CRLF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CRLF1
Entrez Gene ID	9244
Full Name	cytokine receptor like factor 1
Synonyms	CISS,CISS1,CLF,CLF-1,NR6,zcytor5
General protein information	Preferred Names cytokine receptor like factor 1 Names cytokine receptor-like factor 1 class I cytokine receptor cytokine type 1 receptor CRLP-1 cytokine-like factor 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.11
Summary	This gene encodes a member of the cytokine type I receptor family. The protein forms a secreted complex with cardiotrophin-like cytokine factor 1 and acts on cells expressing ciliary neurotrophic factor receptors. The complex can promote survival of neuronal cells. Mutations in this gene result in Crisponi syndrome and cold-induced sweating syndrome. [provided by RefSeq, Oct 2009].
Disorder MIM:	604237
Disorder Html:	Cold-induced sweating syndrome 1, 272430 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011528424.2	XP_011526726.1	cytokine receptor-like factor 1 isoform X3
NM_004750.5	NP_004741.1	cytokine receptor-like factor 1 precursor
XM_011528424.3	XP_011526726.1	cytokine receptor-like factor 1 isoform X3
XM_011528423.2	XP_011526725.1	cytokine receptor-like factor 1 isoform X2
XM_011528422.2	XP_011526724.1	cytokine receptor-like factor 1 isoform X1
NM_004750.4	NP_004741.1	cytokine receptor-like factor 1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Crlf1	NP_001099544.1
Gallus gallus (chicken)	CRLF1	XP_004948964.1
Canis lupus familiaris (dog)	CRLF1	XP_005633255.1
Bos taurus (cattle)	CRLF1	XP_002688582.2
Pan troglodytes (chimpanzee)	CRLF1	XP_001135263.3
Mus musculus (house mouse)	Crlf1	NP_061297.1
Xenopus tropicalis (tropical clawed frog)	crlf1	XP_002940253.2
Homo sapiens (human)	CRLF1	NP_004741.1
	CRLF1	XP_001115478.2
Danio rerio (zebrafish)	crlf1a	NP_001002650.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutations in CRLF1 cause familial achalasia.
Busch A, ?arkovi? M, Lowe C, Jankofsky M, Ganschow R, Buers I, Kurth I, Reutter H, Rutsch F, H?bner CA
Clinical genetics92(1)104-108(2017 Jul)

Crisponi/CISS1 syndrome: A case series.
Alhashem AM, Majeed-Saidan MA, Ammari AN, Alrakaf MS, Nojoom M, Maddirevula S, Faqeih E, Alkuraya FS, Garne E, Kurdi AM
American journal of medical genetics. Part A170A(5)1236-41(2016 May)

Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor ? Signaling and sorLA-Mediated Turnover.
Larsen JV, Kristensen AM, Pallesen LT, Bauer J, V?gter CB, Nielsen MS, Madsen P, Petersen CM
Molecular and cellular biology36(8)1272-86(2016 Apr)

Expanding the mutational spectrum of CRLF1 in Crisponi/CISS1 syndrome.
Piras R, Chiappe F, Torraca IL, Buers I, Usala G, Angius A, Akin MA, Basel-Vanagaite L, Benedicenti F, Chiodin E, El Assy O, Feingold-Zadok M, Guibert J, Kamien B, Kasapkara CS, Kili? E, Boduro?lu K, Kurtoglu S, Manzur AY, Onal EE, Paderi E, Roche CH, T?mer L, Unal S, Utine GE, Zanda G, Zankl A, Zampino G, Crisponi G, Crisponi L, Rutsch F
Human mutation35(4)424-33(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show in this series of 12 patients from four families, all previously unpublished, that the homogeneity of the recently described c.983dupG (p.Ser328Argfs *2) mutation in CRLF1 was associated with a highly variable degree of severity, and that the phenotype significantly overlaps with the recently described COG6-related anhidrosis syndrome (MIM#615328
Title: Crisponi/CISS1 syndrome: A case series.

CLF-1, based on its binding site for CLC and on two additional and independent sites for CNTFRalpha and sorLA, is a key player in CLC and CNTFRalpha signaling and turnover.
Title: Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor ? Signaling and sorLA-Mediated Turnover.

Article reports 11 novel mutations in CRLF1 expanding the mutational spectrum of CRLF1 in the Crisponi/cold-induced sweating type 1 syndrome to a total of 35 variants.
Title: Expanding the mutational spectrum of CRLF1 in Crisponi/CISS1 syndrome.

The mutation detected in CRLF1 has not been described in patients with CISS1, but in one with CS. These data seem to support the theory that CS and CISS1 are variants of the same disorder.
Title: Cold-induced sweating syndrome type 1, with a CRLF1 level mutation, previously associated with Crisponi syndrome.

Data indicate that CRLF1 serves its protective role by a cell autonomous mechanism that is independent of the gp130/JAK signaling pathway.
Title: Cytokine receptor-like factor 1 (CRLF1) protects against 6-hydroxydopamine toxicity independent of the gp130/JAK signaling pathway.

The following CRLF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CRLF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18874	NM_004750.5 Latest version!	Homo sapiens cytokine receptor like factor 1 (CRLF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu62657	XM_011528422.2 Latest version!	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu62658	XM_011528423.2 Latest version!	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu62659	XM_011528424.3 Latest version!	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu62659	XM_011528424.2	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu18874	NM_004750.4	Homo sapiens cytokine receptor like factor 1 (CRLF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CRLF1 ( NM_004750.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004741.1 Homo sapiens cytokine receptor like factor 1 (CRLF1), mRNA.

Price & Availability↑

CloneID	OHu18874
Clone ID Related Accession (Same CDS sequence)	NM_004750.5 , NM_004750.4
Accession Version	NM_004750.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	cytokine receptor-like factor 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF073515.1 and AY358291.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_004750.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF073515.1, AY358291.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000392386.8/ ENSP00000376188.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCGCCG GCCGCCGGGG CCCCGCCGCC CAATCCGCGC GGCGGCCGCC GCCGTTGCTG 
CCCCTGCTGC TGCTGCTCTG CGTCCTCGGG GCGCCGCGAG CCGGATCAGG AGCCCACACA 
GCTGTGATCA GTCCCCAGGA TCCCACGCTT CTCATCGGCT CCTCCCTGCT GGCCACCTGC 
TCAGTGCACG GAGACCCACC AGGAGCCACC GCCGAGGGCC TCTACTGGAC CCTCAACGGG 
CGCCGCCTGC CCCCTGAGCT CTCCCGTGTA CTCAACGCCT CCACCTTGGC TCTGGCCCTG 
GCCAACCTCA ATGGGTCCAG GCAGCGGTCG GGGGACAACC TCGTGTGCCA CGCCCGTGAC 
GGCAGCATCC TGGCTGGCTC CTGCCTCTAT GTTGGCCTGC CCCCAGAGAA ACCCGTCAAC 
ATCAGCTGCT GGTCCAAGAA CATGAAGGAC TTGACCTGCC GCTGGACGCC AGGGGCCCAC 
GGGGAGACCT TCCTCCACAC CAACTACTCC CTCAAGTACA AGCTTAGGTG GTATGGCCAG 
GACAACACAT GTGAGGAGTA CCACACAGTG GGGCCCCACT CCTGCCACAT CCCCAAGGAC 
CTGGCTCTCT TTACGCCCTA TGAGATCTGG GTGGAGGCCA CCAACCGCCT GGGCTCTGCC 
CGCTCCGATG TACTCACGCT GGATATCCTG GATGTGGTGA CCACGGACCC CCCGCCCGAC 
GTGCACGTGA GCCGCGTCGG GGGCCTGGAG GACCAGCTGA GCGTGCGCTG GGTGTCGCCA 
CCCGCCCTCA AGGATTTCCT CTTTCAAGCC AAATACCAGA TCCGCTACCG AGTGGAGGAC 
AGTGTGGACT GGAAGGTGGT GGACGATGTG AGCAACCAGA CCTCCTGCCG CCTGGCCGGC 
CTGAAACCCG GCACCGTGTA CTTCGTGCAA GTGCGCTGCA ACCCCTTTGG CATCTATGGC 
TCCAAGAAAG CCGGGATCTG GAGTGAGTGG AGCCACCCCA CAGCCGCCTC CACTCCCCGC 
AGTGAGCGCC CGGGCCCGGG CGGCGGGGCG TGCGAACCGC GGGGCGGAGA GCCGAGCTCG 
GGGCCGGTGC GGCGCGAGCT CAAGCAGTTC CTGGGCTGGC TCAAGAAGCA CGCGTACTGC 
TCCAACCTCA GCTTCCGCCT CTACGACCAG TGGCGAGCCT GGATGCAGAA GTCGCACAAG 
ACCCGCAACC AGGACGAGGG GATCCTGCCC TCGGGCAGAC GGGGCACGGC GAGAGGTCCT 
GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004741.1
CDS	144..1412
Translation	MPAGRRGPAAQSARRPPPLLPLLLLLCVLGAPRAGSGAHTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQDEGILPSGRRGTARGPAR

Target ORF information:

RefSeq Version	NM_004750.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cytokine receptor like factor 1 (CRLF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004750.5

ORF Insert Sequence:

ATGCCCGCCG GCCGCCGGGG CCCCGCCGCC CAATCCGCGC GGCGGCCGCC GCCGTTGCTG 
CCCCTGCTGC TGCTGCTCTG CGTCCTCGGG GCGCCGCGAG CCGGATCAGG AGCCCACACA 
GCTGTGATCA GTCCCCAGGA TCCCACGCTT CTCATCGGCT CCTCCCTGCT GGCCACCTGC 
TCAGTGCACG GAGACCCACC AGGAGCCACC GCCGAGGGCC TCTACTGGAC CCTCAACGGG 
CGCCGCCTGC CCCCTGAGCT CTCCCGTGTA CTCAACGCCT CCACCTTGGC TCTGGCCCTG 
GCCAACCTCA ATGGGTCCAG GCAGCGGTCG GGGGACAACC TCGTGTGCCA CGCCCGTGAC 
GGCAGCATCC TGGCTGGCTC CTGCCTCTAT GTTGGCCTGC CCCCAGAGAA ACCCGTCAAC 
ATCAGCTGCT GGTCCAAGAA CATGAAGGAC TTGACCTGCC GCTGGACGCC AGGGGCCCAC 
GGGGAGACCT TCCTCCACAC CAACTACTCC CTCAAGTACA AGCTTAGGTG GTATGGCCAG 
GACAACACAT GTGAGGAGTA CCACACAGTG GGGCCCCACT CCTGCCACAT CCCCAAGGAC 
CTGGCTCTCT TTACGCCCTA TGAGATCTGG GTGGAGGCCA CCAACCGCCT GGGCTCTGCC 
CGCTCCGATG TACTCACGCT GGATATCCTG GATGTGGTGA CCACGGACCC CCCGCCCGAC 
GTGCACGTGA GCCGCGTCGG GGGCCTGGAG GACCAGCTGA GCGTGCGCTG GGTGTCGCCA 
CCCGCCCTCA AGGATTTCCT CTTTCAAGCC AAATACCAGA TCCGCTACCG AGTGGAGGAC 
AGTGTGGACT GGAAGGTGGT GGACGATGTG AGCAACCAGA CCTCCTGCCG CCTGGCCGGC 
CTGAAACCCG GCACCGTGTA CTTCGTGCAA GTGCGCTGCA ACCCCTTTGG CATCTATGGC 
TCCAAGAAAG CCGGGATCTG GAGTGAGTGG AGCCACCCCA CAGCCGCCTC CACTCCCCGC 
AGTGAGCGCC CGGGCCCGGG CGGCGGGGCG TGCGAACCGC GGGGCGGAGA GCCGAGCTCG 
GGGCCGGTGC GGCGCGAGCT CAAGCAGTTC CTGGGCTGGC TCAAGAAGCA CGCGTACTGC 
TCCAACCTCA GCTTCCGCCT CTACGACCAG TGGCGAGCCT GGATGCAGAA GTCGCACAAG 
ACCCGCAACC AGGACGAGGG GATCCTGCCC TCGGGCAGAC GGGGCACGGC GAGAGGTCCT 
GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRLF1 ( NM_004750.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004741.1 Homo sapiens cytokine receptor like factor 1 (CRLF1), mRNA.

Price & Availability↑

CloneID	OHu18874
Clone ID Related Accession (Same CDS sequence)	NM_004750.5 , NM_004750.4
Accession Version	NM_004750.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	cytokine receptor-like factor 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF073515.1 and AY358291.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2009 this sequence version replaced NM_004750.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF073515.1, BC044634.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCGCCG GCCGCCGGGG CCCCGCCGCC CAATCCGCGC GGCGGCCGCC GCCGTTGCTG 
CCCCTGCTGC TGCTGCTCTG CGTCCTCGGG GCGCCGCGAG CCGGATCAGG AGCCCACACA 
GCTGTGATCA GTCCCCAGGA TCCCACGCTT CTCATCGGCT CCTCCCTGCT GGCCACCTGC 
TCAGTGCACG GAGACCCACC AGGAGCCACC GCCGAGGGCC TCTACTGGAC CCTCAACGGG 
CGCCGCCTGC CCCCTGAGCT CTCCCGTGTA CTCAACGCCT CCACCTTGGC TCTGGCCCTG 
GCCAACCTCA ATGGGTCCAG GCAGCGGTCG GGGGACAACC TCGTGTGCCA CGCCCGTGAC 
GGCAGCATCC TGGCTGGCTC CTGCCTCTAT GTTGGCCTGC CCCCAGAGAA ACCCGTCAAC 
ATCAGCTGCT GGTCCAAGAA CATGAAGGAC TTGACCTGCC GCTGGACGCC AGGGGCCCAC 
GGGGAGACCT TCCTCCACAC CAACTACTCC CTCAAGTACA AGCTTAGGTG GTATGGCCAG 
GACAACACAT GTGAGGAGTA CCACACAGTG GGGCCCCACT CCTGCCACAT CCCCAAGGAC 
CTGGCTCTCT TTACGCCCTA TGAGATCTGG GTGGAGGCCA CCAACCGCCT GGGCTCTGCC 
CGCTCCGATG TACTCACGCT GGATATCCTG GATGTGGTGA CCACGGACCC CCCGCCCGAC 
GTGCACGTGA GCCGCGTCGG GGGCCTGGAG GACCAGCTGA GCGTGCGCTG GGTGTCGCCA 
CCCGCCCTCA AGGATTTCCT CTTTCAAGCC AAATACCAGA TCCGCTACCG AGTGGAGGAC 
AGTGTGGACT GGAAGGTGGT GGACGATGTG AGCAACCAGA CCTCCTGCCG CCTGGCCGGC 
CTGAAACCCG GCACCGTGTA CTTCGTGCAA GTGCGCTGCA ACCCCTTTGG CATCTATGGC 
TCCAAGAAAG CCGGGATCTG GAGTGAGTGG AGCCACCCCA CAGCCGCCTC CACTCCCCGC 
AGTGAGCGCC CGGGCCCGGG CGGCGGGGCG TGCGAACCGC GGGGCGGAGA GCCGAGCTCG 
GGGCCGGTGC GGCGCGAGCT CAAGCAGTTC CTGGGCTGGC TCAAGAAGCA CGCGTACTGC 
TCCAACCTCA GCTTCCGCCT CTACGACCAG TGGCGAGCCT GGATGCAGAA GTCGCACAAG 
ACCCGCAACC AGGACGAGGG GATCCTGCCC TCGGGCAGAC GGGGCACGGC GAGAGGTCCT 
GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004741.1
CDS	195..1463
Translation	MPAGRRGPAAQSARRPPPLLPLLLLLCVLGAPRAGSGAHTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQDEGILPSGRRGTARGPAR

Target ORF information:

RefSeq Version	NM_004750.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytokine receptor like factor 1 (CRLF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004750.4

ORF Insert Sequence:

ATGCCCGCCG GCCGCCGGGG CCCCGCCGCC CAATCCGCGC GGCGGCCGCC GCCGTTGCTG 
CCCCTGCTGC TGCTGCTCTG CGTCCTCGGG GCGCCGCGAG CCGGATCAGG AGCCCACACA 
GCTGTGATCA GTCCCCAGGA TCCCACGCTT CTCATCGGCT CCTCCCTGCT GGCCACCTGC 
TCAGTGCACG GAGACCCACC AGGAGCCACC GCCGAGGGCC TCTACTGGAC CCTCAACGGG 
CGCCGCCTGC CCCCTGAGCT CTCCCGTGTA CTCAACGCCT CCACCTTGGC TCTGGCCCTG 
GCCAACCTCA ATGGGTCCAG GCAGCGGTCG GGGGACAACC TCGTGTGCCA CGCCCGTGAC 
GGCAGCATCC TGGCTGGCTC CTGCCTCTAT GTTGGCCTGC CCCCAGAGAA ACCCGTCAAC 
ATCAGCTGCT GGTCCAAGAA CATGAAGGAC TTGACCTGCC GCTGGACGCC AGGGGCCCAC 
GGGGAGACCT TCCTCCACAC CAACTACTCC CTCAAGTACA AGCTTAGGTG GTATGGCCAG 
GACAACACAT GTGAGGAGTA CCACACAGTG GGGCCCCACT CCTGCCACAT CCCCAAGGAC 
CTGGCTCTCT TTACGCCCTA TGAGATCTGG GTGGAGGCCA CCAACCGCCT GGGCTCTGCC 
CGCTCCGATG TACTCACGCT GGATATCCTG GATGTGGTGA CCACGGACCC CCCGCCCGAC 
GTGCACGTGA GCCGCGTCGG GGGCCTGGAG GACCAGCTGA GCGTGCGCTG GGTGTCGCCA 
CCCGCCCTCA AGGATTTCCT CTTTCAAGCC AAATACCAGA TCCGCTACCG AGTGGAGGAC 
AGTGTGGACT GGAAGGTGGT GGACGATGTG AGCAACCAGA CCTCCTGCCG CCTGGCCGGC 
CTGAAACCCG GCACCGTGTA CTTCGTGCAA GTGCGCTGCA ACCCCTTTGG CATCTATGGC 
TCCAAGAAAG CCGGGATCTG GAGTGAGTGG AGCCACCCCA CAGCCGCCTC CACTCCCCGC 
AGTGAGCGCC CGGGCCCGGG CGGCGGGGCG TGCGAACCGC GGGGCGGAGA GCCGAGCTCG 
GGGCCGGTGC GGCGCGAGCT CAAGCAGTTC CTGGGCTGGC TCAAGAAGCA CGCGTACTGC 
TCCAACCTCA GCTTCCGCCT CTACGACCAG TGGCGAGCCT GGATGCAGAA GTCGCACAAG 
ACCCGCAACC AGGACGAGGG GATCCTGCCC TCGGGCAGAC GGGGCACGGC GAGAGGTCCT 
GCCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRLF1 ( XM_011528422.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526724.1 Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu62657
Clone ID Related Accession (Same CDS sequence)	XM_011528422.2
Accession Version	XM_011528422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	cytokine receptor-like factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGGACGTGCA GTTTGCCAGG GCTGCCTTTA GTTCCAGAGA CACAGCTGTG 
ATCAGTCCCC AGGATCCCAC GCTTCTCATC GGCTCCTCCC TGCTGGCCAC CTGCTCAGTG 
CACGGAGACC CACCAGGAGC CACCGCCGAG GGCCTCTACT GGACCCTCAA CGGGCGCCGC 
CTGCCCCCTG AGCTCTCCCG TGTACTCAAC GCCTCCACCT TGGCTCTGGC CCTGGCCAAC 
CTCAATGGGT CCAGGCAGCG GTCGGGGGAC AACCTCGTGT GCCACGCCCG TGACGGCAGC 
ATCCTGGCTG GCTCCTGCCT CTATGTTGGC CTGCCCCCAG AGAAACCCGT CAACATCAGC 
TGCTGGTCCA AGAACATGAA GGACTTGACC TGCCGCTGGA CGCCAGGGGC CCACGGGGAG 
ACCTTCCTCC ACACCAACTA CTCCCTCAAG TACAAGCTTA GGTGGTATGG CCAGGACAAC 
ACATGTGAGG AGTACCACAC AGTGGGGCCC CACTCCTGCC ACATCCCCAA GGACCTGGCT 
CTCTTTACGC CCTATGAGAT CTGGGTGGAG GCCACCAACC GCCTGGGCTC TGCCCGCTCC 
GATGTACTCA CGCTGGATAT CCTGGATGTG GTGACCACGG ACCCCCCGCC CGACGTGCAC 
GTGAGCCGCG TCGGGGGCCT GGAGGACCAG CTGAGCGTGC GCTGGGTGTC GCCACCCGCC 
CTCAAGGATT TCCTCTTTCA AGCCAAATAC CAGATCCGCT ACCGAGTGGA GGACAGTGTG 
GACTGGAAGG TGGTGGACGA TGTGAGCAAC CAGACCTCCT GCCGCCTGGC CGGCCTGAAA 
CCCGGCACCG TGTACTTCGT GCAAGTGCGC TGCAACCCCT TTGGCATCTA TGGCTCCAAG 
AAAGCCGGGA TCTGGAGTGA GTGGAGCCAC CCCACAGCCG CCTCCACTCC CCGCAGTGAG 
CGCCCGGGCC CGGGCGGCGG GGCGTGCGAA CCGCGGGGCG GAGAGCCGAG CTCGGGGCCG 
GTGCGGCGCG AGCTCAAGCA GTTCCTGGGC TGGCTCAAGA AGCACGCGTA CTGCTCCAAC 
CTCAGCTTCC GCCTCTACGA CCAGTGGCGA GCCTGGATGC AGAAGTCGCA CAAGACCCGC 
AACCAGCACA GGACGAGGGG ATCCTGCCCT CGGGCAGACG GGGCACGGCG AGAGCCTAAG 
AACATTCAAT GCCCTGAAAA TGTCCGAAGT GGAATCTCCT CCGAAATGAA TGAACATACT 
CACCCTAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526724.1
CDS	343..1614
Translation	MGVGDVQFARAAFSSRDTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQHRTRGSCPRADGARREPKNIQCPENVRSGISSEMNEHTHPS

Target ORF information:

RefSeq Version	XM_011528422.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528422.2

ORF Insert Sequence:

ATGGGAGTTG GGGACGTGCA GTTTGCCAGG GCTGCCTTTA GTTCCAGAGA CACAGCTGTG 
ATCAGTCCCC AGGATCCCAC GCTTCTCATC GGCTCCTCCC TGCTGGCCAC CTGCTCAGTG 
CACGGAGACC CACCAGGAGC CACCGCCGAG GGCCTCTACT GGACCCTCAA CGGGCGCCGC 
CTGCCCCCTG AGCTCTCCCG TGTACTCAAC GCCTCCACCT TGGCTCTGGC CCTGGCCAAC 
CTCAATGGGT CCAGGCAGCG GTCGGGGGAC AACCTCGTGT GCCACGCCCG TGACGGCAGC 
ATCCTGGCTG GCTCCTGCCT CTATGTTGGC CTGCCCCCAG AGAAACCCGT CAACATCAGC 
TGCTGGTCCA AGAACATGAA GGACTTGACC TGCCGCTGGA CGCCAGGGGC CCACGGGGAG 
ACCTTCCTCC ACACCAACTA CTCCCTCAAG TACAAGCTTA GGTGGTATGG CCAGGACAAC 
ACATGTGAGG AGTACCACAC AGTGGGGCCC CACTCCTGCC ACATCCCCAA GGACCTGGCT 
CTCTTTACGC CCTATGAGAT CTGGGTGGAG GCCACCAACC GCCTGGGCTC TGCCCGCTCC 
GATGTACTCA CGCTGGATAT CCTGGATGTG GTGACCACGG ACCCCCCGCC CGACGTGCAC 
GTGAGCCGCG TCGGGGGCCT GGAGGACCAG CTGAGCGTGC GCTGGGTGTC GCCACCCGCC 
CTCAAGGATT TCCTCTTTCA AGCCAAATAC CAGATCCGCT ACCGAGTGGA GGACAGTGTG 
GACTGGAAGG TGGTGGACGA TGTGAGCAAC CAGACCTCCT GCCGCCTGGC CGGCCTGAAA 
CCCGGCACCG TGTACTTCGT GCAAGTGCGC TGCAACCCCT TTGGCATCTA TGGCTCCAAG 
AAAGCCGGGA TCTGGAGTGA GTGGAGCCAC CCCACAGCCG CCTCCACTCC CCGCAGTGAG 
CGCCCGGGCC CGGGCGGCGG GGCGTGCGAA CCGCGGGGCG GAGAGCCGAG CTCGGGGCCG 
GTGCGGCGCG AGCTCAAGCA GTTCCTGGGC TGGCTCAAGA AGCACGCGTA CTGCTCCAAC 
CTCAGCTTCC GCCTCTACGA CCAGTGGCGA GCCTGGATGC AGAAGTCGCA CAAGACCCGC 
AACCAGCACA GGACGAGGGG ATCCTGCCCT CGGGCAGACG GGGCACGGCG AGAGCCTAAG 
AACATTCAAT GCCCTGAAAA TGTCCGAAGT GGAATCTCCT CCGAAATGAA TGAACATACT 
CACCCTAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRLF1 ( XM_011528423.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526725.1 Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu62658
Clone ID Related Accession (Same CDS sequence)	XM_011528423.2
Accession Version	XM_011528423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	cytokine receptor-like factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGCCG GCCGCCGGGG CCCCGCCGCC CAATCCGCGC GGCGGCCGCC GCCGTTGCTG 
CCCCTGCTGC TGCTGCTCTG CGTCCTCGGG GCGCCGCGAG CCGGATCAGG AGCCCACACA 
GCTGTGATCA GTCCCCAGGA TCCCACGCTT CTCATCGGCT CCTCCCTGCT GGCCACCTGC 
TCAGTGCACG GAGACCCACC AGGAGCCACC GCCGAGGGCC TCTACTGGAC CCTCAACGGG 
CGCCGCCTGC CCCCTGAGCT CTCCCGTGTA CTCAACGCCT CCACCTTGGC TCTGGCCCTG 
GCCAACCTCA ATGGGTCCAG GCAGCGGTCG GGGGACAACC TCGTGTGCCA CGCCCGTGAC 
GGCAGCATCC TGGCTGGCTC CTGCCTCTAT GTTGGCCTGC CCCCAGAGAA ACCCGTCAAC 
ATCAGCTGCT GGTCCAAGAA CATGAAGGAC TTGACCTGCC GCTGGACGCC AGGGGCCCAC 
GGGGAGACCT TCCTCCACAC CAACTACTCC CTCAAGTACA AGCTTAGGTG GTATGGCCAG 
GACAACACAT GTGAGGAGTA CCACACAGTG GGGCCCCACT CCTGCCACAT CCCCAAGGAC 
CTGGCTCTCT TTACGCCCTA TGAGATCTGG GTGGAGGCCA CCAACCGCCT GGGCTCTGCC 
CGCTCCGATG TACTCACGCT GGATATCCTG GATGTGGTGA CCACGGACCC CCCGCCCGAC 
GTGCACGTGA GCCGCGTCGG GGGCCTGGAG GACCAGCTGA GCGTGCGCTG GGTGTCGCCA 
CCCGCCCTCA AGGATTTCCT CTTTCAAGCC AAATACCAGA TCCGCTACCG AGTGGAGGAC 
AGTGTGGACT GGAAGGTGGT GGACGATGTG AGCAACCAGA CCTCCTGCCG CCTGGCCGGC 
CTGAAACCCG GCACCGTGTA CTTCGTGCAA GTGCGCTGCA ACCCCTTTGG CATCTATGGC 
TCCAAGAAAG CCGGGATCTG GAGTGAGTGG AGCCACCCCA CAGCCGCCTC CACTCCCCGC 
AGTGAGCGCC CGGGCCCGGG CGGCGGGGCG TGCGAACCGC GGGGCGGAGA GCCGAGCTCG 
GGGCCGGTGC GGCGCGAGCT CAAGCAGTTC CTGGGCTGGC TCAAGAAGCA CGCGTACTGC 
TCCAACCTCA GCTTCCGCCT CTACGACCAG TGGCGAGCCT GGATGCAGAA GTCGCACAAG 
ACCCGCAACC AGGACGAGGG GATCCTGCCC TCGGGCAGAC GGGGCACGGC GAGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526725.1
CDS	1..1260
Translation	MPAGRRGPAAQSARRPPPLLPLLLLLCVLGAPRAGSGAHTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQDEGILPSGRRGTARA

Target ORF information:

RefSeq Version	XM_011528423.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528423.2

ORF Insert Sequence:

ATGCCCGCCG GCCGCCGGGG CCCCGCCGCC CAATCCGCGC GGCGGCCGCC GCCGTTGCTG 
CCCCTGCTGC TGCTGCTCTG CGTCCTCGGG GCGCCGCGAG CCGGATCAGG AGCCCACACA 
GCTGTGATCA GTCCCCAGGA TCCCACGCTT CTCATCGGCT CCTCCCTGCT GGCCACCTGC 
TCAGTGCACG GAGACCCACC AGGAGCCACC GCCGAGGGCC TCTACTGGAC CCTCAACGGG 
CGCCGCCTGC CCCCTGAGCT CTCCCGTGTA CTCAACGCCT CCACCTTGGC TCTGGCCCTG 
GCCAACCTCA ATGGGTCCAG GCAGCGGTCG GGGGACAACC TCGTGTGCCA CGCCCGTGAC 
GGCAGCATCC TGGCTGGCTC CTGCCTCTAT GTTGGCCTGC CCCCAGAGAA ACCCGTCAAC 
ATCAGCTGCT GGTCCAAGAA CATGAAGGAC TTGACCTGCC GCTGGACGCC AGGGGCCCAC 
GGGGAGACCT TCCTCCACAC CAACTACTCC CTCAAGTACA AGCTTAGGTG GTATGGCCAG 
GACAACACAT GTGAGGAGTA CCACACAGTG GGGCCCCACT CCTGCCACAT CCCCAAGGAC 
CTGGCTCTCT TTACGCCCTA TGAGATCTGG GTGGAGGCCA CCAACCGCCT GGGCTCTGCC 
CGCTCCGATG TACTCACGCT GGATATCCTG GATGTGGTGA CCACGGACCC CCCGCCCGAC 
GTGCACGTGA GCCGCGTCGG GGGCCTGGAG GACCAGCTGA GCGTGCGCTG GGTGTCGCCA 
CCCGCCCTCA AGGATTTCCT CTTTCAAGCC AAATACCAGA TCCGCTACCG AGTGGAGGAC 
AGTGTGGACT GGAAGGTGGT GGACGATGTG AGCAACCAGA CCTCCTGCCG CCTGGCCGGC 
CTGAAACCCG GCACCGTGTA CTTCGTGCAA GTGCGCTGCA ACCCCTTTGG CATCTATGGC 
TCCAAGAAAG CCGGGATCTG GAGTGAGTGG AGCCACCCCA CAGCCGCCTC CACTCCCCGC 
AGTGAGCGCC CGGGCCCGGG CGGCGGGGCG TGCGAACCGC GGGGCGGAGA GCCGAGCTCG 
GGGCCGGTGC GGCGCGAGCT CAAGCAGTTC CTGGGCTGGC TCAAGAAGCA CGCGTACTGC 
TCCAACCTCA GCTTCCGCCT CTACGACCAG TGGCGAGCCT GGATGCAGAA GTCGCACAAG 
ACCCGCAACC AGGACGAGGG GATCCTGCCC TCGGGCAGAC GGGGCACGGC GAGAGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRLF1 ( XM_011528424.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526726.1 Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu62659
Clone ID Related Accession (Same CDS sequence)	XM_011528424.2 , XM_011528424.3
Accession Version	XM_011528424.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cytokine receptor-like factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528424.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGGACGTGCA GTTTGCCAGG GCTGCCTTTA GTTCCAGAGA CACAGCTGTG 
ATCAGTCCCC AGGATCCCAC GCTTCTCATC GGCTCCTCCC TGCTGGCCAC CTGCTCAGTG 
CACGGAGACC CACCAGGAGC CACCGCCGAG GGCCTCTACT GGACCCTCAA CGGGCGCCGC 
CTGCCCCCTG AGCTCTCCCG TGTACTCAAC GCCTCCACCT TGGCTCTGGC CCTGGCCAAC 
CTCAATGGGT CCAGGCAGCG GTCGGGGGAC AACCTCGTGT GCCACGCCCG TGACGGCAGC 
ATCCTGGCTG GCTCCTGCCT CTATGTTGGC CTGCCCCCAG AGAAACCCGT CAACATCAGC 
TGCTGGTCCA AGAACATGAA GGACTTGACC TGCCGCTGGA CGCCAGGGGC CCACGGGGAG 
ACCTTCCTCC ACACCAACTA CTCCCTCAAG TACAAGCTTA GGTGGTATGG CCAGGACAAC 
ACATGTGAGG AGTACCACAC AGTGGGGCCC CACTCCTGCC ACATCCCCAA GGACCTGGCT 
CTCTTTACGC CCTATGAGAT CTGGGTGGAG GCCACCAACC GCCTGGGCTC TGCCCGCTCC 
GATGTACTCA CGCTGGATAT CCTGGATGTG GTGACCACGG ACCCCCCGCC CGACGTGCAC 
GTGAGCCGCG TCGGGGGCCT GGAGGACCAG CTGAGCGTGC GCTGGGTGTC GCCACCCGCC 
CTCAAGGATT TCCTCTTTCA AGCCAAATAC CAGATCCGCT ACCGAGTGGA GGACAGTGTG 
GACTGGAAGG TGGTGGACGA TGTGAGCAAC CAGACCTCCT GCCGCCTGGC CGGCCTGAAA 
CCCGGCACCG TGTACTTCGT GCAAGTGCGC TGCAACCCCT TTGGCATCTA TGGCTCCAAG 
AAAGCCGGGA TCTGGAGTGA GTGGAGCCAC CCCACAGCCG CCTCCACTCC CCGCAGTGAG 
CGCCCGGGCC CGGGCGGCGG GGCGTGCGAA CCGCGGGGCG GAGAGCCGAG CTCGGGGCCG 
GTGCGGCGCG AGCTCAAGCA GTTCCTGGGC TGGCTCAAGA AGCACGCGTA CTGCTCCAAC 
CTCAGCTTCC GCCTCTACGA CCAGTGGCGA GCCTGGATGC AGAAGTCGCA CAAGACCCGC 
AACCAGCACA GGACGAGGGG ATCCTGCCCT CGGGCAGACG GGGCACGGCG AGAGGTCCTG 
CCAGATAAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526726.1
CDS	335..1549
Translation	MGVGDVQFARAAFSSRDTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQHRTRGSCPRADGARREVLPDKL

Target ORF information:

RefSeq Version	XM_011528424.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528424.2

ORF Insert Sequence:

ATGGGAGTTG GGGACGTGCA GTTTGCCAGG GCTGCCTTTA GTTCCAGAGA CACAGCTGTG 
ATCAGTCCCC AGGATCCCAC GCTTCTCATC GGCTCCTCCC TGCTGGCCAC CTGCTCAGTG 
CACGGAGACC CACCAGGAGC CACCGCCGAG GGCCTCTACT GGACCCTCAA CGGGCGCCGC 
CTGCCCCCTG AGCTCTCCCG TGTACTCAAC GCCTCCACCT TGGCTCTGGC CCTGGCCAAC 
CTCAATGGGT CCAGGCAGCG GTCGGGGGAC AACCTCGTGT GCCACGCCCG TGACGGCAGC 
ATCCTGGCTG GCTCCTGCCT CTATGTTGGC CTGCCCCCAG AGAAACCCGT CAACATCAGC 
TGCTGGTCCA AGAACATGAA GGACTTGACC TGCCGCTGGA CGCCAGGGGC CCACGGGGAG 
ACCTTCCTCC ACACCAACTA CTCCCTCAAG TACAAGCTTA GGTGGTATGG CCAGGACAAC 
ACATGTGAGG AGTACCACAC AGTGGGGCCC CACTCCTGCC ACATCCCCAA GGACCTGGCT 
CTCTTTACGC CCTATGAGAT CTGGGTGGAG GCCACCAACC GCCTGGGCTC TGCCCGCTCC 
GATGTACTCA CGCTGGATAT CCTGGATGTG GTGACCACGG ACCCCCCGCC CGACGTGCAC 
GTGAGCCGCG TCGGGGGCCT GGAGGACCAG CTGAGCGTGC GCTGGGTGTC GCCACCCGCC 
CTCAAGGATT TCCTCTTTCA AGCCAAATAC CAGATCCGCT ACCGAGTGGA GGACAGTGTG 
GACTGGAAGG TGGTGGACGA TGTGAGCAAC CAGACCTCCT GCCGCCTGGC CGGCCTGAAA 
CCCGGCACCG TGTACTTCGT GCAAGTGCGC TGCAACCCCT TTGGCATCTA TGGCTCCAAG 
AAAGCCGGGA TCTGGAGTGA GTGGAGCCAC CCCACAGCCG CCTCCACTCC CCGCAGTGAG 
CGCCCGGGCC CGGGCGGCGG GGCGTGCGAA CCGCGGGGCG GAGAGCCGAG CTCGGGGCCG 
GTGCGGCGCG AGCTCAAGCA GTTCCTGGGC TGGCTCAAGA AGCACGCGTA CTGCTCCAAC 
CTCAGCTTCC GCCTCTACGA CCAGTGGCGA GCCTGGATGC AGAAGTCGCA CAAGACCCGC 
AACCAGCACA GGACGAGGGG ATCCTGCCCT CGGGCAGACG GGGCACGGCG AGAGGTCCTG 
CCAGATAAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CRLF1 ( XM_011528424.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526726.1 Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu62659
Clone ID Related Accession (Same CDS sequence)	XM_011528424.2 , XM_011528424.3
Accession Version	XM_011528424.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	cytokine receptor-like factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528424.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGTTG GGGACGTGCA GTTTGCCAGG GCTGCCTTTA GTTCCAGAGA CACAGCTGTG 
ATCAGTCCCC AGGATCCCAC GCTTCTCATC GGCTCCTCCC TGCTGGCCAC CTGCTCAGTG 
CACGGAGACC CACCAGGAGC CACCGCCGAG GGCCTCTACT GGACCCTCAA CGGGCGCCGC 
CTGCCCCCTG AGCTCTCCCG TGTACTCAAC GCCTCCACCT TGGCTCTGGC CCTGGCCAAC 
CTCAATGGGT CCAGGCAGCG GTCGGGGGAC AACCTCGTGT GCCACGCCCG TGACGGCAGC 
ATCCTGGCTG GCTCCTGCCT CTATGTTGGC CTGCCCCCAG AGAAACCCGT CAACATCAGC 
TGCTGGTCCA AGAACATGAA GGACTTGACC TGCCGCTGGA CGCCAGGGGC CCACGGGGAG 
ACCTTCCTCC ACACCAACTA CTCCCTCAAG TACAAGCTTA GGTGGTATGG CCAGGACAAC 
ACATGTGAGG AGTACCACAC AGTGGGGCCC CACTCCTGCC ACATCCCCAA GGACCTGGCT 
CTCTTTACGC CCTATGAGAT CTGGGTGGAG GCCACCAACC GCCTGGGCTC TGCCCGCTCC 
GATGTACTCA CGCTGGATAT CCTGGATGTG GTGACCACGG ACCCCCCGCC CGACGTGCAC 
GTGAGCCGCG TCGGGGGCCT GGAGGACCAG CTGAGCGTGC GCTGGGTGTC GCCACCCGCC 
CTCAAGGATT TCCTCTTTCA AGCCAAATAC CAGATCCGCT ACCGAGTGGA GGACAGTGTG 
GACTGGAAGG TGGTGGACGA TGTGAGCAAC CAGACCTCCT GCCGCCTGGC CGGCCTGAAA 
CCCGGCACCG TGTACTTCGT GCAAGTGCGC TGCAACCCCT TTGGCATCTA TGGCTCCAAG 
AAAGCCGGGA TCTGGAGTGA GTGGAGCCAC CCCACAGCCG CCTCCACTCC CCGCAGTGAG 
CGCCCGGGCC CGGGCGGCGG GGCGTGCGAA CCGCGGGGCG GAGAGCCGAG CTCGGGGCCG 
GTGCGGCGCG AGCTCAAGCA GTTCCTGGGC TGGCTCAAGA AGCACGCGTA CTGCTCCAAC 
CTCAGCTTCC GCCTCTACGA CCAGTGGCGA GCCTGGATGC AGAAGTCGCA CAAGACCCGC 
AACCAGCACA GGACGAGGGG ATCCTGCCCT CGGGCAGACG GGGCACGGCG AGAGGTCCTG 
CCAGATAAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526726.1
CDS	336..1550
Translation	MGVGDVQFARAAFSSRDTAVISPQDPTLLIGSSLLATCSVHGDPPGATAEGLYWTLNGRRLPPELSRVLNASTLALALANLNGSRQRSGDNLVCHARDGSILAGSCLYVGLPPEKPVNISCWSKNMKDLTCRWTPGAHGETFLHTNYSLKYKLRWYGQDNTCEEYHTVGPHSCHIPKDLALFTPYEIWVEATNRLGSARSDVLTLDILDVVTTDPPPDVHVSRVGGLEDQLSVRWVSPPALKDFLFQAKYQIRYRVEDSVDWKVVDDVSNQTSCRLAGLKPGTVYFVQVRCNPFGIYGSKKAGIWSEWSHPTAASTPRSERPGPGGGACEPRGGEPSSGPVRRELKQFLGWLKKHAYCSNLSFRLYDQWRAWMQKSHKTRNQHRTRGSCPRADGARREVLPDKL

Target ORF information:

RefSeq Version	XM_011528424.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytokine receptor like factor 1 (CRLF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528424.3

ORF Insert Sequence:

ATGGGAGTTG GGGACGTGCA GTTTGCCAGG GCTGCCTTTA GTTCCAGAGA CACAGCTGTG 
ATCAGTCCCC AGGATCCCAC GCTTCTCATC GGCTCCTCCC TGCTGGCCAC CTGCTCAGTG 
CACGGAGACC CACCAGGAGC CACCGCCGAG GGCCTCTACT GGACCCTCAA CGGGCGCCGC 
CTGCCCCCTG AGCTCTCCCG TGTACTCAAC GCCTCCACCT TGGCTCTGGC CCTGGCCAAC 
CTCAATGGGT CCAGGCAGCG GTCGGGGGAC AACCTCGTGT GCCACGCCCG TGACGGCAGC 
ATCCTGGCTG GCTCCTGCCT CTATGTTGGC CTGCCCCCAG AGAAACCCGT CAACATCAGC 
TGCTGGTCCA AGAACATGAA GGACTTGACC TGCCGCTGGA CGCCAGGGGC CCACGGGGAG 
ACCTTCCTCC ACACCAACTA CTCCCTCAAG TACAAGCTTA GGTGGTATGG CCAGGACAAC 
ACATGTGAGG AGTACCACAC AGTGGGGCCC CACTCCTGCC ACATCCCCAA GGACCTGGCT 
CTCTTTACGC CCTATGAGAT CTGGGTGGAG GCCACCAACC GCCTGGGCTC TGCCCGCTCC 
GATGTACTCA CGCTGGATAT CCTGGATGTG GTGACCACGG ACCCCCCGCC CGACGTGCAC 
GTGAGCCGCG TCGGGGGCCT GGAGGACCAG CTGAGCGTGC GCTGGGTGTC GCCACCCGCC 
CTCAAGGATT TCCTCTTTCA AGCCAAATAC CAGATCCGCT ACCGAGTGGA GGACAGTGTG 
GACTGGAAGG TGGTGGACGA TGTGAGCAAC CAGACCTCCT GCCGCCTGGC CGGCCTGAAA 
CCCGGCACCG TGTACTTCGT GCAAGTGCGC TGCAACCCCT TTGGCATCTA TGGCTCCAAG 
AAAGCCGGGA TCTGGAGTGA GTGGAGCCAC CCCACAGCCG CCTCCACTCC CCGCAGTGAG 
CGCCCGGGCC CGGGCGGCGG GGCGTGCGAA CCGCGGGGCG GAGAGCCGAG CTCGGGGCCG 
GTGCGGCGCG AGCTCAAGCA GTTCCTGGGC TGGCTCAAGA AGCACGCGTA CTGCTCCAAC 
CTCAGCTTCC GCCTCTACGA CCAGTGGCGA GCCTGGATGC AGAAGTCGCA CAAGACCCGC 
AACCAGCACA GGACGAGGGG ATCCTGCCCT CGGGCAGACG GGGCACGGCG AGAGGTCCTG 
CCAGATAAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutations in CRLF1 cause familial achalasia.
Clinical genetics92(1)104-108(2017 Jul)
Busch A,?arkovi? M,Lowe C,Jankofsky M,Ganschow R,Buers I,Kurth I,Reutter H,Rutsch F,H?bner CA

Crisponi/CISS1 syndrome: A case series.
American journal of medical genetics. Part A170A(5)1236-41(2016 May)
Alhashem AM,Majeed-Saidan MA,Ammari AN,Alrakaf MS,Nojoom M,Maddirevula S,Faqeih E,Alkuraya FS,Garne E,Kurdi AM

Cytokine-Like Factor 1, an Essential Facilitator of Cardiotrophin-Like Cytokine:Ciliary Neurotrophic Factor Receptor ? Signaling and sorLA-Mediated Turnover.
Molecular and cellular biology36(8)1272-86(2016 Apr)
Larsen JV,Kristensen AM,Pallesen LT,Bauer J,V?gter CB,Nielsen MS,Madsen P,Petersen CM

Expanding the mutational spectrum of CRLF1 in Crisponi/CISS1 syndrome.
Human mutation35(4)424-33(2014 Apr)
Piras R,Chiappe F,Torraca IL,Buers I,Usala G,Angius A,Akin MA,Basel-Vanagaite L,Benedicenti F,Chiodin E,El Assy O,Feingold-Zadok M,Guibert J,Kamien B,Kasapkara CS,Kili? E,Boduro?lu K,Kurtoglu S,Manzur AY,Onal EE,Paderi E,Roche CH,T?mer L,Unal S,Utine GE,Zanda G,Zankl A,Zampino G,Crisponi G,Crisponi L,Rutsch F

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CRLF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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