EBAG9 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EBAG9
Entrez Gene ID	9166
Full Name	estrogen receptor binding site associated, antigen, 9
Synonyms	EB9,PDAF
General protein information	Preferred Names estrogen receptor binding site associated, antigen, 9 Names receptor-binding cancer antigen expressed on SiSo cells cancer-associated surface antigen RCAS1 estrogen receptor-binding fragment-associated gene 9 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q23.2
Summary	This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor, which binds to the estrogen-responsive element found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Alternate splicing results in multiple transcript variants. A pseudogene of this gene has been defined on chromosome 10. [provided by RefSeq, Jul 2013].
Disorder MIM:	605772

mRNA and Protein(s)

mRNA	Protein	Name
XM_017013960.1	XP_016869449.1	receptor-binding cancer antigen expressed on SiSo cells isoform X1
NM_001278938.1	NP_001265867.1	receptor-binding cancer antigen expressed on SiSo cells
NM_004215.4	NP_004206.1	receptor-binding cancer antigen expressed on SiSo cells
NM_004215.5	NP_004206.1	receptor-binding cancer antigen expressed on SiSo cells
NM_198120.2	NP_936056.1	receptor-binding cancer antigen expressed on SiSo cells

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ebag9	NP_001017088.1
	EBAG9	XP_002805507.1
Bos taurus (cattle)	EBAG9	NP_001071483.1
Homo sapiens (human)	EBAG9	NP_004206.1
Pan troglodytes (chimpanzee)	EBAG9	XP_519909.1
Canis lupus familiaris (dog)	EBAG9	NP_001002996.1
Mus musculus (house mouse)	Ebag9	NP_062353.3
Rattus norvegicus (Norway rat)	Ebag9	NP_001009665.1
Gallus gallus (chicken)	EBAG9	NP_001026120.2
Danio rerio (zebrafish)	ebag9	NP_957388.1

Gene Ontology
Bibliography

Related articles in PubMed

Diagnostic value of soluble receptor-binding cancer antigen expressed on SiSo cells and carcinoembryonic antigen in differentiating malignant from benign pleural effusion.
Dong J, Sun G, Zhu H
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(3)3257-64(2016 Mar)

Analysis of the intensity of immune cell infiltration and immunoreactivity of RCAS1 in diffuse large B-cell lymphoma of the palatine tonsil and its microenvironment.
Kazmierczak W, Lazar A, Tomaszewska R, Popiela TJ, Koper K, Wicherek L, Dutsch-Wicherek M
Cell and tissue research361(3)823-31(2015 Sep)

Expression of RCAS1 correlates with urothelial bladder cancer malignancy.
J??wicki W, Bro?yna AA, Siekiera J, Slominski AT
International journal of molecular sciences16(2)3783-803(2015 Feb)

Apoptotic function of tumor-associated antigen RCAS1 in oral squamous cell carcinoma.
Tanaka H, Toyoshima T, Sonoda K, Kitamura R, Sasaguri M, Kawano S, Matsubara R, Goto Y, Nakamura S
Journal of translational medicine12112(2014 May)

A disintegrin and metalloproteinase 9 is involved in ectodomain shedding of receptor-binding cancer antigen expressed on SiSo cells.
Sonoda K, Kato K
BioMed research international2014482396(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that joint detection of receptor-binding cancer antigen expressed on SiSo cells (RCAS1) and carcinoembryonic antigen (CEA) can improve the diagnostic sensitivity and specificity.
Title: Diagnostic value of soluble receptor-binding cancer antigen expressed on SiSo cells and carcinoembryonic antigen in differentiating malignant from benign pleural effusion.

RCAS1 seems to be involved in creating tumor-induced inflammation in the tumor and its microenvironment
Title: Analysis of the intensity of immune cell infiltration and immunoreactivity of RCAS1 in diffuse large B-cell lymphoma of the palatine tonsil and its microenvironment.

Findings suggest that the histological effect of increased RCAS1 expression depends on its cellular source and that RCAS1 expression itself is a component of various signaling pathways in urothelial bladder cancer cells.
Title: Expression of RCAS1 correlates with urothelial bladder cancer malignancy.

suggests that RCAS1 has an apoptotic function via membranous/soluble expression pattern in OSCC cells. RCAS1 may thus affect tumor escape from immune surveillance in OSCC by inducing apoptosis
Title: Apoptotic function of tumor-associated antigen RCAS1 in oral squamous cell carcinoma.

Given the significant correlation between tumor ADAM9 expression and serum RCAS1 concentration in both cervical and endometrial cancer as well as the role for ADAM9 in RCAS1 shedding.
Title: A disintegrin and metalloproteinase 9 is involved in ectodomain shedding of receptor-binding cancer antigen expressed on SiSo cells.

The following EBAG9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EBAG9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14197	NM_001278938.1 Latest version!	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14197	NM_198120.2 Latest version!	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14197	NM_004215.5 Latest version!	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14197	XM_017013960.1 Latest version!	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu14197	NM_004215.4	Homo sapiens estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EBAG9 ( NM_001278938.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001265867.1 Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu14197
Clone ID Related Accession (Same CDS sequence)	XM_017013960.1 , NM_001278938.1 , NM_004215.4 , NM_004215.5 , NM_198120.2
Accession Version	NM_001278938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	receptor-binding cancer antigen expressed on SiSo cells
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG616704.1, BC017729.1, AA812215.1, AI619447.1 and BC022506.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.295892.1, BG616704.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265867.1
CDS	116..757
Translation	MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Target ORF information:

RefSeq Version	NM_001278938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278938.1

ORF Insert Sequence:

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBAG9 ( NM_004215.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004206.1 Homo sapiens estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14197
Clone ID Related Accession (Same CDS sequence)	XM_017013960.1 , NM_001278938.1 , NM_004215.4 , NM_004215.5 , NM_198120.2
Accession Version	NM_004215.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	receptor-binding cancer antigen expressed on SiSo cells
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI194881.1, CD108096.1, BC022506.1, AA812215.1 and AI619447.1. On Jul 19, 2013 this sequence version replaced NM_004215.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022506.1, SRR1660809.202546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004206.1
CDS	403..1044
Translation	MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Target ORF information:

RefSeq Version	NM_004215.4
Organism	Homo sapiens(human)
Definition	Homo sapiens estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004215.4

ORF Insert Sequence:

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBAG9 ( NM_004215.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004206.1 Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14197
Clone ID Related Accession (Same CDS sequence)	XM_017013960.1 , NM_001278938.1 , NM_004215.4 , NM_004215.5 , NM_198120.2
Accession Version	NM_004215.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	receptor-binding cancer antigen expressed on SiSo cells
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD108096.1, BC022506.1, AA812215.1 and AI619447.1. On Nov 23, 2018 this sequence version replaced NM_004215.4. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC022506.1, SRR1660809.202546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000337573.10/ ENSP00000337675.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004206.1
CDS	305..946
Translation	MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Target ORF information:

RefSeq Version	NM_004215.5
Organism	Homo sapiens(human)
Definition	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004215.5

ORF Insert Sequence:

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBAG9 ( NM_198120.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_936056.1 Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14197
Clone ID Related Accession (Same CDS sequence)	XM_017013960.1 , NM_001278938.1 , NM_004215.4 , NM_004215.5 , NM_198120.2
Accession Version	NM_198120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	receptor-binding cancer antigen expressed on SiSo cells
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG707115.1, BC017729.1, AA812215.1, AI619447.1 and BC022506.1. On Jul 19, 2013 this sequence version replaced NM_198120.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.33459.1, AK290651.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_936056.1
CDS	236..877
Translation	MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Target ORF information:

RefSeq Version	NM_198120.2
Organism	Homo sapiens(human)
Definition	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198120.2

ORF Insert Sequence:

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EBAG9 ( XM_017013960.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016869449.1 Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant X1, mRNA.

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CloneID	OHu14197
Clone ID Related Accession (Same CDS sequence)	XM_017013960.1 , NM_001278938.1 , NM_004215.4 , NM_004215.5 , NM_198120.2
Accession Version	XM_017013960.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 642bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	receptor-binding cancer antigen expressed on SiSo cells isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000008.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016869449.1
CDS	599..1240
Translation	MAITQFRLFKFCTCLATVFSFLKRLICRSGRGRKLSGDQITLPTTVDYSSVPKQTDVEEWTSWDEDAPTSVKIEGGNGNVATQQNSLEQLEPDYFKDMTPTIRKTQKIVIKKREPLNFGIPDGSTGFSSRLAATQDLPFIHQSSELGDLDTWQENTNAWEEEEDAAWQAEEVLRQQKLADREKRAAEQQRKKMEKEAQRLMKKEQNKIGVKLS

Target ORF information:

RefSeq Version	XM_017013960.1
Organism	Homo sapiens(human)
Definition	Homo sapiens estrogen receptor binding site associated antigen 9 (EBAG9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017013960.1

ORF Insert Sequence:

ATGGCCATCA CCCAGTTTCG GTTATTTAAA TTTTGTACCT GCCTAGCAAC AGTATTCTCA 
TTCCTAAAGA GATTAATATG CAGATCTGGC AGAGGACGGA AATTAAGTGG AGACCAAATA 
ACTTTGCCAA CTACAGTTGA TTATTCATCA GTTCCTAAGC AGACAGATGT TGAAGAGTGG 
ACTTCCTGGG ATGAAGATGC ACCCACCAGT GTAAAGATCG AAGGAGGGAA TGGGAATGTG 
GCAACACAAC AAAATTCTTT GGAACAACTG GAACCTGACT ATTTTAAGGA CATGACACCA 
ACTATTAGGA AAACTCAGAA AATTGTTATT AAGAAGAGAG AACCATTGAA TTTTGGCATC 
CCAGATGGGA GCACAGGTTT CTCTAGTAGA TTAGCAGCTA CACAAGATCT GCCTTTTATT 
CATCAGTCTT CTGAATTAGG TGACTTAGAT ACCTGGCAGG AAAATACCAA TGCATGGGAA 
GAAGAAGAAG ATGCAGCCTG GCAAGCAGAA GAAGTTCTGA GACAGCAGAA ACTAGCAGAC 
AGAGAAAAGA GAGCAGCCGA ACAACAAAGG AAGAAAATGG AAAAGGAAGC ACAACGGCTA 
ATGAAGAAGG AACAAAACAA AATTGGTGTG AAACTTTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diagnostic value of soluble receptor-binding cancer antigen expressed on SiSo cells and carcinoembryonic antigen in differentiating malignant from benign pleural effusion.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine37(3)3257-64(2016 Mar)
Dong J,Sun G,Zhu H

Analysis of the intensity of immune cell infiltration and immunoreactivity of RCAS1 in diffuse large B-cell lymphoma of the palatine tonsil and its microenvironment.
Cell and tissue research361(3)823-31(2015 Sep)
Kazmierczak W,Lazar A,Tomaszewska R,Popiela TJ,Koper K,Wicherek L,Dutsch-Wicherek M

Expression of RCAS1 correlates with urothelial bladder cancer malignancy.
International journal of molecular sciences16(2)3783-803(2015 Feb)
J??wicki W,Bro?yna AA,Siekiera J,Slominski AT

Apoptotic function of tumor-associated antigen RCAS1 in oral squamous cell carcinoma.
Journal of translational medicine12112(2014 May)
Tanaka H,Toyoshima T,Sonoda K,Kitamura R,Sasaguri M,Kawano S,Matsubara R,Goto Y,Nakamura S

A disintegrin and metalloproteinase 9 is involved in ectodomain shedding of receptor-binding cancer antigen expressed on SiSo cells.
BioMed research international2014482396(2014)
Sonoda K,Kato K

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EBAG9 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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