG AAATGTAAGA CACAGGCTGC AGTTTTATAT TGGAGAACAT 
TTGCTGCCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTTAGTAT ACAGGCTGAA 
GATGAAAGAG AATCCACAGA TGATGAGAGC AATCCTCTAG GCAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA TAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACA GCTCCAACGA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCACGATGGA GTGTGCCCAT CAGTATCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATAACATTTG AAGACCCGTC ATTAGATGTG 
ATCCTTCTTT TAAGAGTTTT ACATGCTATC AGTCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAAATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT AACAGCAAAA 
GCAAATAGGC AACTTCAAGA TCCTTTAGTA ATCATGACAG GAAACATCCC AACATGGCTT 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGGCA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACC GAGAGGAGCT GCTGAAACAG GCGGAGTCTG TGATGCAGGA CCTCGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA CTACAGAGAG CTGACTTGGG TCTTTGGAGA 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGGAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CAAAGCCAGC TCATATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGCTTACCC TTTTATAAAT GGATGCTACG GCAAGAAACT 
TCACTGACAT CACACGATTT GTTTGACATC GACCCAGTTG TAGCCAGATC AGTTTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAGT ATGCATTAGA AACCTTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
CTGGATTTCA CTCTGCCAGG GTTTCCCAAT ATCGAACTGA AGAAAGGAGG GAAGGATATA 
CCAGTCACTA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CTAGGCAATT TGATTCGTTC AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTTC AGTACTTCTA CCCGGAGGAA CTGGATCAGC TCCTTTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGTAGGC CTGATCATGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT TTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTATTTC TCCAGTTTGT GACTGGTAGC CCAAGATTGC CTGTTGGAGG ATTCCGGAGT 
TTGAATCCAC CTTTGACAAT TGTCCGAAAG ACGTTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCATT 
GAGATAATGC GTGAAAAACT GTTGATAGCA GCAAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001335262.1
CDS	180..6305
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	NM_001348333.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant 23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001348333.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAC AAATCAATGA GGACACGGGA 
ACAGCACGTG CCATTCAGAG AAAACCTAAC CCGTTAGCCA ATAGTAACAC TAGTGGATAT 
TCAGAGTCAA AGAAGGATGA TGCTCGAGCA CAGCTTATGA AAGAGGATCC GGAACTGGCT 
AAGTCTTTTA TTAAGACATT ATTTGGTGTT CTTTATGAAG TGTATAGTTC CTCAGCAGGA 
CCTGCGGTCA GACATAAGTG CCTTAGAGCA ATTCTTAGGA TAATTTATTT TGCGGATGCT 
GAACTTCTGA AGGATGTTCT GAAAAATCAT GCTGTTTCAA GTCACATTGC TTCCATGCTG 
TCAAGCCAAG ACCTGAAGAT AGTAGTGGGA GCACTTCAGA TGGCAGAAAT TTTAATGCAG 
AAGTTACCTG ATATTTTTAG TGTTTACTTC AGAAGAGAAG GTGTAATGCA TCAAGTAAAA 
CACTTAGCAG AATCAGAGTC TTTGTTGACA AGTCCACCAA AGGCATGTAC GAATGGATCG 
GGATCCATGG GATCCACAAC TTCAGTCAGC AGTGGGACAG CCACAGCTGC CACTCATGCT 
GCAGCTGACT TGGGATCACC CAGCTTGCAG CACAGCAGGG ATGATTCTTT AGATCTCAGC 
CCTCAAGGTC GATTAAGTGA TGTTCTAAAG AGAAAACGAC TGCCAAAACG AGGGCCAAGA 
AGGCCAAAGT ACTCACCTCC AAGAGATGAT GACAAAGTAG ACAATCAAGC TAAAAGCCCC 
ACCACTACTC AGTCACCTAA ATCTTCTTTC CTGGCAAGCT TGAATCCAAA AACATGGGGA 
AGGTTAAGTA CACAGTCCAA CAGCAACAAC ATTGAGCCAG CACGGACTGC GGGAGGTAGT 
GGCCTTGCCA GGGCTGCCTC AAAGGATACC ATCTCCAATA ATAGAGAAAA AATTAAAGGT 
TGGATTAAGG AGCAGGCACA TAAATTTGTA GAACGTTATT TCAGTTCTGA GAATATGGAT 
GGAAGCAACC CTGCATTGAA TGTCCTTCAG AGACTTTGTG CTGCAACCGA ACAACTCAAC 
CTCCAGGTGG ATGGTGGAGC TGAGTGCCTT GTAGAAATCC GTAGCATAGT CTCAGAGTCA 
GATGTTTCAT CATTTGAAAT CCAACATAGT GGATTTGTGA AGCAGCTGTT GCTTTATTTG 
ACATCTAAAA GTGAAAAGGA TGCTGTGAGC AGAGAGATCA GATTAAAGCG ATTTCTTCAT 
GTATTTTTTT CTTCTCCACT TCCTGGAGAA GAGCCCATTG GAAGAGTGGA ACCAGTGGGT 
AATGCACCTT TGTTGGCATT AGTTCACAAG ATGAACAACT GCCTCAGCCA GATGGAACAA 
TTTCCAGTCA AAGTACATGA TTTCCCTAGT GGAAATGGGA CAGGAGGCAG CTTTTCTCTC 
AACAGAGGAT CACAGGCTTT AAAATTTTTC AACACACATC AATTAAAATG CCAGTTACAA 
AGGCATCCAG ACTGTGCAAA TGTGAAGCAG TGGAAGGGTG GACCTGTCAA GATTGACCCT 
CTGGCTTTGG TACAAGCCAT CGAGAGATAC CTTGTAGTTA GAGGGTATGG AAGAGTAAGA 
GAAGATGATG AAGACAGCGA TGACGATGGA TCAGATGAGG AAATAGATGA GTCTCTGGCT 
GCTCAGTTCC TAAATTCAGG AAATGTAAGA CACAGGCTGC AGTTTTATAT TGGAGAACAT 
TTGCTGCCGT ATAACATGAC TGTGTATCAG GCAGTACGGC AGTTTAGTAT ACAGGCTGAA 
GATGAAAGAG AATCCACAGA TGATGAGAGC AATCCTCTAG GCAGAGCTGG TATTTGGACA 
AAGACTCATA CAATATGGTA TAAACCTGTG AGAGAGGATG AAGAAAGTAA TAAAGATTGT 
GTTGGTGGTA AAAGAGGAAG AGCCCAAACA GCTCCAACGA AAACTTCCCC TAGAAATGCA 
AAAAAGCATG ATGAGTTATG GCACGATGGA GTGTGCCCAT CAGTATCAAA TCCTTTAGAA 
GTTTACCTCA TTCCCACACC ACCTGAAAAT ATAACATTTG AAGACCCGTC ATTAGATGTG 
ATCCTTCTTT TAAGAGTTTT ACATGCTATC AGTCGATACT GGTATTACTT GTATGATAAT 
GCAATGTGCA AGGAAATTAT TCCAACTAGT GAATTTATTA ACAGTAAGTT AACAGCAAAA 
GCAAATAGGC AACTTCAAGA TCCTTTAGTA ATCATGACAG GAAACATCCC AACATGGCTT 
ACTGAGCTAG GAAAAACCTG CCCATTTTTC TTTCCTTTTG ATACCCGGCA AATGCTTTTT 
TATGTAACTG CATTTGATCG GGACCGAGCA ATGCAAAGAT TACTTGATAC CAACCCAGAA 
ATCAACCAGT CTGATTCTCA AGATAGCAGA GTTGCACCTA GATTGGATAG AAAAAAACGT 
ACTGTGAACC GAGAGGAGCT GCTGAAACAG GCGGAGTCTG TGATGCAGGA CCTCGGCAGC 
TCACGGGCCA TGTTAGAAAT CCAGTATGAA AATGAGGTTG GTACAGGTCT TGGGCCTACA 
CTGGAGTTTT ATGCGCTTGT ATCTCAGGAA CTACAGAGAG CTGACTTGGG TCTTTGGAGA 
GGTGAAGAAG TAACTCTTAG CAATCCAAAA GGGAGCCAAG AAGGGACCAA GTATATTCAA 
AACCTCCAGG GCCTGTTTGC GCTTCCCTTT GGTAGGACAG CAAAGCCAGC TCATATCGCA 
AAGGTTAAGA TGAAGTTTCG CTTCTTAGGA AAATTAATGG CCAAGGCTAT CATGGATTTC 
AGATTGGTGG ACCTTCCCCT TGGCTTACCC TTTTATAAAT GGATGCTACG GCAAGAAACT 
TCACTGACAT CACACGATTT GTTTGACATC GACCCAGTTG TAGCCAGATC AGTTTATCAC 
CTAGAAGACA TTGTCAGACA GAAGAAAAGA CTTGAACAAG ATAAATCCCA GACCAAAGAG 
AGTCTACAGT ATGCATTAGA AACCTTGACT ATGAATGGCT GCTCAGTTGA AGATCTAGGA 
CTGGATTTCA CTCTGCCAGG GTTTCCCAAT ATCGAACTGA AGAAAGGAGG GAAGGATATA 
CCAGTCACTA TCCACAATTT AGAGGAGTAT CTAAGACTGG TTATATTCTG GGCACTAAAT 
GAAGGCGTTT CTAGGCAATT TGATTCGTTC AGAGATGGAT TTGAATCAGT CTTCCCACTC 
AGTCATCTTC AGTACTTCTA CCCGGAGGAA CTGGATCAGC TCCTTTGTGG CAGTAAAGCA 
GACACTTGGG ATGCAAAGAC ACTGATGGAA TGCTGTAGGC CTGATCATGG TTATACTCAT 
GACAGTCGGG CTGTGAAGTT TTTGTTTGAG ATTCTCAGTA GTTTTGATAA TGAGCAGCAG 
AGGTTATTTC TCCAGTTTGT GACTGGTAGC CCAAGATTGC CTGTTGGAGG ATTCCGGAGT 
TTGAATCCAC CTTTGACAAT TGTCCGAAAG ACGTTTGAAT CAACAGAAAA CCCAGATGAC 
TTCTTGCCCT CTGTAATGAC TTGTGTGAAC TATCTTAAGT TGCCGGACTA TTCAAGCATT 
GAGATAATGC GTGAAAAACT GTTGATAGCA GCAAGAGAAG GGCAGCAGTC GTTCCATCTT 
TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_011512181.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510483.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	XM_011512181.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510483.1
CDS	155..6361
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011512181.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512181.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005272.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860761.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	XM_017005272.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860761.1
CDS	263..6469
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005272.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005272.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005246955.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247012.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	XM_005246955.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246955.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247012.1
CDS	203..6409
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005246955.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246955.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005274.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860763.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

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CloneID	OHu36803
Clone ID Related Accession (Same CDS sequence)	XM_011512181.2 , XM_017005272.1 , XM_005246955.4 , NM_001348330.1 , NM_001348328.1 , NM_001348329.1 , XM_017005274.1 , NM_001348330.2 , NM_001348329.2
Accession Version	XM_017005274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860763.1
CDS	78..6284
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005274.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005274.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GTAAAACTGG TGGCTCTTCA 
AAATTTGATT GGGCTGCTCG TTTCAGCCCT AAAGTTAGCC TTCCTAAAAC AAAACTGTCT 
CTTCCAGGGT CTTCTAAGTC AGAGACATCA AAACCTGGAC CTTCTGGATT ACAGGCCAAA 
TTAGCAAGTT TAAGAAAATC TACGAAGAAA CGCAGTGAGT CTCCACCTGC TGAGCTCCCC 
AGTTTGAGGC GGAGCACACG CCAAAAGACC ACGGGCTCCT GTGCTAGTAC CAGTCGGCGA 
GGCTCTGGCC TGGGCAAAAG AGGAGCAGCT GAAGCTCGTC GACAGGAGAA AATGGCAGAC 
CCTGAAAGCA ACCAGGAGGC AGTAAATTCT TCAGCTGCTC GGACAGATGA AGCTCCCCAA 
GGAGCTGCAG CCTCTAGTTC TGTTGCAGGG GCTGTTGGCA TGACCACCTC TGGGGAGAGT 
GAATCAGATG ATTCCGAGAT GGGACGTTTG CAAGCTTTGT TAGAGGCAAG GGGTCTTCCC 
CCTCACCTAT TTGGTCCTCT TGGTCCTCGG ATGTCACAGC TTTTCCATAG AACAATTGGA 
AGTGGAGCTA GTTCTAAGGC CCAGCAGCTA CTACAAGGAT TGCAAGCCAG TGATGAAAGT 
CAACAGCTTC AGGCAGTTAT TGAGATGTGT CAGTTACTGG TCATGGGAAA TGAGGAGACA 
CTGGGAGGGT TTCCTGTCAA GAGTGTTGTT CCAGCTTTGA TTACGTTACT TCAGATGGAG 
CACAATTTTG ATATTATGAA CCATGCTTGT CGAGCCTTAA CATACATGAT GGAAGCACTT 
CCTCGATCTT CTGCTGTTGT AGTAGATGCT ATTCCTGTCT TTTTAGAAAA GCTGCAAGTT 
ATTCAGTGTA TTGATGTGGC AGAGCAGGCC TTGACTGCCT TGGAGATGTT GTCACGGAGA 
CATAGTAAAG CCATTCTACA GGCGGGTGGT TTGGCAGACT GCTTGCTGTA CCTAGAATTC 
TTCAGCATAA ATGCCCAAAG AAATGCATTA GCAATTGCAG CTAATTGCTG CCAGAGTATC 
ACGCCAGATG AATTTCATTT TGTGGCAGAT TCACTCCCAT TGCTAACCCA AAGGCTAACA 
CATCAGGATA AAAAGTCAGT AGAAAGCACT TGCCTTTGTT TTGCACGCCT AGTGGACAAC 
TTCCAGCATG AGGAGAATTT ACTCCAGCAG GTTGCTTCCA AAGATCTGCT TACAAATGTT 
CAACAGCTGT TGGTAGTGAC TCCACCCATT TTAAGTTCTG GGATGTTTAT AATGGTGGTT 
CGCATGTTTT CTCTGATGTG TTCCAACTGT CCAACTTTAG CTGTTCAACT TATGAAACAA 
AACATTGCAG AAACGCTTCA CTTTCTCCTG TGTGGTGCCT CCAATGGAAG TTGTCAGGAA 
CAGATTGATC TTGTTCCACG AAGCCCTCAA GAGTTGTATG AACTGACATC TCTGATTTGT 
GAACTTATGC CATGTTTACC AAAAGAAGGC ATTTTTGCAG TTGATACCAT GTTGAAGAAG 
GGAAATGCAC AGAACACAGA TGGTGCGATA TGGCAGTGGC GTGATGATCG GGGCCTCTGG 
CATCCATATA ACAGGATTGA CAGCCGGATC ATTGAGGTAG CAGCCCATCA GGTCGGTGAG 
GATGAGATAA GCTTGTCCAC TCTGGGACGA GTTTATACTA TTGATTTTAA TTCTATGCAG 
CAAATCAATG AGGACACGGG AACAGCACGT GCCATTCAGA GAAAACCTAA CCCGTTAGCC 
AATAGTAACA CTAGTGGATA TTCAGAGTCA AAGAAGGATG ATGCTCGAGC ACAGCTTATG 
AAAGAGGATC CGGAACTGGC TAAGTCTTTT ATTAAGACAT TATTTGGTGT TCTTTATGAA 
GTGTATAGTT CCTCAGCAGG ACCTGCGGTC AGACATAAGT GCCTTAGAGC AATTCTTAGG 
ATAATTTATT TTGCGGATGC TGAACTTCTG AAGGATGTTC TGAAAAATCA TGCTGTTTCA 
AGTCACATTG CTTCCATGCT GTCAAGCCAA GACCTGAAGA TAGTAGTGGG AGCACTTCAG 
ATGGCAGAAA TTTTAATGCA GAAGTTACCT GATATTTTTA GTGTTTACTT CAGAAGAGAA 
GGTGTAATGC ATCAAGTAAA ACACTTAGCA GAATCAGAGT CTTTGTTGAC AAGTCCACCA 
AAGGCATGTA CGAATGGATC GGGATCCATG GGATCCACAA CTTCAGTCAG CAGTGGGACA 
GCCACAGCTG CCACTCATGC TGCAGCTGAC TTGGGATCAC CCAGCTTGCA GCACAGCAGG 
GATGATTCTT TAGATCTCAG CCCTCAAGGT CGATTAAGTG ATGTTCTAAA GAGAAAACGA 
CTGCCAAAAC GAGGGCCAAG AAGGCCAAAG TACTCACCTC CAAGAGATGA TGACAAAGTA 
GACAATCAAG CTAAAAGCCC CACCACTACT CAGTCACCTA AATCTTCTTT CCTGGCAAGC 
TTGAATCCAA AAACATGGGG AAGGTTAAGT ACACAGTCCA ACAGCAACAA CATTGAGCCA 
GCACGGACTG CGGGAGGTAG TGGCCTTGCC AGGGCTGCCT CAAAGGATAC CATCTCCAAT 
AATAGAGAAA AAATTAAAGG TTGGATTAAG GAGCAGGCAC ATAAATTTGT AGAACGTTAT 
TTCAGTTCTG AGAATATGGA TGGAAGCAAC CCTGCATTGA ATGTCCTTCA GAGACTTTGT 
GCTGCAACCG AACAACTCAA CCTCCAGGTG GATGGTGGAG CTGAGTGCCT TGTAGAAATC 
CGTAGCATAG TCTCAGAGTC AGATGTTTCA TCATTTGAAA TCCAACATAG TGGATTTGTG 
AAGCAGCTGT TGCTTTATTT GACATCTAAA AGTGAAAAGG ATGCTGTGAG CAGAGAGATC 
AGATTAAAGC GATTTCTTCA TGTATTTTTT TCTTCTCCAC TTCCTGGAGA AGAGCCCATT 
GGAAGAGTGG AACCAGTGGG TAATGCACCT TTGTTGGCAT TAGTTCACAA GATGAACAAC 
TGCCTCAGCC AGATGGAACA ATTTCCAGTC AAAGTACATG ATTTCCCTAG TGGAAATGGG 
ACAGGAGGCA GCTTTTCTCT CAACAGAGGA TCACAGGCTT TAAAATTTTT CAACACACAT 
CAATTAAAAT GCCAGTTACA AAGGCATCCA GACTGTGCAA ATGTGAAGCA GTGGAAGGGT 
GGACCTGTCA AGATTGACCC TCTGGCTTTG GTACAAGCCA TCGAGAGATA CCTTGTAGTT 
AGAGGGTATG GAAGAGTAAG AGAAGATGAT GAAGACAGCG ATGACGATGG ATCAGATGAG 
GAAATAGATG AGTCTCTGGC TGCTCAGTTC CTAAATTCAG GAAATGTAAG ACACAGGCTG 
CAGTTTTATA TTGGAGAACA TTTGCTGCCG TATAACATGA CTGTGTATCA GGCAGTACGG 
CAGTTTAGTA TACAGGCTGA AGATGAAAGA GAATCCACAG ATGATGAGAG CAATCCTCTA 
GGCAGAGCTG GTATTTGGAC AAAGACTCAT ACAATATGGT ATAAACCTGT GAGAGAGGAT 
GAAGAAAGTA ATAAAGATTG TGTTGGTGGT AAAAGAGGAA GAGCCCAAAC AGCTCCAACG 
AAAACTTCCC CTAGAAATGC AAAAAAGCAT GATGAGTTAT GGCACGATGG AGTGTGCCCA 
TCAGTATCAA ATCCTTTAGA AGTTTACCTC ATTCCCACAC CACCTGAAAA TATAACATTT 
GAAGACCCGT CATTAGATGT GATCCTTCTT TTAAGAGTTT TACATGCTAT CAGTCGATAC 
TGGTATTACT TGTATGATAA TGCAATGTGC AAGGAAATTA TTCCAACTAG TGAATTTATT 
AACAGTAAGT TAACAGCAAA AGCAAATAGG CAACTTCAAG ATCCTTTAGT AATCATGACA 
GGAAACATCC CAACATGGCT TACTGAGCTA GGAAAAACCT GCCCATTTTT CTTTCCTTTT 
GATACCCGGC AAATGCTTTT TTATGTAACT GCATTTGATC GGGACCGAGC AATGCAAAGA 
TTACTTGATA CCAACCCAGA AATCAACCAG TCTGATTCTC AAGATAGCAG AGTTGCACCT 
AGATTGGATA GAAAAAAACG TACTGTGAAC CGAGAGGAGC TGCTGAAACA GGCGGAGTCT 
GTGATGCAGG ACCTCGGCAG CTCACGGGCC ATGTTAGAAA TCCAGTATGA AAATGAGGTT 
GGTACAGGTC TTGGGCCTAC ACTGGAGTTT TATGCGCTTG TATCTCAGGA ACTACAGAGA 
GCTGACTTGG GTCTTTGGAG AGGTGAAGAA GTAACTCTTA GCAATCCAAA AGGGAGCCAA 
GAAGGGACCA AGTATATTCA AAACCTCCAG GGCCTGTTTG CGCTTCCCTT TGGTAGGACA 
GCAAAGCCAG CTCATATCGC AAAGGTTAAG ATGAAGTTTC GCTTCTTAGG AAAATTAATG 
GCCAAGGCTA TCATGGATTT CAGATTGGTG GACCTTCCCC TTGGCTTACC CTTTTATAAA 
TGGATGCTAC GGCAAGAAAC TTCACTGACA TCACACGATT TGTTTGACAT CGACCCAGTT 
GTAGCCAGAT CAGTTTATCA CCTAGAAGAC ATTGTCAGAC AGAAGAAAAG ACTTGAACAA 
GATAAATCCC AGACCAAAGA GAGTCTACAG TATGCATTAG AAACCTTGAC TATGAATGGC 
TGCTCAGTTG AAGATCTAGG ACTGGATTTC ACTCTGCCAG GGTTTCCCAA TATCGAACTG 
AAGAAAGGAG GGAAGGATAT ACCAGTCACT ATCCACAATT TAGAGGAGTA TCTAAGACTG 
GTTATATTCT GGGCACTAAA TGAAGGCGTT TCTAGGCAAT TTGATTCGTT CAGAGATGGA 
TTTGAATCAG TCTTCCCACT CAGTCATCTT CAGTACTTCT ACCCGGAGGA ACTGGATCAG 
CTCCTTTGTG GCAGTAAAGC AGACACTTGG GATGCAAAGA CACTGATGGA ATGCTGTAGG 
CCTGATCATG GTTATACTCA TGACAGTCGG GCTGTGAAGT TTTTGTTTGA GATTCTCAGT 
AGTTTTGATA ATGAGCAGCA GAGGTTATTT CTCCAGTTTG TGACTGGTAG CCCAAGATTG 
CCTGTTGGAG GATTCCGGAG TTTGAATCCA CCTTTGACAA TTGTCCGAAA GACGTTTGAA 
TCAACAGAAA ACCCAGATGA CTTCTTGCCC TCTGTAATGA CTTGTGTGAA CTATCTTAAG 
TTGCCGGACT ATTCAAGCAT TGAGATAATG CGTGAAAAAC TGTTGATAGC AGCAAGAGAA 
GGGCAGCAGT CGTTCCATCT TTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005246960.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247017.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

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CloneID	OHu36807
Clone ID Related Accession (Same CDS sequence)	XM_005246960.4 , XM_017005282.1
Accession Version	XM_005246960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246960.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGCTTTTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CATCAATTAA AATGCCAGTT ACAAAGGCAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGTGGACCTG TCAAGATTGA CCCTCTGGCT 
TTGGTACAAG CCATCGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GATGAAGACA GCGATGACGA TGGATCAGAT GAGGAAATAG ATGAGTCTCT GGCTGCTCAG 
TTCCTAAATT CAGGAAATGT AAGACACAGG CTGCAGTTTT ATATTGGAGA ACATTTGCTG 
CCGTATAACA TGACTGTGTA TCAGGCAGTA CGGCAGTTTA GTATACAGGC TGAAGATGAA 
AGAGAATCCA CAGATGATGA GAGCAATCCT CTAGGCAGAG CTGGTATTTG GACAAAGACT 
CATACAATAT GGTATAAACC TGTGAGAGAG GATGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA AACAGCTCCA ACGAAAACTT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGT TATGGCACGA TGGAGTGTGC CCATCAGTAT CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATAACA TTTGAAGACC CGTCATTAGA TGTGATCCTT 
CTTTTAAGAG TTTTACATGC TATCAGTCGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAAA TTATTCCAAC TAGTGAATTT ATTAACAGTA AGTTAACAGC AAAAGCAAAT 
AGGCAACTTC AAGATCCTTT AGTAATCATG ACAGGAAACA TCCCAACATG GCTTACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GGCAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACCGAGAGG AGCTGCTGAA ACAGGCGGAG TCTGTGATGC AGGACCTCGG CAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGTACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCGC TTGTATCTCA GGAACTACAG AGAGCTGACT TGGGTCTTTG GAGAGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGGAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCAAAGC CAGCTCATAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTTGGCTT ACCCTTTTAT AAATGGATGC TACGGCAAGA AACTTCACTG 
ACATCACACG ATTTGTTTGA CATCGACCCA GTTGTAGCCA GATCAGTTTA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTTGAA CAAGATAAAT CCCAGACCAA AGAGAGTCTA 
CAGTATGCAT TAGAAACCTT GACTATGAAT GGCTGCTCAG TTGAAGATCT AGGACTGGAT 
TTCACTCTGC CAGGGTTTCC CAATATCGAA CTGAAGAAAG GAGGGAAGGA TATACCAGTC 
ACTATCCACA ATTTAGAGGA GTATCTAAGA CTGGTTATAT TCTGGGCACT AAATGAAGGC 
GTTTCTAGGC AATTTGATTC GTTCAGAGAT GGATTTGAAT CAGTCTTCCC ACTCAGTCAT 
CTTCAGTACT TCTACCCGGA GGAACTGGAT CAGCTCCTTT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACACTGAT GGAATGCTGT AGGCCTGATC ATGGTTATAC TCATGACAGT 
CGGGCTGTGA AGTTTTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTA 
TTTCTCCAGT TTGTGACTGG TAGCCCAAGA TTGCCTGTTG GAGGATTCCG GAGTTTGAAT 
CCACCTTTGA CAATTGTCCG AAAGACGTTT GAATCAACAG AAAACCCAGA TGACTTCTTG 
CCCTCTGTAA TGACTTGTGT GAACTATCTT AAGTTGCCGG ACTATTCAAG CATTGAGATA 
ATGCGTGAAA AACTGTTGAT AGCAGCAAGA GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247017.1
CDS	204..6323
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005246960.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246960.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGCTTTTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CATCAATTAA AATGCCAGTT ACAAAGGCAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGTGGACCTG TCAAGATTGA CCCTCTGGCT 
TTGGTACAAG CCATCGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GATGAAGACA GCGATGACGA TGGATCAGAT GAGGAAATAG ATGAGTCTCT GGCTGCTCAG 
TTCCTAAATT CAGGAAATGT AAGACACAGG CTGCAGTTTT ATATTGGAGA ACATTTGCTG 
CCGTATAACA TGACTGTGTA TCAGGCAGTA CGGCAGTTTA GTATACAGGC TGAAGATGAA 
AGAGAATCCA CAGATGATGA GAGCAATCCT CTAGGCAGAG CTGGTATTTG GACAAAGACT 
CATACAATAT GGTATAAACC TGTGAGAGAG GATGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA AACAGCTCCA ACGAAAACTT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGT TATGGCACGA TGGAGTGTGC CCATCAGTAT CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATAACA TTTGAAGACC CGTCATTAGA TGTGATCCTT 
CTTTTAAGAG TTTTACATGC TATCAGTCGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAAA TTATTCCAAC TAGTGAATTT ATTAACAGTA AGTTAACAGC AAAAGCAAAT 
AGGCAACTTC AAGATCCTTT AGTAATCATG ACAGGAAACA TCCCAACATG GCTTACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GGCAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACCGAGAGG AGCTGCTGAA ACAGGCGGAG TCTGTGATGC AGGACCTCGG CAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGTACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCGC TTGTATCTCA GGAACTACAG AGAGCTGACT TGGGTCTTTG GAGAGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGGAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCAAAGC CAGCTCATAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTTGGCTT ACCCTTTTAT AAATGGATGC TACGGCAAGA AACTTCACTG 
ACATCACACG ATTTGTTTGA CATCGACCCA GTTGTAGCCA GATCAGTTTA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTTGAA CAAGATAAAT CCCAGACCAA AGAGAGTCTA 
CAGTATGCAT TAGAAACCTT GACTATGAAT GGCTGCTCAG TTGAAGATCT AGGACTGGAT 
TTCACTCTGC CAGGGTTTCC CAATATCGAA CTGAAGAAAG GAGGGAAGGA TATACCAGTC 
ACTATCCACA ATTTAGAGGA GTATCTAAGA CTGGTTATAT TCTGGGCACT AAATGAAGGC 
GTTTCTAGGC AATTTGATTC GTTCAGAGAT GGATTTGAAT CAGTCTTCCC ACTCAGTCAT 
CTTCAGTACT TCTACCCGGA GGAACTGGAT CAGCTCCTTT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACACTGAT GGAATGCTGT AGGCCTGATC ATGGTTATAC TCATGACAGT 
CGGGCTGTGA AGTTTTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTA 
TTTCTCCAGT TTGTGACTGG TAGCCCAAGA TTGCCTGTTG GAGGATTCCG GAGTTTGAAT 
CCACCTTTGA CAATTGTCCG AAAGACGTTT GAATCAACAG AAAACCCAGA TGACTTCTTG 
CCCTCTGTAA TGACTTGTGT GAACTATCTT AAGTTGCCGG ACTATTCAAG CATTGAGATA 
ATGCGTGAAA AACTGTTGAT AGCAGCAAGA GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005282.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860771.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

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CloneID	OHu36807
Clone ID Related Accession (Same CDS sequence)	XM_005246960.4 , XM_017005282.1
Accession Version	XM_017005282.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGCTTTTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CATCAATTAA AATGCCAGTT ACAAAGGCAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGTGGACCTG TCAAGATTGA CCCTCTGGCT 
TTGGTACAAG CCATCGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GATGAAGACA GCGATGACGA TGGATCAGAT GAGGAAATAG ATGAGTCTCT GGCTGCTCAG 
TTCCTAAATT CAGGAAATGT AAGACACAGG CTGCAGTTTT ATATTGGAGA ACATTTGCTG 
CCGTATAACA TGACTGTGTA TCAGGCAGTA CGGCAGTTTA GTATACAGGC TGAAGATGAA 
AGAGAATCCA CAGATGATGA GAGCAATCCT CTAGGCAGAG CTGGTATTTG GACAAAGACT 
CATACAATAT GGTATAAACC TGTGAGAGAG GATGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA AACAGCTCCA ACGAAAACTT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGT TATGGCACGA TGGAGTGTGC CCATCAGTAT CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATAACA TTTGAAGACC CGTCATTAGA TGTGATCCTT 
CTTTTAAGAG TTTTACATGC TATCAGTCGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAAA TTATTCCAAC TAGTGAATTT ATTAACAGTA AGTTAACAGC AAAAGCAAAT 
AGGCAACTTC AAGATCCTTT AGTAATCATG ACAGGAAACA TCCCAACATG GCTTACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GGCAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACCGAGAGG AGCTGCTGAA ACAGGCGGAG TCTGTGATGC AGGACCTCGG CAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGTACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCGC TTGTATCTCA GGAACTACAG AGAGCTGACT TGGGTCTTTG GAGAGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGGAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCAAAGC CAGCTCATAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTTGGCTT ACCCTTTTAT AAATGGATGC TACGGCAAGA AACTTCACTG 
ACATCACACG ATTTGTTTGA CATCGACCCA GTTGTAGCCA GATCAGTTTA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTTGAA CAAGATAAAT CCCAGACCAA AGAGAGTCTA 
CAGTATGCAT TAGAAACCTT GACTATGAAT GGCTGCTCAG TTGAAGATCT AGGACTGGAT 
TTCACTCTGC CAGGGTTTCC CAATATCGAA CTGAAGAAAG GAGGGAAGGA TATACCAGTC 
ACTATCCACA ATTTAGAGGA GTATCTAAGA CTGGTTATAT TCTGGGCACT AAATGAAGGC 
GTTTCTAGGC AATTTGATTC GTTCAGAGAT GGATTTGAAT CAGTCTTCCC ACTCAGTCAT 
CTTCAGTACT TCTACCCGGA GGAACTGGAT CAGCTCCTTT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACACTGAT GGAATGCTGT AGGCCTGATC ATGGTTATAC TCATGACAGT 
CGGGCTGTGA AGTTTTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTA 
TTTCTCCAGT TTGTGACTGG TAGCCCAAGA TTGCCTGTTG GAGGATTCCG GAGTTTGAAT 
CCACCTTTGA CAATTGTCCG AAAGACGTTT GAATCAACAG AAAACCCAGA TGACTTCTTG 
CCCTCTGTAA TGACTTGTGT GAACTATCTT AAGTTGCCGG ACTATTCAAG CATTGAGATA 
ATGCGTGAAA AACTGTTGAT AGCAGCAAGA GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860771.1
CDS	203..6322
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSHLGQAKHKGYSPPESRKSNSKAPKVQSNTTSELSRGHLSKRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQRSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005282.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005282.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGGT CACATTTAGG GCAGGCAAAA 
CATAAGGGAT ATAGCCCTCC TGAGAGTAGA AAATCTAATT CTAAGGCACC CAAAGTGCAG 
TCTAATACTA CTTCTGAACT GTCAAGGGGA CACCTTTCTA AAAGAAGCTG CAGTTCATCA 
TCTGCTGTGA TAGTTCCACA ACCAGAGGAT CCAGACAGAG CCAATACTTC AGAAAGACAA 
AAAACGGGGC AGGTGCCTAA GAAAGACAAT TCTCGAGGAG TGAAGCGCAG TGCTAGTCCA 
GACTACAACA GGACCAATTC TCCTAGCTCT GCAAAAAAAC CAAAAGCACT TCAGCATACT 
GAATCTCCCT CAGAAACAAA TAAGCCACAT AGTAAGTCAA AGAAGAGACA TTTAGACCAG 
GAGCAACAAC TGAAATCTGC ACAATCACCA TCAACAAGCA AGGCTCATAC CAGGAAGAGT 
GGGGCCACTG GCGGTTCACG GAGTCAGAAA AGAAAAAGGA CAGAGAGTTC TTGTGTAAAG 
AGTGGCTCCG GGTCTGAATC AACTGGTGCA GAAGAGAGAT CTGCGAAACC TACCAAGCTG 
GCTTCAAAAT CAGCCACCTC AGCCAAAGCT GGGTGTAGCA CCATCACTGA TTCTTCTTCT 
GCTGCCTCTA CTTCCTCCTC GTCTTCTGCT GTAGCCTCGG CCTCCTCCAC TGTACCACCA 
GGTGCCAGAG TGAAACAAGG AAAAGATCAG AACAAGGCCA GGCGTTCCCG TTCAGCGTCC 
AGTCCCAGCC CCAGAAGAAG TAGCAGGGAA AAGGAACAGA GGTCTTCTAA GTCAGAGACA 
TCAAAACCTG GACCTTCTGG ATTACAGGCC AAATTAGCAA GTTTAAGAAA ATCTACGAAG 
AAACGCAGTG AGTCTCCACC TGCTGAGCTC CCCAGTTTGA GGCGGAGCAC ACGCCAAAAG 
ACCACGGGCT CCTGTGCTAG TACCAGTCGG CGAGGCTCTG GCCTGGGCAA AAGAGGAGCA 
GCTGAAGCTC GTCGACAGGA GAAAATGGCA GACCCTGAAA GCAACCAGGA GGCAGTAAAT 
TCTTCAGCTG CTCGGACAGA TGAAGCTCCC CAAGGAGCTG CAGCCTCTAG TTCTGTTGCA 
GGGGCTGTTG GCATGACCAC CTCTGGGGAG AGTGAATCAG ATGATTCCGA GATGGGACGT 
TTGCAAGCTT TGTTAGAGGC AAGGGGTCTT CCCCCTCACC TATTTGGTCC TCTTGGTCCT 
CGGATGTCAC AGCTTTTCCA TAGAACAATT GGAAGTGGAG CTAGTTCTAA GGCCCAGCAG 
CTACTACAAG GATTGCAAGC CAGTGATGAA AGTCAACAGC TTCAGGCAGT TATTGAGATG 
TGTCAGTTAC TGGTCATGGG AAATGAGGAG ACACTGGGAG GGTTTCCTGT CAAGAGTGTT 
GTTCCAGCTT TGATTACGTT ACTTCAGATG GAGCACAATT TTGATATTAT GAACCATGCT 
TGTCGAGCCT TAACATACAT GATGGAAGCA CTTCCTCGAT CTTCTGCTGT TGTAGTAGAT 
GCTATTCCTG TCTTTTTAGA AAAGCTGCAA GTTATTCAGT GTATTGATGT GGCAGAGCAG 
GCCTTGACTG CCTTGGAGAT GTTGTCACGG AGACATAGTA AAGCCATTCT ACAGGCGGGT 
GGTTTGGCAG ACTGCTTGCT GTACCTAGAA TTCTTCAGCA TAAATGCCCA AAGAAATGCA 
TTAGCAATTG CAGCTAATTG CTGCCAGAGT ATCACGCCAG ATGAATTTCA TTTTGTGGCA 
GATTCACTCC CATTGCTAAC CCAAAGGCTA ACACATCAGG ATAAAAAGTC AGTAGAAAGC 
ACTTGCCTTT GTTTTGCACG CCTAGTGGAC AACTTCCAGC ATGAGGAGAA TTTACTCCAG 
CAGGTTGCTT CCAAAGATCT GCTTACAAAT GTTCAACAGC TGTTGGTAGT GACTCCACCC 
ATTTTAAGTT CTGGGATGTT TATAATGGTG GTTCGCATGT TTTCTCTGAT GTGTTCCAAC 
TGTCCAACTT TAGCTGTTCA ACTTATGAAA CAAAACATTG CAGAAACGCT TCACTTTCTC 
CTGTGTGGTG CCTCCAATGG AAGTTGTCAG GAACAGATTG ATCTTGTTCC ACGAAGCCCT 
CAAGAGTTGT ATGAACTGAC ATCTCTGATT TGTGAACTTA TGCCATGTTT ACCAAAAGAA 
GGCATTTTTG CAGTTGATAC CATGTTGAAG AAGGGAAATG CACAGAACAC AGATGGTGCG 
ATATGGCAGT GGCGTGATGA TCGGGGCCTC TGGCATCCAT ATAACAGGAT TGACAGCCGG 
ATCATTGAGG TAGCAGCCCA TCAGGTCGGT GAGGATGAGA TAAGCTTGTC CACTCTGGGA 
CGAGTTTATA CTATTGATTT TAATTCTATG CAGCAAATCA ATGAGGACAC GGGAACAGCA 
CGTGCCATTC AGAGAAAACC TAACCCGTTA GCCAATAGTA ACACTAGTGG ATATTCAGAG 
TCAAAGAAGG ATGATGCTCG AGCACAGCTT ATGAAAGAGG ATCCGGAACT GGCTAAGTCT 
TTTATTAAGA CATTATTTGG TGTTCTTTAT GAAGTGTATA GTTCCTCAGC AGGACCTGCG 
GTCAGACATA AGTGCCTTAG AGCAATTCTT AGGATAATTT ATTTTGCGGA TGCTGAACTT 
CTGAAGGATG TTCTGAAAAA TCATGCTGTT TCAAGTCACA TTGCTTCCAT GCTGTCAAGC 
CAAGACCTGA AGATAGTAGT GGGAGCACTT CAGATGGCAG AAATTTTAAT GCAGAAGTTA 
CCTGATATTT TTAGTGTTTA CTTCAGAAGA GAAGGTGTAA TGCATCAAGT AAAACACTTA 
GCAGAATCAG AGTCTTTGTT GACAAGTCCA CCAAAGGCAT GTACGAATGG ATCGGGATCC 
ATGGGATCCA CAACTTCAGT CAGCAGTGGG ACAGCCACAG CTGCCACTCA TGCTGCAGCT 
GACTTGGGAT CACCCAGCTT GCAGCACAGC AGGGATGATT CTTTAGATCT CAGCCCTCAA 
GGTCGATTAA GTGATGTTCT AAAGAGAAAA CGACTGCCAA AACGAGGGCC AAGAAGGCCA 
AAGTACTCAC CTCCAAGAGA TGATGACAAA GTAGACAATC AAGCTAAAAG CCCCACCACT 
ACTCAGTCAC CTAAATCTTC TTTCCTGGCA AGCTTGAATC CAAAAACATG GGGAAGGTTA 
AGTACACAGT CCAACAGCAA CAACATTGAG CCAGCACGGA CTGCGGGAGG TAGTGGCCTT 
GCCAGGGCTG CCTCAAAGGA TACCATCTCC AATAATAGAG AAAAAATTAA AGGTTGGATT 
AAGGAGCAGG CACATAAATT TGTAGAACGT TATTTCAGTT CTGAGAATAT GGATGGAAGC 
AACCCTGCAT TGAATGTCCT TCAGAGACTT TGTGCTGCAA CCGAACAACT CAACCTCCAG 
GTGGATGGTG GAGCTGAGTG CCTTGTAGAA ATCCGTAGCA TAGTCTCAGA GTCAGATGTT 
TCATCATTTG AAATCCAACA TAGTGGATTT GTGAAGCAGC TGTTGCTTTA TTTGACATCT 
AAAAGTGAAA AGGATGCTGT GAGCAGAGAG ATCAGATTAA AGCGATTTCT TCATGTATTT 
TTTTCTTCTC CACTTCCTGG AGAAGAGCCC ATTGGAAGAG TGGAACCAGT GGGTAATGCA 
CCTTTGTTGG CATTAGTTCA CAAGATGAAC AACTGCCTCA GCCAGATGGA ACAATTTCCA 
GTCAAAGTAC ATGATTTCCC TAGTGGAAAT GGGACAGGAG GCAGCTTTTC TCTCAACAGA 
GGATCACAGG CTTTAAAATT TTTCAACACA CATCAATTAA AATGCCAGTT ACAAAGGCAT 
CCAGACTGTG CAAATGTGAA GCAGTGGAAG GGTGGACCTG TCAAGATTGA CCCTCTGGCT 
TTGGTACAAG CCATCGAGAG ATACCTTGTA GTTAGAGGGT ATGGAAGAGT AAGAGAAGAT 
GATGAAGACA GCGATGACGA TGGATCAGAT GAGGAAATAG ATGAGTCTCT GGCTGCTCAG 
TTCCTAAATT CAGGAAATGT AAGACACAGG CTGCAGTTTT ATATTGGAGA ACATTTGCTG 
CCGTATAACA TGACTGTGTA TCAGGCAGTA CGGCAGTTTA GTATACAGGC TGAAGATGAA 
AGAGAATCCA CAGATGATGA GAGCAATCCT CTAGGCAGAG CTGGTATTTG GACAAAGACT 
CATACAATAT GGTATAAACC TGTGAGAGAG GATGAAGAAA GTAATAAAGA TTGTGTTGGT 
GGTAAAAGAG GAAGAGCCCA AACAGCTCCA ACGAAAACTT CCCCTAGAAA TGCAAAAAAG 
CATGATGAGT TATGGCACGA TGGAGTGTGC CCATCAGTAT CAAATCCTTT AGAAGTTTAC 
CTCATTCCCA CACCACCTGA AAATATAACA TTTGAAGACC CGTCATTAGA TGTGATCCTT 
CTTTTAAGAG TTTTACATGC TATCAGTCGA TACTGGTATT ACTTGTATGA TAATGCAATG 
TGCAAGGAAA TTATTCCAAC TAGTGAATTT ATTAACAGTA AGTTAACAGC AAAAGCAAAT 
AGGCAACTTC AAGATCCTTT AGTAATCATG ACAGGAAACA TCCCAACATG GCTTACTGAG 
CTAGGAAAAA CCTGCCCATT TTTCTTTCCT TTTGATACCC GGCAAATGCT TTTTTATGTA 
ACTGCATTTG ATCGGGACCG AGCAATGCAA AGATTACTTG ATACCAACCC AGAAATCAAC 
CAGTCTGATT CTCAAGATAG CAGAGTTGCA CCTAGATTGG ATAGAAAAAA ACGTACTGTG 
AACCGAGAGG AGCTGCTGAA ACAGGCGGAG TCTGTGATGC AGGACCTCGG CAGCTCACGG 
GCCATGTTAG AAATCCAGTA TGAAAATGAG GTTGGTACAG GTCTTGGGCC TACACTGGAG 
TTTTATGCGC TTGTATCTCA GGAACTACAG AGAGCTGACT TGGGTCTTTG GAGAGGTGAA 
GAAGTAACTC TTAGCAATCC AAAAGGGAGC CAAGAAGGGA CCAAGTATAT TCAAAACCTC 
CAGGGCCTGT TTGCGCTTCC CTTTGGTAGG ACAGCAAAGC CAGCTCATAT CGCAAAGGTT 
AAGATGAAGT TTCGCTTCTT AGGAAAATTA ATGGCCAAGG CTATCATGGA TTTCAGATTG 
GTGGACCTTC CCCTTGGCTT ACCCTTTTAT AAATGGATGC TACGGCAAGA AACTTCACTG 
ACATCACACG ATTTGTTTGA CATCGACCCA GTTGTAGCCA GATCAGTTTA TCACCTAGAA 
GACATTGTCA GACAGAAGAA AAGACTTGAA CAAGATAAAT CCCAGACCAA AGAGAGTCTA 
CAGTATGCAT TAGAAACCTT GACTATGAAT GGCTGCTCAG TTGAAGATCT AGGACTGGAT 
TTCACTCTGC CAGGGTTTCC CAATATCGAA CTGAAGAAAG GAGGGAAGGA TATACCAGTC 
ACTATCCACA ATTTAGAGGA GTATCTAAGA CTGGTTATAT TCTGGGCACT AAATGAAGGC 
GTTTCTAGGC AATTTGATTC GTTCAGAGAT GGATTTGAAT CAGTCTTCCC ACTCAGTCAT 
CTTCAGTACT TCTACCCGGA GGAACTGGAT CAGCTCCTTT GTGGCAGTAA AGCAGACACT 
TGGGATGCAA AGACACTGAT GGAATGCTGT AGGCCTGATC ATGGTTATAC TCATGACAGT 
CGGGCTGTGA AGTTTTTGTT TGAGATTCTC AGTAGTTTTG ATAATGAGCA GCAGAGGTTA 
TTTCTCCAGT TTGTGACTGG TAGCCCAAGA TTGCCTGTTG GAGGATTCCG GAGTTTGAAT 
CCACCTTTGA CAATTGTCCG AAAGACGTTT GAATCAACAG AAAACCCAGA TGACTTCTTG 
CCCTCTGTAA TGACTTGTGT GAACTATCTT AAGTTGCCGG ACTATTCAAG CATTGAGATA 
ATGCGTGAAA AACTGTTGAT AGCAGCAAGA GAAGGGCAGC AGTCGTTCCA TCTTTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005246961.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247018.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.

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CloneID	OHu36808
Clone ID Related Accession (Same CDS sequence)	XM_005246961.3 , XM_011512183.2 , XM_006712852.3 , XM_017005285.1 , XM_005246961.4
Accession Version	XM_005246961.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005246961.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247018.1
CDS	180..6260
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005246961.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246961.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_011512183.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011510485.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.

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CloneID	OHu36808
Clone ID Related Accession (Same CDS sequence)	XM_005246961.3 , XM_011512183.2 , XM_006712852.3 , XM_017005285.1 , XM_005246961.4
Accession Version	XM_011512183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011510485.1
CDS	137..6217
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_011512183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512183.2

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_006712852.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006712915.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.

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CloneID	OHu36808
Clone ID Related Accession (Same CDS sequence)	XM_005246961.3 , XM_011512183.2 , XM_006712852.3 , XM_017005285.1 , XM_005246961.4
Accession Version	XM_006712852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006712915.1
CDS	133..6213
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_006712852.3
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006712852.3

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_017005285.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860774.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.

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CloneID	OHu36808
Clone ID Related Accession (Same CDS sequence)	XM_005246961.3 , XM_011512183.2 , XM_006712852.3 , XM_017005285.1 , XM_005246961.4
Accession Version	XM_017005285.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860774.1
CDS	167..6247
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_017005285.1
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017005285.1

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIP12 ( XM_005246961.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247018.1 Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.

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CloneID	OHu36808
Clone ID Related Accession (Same CDS sequence)	XM_005246961.3 , XM_011512183.2 , XM_006712852.3 , XM_017005285.1 , XM_005246961.4
Accession Version	XM_005246961.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIP12 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005246961.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247018.1
CDS	180..6260
Translation	MSNRPNNNPGGSLRRSQRNTAGAQPQDDSIGGRSCSSSSAVIVPQPEDPDRANTSERQKTGQVPKKDNSRGVKRSASPDYNRTNSPSSAKKPKALQHTESPSETNKPHSKSKKRHLDQEQQLKSAQSPSTSKAHTRKSGATGGSRSQKRKRTESSCVKSGSGSESTGAEERSAKPTKLASKSATSAKAGCSTITDSSSAASTSSSSSAVASASSTVPPGARVKQGKDQNKARRSRSASSPSPRRSSREKEQSKTGGSSKFDWAARFSPKVSLPKTKLSLPGSSKSETSKPGPSGLQAKLASLRKSTKKRSESPPAELPSLRRSTRQKTTGSCASTSRRGSGLGKRGAAEARRQEKMADPESNQEAVNSSAARTDEAPQGAAASSSVAGAVGMTTSGESESDDSEMGRLQALLEARGLPPHLFGPLGPRMSQLFHRTIGSGASSKAQQLLQGLQASDESQQLQAVIEMCQLLVMGNEETLGGFPVKSVVPALITLLQMEHNFDIMNHACRALTYMMEALPRSSAVVVDAIPVFLEKLQVIQCIDVAEQALTALEMLSRRHSKAILQAGGLADCLLYLEFFSINAQRNALAIAANCCQSITPDEFHFVADSLPLLTQRLTHQDKKSVESTCLCFARLVDNFQHEENLLQQVASKDLLTNVQQLLVVTPPILSSGMFIMVVRMFSLMCSNCPTLAVQLMKQNIAETLHFLLCGASNGSCQEQIDLVPRSPQELYELTSLICELMPCLPKEGIFAVDTMLKKGNAQNTDGAIWQWRDDRGLWHPYNRIDSRIIEVAAHQVGEDEISLSTLGRVYTIDFNSMQQINEDTGTARAIQRKPNPLANSNTSGYSESKKDDARAQLMKEDPELAKSFIKTLFGVLYEVYSSSAGPAVRHKCLRAILRIIYFADAELLKDVLKNHAVSSHIASMLSSQDLKIVVGALQMAEILMQKLPDIFSVYFRREGVMHQVKHLAESESLLTSPPKACTNGSGSMGSTTSVSSGTATAATHAAADLGSPSLQHSRDDSLDLSPQGRLSDVLKRKRLPKRGPRRPKYSPPRDDDKVDNQAKSPTTTQSPKSSFLASLNPKTWGRLSTQSNSNNIEPARTAGGSGLARAASKDTISNNREKIKGWIKEQAHKFVERYFSSENMDGSNPALNVLQRLCAATEQLNLQVDGGAECLVEIRSIVSESDVSSFEIQHSGFVKQLLLYLTSKSEKDAVSREIRLKRFLHVFFSSPLPGEEPIGRVEPVGNAPLLALVHKMNNCLSQMEQFPVKVHDFPSGNGTGGSFSLNRGSQALKFFNTHQLKCQLQRHPDCANVKQWKGGPVKIDPLALVQAIERYLVVRGYGRVREDDEDSDDDGSDEEIDESLAAQFLNSGNVRHRLQFYIGEHLLPYNMTVYQAVRQFSIQAEDERESTDDESNPLGRAGIWTKTHTIWYKPVREDEESNKDCVGGKRGRAQTAPTKTSPRNAKKHDELWHDGVCPSVSNPLEVYLIPTPPENITFEDPSLDVILLLRVLHAISRYWYYLYDNAMCKEIIPTSEFINSKLTAKANRQLQDPLVIMTGNIPTWLTELGKTCPFFFPFDTRQMLFYVTAFDRDRAMQRLLDTNPEINQSDSQDSRVAPRLDRKKRTVNREELLKQAESVMQDLGSSRAMLEIQYENEVGTGLGPTLEFYALVSQELQRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS

Target ORF information:

RefSeq Version	XM_005246961.4
Organism	Homo sapiens(human)
Definition	Homo sapiens thyroid hormone receptor interactor 12 (TRIP12), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005246961.4

ORF Insert Sequence:

ATGTCCAACC GGCCTAATAA CAATCCAGGG GGGTCACTGC GACGTTCACA GAGGAACACT 
GCCGGGGCCC AACCACAAGA CGACTCAATA GGAGGAAGAA GCTGCAGTTC ATCATCTGCT 
GTGATAGTTC CACAACCAGA GGATCCAGAC AGAGCCAATA CTTCAGAAAG ACAAAAAACG 
GGGCAGGTGC CTAAGAAAGA CAATTCTCGA GGAGTGAAGC GCAGTGCTAG TCCAGACTAC 
AACAGGACCA ATTCTCCTAG CTCTGCAAAA AAACCAAAAG CACTTCAGCA TACTGAATCT 
CCCTCAGAAA CAAATAAGCC ACATAGTAAG TCAAAGAAGA GACATTTAGA CCAGGAGCAA 
CAACTGAAAT CTGCACAATC ACCATCAACA AGCAAGGCTC ATACCAGGAA GAGTGGGGCC 
ACTGGCGGTT CACGGAGTCA GAAAAGAAAA AGGACAGAGA GTTCTTGTGT AAAGAGTGGC 
TCCGGGTCTG AATCAACTGG TGCAGAAGAG AGATCTGCGA AACCTACCAA GCTGGCTTCA 
AAATCAGCCA CCTCAGCCAA AGCTGGGTGT AGCACCATCA CTGATTCTTC TTCTGCTGCC 
TCTACTTCCT CCTCGTCTTC TGCTGTAGCC TCGGCCTCCT CCACTGTACC ACCAGGTGCC 
AGAGTGAAAC AAGGAAAAGA TCAGAACAAG GCCAGGCGTT CCCGTTCAGC GTCCAGTCCC 
AGCCCCAGAA GAAGTAGCAG GGAAAAGGAA CAGAGTAAAA CTGGTGGCTC TTCAAAATTT 
GATTGGGCTG CTCGTTTCAG CCCTAAAGTT AGCCTTCCTA AAACAAAACT GTCTCTTCCA 
GGGTCTTCTA AGTCAGAGAC ATCAAAACCT GGACCTTCTG GATTACAGGC CAAATTAGCA 
AGTTTAAGAA AATCTACGAA GAAACGCAGT GAGTCTCCAC CTGCTGAGCT CCCCAGTTTG 
AGGCGGAGCA CACGCCAAAA GACCACGGGC TCCTGTGCTA GTACCAGTCG GCGAGGCTCT 
GGCCTGGGCA AAAGAGGAGC AGCTGAAGCT CGTCGACAGG AGAAAATGGC AGACCCTGAA 
AGCAACCAGG AGGCAGTAAA TTCTTCAGCT GCTCGGACAG ATGAAGCTCC CCAAGGAGCT 
GCAGCCTCTA GTTCTGTTGC AGGGGCTGTT GGCATGACCA CCTCTGGGGA GAGTGAATCA 
GATGATTCCG AGATGGGACG TTTGCAAGCT TTGTTAGAGG CAAGGGGTCT TCCCCCTCAC 
CTATTTGGTC CTCTTGGTCC TCGGATGTCA CAGCTTTTCC ATAGAACAAT TGGAAGTGGA 
GCTAGTTCTA AGGCCCAGCA GCTACTACAA GGATTGCAAG CCAGTGATGA AAGTCAACAG 
CTTCAGGCAG TTATTGAGAT GTGTCAGTTA CTGGTCATGG GAAATGAGGA GACACTGGGA 
GGGTTTCCTG TCAAGAGTGT TGTTCCAGCT TTGATTACGT TACTTCAGAT GGAGCACAAT 
TTTGATATTA TGAACCATGC TTGTCGAGCC TTAACATACA TGATGGAAGC ACTTCCTCGA 
TCTTCTGCTG TTGTAGTAGA TGCTATTCCT GTCTTTTTAG AAAAGCTGCA AGTTATTCAG 
TGTATTGATG TGGCAGAGCA GGCCTTGACT GCCTTGGAGA TGTTGTCACG GAGACATAGT 
AAAGCCATTC TACAGGCGGG TGGTTTGGCA GACTGCTTGC TGTACCTAGA ATTCTTCAGC 
ATAAATGCCC AAAGAAATGC ATTAGCAATT GCAGCTAATT GCTGCCAGAG TATCACGCCA 
GATGAATTTC ATTTTGTGGC AGATTCACTC CCATTGCTAA CCCAAAGGCT AACACATCAG 
GATAAAAAGT CAGTAGAAAG CACTTGCCTT TGTTTTGCAC GCCTAGTGGA CAACTTCCAG 
CATGAGGAGA ATTTACTCCA GCAGGTTGCT TCCAAAGATC TGCTTACAAA TGTTCAACAG 
CTGTTGGTAG TGACTCCACC CATTTTAAGT TCTGGGATGT TTATAATGGT GGTTCGCATG 
TTTTCTCTGA TGTGTTCCAA CTGTCCAACT TTAGCTGTTC AACTTATGAA ACAAAACATT 
GCAGAAACGC TTCACTTTCT CCTGTGTGGT GCCTCCAATG GAAGTTGTCA GGAACAGATT 
GATCTTGTTC CACGAAGCCC TCAAGAGTTG TATGAACTGA CATCTCTGAT TTGTGAACTT 
ATGCCATGTT TACCAAAAGA AGGCATTTTT GCAGTTGATA CCATGTTGAA GAAGGGAAAT 
GCACAGAACA CAGATGGTGC GATATGGCAG TGGCGTGATG ATCGGGGCCT CTGGCATCCA 
TATAACAGGA TTGACAGCCG GATCATTGAG GTAGCAGCCC ATCAGGTCGG TGAGGATGAG 
ATAAGCTTGT CCACTCTGGG ACGAGTTTAT ACTATTGATT TTAATTCTAT GCAGCAAATC 
AATGAGGACA CGGGAACAGC ACGTGCCATT CAGAGAAAAC CTAACCCGTT AGCCAATAGT 
AACACTAGTG GATATTCAGA GTCAAAGAAG GATGATGCTC GAGCACAGCT TATGAAAGAG 
GATCCGGAAC TGGCTAAGTC TTTTATTAAG ACATTATTTG GTGTTCTTTA TGAAGTGTAT 
AGTTCCTCAG CAGGACCTGC GGTCAGACAT AAGTGCCTTA GAGCAATTCT TAGGATAATT 
TATTTTGCGG ATGCTGAACT TCTGAAGGAT GTTCTGAAAA ATCATGCTGT TTCAAGTCAC 
ATTGCTTCCA TGCTGTCAAG CCAAGACCTG AAGATAGTAG TGGGAGCACT TCAGATGGCA 
GAAATTTTAA TGCAGAAGTT ACCTGATATT TTTAGTGTTT ACTTCAGAAG AGAAGGTGTA 
ATGCATCAAG TAAAACACTT AGCAGAATCA GAGTCTTTGT TGACAAGTCC ACCAAAGGCA 
TGTACGAATG GATCGGGATC CATGGGATCC ACAACTTCAG TCAGCAGTGG GACAGCCACA 
GCTGCCACTC ATGCTGCAGC TGACTTGGGA TCACCCAGCT TGCAGCACAG CAGGGATGAT 
TCTTTAGATC TCAGCCCTCA AGGTCGATTA AGTGATGTTC TAAAGAGAAA ACGACTGCCA 
AAACGAGGGC CAAGAAGGCC AAAGTACTCA CCTCCAAGAG ATGATGACAA AGTAGACAAT 
CAAGCTAAAA GCCCCACCAC TACTCAGTCA CCTAAATCTT CTTTCCTGGC AAGCTTGAAT 
CCAAAAACAT GGGGAAGGTT AAGTACACAG TCCAACAGCA ACAACATTGA GCCAGCACGG 
ACTGCGGGAG GTAGTGGCCT TGCCAGGGCT GCCTCAAAGG ATACCATCTC CAATAATAGA 
GAAAAAATTA AAGGTTGGAT TAAGGAGCAG GCACATAAAT TTGTAGAACG TTATTTCAGT 
TCTGAGAATA TGGATGGAAG CAACCCTGCA TTGAATGTCC TTCAGAGACT TTGTGCTGCA 
ACCGAACAAC TCAACCTCCA GGTGGATGGT GGAGCTGAGT GCCTTGTAGA AATCCGTAGC 
ATAGTCTCAG AGTCAGATGT TTCATCATTT GAAATCCAAC ATAGTGGATT TGTGAAGCAG 
CTGTTGCTTT ATTTGACATC TAAAAGTGAA AAGGATGCTG TGAGCAGAGA GATCAGATTA 
AAGCGATTTC TTCATGTATT TTTTTCTTCT CCACTTCCTG GAGAAGAGCC CATTGGAAGA 
GTGGAACCAG TGGGTAATGC ACCTTTGTTG GCATTAGTTC ACAAGATGAA CAACTGCCTC 
AGCCAGATGG AACAATTTCC AGTCAAAGTA CATGATTTCC CTAGTGGAAA TGGGACAGGA 
GGCAGCTTTT CTCTCAACAG AGGATCACAG GCTTTAAAAT TTTTCAACAC ACATCAATTA 
AAATGCCAGT TACAAAGGCA TCCAGACTGT GCAAATGTGA AGCAGTGGAA GGGTGGACCT 
GTCAAGATTG ACCCTCTGGC TTTGGTACAA GCCATCGAGA GATACCTTGT AGTTAGAGGG 
TATGGAAGAG TAAGAGAAGA TGATGAAGAC AGCGATGACG ATGGATCAGA TGAGGAAATA 
GATGAGTCTC TGGCTGCTCA GTTCCTAAAT TCAGGAAATG TAAGACACAG GCTGCAGTTT 
TATATTGGAG AACATTTGCT GCCGTATAAC ATGACTGTGT ATCAGGCAGT ACGGCAGTTT 
AGTATACAGG CTGAAGATGA AAGAGAATCC ACAGATGATG AGAGCAATCC TCTAGGCAGA 
GCTGGTATTT GGACAAAGAC TCATACAATA TGGTATAAAC CTGTGAGAGA GGATGAAGAA 
AGTAATAAAG ATTGTGTTGG TGGTAAAAGA GGAAGAGCCC AAACAGCTCC AACGAAAACT 
TCCCCTAGAA ATGCAAAAAA GCATGATGAG TTATGGCACG ATGGAGTGTG CCCATCAGTA 
TCAAATCCTT TAGAAGTTTA CCTCATTCCC ACACCACCTG AAAATATAAC ATTTGAAGAC 
CCGTCATTAG ATGTGATCCT TCTTTTAAGA GTTTTACATG CTATCAGTCG ATACTGGTAT 
TACTTGTATG ATAATGCAAT GTGCAAGGAA ATTATTCCAA CTAGTGAATT TATTAACAGT 
AAGTTAACAG CAAAAGCAAA TAGGCAACTT CAAGATCCTT TAGTAATCAT GACAGGAAAC 
ATCCCAACAT GGCTTACTGA GCTAGGAAAA ACCTGCCCAT TTTTCTTTCC TTTTGATACC 
CGGCAAATGC TTTTTTATGT AACTGCATTT GATCGGGACC GAGCAATGCA AAGATTACTT 
GATACCAACC CAGAAATCAA CCAGTCTGAT TCTCAAGATA GCAGAGTTGC ACCTAGATTG 
GATAGAAAAA AACGTACTGT GAACCGAGAG GAGCTGCTGA AACAGGCGGA GTCTGTGATG 
CAGGACCTCG GCAGCTCACG GGCCATGTTA GAAATCCAGT ATGAAAATGA GGTTGGTACA 
GGTCTTGGGC CTACACTGGA GTTTTATGCG CTTGTATCTC AGGAACTACA GAGAGCTGAC 
TTGGGTCTTT GGAGAGGTGA AGAAGTAACT CTTAGCAATC CAAAAGGGAG CCAAGAAGGG 
ACCAAGTATA TTCAAAACCT CCAGGGCCTG TTTGCGCTTC CCTTTGGTAG GACAGCAAAG 
CCAGCTCATA TCGCAAAGGT TAAGATGAAG TTTCGCTTCT TAGGAAAATT AATGGCCAAG 
GCTATCATGG ATTTCAGATT GGTGGACCTT CCCCTTGGCT TACCCTTTTA TAAATGGATG 
CTACGGCAAG AAACTTCACT GACATCACAC GATTTGTTTG ACATCGACCC AGTTGTAGCC 
AGATCAGTTT ATCACCTAGA AGACATTGTC AGACAGAAGA AAAGACTTGA ACAAGATAAA 
TCCCAGACCA AAGAGAGTCT ACAGTATGCA TTAGAAACCT TGACTATGAA TGGCTGCTCA 
GTTGAAGATC TAGGACTGGA TTTCACTCTG CCAGGGTTTC CCAATATCGA ACTGAAGAAA 
GGAGGGAAGG ATATACCAGT CACTATCCAC AATTTAGAGG AGTATCTAAG ACTGGTTATA 
TTCTGGGCAC TAAATGAAGG CGTTTCTAGG CAATTTGATT CGTTCAGAGA TGGATTTGAA 
TCAGTCTTCC CACTCAGTCA TCTTCAGTAC TTCTACCCGG AGGAACTGGA TCAGCTCCTT 
TGTGGCAGTA AAGCAGACAC TTGGGATGCA AAGACACTGA TGGAATGCTG TAGGCCTGAT 
CATGGTTATA CTCATGACAG TCGGGCTGTG AAGTTTTTGT TTGAGATTCT CAGTAGTTTT 
GATAATGAGC AGCAGAGGTT ATTTCTCCAG TTTGTGACTG GTAGCCCAAG ATTGCCTGTT 
GGAGGATTCC GGAGTTTGAA TCCACCTTTG ACAATTGTCC GAAAGACGTT TGAATCAACA 
GAAAACCCAG ATGACTTCTT GCCCTCTGTA ATGACTTGTG TGAACTATCT TAAGTTGCCG 
GACTATTCAA GCATTGAGAT AATGCGTGAA AAACTGTTGA TAGCAGCAAG AGAAGGGCAG 
CAGTCGTTCC ATCTTTCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Haploinsufficiency of the E3 ubiquitin-protein ligase gene TRIP12 causes intellectual disability with or without autism spectrum disorders, speech delay, and dysmorphic features.
Human genetics136(4)377-386(2017 Apr)
Zhang J,Gambin T,Yuan B,Szafranski P,Rosenfeld JA,Balwi MA,Alswaid A,Al-Gazali L,Shamsi AMA,Komara M,Ali BR,Roeder E,McAuley L,Roy DS,Manchester DK,Magoulas P,King LE,Hannig V,Bonneau D,Denomm?-Pichon AS,Charif M,Besnard T,B?zieau S,Cogn? B,Andrieux J,Zhu W,He W,Vetrini F,Ward PA,Cheung SW,Bi W,Eng CM,Lupski JR,Yang Y,Patel A,Lalani SR,Xia F,Stankiewicz P

TRIP12 as a mediator of human papillomavirus/p16-related radiation enhancement effects.
Oncogene36(6)820-828(2017 Feb)
Wang L,Zhang P,Molkentine DP,Chen C,Molkentine JM,Piao H,Raju U,Zhang J,Valdecanas DR,Tailor RC,Thames HD,Buchholz TA,Chen J,Ma L,Mason KA,Ang KK,Meyn RE,Skinner HD

Identification of new TRIP12 variants and detailed clinical evaluation of individuals with non-syndromic intellectual disability with or without autism.
Human genetics136(2)179-192(2017 Feb)
Bramswig NC,L?decke HJ,Pettersson M,Albrecht B,Bernier RA,Cremer K,Eichler EE,Falkenstein D,Gerdts J,Jansen S,Kuechler A,Kvarnung M,Lindstrand A,Nilsson D,Nordgren A,Pfundt R,Spruijt L,Surowy HM,de Vries BB,Wieland T,Engels H,Strom TM,Kleefstra T,Wieczorek D

Trip12 is an E3 ubiquitin ligase for USP7/HAUSP involved in the DNA damage response.
FEBS letters590(23)4213-4222(2016 Dec)
Liu X,Yang X,Li Y,Zhao S,Li C,Ma P,Mao B

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TRIP12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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