TIMM50 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TIMM50
Entrez Gene ID	92609
Full Name	translocase of inner mitochondrial membrane 50
Synonyms	MGCA9,TIM50,TIM50L
General protein information	Preferred Names translocase of inner mitochondrial membrane 50 Names mitochondrial import inner membrane translocase subunit TIM50 Tim50-like protein homolog of yeast Tim50
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.2
Summary	This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis. [provided by RefSeq, Jul 2016].
Disorder MIM:	607381
Disorder Html:	3-methylglutaconic aciduria, type IX, 617698 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001563.3	NP_001001563.1	mitochondrial import inner membrane translocase subunit TIM50 isoform 1
NM_001001563.1	NP_001001563.1	mitochondrial import inner membrane translocase subunit TIM50
NM_001001563.5	NP_001001563.2	mitochondrial import inner membrane translocase subunit TIM50 isoform 1
XM_011527491.3	XP_011525793.1	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
NM_001329559.1	NP_001316488.1	mitochondrial import inner membrane translocase subunit TIM50 isoform 2
XM_011527491.2	XP_011525793.1	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
NM_001329559.2	NP_001316488.1	mitochondrial import inner membrane translocase subunit TIM50 isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TIMM50	XP_001138592.1
Bos taurus (cattle)	TIMM50	NP_001030196.1
Mus musculus (house mouse)	Timm50	NP_079892.1
Rattus norvegicus (Norway rat)	LOC687295	XP_003748854.1
Xenopus tropicalis (tropical clawed frog)	timm50	NP_001120038.1
Gallus gallus (chicken)	TIMM50	XP_001233751.3
Danio rerio (zebrafish)	timm50	NP_956959.1
Drosophila melanogaster (fruit fly)	ttm50	NP_570027.1
Homo sapiens (human)	TIMM50	NP_001001563.1
Canis lupus familiaris (dog)	TIMM50	XP_533672.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001108	XP_322056.5

Gene Ontology
Bibliography

Related articles in PubMed

Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy.
Tang K, Zhao Y, Li H, Zhu M, Li W, Liu W, Zhu G, Xu D, Peng W, Xu YW
Journal of the American Heart Association6(4)(2017 Apr)

Phenotypic and Interaction Profiling of the Human Phosphatases Identifies Diverse Mitotic Regulators.
St-Denis N, Gupta GD, Lin ZY, Gonzalez-Badillo B, Veri AO, Knight JDR, Rajendran D, Couzens AL, Currie KW, Tkach JM, Cheung SWT, Pelletier L, Gingras AC
Cell reports17(9)2488-2501(2016 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Missense mutations in TIMM50 are likely manifesting by severe intellectual disability and epilepsy accompanied by 3-methylglutaconic aciduria and variable mitochondrial complex V deficiency.
Title: Mitochondrial epileptic encephalopathy, 3-methylglutaconic aciduria and variable complex V deficiency associated with TIMM50 mutations.

TIM50 has a role in regulating cell proliferation and apoptosis through decreasing mitochondrial membrane potential in breast cancer cell
Title: Loss of TIM50 suppresses proliferation and induces apoptosis in breast cancer.

the interaction of divalent metal ions with the intermembrane space domain of Tim50 (Tim50(IMS)) and the interaction of presequence peptides with Tim50(IMS) in presence of Ca(2+) ion was investigated.
Title: Interaction of divalent metal ions with human translocase of inner membrane of mitochondria Tim50.

The far-UV CD spectra of Tim50(IMS) in native and denatured states revealed that the protein has a significantly folded secondary structure consisted of alpha-helixes and beta-sheets.
Title: Expression and structural characterization of human translocase of inner membrane of mitochondria Tim50.

Tim50 regulates 3betaHSD2 expression and activity, representing a new role for translocases in steroidogenesis.
Title: Inner mitochondrial translocase Tim50 interacts with 3?-hydroxysteroid dehydrogenase type 2 to regulate adrenal and gonadal steroidogenesis.

The following TIMM50 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TIMM50 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30150	NM_001001563.3 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu74497	XM_011527491.3 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu101264	NM_001329559.2 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu107792	NM_001001563.5 Latest version!	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu30150	NM_001001563.1	Homo sapiens translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) (TIMM50), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu74497	XM_011527491.2	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu101264	NM_001329559.1	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TIMM50 ( NM_001001563.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001563.1 Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30150
Clone ID Related Accession (Same CDS sequence)	NM_001001563.3 , NM_001001563.1
Accession Version	NM_001001563.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI856163.1, BC121146.1, BC050082.1, BM463473.1, BU540967.1, AK098197.1, AC011500.7 and BC014674.1. This sequence is a reference standard in the RefSeqGene project. On Jul 19, 2016 this sequence version replaced NM_001001563.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.101377.1, SRR1660805.177954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCAG CTTTATCTCT GGGCAATAAG TGTGATCCCT TCCTTCGCTG CGTCCTTTGC 
AGGGGCGGTG GCGCTCTGCA AGGCCCAAGG GGGCGTGGTC CAGATGACTT TGAATCCCAG 
TTGTCGCCCC CAGGGTCAGC CAGGAGACTA GTCAGAAGCA AACGCGCCTG CGGCAATCCG 
CCCGATGCCT TTGGTCTCTC TCGGGCTTCC GTCCACCCGC CCCTCCCCCG CGTTTCCATT 
GGCTGTAGCT CCGGCCCGGG GCGGGCGAAG AGGGAGCGAG TGGGCGGGGC CGCGTGGCGT 
CAGCGCAAGA TGGCGGCCTC GGCAGCGGTG TTCTCGCGCT TGCGAAGCGG GCTCCGGCTC 
GGCTCGCGGG GACTGTGCAC GAGGTTGGCG ACGCCGCCCC GCCGGGCCCC AGATCAGGCC 
GCAGAGATCG GGAGCCGCGG GAGCACTAAG GCGCAAGGGC CACAGCAGCA GCCGGGCTCA 
GAGGGTCCCA GCTATGCCAA AAAAGTTGCG CTCTGGCTTG CTGGGCTGCT TGGAGCTGGT 
GGGACTGTGA GCGTCGTCTA TATCTTTGGA AACAACCCGG TGGACGAAAA TGGTGCCAAG 
ATTCCTGATG AGTTCGACAA TGATCCAATT CTGGTACAGC AGTTGCGCCG GACATACAAA 
TATTTCAAAG ATTATAGACA GATGATCATC GAGCCCACCA GCCCTTGCCT TCTCCCAGAC 
CCTCTGCAGG AACCGTACTA CCAGCCACCC TACACGCTCG TTTTGGAGCT CACCGGCGTC 
CTCTTGCATC CTGAGTGGTC GCTGGCCACT GGCTGGAGGT TTAAGAAGCG CCCAGGCATC 
GAGACCTTGT TCCAGCAGCT TGCCCCTTTA TATGAAATTG TCATCTTTAC GTCAGAGACT 
GGCATGACTG CGTTTCCACT CATTGATAGT GTGGACCCCC ATGGCTTCAT CTCCTACCGC 
CTATTCCGGG ACGCCACAAG ATACATGGAT GGACACCATG TAAAGGATAT TTCATGTCTG 
AATCGGGACC CAGCTCGAGT AGTAGTTGTG GACTGCAAGA AGGAAGCCTT CCGCCTGCAG 
CCCTATAACG GCGTTGCCCT GCGGCCCTGG GACGGCAACT CTGATGACCG GGTCTTGTTG 
GATCTGTCTG CCTTCCTCAA GACCATTGCA CTGAATGGTG TGGAGGACGT GCGAACCGTG 
CTGGAGCACT ATGCCCTGGA GGATGACCCG CTGGCGGCTT TCAAACAGCG GCAAAGCCGG 
CTAGAGCAGG AGGAGCAGCA GCGCCTGGCC GAGCTCTCCA AGTCCAACAA GCAGAACCTC 
TTCCTTGGCT CCCTCACCAG CCGCTTGTGG CCTCGCTCCA AACAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001563.1
CDS	134..1504
Translation	MASALSLGNKCDPFLRCVLCRGGGALQGPRGRGPDDFESQLSPPGSARRLVRSKRACGNPPDAFGLSRASVHPPLPRVSIGCSSGPGRAKRERVGGAAWRQRKMAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	NM_001001563.3
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001563.3

ORF Insert Sequence:

ATGGCCTCAG CTTTATCTCT GGGCAATAAG TGTGATCCCT TCCTTCGCTG CGTCCTTTGC 
AGGGGCGGTG GCGCTCTGCA AGGCCCAAGG GGGCGTGGTC CAGATGACTT TGAATCCCAG 
TTGTCGCCCC CAGGGTCAGC CAGGAGACTA GTCAGAAGCA AACGCGCCTG CGGCAATCCG 
CCCGATGCCT TTGGTCTCTC TCGGGCTTCC GTCCACCCGC CCCTCCCCCG CGTTTCCATT 
GGCTGTAGCT CCGGCCCGGG GCGGGCGAAG AGGGAGCGAG TGGGCGGGGC CGCGTGGCGT 
CAGCGCAAGA TGGCGGCCTC GGCAGCGGTG TTCTCGCGCT TGCGAAGCGG GCTCCGGCTC 
GGCTCGCGGG GACTGTGCAC GAGGTTGGCG ACGCCGCCCC GCCGGGCCCC AGATCAGGCC 
GCAGAGATCG GGAGCCGCGG GAGCACTAAG GCGCAAGGGC CACAGCAGCA GCCGGGCTCA 
GAGGGTCCCA GCTATGCCAA AAAAGTTGCG CTCTGGCTTG CTGGGCTGCT TGGAGCTGGT 
GGGACTGTGA GCGTCGTCTA TATCTTTGGA AACAACCCGG TGGACGAAAA TGGTGCCAAG 
ATTCCTGATG AGTTCGACAA TGATCCAATT CTGGTACAGC AGTTGCGCCG GACATACAAA 
TATTTCAAAG ATTATAGACA GATGATCATC GAGCCCACCA GCCCTTGCCT TCTCCCAGAC 
CCTCTGCAGG AACCGTACTA CCAGCCACCC TACACGCTCG TTTTGGAGCT CACCGGCGTC 
CTCTTGCATC CTGAGTGGTC GCTGGCCACT GGCTGGAGGT TTAAGAAGCG CCCAGGCATC 
GAGACCTTGT TCCAGCAGCT TGCCCCTTTA TATGAAATTG TCATCTTTAC GTCAGAGACT 
GGCATGACTG CGTTTCCACT CATTGATAGT GTGGACCCCC ATGGCTTCAT CTCCTACCGC 
CTATTCCGGG ACGCCACAAG ATACATGGAT GGACACCATG TAAAGGATAT TTCATGTCTG 
AATCGGGACC CAGCTCGAGT AGTAGTTGTG GACTGCAAGA AGGAAGCCTT CCGCCTGCAG 
CCCTATAACG GCGTTGCCCT GCGGCCCTGG GACGGCAACT CTGATGACCG GGTCTTGTTG 
GATCTGTCTG CCTTCCTCAA GACCATTGCA CTGAATGGTG TGGAGGACGT GCGAACCGTG 
CTGGAGCACT ATGCCCTGGA GGATGACCCG CTGGCGGCTT TCAAACAGCG GCAAAGCCGG 
CTAGAGCAGG AGGAGCAGCA GCGCCTGGCC GAGCTCTCCA AGTCCAACAA GCAGAACCTC 
TTCCTTGGCT CCCTCACCAG CCGCTTGTGG CCTCGCTCCA AACAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM50 ( NM_001001563.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001563.1 Homo sapiens translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) (TIMM50), mRNA.

Price & Availability↑

CloneID	OHu30150
Clone ID Related Accession (Same CDS sequence)	NM_001001563.3 , NM_001001563.1
Accession Version	NM_001001563.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	mitochondrial import inner membrane translocase subunit TIM50
Comment

ATGGCCTCAG CTTTATCTCT GGGCAATAAG TGTGATCCCT TCCTTCGCTG CGTCCTTTGC 
AGGGGCGGTG GCGCTCTGCA AGGCCCAAGG GGGCGTGGTC CAGATGACTT TGAATCCCAG 
TTGTCGCCCC CAGGGTCAGC CAGGAGACTA GTCAGAAGCA AACGCGCCTG CGGCAATCCG 
CCCGATGCCT TTGGTCTCTC TCGGGCTTCC GTCCACCCGC CCCTCCCCCG CGTTTCCATT 
GGCTGTAGCT CCGGCCCGGG GCGGGCGAAG AGGGAGCGAG TGGGCGGGGC CGCGTGGCGT 
CAGCGCAAGA TGGCGGCCTC GGCAGCGGTG TTCTCGCGCT TGCGAAGCGG GCTCCGGCTC 
GGCTCGCGGG GACTGTGCAC GAGGTTGGCG ACGCCGCCCC GCCGGGCCCC AGATCAGGCC 
GCAGAGATCG GGAGCCGCGG GAGCACTAAG GCGCAAGGGC CACAGCAGCA GCCGGGCTCA 
GAGGGTCCCA GCTATGCCAA AAAAGTTGCG CTCTGGCTTG CTGGGCTGCT TGGAGCTGGT 
GGGACTGTGA GCGTCGTCTA TATCTTTGGA AACAACCCGG TGGACGAAAA TGGTGCCAAG 
ATTCCTGATG AGTTCGACAA TGATCCAATT CTGGTACAGC AGTTGCGCCG GACATACAAA 
TATTTCAAAG ATTATAGACA GATGATCATC GAGCCCACCA GCCCTTGCCT TCTCCCAGAC 
CCTCTGCAGG AACCGTACTA CCAGCCACCC TACACGCTCG TTTTGGAGCT CACCGGCGTC 
CTCTTGCATC CTGAGTGGTC GCTGGCCACT GGCTGGAGGT TTAAGAAGCG CCCAGGCATC 
GAGACCTTGT TCCAGCAGCT TGCCCCTTTA TATGAAATTG TCATCTTTAC GTCAGAGACT 
GGCATGACTG CGTTTCCACT CATTGATAGT GTGGACCCCC ATGGCTTCAT CTCCTACCGC 
CTATTCCGGG ACGCCACAAG ATACATGGAT GGACACCATG TAAAGGATAT TTCATGTCTG 
AATCGGGACC CAGCTCGAGT AGTAGTTGTG GACTGCAAGA AGGAAGCCTT CCGCCTGCAG 
CCCTATAACG GCGTTGCCCT GCGGCCCTGG GACGGCAACT CTGATGACCG GGTCTTGTTG 
GATCTGTCTG CCTTCCTCAA GACCATTGCA CTGAATGGTG TGGAGGACGT GCGAACCGTG 
CTGGAGCACT ATGCCCTGGA GGATGACCCG CTGGCGGCTT TCAAACAGCG GCAAAGCCGG 
CTAGAGCAGG AGGAGCAGCA GCGCCTGGCC GAGCTCTCCA AGTCCAACAA GCAGAACCTC 
TTCCTTGGCT CCCTCACCAG CCGCTTGTGG CCTCGCTCCA AACAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_001001563.1

Translation

MASALSLGNKCDPFLRCVLCRGGGALQGPRGRGPDDFESQLSPPGSARRLVRSKRACGNPPDAFGLSRASVHPPLPRVSIGCSSGPGRAKRERVGGAAWRQRKMAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	NM_001001563.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 homolog (S. cerevisiae) (TIMM50), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001563.1

ORF Insert Sequence:

ATGGCCTCAG CTTTATCTCT GGGCAATAAG TGTGATCCCT TCCTTCGCTG CGTCCTTTGC 
AGGGGCGGTG GCGCTCTGCA AGGCCCAAGG GGGCGTGGTC CAGATGACTT TGAATCCCAG 
TTGTCGCCCC CAGGGTCAGC CAGGAGACTA GTCAGAAGCA AACGCGCCTG CGGCAATCCG 
CCCGATGCCT TTGGTCTCTC TCGGGCTTCC GTCCACCCGC CCCTCCCCCG CGTTTCCATT 
GGCTGTAGCT CCGGCCCGGG GCGGGCGAAG AGGGAGCGAG TGGGCGGGGC CGCGTGGCGT 
CAGCGCAAGA TGGCGGCCTC GGCAGCGGTG TTCTCGCGCT TGCGAAGCGG GCTCCGGCTC 
GGCTCGCGGG GACTGTGCAC GAGGTTGGCG ACGCCGCCCC GCCGGGCCCC AGATCAGGCC 
GCAGAGATCG GGAGCCGCGG GAGCACTAAG GCGCAAGGGC CACAGCAGCA GCCGGGCTCA 
GAGGGTCCCA GCTATGCCAA AAAAGTTGCG CTCTGGCTTG CTGGGCTGCT TGGAGCTGGT 
GGGACTGTGA GCGTCGTCTA TATCTTTGGA AACAACCCGG TGGACGAAAA TGGTGCCAAG 
ATTCCTGATG AGTTCGACAA TGATCCAATT CTGGTACAGC AGTTGCGCCG GACATACAAA 
TATTTCAAAG ATTATAGACA GATGATCATC GAGCCCACCA GCCCTTGCCT TCTCCCAGAC 
CCTCTGCAGG AACCGTACTA CCAGCCACCC TACACGCTCG TTTTGGAGCT CACCGGCGTC 
CTCTTGCATC CTGAGTGGTC GCTGGCCACT GGCTGGAGGT TTAAGAAGCG CCCAGGCATC 
GAGACCTTGT TCCAGCAGCT TGCCCCTTTA TATGAAATTG TCATCTTTAC GTCAGAGACT 
GGCATGACTG CGTTTCCACT CATTGATAGT GTGGACCCCC ATGGCTTCAT CTCCTACCGC 
CTATTCCGGG ACGCCACAAG ATACATGGAT GGACACCATG TAAAGGATAT TTCATGTCTG 
AATCGGGACC CAGCTCGAGT AGTAGTTGTG GACTGCAAGA AGGAAGCCTT CCGCCTGCAG 
CCCTATAACG GCGTTGCCCT GCGGCCCTGG GACGGCAACT CTGATGACCG GGTCTTGTTG 
GATCTGTCTG CCTTCCTCAA GACCATTGCA CTGAATGGTG TGGAGGACGT GCGAACCGTG 
CTGGAGCACT ATGCCCTGGA GGATGACCCG CTGGCGGCTT TCAAACAGCG GCAAAGCCGG 
CTAGAGCAGG AGGAGCAGCA GCGCCTGGCC GAGCTCTCCA AGTCCAACAA GCAGAACCTC 
TTCCTTGGCT CCCTCACCAG CCGCTTGTGG CCTCGCTCCA AACAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM50 ( XM_011527491.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525793.1 Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu74497
Clone ID Related Accession (Same CDS sequence)	XM_011527491.3 , XM_011527491.2
Accession Version	XM_011527491.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011527491.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGTC CCTTCCCTGG AGTTACGGAC ATATCGCCGC CCCTTGGGGG TTCCGGGACG 
CCTCACCTCA GGGCCGCAGA GATCGGGAGC CGCGGGAGCA CTAAGGCGCA AGGGCCACAG 
CAGCAGCCGG GCTCAGAGGG TCCCAGCTAT GCCAAAAAAG TTGCGCTCTG GCTTGCTGGG 
CTGCTTGGAG CTGGTGGGAC TGTGAGCGTC GTCTATATCT TTGGAAACAA CCCGGTGGAC 
GAAAATGGTG CCAAGATTCC TGATGAGTTC GACAATGATC CAATTCTGGT ACAGCAGTTG 
CGCCGGACAT ACAAATATTT CAAAGATTAT AGACAGATGA TCATCGAGCC CACCAGCCCT 
TGCCTTCTCC CAGACCCTCT GCAGGAACCG TACTACCAGC CACCCTACAC GCTCGTTTTG 
GAGCTCACCG GCGTCCTCTT GCATCCTGAG TGGTCGCTGG CCACTGGCTG GAGGTTTAAG 
AAGCGCCCAG GCATCGAGAC CTTGTTCCAG CAGCTTGCCC CTTTATATGA AATTGTCATC 
TTTACGTCAG AGACTGGCAT GACTGCGTTT CCACTCATTG ATAGTGTGGA CCCCCATGGC 
TTCATCTCCT ACCGCCTATT CCGGGACGCC ACAAGATACA TGGATGGACA CCATGTAAAG 
GATATTTCAT GTCTGAATCG GGACCCAGCT CGAGTAGTAG TTGTGGACTG CAAGAAGGAA 
GCCTTCCGCC TGCAGCCCTA TAACGGCGTT GCCCTGCGGC CCTGGGACGG CAACTCTGAT 
GACCGGGTCT TGTTGGATCT GTCTGCCTTC CTCAAGACCA TTGCACTGAA TGGTGTGGAG 
GACGTGCGAA CCGTGCTGGA GCACTATGCC CTGGAGGATG ACCCGCTGGC GGCTTTCAAA 
CAGCGGCAAA GCCGGCTAGA GCAGGAGGAG CAGCAGCGCC TGGCCGAGCT CTCCAAGTCC 
AACAAGCAGA ACCTCTTCCT TGGCTCCCTC ACCAGCCGCT TGTGGCCTCG CTCCAAACAG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525793.1
CDS	35..1060
Translation	MWGPFPGVTDISPPLGGSGTPHLRAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	XM_011527491.3
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527491.3

ORF Insert Sequence:

ATGTGGGGTC CCTTCCCTGG AGTTACGGAC ATATCGCCGC CCCTTGGGGG TTCCGGGACG 
CCTCACCTCA GGGCCGCAGA GATCGGGAGC CGCGGGAGCA CTAAGGCGCA AGGGCCACAG 
CAGCAGCCGG GCTCAGAGGG TCCCAGCTAT GCCAAAAAAG TTGCGCTCTG GCTTGCTGGG 
CTGCTTGGAG CTGGTGGGAC TGTGAGCGTC GTCTATATCT TTGGAAACAA CCCGGTGGAC 
GAAAATGGTG CCAAGATTCC TGATGAGTTC GACAATGATC CAATTCTGGT ACAGCAGTTG 
CGCCGGACAT ACAAATATTT CAAAGATTAT AGACAGATGA TCATCGAGCC CACCAGCCCT 
TGCCTTCTCC CAGACCCTCT GCAGGAACCG TACTACCAGC CACCCTACAC GCTCGTTTTG 
GAGCTCACCG GCGTCCTCTT GCATCCTGAG TGGTCGCTGG CCACTGGCTG GAGGTTTAAG 
AAGCGCCCAG GCATCGAGAC CTTGTTCCAG CAGCTTGCCC CTTTATATGA AATTGTCATC 
TTTACGTCAG AGACTGGCAT GACTGCGTTT CCACTCATTG ATAGTGTGGA CCCCCATGGC 
TTCATCTCCT ACCGCCTATT CCGGGACGCC ACAAGATACA TGGATGGACA CCATGTAAAG 
GATATTTCAT GTCTGAATCG GGACCCAGCT CGAGTAGTAG TTGTGGACTG CAAGAAGGAA 
GCCTTCCGCC TGCAGCCCTA TAACGGCGTT GCCCTGCGGC CCTGGGACGG CAACTCTGAT 
GACCGGGTCT TGTTGGATCT GTCTGCCTTC CTCAAGACCA TTGCACTGAA TGGTGTGGAG 
GACGTGCGAA CCGTGCTGGA GCACTATGCC CTGGAGGATG ACCCGCTGGC GGCTTTCAAA 
CAGCGGCAAA GCCGGCTAGA GCAGGAGGAG CAGCAGCGCC TGGCCGAGCT CTCCAAGTCC 
AACAAGCAGA ACCTCTTCCT TGGCTCCCTC ACCAGCCGCT TGTGGCCTCG CTCCAAACAG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM50 ( XM_011527491.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525793.1 Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu74497
Clone ID Related Accession (Same CDS sequence)	XM_011527491.3 , XM_011527491.2
Accession Version	XM_011527491.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGTC CCTTCCCTGG AGTTACGGAC ATATCGCCGC CCCTTGGGGG TTCCGGGACG 
CCTCACCTCA GGGCCGCAGA GATCGGGAGC CGCGGGAGCA CTAAGGCGCA AGGGCCACAG 
CAGCAGCCGG GCTCAGAGGG TCCCAGCTAT GCCAAAAAAG TTGCGCTCTG GCTTGCTGGG 
CTGCTTGGAG CTGGTGGGAC TGTGAGCGTC GTCTATATCT TTGGAAACAA CCCGGTGGAC 
GAAAATGGTG CCAAGATTCC TGATGAGTTC GACAATGATC CAATTCTGGT ACAGCAGTTG 
CGCCGGACAT ACAAATATTT CAAAGATTAT AGACAGATGA TCATCGAGCC CACCAGCCCT 
TGCCTTCTCC CAGACCCTCT GCAGGAACCG TACTACCAGC CACCCTACAC GCTCGTTTTG 
GAGCTCACCG GCGTCCTCTT GCATCCTGAG TGGTCGCTGG CCACTGGCTG GAGGTTTAAG 
AAGCGCCCAG GCATCGAGAC CTTGTTCCAG CAGCTTGCCC CTTTATATGA AATTGTCATC 
TTTACGTCAG AGACTGGCAT GACTGCGTTT CCACTCATTG ATAGTGTGGA CCCCCATGGC 
TTCATCTCCT ACCGCCTATT CCGGGACGCC ACAAGATACA TGGATGGACA CCATGTAAAG 
GATATTTCAT GTCTGAATCG GGACCCAGCT CGAGTAGTAG TTGTGGACTG CAAGAAGGAA 
GCCTTCCGCC TGCAGCCCTA TAACGGCGTT GCCCTGCGGC CCTGGGACGG CAACTCTGAT 
GACCGGGTCT TGTTGGATCT GTCTGCCTTC CTCAAGACCA TTGCACTGAA TGGTGTGGAG 
GACGTGCGAA CCGTGCTGGA GCACTATGCC CTGGAGGATG ACCCGCTGGC GGCTTTCAAA 
CAGCGGCAAA GCCGGCTAGA GCAGGAGGAG CAGCAGCGCC TGGCCGAGCT CTCCAAGTCC 
AACAAGCAGA ACCTCTTCCT TGGCTCCCTC ACCAGCCGCT TGTGGCCTCG CTCCAAACAG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525793.1
CDS	22..1047
Translation	MWGPFPGVTDISPPLGGSGTPHLRAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	XM_011527491.2
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527491.2

ORF Insert Sequence:

ATGTGGGGTC CCTTCCCTGG AGTTACGGAC ATATCGCCGC CCCTTGGGGG TTCCGGGACG 
CCTCACCTCA GGGCCGCAGA GATCGGGAGC CGCGGGAGCA CTAAGGCGCA AGGGCCACAG 
CAGCAGCCGG GCTCAGAGGG TCCCAGCTAT GCCAAAAAAG TTGCGCTCTG GCTTGCTGGG 
CTGCTTGGAG CTGGTGGGAC TGTGAGCGTC GTCTATATCT TTGGAAACAA CCCGGTGGAC 
GAAAATGGTG CCAAGATTCC TGATGAGTTC GACAATGATC CAATTCTGGT ACAGCAGTTG 
CGCCGGACAT ACAAATATTT CAAAGATTAT AGACAGATGA TCATCGAGCC CACCAGCCCT 
TGCCTTCTCC CAGACCCTCT GCAGGAACCG TACTACCAGC CACCCTACAC GCTCGTTTTG 
GAGCTCACCG GCGTCCTCTT GCATCCTGAG TGGTCGCTGG CCACTGGCTG GAGGTTTAAG 
AAGCGCCCAG GCATCGAGAC CTTGTTCCAG CAGCTTGCCC CTTTATATGA AATTGTCATC 
TTTACGTCAG AGACTGGCAT GACTGCGTTT CCACTCATTG ATAGTGTGGA CCCCCATGGC 
TTCATCTCCT ACCGCCTATT CCGGGACGCC ACAAGATACA TGGATGGACA CCATGTAAAG 
GATATTTCAT GTCTGAATCG GGACCCAGCT CGAGTAGTAG TTGTGGACTG CAAGAAGGAA 
GCCTTCCGCC TGCAGCCCTA TAACGGCGTT GCCCTGCGGC CCTGGGACGG CAACTCTGAT 
GACCGGGTCT TGTTGGATCT GTCTGCCTTC CTCAAGACCA TTGCACTGAA TGGTGTGGAG 
GACGTGCGAA CCGTGCTGGA GCACTATGCC CTGGAGGATG ACCCGCTGGC GGCTTTCAAA 
CAGCGGCAAA GCCGGCTAGA GCAGGAGGAG CAGCAGCGCC TGGCCGAGCT CTCCAAGTCC 
AACAAGCAGA ACCTCTTCCT TGGCTCCCTC ACCAGCCGCT TGTGGCCTCG CTCCAAACAG 
CCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM50 ( NM_001329559.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316488.1 Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101264
Clone ID Related Accession (Same CDS sequence)	NM_001329559.1 , NM_001329559.2
Accession Version	NM_001329559.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-28
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI856163.1, BC121146.1, BC050082.1, AC011500.7 and BC014674.1. On Jul 19, 2016 this sequence version replaced XM_017027472.1. Transcript Variant: This variant (2) differs in the 5' UTR, uses a downstream translation start site and lacks an alternate in-frame exon in its 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC121146.1, AY444561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGTGCCAA GATTCCTGAT GAGTTCGACA ATGATGATCA TCGAGCCCAC CAGCCCTTGC 
CTTCTCCCAG ACCCTCTGCA GGAACCGTAC TACCAGCCAC CCTACACGCT CGTTTTGGAG 
CTCACCGGCG TCCTCTTGCA TCCTGAGTGG TCGCTGGCCA CTGGCTGGAG GTTTAAGAAG 
CGCCCAGGCA TCGAGACCTT GTTCCAGCAG CTTGCCCCTT TATATGAAAT TGTCATCTTT 
ACGTCAGAGA CTGGCATGAC TGCGTTTCCA CTCATTGATA GTGTGGACCC CCATGGCTTC 
ATCTCCTACC GCCTATTCCG GGACGCCACA AGATACATGG ATGGACACCA TGTAAAGGAT 
ATTTCATGTC TGAATCGGGA CCCAGCTCGA GTAGTAGTTG TGGACTGCAA GAAGGAAGCC 
TTCCGCCTGC AGCCCTATAA CGGCGTTGCC CTGCGGCCCT GGGACGGCAA CTCTGATGAC 
CGGGTCTTGT TGGATCTGTC TGCCTTCCTC AAGACCATTG CACTGAATGG TGTGGAGGAC 
GTGCGAACCG TGCTGGAGCA CTATGCCCTG GAGGATGACC CGCTGGCGGC TTTCAAACAG 
CGGCAAAGCC GGCTAGAGCA GGAGGAGCAG CAGCGCCTGG CCGAGCTCTC CAAGTCCAAC 
AAGCAGAACC TCTTCCTTGG CTCCCTCACC AGCCGCTTGT GGCCTCGCTC CAAACAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316488.1
CDS	723..1445
Translation	MVPRFLMSSTMMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	NM_001329559.1
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329559.1

ORF Insert Sequence:

ATGGTGCCAA GATTCCTGAT GAGTTCGACA ATGATGATCA TCGAGCCCAC CAGCCCTTGC 
CTTCTCCCAG ACCCTCTGCA GGAACCGTAC TACCAGCCAC CCTACACGCT CGTTTTGGAG 
CTCACCGGCG TCCTCTTGCA TCCTGAGTGG TCGCTGGCCA CTGGCTGGAG GTTTAAGAAG 
CGCCCAGGCA TCGAGACCTT GTTCCAGCAG CTTGCCCCTT TATATGAAAT TGTCATCTTT 
ACGTCAGAGA CTGGCATGAC TGCGTTTCCA CTCATTGATA GTGTGGACCC CCATGGCTTC 
ATCTCCTACC GCCTATTCCG GGACGCCACA AGATACATGG ATGGACACCA TGTAAAGGAT 
ATTTCATGTC TGAATCGGGA CCCAGCTCGA GTAGTAGTTG TGGACTGCAA GAAGGAAGCC 
TTCCGCCTGC AGCCCTATAA CGGCGTTGCC CTGCGGCCCT GGGACGGCAA CTCTGATGAC 
CGGGTCTTGT TGGATCTGTC TGCCTTCCTC AAGACCATTG CACTGAATGG TGTGGAGGAC 
GTGCGAACCG TGCTGGAGCA CTATGCCCTG GAGGATGACC CGCTGGCGGC TTTCAAACAG 
CGGCAAAGCC GGCTAGAGCA GGAGGAGCAG CAGCGCCTGG CCGAGCTCTC CAAGTCCAAC 
AAGCAGAACC TCTTCCTTGG CTCCCTCACC AGCCGCTTGT GGCCTCGCTC CAAACAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM50 ( NM_001329559.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316488.1 Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101264
Clone ID Related Accession (Same CDS sequence)	NM_001329559.1 , NM_001329559.2
Accession Version	NM_001329559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC121146.1, BC050082.1, AC011500.7 and BC014674.1. On May 31, 2019 this sequence version replaced NM_001329559.1. Transcript Variant: This variant (2) differs in the 5' UTR, uses a downstream translation start site and lacks an alternate in-frame exon in its 5' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC121146.1, AY444561.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END##

ATGGTGCCAA GATTCCTGAT GAGTTCGACA ATGATGATCA TCGAGCCCAC CAGCCCTTGC 
CTTCTCCCAG ACCCTCTGCA GGAACCGTAC TACCAGCCAC CCTACACGCT CGTTTTGGAG 
CTCACCGGCG TCCTCTTGCA TCCTGAGTGG TCGCTGGCCA CTGGCTGGAG GTTTAAGAAG 
CGCCCAGGCA TCGAGACCTT GTTCCAGCAG CTTGCCCCTT TATATGAAAT TGTCATCTTT 
ACGTCAGAGA CTGGCATGAC TGCGTTTCCA CTCATTGATA GTGTGGACCC CCATGGCTTC 
ATCTCCTACC GCCTATTCCG GGACGCCACA AGATACATGG ATGGACACCA TGTAAAGGAT 
ATTTCATGTC TGAATCGGGA CCCAGCTCGA GTAGTAGTTG TGGACTGCAA GAAGGAAGCC 
TTCCGCCTGC AGCCCTATAA CGGCGTTGCC CTGCGGCCCT GGGACGGCAA CTCTGATGAC 
CGGGTCTTGT TGGATCTGTC TGCCTTCCTC AAGACCATTG CACTGAATGG TGTGGAGGAC 
GTGCGAACCG TGCTGGAGCA CTATGCCCTG GAGGATGACC CGCTGGCGGC TTTCAAACAG 
CGGCAAAGCC GGCTAGAGCA GGAGGAGCAG CAGCGCCTGG CCGAGCTCTC CAAGTCCAAC 
AAGCAGAACC TCTTCCTTGG CTCCCTCACC AGCCGCTTGT GGCCTCGCTC CAAACAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316488.1
CDS	297..1019
Translation	MVPRFLMSSTMMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	NM_001329559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329559.2

ORF Insert Sequence:

ATGGTGCCAA GATTCCTGAT GAGTTCGACA ATGATGATCA TCGAGCCCAC CAGCCCTTGC 
CTTCTCCCAG ACCCTCTGCA GGAACCGTAC TACCAGCCAC CCTACACGCT CGTTTTGGAG 
CTCACCGGCG TCCTCTTGCA TCCTGAGTGG TCGCTGGCCA CTGGCTGGAG GTTTAAGAAG 
CGCCCAGGCA TCGAGACCTT GTTCCAGCAG CTTGCCCCTT TATATGAAAT TGTCATCTTT 
ACGTCAGAGA CTGGCATGAC TGCGTTTCCA CTCATTGATA GTGTGGACCC CCATGGCTTC 
ATCTCCTACC GCCTATTCCG GGACGCCACA AGATACATGG ATGGACACCA TGTAAAGGAT 
ATTTCATGTC TGAATCGGGA CCCAGCTCGA GTAGTAGTTG TGGACTGCAA GAAGGAAGCC 
TTCCGCCTGC AGCCCTATAA CGGCGTTGCC CTGCGGCCCT GGGACGGCAA CTCTGATGAC 
CGGGTCTTGT TGGATCTGTC TGCCTTCCTC AAGACCATTG CACTGAATGG TGTGGAGGAC 
GTGCGAACCG TGCTGGAGCA CTATGCCCTG GAGGATGACC CGCTGGCGGC TTTCAAACAG 
CGGCAAAGCC GGCTAGAGCA GGAGGAGCAG CAGCGCCTGG CCGAGCTCTC CAAGTCCAAC 
AAGCAGAACC TCTTCCTTGG CTCCCTCACC AGCCGCTTGT GGCCTCGCTC CAAACAGCCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TIMM50 ( NM_001001563.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001563.2 Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107792
Clone ID Related Accession (Same CDS sequence)	NM_001001563.5
Accession Version	NM_001001563.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	mitochondrial import inner membrane translocase subunit TIM50 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI856163.1, BC050082.1, BM463473.1, BU540967.1, AK098197.1, AC011500.7 and BC014674.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001001563.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.264753.1, SRR1163658.101377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000607714.6/ ENSP00000475531.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGCCT CGGCAGCGGT GTTCTCGCGC TTGCGAAGCG GGCTCCGGCT CGGCTCGCGG 
GGACTGTGCA CGAGGTTGGC GACGCCGCCC CGCCGGGCCC CAGATCAGGC CGCAGAGATC 
GGGAGCCGCG GGAGCACTAA GGCGCAAGGG CCACAGCAGC AGCCGGGCTC AGAGGGTCCC 
AGCTATGCCA AAAAAGTTGC GCTCTGGCTT GCTGGGCTGC TTGGAGCTGG TGGGACTGTG 
AGCGTCGTCT ATATCTTTGG AAACAACCCG GTGGACGAAA ATGGTGCCAA GATTCCTGAT 
GAGTTCGACA ATGATCCAAT TCTGGTACAG CAGTTGCGCC GGACATACAA ATATTTCAAA 
GATTATAGAC AGATGATCAT CGAGCCCACC AGCCCTTGCC TTCTCCCAGA CCCTCTGCAG 
GAACCGTACT ACCAGCCACC CTACACGCTC GTTTTGGAGC TCACCGGCGT CCTCTTGCAT 
CCTGAGTGGT CGCTGGCCAC TGGCTGGAGG TTTAAGAAGC GCCCAGGCAT CGAGACCTTG 
TTCCAGCAGC TTGCCCCTTT ATATGAAATT GTCATCTTTA CGTCAGAGAC TGGCATGACT 
GCGTTTCCAC TCATTGATAG TGTGGACCCC CATGGCTTCA TCTCCTACCG CCTATTCCGG 
GACGCCACAA GATACATGGA TGGACACCAT GTAAAGGATA TTTCATGTCT GAATCGGGAC 
CCAGCTCGAG TAGTAGTTGT GGACTGCAAG AAGGAAGCCT TCCGCCTGCA GCCCTATAAC 
GGCGTTGCCC TGCGGCCCTG GGACGGCAAC TCTGATGACC GGGTCTTGTT GGATCTGTCT 
GCCTTCCTCA AGACCATTGC ACTGAATGGT GTGGAGGACG TGCGAACCGT GCTGGAGCAC 
TATGCCCTGG AGGATGACCC GCTGGCGGCT TTCAAACAGC GGCAAAGCCG GCTAGAGCAG 
GAGGAGCAGC AGCGCCTGGC CGAGCTCTCC AAGTCCAACA AGCAGAACCT CTTCCTTGGC 
TCCCTCACCA GCCGCTTGTG GCCTCGCTCC AAACAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001563.2
CDS	17..1078
Translation	MAASAAVFSRLRSGLRLGSRGLCTRLATPPRRAPDQAAEIGSRGSTKAQGPQQQPGSEGPSYAKKVALWLAGLLGAGGTVSVVYIFGNNPVDENGAKIPDEFDNDPILVQQLRRTYKYFKDYRQMIIEPTSPCLLPDPLQEPYYQPPYTLVLELTGVLLHPEWSLATGWRFKKRPGIETLFQQLAPLYEIVIFTSETGMTAFPLIDSVDPHGFISYRLFRDATRYMDGHHVKDISCLNRDPARVVVVDCKKEAFRLQPYNGVALRPWDGNSDDRVLLDLSAFLKTIALNGVEDVRTVLEHYALEDDPLAAFKQRQSRLEQEEQQRLAELSKSNKQNLFLGSLTSRLWPRSKQP

Target ORF information:

RefSeq Version	NM_001001563.5
Organism	Homo sapiens(human)
Definition	Homo sapiens translocase of inner mitochondrial membrane 50 (TIMM50), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001563.5

ORF Insert Sequence:

ATGGCGGCCT CGGCAGCGGT GTTCTCGCGC TTGCGAAGCG GGCTCCGGCT CGGCTCGCGG 
GGACTGTGCA CGAGGTTGGC GACGCCGCCC CGCCGGGCCC CAGATCAGGC CGCAGAGATC 
GGGAGCCGCG GGAGCACTAA GGCGCAAGGG CCACAGCAGC AGCCGGGCTC AGAGGGTCCC 
AGCTATGCCA AAAAAGTTGC GCTCTGGCTT GCTGGGCTGC TTGGAGCTGG TGGGACTGTG 
AGCGTCGTCT ATATCTTTGG AAACAACCCG GTGGACGAAA ATGGTGCCAA GATTCCTGAT 
GAGTTCGACA ATGATCCAAT TCTGGTACAG CAGTTGCGCC GGACATACAA ATATTTCAAA 
GATTATAGAC AGATGATCAT CGAGCCCACC AGCCCTTGCC TTCTCCCAGA CCCTCTGCAG 
GAACCGTACT ACCAGCCACC CTACACGCTC GTTTTGGAGC TCACCGGCGT CCTCTTGCAT 
CCTGAGTGGT CGCTGGCCAC TGGCTGGAGG TTTAAGAAGC GCCCAGGCAT CGAGACCTTG 
TTCCAGCAGC TTGCCCCTTT ATATGAAATT GTCATCTTTA CGTCAGAGAC TGGCATGACT 
GCGTTTCCAC TCATTGATAG TGTGGACCCC CATGGCTTCA TCTCCTACCG CCTATTCCGG 
GACGCCACAA GATACATGGA TGGACACCAT GTAAAGGATA TTTCATGTCT GAATCGGGAC 
CCAGCTCGAG TAGTAGTTGT GGACTGCAAG AAGGAAGCCT TCCGCCTGCA GCCCTATAAC 
GGCGTTGCCC TGCGGCCCTG GGACGGCAAC TCTGATGACC GGGTCTTGTT GGATCTGTCT 
GCCTTCCTCA AGACCATTGC ACTGAATGGT GTGGAGGACG TGCGAACCGT GCTGGAGCAC 
TATGCCCTGG AGGATGACCC GCTGGCGGCT TTCAAACAGC GGCAAAGCCG GCTAGAGCAG 
GAGGAGCAGC AGCGCCTGGC CGAGCTCTCC AAGTCCAACA AGCAGAACCT CTTCCTTGGC 
TCCCTCACCA GCCGCTTGTG GCCTCGCTCC AAACAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Translocase of Inner Membrane 50 Functions as a Novel Protective Regulator of Pathological Cardiac Hypertrophy.
Journal of the American Heart Association6(4)(2017 Apr)
Tang K,Zhao Y,Li H,Zhu M,Li W,Liu W,Zhu G,Xu D,Peng W,Xu YW

Phenotypic and Interaction Profiling of the Human Phosphatases Identifies Diverse Mitotic Regulators.
Cell reports17(9)2488-2501(2016 11)
St-Denis N,Gupta GD,Lin ZY,Gonzalez-Badillo B,Veri AO,Knight JDR,Rajendran D,Couzens AL,Currie KW,Tkach JM,Cheung SWT,Pelletier L,Gingras AC

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TIMM50 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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