MFHAS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MFHAS1
Entrez Gene ID	9258
Full Name	malignant fibrous histiocytoma amplified sequence 1
Synonyms	LRRC65,MASL1,ROCO4
General protein information	Preferred Names malignant fibrous histiocytoma amplified sequence 1 Names malignant fibrous histiocytoma-amplified sequence 1 MFH-amplified sequences with leucine-rich tandem repeats 1 leucine rich repeat containing 65 malignant fibrous histiocytoma-amplified sequence with leucine-rich tandem repeats 1 malignant fibrous histiocytoma-amplified sequences with leucine-rich tandem repeats 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8p23.1
Summary	Identified in a human 8p amplicon, this gene is a potential oncogene whose expression is enhanced in some malignant fibrous histiocytomas (MFH). The primary structure of its product includes an ATP/GTP-binding site, three leucine zipper domains, and a leucine-rich tandem repeat, which are structural or functional elements for interactions among proteins related to the cell cycle, and which suggest that overexpression might be oncogenic with respect to MFH. [provided by RefSeq, Jul 2008].
Disorder MIM:	605352
Disorder Html:	Malignant fibrous histiocytoma (2)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004225.2	NP_004216.2	malignant fibrous histiocytoma-amplified sequence 1
XM_011543852.2	XP_011542154.1	malignant fibrous histiocytoma-amplified sequence 1 isoform X1
XM_011543852.3	XP_011542154.1	malignant fibrous histiocytoma-amplified sequence 1 isoform X2
NM_004225.3	NP_004216.2	malignant fibrous histiocytoma-amplified sequence 1
XM_024447330.1	XP_024303098.1	malignant fibrous histiocytoma-amplified sequence 1 isoform X1

Pathways from BioSystems

Homologies

	MFHAS1	XP_001090936.1
Bos taurus (cattle)	MFHAS1	XP_003588007.1
Xenopus tropicalis (tropical clawed frog)	mfhas1	NP_001096407.1
Rattus norvegicus (Norway rat)	Mfhas1	NP_001100786.1
Gallus gallus (chicken)	LOC101750338	XP_004941212.1
Homo sapiens (human)	MFHAS1	NP_004216.2
Pan troglodytes (chimpanzee)	MFHAS1	XP_528066.2
Mus musculus (house mouse)	Mfhas1	NP_001074748.1
Danio rerio (zebrafish)	mfhas1	XP_001335202.2
Canis lupus familiaris (dog)	MFHAS1	XP_532819.3

Gene Ontology
Bibliography

Related articles in PubMed

High Glucose Stimulates Expression of MFHAS1 to Mitigate Inflammation via Akt/HO-1 Pathway in Human Umbilical Vein Endothelial Cells.
Wang HH, Sun PF, Chen WK, Zhong J, Shi QQ, Weng ML, Ma D, Miao CH
Inflammation(2017 Nov)

MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.
Shi Q, Xiong B, Zhong J, Wang H, Ma D, Miao C
Molecular immunology8879-88(2017 Aug)

Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1 macrophage polarization by activating JNK and p38 pathways.
Zhong J, Wang H, Chen W, Sun Z, Chen J, Xu Y, Weng M, Shi Q, Ma D, Miao C
Cell death & disease8(5)e2763(2017 May)

MFHAS1 Is Associated with Sepsis and Stimulates TLR2/NF-?B Signaling Pathway Following Negative Regulation.
Zhong J, Shi QQ, Zhu MM, Shen J, Wang HH, Ma D, Miao CH
PloS one10(11)e0143662(2015)

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Navarini AA, Simpson MA, Weale M, Knight J, Carlavan I, Reiniche P, Burden DA, Layton A, Bataille V, Allen M, Pleass R, Pink A, Creamer D, English J, Munn S, Walton S, , Willis C, D?ret S, Voegel JJ, Spector T, Smith CH, Trembath RC, Barker JN
Nature communications54020(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the results indicate that MFHAS1 suppresses TLR4 signaling pathway through induction of PP2A C subunit cytoplasm translocation and subsequent c-Jun degradation, leading finally to decrease AP-1 activity and cytokines expression.
Title: MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.

MASL1 expression is upregulated during primary human CD34+ cell erythroid differentiation and is an abundant protein in human red blood cells.
Title: MASL1 induces erythroid differentiation in human erythropoietin-dependent CD34+ cells through the Raf/MEK/ERK pathway.

aim of the review is to summarize the existing information on MASL1 and also to compile data that could be linked to MASL1 [review]
Title: MASL1: a neglected ROCO protein.

MFHAS1 regulates Toll-like receptor (TLR)-dependent signaling.
Title: Human leucine-rich repeat proteins: a genome-wide bioinformatic categorization and functional analysis in innate immunity.

Observational study of gene-disease association. (HuGE Navigator)
Title: A large-scale candidate gene association study of age at menarche and age at natural menopause.

The following MFHAS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MFHAS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19058	NM_004225.3 Latest version!	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$529.50-$741.30 $1059.00
OHu106074	XM_024447330.1 Latest version!	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu62668	XM_011543852.3 Latest version!	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
View Old Accession Versions >>
OHu62668	XM_011543852.2	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu19058	NM_004225.2	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$529.50-$741.30 $1059.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MFHAS1 ( NM_004225.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004216.2 Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), mRNA.

Price & Availability↑

CloneID	OHu19058
Clone ID Related Accession (Same CDS sequence)	NM_004225.2 , NM_004225.3
Accession Version	NM_004225.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-15
Organism	Homo sapiens(human)
Product	malignant fibrous histiocytoma-amplified sequence 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090567.7 and AK027007.1. This sequence is a reference standard in the RefSeqGene project. On Jul 28, 2006 this sequence version replaced NM_004225.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB016816.1, BC014226.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGGG 
GAGTTGCTGA GTCAGCCCAG ACCGGAAGGA GTGGCAGAGA TCATTTGCCC CAAGAACGGC 
AGCGAGCGAG TAAATGTTGC CTTGGTTTAC CCACCCACGC CGACTGTGAT CAGCCCCTGT 
TCCAAGAAGA ATGTTGGTGA AAAGCACAGA AACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004216.2
CDS	564..3722
Translation	MAGMDSGNLKTARLWRDAALRARKLRSNLRQLTLTAAGACPGAGADALESPASPQLVLPANLGDIEALNLGNNGLEEVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTALGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSLSCLSRLRTLDVDHNQLTAFPRQLLQLVALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPAGFCELASLESLMLDNNGLQALPAQFSCLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLISGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDHFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEERVEGCPGGGDKEKCYPPSPPPVSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRHFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKHDAEGLSRLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPRHLRRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDPSLLLHKLLLGTSGEGKAEGESSPPMARSTPSQELLRATQLHQYVEGFLLHGLLPAHVIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSTAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPLGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPARGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPGELLSQPRPEGVAEIICPKNGSERVNVALVYPPTPTVISPCSKKNVGEKHRNQ

Target ORF information:

RefSeq Version	NM_004225.2
Organism	Homo sapiens(human)
Definition	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004225.2

ORF Insert Sequence:

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGGG 
GAGTTGCTGA GTCAGCCCAG ACCGGAAGGA GTGGCAGAGA TCATTTGCCC CAAGAACGGC 
AGCGAGCGAG TAAATGTTGC CTTGGTTTAC CCACCCACGC CGACTGTGAT CAGCCCCTGT 
TCCAAGAAGA ATGTTGGTGA AAAGCACAGA AACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MFHAS1 ( NM_004225.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004216.2 Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), mRNA.

Price & Availability↑

CloneID	OHu19058
Clone ID Related Accession (Same CDS sequence)	NM_004225.2 , NM_004225.3
Accession Version	NM_004225.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	malignant fibrous histiocytoma-amplified sequence 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC090567.7 and AK027007.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004225.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB016816.1, BC014226.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000276282.7/ ENSP00000276282.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGGG 
GAGTTGCTGA GTCAGCCCAG ACCGGAAGGA GTGGCAGAGA TCATTTGCCC CAAGAACGGC 
AGCGAGCGAG TAAATGTTGC CTTGGTTTAC CCACCCACGC CGACTGTGAT CAGCCCCTGT 
TCCAAGAAGA ATGTTGGTGA AAAGCACAGA AACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004216.2
CDS	573..3731
Translation	MAGMDSGNLKTARLWRDAALRARKLRSNLRQLTLTAAGACPGAGADALESPASPQLVLPANLGDIEALNLGNNGLEEVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTALGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSLSCLSRLRTLDVDHNQLTAFPRQLLQLVALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPAGFCELASLESLMLDNNGLQALPAQFSCLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLISGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDHFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEERVEGCPGGGDKEKCYPPSPPPVSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRHFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKHDAEGLSRLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPRHLRRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDPSLLLHKLLLGTSGEGKAEGESSPPMARSTPSQELLRATQLHQYVEGFLLHGLLPAHVIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSTAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPLGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPARGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPGELLSQPRPEGVAEIICPKNGSERVNVALVYPPTPTVISPCSKKNVGEKHRNQ

Target ORF information:

RefSeq Version	NM_004225.3
Organism	Homo sapiens(human)
Definition	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004225.3

ORF Insert Sequence:

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGGG 
GAGTTGCTGA GTCAGCCCAG ACCGGAAGGA GTGGCAGAGA TCATTTGCCC CAAGAACGGC 
AGCGAGCGAG TAAATGTTGC CTTGGTTTAC CCACCCACGC CGACTGTGAT CAGCCCCTGT 
TCCAAGAAGA ATGTTGGTGA AAAGCACAGA AACCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MFHAS1 ( XM_024447330.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024303098.1 Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu106074
Clone ID Related Accession (Same CDS sequence)	XM_024447330.1
Accession Version	XM_024447330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	malignant fibrous histiocytoma-amplified sequence 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGGG 
GAGTTGCTGA GTCAGCCCAG ACCGGAAGGA GTGGCAGAGA TCATTTGCCC CAAGAACGGC 
AGCGAGCGAG TAAATGTTGC CTTGGTTTAC CCACCCACGC CGACTGTGAT CAGCCCCTGT 
TCCAAGTATC TTCATACTTT CTTGGAAAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024303098.1
CDS	118..3270
Translation	MAGMDSGNLKTARLWRDAALRARKLRSNLRQLTLTAAGACPGAGADALESPASPQLVLPANLGDIEALNLGNNGLEEVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTALGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSLSCLSRLRTLDVDHNQLTAFPRQLLQLVALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPAGFCELASLESLMLDNNGLQALPAQFSCLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLISGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDHFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEERVEGCPGGGDKEKCYPPSPPPVSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRHFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKHDAEGLSRLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPRHLRRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDPSLLLHKLLLGTSGEGKAEGESSPPMARSTPSQELLRATQLHQYVEGFLLHGLLPAHVIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSTAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPLGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPARGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPGELLSQPRPEGVAEIICPKNGSERVNVALVYPPTPTVISPCSKYLHTFLEN

Target ORF information:

RefSeq Version	XM_024447330.1
Organism	Homo sapiens(human)
Definition	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024447330.1

ORF Insert Sequence:

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGGG 
GAGTTGCTGA GTCAGCCCAG ACCGGAAGGA GTGGCAGAGA TCATTTGCCC CAAGAACGGC 
AGCGAGCGAG TAAATGTTGC CTTGGTTTAC CCACCCACGC CGACTGTGAT CAGCCCCTGT 
TCCAAGTATC TTCATACTTT CTTGGAAAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MFHAS1 ( XM_011543852.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542154.1 Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu62668
Clone ID Related Accession (Same CDS sequence)	XM_011543852.2 , XM_011543852.3
Accession Version	XM_011543852.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	malignant fibrous histiocytoma-amplified sequence 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGAT 
TCCTCAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542154.1
CDS	538..3549
Translation	MAGMDSGNLKTARLWRDAALRARKLRSNLRQLTLTAAGACPGAGADALESPASPQLVLPANLGDIEALNLGNNGLEEVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTALGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSLSCLSRLRTLDVDHNQLTAFPRQLLQLVALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPAGFCELASLESLMLDNNGLQALPAQFSCLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLISGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDHFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEERVEGCPGGGDKEKCYPPSPPPVSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRHFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKHDAEGLSRLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPRHLRRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDPSLLLHKLLLGTSGEGKAEGESSPPMARSTPSQELLRATQLHQYVEGFLLHGLLPAHVIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSTAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPLGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPARGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPDSSR

Target ORF information:

RefSeq Version	XM_011543852.2
Organism	Homo sapiens(human)
Definition	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543852.2

ORF Insert Sequence:

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGAT 
TCCTCAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MFHAS1 ( XM_011543852.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011542154.1 Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X2, mRNA.

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CloneID	OHu62668
Clone ID Related Accession (Same CDS sequence)	XM_011543852.2 , XM_011543852.3
Accession Version	XM_011543852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	malignant fibrous histiocytoma-amplified sequence 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGAT 
TCCTCAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011542154.1
CDS	118..3129
Translation	MAGMDSGNLKTARLWRDAALRARKLRSNLRQLTLTAAGACPGAGADALESPASPQLVLPANLGDIEALNLGNNGLEEVPEGLGSALGSLRVLVLRRNRFARLPPAVAELGHHLTELDVSHNRLTALGAEVVSALRELRKLNLSHNQLPALPAQLGALAHLEELDVSFNRLAHLPDSLSCLSRLRTLDVDHNQLTAFPRQLLQLVALEELDVSSNRLRGLPEDISALRALKILWLSGAELGTLPAGFCELASLESLMLDNNGLQALPAQFSCLQRLKMLNLSSNLFEEFPAALLPLAGLEELYLSRNQLTSVPSLISGLGRLLTLWLDNNRIRYLPDSIVELTGLEELVLQGNQIAVLPDHFGQLSRVGLWKIKDNPLIQPPYEVCMKGIPYIAAYQKELAHSQPAVQPRLKLLLMGHKAAGKTLLRHCLTEERVEGCPGGGDKEKCYPPSPPPVSKGIEVTSWTADASRGLRFIVYDLAGDESYEVIQPFFLSPGALYVLVVNLATYEPRHFPTTVGSFLHRVGARVPHAVVCIVGTHADLCGERELEEKCLDIHRQIALQEKHDAEGLSRLAKVVDEALARDFELRSASPHAAYYGVSDKNLRRRKAHFQYLLNHRLQILSPVLPVSCRDPRHLRRLRDKLLSVAEHREIFPNLHRVLPRSWQVLEELHFQPPQAQRLWLSWWDSARLGLQAGLTEDRLQSALSYLHESGKLLYFEDSPALKEHVFHNLTRLIDILNVFFQRDPSLLLHKLLLGTSGEGKAEGESSPPMARSTPSQELLRATQLHQYVEGFLLHGLLPAHVIRLLLKPHVQAQQDLQLLLELLEKMGLCYCLNKPKGKPLNGSTAWYKFPCYVQNEVPHAEAWINGTNLAGQSFVAEQLQIEYSFPFTFPLGLFARYSVQINSHVVHRSDGKFQIFAYRGKVPVVVSYRPARGVLQPDTLSIASHASLPNIWTAWQAITPLVEELNVLLQEWPGLHYTVHILCSKCLKRGSPNPHAFPDSSR

Target ORF information:

RefSeq Version	XM_011543852.3
Organism	Homo sapiens(human)
Definition	Homo sapiens malignant fibrous histiocytoma amplified sequence 1 (MFHAS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543852.3

ORF Insert Sequence:

ATGGCTGGGA TGGACAGTGG CAACCTGAAG ACCGCGAGGC TGTGGCGGGA CGCCGCCCTG 
CGTGCCAGGA AGCTGCGGAG CAACCTGCGC CAGCTCACGC TTACCGCCGC CGGGGCCTGC 
CCCGGGGCCG GGGCCGACGC GCTCGAGTCC CCCGCCTCCC CCCAGCTCGT GCTGCCGGCC 
AACCTCGGGG ACATTGAGGC ACTGAACCTG GGGAACAACG GCCTGGAGGA GGTACCCGAG 
GGGCTGGGGT CGGCGCTGGG CAGCCTGCGC GTCCTGGTCC TGCGCAGGAA CCGCTTCGCC 
CGGCTGCCCC CGGCGGTGGC CGAGCTCGGC CACCACCTCA CCGAGCTGGA CGTGAGCCAC 
AACCGGCTGA CCGCCCTGGG CGCGGAGGTG GTGAGTGCTC TGAGGGAGCT GCGGAAGCTC 
AACCTCAGCC ACAACCAGCT GCCCGCCCTG CCCGCCCAGC TGGGCGCTCT CGCTCACCTG 
GAGGAGCTGG ATGTCAGCTT TAACCGGCTG GCGCACCTGC CTGACTCCCT CTCCTGCCTC 
TCCCGCCTGC GCACCCTGGA CGTGGATCAC AACCAGCTCA CTGCCTTCCC CCGGCAGCTG 
CTGCAGCTGG TGGCCCTGGA GGAGCTGGAC GTGTCCAGCA ACCGGCTGCG GGGCCTGCCT 
GAGGATATCA GTGCCCTGCG TGCCCTCAAG ATCCTCTGGC TGAGTGGGGC CGAGCTTGGC 
ACGCTGCCCG CCGGCTTCTG CGAGCTGGCC AGTTTGGAGA GCCTCATGCT AGACAACAAC 
GGGCTGCAGG CTCTGCCCGC CCAGTTCAGC TGCCTGCAGC GGCTCAAAAT GCTCAACCTC 
TCCTCCAACC TCTTCGAGGA GTTCCCTGCC GCGCTGCTGC CCCTGGCTGG TCTGGAGGAG 
CTCTACCTTA GTCGCAACCA GCTCACCTCG GTGCCATCCC TTATCTCGGG CCTGGGCCGG 
CTTCTCACCT TGTGGCTGGA TAATAACCGC ATCCGCTACC TGCCGGACTC CATCGTGGAG 
CTGACCGGCC TGGAGGAGCT CGTGCTGCAG GGGAACCAGA TCGCGGTGCT GCCCGACCAC 
TTTGGCCAGC TCTCCCGGGT GGGTTTGTGG AAGATCAAAG ACAACCCACT GATCCAGCCC 
CCCTACGAGG TCTGCATGAA GGGGATCCCC TACATCGCAG CCTACCAGAA GGAACTGGCT 
CATTCCCAGC CGGCGGTGCA GCCCCGGCTC AAGCTGCTCC TGATGGGGCA TAAGGCTGCA 
GGAAAGACTT TGCTGCGCCA CTGCCTCACC GAGGAGAGAG TGGAGGGATG CCCAGGAGGA 
GGGGACAAGG AGAAGTGCTA CCCACCGTCA CCTCCCCCTG TGAGCAAGGG CATCGAGGTG 
ACCAGCTGGA CGGCCGATGC CTCCCGGGGC CTGCGGTTCA TCGTGTATGA CTTAGCTGGG 
GATGAAAGTT ATGAGGTGAT CCAGCCCTTC TTCCTGTCCC CAGGGGCCCT ATACGTGCTG 
GTGGTCAACT TGGCCACCTA TGAGCCTCGC CACTTTCCTA CCACCGTGGG CTCCTTCTTG 
CATCGGGTCG GGGCGAGAGT GCCCCACGCG GTGGTGTGCA TCGTGGGCAC CCACGCAGAC 
CTGTGCGGAG AGCGTGAGCT GGAGGAGAAA TGTCTGGACA TTCACCGCCA GATCGCCCTG 
CAGGAGAAGC ACGACGCGGA GGGACTGAGC CGCTTGGCCA AGGTGGTGGA CGAGGCACTG 
GCCCGGGACT TCGAGCTGCG CTCTGCCAGC CCCCACGCAG CCTACTATGG CGTTTCGGAC 
AAGAACCTTC GACGGCGCAA GGCCCATTTT CAATACCTGC TCAACCACCG GCTGCAGATC 
CTCTCCCCCG TGTTGCCTGT TAGCTGCAGG GACCCGCGCC ACTTACGACG CCTTCGGGAC 
AAGTTGCTGT CAGTTGCTGA GCACCGAGAG ATCTTCCCCA ACTTACACAG AGTACTGCCT 
CGATCCTGGC AGGTGCTGGA GGAACTGCAT TTCCAGCCAC CTCAGGCCCA GCGACTGTGG 
CTAAGCTGGT GGGACTCGGC GCGCTTGGGC CTGCAGGCGG GTCTGACCGA GGACCGACTG 
CAGAGTGCCC TCTCCTACCT GCATGAGAGC GGCAAGCTAC TCTACTTTGA GGACAGTCCG 
GCTCTCAAGG AGCACGTCTT CCACAACCTC ACCCGCCTCA TCGACATCCT CAATGTCTTC 
TTCCAGAGGG ATCCCTCTTT GCTGCTGCAT AAGCTGCTCC TAGGGACCAG TGGAGAGGGC 
AAGGCGGAGG GGGAAAGCTC CCCGCCCATG GCGCGGTCCA CCCCCAGCCA GGAACTGCTC 
CGGGCCACCC AGCTCCATCA GTATGTGGAG GGCTTTCTGT TGCATGGGCT CTTGCCAGCT 
CATGTCATTC GGTTGCTGCT TAAGCCTCAT GTCCAGGCCC AGCAGGACTT GCAGCTGTTG 
CTGGAGCTGC TGGAGAAGAT GGGACTCTGT TACTGCCTCA ATAAACCCAA GGGCAAGCCT 
TTGAATGGGT CCACAGCTTG GTACAAGTTC CCATGCTATG TGCAGAACGA GGTGCCCCAT 
GCAGAAGCCT GGATTAATGG GACCAACCTA GCTGGGCAGT CTTTTGTGGC TGAGCAGTTG 
CAGATTGAAT ATAGCTTTCC TTTTACTTTT CCACTTGGGT TGTTTGCACG CTACAGTGTC 
CAGATCAACA GCCATGTGGT GCACAGGTCG GATGGTAAAT TTCAGATCTT TGCCTATAGA 
GGGAAAGTTC CTGTGGTTGT GAGTTACAGA CCTGCCAGGG GAGTCCTGCA GCCAGACACC 
CTGTCCATTG CTAGCCATGC ATCATTACCA AATATATGGA CCGCATGGCA AGCCATAACC 
CCCTTGGTGG AGGAACTGAA TGTCCTACTT CAGGAATGGC CTGGACTGCA CTACACCGTG 
CACATTCTCT GTTCTAAGTG CCTTAAGAGA GGATCGCCCA ATCCACATGC TTTTCCAGAT 
TCCTCAAGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High Glucose Stimulates Expression of MFHAS1 to Mitigate Inflammation via Akt/HO-1 Pathway in Human Umbilical Vein Endothelial Cells.
Inflammation(2017 Nov)
Wang HH,Sun PF,Chen WK,Zhong J,Shi QQ,Weng ML,Ma D,Miao CH

MFHAS1 suppresses TLR4 signaling pathway via induction of PP2A C subunit cytoplasm translocation and inhibition of c-Jun dephosphorylation at Thr239.
Molecular immunology8879-88(2017 Aug)
Shi Q,Xiong B,Zhong J,Wang H,Ma D,Miao C

Ubiquitylation of MFHAS1 by the ubiquitin ligase praja2 promotes M1 macrophage polarization by activating JNK and p38 pathways.
Cell death & disease8(5)e2763(2017 May)
Zhong J,Wang H,Chen W,Sun Z,Chen J,Xu Y,Weng M,Shi Q,Ma D,Miao C

MFHAS1 Is Associated with Sepsis and Stimulates TLR2/NF-?B Signaling Pathway Following Negative Regulation.
PloS one10(11)e0143662(2015)
Zhong J,Shi QQ,Zhu MM,Shen J,Wang HH,Ma D,Miao CH

Genome-wide association study identifies three novel susceptibility loci for severe Acne vulgaris.
Nature communications54020(2014 Jun)
Navarini AA,Simpson MA,Weale M,Knight J,Carlavan I,Reiniche P,Burden DA,Layton A,Bataille V,Allen M,Pleass R,Pink A,Creamer D,English J,Munn S,Walton S,,Willis C,D?ret S,Voegel JJ,Spector T,Smith CH,Trembath RC,Barker JN

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MFHAS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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