DDX21 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX21
Entrez Gene ID	9188
Full Name	DExD-box helicase 21
Synonyms	GUA,GURDB,RH-II/GU,RH-II/GuA
General protein information	Preferred Names DExD-box helicase 21 Names nucleolar RNA helicase 2 DEAD (Asp-Glu-Ala-Asp) box helicase 21 DEAD (Asp-Glu-Ala-Asp) box polypeptide 21 DEAD box protein 21 DEAD-box helicase 21 DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 Gu protein RH II/Gu RNA helicase II/Gu alpha gu-alpha nucleolar RNA helicase Gu nucleolar RNA helicase II
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q22.1
Summary	DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is an antigen recognized by autoimmune antibodies from a patient with watermelon stomach disease. This protein unwinds double-stranded RNA, folds single-stranded RNA, and may play important roles in ribosomal RNA biogenesis, RNA editing, RNA transport, and general transcription. [provided by RefSeq, Jul 2008].
Disorder MIM:	606357

mRNA and Protein(s)

mRNA	Protein	Name
NM_001256910.1	NP_001243839.1	nucleolar RNA helicase 2 isoform 2
XM_017016910.2	XP_016872399.1	nucleolar RNA helicase 2 isoform X2
NM_004728.3	NP_004719.2	nucleolar RNA helicase 2 isoform 1
XM_017016910.1	XP_016872399.1	nucleolar RNA helicase 2 isoform X2
XM_011540336.1	XP_011538638.1	nucleolar RNA helicase 2 isoform X1
NM_004728.4	NP_004719.2	nucleolar RNA helicase 2 isoform 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ddx21	NP_001039224.1
Bos taurus (cattle)	DDX21	NP_001076996.1
Pan troglodytes (chimpanzee)	DDX21	XP_507825.2
	DDX21	XP_001110939.1
Gallus gallus (chicken)	DDX50	XP_001232052.1
Danio rerio (zebrafish)	ddx21	NP_001120807.1
Homo sapiens (human)	DDX21	NP_004719.2
Canis lupus familiaris (dog)	DDX21	XP_851746.2
Mus musculus (house mouse)	Ddx21	NP_062426.2
Rattus norvegicus (Norway rat)	Ddx21	NP_001032278.1
Arabidopsis thaliana (thale cress)	PRH75	NP_201025.1
Oryza sativa (rice)	Os09g0520700	NP_001063702.1

Gene Ontology
Bibliography

Related articles in PubMed

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.
Xing YH, Yao RW, Zhang Y, Guo CJ, Jiang S, Xu G, Dong R, Yang L, Chen LL
Cell169(4)664-678.e16(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

DDX21 can suppress the expression of proteins with G4 qudruplexes in the 3 UTR of its mRNA.
Title: Human DDX21 binds and unwinds RNA guanine quadruplexes.

Results report the biogenesis and function of a box H/ACA snoRNA-ended sno-lncRNA, referred to SLERT (snoRNA-ended lncRNA enhances pre-ribosomal RNA transcription). SLERT is different from Prader-Willi Syndrome (PWS) sno-lncRNAs and plays a crucial role in rRNA biogenesis by dislodging a previously unknown clamp of DDX21 ring-shaped arrangements on Pol I complexes, thereby liberating Pol I for active rRNA transcription.
Title: SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

In dengue virus infected cells, DDX21 translocates from nucleus to cytoplasm to active the innate immune response and thus inhibits DENV replication in the early stages of infection.
Title: DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection.

results uncover the multifaceted role of DDX21 in multiple steps of ribosome biogenesis, and provide evidence implicating a mammalian RNA helicase in RNA modification and Pol II elongation control
Title: RNA helicase DDX21 coordinates transcription and ribosomal RNA processing.

Identification of several late-acting snoRNAs that bind pre-40S particles in human cells and show that their association and function in pre-40S complexes is regulated by the RNA helicase DDX21.
Title: The association of late-acting snoRNPs with human pre-ribosomal complexes requires the RNA helicase DDX21.

The following DDX21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25158	NM_001256910.1 Latest version!	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu25165	NM_004728.4 Latest version!	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu62586	XM_011540336.1 Latest version!	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu91810	XM_017016910.2 Latest version!	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu91810	XM_017016910.1	Homo sapiens DEAD-box helicase 21 (DDX21), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu25165	NM_004728.3	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX21 ( NM_001256910.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243839.1 Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25158
Clone ID Related Accession (Same CDS sequence)	NM_001256910.1
Accession Version	NM_001256910.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	nucleolar RNA helicase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL359844.15 and AA446317.1. Transcript Variant: This variant (2) differs in the 5'UTR and has multiple coding region differences, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648405.1, CR749598.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTCTC CTAAATCCAA AAAGGCAAAA AAGAAAGAGG AGCCATCTCA AAATGACATT 
TCTCCTAAAA CCAAAAGTTT GAGAAAGAAA AAGGAGCCCA TTGAAAAGAA AGTGGTTTCT 
TCTAAAACCA AAAAAGTGAC AAAAAATGAG GAGCCTTCTG AGGAAGAAAT AGATGCTCCT 
AAGCCCAAGA AGATGAAGAA AGAAAAGGAA ATGAATGGAG AAACTAGAGA GAAAAGCCCC 
AAACTGAAGA ATGGATTTCC TCATCCTGAA CCGGACTGTA ACCCCAGTGA AGCTGCCAGT 
GAAGAAAGTA ACAGTGAGAT AGAGCAGGAA ATACCTGTGG AACAAAAAGA AGGCGCTTTC 
TCTAATTTTC CCATATCTGA AGAAACTATT AAACTTCTCA AAGGCCGAGG AGTGACCTTC 
CTATTTCCTA TACAAGCAAA GACATTCCAT CATGTTTACA GCGGGAAGGA CTTAATTGCA 
CAGGCACGGA CAGGAACTGG GAAGACATTC TCCTTTGCCA TCCCTTTGAT TGAGAAACTT 
CATGGGGAAC TGCAAGACAG GAAGAGAGGC CGTGCCCCTC AGGTACTGGT TCTTGCACCT 
ACAAGAGAGT TGGCAAATCA AGTAAGCAAA GACTTCAGTG ACATCACAAA AAAGCTGTCA 
GTGGCTTGTT TTTATGGTGG AACTCCCTAT GGAGGTCAAT TTGAACGCAT GAGGAATGGG 
ATTGATATCC TGGTTGGAAC ACCAGGTCGT ATCAAAGACC ACATACAGAA TGGCAAACTA 
GATCTCACCA AACTTAAGCA TGTTGTCCTG GATGAAGTGG ACCAGATGTT GGATATGGGA 
TTTGCTGATC AAGTGGAAGA GATTTTAAGT GTGGCATACA AGAAAGATTC TGAAGACAAT 
CCCCAAACAT TGCTTTTTTC TGCAACTTGC CCTCATTGGG TATTTAATGT TGCCAAGAAA 
TACATGAAAT CTACATATGA ACAGGTGGAC CTGATTGGTA AAAAGACTCA GAAAACGGCA 
ATAACTGTGG AGCATCTGGC TATTAAGTGC CACTGGACTC AGAGGGCAGC AGTTATTGGG 
GATGTCATCC GAGTATATAG TGGTCATCAA GGACGCACTA TCATCTTTTG TGAAACCAAG 
AAAGAAGCCC AGGAGCTGTC CCAGAATTCA GCTATAAAGC AGGATGCTCA GTCCTTGCAT 
GGAGACATTC CACAGAAGCA AAGGGAAATC ACCCTGAAAG GTTTTAGAAA TGGTAGTTTT 
GGAGTTTTGG TGGCAACCAA TGTTGCTGCA CGTGGGTTAG ACATCCCTGA GGTTGATTTG 
GTTATACAAA GCTCTCCACC AAAGGATGTA GAGTCCTACA TTCATCGATC CGGGCGGACA 
GGCAGAGCTG GAAGGACGGG GGTGTGCATC TGCTTTTATC AGCACAAGGA AGAATATCAG 
TTAGTACAAG TGGAGCAAAA AGCGGGAATT AAGTTCAAAC GAATAGGTGT TCCTTCTGCA 
ACAGAAATAA TAAAAGCTTC CAGCAAAGAT GCCATCAGGC TTTTGGATTC CGTGCCTCCC 
ACTGCCATTA GTCACTTCAA ACAATCAGCT GAGAAGCTGA TAGAGGAGAA GGGAGCTGTG 
GAAGCTCTGG CAGCAGCACT GGCCCATATT TCAGGTGCCA CGTCCGTAGA CCAGCGCTCC 
TTGATCAACT CAAATGTGGG TTTTGTGACC ATGATCTTGC AGTGCTCAAT TGAAATGCCA 
AATATTAGTT ATGCTTGGAA AGAACTTAAA GAGCAGCTGG GCGAGGAGAT TGATTCCAAA 
GTGAAGGGAA TGGTTTTTCT CAAAGGAAAG CTGGGTGTTT GCTTTGATGT ACCTACCGCA 
TCAGTAACAG AAATACAGGA GAAATGGCAT GATTCACGAC GCTGGCAGCT CTCTGTGGCC 
ACAGAGCAAC CAGAACTGGA AGGACCACGG GAAGGATATG GAGGCTTCAG GGGACAGCGG 
GAAGGCAGTC GAGGCTTCAG GGGACAGCGG GACGGAAACA GAAGATTCAG AGGACAGCGG 
GAAGGCAGTA GAGGCCCGAG AGGACAGCGA TCAGGAGGTG GCAACAAAAG TAACAGATCC 
CAAAACAAAG GCCAGAAGCG GAGTTTCAGT AAAGCATTTG GTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243839.1
CDS	427..2574
Translation	MNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	NM_001256910.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256910.1

ORF Insert Sequence:

ATGAATTCTC CTAAATCCAA AAAGGCAAAA AAGAAAGAGG AGCCATCTCA AAATGACATT 
TCTCCTAAAA CCAAAAGTTT GAGAAAGAAA AAGGAGCCCA TTGAAAAGAA AGTGGTTTCT 
TCTAAAACCA AAAAAGTGAC AAAAAATGAG GAGCCTTCTG AGGAAGAAAT AGATGCTCCT 
AAGCCCAAGA AGATGAAGAA AGAAAAGGAA ATGAATGGAG AAACTAGAGA GAAAAGCCCC 
AAACTGAAGA ATGGATTTCC TCATCCTGAA CCGGACTGTA ACCCCAGTGA AGCTGCCAGT 
GAAGAAAGTA ACAGTGAGAT AGAGCAGGAA ATACCTGTGG AACAAAAAGA AGGCGCTTTC 
TCTAATTTTC CCATATCTGA AGAAACTATT AAACTTCTCA AAGGCCGAGG AGTGACCTTC 
CTATTTCCTA TACAAGCAAA GACATTCCAT CATGTTTACA GCGGGAAGGA CTTAATTGCA 
CAGGCACGGA CAGGAACTGG GAAGACATTC TCCTTTGCCA TCCCTTTGAT TGAGAAACTT 
CATGGGGAAC TGCAAGACAG GAAGAGAGGC CGTGCCCCTC AGGTACTGGT TCTTGCACCT 
ACAAGAGAGT TGGCAAATCA AGTAAGCAAA GACTTCAGTG ACATCACAAA AAAGCTGTCA 
GTGGCTTGTT TTTATGGTGG AACTCCCTAT GGAGGTCAAT TTGAACGCAT GAGGAATGGG 
ATTGATATCC TGGTTGGAAC ACCAGGTCGT ATCAAAGACC ACATACAGAA TGGCAAACTA 
GATCTCACCA AACTTAAGCA TGTTGTCCTG GATGAAGTGG ACCAGATGTT GGATATGGGA 
TTTGCTGATC AAGTGGAAGA GATTTTAAGT GTGGCATACA AGAAAGATTC TGAAGACAAT 
CCCCAAACAT TGCTTTTTTC TGCAACTTGC CCTCATTGGG TATTTAATGT TGCCAAGAAA 
TACATGAAAT CTACATATGA ACAGGTGGAC CTGATTGGTA AAAAGACTCA GAAAACGGCA 
ATAACTGTGG AGCATCTGGC TATTAAGTGC CACTGGACTC AGAGGGCAGC AGTTATTGGG 
GATGTCATCC GAGTATATAG TGGTCATCAA GGACGCACTA TCATCTTTTG TGAAACCAAG 
AAAGAAGCCC AGGAGCTGTC CCAGAATTCA GCTATAAAGC AGGATGCTCA GTCCTTGCAT 
GGAGACATTC CACAGAAGCA AAGGGAAATC ACCCTGAAAG GTTTTAGAAA TGGTAGTTTT 
GGAGTTTTGG TGGCAACCAA TGTTGCTGCA CGTGGGTTAG ACATCCCTGA GGTTGATTTG 
GTTATACAAA GCTCTCCACC AAAGGATGTA GAGTCCTACA TTCATCGATC CGGGCGGACA 
GGCAGAGCTG GAAGGACGGG GGTGTGCATC TGCTTTTATC AGCACAAGGA AGAATATCAG 
TTAGTACAAG TGGAGCAAAA AGCGGGAATT AAGTTCAAAC GAATAGGTGT TCCTTCTGCA 
ACAGAAATAA TAAAAGCTTC CAGCAAAGAT GCCATCAGGC TTTTGGATTC CGTGCCTCCC 
ACTGCCATTA GTCACTTCAA ACAATCAGCT GAGAAGCTGA TAGAGGAGAA GGGAGCTGTG 
GAAGCTCTGG CAGCAGCACT GGCCCATATT TCAGGTGCCA CGTCCGTAGA CCAGCGCTCC 
TTGATCAACT CAAATGTGGG TTTTGTGACC ATGATCTTGC AGTGCTCAAT TGAAATGCCA 
AATATTAGTT ATGCTTGGAA AGAACTTAAA GAGCAGCTGG GCGAGGAGAT TGATTCCAAA 
GTGAAGGGAA TGGTTTTTCT CAAAGGAAAG CTGGGTGTTT GCTTTGATGT ACCTACCGCA 
TCAGTAACAG AAATACAGGA GAAATGGCAT GATTCACGAC GCTGGCAGCT CTCTGTGGCC 
ACAGAGCAAC CAGAACTGGA AGGACCACGG GAAGGATATG GAGGCTTCAG GGGACAGCGG 
GAAGGCAGTC GAGGCTTCAG GGGACAGCGG GACGGAAACA GAAGATTCAG AGGACAGCGG 
GAAGGCAGTA GAGGCCCGAG AGGACAGCGA TCAGGAGGTG GCAACAAAAG TAACAGATCC 
CAAAACAAAG GCCAGAAGCG GAGTTTCAGT AAAGCATTTG GTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX21 ( NM_004728.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004719.2 Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25165
Clone ID Related Accession (Same CDS sequence)	NM_004728.3 , NM_004728.4
Accession Version	NM_004728.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	nucleolar RNA helicase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW408689.1, BC008071.2, AL359844.15 and AA446317.1. On Mar 7, 2012 this sequence version replaced NM_004728.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.2314.1, SRR1803615.50693.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGGGAA AACTCCGTAG TGACGCTGGT TTGGAATCAG ACACCGCAAT GAAAAAAGGG 
GAGACACTGC GAAAGCAAAC CGAGGAGAAA GAGAAAAAAG AGAAGCCAAA ATCTGATAAG 
ACTGAAGAGA TAGCAGAAGA GGAAGAAACT GTTTTCCCCA AAGCTAAACA AGTTAAAAAG 
AAAGCAGAGC CTTCTGAAGT TGACATGAAT TCTCCTAAAT CCAAAAAGGC AAAAAAGAAA 
GAGGAGCCAT CTCAAAATGA CATTTCTCCT AAAACCAAAA GTTTGAGAAA GAAAAAGGAG 
CCCATTGAAA AGAAAGTGGT TTCTTCTAAA ACCAAAAAAG TGACAAAAAA TGAGGAGCCT 
TCTGAGGAAG AAATAGATGC TCCTAAGCCC AAGAAGATGA AGAAAGAAAA GGAAATGAAT 
GGAGAAACTA GAGAGAAAAG CCCCAAACTG AAGAATGGAT TTCCTCATCC TGAACCGGAC 
TGTAACCCCA GTGAAGCTGC CAGTGAAGAA AGTAACAGTG AGATAGAGCA GGAAATACCT 
GTGGAACAAA AAGAAGGCGC TTTCTCTAAT TTTCCCATAT CTGAAGAAAC TATTAAACTT 
CTCAAAGGCC GAGGAGTGAC CTTCCTATTT CCTATACAAG CAAAGACATT CCATCATGTT 
TACAGCGGGA AGGACTTAAT TGCACAGGCA CGGACAGGAA CTGGGAAGAC ATTCTCCTTT 
GCCATCCCTT TGATTGAGAA ACTTCATGGG GAACTGCAAG ACAGGAAGAG AGGCCGTGCC 
CCTCAGGTAC TGGTTCTTGC ACCTACAAGA GAGTTGGCAA ATCAAGTAAG CAAAGACTTC 
AGTGACATCA CAAAAAAGCT GTCAGTGGCT TGTTTTTATG GTGGAACTCC CTATGGAGGT 
CAATTTGAAC GCATGAGGAA TGGGATTGAT ATCCTGGTTG GAACACCAGG TCGTATCAAA 
GACCACATAC AGAATGGCAA ACTAGATCTC ACCAAACTTA AGCATGTTGT CCTGGATGAA 
GTGGACCAGA TGTTGGATAT GGGATTTGCT GATCAAGTGG AAGAGATTTT AAGTGTGGCA 
TACAAGAAAG ATTCTGAAGA CAATCCCCAA ACATTGCTTT TTTCTGCAAC TTGCCCTCAT 
TGGGTATTTA ATGTTGCCAA GAAATACATG AAATCTACAT ATGAACAGGT GGACCTGATT 
GGTAAAAAGA CTCAGAAAAC GGCAATAACT GTGGAGCATC TGGCTATTAA GTGCCACTGG 
ACTCAGAGGG CAGCAGTTAT TGGGGATGTC ATCCGAGTAT ATAGTGGTCA TCAAGGACGC 
ACTATCATCT TTTGTGAAAC CAAGAAAGAA GCCCAGGAGC TGTCCCAGAA TTCAGCTATA 
AAGCAGGATG CTCAGTCCTT GCATGGAGAC ATTCCACAGA AGCAAAGGGA AATCACCCTG 
AAAGGTTTTA GAAATGGTAG TTTTGGAGTT TTGGTGGCAA CCAATGTTGC TGCACGTGGG 
TTAGACATCC CTGAGGTTGA TTTGGTTATA CAAAGCTCTC CACCAAAGGA TGTAGAGTCC 
TACATTCATC GATCCGGGCG GACAGGCAGA GCTGGAAGGA CGGGGGTGTG CATCTGCTTT 
TATCAGCACA AGGAAGAATA TCAGTTAGTA CAAGTGGAGC AAAAAGCGGG AATTAAGTTC 
AAACGAATAG GTGTTCCTTC TGCAACAGAA ATAATAAAAG CTTCCAGCAA AGATGCCATC 
AGGCTTTTGG ATTCCGTGCC TCCCACTGCC ATTAGTCACT TCAAACAATC AGCTGAGAAG 
CTGATAGAGG AGAAGGGAGC TGTGGAAGCT CTGGCAGCAG CACTGGCCCA TATTTCAGGT 
GCCACGTCCG TAGACCAGCG CTCCTTGATC AACTCAAATG TGGGTTTTGT GACCATGATC 
TTGCAGTGCT CAATTGAAAT GCCAAATATT AGTTATGCTT GGAAAGAACT TAAAGAGCAG 
CTGGGCGAGG AGATTGATTC CAAAGTGAAG GGAATGGTTT TTCTCAAAGG AAAGCTGGGT 
GTTTGCTTTG ATGTACCTAC CGCATCAGTA ACAGAAATAC AGGAGAAATG GCATGATTCA 
CGACGCTGGC AGCTCTCTGT GGCCACAGAG CAACCAGAAC TGGAAGGACC ACGGGAAGGA 
TATGGAGGCT TCAGGGGACA GCGGGAAGGC AGTCGAGGCT TCAGGGGACA GCGGGACGGA 
AACAGAAGAT TCAGAGGACA GCGGGAAGGC AGTAGAGGCC CGAGAGGACA GCGATCAGGA 
GGTGGCAACA AAAGTAACAG ATCCCAAAAC AAAGGCCAGA AGCGGAGTTT CAGTAAAGCA 
TTTGGTCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004719.2
CDS	104..2455
Translation	MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	NM_004728.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004728.3

ORF Insert Sequence:

ATGCCGGGAA AACTCCGTAG TGACGCTGGT TTGGAATCAG ACACCGCAAT GAAAAAAGGG 
GAGACACTGC GAAAGCAAAC CGAGGAGAAA GAGAAAAAAG AGAAGCCAAA ATCTGATAAG 
ACTGAAGAGA TAGCAGAAGA GGAAGAAACT GTTTTCCCCA AAGCTAAACA AGTTAAAAAG 
AAAGCAGAGC CTTCTGAAGT TGACATGAAT TCTCCTAAAT CCAAAAAGGC AAAAAAGAAA 
GAGGAGCCAT CTCAAAATGA CATTTCTCCT AAAACCAAAA GTTTGAGAAA GAAAAAGGAG 
CCCATTGAAA AGAAAGTGGT TTCTTCTAAA ACCAAAAAAG TGACAAAAAA TGAGGAGCCT 
TCTGAGGAAG AAATAGATGC TCCTAAGCCC AAGAAGATGA AGAAAGAAAA GGAAATGAAT 
GGAGAAACTA GAGAGAAAAG CCCCAAACTG AAGAATGGAT TTCCTCATCC TGAACCGGAC 
TGTAACCCCA GTGAAGCTGC CAGTGAAGAA AGTAACAGTG AGATAGAGCA GGAAATACCT 
GTGGAACAAA AAGAAGGCGC TTTCTCTAAT TTTCCCATAT CTGAAGAAAC TATTAAACTT 
CTCAAAGGCC GAGGAGTGAC CTTCCTATTT CCTATACAAG CAAAGACATT CCATCATGTT 
TACAGCGGGA AGGACTTAAT TGCACAGGCA CGGACAGGAA CTGGGAAGAC ATTCTCCTTT 
GCCATCCCTT TGATTGAGAA ACTTCATGGG GAACTGCAAG ACAGGAAGAG AGGCCGTGCC 
CCTCAGGTAC TGGTTCTTGC ACCTACAAGA GAGTTGGCAA ATCAAGTAAG CAAAGACTTC 
AGTGACATCA CAAAAAAGCT GTCAGTGGCT TGTTTTTATG GTGGAACTCC CTATGGAGGT 
CAATTTGAAC GCATGAGGAA TGGGATTGAT ATCCTGGTTG GAACACCAGG TCGTATCAAA 
GACCACATAC AGAATGGCAA ACTAGATCTC ACCAAACTTA AGCATGTTGT CCTGGATGAA 
GTGGACCAGA TGTTGGATAT GGGATTTGCT GATCAAGTGG AAGAGATTTT AAGTGTGGCA 
TACAAGAAAG ATTCTGAAGA CAATCCCCAA ACATTGCTTT TTTCTGCAAC TTGCCCTCAT 
TGGGTATTTA ATGTTGCCAA GAAATACATG AAATCTACAT ATGAACAGGT GGACCTGATT 
GGTAAAAAGA CTCAGAAAAC GGCAATAACT GTGGAGCATC TGGCTATTAA GTGCCACTGG 
ACTCAGAGGG CAGCAGTTAT TGGGGATGTC ATCCGAGTAT ATAGTGGTCA TCAAGGACGC 
ACTATCATCT TTTGTGAAAC CAAGAAAGAA GCCCAGGAGC TGTCCCAGAA TTCAGCTATA 
AAGCAGGATG CTCAGTCCTT GCATGGAGAC ATTCCACAGA AGCAAAGGGA AATCACCCTG 
AAAGGTTTTA GAAATGGTAG TTTTGGAGTT TTGGTGGCAA CCAATGTTGC TGCACGTGGG 
TTAGACATCC CTGAGGTTGA TTTGGTTATA CAAAGCTCTC CACCAAAGGA TGTAGAGTCC 
TACATTCATC GATCCGGGCG GACAGGCAGA GCTGGAAGGA CGGGGGTGTG CATCTGCTTT 
TATCAGCACA AGGAAGAATA TCAGTTAGTA CAAGTGGAGC AAAAAGCGGG AATTAAGTTC 
AAACGAATAG GTGTTCCTTC TGCAACAGAA ATAATAAAAG CTTCCAGCAA AGATGCCATC 
AGGCTTTTGG ATTCCGTGCC TCCCACTGCC ATTAGTCACT TCAAACAATC AGCTGAGAAG 
CTGATAGAGG AGAAGGGAGC TGTGGAAGCT CTGGCAGCAG CACTGGCCCA TATTTCAGGT 
GCCACGTCCG TAGACCAGCG CTCCTTGATC AACTCAAATG TGGGTTTTGT GACCATGATC 
TTGCAGTGCT CAATTGAAAT GCCAAATATT AGTTATGCTT GGAAAGAACT TAAAGAGCAG 
CTGGGCGAGG AGATTGATTC CAAAGTGAAG GGAATGGTTT TTCTCAAAGG AAAGCTGGGT 
GTTTGCTTTG ATGTACCTAC CGCATCAGTA ACAGAAATAC AGGAGAAATG GCATGATTCA 
CGACGCTGGC AGCTCTCTGT GGCCACAGAG CAACCAGAAC TGGAAGGACC ACGGGAAGGA 
TATGGAGGCT TCAGGGGACA GCGGGAAGGC AGTCGAGGCT TCAGGGGACA GCGGGACGGA 
AACAGAAGAT TCAGAGGACA GCGGGAAGGC AGTAGAGGCC CGAGAGGACA GCGATCAGGA 
GGTGGCAACA AAAGTAACAG ATCCCAAAAC AAAGGCCAGA AGCGGAGTTT CAGTAAAGCA 
TTTGGTCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX21 ( NM_004728.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004719.2 Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 1, mRNA.

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CloneID	OHu25165
Clone ID Related Accession (Same CDS sequence)	NM_004728.3 , NM_004728.4
Accession Version	NM_004728.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	nucleolar RNA helicase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DW408689.1, BC008071.2, AL359844.15 and AA446317.1. On Nov 22, 2018 this sequence version replaced NM_004728.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC009432.1, SRR1660803.2314.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000354185.9/ ENSP00000346120.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGGAA AACTCCGTAG TGACGCTGGT TTGGAATCAG ACACCGCAAT GAAAAAAGGG 
GAGACACTGC GAAAGCAAAC CGAGGAGAAA GAGAAAAAAG AGAAGCCAAA ATCTGATAAG 
ACTGAAGAGA TAGCAGAAGA GGAAGAAACT GTTTTCCCCA AAGCTAAACA AGTTAAAAAG 
AAAGCAGAGC CTTCTGAAGT TGACATGAAT TCTCCTAAAT CCAAAAAGGC AAAAAAGAAA 
GAGGAGCCAT CTCAAAATGA CATTTCTCCT AAAACCAAAA GTTTGAGAAA GAAAAAGGAG 
CCCATTGAAA AGAAAGTGGT TTCTTCTAAA ACCAAAAAAG TGACAAAAAA TGAGGAGCCT 
TCTGAGGAAG AAATAGATGC TCCTAAGCCC AAGAAGATGA AGAAAGAAAA GGAAATGAAT 
GGAGAAACTA GAGAGAAAAG CCCCAAACTG AAGAATGGAT TTCCTCATCC TGAACCGGAC 
TGTAACCCCA GTGAAGCTGC CAGTGAAGAA AGTAACAGTG AGATAGAGCA GGAAATACCT 
GTGGAACAAA AAGAAGGCGC TTTCTCTAAT TTTCCCATAT CTGAAGAAAC TATTAAACTT 
CTCAAAGGCC GAGGAGTGAC CTTCCTATTT CCTATACAAG CAAAGACATT CCATCATGTT 
TACAGCGGGA AGGACTTAAT TGCACAGGCA CGGACAGGAA CTGGGAAGAC ATTCTCCTTT 
GCCATCCCTT TGATTGAGAA ACTTCATGGG GAACTGCAAG ACAGGAAGAG AGGCCGTGCC 
CCTCAGGTAC TGGTTCTTGC ACCTACAAGA GAGTTGGCAA ATCAAGTAAG CAAAGACTTC 
AGTGACATCA CAAAAAAGCT GTCAGTGGCT TGTTTTTATG GTGGAACTCC CTATGGAGGT 
CAATTTGAAC GCATGAGGAA TGGGATTGAT ATCCTGGTTG GAACACCAGG TCGTATCAAA 
GACCACATAC AGAATGGCAA ACTAGATCTC ACCAAACTTA AGCATGTTGT CCTGGATGAA 
GTGGACCAGA TGTTGGATAT GGGATTTGCT GATCAAGTGG AAGAGATTTT AAGTGTGGCA 
TACAAGAAAG ATTCTGAAGA CAATCCCCAA ACATTGCTTT TTTCTGCAAC TTGCCCTCAT 
TGGGTATTTA ATGTTGCCAA GAAATACATG AAATCTACAT ATGAACAGGT GGACCTGATT 
GGTAAAAAGA CTCAGAAAAC GGCAATAACT GTGGAGCATC TGGCTATTAA GTGCCACTGG 
ACTCAGAGGG CAGCAGTTAT TGGGGATGTC ATCCGAGTAT ATAGTGGTCA TCAAGGACGC 
ACTATCATCT TTTGTGAAAC CAAGAAAGAA GCCCAGGAGC TGTCCCAGAA TTCAGCTATA 
AAGCAGGATG CTCAGTCCTT GCATGGAGAC ATTCCACAGA AGCAAAGGGA AATCACCCTG 
AAAGGTTTTA GAAATGGTAG TTTTGGAGTT TTGGTGGCAA CCAATGTTGC TGCACGTGGG 
TTAGACATCC CTGAGGTTGA TTTGGTTATA CAAAGCTCTC CACCAAAGGA TGTAGAGTCC 
TACATTCATC GATCCGGGCG GACAGGCAGA GCTGGAAGGA CGGGGGTGTG CATCTGCTTT 
TATCAGCACA AGGAAGAATA TCAGTTAGTA CAAGTGGAGC AAAAAGCGGG AATTAAGTTC 
AAACGAATAG GTGTTCCTTC TGCAACAGAA ATAATAAAAG CTTCCAGCAA AGATGCCATC 
AGGCTTTTGG ATTCCGTGCC TCCCACTGCC ATTAGTCACT TCAAACAATC AGCTGAGAAG 
CTGATAGAGG AGAAGGGAGC TGTGGAAGCT CTGGCAGCAG CACTGGCCCA TATTTCAGGT 
GCCACGTCCG TAGACCAGCG CTCCTTGATC AACTCAAATG TGGGTTTTGT GACCATGATC 
TTGCAGTGCT CAATTGAAAT GCCAAATATT AGTTATGCTT GGAAAGAACT TAAAGAGCAG 
CTGGGCGAGG AGATTGATTC CAAAGTGAAG GGAATGGTTT TTCTCAAAGG AAAGCTGGGT 
GTTTGCTTTG ATGTACCTAC CGCATCAGTA ACAGAAATAC AGGAGAAATG GCATGATTCA 
CGACGCTGGC AGCTCTCTGT GGCCACAGAG CAACCAGAAC TGGAAGGACC ACGGGAAGGA 
TATGGAGGCT TCAGGGGACA GCGGGAAGGC AGTCGAGGCT TCAGGGGACA GCGGGACGGA 
AACAGAAGAT TCAGAGGACA GCGGGAAGGC AGTAGAGGCC CGAGAGGACA GCGATCAGGA 
GGTGGCAACA AAAGTAACAG ATCCCAAAAC AAAGGCCAGA AGCGGAGTTT CAGTAAAGCA 
TTTGGTCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004719.2
CDS	57..2408
Translation	MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	NM_004728.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004728.4

ORF Insert Sequence:

ATGCCGGGAA AACTCCGTAG TGACGCTGGT TTGGAATCAG ACACCGCAAT GAAAAAAGGG 
GAGACACTGC GAAAGCAAAC CGAGGAGAAA GAGAAAAAAG AGAAGCCAAA ATCTGATAAG 
ACTGAAGAGA TAGCAGAAGA GGAAGAAACT GTTTTCCCCA AAGCTAAACA AGTTAAAAAG 
AAAGCAGAGC CTTCTGAAGT TGACATGAAT TCTCCTAAAT CCAAAAAGGC AAAAAAGAAA 
GAGGAGCCAT CTCAAAATGA CATTTCTCCT AAAACCAAAA GTTTGAGAAA GAAAAAGGAG 
CCCATTGAAA AGAAAGTGGT TTCTTCTAAA ACCAAAAAAG TGACAAAAAA TGAGGAGCCT 
TCTGAGGAAG AAATAGATGC TCCTAAGCCC AAGAAGATGA AGAAAGAAAA GGAAATGAAT 
GGAGAAACTA GAGAGAAAAG CCCCAAACTG AAGAATGGAT TTCCTCATCC TGAACCGGAC 
TGTAACCCCA GTGAAGCTGC CAGTGAAGAA AGTAACAGTG AGATAGAGCA GGAAATACCT 
GTGGAACAAA AAGAAGGCGC TTTCTCTAAT TTTCCCATAT CTGAAGAAAC TATTAAACTT 
CTCAAAGGCC GAGGAGTGAC CTTCCTATTT CCTATACAAG CAAAGACATT CCATCATGTT 
TACAGCGGGA AGGACTTAAT TGCACAGGCA CGGACAGGAA CTGGGAAGAC ATTCTCCTTT 
GCCATCCCTT TGATTGAGAA ACTTCATGGG GAACTGCAAG ACAGGAAGAG AGGCCGTGCC 
CCTCAGGTAC TGGTTCTTGC ACCTACAAGA GAGTTGGCAA ATCAAGTAAG CAAAGACTTC 
AGTGACATCA CAAAAAAGCT GTCAGTGGCT TGTTTTTATG GTGGAACTCC CTATGGAGGT 
CAATTTGAAC GCATGAGGAA TGGGATTGAT ATCCTGGTTG GAACACCAGG TCGTATCAAA 
GACCACATAC AGAATGGCAA ACTAGATCTC ACCAAACTTA AGCATGTTGT CCTGGATGAA 
GTGGACCAGA TGTTGGATAT GGGATTTGCT GATCAAGTGG AAGAGATTTT AAGTGTGGCA 
TACAAGAAAG ATTCTGAAGA CAATCCCCAA ACATTGCTTT TTTCTGCAAC TTGCCCTCAT 
TGGGTATTTA ATGTTGCCAA GAAATACATG AAATCTACAT ATGAACAGGT GGACCTGATT 
GGTAAAAAGA CTCAGAAAAC GGCAATAACT GTGGAGCATC TGGCTATTAA GTGCCACTGG 
ACTCAGAGGG CAGCAGTTAT TGGGGATGTC ATCCGAGTAT ATAGTGGTCA TCAAGGACGC 
ACTATCATCT TTTGTGAAAC CAAGAAAGAA GCCCAGGAGC TGTCCCAGAA TTCAGCTATA 
AAGCAGGATG CTCAGTCCTT GCATGGAGAC ATTCCACAGA AGCAAAGGGA AATCACCCTG 
AAAGGTTTTA GAAATGGTAG TTTTGGAGTT TTGGTGGCAA CCAATGTTGC TGCACGTGGG 
TTAGACATCC CTGAGGTTGA TTTGGTTATA CAAAGCTCTC CACCAAAGGA TGTAGAGTCC 
TACATTCATC GATCCGGGCG GACAGGCAGA GCTGGAAGGA CGGGGGTGTG CATCTGCTTT 
TATCAGCACA AGGAAGAATA TCAGTTAGTA CAAGTGGAGC AAAAAGCGGG AATTAAGTTC 
AAACGAATAG GTGTTCCTTC TGCAACAGAA ATAATAAAAG CTTCCAGCAA AGATGCCATC 
AGGCTTTTGG ATTCCGTGCC TCCCACTGCC ATTAGTCACT TCAAACAATC AGCTGAGAAG 
CTGATAGAGG AGAAGGGAGC TGTGGAAGCT CTGGCAGCAG CACTGGCCCA TATTTCAGGT 
GCCACGTCCG TAGACCAGCG CTCCTTGATC AACTCAAATG TGGGTTTTGT GACCATGATC 
TTGCAGTGCT CAATTGAAAT GCCAAATATT AGTTATGCTT GGAAAGAACT TAAAGAGCAG 
CTGGGCGAGG AGATTGATTC CAAAGTGAAG GGAATGGTTT TTCTCAAAGG AAAGCTGGGT 
GTTTGCTTTG ATGTACCTAC CGCATCAGTA ACAGAAATAC AGGAGAAATG GCATGATTCA 
CGACGCTGGC AGCTCTCTGT GGCCACAGAG CAACCAGAAC TGGAAGGACC ACGGGAAGGA 
TATGGAGGCT TCAGGGGACA GCGGGAAGGC AGTCGAGGCT TCAGGGGACA GCGGGACGGA 
AACAGAAGAT TCAGAGGACA GCGGGAAGGC AGTAGAGGCC CGAGAGGACA GCGATCAGGA 
GGTGGCAACA AAAGTAACAG ATCCCAAAAC AAAGGCCAGA AGCGGAGTTT CAGTAAAGCA 
TTTGGTCAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX21 ( XM_011540336.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011538638.1 Homo sapiens DExD-box helicase 21 (DDX21), transcript variant X1, mRNA.

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CloneID	OHu62586
Clone ID Related Accession (Same CDS sequence)	XM_011540336.1
Accession Version	XM_011540336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nucleolar RNA helicase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGAA AACTCCGTAG TGACGCTGGT TTGGAATCAG ACACCGCAAT GAAAAAAGGG 
GAGACACTGC GAAAGCAAAC CGAGGAGAAA GAGAAAAAAG AGAAGCCAAA ATCTGATAAG 
ACTGAAGAGA TAGCAGAAGA GGAAGAAACT GTTTTCCCCA AAGCTAAACA AGTTAAAAAG 
AAAGCAGAGC CTTCTGAAGT TGACATGAAT TCTCCTAAAT CCAAAAAGGC AAAAAAGAAA 
GAGGAGCCAT CTCAAAATGA CATTTCTCCT AAAACCAAAA GTTTGAGAAA GAAAAAGGAG 
CCCATTGAAA AGAAAGTGGT TTCTTCTAAA ACCAAAAAAG TGACAAAAAA TGAGGAGCCT 
TCTGAGGAAG AAATAGATGC TCCTAAGCCC AAGAAGATGA AGAAAGAAAA GGAAATGAAT 
GGAGAAACTA GAGAGAAAAG CCCCAAACTG AAGAATGGAT TTCCTCATCC TGAACCGGAC 
TGTAACCCCA GTGAAGCTGC CAGTGAAGAA AGTAACAGTG AGATAGAGCA GGAAATACCT 
GTGGAACAAA AAGAAGGCGC TTTCTCTAAT TTTCCCATAT CTGAAGAAAC TATTAAACTT 
CTCAAAGGCC GAGGAGTGAC CTTCCTATTT CCTATACAAG CAAAGACATT CCATCATGTT 
TACAGCGGGA AGGACTTAAT TGCACAGGCA CGGACAGGAA CTGGGAAGAC ATTCTCCTTT 
GCCATCCCTT TGATTGAGAA ACTTCATGGG GAACTGCAAG ACAGGAAGAG AGGCCGTGCC 
CCTCAGGTAC TGGTTCTTGC ACCTACAAGA GAGTTGGCAA ATCAAGTAAG CAAAGACTTC 
AGTGACATCA CAAAAAAGCT GTCAGTGGCT TGTTTTTATG GTGGAACTCC CTATGGAGGT 
CAATTTGAAC GCATGAGGAA TGGGATTGAT ATCCTGGTTG GAACACCAGG TCGTATCAAA 
GACCACATAC AGAATGGCAA ACTAGATCTC ACCAAACTTA AGCATGTTGT CCTGGATGAA 
GTGGACCAGA TGTTGGATAT GGGATTTGCT GATCAAGTGG AAGAGATTTT AAGTGTGGCA 
TACAAGAAAG ATTCTGAAGA CAATCCCCAA ACATTGCTTT TTTCTGCAAC TTGCCCTCAT 
TGGGTATTTA ATGTTGCCAA GAAATACATG AAATCTACAT ATGAACAGGT GGACCTGATT 
GGTAAAAAGA CTCAGAAAAC GGCAATAACT GTGGAGCATC TGGCTATTAA GTGCCACTGG 
ACTCAGAGGG CAGCAGTTAT TGGGGATGTC ATCCGAGTAT ATAGTGGTCA TCAAGGACGC 
ACTATCATCT TTTGTGAAAC CAAGAAAGAA GCCCAGGAGC TGTCCCAGAA TTCAGCTATA 
AAGCAGGATG TAGAGTCCTA CATTCATCGA TCCGGGCGGA CAGGCAGAGC TGGAAGGACG 
GGGGTGTGCA TCTGCTTTTA TCAGCACAAG GAAGAATATC AGTTAGTACA AGTGGAGCAA 
AAAGCGGGAA TTAAGTTCAA ACGAATAGGT GTTCCTTCTG CAACAGAAAT AATAAAAGCT 
TCCAGCAAAG ATGCCATCAG GCTTTTGGAT TCCGTGCCTC CCACTGCCAT TAGTCACTTC 
AAACAATCAG CTGAGAAGCT GATAGAGGAG AAGGGAGCTG TGGAAGCTCT GGCAGCAGCA 
CTGGCCCATA TTTCAGGTGC CACGTCCGTA GACCAGCGCT CCTTGATCAA CTCAAATGTG 
GGTTTTGTGA CCATGATCTT GCAGTGCTCA ATTGAAATGC CAAATATTAG TTATGCTTGG 
AAAGAACTTA AAGAGCAGCT GGGCGAGGAG ATTGATTCCA AAGTGAAGGG AATGGTTTTT 
CTCAAAGGAA AGCTGGGTGT TTGCTTTGAT GTACCTACCG CATCAGTAAC AGAAATACAG 
GAGAAATGGC ATGATTCACG ACGCTGGCAG CTCTCTGTGG CCACAGAGCA ACCAGAACTG 
GAAGGACCAC GGGAAGGATA TGGAGGCTTC AGGGGACAGC GGGAAGGCAG TCGAGGCTTC 
AGGGGACAGC GGGACGGAAA CAGAAGATTC AGAGGACAGC GGGAAGGCAG TAGAGGCCCG 
AGAGGACAGC GATCAGGAGG TGGCAACAAA AGTAACAGAT CCCAAAACAA AGGCCAGAAG 
CGGAGTTTCA GTAAAGCATT TGGTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011538638.1
CDS	96..2285
Translation	MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	XM_011540336.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540336.1

ORF Insert Sequence:

ATGCCGGGAA AACTCCGTAG TGACGCTGGT TTGGAATCAG ACACCGCAAT GAAAAAAGGG 
GAGACACTGC GAAAGCAAAC CGAGGAGAAA GAGAAAAAAG AGAAGCCAAA ATCTGATAAG 
ACTGAAGAGA TAGCAGAAGA GGAAGAAACT GTTTTCCCCA AAGCTAAACA AGTTAAAAAG 
AAAGCAGAGC CTTCTGAAGT TGACATGAAT TCTCCTAAAT CCAAAAAGGC AAAAAAGAAA 
GAGGAGCCAT CTCAAAATGA CATTTCTCCT AAAACCAAAA GTTTGAGAAA GAAAAAGGAG 
CCCATTGAAA AGAAAGTGGT TTCTTCTAAA ACCAAAAAAG TGACAAAAAA TGAGGAGCCT 
TCTGAGGAAG AAATAGATGC TCCTAAGCCC AAGAAGATGA AGAAAGAAAA GGAAATGAAT 
GGAGAAACTA GAGAGAAAAG CCCCAAACTG AAGAATGGAT TTCCTCATCC TGAACCGGAC 
TGTAACCCCA GTGAAGCTGC CAGTGAAGAA AGTAACAGTG AGATAGAGCA GGAAATACCT 
GTGGAACAAA AAGAAGGCGC TTTCTCTAAT TTTCCCATAT CTGAAGAAAC TATTAAACTT 
CTCAAAGGCC GAGGAGTGAC CTTCCTATTT CCTATACAAG CAAAGACATT CCATCATGTT 
TACAGCGGGA AGGACTTAAT TGCACAGGCA CGGACAGGAA CTGGGAAGAC ATTCTCCTTT 
GCCATCCCTT TGATTGAGAA ACTTCATGGG GAACTGCAAG ACAGGAAGAG AGGCCGTGCC 
CCTCAGGTAC TGGTTCTTGC ACCTACAAGA GAGTTGGCAA ATCAAGTAAG CAAAGACTTC 
AGTGACATCA CAAAAAAGCT GTCAGTGGCT TGTTTTTATG GTGGAACTCC CTATGGAGGT 
CAATTTGAAC GCATGAGGAA TGGGATTGAT ATCCTGGTTG GAACACCAGG TCGTATCAAA 
GACCACATAC AGAATGGCAA ACTAGATCTC ACCAAACTTA AGCATGTTGT CCTGGATGAA 
GTGGACCAGA TGTTGGATAT GGGATTTGCT GATCAAGTGG AAGAGATTTT AAGTGTGGCA 
TACAAGAAAG ATTCTGAAGA CAATCCCCAA ACATTGCTTT TTTCTGCAAC TTGCCCTCAT 
TGGGTATTTA ATGTTGCCAA GAAATACATG AAATCTACAT ATGAACAGGT GGACCTGATT 
GGTAAAAAGA CTCAGAAAAC GGCAATAACT GTGGAGCATC TGGCTATTAA GTGCCACTGG 
ACTCAGAGGG CAGCAGTTAT TGGGGATGTC ATCCGAGTAT ATAGTGGTCA TCAAGGACGC 
ACTATCATCT TTTGTGAAAC CAAGAAAGAA GCCCAGGAGC TGTCCCAGAA TTCAGCTATA 
AAGCAGGATG TAGAGTCCTA CATTCATCGA TCCGGGCGGA CAGGCAGAGC TGGAAGGACG 
GGGGTGTGCA TCTGCTTTTA TCAGCACAAG GAAGAATATC AGTTAGTACA AGTGGAGCAA 
AAAGCGGGAA TTAAGTTCAA ACGAATAGGT GTTCCTTCTG CAACAGAAAT AATAAAAGCT 
TCCAGCAAAG ATGCCATCAG GCTTTTGGAT TCCGTGCCTC CCACTGCCAT TAGTCACTTC 
AAACAATCAG CTGAGAAGCT GATAGAGGAG AAGGGAGCTG TGGAAGCTCT GGCAGCAGCA 
CTGGCCCATA TTTCAGGTGC CACGTCCGTA GACCAGCGCT CCTTGATCAA CTCAAATGTG 
GGTTTTGTGA CCATGATCTT GCAGTGCTCA ATTGAAATGC CAAATATTAG TTATGCTTGG 
AAAGAACTTA AAGAGCAGCT GGGCGAGGAG ATTGATTCCA AAGTGAAGGG AATGGTTTTT 
CTCAAAGGAA AGCTGGGTGT TTGCTTTGAT GTACCTACCG CATCAGTAAC AGAAATACAG 
GAGAAATGGC ATGATTCACG ACGCTGGCAG CTCTCTGTGG CCACAGAGCA ACCAGAACTG 
GAAGGACCAC GGGAAGGATA TGGAGGCTTC AGGGGACAGC GGGAAGGCAG TCGAGGCTTC 
AGGGGACAGC GGGACGGAAA CAGAAGATTC AGAGGACAGC GGGAAGGCAG TAGAGGCCCG 
AGAGGACAGC GATCAGGAGG TGGCAACAAA AGTAACAGAT CCCAAAACAA AGGCCAGAAG 
CGGAGTTTCA GTAAAGCATT TGGTCAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX21 ( XM_017016910.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872399.1 Homo sapiens DExD-box helicase 21 (DDX21), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu91810
Clone ID Related Accession (Same CDS sequence)	XM_017016910.2 , XM_017016910.1
Accession Version	XM_017016910.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nucleolar RNA helicase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017016910.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CTAAATCCAA AAAGGCAAAA AAGAAAGAGG AGCCATCTCA AAATGACATT 
TCTCCTAAAA CCAAAAGTTT GAGAAAGAAA AAGGAGCCCA TTGAAAAGAA AGTGGTTTCT 
TCTAAAACCA AAAAAGTGAC AAAAAATGAG GAGCCTTCTG AGGAAGAAAT AGATGCTCCT 
AAGCCCAAGA AGATGAAGAA AGAAAAGGAA ATGAATGGAG AAACTAGAGA GAAAAGCCCC 
AAACTGAAGA ATGGATTTCC TCATCCTGAA CCGGACTGTA ACCCCAGTGA AGCTGCCAGT 
GAAGAAAGTA ACAGTGAGAT AGAGCAGGAA ATACCTGTGG AACAAAAAGA AGGCGCTTTC 
TCTAATTTTC CCATATCTGA AGAAACTATT AAACTTCTCA AAGGCCGAGG AGTGACCTTC 
CTATTTCCTA TACAAGCAAA GACATTCCAT CATGTTTACA GCGGGAAGGA CTTAATTGCA 
CAGGCACGGA CAGGAACTGG GAAGACATTC TCCTTTGCCA TCCCTTTGAT TGAGAAACTT 
CATGGGGAAC TGCAAGACAG GAAGAGAGGC CGTGCCCCTC AGGTACTGGT TCTTGCACCT 
ACAAGAGAGT TGGCAAATCA AGTAAGCAAA GACTTCAGTG ACATCACAAA AAAGCTGTCA 
GTGGCTTGTT TTTATGGTGG AACTCCCTAT GGAGGTCAAT TTGAACGCAT GAGGAATGGG 
ATTGATATCC TGGTTGGAAC ACCAGGTCGT ATCAAAGACC ACATACAGAA TGGCAAACTA 
GATCTCACCA AACTTAAGCA TGTTGTCCTG GATGAAGTGG ACCAGATGTT GGATATGGGA 
TTTGCTGATC AAGTGGAAGA GATTTTAAGT GTGGCATACA AGAAAGATTC TGAAGACAAT 
CCCCAAACAT TGCTTTTTTC TGCAACTTGC CCTCATTGGG TATTTAATGT TGCCAAGAAA 
TACATGAAAT CTACATATGA ACAGGTGGAC CTGATTGGTA AAAAGACTCA GAAAACGGCA 
ATAACTGTGG AGCATCTGGC TATTAAGTGC CACTGGACTC AGAGGGCAGC AGTTATTGGG 
GATGTCATCC GAGTATATAG TGGTCATCAA GGACGCACTA TCATCTTTTG TGAAACCAAG 
AAAGAAGCCC AGGAGCTGTC CCAGAATTCA GCTATAAAGC AGGATGTAGA GTCCTACATT 
CATCGATCCG GGCGGACAGG CAGAGCTGGA AGGACGGGGG TGTGCATCTG CTTTTATCAG 
CACAAGGAAG AATATCAGTT AGTACAAGTG GAGCAAAAAG CGGGAATTAA GTTCAAACGA 
ATAGGTGTTC CTTCTGCAAC AGAAATAATA AAAGCTTCCA GCAAAGATGC CATCAGGCTT 
TTGGATTCCG TGCCTCCCAC TGCCATTAGT CACTTCAAAC AATCAGCTGA GAAGCTGATA 
GAGGAGAAGG GAGCTGTGGA AGCTCTGGCA GCAGCACTGG CCCATATTTC AGGTGCCACG 
TCCGTAGACC AGCGCTCCTT GATCAACTCA AATGTGGGTT TTGTGACCAT GATCTTGCAG 
TGCTCAATTG AAATGCCAAA TATTAGTTAT GCTTGGAAAG AACTTAAAGA GCAGCTGGGC 
GAGGAGATTG ATTCCAAAGT GAAGGGAATG GTTTTTCTCA AAGGAAAGCT GGGTGTTTGC 
TTTGATGTAC CTACCGCATC AGTAACAGAA ATACAGGAGA AATGGCATGA TTCACGACGC 
TGGCAGCTCT CTGTGGCCAC AGAGCAACCA GAACTGGAAG GACCACGGGA AGGATATGGA 
GGCTTCAGGG GACAGCGGGA AGGCAGTCGA GGCTTCAGGG GACAGCGGGA CGGAAACAGA 
AGATTCAGAG GACAGCGGGA AGGCAGTAGA GGCCCGAGAG GACAGCGATC AGGAGGTGGC 
AACAAAAGTA ACAGATCCCA AAACAAAGGC CAGAAGCGGA GTTTCAGTAA AGCATTTGGT 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872399.1
CDS	220..2205
Translation	MNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	XM_017016910.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DExD-box helicase 21 (DDX21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016910.2

ORF Insert Sequence:

ATGAATTCTC CTAAATCCAA AAAGGCAAAA AAGAAAGAGG AGCCATCTCA AAATGACATT 
TCTCCTAAAA CCAAAAGTTT GAGAAAGAAA AAGGAGCCCA TTGAAAAGAA AGTGGTTTCT 
TCTAAAACCA AAAAAGTGAC AAAAAATGAG GAGCCTTCTG AGGAAGAAAT AGATGCTCCT 
AAGCCCAAGA AGATGAAGAA AGAAAAGGAA ATGAATGGAG AAACTAGAGA GAAAAGCCCC 
AAACTGAAGA ATGGATTTCC TCATCCTGAA CCGGACTGTA ACCCCAGTGA AGCTGCCAGT 
GAAGAAAGTA ACAGTGAGAT AGAGCAGGAA ATACCTGTGG AACAAAAAGA AGGCGCTTTC 
TCTAATTTTC CCATATCTGA AGAAACTATT AAACTTCTCA AAGGCCGAGG AGTGACCTTC 
CTATTTCCTA TACAAGCAAA GACATTCCAT CATGTTTACA GCGGGAAGGA CTTAATTGCA 
CAGGCACGGA CAGGAACTGG GAAGACATTC TCCTTTGCCA TCCCTTTGAT TGAGAAACTT 
CATGGGGAAC TGCAAGACAG GAAGAGAGGC CGTGCCCCTC AGGTACTGGT TCTTGCACCT 
ACAAGAGAGT TGGCAAATCA AGTAAGCAAA GACTTCAGTG ACATCACAAA AAAGCTGTCA 
GTGGCTTGTT TTTATGGTGG AACTCCCTAT GGAGGTCAAT TTGAACGCAT GAGGAATGGG 
ATTGATATCC TGGTTGGAAC ACCAGGTCGT ATCAAAGACC ACATACAGAA TGGCAAACTA 
GATCTCACCA AACTTAAGCA TGTTGTCCTG GATGAAGTGG ACCAGATGTT GGATATGGGA 
TTTGCTGATC AAGTGGAAGA GATTTTAAGT GTGGCATACA AGAAAGATTC TGAAGACAAT 
CCCCAAACAT TGCTTTTTTC TGCAACTTGC CCTCATTGGG TATTTAATGT TGCCAAGAAA 
TACATGAAAT CTACATATGA ACAGGTGGAC CTGATTGGTA AAAAGACTCA GAAAACGGCA 
ATAACTGTGG AGCATCTGGC TATTAAGTGC CACTGGACTC AGAGGGCAGC AGTTATTGGG 
GATGTCATCC GAGTATATAG TGGTCATCAA GGACGCACTA TCATCTTTTG TGAAACCAAG 
AAAGAAGCCC AGGAGCTGTC CCAGAATTCA GCTATAAAGC AGGATGTAGA GTCCTACATT 
CATCGATCCG GGCGGACAGG CAGAGCTGGA AGGACGGGGG TGTGCATCTG CTTTTATCAG 
CACAAGGAAG AATATCAGTT AGTACAAGTG GAGCAAAAAG CGGGAATTAA GTTCAAACGA 
ATAGGTGTTC CTTCTGCAAC AGAAATAATA AAAGCTTCCA GCAAAGATGC CATCAGGCTT 
TTGGATTCCG TGCCTCCCAC TGCCATTAGT CACTTCAAAC AATCAGCTGA GAAGCTGATA 
GAGGAGAAGG GAGCTGTGGA AGCTCTGGCA GCAGCACTGG CCCATATTTC AGGTGCCACG 
TCCGTAGACC AGCGCTCCTT GATCAACTCA AATGTGGGTT TTGTGACCAT GATCTTGCAG 
TGCTCAATTG AAATGCCAAA TATTAGTTAT GCTTGGAAAG AACTTAAAGA GCAGCTGGGC 
GAGGAGATTG ATTCCAAAGT GAAGGGAATG GTTTTTCTCA AAGGAAAGCT GGGTGTTTGC 
TTTGATGTAC CTACCGCATC AGTAACAGAA ATACAGGAGA AATGGCATGA TTCACGACGC 
TGGCAGCTCT CTGTGGCCAC AGAGCAACCA GAACTGGAAG GACCACGGGA AGGATATGGA 
GGCTTCAGGG GACAGCGGGA AGGCAGTCGA GGCTTCAGGG GACAGCGGGA CGGAAACAGA 
AGATTCAGAG GACAGCGGGA AGGCAGTAGA GGCCCGAGAG GACAGCGATC AGGAGGTGGC 
AACAAAAGTA ACAGATCCCA AAACAAAGGC CAGAAGCGGA GTTTCAGTAA AGCATTTGGT 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX21 ( XM_017016910.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872399.1 Homo sapiens DEAD-box helicase 21 (DDX21), transcript variant X2, mRNA.

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CloneID	OHu91810
Clone ID Related Accession (Same CDS sequence)	XM_017016910.2 , XM_017016910.1
Accession Version	XM_017016910.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1986bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	nucleolar RNA helicase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CTAAATCCAA AAAGGCAAAA AAGAAAGAGG AGCCATCTCA AAATGACATT 
TCTCCTAAAA CCAAAAGTTT GAGAAAGAAA AAGGAGCCCA TTGAAAAGAA AGTGGTTTCT 
TCTAAAACCA AAAAAGTGAC AAAAAATGAG GAGCCTTCTG AGGAAGAAAT AGATGCTCCT 
AAGCCCAAGA AGATGAAGAA AGAAAAGGAA ATGAATGGAG AAACTAGAGA GAAAAGCCCC 
AAACTGAAGA ATGGATTTCC TCATCCTGAA CCGGACTGTA ACCCCAGTGA AGCTGCCAGT 
GAAGAAAGTA ACAGTGAGAT AGAGCAGGAA ATACCTGTGG AACAAAAAGA AGGCGCTTTC 
TCTAATTTTC CCATATCTGA AGAAACTATT AAACTTCTCA AAGGCCGAGG AGTGACCTTC 
CTATTTCCTA TACAAGCAAA GACATTCCAT CATGTTTACA GCGGGAAGGA CTTAATTGCA 
CAGGCACGGA CAGGAACTGG GAAGACATTC TCCTTTGCCA TCCCTTTGAT TGAGAAACTT 
CATGGGGAAC TGCAAGACAG GAAGAGAGGC CGTGCCCCTC AGGTACTGGT TCTTGCACCT 
ACAAGAGAGT TGGCAAATCA AGTAAGCAAA GACTTCAGTG ACATCACAAA AAAGCTGTCA 
GTGGCTTGTT TTTATGGTGG AACTCCCTAT GGAGGTCAAT TTGAACGCAT GAGGAATGGG 
ATTGATATCC TGGTTGGAAC ACCAGGTCGT ATCAAAGACC ACATACAGAA TGGCAAACTA 
GATCTCACCA AACTTAAGCA TGTTGTCCTG GATGAAGTGG ACCAGATGTT GGATATGGGA 
TTTGCTGATC AAGTGGAAGA GATTTTAAGT GTGGCATACA AGAAAGATTC TGAAGACAAT 
CCCCAAACAT TGCTTTTTTC TGCAACTTGC CCTCATTGGG TATTTAATGT TGCCAAGAAA 
TACATGAAAT CTACATATGA ACAGGTGGAC CTGATTGGTA AAAAGACTCA GAAAACGGCA 
ATAACTGTGG AGCATCTGGC TATTAAGTGC CACTGGACTC AGAGGGCAGC AGTTATTGGG 
GATGTCATCC GAGTATATAG TGGTCATCAA GGACGCACTA TCATCTTTTG TGAAACCAAG 
AAAGAAGCCC AGGAGCTGTC CCAGAATTCA GCTATAAAGC AGGATGTAGA GTCCTACATT 
CATCGATCCG GGCGGACAGG CAGAGCTGGA AGGACGGGGG TGTGCATCTG CTTTTATCAG 
CACAAGGAAG AATATCAGTT AGTACAAGTG GAGCAAAAAG CGGGAATTAA GTTCAAACGA 
ATAGGTGTTC CTTCTGCAAC AGAAATAATA AAAGCTTCCA GCAAAGATGC CATCAGGCTT 
TTGGATTCCG TGCCTCCCAC TGCCATTAGT CACTTCAAAC AATCAGCTGA GAAGCTGATA 
GAGGAGAAGG GAGCTGTGGA AGCTCTGGCA GCAGCACTGG CCCATATTTC AGGTGCCACG 
TCCGTAGACC AGCGCTCCTT GATCAACTCA AATGTGGGTT TTGTGACCAT GATCTTGCAG 
TGCTCAATTG AAATGCCAAA TATTAGTTAT GCTTGGAAAG AACTTAAAGA GCAGCTGGGC 
GAGGAGATTG ATTCCAAAGT GAAGGGAATG GTTTTTCTCA AAGGAAAGCT GGGTGTTTGC 
TTTGATGTAC CTACCGCATC AGTAACAGAA ATACAGGAGA AATGGCATGA TTCACGACGC 
TGGCAGCTCT CTGTGGCCAC AGAGCAACCA GAACTGGAAG GACCACGGGA AGGATATGGA 
GGCTTCAGGG GACAGCGGGA AGGCAGTCGA GGCTTCAGGG GACAGCGGGA CGGAAACAGA 
AGATTCAGAG GACAGCGGGA AGGCAGTAGA GGCCCGAGAG GACAGCGATC AGGAGGTGGC 
AACAAAAGTA ACAGATCCCA AAACAAAGGC CAGAAGCGGA GTTTCAGTAA AGCATTTGGT 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872399.1
CDS	218..2203
Translation	MNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ

Target ORF information:

RefSeq Version	XM_017016910.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DEAD-box helicase 21 (DDX21), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017016910.1

ORF Insert Sequence:

ATGAATTCTC CTAAATCCAA AAAGGCAAAA AAGAAAGAGG AGCCATCTCA AAATGACATT 
TCTCCTAAAA CCAAAAGTTT GAGAAAGAAA AAGGAGCCCA TTGAAAAGAA AGTGGTTTCT 
TCTAAAACCA AAAAAGTGAC AAAAAATGAG GAGCCTTCTG AGGAAGAAAT AGATGCTCCT 
AAGCCCAAGA AGATGAAGAA AGAAAAGGAA ATGAATGGAG AAACTAGAGA GAAAAGCCCC 
AAACTGAAGA ATGGATTTCC TCATCCTGAA CCGGACTGTA ACCCCAGTGA AGCTGCCAGT 
GAAGAAAGTA ACAGTGAGAT AGAGCAGGAA ATACCTGTGG AACAAAAAGA AGGCGCTTTC 
TCTAATTTTC CCATATCTGA AGAAACTATT AAACTTCTCA AAGGCCGAGG AGTGACCTTC 
CTATTTCCTA TACAAGCAAA GACATTCCAT CATGTTTACA GCGGGAAGGA CTTAATTGCA 
CAGGCACGGA CAGGAACTGG GAAGACATTC TCCTTTGCCA TCCCTTTGAT TGAGAAACTT 
CATGGGGAAC TGCAAGACAG GAAGAGAGGC CGTGCCCCTC AGGTACTGGT TCTTGCACCT 
ACAAGAGAGT TGGCAAATCA AGTAAGCAAA GACTTCAGTG ACATCACAAA AAAGCTGTCA 
GTGGCTTGTT TTTATGGTGG AACTCCCTAT GGAGGTCAAT TTGAACGCAT GAGGAATGGG 
ATTGATATCC TGGTTGGAAC ACCAGGTCGT ATCAAAGACC ACATACAGAA TGGCAAACTA 
GATCTCACCA AACTTAAGCA TGTTGTCCTG GATGAAGTGG ACCAGATGTT GGATATGGGA 
TTTGCTGATC AAGTGGAAGA GATTTTAAGT GTGGCATACA AGAAAGATTC TGAAGACAAT 
CCCCAAACAT TGCTTTTTTC TGCAACTTGC CCTCATTGGG TATTTAATGT TGCCAAGAAA 
TACATGAAAT CTACATATGA ACAGGTGGAC CTGATTGGTA AAAAGACTCA GAAAACGGCA 
ATAACTGTGG AGCATCTGGC TATTAAGTGC CACTGGACTC AGAGGGCAGC AGTTATTGGG 
GATGTCATCC GAGTATATAG TGGTCATCAA GGACGCACTA TCATCTTTTG TGAAACCAAG 
AAAGAAGCCC AGGAGCTGTC CCAGAATTCA GCTATAAAGC AGGATGTAGA GTCCTACATT 
CATCGATCCG GGCGGACAGG CAGAGCTGGA AGGACGGGGG TGTGCATCTG CTTTTATCAG 
CACAAGGAAG AATATCAGTT AGTACAAGTG GAGCAAAAAG CGGGAATTAA GTTCAAACGA 
ATAGGTGTTC CTTCTGCAAC AGAAATAATA AAAGCTTCCA GCAAAGATGC CATCAGGCTT 
TTGGATTCCG TGCCTCCCAC TGCCATTAGT CACTTCAAAC AATCAGCTGA GAAGCTGATA 
GAGGAGAAGG GAGCTGTGGA AGCTCTGGCA GCAGCACTGG CCCATATTTC AGGTGCCACG 
TCCGTAGACC AGCGCTCCTT GATCAACTCA AATGTGGGTT TTGTGACCAT GATCTTGCAG 
TGCTCAATTG AAATGCCAAA TATTAGTTAT GCTTGGAAAG AACTTAAAGA GCAGCTGGGC 
GAGGAGATTG ATTCCAAAGT GAAGGGAATG GTTTTTCTCA AAGGAAAGCT GGGTGTTTGC 
TTTGATGTAC CTACCGCATC AGTAACAGAA ATACAGGAGA AATGGCATGA TTCACGACGC 
TGGCAGCTCT CTGTGGCCAC AGAGCAACCA GAACTGGAAG GACCACGGGA AGGATATGGA 
GGCTTCAGGG GACAGCGGGA AGGCAGTCGA GGCTTCAGGG GACAGCGGGA CGGAAACAGA 
AGATTCAGAG GACAGCGGGA AGGCAGTAGA GGCCCGAGAG GACAGCGATC AGGAGGTGGC 
AACAAAAGTA ACAGATCCCA AAACAAAGGC CAGAAGCGGA GTTTCAGTAA AGCATTTGGT 
CAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.
Cell169(4)664-678.e16(2017 May)
Xing YH,Yao RW,Zhang Y,Guo CJ,Jiang S,Xu G,Dong R,Yang L,Chen LL

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DDX21 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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