MRRF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRRF
Entrez Gene ID	92399
Full Name	mitochondrial ribosome recycling factor
Synonyms	MRFF,MTRRF,RRF
General protein information	Preferred Names mitochondrial ribosome recycling factor Names ribosome-recycling factor, mitochondrial ribosome-releasing factor, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q33.2
Summary	This gene encodes a ribosome recycling factor, which is a component of the mitochondrial translational machinery. The encoded protein, along with mitochondrial elongation factor 2, functions in ribosomal recycling at the termination of mitochondrial translation by mediating the disassembly of ribosomes from messenger RNA. A pseudogene of this gene has been identified on chromosome X. [provided by RefSeq, Oct 2016].
Disorder MIM:	604602

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346339.1	NP_001333268.1	ribosome-recycling factor, mitochondrial isoform 4
NM_001346347.2	NP_001333276.1	ribosome-recycling factor, mitochondrial isoform 7
XM_011519183.2	XP_011517485.1	ribosome-recycling factor, mitochondrial isoform X1
NM_138777.3	NP_620132.1	ribosome-recycling factor, mitochondrial isoform 1 precursor
NM_138777.4	NP_620132.1	ribosome-recycling factor, mitochondrial isoform 1 precursor
XM_011519184.1	XP_011517486.1	ribosome-recycling factor, mitochondrial isoform X1
XM_011519185.2	XP_011517487.1	ribosome-recycling factor, mitochondrial isoform X3
NM_199177.2	NP_954646.1	ribosome-recycling factor, mitochondrial isoform 2 precursor
NM_001346345.1	NP_001333274.1	ribosome-recycling factor, mitochondrial isoform 6
NM_001346341.2	NP_001333270.1	ribosome-recycling factor, mitochondrial isoform 1 precursor
NM_199177.3	NP_954646.1	ribosome-recycling factor, mitochondrial isoform 2 precursor
XM_017015286.1	XP_016870775.1	ribosome-recycling factor, mitochondrial isoform X4
NM_138777.5	NP_620132.1	ribosome-recycling factor, mitochondrial isoform 1 precursor
NM_001173512.2	NP_001166983.1	ribosome-recycling factor, mitochondrial isoform 3 precursor
XM_017015286.2	XP_016870775.1	ribosome-recycling factor, mitochondrial isoform X3
NM_001346347.1	NP_001333276.1	ribosome-recycling factor, mitochondrial isoform 7
XM_011519186.1	XP_011517488.1	ribosome-recycling factor, mitochondrial isoform X2
NM_001346343.1	NP_001333272.1	ribosome-recycling factor, mitochondrial isoform 5
NM_001346341.1	NP_001333270.1	ribosome-recycling factor, mitochondrial isoform 1 precursor
NM_001346345.2	NP_001333274.1	ribosome-recycling factor, mitochondrial isoform 6
NM_001346339.2	NP_001333268.1	ribosome-recycling factor, mitochondrial isoform 4
NM_001346343.2	NP_001333272.1	ribosome-recycling factor, mitochondrial isoform 5
NM_199177.4	NP_954646.1	ribosome-recycling factor, mitochondrial isoform 2 precursor
XM_011519185.3	XP_011517487.1	ribosome-recycling factor, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MRRF	XP_415395.1
Danio rerio (zebrafish)	mrrf	NP_001002174.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006804	XP_308944.2
	MRRF	XP_001089220.2
Drosophila melanogaster (fruit fly)	CG4447	NP_648302.2
Pan troglodytes (chimpanzee)	MRRF	XP_520237.3
Mus musculus (house mouse)	Mrrf	NP_080698.1
Homo sapiens (human)	MRRF	NP_620132.1
Canis lupus familiaris (dog)	MRRF	XP_005625460.1
Bos taurus (cattle)	MRRF	NP_001069775.1
Xenopus tropicalis (tropical clawed frog)	mrrf	NP_001072392.1
Rattus norvegicus (Norway rat)	Mrrf	NP_001008355.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis.
Tsuboi M, Morita H, Nozaki Y, Akama K, Ueda T, Ito K, Nierhaus KH, Takeuchi N
Molecular cell35(4)502-10(2009 Aug)

The human mitochondrial ribosome recycling factor is essential for cell viability.
Rorbach J, Richter R, Wessels HJ, Wydro M, Pekalski M, Farhoud M, K?hl I, Gaisne M, Bonnefoy N, Smeitink JA, Lightowlers RN, Chrzanowska-Lightowlers ZM
Nucleic acids research36(18)5787-99(2008 Oct)

DNA sequence and analysis of human chromosome 9.
Humphray SJ, Oliver K, Hunt AR, Plumb RW, Loveland JE, Howe KL, Andrews TD, Searle S, Hunt SE, Scott CE, Jones MC, Ainscough R, Almeida JP, Ambrose KD, Ashwell RI, Babbage AK, Babbage S, Bagguley CL, Bailey J, Banerjee R, Barker DJ, Barlow KF, Bates K, Beasley H, Beasley O, Bird CP, Bray-Allen S, Brown AJ, Brown JY, Burford D, Burrill W, Burton J, Carder C, Carter NP, Chapman JC, Chen Y, Clarke G, Clark SY, Clee CM, Clegg S, Collier RE, Corby N, Crosier M, Cummings AT, Davies J, Dhami P, Dunn M, Dutta I, Dyer LW, Earthrowl ME, Faulkner L, Fleming CJ, Frankish A, Frankland JA, French L, Fricker DG, Garner P, Garnett J, Ghori J, Gilbert JG, Glison C, Grafham DV, Gribble S, Griffiths C, Griffiths-Jones S, Grocock R, Guy J, Hall RE, Hammond S, Harley JL, Harrison ES, Hart EA, Heath PD, Henderson CD, Hopkins BL, Howard PJ, Howden PJ, Huckle E, Johnson C, Johnson D, Joy AA, Kay M, Keenan S, Kershaw JK, Kimberley AM, King A, Knights A, Laird GK, Langford C, Lawlor S, Leongamornlert DA, Leversha M, Lloyd C, Lloyd DM, Lovell J, Martin S, Mashreghi-Mohammadi M, Matthews L, McLaren S, McLay KE, McMurray A, Milne S, Nickerson T, Nisbett J, Nordsiek G, Pearce AV, Peck AI, Porter KM, Pandian R, Pelan S, Phillimore B, Povey S, Ramsey Y, Rand V, Scharfe M, Sehra HK, Shownkeen R, Sims SK, Skuce CD, Smith M, Steward CA, Swarbreck D, Sycamore N, Tester J, Thorpe A, Tracey A, Tromans A, Thomas DW, Wall M, Wallis JM, West AP, Whitehead SL, Willey DL, Williams SA, Wilming L, Wray PW, Young L, Ashurst JL, Coulson A, Bl?cker H, Durbin R, Sulston JE, Hubbard T, Jackson MJ, Bentley DR, Beck S, Rogers J, Dunham I
Nature429(6990)369-74(2004 May)

Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping.
Hansen LL, J?rgensen R, Justesen J
Cytogenetics and cell genetics88(1-2)91-2(2000)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis.
Title: EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis.

Depletion of mtRRF in human cell lines is lethal, initially causing profound mitochondrial dysmorphism, aggregation of mitoribosomes and elevated mitochondrial superoxide production.
Title: The human mitochondrial ribosome recycling factor is essential for cell viability.

The following MRRF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRRF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08482	NM_138777.5 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08482	NM_001346341.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09450	NM_199177.4 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08482	XM_011519184.1 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09450	XM_011519186.1 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu74479	NM_001346347.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu07672	NM_001173512.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu102350	NM_001346343.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu43581	NM_001346339.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu43581	XM_011519183.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu74478	XM_011519185.3 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu102262	NM_001346345.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu98998	XM_017015286.2 Latest version!	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu08482	NM_138777.3	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu09450	NM_199177.2	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu08482	NM_138777.4	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu98998	XM_017015286.1	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu74479	NM_001346347.1	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu08482	NM_001346341.1	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu102262	NM_001346345.1	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu43581	NM_001346339.1	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu102350	NM_001346343.1	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu09450	NM_199177.3	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu74478	XM_011519185.2	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRRF ( NM_138777.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_620132.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08482
Clone ID Related Accession (Same CDS sequence)	NM_138777.3 , NM_138777.4 , XM_011519184.1 , NM_001346341.2 , NM_138777.5 , NM_001346341.1
Accession Version	NM_138777.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	ribosome-recycling factor, mitochondrial isoform 1 precursor
Comment

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620132.1
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_138777.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138777.3

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_138777.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_620132.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08482
Clone ID Related Accession (Same CDS sequence)	NM_138777.3 , NM_138777.4 , XM_011519184.1 , NM_001346341.2 , NM_138777.5 , NM_001346341.1
Accession Version	NM_138777.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-09
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310974.1, AL162424.20, AK291603.1, BC013049.1 and AA873020.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2016 this sequence version replaced NM_138777.3. Transcript Variant: Variants 1 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.222026.1, SRR1163655.442803.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620132.1
CDS	365..1153
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_138777.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138777.4

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346341.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333270.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu08482
Clone ID Related Accession (Same CDS sequence)	NM_138777.3 , NM_138777.4 , XM_011519184.1 , NM_001346341.2 , NM_138777.5 , NM_001346341.1
Accession Version	NM_001346341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On May 31, 2019 this sequence version replaced NM_001346341.1. Transcript Variant: Variants 1 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.591260.1, SRR5189661.102573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333270.1
CDS	170..958
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346341.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346341.2

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_138777.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_620132.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.

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CloneID	OHu08482
Clone ID Related Accession (Same CDS sequence)	NM_138777.3 , NM_138777.4 , XM_011519184.1 , NM_001346341.2 , NM_138777.5 , NM_001346341.1
Accession Version	NM_138777.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20, AK291603.1, BC013049.1 and AA873020.1. On Nov 23, 2018 this sequence version replaced NM_138777.4. Transcript Variant: Variants 1 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.331392.1, SRR3476690.50162.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000344641.8/ ENSP00000343867.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620132.1
CDS	86..874
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_138777.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138777.5

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346341.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333270.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 5, mRNA.

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CloneID	OHu08482
Clone ID Related Accession (Same CDS sequence)	NM_138777.3 , NM_138777.4 , XM_011519184.1 , NM_001346341.2 , NM_138777.5 , NM_001346341.1
Accession Version	NM_001346341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On Oct 7, 2016 this sequence version replaced XM_011519184.2. Transcript Variant: Variants 1 and 5 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.712163.1, SRR1163657.224332.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333270.1
CDS	449..1237
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346341.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346341.1

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_199177.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_954646.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09450
Clone ID Related Accession (Same CDS sequence)	NM_199177.2 , NM_199177.3 , XM_011519186.1 , NM_199177.4
Accession Version	NM_199177.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	ribosome-recycling factor, mitochondrial isoform 2 precursor
Comment

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954646.1
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ

Target ORF information:

RefSeq Version	NM_199177.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199177.2

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_199177.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_954646.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09450
Clone ID Related Accession (Same CDS sequence)	NM_199177.2 , NM_199177.3 , XM_011519186.1 , NM_199177.4
Accession Version	NM_199177.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310974.1, AL162424.20, BU543762.1, AK291603.1, BC053604.1, BC013049.1 and AA873020.1. On or before Oct 7, 2016 this sequence version replaced XM_011519186.1, NM_199177.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.456186.1, BU543762.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954646.1
CDS	365..970
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ

Target ORF information:

RefSeq Version	NM_199177.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199177.3

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_199177.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_954646.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09450
Clone ID Related Accession (Same CDS sequence)	NM_199177.2 , NM_199177.3 , XM_011519186.1 , NM_199177.4
Accession Version	NM_199177.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20, BU543762.1, AK291603.1, BC053604.1, BC013049.1 and AA873020.1. On May 31, 2019 this sequence version replaced NM_199177.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.943002.1, SRR1163655.456186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954646.1
CDS	86..691
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ

Target ORF information:

RefSeq Version	NM_199177.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199177.4

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_011519184.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517486.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X3, mRNA.

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CloneID	OHu08482
Clone ID Related Accession (Same CDS sequence)	NM_138777.3 , NM_138777.4 , XM_011519184.1 , NM_001346341.2 , NM_138777.5 , NM_001346341.1
Accession Version	XM_011519184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	ribosome-recycling factor, mitochondrial isoform X1
Comment

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517486.1
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	XM_011519184.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519184.1

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AAGTAACCAG AGAGCACAGA GAAATGCTGG TGAAACTGGC CAAACAGAAC 
ACCAACAAGG CCAAAGACTC TTTACGGAAG GTTCGCACCA ACTCAATGAA CAAGCTGAAG 
AAATCCAAGG ATACAGTCTC AGAGGACACC ATTAGGCTAA TAGAGAAACA GATCAGCCAA 
ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG CAGTGAAGAC CAAAGAACTC 
CTTGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_011519186.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517488.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X5, mRNA.

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CloneID	OHu09450
Clone ID Related Accession (Same CDS sequence)	NM_199177.2 , NM_199177.3 , XM_011519186.1 , NM_199177.4
Accession Version	XM_011519186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	ribosome-recycling factor, mitochondrial isoform X2
Comment

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517488.1
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ

Target ORF information:

RefSeq Version	XM_011519186.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519186.1

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGT GTACAGCTGC AGCTATCAAG 
GCTATAAGAG AAAGTGGAAT GAATCTGAAC CCAGAAGTGG AAGGGACGCT AATTCGGGTA 
CCCATTCCCC AATCAGCCAA ATGGCCGATG ACACAGTGGC AGAACTGGAC AGGCATCTGG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346347.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333276.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 8, mRNA.

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CloneID	OHu74479
Clone ID Related Accession (Same CDS sequence)	NM_001346347.2 , NM_001346347.1
Accession Version	NM_001346347.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On May 31, 2019 this sequence version replaced NM_001346347.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.99592.1, SRR5189664.67944.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGTCTG TGATAGAAGC TCTCAAGGAT AATTTCAATA AGACTCTCAA TATAAGGACC 
TCACCAGGAT CCCTTGACAA GATTGCTGTG GTAACTGCTG ACGGGAAGCT TGCTTTAAAC 
CAGATTAGCC AGATCTCCAT GAAGTCGCCA CAGCTGATTT TGGTGAATAT GGCCAGCTTC 
CCAGAGTGTA CAGCTGCAGC TATCAAGGCT ATAAGAGAAA GTGGAATGAA TCTGAACCCA 
GAAGTGGAAG GGACGCTAAT TCGGGTACCC ATTCCCCAAG TAACCAGAGA GCACAGAGAA 
ATGCTGGTGA AACTGGCCAA ACAGAACACC AACAAGGCCA AAGACTCTTT ACGGAAGGTT 
CGCACCAACT CAATGAACAA GCTGAAGAAA TCCAAGGATA CAGTCTCAGA GGACACCATT 
AGGCTAATAG AGAAACAGAT CAGCCAAATG GCCGATGACA CAGTGGCAGA ACTGGACAGG 
CATCTGGCAG TGAAGACCAA AGAACTCCTT GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333276.1
CDS	147..662
Translation	MKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346347.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346347.2

ORF Insert Sequence:

ATGAAGTCTG TGATAGAAGC TCTCAAGGAT AATTTCAATA AGACTCTCAA TATAAGGACC 
TCACCAGGAT CCCTTGACAA GATTGCTGTG GTAACTGCTG ACGGGAAGCT TGCTTTAAAC 
CAGATTAGCC AGATCTCCAT GAAGTCGCCA CAGCTGATTT TGGTGAATAT GGCCAGCTTC 
CCAGAGTGTA CAGCTGCAGC TATCAAGGCT ATAAGAGAAA GTGGAATGAA TCTGAACCCA 
GAAGTGGAAG GGACGCTAAT TCGGGTACCC ATTCCCCAAG TAACCAGAGA GCACAGAGAA 
ATGCTGGTGA AACTGGCCAA ACAGAACACC AACAAGGCCA AAGACTCTTT ACGGAAGGTT 
CGCACCAACT CAATGAACAA GCTGAAGAAA TCCAAGGATA CAGTCTCAGA GGACACCATT 
AGGCTAATAG AGAAACAGAT CAGCCAAATG GCCGATGACA CAGTGGCAGA ACTGGACAGG 
CATCTGGCAG TGAAGACCAA AGAACTCCTT GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346347.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333276.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu74479
Clone ID Related Accession (Same CDS sequence)	NM_001346347.2 , NM_001346347.1
Accession Version	NM_001346347.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On Oct 7, 2016 this sequence version replaced XM_011519187.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.99592.1, SRR1163658.483530.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGTCTG TGATAGAAGC TCTCAAGGAT AATTTCAATA AGACTCTCAA TATAAGGACC 
TCACCAGGAT CCCTTGACAA GATTGCTGTG GTAACTGCTG ACGGGAAGCT TGCTTTAAAC 
CAGATTAGCC AGATCTCCAT GAAGTCGCCA CAGCTGATTT TGGTGAATAT GGCCAGCTTC 
CCAGAGTGTA CAGCTGCAGC TATCAAGGCT ATAAGAGAAA GTGGAATGAA TCTGAACCCA 
GAAGTGGAAG GGACGCTAAT TCGGGTACCC ATTCCCCAAG TAACCAGAGA GCACAGAGAA 
ATGCTGGTGA AACTGGCCAA ACAGAACACC AACAAGGCCA AAGACTCTTT ACGGAAGGTT 
CGCACCAACT CAATGAACAA GCTGAAGAAA TCCAAGGATA CAGTCTCAGA GGACACCATT 
AGGCTAATAG AGAAACAGAT CAGCCAAATG GCCGATGACA CAGTGGCAGA ACTGGACAGG 
CATCTGGCAG TGAAGACCAA AGAACTCCTT GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333276.1
CDS	426..941
Translation	MKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346347.1

ORF Insert Sequence:

ATGAAGTCTG TGATAGAAGC TCTCAAGGAT AATTTCAATA AGACTCTCAA TATAAGGACC 
TCACCAGGAT CCCTTGACAA GATTGCTGTG GTAACTGCTG ACGGGAAGCT TGCTTTAAAC 
CAGATTAGCC AGATCTCCAT GAAGTCGCCA CAGCTGATTT TGGTGAATAT GGCCAGCTTC 
CCAGAGTGTA CAGCTGCAGC TATCAAGGCT ATAAGAGAAA GTGGAATGAA TCTGAACCCA 
GAAGTGGAAG GGACGCTAAT TCGGGTACCC ATTCCCCAAG TAACCAGAGA GCACAGAGAA 
ATGCTGGTGA AACTGGCCAA ACAGAACACC AACAAGGCCA AAGACTCTTT ACGGAAGGTT 
CGCACCAACT CAATGAACAA GCTGAAGAAA TCCAAGGATA CAGTCTCAGA GGACACCATT 
AGGCTAATAG AGAAACAGAT CAGCCAAATG GCCGATGACA CAGTGGCAGA ACTGGACAGG 
CATCTGGCAG TGAAGACCAA AGAACTCCTT GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001173512.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166983.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07672
Clone ID Related Accession (Same CDS sequence)	NM_001173512.2
Accession Version	NM_001173512.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310974.1, AL162424.20, AK300718.1, BC013049.1 and AA873020.1. On Oct 7, 2016 this sequence version replaced NM_001173512.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK300718.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGGATCCC TTGACAAGAT TGCTGTGGTA ACTGCTGACG GGAAGCTTGC TTTAAACCAG 
ATTAGCCAGA TCTCCATGAA GTCGCCACAG CTGATTTTGG TGAATATGGC CAGCTTCCCA 
GAGTGTACAG CTGCAGCTAT CAAGGCTATA AGAGAAAGTG GAATGAATCT GAACCCAGAA 
GTGGAAGGGA CGCTAATTCG GGTACCCATT CCCCAAGTAA CCAGAGAGCA CAGAGAAATG 
CTGGTGAAAC TGGCCAAACA GAACACCAAC AAGGCCAAAG ACTCTTTACG GAAGGTTCGC 
ACCAACTCAA TGAACAAGCT GAAGAAATCC AAGGATACAG TCTCAGAGGA CACCATTAGG 
CTAATAGAGA AACAGATCAG CCAAATGGCC GATGACACAG TGGCAGAACT GGACAGGCAT 
CTGGCAGTGA AGACCAAAGA ACTCCTTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166983.1
CDS	365..997
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001173512.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173512.2

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGGATCCC TTGACAAGAT TGCTGTGGTA ACTGCTGACG GGAAGCTTGC TTTAAACCAG 
ATTAGCCAGA TCTCCATGAA GTCGCCACAG CTGATTTTGG TGAATATGGC CAGCTTCCCA 
GAGTGTACAG CTGCAGCTAT CAAGGCTATA AGAGAAAGTG GAATGAATCT GAACCCAGAA 
GTGGAAGGGA CGCTAATTCG GGTACCCATT CCCCAAGTAA CCAGAGAGCA CAGAGAAATG 
CTGGTGAAAC TGGCCAAACA GAACACCAAC AAGGCCAAAG ACTCTTTACG GAAGGTTCGC 
ACCAACTCAA TGAACAAGCT GAAGAAATCC AAGGATACAG TCTCAGAGGA CACCATTAGG 
CTAATAGAGA AACAGATCAG CCAAATGGCC GATGACACAG TGGCAGAACT GGACAGGCAT 
CTGGCAGTGA AGACCAAAGA ACTCCTTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346343.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333272.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu102350
Clone ID Related Accession (Same CDS sequence)	NM_001346343.1 , NM_001346343.2
Accession Version	NM_001346343.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-14
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK310974.1, AL162424.20, BI756457.1, AK300718.1 and AA873020.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BI756457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2153932 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTATT CAGCTGTACC AGTCCGCCAT TTTGCTACCA AGAAAGCCAA AGCCAAAGGG 
AAAGGACAGT CCCAAACCAG AGTGAATATT AATGCTGCCT TGGTTGAGGA TATAATCAAC 
TTGGAAGAGG TGAATGAAGA AATGAAGTCT GTGATAGAAG CTCTCAAGGA TAATTTCAAT 
AAGACTCTCA ATATAAGGAC CTCACCAGGA TCCCTTGACA AGATTGCTGT GGTAACTGCT 
GACGGGAAGC TTGCTTTAAA CCAGATTAGC CAGATCTCCA TGAAGTCGCC ACAGCTGATT 
TTGGTGAATA TGGCCAGCTT CCCAGAGTGT ACAGCTGCAG CTATCAAGGC TATAAGAGAA 
AGTGGAATGA ATCTGAACCC AGAAGTGGAA GGGACGCTAA TTCGGGTACC CATTCCCCAA 
GTAACCAGAG AGCACAGAGA AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC 
AAAGACTCTT TACGGAAGGT TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT 
ACAGTCTCAG AGGACACCAT TAGGCTAATA GAGAAACAGA TCAGCCAAAT GGCCGATGAC 
ACAGTGGCAG AACTGGACAG GCATCTGGCA GTGAAGACCA AAGAACTCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333272.1
CDS	408..1064
Translation	MAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346343.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346343.1

ORF Insert Sequence:

ATGGCCTATT CAGCTGTACC AGTCCGCCAT TTTGCTACCA AGAAAGCCAA AGCCAAAGGG 
AAAGGACAGT CCCAAACCAG AGTGAATATT AATGCTGCCT TGGTTGAGGA TATAATCAAC 
TTGGAAGAGG TGAATGAAGA AATGAAGTCT GTGATAGAAG CTCTCAAGGA TAATTTCAAT 
AAGACTCTCA ATATAAGGAC CTCACCAGGA TCCCTTGACA AGATTGCTGT GGTAACTGCT 
GACGGGAAGC TTGCTTTAAA CCAGATTAGC CAGATCTCCA TGAAGTCGCC ACAGCTGATT 
TTGGTGAATA TGGCCAGCTT CCCAGAGTGT ACAGCTGCAG CTATCAAGGC TATAAGAGAA 
AGTGGAATGA ATCTGAACCC AGAAGTGGAA GGGACGCTAA TTCGGGTACC CATTCCCCAA 
GTAACCAGAG AGCACAGAGA AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC 
AAAGACTCTT TACGGAAGGT TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT 
ACAGTCTCAG AGGACACCAT TAGGCTAATA GAGAAACAGA TCAGCCAAAT GGCCGATGAC 
ACAGTGGCAG AACTGGACAG GCATCTGGCA GTGAAGACCA AAGAACTCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346343.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333272.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 6, mRNA.

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CloneID	OHu102350
Clone ID Related Accession (Same CDS sequence)	NM_001346343.1 , NM_001346343.2
Accession Version	NM_001346343.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20, BI756457.1, AK300718.1 and AA873020.1. On May 31, 2019 this sequence version replaced NM_001346343.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BI756457.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2153932 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTATT CAGCTGTACC AGTCCGCCAT TTTGCTACCA AGAAAGCCAA AGCCAAAGGG 
AAAGGACAGT CCCAAACCAG AGTGAATATT AATGCTGCCT TGGTTGAGGA TATAATCAAC 
TTGGAAGAGG TGAATGAAGA AATGAAGTCT GTGATAGAAG CTCTCAAGGA TAATTTCAAT 
AAGACTCTCA ATATAAGGAC CTCACCAGGA TCCCTTGACA AGATTGCTGT GGTAACTGCT 
GACGGGAAGC TTGCTTTAAA CCAGATTAGC CAGATCTCCA TGAAGTCGCC ACAGCTGATT 
TTGGTGAATA TGGCCAGCTT CCCAGAGTGT ACAGCTGCAG CTATCAAGGC TATAAGAGAA 
AGTGGAATGA ATCTGAACCC AGAAGTGGAA GGGACGCTAA TTCGGGTACC CATTCCCCAA 
GTAACCAGAG AGCACAGAGA AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC 
AAAGACTCTT TACGGAAGGT TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT 
ACAGTCTCAG AGGACACCAT TAGGCTAATA GAGAAACAGA TCAGCCAAAT GGCCGATGAC 
ACAGTGGCAG AACTGGACAG GCATCTGGCA GTGAAGACCA AAGAACTCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333272.1
CDS	129..785
Translation	MAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346343.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346343.2

ORF Insert Sequence:

ATGGCCTATT CAGCTGTACC AGTCCGCCAT TTTGCTACCA AGAAAGCCAA AGCCAAAGGG 
AAAGGACAGT CCCAAACCAG AGTGAATATT AATGCTGCCT TGGTTGAGGA TATAATCAAC 
TTGGAAGAGG TGAATGAAGA AATGAAGTCT GTGATAGAAG CTCTCAAGGA TAATTTCAAT 
AAGACTCTCA ATATAAGGAC CTCACCAGGA TCCCTTGACA AGATTGCTGT GGTAACTGCT 
GACGGGAAGC TTGCTTTAAA CCAGATTAGC CAGATCTCCA TGAAGTCGCC ACAGCTGATT 
TTGGTGAATA TGGCCAGCTT CCCAGAGTGT ACAGCTGCAG CTATCAAGGC TATAAGAGAA 
AGTGGAATGA ATCTGAACCC AGAAGTGGAA GGGACGCTAA TTCGGGTACC CATTCCCCAA 
GTAACCAGAG AGCACAGAGA AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC 
AAAGACTCTT TACGGAAGGT TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT 
ACAGTCTCAG AGGACACCAT TAGGCTAATA GAGAAACAGA TCAGCCAAAT GGCCGATGAC 
ACAGTGGCAG AACTGGACAG GCATCTGGCA GTGAAGACCA AAGAACTCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346339.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333268.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 4, mRNA.

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CloneID	OHu43581
Clone ID Related Accession (Same CDS sequence)	NM_001346339.1 , XM_011519183.2 , NM_001346339.2
Accession Version	NM_001346339.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On Oct 7, 2016 this sequence version replaced XM_005252302.3. Transcript Variant: This variant (4) encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.94617.1, SRR1803617.189853.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAAGTAAC CAGAGAGCAC AGAGAAATGC TGGTGAAACT GGCCAAACAG 
AACACCAACA AGGCCAAAGA CTCTTTACGG AAGGTTCGCA CCAACTCAAT GAACAAGCTG 
AAGAAATCCA AGGATACAGT CTCAGAGGAC ACCATTAGGC TAATAGAGAA ACAGATCAGC 
CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC TGGCAGTGAA GACCAAAGAA 
CTCCTTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333268.1
CDS	621..1472
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346339.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346339.1

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAAGTAAC CAGAGAGCAC AGAGAAATGC TGGTGAAACT GGCCAAACAG 
AACACCAACA AGGCCAAAGA CTCTTTACGG AAGGTTCGCA CCAACTCAAT GAACAAGCTG 
AAGAAATCCA AGGATACAGT CTCAGAGGAC ACCATTAGGC TAATAGAGAA ACAGATCAGC 
CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC TGGCAGTGAA GACCAAAGAA 
CTCCTTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346339.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333268.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu43581
Clone ID Related Accession (Same CDS sequence)	NM_001346339.1 , XM_011519183.2 , NM_001346339.2
Accession Version	NM_001346339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On May 31, 2019 this sequence version replaced NM_001346339.1. Transcript Variant: This variant (4) encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.248540.1, SRR1803616.94617.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAAGTAAC CAGAGAGCAC AGAGAAATGC TGGTGAAACT GGCCAAACAG 
AACACCAACA AGGCCAAAGA CTCTTTACGG AAGGTTCGCA CCAACTCAAT GAACAAGCTG 
AAGAAATCCA AGGATACAGT CTCAGAGGAC ACCATTAGGC TAATAGAGAA ACAGATCAGC 
CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC TGGCAGTGAA GACCAAAGAA 
CTCCTTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333268.1
CDS	342..1193
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	NM_001346339.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346339.2

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAAGTAAC CAGAGAGCAC AGAGAAATGC TGGTGAAACT GGCCAAACAG 
AACACCAACA AGGCCAAAGA CTCTTTACGG AAGGTTCGCA CCAACTCAAT GAACAAGCTG 
AAGAAATCCA AGGATACAGT CTCAGAGGAC ACCATTAGGC TAATAGAGAA ACAGATCAGC 
CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC TGGCAGTGAA GACCAAAGAA 
CTCCTTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_011519183.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517485.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu43581
Clone ID Related Accession (Same CDS sequence)	NM_001346339.1 , XM_011519183.2 , NM_001346339.2
Accession Version	XM_011519183.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAAGTAAC CAGAGAGCAC AGAGAAATGC TGGTGAAACT GGCCAAACAG 
AACACCAACA AGGCCAAAGA CTCTTTACGG AAGGTTCGCA CCAACTCAAT GAACAAGCTG 
AAGAAATCCA AGGATACAGT CTCAGAGGAC ACCATTAGGC TAATAGAGAA ACAGATCAGC 
CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC TGGCAGTGAA GACCAAAGAA 
CTCCTTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517485.1
CDS	625..1476
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG

Target ORF information:

RefSeq Version	XM_011519183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519183.2

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAAGTAAC CAGAGAGCAC AGAGAAATGC TGGTGAAACT GGCCAAACAG 
AACACCAACA AGGCCAAAGA CTCTTTACGG AAGGTTCGCA CCAACTCAAT GAACAAGCTG 
AAGAAATCCA AGGATACAGT CTCAGAGGAC ACCATTAGGC TAATAGAGAA ACAGATCAGC 
CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC TGGCAGTGAA GACCAAAGAA 
CTCCTTGGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_011519185.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517487.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu74478
Clone ID Related Accession (Same CDS sequence)	XM_011519185.2 , XM_011519185.3
Accession Version	XM_011519185.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011519185.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAATCAGC CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC 
TGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517487.1
CDS	370..1038
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ

Target ORF information:

RefSeq Version	XM_011519185.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519185.2

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAATCAGC CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC 
TGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_011519185.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011517487.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu74478
Clone ID Related Accession (Same CDS sequence)	XM_011519185.2 , XM_011519185.3
Accession Version	XM_011519185.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011519185.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAATCAGC CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC 
TGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011517487.1
CDS	312..980
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPECTAAAIKAIRESGMNLNPEVEGTLIRVPIPQSAKWPMTQWQNWTGIWQ

Target ORF information:

RefSeq Version	XM_011519185.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011519185.3

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGTGTACAGC TGCAGCTATC 
AAGGCTATAA GAGAAAGTGG AATGAATCTG AACCCAGAAG TGGAAGGGAC GCTAATTCGG 
GTACCCATTC CCCAATCAGC CAAATGGCCG ATGACACAGT GGCAGAACTG GACAGGCATC 
TGGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346345.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333274.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu102262
Clone ID Related Accession (Same CDS sequence)	NM_001346345.1 , NM_001346345.2
Accession Version	NM_001346345.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On Oct 7, 2016 this sequence version replaced XM_017015287.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.411749.1, SRR1803611.230696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGA GTAACCAGAG AGCACAGAGA 
AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC AAAGACTCTT TACGGAAGGT 
TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT ACAGTCTCAG AGGACACCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333274.1
CDS	365..967
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPESNQRAQRNAGETGQTEHQQGQRLFTEGSHQLNEQAEEIQGYSLRGHH

Target ORF information:

RefSeq Version	NM_001346345.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346345.1

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGA GTAACCAGAG AGCACAGAGA 
AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC AAAGACTCTT TACGGAAGGT 
TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT ACAGTCTCAG AGGACACCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( NM_001346345.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333274.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu102262
Clone ID Related Accession (Same CDS sequence)	NM_001346345.1 , NM_001346345.2
Accession Version	NM_001346345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162424.20 and AA873020.1. On May 31, 2019 this sequence version replaced NM_001346345.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.411749.1, SRR1803611.230696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGA GTAACCAGAG AGCACAGAGA 
AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC AAAGACTCTT TACGGAAGGT 
TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT ACAGTCTCAG AGGACACCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333274.1
CDS	86..688
Translation	MALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPESNQRAQRNAGETGQTEHQQGQRLFTEGSHQLNEQAEEIQGYSLRGHH

Target ORF information:

RefSeq Version	NM_001346345.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346345.2

ORF Insert Sequence:

ATGGCCTTGG GATTAAAGTG CTTCCGCATG GTCCACCCTA CCTTTCGCAA TTATCTTGCA 
GCCTCTATCA GACCCGTTTC AGAAGTTACA CTGAAGACAG TGCATGAAAG ACAACATGGC 
CATAGGCAAT ACATGGCCTA TTCAGCTGTA CCAGTCCGCC ATTTTGCTAC CAAGAAAGCC 
AAAGCCAAAG GGAAAGGACA GTCCCAAACC AGAGTGAATA TTAATGCTGC CTTGGTTGAG 
GATATAATCA ACTTGGAAGA GGTGAATGAA GAAATGAAGT CTGTGATAGA AGCTCTCAAG 
GATAATTTCA ATAAGACTCT CAATATAAGG ACCTCACCAG GATCCCTTGA CAAGATTGCT 
GTGGTAACTG CTGACGGGAA GCTTGCTTTA AACCAGATTA GCCAGATCTC CATGAAGTCG 
CCACAGCTGA TTTTGGTGAA TATGGCCAGC TTCCCAGAGA GTAACCAGAG AGCACAGAGA 
AATGCTGGTG AAACTGGCCA AACAGAACAC CAACAAGGCC AAAGACTCTT TACGGAAGGT 
TCGCACCAAC TCAATGAACA AGCTGAAGAA ATCCAAGGAT ACAGTCTCAG AGGACACCAT 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_017015286.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870775.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu98998
Clone ID Related Accession (Same CDS sequence)	XM_017015286.1 , XM_017015286.2
Accession Version	XM_017015286.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGAGTAACCA GAGAGCACAG 
AGAAATGCTG GTGAAACTGG CCAAACAGAA CACCAACAAG GCCAAAGACT CTTTACGGAA 
GGTTCGCACC AACTCAATGA ACAAGCTGAA GAAATCCAAG GATACAGTCT CAGAGGACAC 
CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870775.1
CDS	370..1035
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPESNQRAQRNAGETGQTEHQQGQRLFTEGSHQLNEQAEEIQGYSLRGHH

Target ORF information:

RefSeq Version	XM_017015286.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015286.1

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGAGTAACCA GAGAGCACAG 
AGAAATGCTG GTGAAACTGG CCAAACAGAA CACCAACAAG GCCAAAGACT CTTTACGGAA 
GGTTCGCACC AACTCAATGA ACAAGCTGAA GAAATCCAAG GATACAGTCT CAGAGGACAC 
CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRRF ( XM_017015286.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016870775.1 Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu98998
Clone ID Related Accession (Same CDS sequence)	XM_017015286.1 , XM_017015286.2
Accession Version	XM_017015286.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ribosome-recycling factor, mitochondrial isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000009.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017015286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGAGTAACCA GAGAGCACAG 
AGAAATGCTG GTGAAACTGG CCAAACAGAA CACCAACAAG GCCAAAGACT CTTTACGGAA 
GGTTCGCACC AACTCAATGA ACAAGCTGAA GAAATCCAAG GATACAGTCT CAGAGGACAC 
CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016870775.1
CDS	312..977
Translation	MNLNGRWQGVISGCFQGLSSVMALGLKCFRMVHPTFRNYLAASIRPVSEVTLKTVHERQHGHRQYMAYSAVPVRHFATKKAKAKGKGQSQTRVNINAALVEDIINLEEVNEEMKSVIEALKDNFNKTLNIRTSPGSLDKIAVVTADGKLALNQISQISMKSPQLILVNMASFPESNQRAQRNAGETGQTEHQQGQRLFTEGSHQLNEQAEEIQGYSLRGHH

Target ORF information:

RefSeq Version	XM_017015286.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosome recycling factor (MRRF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017015286.2

ORF Insert Sequence:

ATGAATCTAA ATGGCAGATG GCAGGGAGTG ATAAGTGGAT GTTTCCAAGG ATTGTCTTCA 
GTCATGGCCT TGGGATTAAA GTGCTTCCGC ATGGTCCACC CTACCTTTCG CAATTATCTT 
GCAGCCTCTA TCAGACCCGT TTCAGAAGTT ACACTGAAGA CAGTGCATGA AAGACAACAT 
GGCCATAGGC AATACATGGC CTATTCAGCT GTACCAGTCC GCCATTTTGC TACCAAGAAA 
GCCAAAGCCA AAGGGAAAGG ACAGTCCCAA ACCAGAGTGA ATATTAATGC TGCCTTGGTT 
GAGGATATAA TCAACTTGGA AGAGGTGAAT GAAGAAATGA AGTCTGTGAT AGAAGCTCTC 
AAGGATAATT TCAATAAGAC TCTCAATATA AGGACCTCAC CAGGATCCCT TGACAAGATT 
GCTGTGGTAA CTGCTGACGG GAAGCTTGCT TTAAACCAGA TTAGCCAGAT CTCCATGAAG 
TCGCCACAGC TGATTTTGGT GAATATGGCC AGCTTCCCAG AGAGTAACCA GAGAGCACAG 
AGAAATGCTG GTGAAACTGG CCAAACAGAA CACCAACAAG GCCAAAGACT CTTTACGGAA 
GGTTCGCACC AACTCAATGA ACAAGCTGAA GAAATCCAAG GATACAGTCT CAGAGGACAC 
CATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

EF-G2mt is an exclusive recycling factor in mammalian mitochondrial protein synthesis.
Molecular cell35(4)502-10(2009 Aug)
Tsuboi M,Morita H,Nozaki Y,Akama K,Ueda T,Ito K,Nierhaus KH,Takeuchi N

The human mitochondrial ribosome recycling factor is essential for cell viability.
Nucleic acids research36(18)5787-99(2008 Oct)
Rorbach J,Richter R,Wessels HJ,Wydro M,Pekalski M,Farhoud M,K?hl I,Gaisne M,Bonnefoy N,Smeitink JA,Lightowlers RN,Chrzanowska-Lightowlers ZM

DNA sequence and analysis of human chromosome 9.
Nature429(6990)369-74(2004 May)
Humphray SJ,Oliver K,Hunt AR,Plumb RW,Loveland JE,Howe KL,Andrews TD,Searle S,Hunt SE,Scott CE,Jones MC,Ainscough R,Almeida JP,Ambrose KD,Ashwell RI,Babbage AK,Babbage S,Bagguley CL,Bailey J,Banerjee R,Barker DJ,Barlow KF,Bates K,Beasley H,Beasley O,Bird CP,Bray-Allen S,Brown AJ,Brown JY,Burford D,Burrill W,Burton J,Carder C,Carter NP,Chapman JC,Chen Y,Clarke G,Clark SY,Clee CM,Clegg S,Collier RE,Corby N,Crosier M,Cummings AT,Davies J,Dhami P,Dunn M,Dutta I,Dyer LW,Earthrowl ME,Faulkner L,Fleming CJ,Frankish A,Frankland JA,French L,Fricker DG,Garner P,Garnett J,Ghori J,Gilbert JG,Glison C,Grafham DV,Gribble S,Griffiths C,Griffiths-Jones S,Grocock R,Guy J,Hall RE,Hammond S,Harley JL,Harrison ES,Hart EA,Heath PD,Henderson CD,Hopkins BL,Howard PJ,Howden PJ,Huckle E,Johnson C,Johnson D,Joy AA,Kay M,Keenan S,Kershaw JK,Kimberley AM,King A,Knights A,Laird GK,Langford C,Lawlor S,Leongamornlert DA,Leversha M,Lloyd C,Lloyd DM,Lovell J,Martin S,Mashreghi-Mohammadi M,Matthews L,McLaren S,McLay KE,McMurray A,Milne S,Nickerson T,Nisbett J,Nordsiek G,Pearce AV,Peck AI,Porter KM,Pandian R,Pelan S,Phillimore B,Povey S,Ramsey Y,Rand V,Scharfe M,Sehra HK,Shownkeen R,Sims SK,Skuce CD,Smith M,Steward CA,Swarbreck D,Sycamore N,Tester J,Thorpe A,Tracey A,Tromans A,Thomas DW,Wall M,Wallis JM,West AP,Whitehead SL,Willey DL,Williams SA,Wilming L,Wray PW,Young L,Ashurst JL,Coulson A,Bl?cker H,Durbin R,Sulston JE,Hubbard T,Jackson MJ,Bentley DR,Beck S,Rogers J,Dunham I

Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping.
Cytogenetics and cell genetics88(1-2)91-2(2000)
Hansen LL,J?rgensen R,Justesen J

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MRRF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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