ZNF300 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF300
Entrez Gene ID	91975
Full Name	zinc finger protein 300
General protein information	Preferred Names zinc finger protein 300 Names zinc finger protein 300 kruppel-like zinc finger protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q33.1
Summary	The protein encoded by this gene is a C2H2-type zinc finger DNA binding protein and likely transcriptional regulator. The function of this protein is not yet known. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010].
Disorder MIM:	612429

mRNA and Protein(s)

mRNA	Protein	Name
NM_052860.2	NP_443092.1	zinc finger protein 300 isoform 2
XM_011537702.2	XP_011536004.1	zinc finger protein 300 isoform X1
NM_001172831.1	NP_001166302.1	zinc finger protein 300 isoform 1
XM_006714806.1	XP_006714869.1	zinc finger protein 300 isoform X1
NM_001172832.1	NP_001166303.1	zinc finger protein 300 isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	ZNF300	NP_001166302.1
Bos taurus (cattle)	ZNF300	XP_002689355.2
Pan troglodytes (chimpanzee)	ZNF300	XP_003310961.2
	ZNF300	XP_001109278.1
Canis lupus familiaris (dog)	ZNF300	XP_005619334.1

Gene Ontology
Bibliography

Related articles in PubMed

ZNF300P1 encodes a lincRNA that regulates cell polarity and is epigenetically silenced in type II epithelial ovarian cancer.
Gloss B, Moran-Jones K, Lin V, Gonzalez M, Scurry J, Hacker NF, Sutherland RL, Clark SJ, Samimi G
Molecular cancer133(2014 Jan)

Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1.
Wang W, Cai J, Wu Y, Hu L, Chen Z, Hu J, Chen Z, Li W, Guo M, Huang Z
Cellular and molecular life sciences : CMLS70(20)3947-58(2013 Oct)

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Jostins L, Ripke S, Weersma RK, Duerr RH, McGovern DP, Hui KY, Lee JC, Schumm LP, Sharma Y, Anderson CA, Essers J, Mitrovic M, Ning K, Cleynen I, Theatre E, Spain SL, Raychaudhuri S, Goyette P, Wei Z, Abraham C, Achkar JP, Ahmad T, Amininejad L, Ananthakrishnan AN, Andersen V, Andrews JM, Baidoo L, Balschun T, Bampton PA, Bitton A, Boucher G, Brand S, B?ning C, Cohain A, Cichon S, D'Amato M, De Jong D, Devaney KL, Dubinsky M, Edwards C, Ellinghaus D, Ferguson LR, Franchimont D, Fransen K, Gearry R, Georges M, Gieger C, Glas J, Haritunians T, Hart A, Hawkey C, Hedl M, Hu X, Karlsen TH, Kupcinskas L, Kugathasan S, Latiano A, Laukens D, Lawrance IC, Lees CW, Louis E, Mahy G, Mansfield J, Morgan AR, Mowat C, Newman W, Palmieri O, Ponsioen CY, Potocnik U, Prescott NJ, Regueiro M, Rotter JI, Russell RK, Sanderson JD, Sans M, Satsangi J, Schreiber S, Simms LA, Sventoraityte J, Targan SR, Taylor KD, Tremelling M, Verspaget HW, De Vos M, Wijmenga C, Wilson DC, Winkelmann J, Xavier RJ, Zeissig S, Zhang B, Zhang CK, Zhao H, , Silverberg MS, Annese V, Hakonarson H, Brant SR, Radford-Smith G, Mathew CG, Rioux JD, Schadt EE, Daly MJ, Franke A, Parkes M, Vermeire S, Barrett JC, Cho JH
Nature491(7422)119-24(2012 Nov)

Overexpression of the human ZNF300 gene enhances growth and metastasis of cancer cells through activating NF-kB pathway.
Wang T, Wang XG, Xu JH, Wu XP, Qiu HL, Yi H, Li WX
Journal of cellular and molecular medicine16(5)1134-45(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings further support ZNF300P1 as frequently methylated in ovarian cancer and reveal a novel function for ZNF300P1 lincRNA expression
Title: ZNF300P1 encodes a lincRNA that regulates cell polarity and is epigenetically silenced in type II epithelial ovarian cancer.

A novel function for ZNF300 in tumour development was identified that may uniquely link inflammation and NF-kappa B to tumourigenesis in humans but not in mice.
Title: Overexpression of the human ZNF300 gene enhances growth and metastasis of cancer cells through activating NF-kB pathway.

Data show that co-expression of ZNF300 and Egr1 lead to enhanced IL-2Rbeta promoter activity, and suggest that ZNF300 is another regulator of the human IL-2Rbeta promoter.
Title: ZNF300, a recently identified human transcription factor, activates the human IL-2R? promoter through the overlapping ZNF300/EGR1 binding site.

ZNF300 was activated by PU.1 and suggested that the regulation may be involved in the progression of leukemia development and hematopoietic differentiation.
Title: PU.1 can regulate the ZNF300 promoter in APL-derived promyelocytes HL-60.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Genome-wide searching of rare genetic variants in WTCCC data.

The following ZNF300 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF300 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07676	NM_001172832.1 Latest version!	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu08694	NM_052860.2 Latest version!	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21418	NM_001172831.1 Latest version!	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$259.50-$363.30 $519.00
OHu08694	XM_006714806.1 Latest version!	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu39914	XM_011537702.2 Latest version!	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF300 ( NM_001172832.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166303.1 Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07676
Clone ID Related Accession (Same CDS sequence)	NM_001172832.1
Accession Version	NM_001172832.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	zinc finger protein 300 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA497503.1, DB094933.1, AF395541.1, AC010441.6 and BM824443.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.254480.1, SRR1660807.191057.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGGAGA ACTACAGCCA CCTGGTCTCA ATGGGGTATC CAGTTTCCAA ACCAGATGTC 
ATCTCCAAGT TGGAACAAGG AGAAGAGCCA TGGATCATAA AGGGAGACAT ATCAAATTGG 
ATCTATCCAG ATGAATATCA GGCAGATGGG AGACAAGACA GGAAGAGTAA CCTTCACAAC 
TCCCAGTCAT GTATTTTGGG GACAGTTTCC TTCCATCATA AGATACTGAA AGGAGTCACA 
AGGGATGGTT CATTGTGCTC CATTTTAAAA GTCTGTCAAG GTGATGGTCA GCTGCAGAGA 
TTTCTAGAGA ATCAAGACAA ACTCTTCAGG CAGGTCACAT TTGTTAACAG CAAAACAGTG 
ACTGAGGCAT CAGGGCATAA ATATAATCCA CTGGGGAAAA TATTTCAAGA GTGCATAGAA 
ACAGATATAT CAATACAGAG ATTCCATAAA TATGATGCTT TTAAAAAGAA CTTAAAACCA 
AATATTGACC TACCGAGTTG TTATAAGAGC AATTCAAGAA AAAAACCTGA TCAGAGTTTT 
GGAGGTGGAA AATCATCTAG CCAGAGTGAG CCCAATTCTA ATCTTGAGAA GATTCACAAT 
GGAGTAATAC CTTTTGATGA TAATCAGTGT GGAAACGTTT TTAGAAATAC ACAATCCCTT 
ATTCAATATC AGAATGTGGA AACTAAAGAG AAAAGCTGTG TATGTGTTAC ATGTGGAAAA 
GCCTTTGCTA AGAAGTCACA ACTCATTGTA CATCAAAGAA TTCATACTGG AAAGAAACCA 
TATGATTGTG GTGCATGCGG AAAAGCCTTC AGTGAGAAGT TTCATCTTGT TGTACATCAG 
AGAACTCATA CTGGGGAGAA ACCTTATGAT TGTTCTGAAT GTGGAAAAGC CTTCTCTCAG 
AAATCGTCCC TTATTATACA TCAGAGAGTT CACACTGGGG AAAAACCCTA TGAATGTAGT 
GAATGCGGGA AAGCCTTCTC CCAGAAATCA CCCCTCATTA TACATCAGAG AATACATACT 
GGGGAAAAAC CCTATGAATG TAGAGAGTGT GGGAAGGCCT TTTCCCAGAA GTCACAGCTG 
ATTATACACC ACAGAGCTCA TACTGGAGAG AAGCCGTATG AGTGTACCGA ATGTGGGAAA 
GCCTTCTGTG AGAAGTCCCA CCTCATTATA CATAAAAGAA TTCACACTGG TGAGAAACCC 
TACAAATGTG CTCAATGTGA GGAAGCCTTC AGCAGGAAGA CAGAACTCAT TACACATCAG 
TTAGTTCATA CTGGGGAAAA ACCTTATGAA TGTACTGAAT GTGGAAAGAC ATTCTCCCGC 
AAGTCACAGC TCATCATACA TCAGAGAACA CATACTGGAG AAAAACCCTA TAAATGTAGT 
GAATGTGGCA AAGCCTTCTG CCAGAAGTCA CATCTCATTG GACATCAGAG AATTCACACA 
GGAGAAAAAC CTTATATATG TACTGAATGT GGGAAAGCCT TCTCTCAGAA GTCCCACCTT 
CCGGGACACC AGCGAATTCA TACAGGAGAG AAACCTTACA TATGTGCTGA ATGTGGAAAG 
GCCTTTTCTC AGAAGTCAGA CCTTGTTTTA CATCAGAGGA TTCATACTGG GGAAAGACCC 
TATCAATGTG CTATATGTGG GAAGGCCTTC ATCCAGAAGT CACAACTAAC TGTACACCAG 
AGAATTCACA CAGTGGTAAA ATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166303.1
CDS	488..2194
Translation	MLENYSHLVSMGYPVSKPDVISKLEQGEEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSILKVCQGDGQLQRFLENQDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNLKPNIDLPSCYKSNSRKKPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVETKEKSCVCVTCGKAFAKKSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAFSQKSSLIIHQRVHTGEKPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHTGEKPYECTECGKAFCEKSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTFSRKSQLIIHQRTHTGEKPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHTGEKPYICAECGKAFSQKSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS

Target ORF information:

RefSeq Version	NM_001172832.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172832.1

ORF Insert Sequence:

ATGCTGGAGA ACTACAGCCA CCTGGTCTCA ATGGGGTATC CAGTTTCCAA ACCAGATGTC 
ATCTCCAAGT TGGAACAAGG AGAAGAGCCA TGGATCATAA AGGGAGACAT ATCAAATTGG 
ATCTATCCAG ATGAATATCA GGCAGATGGG AGACAAGACA GGAAGAGTAA CCTTCACAAC 
TCCCAGTCAT GTATTTTGGG GACAGTTTCC TTCCATCATA AGATACTGAA AGGAGTCACA 
AGGGATGGTT CATTGTGCTC CATTTTAAAA GTCTGTCAAG GTGATGGTCA GCTGCAGAGA 
TTTCTAGAGA ATCAAGACAA ACTCTTCAGG CAGGTCACAT TTGTTAACAG CAAAACAGTG 
ACTGAGGCAT CAGGGCATAA ATATAATCCA CTGGGGAAAA TATTTCAAGA GTGCATAGAA 
ACAGATATAT CAATACAGAG ATTCCATAAA TATGATGCTT TTAAAAAGAA CTTAAAACCA 
AATATTGACC TACCGAGTTG TTATAAGAGC AATTCAAGAA AAAAACCTGA TCAGAGTTTT 
GGAGGTGGAA AATCATCTAG CCAGAGTGAG CCCAATTCTA ATCTTGAGAA GATTCACAAT 
GGAGTAATAC CTTTTGATGA TAATCAGTGT GGAAACGTTT TTAGAAATAC ACAATCCCTT 
ATTCAATATC AGAATGTGGA AACTAAAGAG AAAAGCTGTG TATGTGTTAC ATGTGGAAAA 
GCCTTTGCTA AGAAGTCACA ACTCATTGTA CATCAAAGAA TTCATACTGG AAAGAAACCA 
TATGATTGTG GTGCATGCGG AAAAGCCTTC AGTGAGAAGT TTCATCTTGT TGTACATCAG 
AGAACTCATA CTGGGGAGAA ACCTTATGAT TGTTCTGAAT GTGGAAAAGC CTTCTCTCAG 
AAATCGTCCC TTATTATACA TCAGAGAGTT CACACTGGGG AAAAACCCTA TGAATGTAGT 
GAATGCGGGA AAGCCTTCTC CCAGAAATCA CCCCTCATTA TACATCAGAG AATACATACT 
GGGGAAAAAC CCTATGAATG TAGAGAGTGT GGGAAGGCCT TTTCCCAGAA GTCACAGCTG 
ATTATACACC ACAGAGCTCA TACTGGAGAG AAGCCGTATG AGTGTACCGA ATGTGGGAAA 
GCCTTCTGTG AGAAGTCCCA CCTCATTATA CATAAAAGAA TTCACACTGG TGAGAAACCC 
TACAAATGTG CTCAATGTGA GGAAGCCTTC AGCAGGAAGA CAGAACTCAT TACACATCAG 
TTAGTTCATA CTGGGGAAAA ACCTTATGAA TGTACTGAAT GTGGAAAGAC ATTCTCCCGC 
AAGTCACAGC TCATCATACA TCAGAGAACA CATACTGGAG AAAAACCCTA TAAATGTAGT 
GAATGTGGCA AAGCCTTCTG CCAGAAGTCA CATCTCATTG GACATCAGAG AATTCACACA 
GGAGAAAAAC CTTATATATG TACTGAATGT GGGAAAGCCT TCTCTCAGAA GTCCCACCTT 
CCGGGACACC AGCGAATTCA TACAGGAGAG AAACCTTACA TATGTGCTGA ATGTGGAAAG 
GCCTTTTCTC AGAAGTCAGA CCTTGTTTTA CATCAGAGGA TTCATACTGG GGAAAGACCC 
TATCAATGTG CTATATGTGG GAAGGCCTTC ATCCAGAAGT CACAACTAAC TGTACACCAG 
AGAATTCACA CAGTGGTAAA ATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF300 ( NM_052860.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_443092.1 Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08694
Clone ID Related Accession (Same CDS sequence)	NM_052860.2 , XM_006714806.1
Accession Version	NM_052860.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	zinc finger protein 300 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA497503.1, AF395541.1, AC010441.6 and BM824443.1. On Mar 4, 2010 this sequence version replaced NM_052860.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.155602.1, AK096465.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATGAAGT CCCAGGGGTT AGTATCATTC AAGGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AACTTGACCC TTCTCAGAGG ACCCTGTACA GGGATGTGAT GCTGGAGAAC 
TACAGCCACC TGGTCTCAAT GGGGTATCCA GTTTCCAAAC CAGATGTCAT CTCCAAGTTG 
GAACAAGGAG AAGAGCCATG GATCATAAAG GGAGACATAT CAAATTGGAT CTATCCAGAT 
GAATATCAGG CAGATGGGAG ACAAGACAGG AAGAGTAACC TTCACAACTC CCAGTCATGT 
ATTTTGGGGA CAGTTTCCTT CCATCATAAG ATACTGAAAG GAGTCACAAG GGATGGTTCA 
TTGTGCTCCA TTTTAAAAGT CTGTCAAGGT GATGGTCAGC TGCAGAGATT TCTAGAGAAT 
CAAGACAAAC TCTTCAGGCA GGTCACATTT GTTAACAGCA AAACAGTGAC TGAGGCATCA 
GGGCATAAAT ATAATCCACT GGGGAAAATA TTTCAAGAGT GCATAGAAAC AGATATATCA 
ATACAGAGAT TCCATAAATA TGATGCTTTT AAAAAGAACT TAAAACCAAA TATTGACCTA 
CCGAGTTGTT ATAAGAGCAA TTCAAGAAAA AAACCTGATC AGAGTTTTGG AGGTGGAAAA 
TCATCTAGCC AGAGTGAGCC CAATTCTAAT CTTGAGAAGA TTCACAATGG AGTAATACCT 
TTTGATGATA ATCAGTGTGG AAACGTTTTT AGAAATACAC AATCCCTTAT TCAATATCAG 
AATGTGGAAA CTAAAGAGAA AAGCTGTGTA TGTGTTACAT GTGGAAAAGC CTTTGCTAAG 
AAGTCACAAC TCATTGTACA TCAAAGAATT CATACTGGAA AGAAACCATA TGATTGTGGT 
GCATGCGGAA AAGCCTTCAG TGAGAAGTTT CATCTTGTTG TACATCAGAG AACTCATACT 
GGGGAGAAAC CTTATGATTG TTCTGAATGT GGAAAAGCCT TCTCTCAGAA ATCGTCCCTT 
ATTATACATC AGAGAGTTCA CACTGGGGAA AAACCCTATG AATGTAGTGA ATGCGGGAAA 
GCCTTCTCCC AGAAATCACC CCTCATTATA CATCAGAGAA TACATACTGG GGAAAAACCC 
TATGAATGTA GAGAGTGTGG GAAGGCCTTT TCCCAGAAGT CACAGCTGAT TATACACCAC 
AGAGCTCATA CTGGAGAGAA GCCGTATGAG TGTACCGAAT GTGGGAAAGC CTTCTGTGAG 
AAGTCCCACC TCATTATACA TAAAAGAATT CACACTGGTG AGAAACCCTA CAAATGTGCT 
CAATGTGAGG AAGCCTTCAG CAGGAAGACA GAACTCATTA CACATCAGTT AGTTCATACT 
GGGGAAAAAC CTTATGAATG TACTGAATGT GGAAAGACAT TCTCCCGCAA GTCACAGCTC 
ATCATACATC AGAGAACACA TACTGGAGAA AAACCCTATA AATGTAGTGA ATGTGGCAAA 
GCCTTCTGCC AGAAGTCACA TCTCATTGGA CATCAGAGAA TTCACACAGG AGAAAAACCT 
TATATATGTA CTGAATGTGG GAAAGCCTTC TCTCAGAAGT CCCACCTTCC GGGACACCAG 
CGAATTCATA CAGGAGAGAA ACCTTACATA TGTGCTGAAT GTGGAAAGGC CTTTTCTCAG 
AAGTCAGACC TTGTTTTACA TCAGAGGATT CATACTGGGG AAAGACCCTA TCAATGTGCT 
ATATGTGGGA AGGCCTTCAT CCAGAAGTCA CAACTAACTG TACACCAGAG AATTCACACA 
GTGGTAAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_443092.1
CDS	422..2236
Translation	MMKSQGLVSFKDVAVDFTQEEWQQLDPSQRTLYRDVMLENYSHLVSMGYPVSKPDVISKLEQGEEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSILKVCQGDGQLQRFLENQDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNLKPNIDLPSCYKSNSRKKPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVETKEKSCVCVTCGKAFAKKSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAFSQKSSLIIHQRVHTGEKPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHTGEKPYECTECGKAFCEKSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTFSRKSQLIIHQRTHTGEKPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHTGEKPYICAECGKAFSQKSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS

Target ORF information:

RefSeq Version	NM_052860.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052860.2

ORF Insert Sequence:

ATGATGAAGT CCCAGGGGTT AGTATCATTC AAGGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AACTTGACCC TTCTCAGAGG ACCCTGTACA GGGATGTGAT GCTGGAGAAC 
TACAGCCACC TGGTCTCAAT GGGGTATCCA GTTTCCAAAC CAGATGTCAT CTCCAAGTTG 
GAACAAGGAG AAGAGCCATG GATCATAAAG GGAGACATAT CAAATTGGAT CTATCCAGAT 
GAATATCAGG CAGATGGGAG ACAAGACAGG AAGAGTAACC TTCACAACTC CCAGTCATGT 
ATTTTGGGGA CAGTTTCCTT CCATCATAAG ATACTGAAAG GAGTCACAAG GGATGGTTCA 
TTGTGCTCCA TTTTAAAAGT CTGTCAAGGT GATGGTCAGC TGCAGAGATT TCTAGAGAAT 
CAAGACAAAC TCTTCAGGCA GGTCACATTT GTTAACAGCA AAACAGTGAC TGAGGCATCA 
GGGCATAAAT ATAATCCACT GGGGAAAATA TTTCAAGAGT GCATAGAAAC AGATATATCA 
ATACAGAGAT TCCATAAATA TGATGCTTTT AAAAAGAACT TAAAACCAAA TATTGACCTA 
CCGAGTTGTT ATAAGAGCAA TTCAAGAAAA AAACCTGATC AGAGTTTTGG AGGTGGAAAA 
TCATCTAGCC AGAGTGAGCC CAATTCTAAT CTTGAGAAGA TTCACAATGG AGTAATACCT 
TTTGATGATA ATCAGTGTGG AAACGTTTTT AGAAATACAC AATCCCTTAT TCAATATCAG 
AATGTGGAAA CTAAAGAGAA AAGCTGTGTA TGTGTTACAT GTGGAAAAGC CTTTGCTAAG 
AAGTCACAAC TCATTGTACA TCAAAGAATT CATACTGGAA AGAAACCATA TGATTGTGGT 
GCATGCGGAA AAGCCTTCAG TGAGAAGTTT CATCTTGTTG TACATCAGAG AACTCATACT 
GGGGAGAAAC CTTATGATTG TTCTGAATGT GGAAAAGCCT TCTCTCAGAA ATCGTCCCTT 
ATTATACATC AGAGAGTTCA CACTGGGGAA AAACCCTATG AATGTAGTGA ATGCGGGAAA 
GCCTTCTCCC AGAAATCACC CCTCATTATA CATCAGAGAA TACATACTGG GGAAAAACCC 
TATGAATGTA GAGAGTGTGG GAAGGCCTTT TCCCAGAAGT CACAGCTGAT TATACACCAC 
AGAGCTCATA CTGGAGAGAA GCCGTATGAG TGTACCGAAT GTGGGAAAGC CTTCTGTGAG 
AAGTCCCACC TCATTATACA TAAAAGAATT CACACTGGTG AGAAACCCTA CAAATGTGCT 
CAATGTGAGG AAGCCTTCAG CAGGAAGACA GAACTCATTA CACATCAGTT AGTTCATACT 
GGGGAAAAAC CTTATGAATG TACTGAATGT GGAAAGACAT TCTCCCGCAA GTCACAGCTC 
ATCATACATC AGAGAACACA TACTGGAGAA AAACCCTATA AATGTAGTGA ATGTGGCAAA 
GCCTTCTGCC AGAAGTCACA TCTCATTGGA CATCAGAGAA TTCACACAGG AGAAAAACCT 
TATATATGTA CTGAATGTGG GAAAGCCTTC TCTCAGAAGT CCCACCTTCC GGGACACCAG 
CGAATTCATA CAGGAGAGAA ACCTTACATA TGTGCTGAAT GTGGAAAGGC CTTTTCTCAG 
AAGTCAGACC TTGTTTTACA TCAGAGGATT CATACTGGGG AAAGACCCTA TCAATGTGCT 
ATATGTGGGA AGGCCTTCAT CCAGAAGTCA CAACTAACTG TACACCAGAG AATTCACACA 
GTGGTAAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF300 ( NM_001172831.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166302.1 Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21418
Clone ID Related Accession (Same CDS sequence)	NM_001172831.1
Accession Version	NM_001172831.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	zinc finger protein 300 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA497503.1, DC376791.1, AF395541.1, AC010441.6 and BM824443.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.130074.1, AK300528.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2161674, SAMEA2467146 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGCCTG CTGAGACTTC TTCATCTGGC CTTTTGGAAG AGCAAAAAAT GATGAAGTCC 
CAGGGGTTAG TATCATTCAA GGATGTGGCT GTGGATTTCA CCCAGGAGGA GTGGCAGCAA 
CTTGACCCTT CTCAGAGGAC CCTGTACAGG GATGTGATGC TGGAGAACTA CAGCCACCTG 
GTCTCAATGG GGTATCCAGT TTCCAAACCA GATGTCATCT CCAAGTTGGA ACAAGGAGAA 
GAGCCATGGA TCATAAAGGG AGACATATCA AATTGGATCT ATCCAGATGA ATATCAGGCA 
GATGGGAGAC AAGACAGGAA GAGTAACCTT CACAACTCCC AGTCATGTAT TTTGGGGACA 
GTTTCCTTCC ATCATAAGAT ACTGAAAGGA GTCACAAGGG ATGGTTCATT GTGCTCCATT 
TTAAAAGTCT GTCAAGGTGA TGGTCAGCTG CAGAGATTTC TAGAGAATCA AGACAAACTC 
TTCAGGCAGG TCACATTTGT TAACAGCAAA ACAGTGACTG AGGCATCAGG GCATAAATAT 
AATCCACTGG GGAAAATATT TCAAGAGTGC ATAGAAACAG ATATATCAAT ACAGAGATTC 
CATAAATATG ATGCTTTTAA AAAGAACTTA AAACCAAATA TTGACCTACC GAGTTGTTAT 
AAGAGCAATT CAAGAAAAAA ACCTGATCAG AGTTTTGGAG GTGGAAAATC ATCTAGCCAG 
AGTGAGCCCA ATTCTAATCT TGAGAAGATT CACAATGGAG TAATACCTTT TGATGATAAT 
CAGTGTGGAA ACGTTTTTAG AAATACACAA TCCCTTATTC AATATCAGAA TGTGGAAACT 
AAAGAGAAAA GCTGTGTATG TGTTACATGT GGAAAAGCCT TTGCTAAGAA GTCACAACTC 
ATTGTACATC AAAGAATTCA TACTGGAAAG AAACCATATG ATTGTGGTGC ATGCGGAAAA 
GCCTTCAGTG AGAAGTTTCA TCTTGTTGTA CATCAGAGAA CTCATACTGG GGAGAAACCT 
TATGATTGTT CTGAATGTGG AAAAGCCTTC TCTCAGAAAT CGTCCCTTAT TATACATCAG 
AGAGTTCACA CTGGGGAAAA ACCCTATGAA TGTAGTGAAT GCGGGAAAGC CTTCTCCCAG 
AAATCACCCC TCATTATACA TCAGAGAATA CATACTGGGG AAAAACCCTA TGAATGTAGA 
GAGTGTGGGA AGGCCTTTTC CCAGAAGTCA CAGCTGATTA TACACCACAG AGCTCATACT 
GGAGAGAAGC CGTATGAGTG TACCGAATGT GGGAAAGCCT TCTGTGAGAA GTCCCACCTC 
ATTATACATA AAAGAATTCA CACTGGTGAG AAACCCTACA AATGTGCTCA ATGTGAGGAA 
GCCTTCAGCA GGAAGACAGA ACTCATTACA CATCAGTTAG TTCATACTGG GGAAAAACCT 
TATGAATGTA CTGAATGTGG AAAGACATTC TCCCGCAAGT CACAGCTCAT CATACATCAG 
AGAACACATA CTGGAGAAAA ACCCTATAAA TGTAGTGAAT GTGGCAAAGC CTTCTGCCAG 
AAGTCACATC TCATTGGACA TCAGAGAATT CACACAGGAG AAAAACCTTA TATATGTACT 
GAATGTGGGA AAGCCTTCTC TCAGAAGTCC CACCTTCCGG GACACCAGCG AATTCATACA 
GGAGAGAAAC CTTACATATG TGCTGAATGT GGAAAGGCCT TTTCTCAGAA GTCAGACCTT 
GTTTTACATC AGAGGATTCA TACTGGGGAA AGACCCTATC AATGTGCTAT ATGTGGGAAG 
GCCTTCATCC AGAAGTCACA ACTAACTGTA CACCAGAGAA TTCACACAGT GGTAAAATCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166302.1
CDS	429..2291
Translation	MVPAETSSSGLLEEQKMMKSQGLVSFKDVAVDFTQEEWQQLDPSQRTLYRDVMLENYSHLVSMGYPVSKPDVISKLEQGEEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSILKVCQGDGQLQRFLENQDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNLKPNIDLPSCYKSNSRKKPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVETKEKSCVCVTCGKAFAKKSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAFSQKSSLIIHQRVHTGEKPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHTGEKPYECTECGKAFCEKSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTFSRKSQLIIHQRTHTGEKPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHTGEKPYICAECGKAFSQKSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS

Target ORF information:

RefSeq Version	NM_001172831.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001172831.1

ORF Insert Sequence:

ATGGTGCCTG CTGAGACTTC TTCATCTGGC CTTTTGGAAG AGCAAAAAAT GATGAAGTCC 
CAGGGGTTAG TATCATTCAA GGATGTGGCT GTGGATTTCA CCCAGGAGGA GTGGCAGCAA 
CTTGACCCTT CTCAGAGGAC CCTGTACAGG GATGTGATGC TGGAGAACTA CAGCCACCTG 
GTCTCAATGG GGTATCCAGT TTCCAAACCA GATGTCATCT CCAAGTTGGA ACAAGGAGAA 
GAGCCATGGA TCATAAAGGG AGACATATCA AATTGGATCT ATCCAGATGA ATATCAGGCA 
GATGGGAGAC AAGACAGGAA GAGTAACCTT CACAACTCCC AGTCATGTAT TTTGGGGACA 
GTTTCCTTCC ATCATAAGAT ACTGAAAGGA GTCACAAGGG ATGGTTCATT GTGCTCCATT 
TTAAAAGTCT GTCAAGGTGA TGGTCAGCTG CAGAGATTTC TAGAGAATCA AGACAAACTC 
TTCAGGCAGG TCACATTTGT TAACAGCAAA ACAGTGACTG AGGCATCAGG GCATAAATAT 
AATCCACTGG GGAAAATATT TCAAGAGTGC ATAGAAACAG ATATATCAAT ACAGAGATTC 
CATAAATATG ATGCTTTTAA AAAGAACTTA AAACCAAATA TTGACCTACC GAGTTGTTAT 
AAGAGCAATT CAAGAAAAAA ACCTGATCAG AGTTTTGGAG GTGGAAAATC ATCTAGCCAG 
AGTGAGCCCA ATTCTAATCT TGAGAAGATT CACAATGGAG TAATACCTTT TGATGATAAT 
CAGTGTGGAA ACGTTTTTAG AAATACACAA TCCCTTATTC AATATCAGAA TGTGGAAACT 
AAAGAGAAAA GCTGTGTATG TGTTACATGT GGAAAAGCCT TTGCTAAGAA GTCACAACTC 
ATTGTACATC AAAGAATTCA TACTGGAAAG AAACCATATG ATTGTGGTGC ATGCGGAAAA 
GCCTTCAGTG AGAAGTTTCA TCTTGTTGTA CATCAGAGAA CTCATACTGG GGAGAAACCT 
TATGATTGTT CTGAATGTGG AAAAGCCTTC TCTCAGAAAT CGTCCCTTAT TATACATCAG 
AGAGTTCACA CTGGGGAAAA ACCCTATGAA TGTAGTGAAT GCGGGAAAGC CTTCTCCCAG 
AAATCACCCC TCATTATACA TCAGAGAATA CATACTGGGG AAAAACCCTA TGAATGTAGA 
GAGTGTGGGA AGGCCTTTTC CCAGAAGTCA CAGCTGATTA TACACCACAG AGCTCATACT 
GGAGAGAAGC CGTATGAGTG TACCGAATGT GGGAAAGCCT TCTGTGAGAA GTCCCACCTC 
ATTATACATA AAAGAATTCA CACTGGTGAG AAACCCTACA AATGTGCTCA ATGTGAGGAA 
GCCTTCAGCA GGAAGACAGA ACTCATTACA CATCAGTTAG TTCATACTGG GGAAAAACCT 
TATGAATGTA CTGAATGTGG AAAGACATTC TCCCGCAAGT CACAGCTCAT CATACATCAG 
AGAACACATA CTGGAGAAAA ACCCTATAAA TGTAGTGAAT GTGGCAAAGC CTTCTGCCAG 
AAGTCACATC TCATTGGACA TCAGAGAATT CACACAGGAG AAAAACCTTA TATATGTACT 
GAATGTGGGA AAGCCTTCTC TCAGAAGTCC CACCTTCCGG GACACCAGCG AATTCATACA 
GGAGAGAAAC CTTACATATG TGCTGAATGT GGAAAGGCCT TTTCTCAGAA GTCAGACCTT 
GTTTTACATC AGAGGATTCA TACTGGGGAA AGACCCTATC AATGTGCTAT ATGTGGGAAG 
GCCTTCATCC AGAAGTCACA ACTAACTGTA CACCAGAGAA TTCACACAGT GGTAAAATCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF300 ( XM_006714806.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714869.1 Homo sapiens zinc finger protein 300 (ZNF300), transcript variant X3, mRNA.

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CloneID	OHu08694
Clone ID Related Accession (Same CDS sequence)	NM_052860.2 , XM_006714806.1
Accession Version	XM_006714806.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	zinc finger protein 300 isoform X1
Comment

ATGATGAAGT CCCAGGGGTT AGTATCATTC AAGGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AACTTGACCC TTCTCAGAGG ACCCTGTACA GGGATGTGAT GCTGGAGAAC 
TACAGCCACC TGGTCTCAAT GGGGTATCCA GTTTCCAAAC CAGATGTCAT CTCCAAGTTG 
GAACAAGGAG AAGAGCCATG GATCATAAAG GGAGACATAT CAAATTGGAT CTATCCAGAT 
GAATATCAGG CAGATGGGAG ACAAGACAGG AAGAGTAACC TTCACAACTC CCAGTCATGT 
ATTTTGGGGA CAGTTTCCTT CCATCATAAG ATACTGAAAG GAGTCACAAG GGATGGTTCA 
TTGTGCTCCA TTTTAAAAGT CTGTCAAGGT GATGGTCAGC TGCAGAGATT TCTAGAGAAT 
CAAGACAAAC TCTTCAGGCA GGTCACATTT GTTAACAGCA AAACAGTGAC TGAGGCATCA 
GGGCATAAAT ATAATCCACT GGGGAAAATA TTTCAAGAGT GCATAGAAAC AGATATATCA 
ATACAGAGAT TCCATAAATA TGATGCTTTT AAAAAGAACT TAAAACCAAA TATTGACCTA 
CCGAGTTGTT ATAAGAGCAA TTCAAGAAAA AAACCTGATC AGAGTTTTGG AGGTGGAAAA 
TCATCTAGCC AGAGTGAGCC CAATTCTAAT CTTGAGAAGA TTCACAATGG AGTAATACCT 
TTTGATGATA ATCAGTGTGG AAACGTTTTT AGAAATACAC AATCCCTTAT TCAATATCAG 
AATGTGGAAA CTAAAGAGAA AAGCTGTGTA TGTGTTACAT GTGGAAAAGC CTTTGCTAAG 
AAGTCACAAC TCATTGTACA TCAAAGAATT CATACTGGAA AGAAACCATA TGATTGTGGT 
GCATGCGGAA AAGCCTTCAG TGAGAAGTTT CATCTTGTTG TACATCAGAG AACTCATACT 
GGGGAGAAAC CTTATGATTG TTCTGAATGT GGAAAAGCCT TCTCTCAGAA ATCGTCCCTT 
ATTATACATC AGAGAGTTCA CACTGGGGAA AAACCCTATG AATGTAGTGA ATGCGGGAAA 
GCCTTCTCCC AGAAATCACC CCTCATTATA CATCAGAGAA TACATACTGG GGAAAAACCC 
TATGAATGTA GAGAGTGTGG GAAGGCCTTT TCCCAGAAGT CACAGCTGAT TATACACCAC 
AGAGCTCATA CTGGAGAGAA GCCGTATGAG TGTACCGAAT GTGGGAAAGC CTTCTGTGAG 
AAGTCCCACC TCATTATACA TAAAAGAATT CACACTGGTG AGAAACCCTA CAAATGTGCT 
CAATGTGAGG AAGCCTTCAG CAGGAAGACA GAACTCATTA CACATCAGTT AGTTCATACT 
GGGGAAAAAC CTTATGAATG TACTGAATGT GGAAAGACAT TCTCCCGCAA GTCACAGCTC 
ATCATACATC AGAGAACACA TACTGGAGAA AAACCCTATA AATGTAGTGA ATGTGGCAAA 
GCCTTCTGCC AGAAGTCACA TCTCATTGGA CATCAGAGAA TTCACACAGG AGAAAAACCT 
TATATATGTA CTGAATGTGG GAAAGCCTTC TCTCAGAAGT CCCACCTTCC GGGACACCAG 
CGAATTCATA CAGGAGAGAA ACCTTACATA TGTGCTGAAT GTGGAAAGGC CTTTTCTCAG 
AAGTCAGACC TTGTTTTACA TCAGAGGATT CATACTGGGG AAAGACCCTA TCAATGTGCT 
ATATGTGGGA AGGCCTTCAT CCAGAAGTCA CAACTAACTG TACACCAGAG AATTCACACA 
GTGGTAAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_006714869.1

Translation

MMKSQGLVSFKDVAVDFTQEEWQQLDPSQRTLYRDVMLENYSHLVSMGYPVSKPDVISKLEQGEEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSILKVCQGDGQLQRFLENQDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNLKPNIDLPSCYKSNSRKKPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVETKEKSCVCVTCGKAFAKKSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAFSQKSSLIIHQRVHTGEKPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHTGEKPYECTECGKAFCEKSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTFSRKSQLIIHQRTHTGEKPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHTGEKPYICAECGKAFSQKSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS

Target ORF information:

RefSeq Version	XM_006714806.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714806.1

ORF Insert Sequence:

ATGATGAAGT CCCAGGGGTT AGTATCATTC AAGGATGTGG CTGTGGATTT CACCCAGGAG 
GAGTGGCAGC AACTTGACCC TTCTCAGAGG ACCCTGTACA GGGATGTGAT GCTGGAGAAC 
TACAGCCACC TGGTCTCAAT GGGGTATCCA GTTTCCAAAC CAGATGTCAT CTCCAAGTTG 
GAACAAGGAG AAGAGCCATG GATCATAAAG GGAGACATAT CAAATTGGAT CTATCCAGAT 
GAATATCAGG CAGATGGGAG ACAAGACAGG AAGAGTAACC TTCACAACTC CCAGTCATGT 
ATTTTGGGGA CAGTTTCCTT CCATCATAAG ATACTGAAAG GAGTCACAAG GGATGGTTCA 
TTGTGCTCCA TTTTAAAAGT CTGTCAAGGT GATGGTCAGC TGCAGAGATT TCTAGAGAAT 
CAAGACAAAC TCTTCAGGCA GGTCACATTT GTTAACAGCA AAACAGTGAC TGAGGCATCA 
GGGCATAAAT ATAATCCACT GGGGAAAATA TTTCAAGAGT GCATAGAAAC AGATATATCA 
ATACAGAGAT TCCATAAATA TGATGCTTTT AAAAAGAACT TAAAACCAAA TATTGACCTA 
CCGAGTTGTT ATAAGAGCAA TTCAAGAAAA AAACCTGATC AGAGTTTTGG AGGTGGAAAA 
TCATCTAGCC AGAGTGAGCC CAATTCTAAT CTTGAGAAGA TTCACAATGG AGTAATACCT 
TTTGATGATA ATCAGTGTGG AAACGTTTTT AGAAATACAC AATCCCTTAT TCAATATCAG 
AATGTGGAAA CTAAAGAGAA AAGCTGTGTA TGTGTTACAT GTGGAAAAGC CTTTGCTAAG 
AAGTCACAAC TCATTGTACA TCAAAGAATT CATACTGGAA AGAAACCATA TGATTGTGGT 
GCATGCGGAA AAGCCTTCAG TGAGAAGTTT CATCTTGTTG TACATCAGAG AACTCATACT 
GGGGAGAAAC CTTATGATTG TTCTGAATGT GGAAAAGCCT TCTCTCAGAA ATCGTCCCTT 
ATTATACATC AGAGAGTTCA CACTGGGGAA AAACCCTATG AATGTAGTGA ATGCGGGAAA 
GCCTTCTCCC AGAAATCACC CCTCATTATA CATCAGAGAA TACATACTGG GGAAAAACCC 
TATGAATGTA GAGAGTGTGG GAAGGCCTTT TCCCAGAAGT CACAGCTGAT TATACACCAC 
AGAGCTCATA CTGGAGAGAA GCCGTATGAG TGTACCGAAT GTGGGAAAGC CTTCTGTGAG 
AAGTCCCACC TCATTATACA TAAAAGAATT CACACTGGTG AGAAACCCTA CAAATGTGCT 
CAATGTGAGG AAGCCTTCAG CAGGAAGACA GAACTCATTA CACATCAGTT AGTTCATACT 
GGGGAAAAAC CTTATGAATG TACTGAATGT GGAAAGACAT TCTCCCGCAA GTCACAGCTC 
ATCATACATC AGAGAACACA TACTGGAGAA AAACCCTATA AATGTAGTGA ATGTGGCAAA 
GCCTTCTGCC AGAAGTCACA TCTCATTGGA CATCAGAGAA TTCACACAGG AGAAAAACCT 
TATATATGTA CTGAATGTGG GAAAGCCTTC TCTCAGAAGT CCCACCTTCC GGGACACCAG 
CGAATTCATA CAGGAGAGAA ACCTTACATA TGTGCTGAAT GTGGAAAGGC CTTTTCTCAG 
AAGTCAGACC TTGTTTTACA TCAGAGGATT CATACTGGGG AAAGACCCTA TCAATGTGCT 
ATATGTGGGA AGGCCTTCAT CCAGAAGTCA CAACTAACTG TACACCAGAG AATTCACACA 
GTGGTAAAAT CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF300 ( XM_011537702.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536004.1 Homo sapiens zinc finger protein 300 (ZNF300), transcript variant X1, mRNA.

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CloneID	OHu39914
Clone ID Related Accession (Same CDS sequence)	XM_011537702.2
Accession Version	XM_011537702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein 300 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCTA TTGTCCAGCT TTATCCACAG GGGTTAGTAT CATTCAAGGA TGTGGCTGTG 
GATTTCACCC AGGAGGAGTG GCAGCAACTT GACCCTTCTC AGAGGACCCT GTACAGGGAT 
GTGATGCTGG AGAACTACAG CCACCTGGTC TCAATGGGGT ATCCAGTTTC CAAACCAGAT 
GTCATCTCCA AGTTGGAACA AGGAGAAGAG CCATGGATCA TAAAGGGAGA CATATCAAAT 
TGGATCTATC CAGATGAATA TCAGGCAGAT GGGAGACAAG ACAGGAAGAG TAACCTTCAC 
AACTCCCAGT CATGTATTTT GGGGACAGTT TCCTTCCATC ATAAGATACT GAAAGGAGTC 
ACAAGGGATG GTTCATTGTG CTCCATTTTA AAAGTCTGTC AAGGTGATGG TCAGCTGCAG 
AGATTTCTAG AGAATCAAGA CAAACTCTTC AGGCAGGTCA CATTTGTTAA CAGCAAAACA 
GTGACTGAGG CATCAGGGCA TAAATATAAT CCACTGGGGA AAATATTTCA AGAGTGCATA 
GAAACAGATA TATCAATACA GAGATTCCAT AAATATGATG CTTTTAAAAA GAACTTAAAA 
CCAAATATTG ACCTACCGAG TTGTTATAAG AGCAATTCAA GAAAAAAACC TGATCAGAGT 
TTTGGAGGTG GAAAATCATC TAGCCAGAGT GAGCCCAATT CTAATCTTGA GAAGATTCAC 
AATGGAGTAA TACCTTTTGA TGATAATCAG TGTGGAAACG TTTTTAGAAA TACACAATCC 
CTTATTCAAT ATCAGAATGT GGAAACTAAA GAGAAAAGCT GTGTATGTGT TACATGTGGA 
AAAGCCTTTG CTAAGAAGTC ACAACTCATT GTACATCAAA GAATTCATAC TGGAAAGAAA 
CCATATGATT GTGGTGCATG CGGAAAAGCC TTCAGTGAGA AGTTTCATCT TGTTGTACAT 
CAGAGAACTC ATACTGGGGA GAAACCTTAT GATTGTTCTG AATGTGGAAA AGCCTTCTCT 
CAGAAATCGT CCCTTATTAT ACATCAGAGA GTTCACACTG GGGAAAAACC CTATGAATGT 
AGTGAATGCG GGAAAGCCTT CTCCCAGAAA TCACCCCTCA TTATACATCA GAGAATACAT 
ACTGGGGAAA AACCCTATGA ATGTAGAGAG TGTGGGAAGG CCTTTTCCCA GAAGTCACAG 
CTGATTATAC ACCACAGAGC TCATACTGGA GAGAAGCCGT ATGAGTGTAC CGAATGTGGG 
AAAGCCTTCT GTGAGAAGTC CCACCTCATT ATACATAAAA GAATTCACAC TGGTGAGAAA 
CCCTACAAAT GTGCTCAATG TGAGGAAGCC TTCAGCAGGA AGACAGAACT CATTACACAT 
CAGTTAGTTC ATACTGGGGA AAAACCTTAT GAATGTACTG AATGTGGAAA GACATTCTCC 
CGCAAGTCAC AGCTCATCAT ACATCAGAGA ACACATACTG GAGAAAAACC CTATAAATGT 
AGTGAATGTG GCAAAGCCTT CTGCCAGAAG TCACATCTCA TTGGACATCA GAGAATTCAC 
ACAGGAGAAA AACCTTATAT ATGTACTGAA TGTGGGAAAG CCTTCTCTCA GAAGTCCCAC 
CTTCCGGGAC ACCAGCGAAT TCATACAGGA GAGAAACCTT ACATATGTGC TGAATGTGGA 
AAGGCCTTTT CTCAGAAGTC AGACCTTGTT TTACATCAGA GGATTCATAC TGGGGAAAGA 
CCCTATCAAT GTGCTATATG TGGGAAGGCC TTCATCCAGA AGTCACAACT AACTGTACAC 
CAGAGAATTC ACACAGTGGT AAAATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536004.1
CDS	562..2391
Translation	MLPIVQLYPQGLVSFKDVAVDFTQEEWQQLDPSQRTLYRDVMLENYSHLVSMGYPVSKPDVISKLEQGEEPWIIKGDISNWIYPDEYQADGRQDRKSNLHNSQSCILGTVSFHHKILKGVTRDGSLCSILKVCQGDGQLQRFLENQDKLFRQVTFVNSKTVTEASGHKYNPLGKIFQECIETDISIQRFHKYDAFKKNLKPNIDLPSCYKSNSRKKPDQSFGGGKSSSQSEPNSNLEKIHNGVIPFDDNQCGNVFRNTQSLIQYQNVETKEKSCVCVTCGKAFAKKSQLIVHQRIHTGKKPYDCGACGKAFSEKFHLVVHQRTHTGEKPYDCSECGKAFSQKSSLIIHQRVHTGEKPYECSECGKAFSQKSPLIIHQRIHTGEKPYECRECGKAFSQKSQLIIHHRAHTGEKPYECTECGKAFCEKSHLIIHKRIHTGEKPYKCAQCEEAFSRKTELITHQLVHTGEKPYECTECGKTFSRKSQLIIHQRTHTGEKPYKCSECGKAFCQKSHLIGHQRIHTGEKPYICTECGKAFSQKSHLPGHQRIHTGEKPYICAECGKAFSQKSDLVLHQRIHTGERPYQCAICGKAFIQKSQLTVHQRIHTVVKS

Target ORF information:

RefSeq Version	XM_011537702.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 300 (ZNF300), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537702.2

ORF Insert Sequence:

ATGCTTCCTA TTGTCCAGCT TTATCCACAG GGGTTAGTAT CATTCAAGGA TGTGGCTGTG 
GATTTCACCC AGGAGGAGTG GCAGCAACTT GACCCTTCTC AGAGGACCCT GTACAGGGAT 
GTGATGCTGG AGAACTACAG CCACCTGGTC TCAATGGGGT ATCCAGTTTC CAAACCAGAT 
GTCATCTCCA AGTTGGAACA AGGAGAAGAG CCATGGATCA TAAAGGGAGA CATATCAAAT 
TGGATCTATC CAGATGAATA TCAGGCAGAT GGGAGACAAG ACAGGAAGAG TAACCTTCAC 
AACTCCCAGT CATGTATTTT GGGGACAGTT TCCTTCCATC ATAAGATACT GAAAGGAGTC 
ACAAGGGATG GTTCATTGTG CTCCATTTTA AAAGTCTGTC AAGGTGATGG TCAGCTGCAG 
AGATTTCTAG AGAATCAAGA CAAACTCTTC AGGCAGGTCA CATTTGTTAA CAGCAAAACA 
GTGACTGAGG CATCAGGGCA TAAATATAAT CCACTGGGGA AAATATTTCA AGAGTGCATA 
GAAACAGATA TATCAATACA GAGATTCCAT AAATATGATG CTTTTAAAAA GAACTTAAAA 
CCAAATATTG ACCTACCGAG TTGTTATAAG AGCAATTCAA GAAAAAAACC TGATCAGAGT 
TTTGGAGGTG GAAAATCATC TAGCCAGAGT GAGCCCAATT CTAATCTTGA GAAGATTCAC 
AATGGAGTAA TACCTTTTGA TGATAATCAG TGTGGAAACG TTTTTAGAAA TACACAATCC 
CTTATTCAAT ATCAGAATGT GGAAACTAAA GAGAAAAGCT GTGTATGTGT TACATGTGGA 
AAAGCCTTTG CTAAGAAGTC ACAACTCATT GTACATCAAA GAATTCATAC TGGAAAGAAA 
CCATATGATT GTGGTGCATG CGGAAAAGCC TTCAGTGAGA AGTTTCATCT TGTTGTACAT 
CAGAGAACTC ATACTGGGGA GAAACCTTAT GATTGTTCTG AATGTGGAAA AGCCTTCTCT 
CAGAAATCGT CCCTTATTAT ACATCAGAGA GTTCACACTG GGGAAAAACC CTATGAATGT 
AGTGAATGCG GGAAAGCCTT CTCCCAGAAA TCACCCCTCA TTATACATCA GAGAATACAT 
ACTGGGGAAA AACCCTATGA ATGTAGAGAG TGTGGGAAGG CCTTTTCCCA GAAGTCACAG 
CTGATTATAC ACCACAGAGC TCATACTGGA GAGAAGCCGT ATGAGTGTAC CGAATGTGGG 
AAAGCCTTCT GTGAGAAGTC CCACCTCATT ATACATAAAA GAATTCACAC TGGTGAGAAA 
CCCTACAAAT GTGCTCAATG TGAGGAAGCC TTCAGCAGGA AGACAGAACT CATTACACAT 
CAGTTAGTTC ATACTGGGGA AAAACCTTAT GAATGTACTG AATGTGGAAA GACATTCTCC 
CGCAAGTCAC AGCTCATCAT ACATCAGAGA ACACATACTG GAGAAAAACC CTATAAATGT 
AGTGAATGTG GCAAAGCCTT CTGCCAGAAG TCACATCTCA TTGGACATCA GAGAATTCAC 
ACAGGAGAAA AACCTTATAT ATGTACTGAA TGTGGGAAAG CCTTCTCTCA GAAGTCCCAC 
CTTCCGGGAC ACCAGCGAAT TCATACAGGA GAGAAACCTT ACATATGTGC TGAATGTGGA 
AAGGCCTTTT CTCAGAAGTC AGACCTTGTT TTACATCAGA GGATTCATAC TGGGGAAAGA 
CCCTATCAAT GTGCTATATG TGGGAAGGCC TTCATCCAGA AGTCACAACT AACTGTACAC 
CAGAGAATTC ACACAGTGGT AAAATCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZNF300P1 encodes a lincRNA that regulates cell polarity and is epigenetically silenced in type II epithelial ovarian cancer.
Molecular cancer133(2014 Jan)
Gloss B,Moran-Jones K,Lin V,Gonzalez M,Scurry J,Hacker NF,Sutherland RL,Clark SJ,Samimi G

Novel activity of KRAB domain that functions to reinforce nuclear localization of KRAB-containing zinc finger proteins by interacting with KAP1.
Cellular and molecular life sciences : CMLS70(20)3947-58(2013 Oct)
Wang W,Cai J,Wu Y,Hu L,Chen Z,Hu J,Chen Z,Li W,Guo M,Huang Z

Host-microbe interactions have shaped the genetic architecture of inflammatory bowel disease.
Nature491(7422)119-24(2012 Nov)
Jostins L,Ripke S,Weersma RK,Duerr RH,McGovern DP,Hui KY,Lee JC,Schumm LP,Sharma Y,Anderson CA,Essers J,Mitrovic M,Ning K,Cleynen I,Theatre E,Spain SL,Raychaudhuri S,Goyette P,Wei Z,Abraham C,Achkar JP,Ahmad T,Amininejad L,Ananthakrishnan AN,Andersen V,Andrews JM,Baidoo L,Balschun T,Bampton PA,Bitton A,Boucher G,Brand S,B?ning C,Cohain A,Cichon S,D'Amato M,De Jong D,Devaney KL,Dubinsky M,Edwards C,Ellinghaus D,Ferguson LR,Franchimont D,Fransen K,Gearry R,Georges M,Gieger C,Glas J,Haritunians T,Hart A,Hawkey C,Hedl M,Hu X,Karlsen TH,Kupcinskas L,Kugathasan S,Latiano A,Laukens D,Lawrance IC,Lees CW,Louis E,Mahy G,Mansfield J,Morgan AR,Mowat C,Newman W,Palmieri O,Ponsioen CY,Potocnik U,Prescott NJ,Regueiro M,Rotter JI,Russell RK,Sanderson JD,Sans M,Satsangi J,Schreiber S,Simms LA,Sventoraityte J,Targan SR,Taylor KD,Tremelling M,Verspaget HW,De Vos M,Wijmenga C,Wilson DC,Winkelmann J,Xavier RJ,Zeissig S,Zhang B,Zhang CK,Zhao H,,Silverberg MS,Annese V,Hakonarson H,Brant SR,Radford-Smith G,Mathew CG,Rioux JD,Schadt EE,Daly MJ,Franke A,Parkes M,Vermeire S,Barrett JC,Cho JH

Overexpression of the human ZNF300 gene enhances growth and metastasis of cancer cells through activating NF-kB pathway.
Journal of cellular and molecular medicine16(5)1134-45(2012 May)
Wang T,Wang XG,Xu JH,Wu XP,Qiu HL,Yi H,Li WX

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ZNF300 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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