MED14 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED14
Entrez Gene ID	9282
Full Name	mediator complex subunit 14
Synonyms	CRSP150,CRSP2,CSRP,CXorf4,DRIP150,EXLM1,RGR1,TRAP170
General protein information	Preferred Names mediator complex subunit 14 Names mediator of RNA polymerase II transcription subunit 14 ARC150 CRSP complex subunit 2 RGR1 homolog activator-recruited cofactor 150 kDa component cofactor required for Sp1 transcriptional activation, subunit 2 (150kD) hRGR1 human homolog of yeast RGR1 thyroid hormone receptor-associated protein complex 170 kDa component thyroid hormone receptor-associated protein complex component TRAP170 transcriptional co-activator CRSP150 vitamin D receptor-interacting protein complex component DRIP150 vitamin D3 receptor-interacting protein complex 150 kDa component
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp11.4
Summary	The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation. [provided by RefSeq, Jul 2008].
Disorder MIM:	300182

mRNA and Protein(s)

mRNA	Protein	Name
XM_011544000.3	XP_011542302.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
NM_004229.3	NP_004220.2	mediator of RNA polymerase II transcription subunit 14
XM_011544000.2	XP_011542302.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_017029962.2	XP_016885451.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_005272701.3	XP_005272758.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_005272701.2	XP_005272758.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
XM_017029962.1	XP_016885451.1	mediator of RNA polymerase II transcription subunit 14 isoform X1
NM_004229.4	NP_004220.2	mediator of RNA polymerase II transcription subunit 14

Pathways from BioSystems

Homologies

	MED14	XP_001085867.1
Gallus gallus (chicken)	MED14	XP_416776.2
Xenopus tropicalis (tropical clawed frog)	med14	XP_002932839.1
Homo sapiens (human)	MED14	NP_004220.2
Bos taurus (cattle)	MED14	NP_001193960.1
Mus musculus (house mouse)	Med14	NP_001041673.1
Rattus norvegicus (Norway rat)	Med14	NP_001178656.1
Danio rerio (zebrafish)	med14	NP_997930.2
Anopheles gambiae (African malaria mosquito)	MED14_ANOGA	XP_315707.4
Drosophila melanogaster (fruit fly)	MED14	NP_612041.2
Pan troglodytes (chimpanzee)	MED14	XP_003317475.2
Canis lupus familiaris (dog)	MED14	XP_538001.2

Gene Ontology
Bibliography

Related articles in PubMed

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
Hicks JA, Li L, Matsui M, Chu Y, Volkov O, Johnson KC, Corey DR
Cell reports20(7)1543-1552(2017 Aug)

Homozygous MED25 mutation implicated in eye-intellectual disability syndrome.
Basel-Vanagaite L, Smirin-Yosef P, Essakow JL, Tzur S, Lagovsky I, Maya I, Pasmanik-Chor M, Yeheskel A, Konen O, Orenstein N, Weisz Hubshman M, Drasinover V, Magal N, Peretz Amit G, Zalzstein Y, Zeharia A, Shohat M, Straussberg R, Mont? D, Salmon-Divon M, Behar DM
Human genetics134(6)577-87(2015 Jun)

Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.
Cevher MA, Shi Y, Li D, Chait BT, Malik S, Roeder RG
Nature structural & molecular biology21(12)1028-34(2014 Dec)

RNA-Seq for the identification of novel Mediator transcripts in endothelial progenitor cells.
Rienzo M, Costa V, Scarpato M, Schiano C, Casamassimi A, Grimaldi V, Ciccodicola A, Napoli C
Gene547(1)98-105(2014 Aug)

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This results from a dramatically enhanced ability of MED14-containing complexes to associate with Pol II.
Title: Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.

VitD-mediated stimulation of GC anti-inflammatory affects human monocytes in a process involving GM-CSF and MED14
Title: Vitamin D enhances glucocorticoid action in human monocytes: involvement of granulocyte-macrophage colony-stimulating factor and mediator complex subunit 14.

Coactivator that enhances estrogen receptor alpha- and specificity protein (SP)-1-mediated transactivation in breast cancer cells.
Title: Coactivation of estrogen receptor alpha (ER alpha)/Sp1 by vitamin D receptor interacting protein 150 (DRIP150).

MED14 and MED1 are used by glucocorticoid receptor in a gene-specific manner, providing a mechanism for promoter selectivity by glucocorticoid receptor
Title: MED14 and MED1 differentially regulate target-specific gene activation by the glucocorticoid receptor.

Coactivation of ERalpha by DRIP150 in ZR-75 cells is NR box-independent and requires a novel sequence with putative alpha-helical structure.
Title: DRIP150 coactivation of estrogen receptor alpha in ZR-75 breast cancer cells is independent of LXXLL motifs.

The following MED14 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED14 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25793	NM_004229.4 Latest version!	Homo sapiens mediator complex subunit 14 (MED14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu54060	XM_005272701.3 Latest version!	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54060	XM_011544000.3 Latest version!	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54060	XM_017029962.2 Latest version!	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu54060	XM_011544000.2	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54060	XM_005272701.2	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu54060	XM_017029962.1	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu25793	NM_004229.3	Homo sapiens mediator complex subunit 14 (MED14), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED14 ( NM_004229.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004220.2 Homo sapiens mediator complex subunit 14 (MED14), mRNA.

Price & Availability↑

CloneID	OHu25793
Clone ID Related Accession (Same CDS sequence)	NM_004229.3 , NM_004229.4
Accession Version	NM_004229.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-09
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC098377.1 and AF304448.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2007 this sequence version replaced NM_004229.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC098377.1, AB006651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCGG CGGCGGGGGC 
AGCGGCGGAC CCCCGTCAGC CCCAGCCCCT CCTCCCCCGG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCTG CGGCTAGCCC GGGCTACCGG CTGAGCACCC TCATTGAATT TCTGCTGCAC 
CGGGCCTACT CGGAGCTTAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTGGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGCC AACTCTTCGT TCGATTATTA 
GCTTTAGTGA AATGGGCTAA TAATGCTGGC AAAGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT CCTGTTTGTG GACACTGCTG ATCGCCTGGC CTCGTTAGCT 
AGAGATGCTC TGGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCATATGC CATTGATGTA 
CTAACTACTG GATCTTACCC ACGGCTGCCA ACCTGCATTA GGGACAAAAT TATTCCTCCA 
GACCCAATTA CCAAAATTGA AAAACAAGCC ACACTCCATC AGCTGAATCA GATTCTTAGA 
CATCGGCTTG TAACCACAGA TCTTCCTCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGTGTTGA AGGAGAATTT GAAGCCACCT TGACTGTGAT GGGAGATGAC 
CCTGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGATAA GGAAACAGGA 
GATGGGCGAG CTTTGGTTCA TAGCATGCAA ATCAGCTTCA TCCATCAACT GGTGCAGTCT 
AGGCTCTTTG CTGATGAGAA ACCTCTTCAG GATATGTACA ACTGCCTACA TTCTTTCTGT 
TTATCACTTC AGTTAGAAGT GTTACATTCC CAAACTCTAA TGTTAATCCG AGAACGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTATCAT GCTGGAAAGT GCCTCTCCCT TTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACTGGA ACAGCATCTG TTCACAAAGT TACAATTAAA 
ATTGATGAGA ATGATGTCTC CAAGCCTTTA CAGATTTTTC ACGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATCGACC ACTTATCAAT AGAAAAACTC 
CTGATTGACA GTGTCCATGC AAGAGCTCAT CAGAAGCTCC AAGAACTGAA GGCCATTCTT 
AGAGGCTTCA ATGCCAATGA AAACTCTTCC ATAGAGACTG CACTCCCAGC TCTTGTTGTT 
CCCATCTTGG AGCCCTGTGG TAATTCAGAG TGTCTGCACA TTTTTGTAGA TTTACATTCT 
GGAATGTTTC AATTGATGCT TTATGGACTT GACCAGGCCA CTCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATCATA CCCTGGATTC AGCAACTTAA GTTTTGGCTT 
GGACAACAGC GTTGCAAGCA GTCTATAAAA CATCTGCCTA CGATAAGCAG TGAAACATTG 
CAGCTTTCCA ATTACTCAAC TCATCCTATT GGAAACCTTT CTAAGAATAA ACTGTTCATT 
AAACTTACCC GCCTTCCACA ATACTACATT GTTGTGGAGA TGTTGGAGGT TCCCAATAAA 
CCCACACAAC TGTCGTACAA GTACTACTTT ATGTCTGTGA ATGCTGCAGA TCGTGAAGAC 
AGCCCTGCAA TGGCATTGCT GCTGCAGCAG TTCAAGGAAA ACATTCAGGA CTTGGTTTTT 
CGTACAAAAA CCGGGAAACA GACCAGAACC AATGCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AATCCAAGAA AACAAAACGA GCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
TTAGCCCACT TCGTCGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
TTGTCCAATC TGGAGATTCC ACATCAAGGA GTGCAAGTGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TATTAAAAAT TCCTCCCTGT AAGGGTATAA CTGAGGAAAC CCAAAAGGCT 
CTGGACCGCT CTCTTCTTGA TTGCACTTTC CGATTACAAG GTAGAAATAA CCGCACTTGG 
GTAGCAGAGT TAGTGTTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGA 
CCATCCCGGC ACGTTTACCT GACATATGAA AATCTGTTGT CTGAGCCTGT TGGTGGTAGA 
AAGGTGGTTG AAATGTTTCT TAATGACTGG AATAGCATTG CACGATTATA TGAGTGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTT 
CGTGTTTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGTATCCAAT GGAATTCGAT CCATCAAAAA TTCCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACACCAA ATGTGGTTCA GTTATTACAG GTACTGTTTG ATACTCAGGC TCCATTAAAT 
GCCATCAACA AACTCCCCAC TGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAATACTGCC 
TACCAGTGCT TCTCCATTCT GCCACAGTCG TCCACCCACA TCAGACTGGC CTTCAGGAAC 
ATGTATTGCA TTGATATATA CTGCCGGAGT CGAGGTGTTG TGGCAATACG GGATGGTGCC 
TATAGTCTTT TTGATAACAG CAAACTAGTT GAAGGTTTCT ATCCTGCACC AGGACTAAAG 
ACGTTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC TATAGGAGGA GATATGATGG ATTCTTTAAT ATCGCAGCTC 
CAGCCACCAC CCCAGCAACA GCCATTTCCA AAGCAGCCAG GAACATCAGG TGCTTATCCT 
CTTACTTCAC CCCCTACATC TTATCATAGC ACAGTCAATC AGTCTCCCTC AATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCA 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACT CTTGACCCTA GTTCCCCATA CACTATGGTG 
TCACCAAGTG GACGAGCAGG GAACTGGCCA GGATCTCCTC AAGTGTCTGG CCCCTCACCA 
GCAGCACGCA TGCCTGGAAT GTCACCAGCC AACCCCTCAC TACATTCTCC GGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCTCAAACAA TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCTTGGGCG GCATCCATAC CTACCATCCT CACTCACAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACTCCAGGCC TTGTGCCCGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCACT TGAGAGATTC CTTGGATCAG TCATCATGAG ACGACATCTT 
CAAAGAATTA TTCAACAAGA AACGCTGCAG CTGATAAATT CTAATGAACC CGGAGTGATC 
ATGTTTAAGA CTGATGCACT GAAATGCAGA GTAGCTCTTA GTCCCAAAAC CAACCAAACG 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCACCAT TTAAAGCTAA TACGTTAATA 
GCCTTCACCA AGCTATTAGG AGCTCCTACA CACATCCTCA GGGATTGTGT GCACATTATG 
AAGCTGGAGC TGTTCCCTGA CCAAGCAACA CAGCTAAAAT GGAATGTTCA GTTTTGCCTG 
ACGATCCCTC CCAGCGCGCC GCCGATTGCA CCTCCTGGGA CGCCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAACTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCAAT CATTTATGAC ATGGCTTCAG GTACAACCCA GCAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAATCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004220.2
CDS	141..4505
Translation	MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	NM_004229.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004229.3

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCGG CGGCGGGGGC 
AGCGGCGGAC CCCCGTCAGC CCCAGCCCCT CCTCCCCCGG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCTG CGGCTAGCCC GGGCTACCGG CTGAGCACCC TCATTGAATT TCTGCTGCAC 
CGGGCCTACT CGGAGCTTAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTGGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGCC AACTCTTCGT TCGATTATTA 
GCTTTAGTGA AATGGGCTAA TAATGCTGGC AAAGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT CCTGTTTGTG GACACTGCTG ATCGCCTGGC CTCGTTAGCT 
AGAGATGCTC TGGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCATATGC CATTGATGTA 
CTAACTACTG GATCTTACCC ACGGCTGCCA ACCTGCATTA GGGACAAAAT TATTCCTCCA 
GACCCAATTA CCAAAATTGA AAAACAAGCC ACACTCCATC AGCTGAATCA GATTCTTAGA 
CATCGGCTTG TAACCACAGA TCTTCCTCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGTGTTGA AGGAGAATTT GAAGCCACCT TGACTGTGAT GGGAGATGAC 
CCTGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGATAA GGAAACAGGA 
GATGGGCGAG CTTTGGTTCA TAGCATGCAA ATCAGCTTCA TCCATCAACT GGTGCAGTCT 
AGGCTCTTTG CTGATGAGAA ACCTCTTCAG GATATGTACA ACTGCCTACA TTCTTTCTGT 
TTATCACTTC AGTTAGAAGT GTTACATTCC CAAACTCTAA TGTTAATCCG AGAACGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTATCAT GCTGGAAAGT GCCTCTCCCT TTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACTGGA ACAGCATCTG TTCACAAAGT TACAATTAAA 
ATTGATGAGA ATGATGTCTC CAAGCCTTTA CAGATTTTTC ACGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATCGACC ACTTATCAAT AGAAAAACTC 
CTGATTGACA GTGTCCATGC AAGAGCTCAT CAGAAGCTCC AAGAACTGAA GGCCATTCTT 
AGAGGCTTCA ATGCCAATGA AAACTCTTCC ATAGAGACTG CACTCCCAGC TCTTGTTGTT 
CCCATCTTGG AGCCCTGTGG TAATTCAGAG TGTCTGCACA TTTTTGTAGA TTTACATTCT 
GGAATGTTTC AATTGATGCT TTATGGACTT GACCAGGCCA CTCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATCATA CCCTGGATTC AGCAACTTAA GTTTTGGCTT 
GGACAACAGC GTTGCAAGCA GTCTATAAAA CATCTGCCTA CGATAAGCAG TGAAACATTG 
CAGCTTTCCA ATTACTCAAC TCATCCTATT GGAAACCTTT CTAAGAATAA ACTGTTCATT 
AAACTTACCC GCCTTCCACA ATACTACATT GTTGTGGAGA TGTTGGAGGT TCCCAATAAA 
CCCACACAAC TGTCGTACAA GTACTACTTT ATGTCTGTGA ATGCTGCAGA TCGTGAAGAC 
AGCCCTGCAA TGGCATTGCT GCTGCAGCAG TTCAAGGAAA ACATTCAGGA CTTGGTTTTT 
CGTACAAAAA CCGGGAAACA GACCAGAACC AATGCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AATCCAAGAA AACAAAACGA GCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
TTAGCCCACT TCGTCGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
TTGTCCAATC TGGAGATTCC ACATCAAGGA GTGCAAGTGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TATTAAAAAT TCCTCCCTGT AAGGGTATAA CTGAGGAAAC CCAAAAGGCT 
CTGGACCGCT CTCTTCTTGA TTGCACTTTC CGATTACAAG GTAGAAATAA CCGCACTTGG 
GTAGCAGAGT TAGTGTTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGA 
CCATCCCGGC ACGTTTACCT GACATATGAA AATCTGTTGT CTGAGCCTGT TGGTGGTAGA 
AAGGTGGTTG AAATGTTTCT TAATGACTGG AATAGCATTG CACGATTATA TGAGTGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTT 
CGTGTTTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGTATCCAAT GGAATTCGAT CCATCAAAAA TTCCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACACCAA ATGTGGTTCA GTTATTACAG GTACTGTTTG ATACTCAGGC TCCATTAAAT 
GCCATCAACA AACTCCCCAC TGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAATACTGCC 
TACCAGTGCT TCTCCATTCT GCCACAGTCG TCCACCCACA TCAGACTGGC CTTCAGGAAC 
ATGTATTGCA TTGATATATA CTGCCGGAGT CGAGGTGTTG TGGCAATACG GGATGGTGCC 
TATAGTCTTT TTGATAACAG CAAACTAGTT GAAGGTTTCT ATCCTGCACC AGGACTAAAG 
ACGTTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC TATAGGAGGA GATATGATGG ATTCTTTAAT ATCGCAGCTC 
CAGCCACCAC CCCAGCAACA GCCATTTCCA AAGCAGCCAG GAACATCAGG TGCTTATCCT 
CTTACTTCAC CCCCTACATC TTATCATAGC ACAGTCAATC AGTCTCCCTC AATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCA 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACT CTTGACCCTA GTTCCCCATA CACTATGGTG 
TCACCAAGTG GACGAGCAGG GAACTGGCCA GGATCTCCTC AAGTGTCTGG CCCCTCACCA 
GCAGCACGCA TGCCTGGAAT GTCACCAGCC AACCCCTCAC TACATTCTCC GGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCTCAAACAA TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCTTGGGCG GCATCCATAC CTACCATCCT CACTCACAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACTCCAGGCC TTGTGCCCGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCACT TGAGAGATTC CTTGGATCAG TCATCATGAG ACGACATCTT 
CAAAGAATTA TTCAACAAGA AACGCTGCAG CTGATAAATT CTAATGAACC CGGAGTGATC 
ATGTTTAAGA CTGATGCACT GAAATGCAGA GTAGCTCTTA GTCCCAAAAC CAACCAAACG 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCACCAT TTAAAGCTAA TACGTTAATA 
GCCTTCACCA AGCTATTAGG AGCTCCTACA CACATCCTCA GGGATTGTGT GCACATTATG 
AAGCTGGAGC TGTTCCCTGA CCAAGCAACA CAGCTAAAAT GGAATGTTCA GTTTTGCCTG 
ACGATCCCTC CCAGCGCGCC GCCGATTGCA CCTCCTGGGA CGCCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAACTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCAAT CATTTATGAC ATGGCTTCAG GTACAACCCA GCAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAATCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( NM_004229.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004220.2 Homo sapiens mediator complex subunit 14 (MED14), mRNA.

Price & Availability↑

CloneID	OHu25793
Clone ID Related Accession (Same CDS sequence)	NM_004229.3 , NM_004229.4
Accession Version	NM_004229.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC098377.1, AF304448.1 and AC093029.4. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004229.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB006651.1, AF304448.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000324817.6/ ENSP00000323720.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCGG CGGCGGGGGC 
AGCGGCGGAC CCCCGTCAGC CCCAGCCCCT CCTCCCCCGG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCTG CGGCTAGCCC GGGCTACCGG CTGAGCACCC TCATTGAATT TCTGCTGCAC 
CGGGCCTACT CGGAGCTTAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTGGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGCC AACTCTTCGT TCGATTATTA 
GCTTTAGTGA AATGGGCTAA TAATGCTGGC AAAGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT CCTGTTTGTG GACACTGCTG ATCGCCTGGC CTCGTTAGCT 
AGAGATGCTC TGGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCATATGC CATTGATGTA 
CTAACTACTG GATCTTACCC ACGGCTGCCA ACCTGCATTA GGGACAAAAT TATTCCTCCA 
GACCCAATTA CCAAAATTGA AAAACAAGCC ACACTCCATC AGCTGAATCA GATTCTTAGA 
CATCGGCTTG TAACCACAGA TCTTCCTCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGTGTTGA AGGAGAATTT GAAGCCACCT TGACTGTGAT GGGAGATGAC 
CCTGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGATAA GGAAACAGGA 
GATGGGCGAG CTTTGGTTCA TAGCATGCAA ATCAGCTTCA TCCATCAACT GGTGCAGTCT 
AGGCTCTTTG CTGATGAGAA ACCTCTTCAG GATATGTACA ACTGCCTACA TTCTTTCTGT 
TTATCACTTC AGTTAGAAGT GTTACATTCC CAAACTCTAA TGTTAATCCG AGAACGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTATCAT GCTGGAAAGT GCCTCTCCCT TTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACTGGA ACAGCATCTG TTCACAAAGT TACAATTAAA 
ATTGATGAGA ATGATGTCTC CAAGCCTTTA CAGATTTTTC ACGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATCGACC ACTTATCAAT AGAAAAACTC 
CTGATTGACA GTGTCCATGC AAGAGCTCAT CAGAAGCTCC AAGAACTGAA GGCCATTCTT 
AGAGGCTTCA ATGCCAATGA AAACTCTTCC ATAGAGACTG CACTCCCAGC TCTTGTTGTT 
CCCATCTTGG AGCCCTGTGG TAATTCAGAG TGTCTGCACA TTTTTGTAGA TTTACATTCT 
GGAATGTTTC AATTGATGCT TTATGGACTT GACCAGGCCA CTCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATCATA CCCTGGATTC AGCAACTTAA GTTTTGGCTT 
GGACAACAGC GTTGCAAGCA GTCTATAAAA CATCTGCCTA CGATAAGCAG TGAAACATTG 
CAGCTTTCCA ATTACTCAAC TCATCCTATT GGAAACCTTT CTAAGAATAA ACTGTTCATT 
AAACTTACCC GCCTTCCACA ATACTACATT GTTGTGGAGA TGTTGGAGGT TCCCAATAAA 
CCCACACAAC TGTCGTACAA GTACTACTTT ATGTCTGTGA ATGCTGCAGA TCGTGAAGAC 
AGCCCTGCAA TGGCATTGCT GCTGCAGCAG TTCAAGGAAA ACATTCAGGA CTTGGTTTTT 
CGTACAAAAA CCGGGAAACA GACCAGAACC AATGCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AATCCAAGAA AACAAAACGA GCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
TTAGCCCACT TCGTCGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
TTGTCCAATC TGGAGATTCC ACATCAAGGA GTGCAAGTGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TATTAAAAAT TCCTCCCTGT AAGGGTATAA CTGAGGAAAC CCAAAAGGCT 
CTGGACCGCT CTCTTCTTGA TTGCACTTTC CGATTACAAG GTAGAAATAA CCGCACTTGG 
GTAGCAGAGT TAGTGTTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGA 
CCATCCCGGC ACGTTTACCT GACATATGAA AATCTGTTGT CTGAGCCTGT TGGTGGTAGA 
AAGGTGGTTG AAATGTTTCT TAATGACTGG AATAGCATTG CACGATTATA TGAGTGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTT 
CGTGTTTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGTATCCAAT GGAATTCGAT CCATCAAAAA TTCCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACACCAA ATGTGGTTCA GTTATTACAG GTACTGTTTG ATACTCAGGC TCCATTAAAT 
GCCATCAACA AACTCCCCAC TGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAATACTGCC 
TACCAGTGCT TCTCCATTCT GCCACAGTCG TCCACCCACA TCAGACTGGC CTTCAGGAAC 
ATGTATTGCA TTGATATATA CTGCCGGAGT CGAGGTGTTG TGGCAATACG GGATGGTGCC 
TATAGTCTTT TTGATAACAG CAAACTAGTT GAAGGTTTCT ATCCTGCACC AGGACTAAAG 
ACGTTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC TATAGGAGGA GATATGATGG ATTCTTTAAT ATCGCAGCTC 
CAGCCACCAC CCCAGCAACA GCCATTTCCA AAGCAGCCAG GAACATCAGG TGCTTATCCT 
CTTACTTCAC CCCCTACATC TTATCATAGC ACAGTCAATC AGTCTCCCTC AATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCA 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACT CTTGACCCTA GTTCCCCATA CACTATGGTG 
TCACCAAGTG GACGAGCAGG GAACTGGCCA GGATCTCCTC AAGTGTCTGG CCCCTCACCA 
GCAGCACGCA TGCCTGGAAT GTCACCAGCC AACCCCTCAC TACATTCTCC GGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCTCAAACAA TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCTTGGGCG GCATCCATAC CTACCATCCT CACTCACAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACTCCAGGCC TTGTGCCCGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCACT TGAGAGATTC CTTGGATCAG TCATCATGAG ACGACATCTT 
CAAAGAATTA TTCAACAAGA AACGCTGCAG CTGATAAATT CTAATGAACC CGGAGTGATC 
ATGTTTAAGA CTGATGCACT GAAATGCAGA GTAGCTCTTA GTCCCAAAAC CAACCAAACG 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCACCAT TTAAAGCTAA TACGTTAATA 
GCCTTCACCA AGCTATTAGG AGCTCCTACA CACATCCTCA GGGATTGTGT GCACATTATG 
AAGCTGGAGC TGTTCCCTGA CCAAGCAACA CAGCTAAAAT GGAATGTTCA GTTTTGCCTG 
ACGATCCCTC CCAGCGCGCC GCCGATTGCA CCTCCTGGGA CGCCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAACTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCAAT CATTTATGAC ATGGCTTCAG GTACAACCCA GCAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAATCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004220.2
CDS	131..4495
Translation	MAPVQLENHQLVPPGGGGGGSGGPPSAPAPPPPGAAVAAAAAAAASPGYRLSTLIEFLLHRAYSELMVLTDLLPRKSDVERKIEIVQFASRTRQLFVRLLALVKWANNAGKVEKCAMISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	NM_004229.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004229.4

ORF Insert Sequence:

ATGGCCCCAG TGCAGCTGGA GAACCACCAG CTGGTCCCGC CCGGAGGCGG CGGCGGGGGC 
AGCGGCGGAC CCCCGTCAGC CCCAGCCCCT CCTCCCCCGG GAGCCGCCGT GGCGGCGGCC 
GCTGCAGCTG CGGCTAGCCC GGGCTACCGG CTGAGCACCC TCATTGAATT TCTGCTGCAC 
CGGGCCTACT CGGAGCTTAT GGTGTTGACG GACCTACTGC CAAGGAAATC TGATGTGGAA 
AGGAAAATAG AAATAGTGCA GTTTGCTAGC CGGACACGCC AACTCTTCGT TCGATTATTA 
GCTTTAGTGA AATGGGCTAA TAATGCTGGC AAAGTGGAAA AATGTGCGAT GATTTCAAGC 
TTTTTAGATC AGCAAGCCAT CCTGTTTGTG GACACTGCTG ATCGCCTGGC CTCGTTAGCT 
AGAGATGCTC TGGTCCATGC ACGCCTGCCT AGTTTTGCCA TCCCATATGC CATTGATGTA 
CTAACTACTG GATCTTACCC ACGGCTGCCA ACCTGCATTA GGGACAAAAT TATTCCTCCA 
GACCCAATTA CCAAAATTGA AAAACAAGCC ACACTCCATC AGCTGAATCA GATTCTTAGA 
CATCGGCTTG TAACCACAGA TCTTCCTCCT CAGTTAGCAA ATCTTACAGT TGCAAATGGC 
CGGGTGAAGT TTCGTGTTGA AGGAGAATTT GAAGCCACCT TGACTGTGAT GGGAGATGAC 
CCTGATGTTC CATGGCGTCT TCTCAAGCTA GAAATTCTAG TTGAGGATAA GGAAACAGGA 
GATGGGCGAG CTTTGGTTCA TAGCATGCAA ATCAGCTTCA TCCATCAACT GGTGCAGTCT 
AGGCTCTTTG CTGATGAGAA ACCTCTTCAG GATATGTACA ACTGCCTACA TTCTTTCTGT 
TTATCACTTC AGTTAGAAGT GTTACATTCC CAAACTCTAA TGTTAATCCG AGAACGGTGG 
GGAGACCTTG TGCAGGTGGA AAGGTATCAT GCTGGAAAGT GCCTCTCCCT TTCAGTTTGG 
AATCAACAGG TTCTTGGGAG AAAAACTGGA ACAGCATCTG TTCACAAAGT TACAATTAAA 
ATTGATGAGA ATGATGTCTC CAAGCCTTTA CAGATTTTTC ACGATCCTCC TTTGCCAGCT 
TCTGATTCCA AATTAGTAGA AAGAGCCATG AAGATCGACC ACTTATCAAT AGAAAAACTC 
CTGATTGACA GTGTCCATGC AAGAGCTCAT CAGAAGCTCC AAGAACTGAA GGCCATTCTT 
AGAGGCTTCA ATGCCAATGA AAACTCTTCC ATAGAGACTG CACTCCCAGC TCTTGTTGTT 
CCCATCTTGG AGCCCTGTGG TAATTCAGAG TGTCTGCACA TTTTTGTAGA TTTACATTCT 
GGAATGTTTC AATTGATGCT TTATGGACTT GACCAGGCCA CTCTGGATGA CATGGAGAAG 
TCTGTGAATG ATGATATGAA ACGAATCATA CCCTGGATTC AGCAACTTAA GTTTTGGCTT 
GGACAACAGC GTTGCAAGCA GTCTATAAAA CATCTGCCTA CGATAAGCAG TGAAACATTG 
CAGCTTTCCA ATTACTCAAC TCATCCTATT GGAAACCTTT CTAAGAATAA ACTGTTCATT 
AAACTTACCC GCCTTCCACA ATACTACATT GTTGTGGAGA TGTTGGAGGT TCCCAATAAA 
CCCACACAAC TGTCGTACAA GTACTACTTT ATGTCTGTGA ATGCTGCAGA TCGTGAAGAC 
AGCCCTGCAA TGGCATTGCT GCTGCAGCAG TTCAAGGAAA ACATTCAGGA CTTGGTTTTT 
CGTACAAAAA CCGGGAAACA GACCAGAACC AATGCCAAGC GCAAGTTGTC TGATGATCCA 
TGTCCAGTAG AATCCAAGAA AACAAAACGA GCAGGAGAAA TGTGTGCCTT CAATAAAGTT 
TTAGCCCACT TCGTCGCTAT GTGTGATACA AATATGCCAT TTGTAGGACT TCGGTTGGAG 
TTGTCCAATC TGGAGATTCC ACATCAAGGA GTGCAAGTGG AAGGTGATGG CTTCAGCCAT 
GCAATTCGCT TATTAAAAAT TCCTCCCTGT AAGGGTATAA CTGAGGAAAC CCAAAAGGCT 
CTGGACCGCT CTCTTCTTGA TTGCACTTTC CGATTACAAG GTAGAAATAA CCGCACTTGG 
GTAGCAGAGT TAGTGTTTGC AAATTGTCCA CTTAATGGCA CTTCTACTAG GGAGCAAGGA 
CCATCCCGGC ACGTTTACCT GACATATGAA AATCTGTTGT CTGAGCCTGT TGGTGGTAGA 
AAGGTGGTTG AAATGTTTCT TAATGACTGG AATAGCATTG CACGATTATA TGAGTGTGTG 
TTGGAATTTG CACGTTCTCT ACCAGACATA CCTGCTCATC TAAATATTTT CTCAGAAGTT 
CGTGTTTATA ATTACCGAAA ACTTATCTTG TGTTATGGAA CCACCAAGGG AAGCTCAATT 
AGTATCCAAT GGAATTCGAT CCATCAAAAA TTCCACATTT CTTTGGGAAC TGTTGGCCCA 
AACTCAGGTT GCAGTAACTG TCACAATACC ATTCTCCATC AGCTTCAAGA AATGTTCAAC 
AAAACACCAA ATGTGGTTCA GTTATTACAG GTACTGTTTG ATACTCAGGC TCCATTAAAT 
GCCATCAACA AACTCCCCAC TGTGCCGATG TTGGGCTTGA CCCAGAGAAC CAATACTGCC 
TACCAGTGCT TCTCCATTCT GCCACAGTCG TCCACCCACA TCAGACTGGC CTTCAGGAAC 
ATGTATTGCA TTGATATATA CTGCCGGAGT CGAGGTGTTG TGGCAATACG GGATGGTGCC 
TATAGTCTTT TTGATAACAG CAAACTAGTT GAAGGTTTCT ATCCTGCACC AGGACTAAAG 
ACGTTCCTGA ATATGTTTGT TGACAGCAAT CAGGATGCTC GAAGAAGGTC TGTAAATGAG 
GACGATAATC CCCCTTCTCC TATAGGAGGA GATATGATGG ATTCTTTAAT ATCGCAGCTC 
CAGCCACCAC CCCAGCAACA GCCATTTCCA AAGCAGCCAG GAACATCAGG TGCTTATCCT 
CTTACTTCAC CCCCTACATC TTATCATAGC ACAGTCAATC AGTCTCCCTC AATGATGCAC 
ACACAGTCTC CAGGAAATCT GCATGCTGCC AGCTCCCCCA GTGGGGCTTT GAGAGCCCCA 
TCACCAGCGT CATTTGTTCC AACTCCTCCC CCATCCTCGC ATGGAATCTC AATAGGACCA 
GGGGCCAGTT TTGCTAGTCC ACATGGAACT CTTGACCCTA GTTCCCCATA CACTATGGTG 
TCACCAAGTG GACGAGCAGG GAACTGGCCA GGATCTCCTC AAGTGTCTGG CCCCTCACCA 
GCAGCACGCA TGCCTGGAAT GTCACCAGCC AACCCCTCAC TACATTCTCC GGTTCCAGAT 
GCTTCTCATT CCCCTCGAGC TGGAACAAGT TCTCAAACAA TGCCAACAAA CATGCCTCCA 
CCTCGTAAAC TACCTCAGCG CTCTTGGGCG GCATCCATAC CTACCATCCT CACTCACAGT 
GCCTTGAACA TTTTACTGCT GCCCTCTCCA ACTCCAGGCC TTGTGCCCGG CCTGGCAGGT 
AGTTACCTTT GTTCTCCACT TGAGAGATTC CTTGGATCAG TCATCATGAG ACGACATCTT 
CAAAGAATTA TTCAACAAGA AACGCTGCAG CTGATAAATT CTAATGAACC CGGAGTGATC 
ATGTTTAAGA CTGATGCACT GAAATGCAGA GTAGCTCTTA GTCCCAAAAC CAACCAAACG 
CTTCAGCTAA AAGTGACACC TGAAAATGCA GGACAGTGGA AACCTGATGA ACTTCAAGTT 
TTGGAGAAAT TCTTTGAAAC AAGAGTTGCA GGACCACCAT TTAAAGCTAA TACGTTAATA 
GCCTTCACCA AGCTATTAGG AGCTCCTACA CACATCCTCA GGGATTGTGT GCACATTATG 
AAGCTGGAGC TGTTCCCTGA CCAAGCAACA CAGCTAAAAT GGAATGTTCA GTTTTGCCTG 
ACGATCCCTC CCAGCGCGCC GCCGATTGCA CCTCCTGGGA CGCCTGCTGT GGTGCTGAAA 
TCCAAAATGC TATTTTTTCT TCAACTAACT CAGAAAACAT CGGTCCCTCC CCAAGAACCT 
GTTAGTATTA TAGTTCCAAT CATTTATGAC ATGGCTTCAG GTACAACCCA GCAGGCAGAC 
ATTCCCAGAC AGCAGAACTC TTCTGTTGCT GCTCCCATGA TGGTCAGCAA CATTCTGAAG 
AGGTTTGCAG AGATGAATCC ACCACGACAA GGTGAATGCA CAATATTTGC AGCTGTTCGT 
GATTTAATGG CTAATCTTAC ACTGCCCCCT GGTGGGCGTC CATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_011544000.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542302.1 Homo sapiens mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu54060
Clone ID Related Accession (Same CDS sequence)	XM_011544000.3 , XM_011544000.2 , XM_017029962.2 , XM_005272701.3 , XM_005272701.2 , XM_017029962.1
Accession Version	XM_011544000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011544000.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542302.1
CDS	371..4387
Translation	MISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_011544000.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544000.3

ORF Insert Sequence:

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_011544000.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542302.1 Homo sapiens mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu54060
Clone ID Related Accession (Same CDS sequence)	XM_011544000.3 , XM_011544000.2 , XM_017029962.2 , XM_005272701.3 , XM_005272701.2 , XM_017029962.1
Accession Version	XM_011544000.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011544000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542302.1
CDS	371..4387
Translation	MISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_011544000.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011544000.2

ORF Insert Sequence:

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_017029962.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885451.1 Homo sapiens mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu54060
Clone ID Related Accession (Same CDS sequence)	XM_011544000.3 , XM_011544000.2 , XM_017029962.2 , XM_005272701.3 , XM_005272701.2 , XM_017029962.1
Accession Version	XM_017029962.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029962.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885451.1
CDS	364..4380
Translation	MISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_017029962.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029962.2

ORF Insert Sequence:

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_005272701.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272758.1 Homo sapiens mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu54060
Clone ID Related Accession (Same CDS sequence)	XM_011544000.3 , XM_011544000.2 , XM_017029962.2 , XM_005272701.3 , XM_005272701.2 , XM_017029962.1
Accession Version	XM_005272701.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005272701.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272758.1
CDS	399..4415
Translation	MISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_005272701.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272701.3

ORF Insert Sequence:

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_005272701.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005272758.1 Homo sapiens mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu54060
Clone ID Related Accession (Same CDS sequence)	XM_011544000.3 , XM_011544000.2 , XM_017029962.2 , XM_005272701.3 , XM_005272701.2 , XM_017029962.1
Accession Version	XM_005272701.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005272701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005272758.1
CDS	399..4415
Translation	MISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_005272701.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005272701.2

ORF Insert Sequence:

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED14 ( XM_017029962.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885451.1 Homo sapiens mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu54060
Clone ID Related Accession (Same CDS sequence)	XM_011544000.3 , XM_011544000.2 , XM_017029962.2 , XM_005272701.3 , XM_005272701.2 , XM_017029962.1
Accession Version	XM_017029962.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 14 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885451.1
CDS	364..4380
Translation	MISSFLDQQAILFVDTADRLASLARDALVHARLPSFAIPYAIDVLTTGSYPRLPTCIRDKIIPPDPITKIEKQATLHQLNQILRHRLVTTDLPPQLANLTVANGRVKFRVEGEFEATLTVMGDDPDVPWRLLKLEILVEDKETGDGRALVHSMQISFIHQLVQSRLFADEKPLQDMYNCLHSFCLSLQLEVLHSQTLMLIRERWGDLVQVERYHAGKCLSLSVWNQQVLGRKTGTASVHKVTIKIDENDVSKPLQIFHDPPLPASDSKLVERAMKIDHLSIEKLLIDSVHARAHQKLQELKAILRGFNANENSSIETALPALVVPILEPCGNSECLHIFVDLHSGMFQLMLYGLDQATLDDMEKSVNDDMKRIIPWIQQLKFWLGQQRCKQSIKHLPTISSETLQLSNYSTHPIGNLSKNKLFIKLTRLPQYYIVVEMLEVPNKPTQLSYKYYFMSVNAADREDSPAMALLLQQFKENIQDLVFRTKTGKQTRTNAKRKLSDDPCPVESKKTKRAGEMCAFNKVLAHFVAMCDTNMPFVGLRLELSNLEIPHQGVQVEGDGFSHAIRLLKIPPCKGITEETQKALDRSLLDCTFRLQGRNNRTWVAELVFANCPLNGTSTREQGPSRHVYLTYENLLSEPVGGRKVVEMFLNDWNSIARLYECVLEFARSLPDIPAHLNIFSEVRVYNYRKLILCYGTTKGSSISIQWNSIHQKFHISLGTVGPNSGCSNCHNTILHQLQEMFNKTPNVVQLLQVLFDTQAPLNAINKLPTVPMLGLTQRTNTAYQCFSILPQSSTHIRLAFRNMYCIDIYCRSRGVVAIRDGAYSLFDNSKLVEGFYPAPGLKTFLNMFVDSNQDARRRSVNEDDNPPSPIGGDMMDSLISQLQPPPQQQPFPKQPGTSGAYPLTSPPTSYHSTVNQSPSMMHTQSPGNLHAASSPSGALRAPSPASFVPTPPPSSHGISIGPGASFASPHGTLDPSSPYTMVSPSGRAGNWPGSPQVSGPSPAARMPGMSPANPSLHSPVPDASHSPRAGTSSQTMPTNMPPPRKLPQRSWAASIPTILTHSALNILLLPSPTPGLVPGLAGSYLCSPLERFLGSVIMRRHLQRIIQQETLQLINSNEPGVIMFKTDALKCRVALSPKTNQTLQLKVTPENAGQWKPDELQVLEKFFETRVAGPPFKANTLIAFTKLLGAPTHILRDCVHIMKLELFPDQATQLKWNVQFCLTIPPSAPPIAPPGTPAVVLKSKMLFFLQLTQKTSVPPQEPVSIIVPIIYDMASGTTQQADIPRQQNSSVAAPMMVSNILKRFAEMNPPRQGECTIFAAVRDLMANLTLPPGGRP

Target ORF information:

RefSeq Version	XM_017029962.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 14 (MED14), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029962.1

ORF Insert Sequence:

ATGATTTCAA GCTTTTTAGA TCAGCAAGCC ATCCTGTTTG TGGACACTGC TGATCGCCTG 
GCCTCGTTAG CTAGAGATGC TCTGGTCCAT GCACGCCTGC CTAGTTTTGC CATCCCATAT 
GCCATTGATG TACTAACTAC TGGATCTTAC CCACGGCTGC CAACCTGCAT TAGGGACAAA 
ATTATTCCTC CAGACCCAAT TACCAAAATT GAAAAACAAG CCACACTCCA TCAGCTGAAT 
CAGATTCTTA GACATCGGCT TGTAACCACA GATCTTCCTC CTCAGTTAGC AAATCTTACA 
GTTGCAAATG GCCGGGTGAA GTTTCGTGTT GAAGGAGAAT TTGAAGCCAC CTTGACTGTG 
ATGGGAGATG ACCCTGATGT TCCATGGCGT CTTCTCAAGC TAGAAATTCT AGTTGAGGAT 
AAGGAAACAG GAGATGGGCG AGCTTTGGTT CATAGCATGC AAATCAGCTT CATCCATCAA 
CTGGTGCAGT CTAGGCTCTT TGCTGATGAG AAACCTCTTC AGGATATGTA CAACTGCCTA 
CATTCTTTCT GTTTATCACT TCAGTTAGAA GTGTTACATT CCCAAACTCT AATGTTAATC 
CGAGAACGGT GGGGAGACCT TGTGCAGGTG GAAAGGTATC ATGCTGGAAA GTGCCTCTCC 
CTTTCAGTTT GGAATCAACA GGTTCTTGGG AGAAAAACTG GAACAGCATC TGTTCACAAA 
GTTACAATTA AAATTGATGA GAATGATGTC TCCAAGCCTT TACAGATTTT TCACGATCCT 
CCTTTGCCAG CTTCTGATTC CAAATTAGTA GAAAGAGCCA TGAAGATCGA CCACTTATCA 
ATAGAAAAAC TCCTGATTGA CAGTGTCCAT GCAAGAGCTC ATCAGAAGCT CCAAGAACTG 
AAGGCCATTC TTAGAGGCTT CAATGCCAAT GAAAACTCTT CCATAGAGAC TGCACTCCCA 
GCTCTTGTTG TTCCCATCTT GGAGCCCTGT GGTAATTCAG AGTGTCTGCA CATTTTTGTA 
GATTTACATT CTGGAATGTT TCAATTGATG CTTTATGGAC TTGACCAGGC CACTCTGGAT 
GACATGGAGA AGTCTGTGAA TGATGATATG AAACGAATCA TACCCTGGAT TCAGCAACTT 
AAGTTTTGGC TTGGACAACA GCGTTGCAAG CAGTCTATAA AACATCTGCC TACGATAAGC 
AGTGAAACAT TGCAGCTTTC CAATTACTCA ACTCATCCTA TTGGAAACCT TTCTAAGAAT 
AAACTGTTCA TTAAACTTAC CCGCCTTCCA CAATACTACA TTGTTGTGGA GATGTTGGAG 
GTTCCCAATA AACCCACACA ACTGTCGTAC AAGTACTACT TTATGTCTGT GAATGCTGCA 
GATCGTGAAG ACAGCCCTGC AATGGCATTG CTGCTGCAGC AGTTCAAGGA AAACATTCAG 
GACTTGGTTT TTCGTACAAA AACCGGGAAA CAGACCAGAA CCAATGCCAA GCGCAAGTTG 
TCTGATGATC CATGTCCAGT AGAATCCAAG AAAACAAAAC GAGCAGGAGA AATGTGTGCC 
TTCAATAAAG TTTTAGCCCA CTTCGTCGCT ATGTGTGATA CAAATATGCC ATTTGTAGGA 
CTTCGGTTGG AGTTGTCCAA TCTGGAGATT CCACATCAAG GAGTGCAAGT GGAAGGTGAT 
GGCTTCAGCC ATGCAATTCG CTTATTAAAA ATTCCTCCCT GTAAGGGTAT AACTGAGGAA 
ACCCAAAAGG CTCTGGACCG CTCTCTTCTT GATTGCACTT TCCGATTACA AGGTAGAAAT 
AACCGCACTT GGGTAGCAGA GTTAGTGTTT GCAAATTGTC CACTTAATGG CACTTCTACT 
AGGGAGCAAG GACCATCCCG GCACGTTTAC CTGACATATG AAAATCTGTT GTCTGAGCCT 
GTTGGTGGTA GAAAGGTGGT TGAAATGTTT CTTAATGACT GGAATAGCAT TGCACGATTA 
TATGAGTGTG TGTTGGAATT TGCACGTTCT CTACCAGACA TACCTGCTCA TCTAAATATT 
TTCTCAGAAG TTCGTGTTTA TAATTACCGA AAACTTATCT TGTGTTATGG AACCACCAAG 
GGAAGCTCAA TTAGTATCCA ATGGAATTCG ATCCATCAAA AATTCCACAT TTCTTTGGGA 
ACTGTTGGCC CAAACTCAGG TTGCAGTAAC TGTCACAATA CCATTCTCCA TCAGCTTCAA 
GAAATGTTCA ACAAAACACC AAATGTGGTT CAGTTATTAC AGGTACTGTT TGATACTCAG 
GCTCCATTAA ATGCCATCAA CAAACTCCCC ACTGTGCCGA TGTTGGGCTT GACCCAGAGA 
ACCAATACTG CCTACCAGTG CTTCTCCATT CTGCCACAGT CGTCCACCCA CATCAGACTG 
GCCTTCAGGA ACATGTATTG CATTGATATA TACTGCCGGA GTCGAGGTGT TGTGGCAATA 
CGGGATGGTG CCTATAGTCT TTTTGATAAC AGCAAACTAG TTGAAGGTTT CTATCCTGCA 
CCAGGACTAA AGACGTTCCT GAATATGTTT GTTGACAGCA ATCAGGATGC TCGAAGAAGG 
TCTGTAAATG AGGACGATAA TCCCCCTTCT CCTATAGGAG GAGATATGAT GGATTCTTTA 
ATATCGCAGC TCCAGCCACC ACCCCAGCAA CAGCCATTTC CAAAGCAGCC AGGAACATCA 
GGTGCTTATC CTCTTACTTC ACCCCCTACA TCTTATCATA GCACAGTCAA TCAGTCTCCC 
TCAATGATGC ACACACAGTC TCCAGGAAAT CTGCATGCTG CCAGCTCCCC CAGTGGGGCT 
TTGAGAGCCC CATCACCAGC GTCATTTGTT CCAACTCCTC CCCCATCCTC GCATGGAATC 
TCAATAGGAC CAGGGGCCAG TTTTGCTAGT CCACATGGAA CTCTTGACCC TAGTTCCCCA 
TACACTATGG TGTCACCAAG TGGACGAGCA GGGAACTGGC CAGGATCTCC TCAAGTGTCT 
GGCCCCTCAC CAGCAGCACG CATGCCTGGA ATGTCACCAG CCAACCCCTC ACTACATTCT 
CCGGTTCCAG ATGCTTCTCA TTCCCCTCGA GCTGGAACAA GTTCTCAAAC AATGCCAACA 
AACATGCCTC CACCTCGTAA ACTACCTCAG CGCTCTTGGG CGGCATCCAT ACCTACCATC 
CTCACTCACA GTGCCTTGAA CATTTTACTG CTGCCCTCTC CAACTCCAGG CCTTGTGCCC 
GGCCTGGCAG GTAGTTACCT TTGTTCTCCA CTTGAGAGAT TCCTTGGATC AGTCATCATG 
AGACGACATC TTCAAAGAAT TATTCAACAA GAAACGCTGC AGCTGATAAA TTCTAATGAA 
CCCGGAGTGA TCATGTTTAA GACTGATGCA CTGAAATGCA GAGTAGCTCT TAGTCCCAAA 
ACCAACCAAA CGCTTCAGCT AAAAGTGACA CCTGAAAATG CAGGACAGTG GAAACCTGAT 
GAACTTCAAG TTTTGGAGAA ATTCTTTGAA ACAAGAGTTG CAGGACCACC ATTTAAAGCT 
AATACGTTAA TAGCCTTCAC CAAGCTATTA GGAGCTCCTA CACACATCCT CAGGGATTGT 
GTGCACATTA TGAAGCTGGA GCTGTTCCCT GACCAAGCAA CACAGCTAAA ATGGAATGTT 
CAGTTTTGCC TGACGATCCC TCCCAGCGCG CCGCCGATTG CACCTCCTGG GACGCCTGCT 
GTGGTGCTGA AATCCAAAAT GCTATTTTTT CTTCAACTAA CTCAGAAAAC ATCGGTCCCT 
CCCCAAGAAC CTGTTAGTAT TATAGTTCCA ATCATTTATG ACATGGCTTC AGGTACAACC 
CAGCAGGCAG ACATTCCCAG ACAGCAGAAC TCTTCTGTTG CTGCTCCCAT GATGGTCAGC 
AACATTCTGA AGAGGTTTGC AGAGATGAAT CCACCACGAC AAGGTGAATG CACAATATTT 
GCAGCTGTTC GTGATTTAAT GGCTAATCTT ACACTGCCCC CTGGTGGGCG TCCATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human GW182 Paralogs Are the Central Organizers for RNA-Mediated Control of Transcription.
Cell reports20(7)1543-1552(2017 Aug)
Hicks JA,Li L,Matsui M,Chu Y,Volkov O,Johnson KC,Corey DR

Homozygous MED25 mutation implicated in eye-intellectual disability syndrome.
Human genetics134(6)577-87(2015 Jun)
Basel-Vanagaite L,Smirin-Yosef P,Essakow JL,Tzur S,Lagovsky I,Maya I,Pasmanik-Chor M,Yeheskel A,Konen O,Orenstein N,Weisz Hubshman M,Drasinover V,Magal N,Peretz Amit G,Zalzstein Y,Zeharia A,Shohat M,Straussberg R,Mont? D,Salmon-Divon M,Behar DM

Reconstitution of active human core Mediator complex reveals a critical role of the MED14 subunit.
Nature structural & molecular biology21(12)1028-34(2014 Dec)
Cevher MA,Shi Y,Li D,Chait BT,Malik S,Roeder RG

RNA-Seq for the identification of novel Mediator transcripts in endothelial progenitor cells.
Gene547(1)98-105(2014 Aug)
Rienzo M,Costa V,Scarpato M,Schiano C,Casamassimi A,Grimaldi V,Ciccodicola A,Napoli C

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MED14 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報