AIFM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	AIFM1
Entrez Gene ID	9131
Full Name	apoptosis inducing factor mitochondria associated 1
Synonyms	AIF,CMT2D,CMTX4,COWCK,COXPD6,DFNX5,NADMR,NAMSD,PDCD8
General protein information	Preferred Names apoptosis inducing factor mitochondria associated 1 Names apoptosis-inducing factor 1, mitochondrial apoptosis-inducing factor, mitochondrion-associated, 1 programmed cell death 8 (apoptosis-inducing factor) striatal apoptosis-inducing factor testicular secretory protein Li 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq26.1
Summary	This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6 (COXPD6), a severe mitochondrial encephalomyopathy, as well as Cowchock syndrome, also known as X-linked recessive Charcot-Marie-Tooth disease-4 (CMTX-4), a disorder resulting in neuropathy, and axonal and motor-sensory defects with deafness and cognitive disability. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Aug 2015].
Disorder MIM:	300169
Disorder Html:	Combined oxidative phosphorylation deficiency 6, 300816 (3); Cowchock syndrome, 310490 (3); Deafness, X-linked 5, 300614 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004208.3	NP_004199.1	apoptosis-inducing factor 1, mitochondrial isoform AIF precursor
NM_001130847.3	NP_001124319.1	apoptosis-inducing factor 1, mitochondrial isoform AIFsh2 precursor
NM_001130846.2	NP_001124318.1	apoptosis-inducing factor 1, mitochondrial isoform 4
NM_145812.2	NP_665811.1	apoptosis-inducing factor 1, mitochondrial isoform AIF-exB precursor
NM_001130846.3	NP_001124318.2	apoptosis-inducing factor 1, mitochondrial isoform AIFsh
NM_004208.4	NP_004199.1	apoptosis-inducing factor 1, mitochondrial isoform AIF precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	AIFM1	NP_001179913.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008044	XP_317418.4
Caenorhabditis elegans (roundworm)	wah-1	NP_499564.2
Canis lupus familiaris (dog)	AIFM1	XP_538170.2
Mus musculus (house mouse)	Aifm1	NP_036149.1
Gallus gallus (chicken)	AIFM1	NP_001007491.1
Drosophila melanogaster (fruit fly)	AIF	NP_001259907.1
Rattus norvegicus (Norway rat)	Aifm1	NP_112646.1
Homo sapiens (human)	AIFM1	NP_004199.1
Danio rerio (zebrafish)	aifm1	NP_956396.2
Pan troglodytes (chimpanzee)	AIFM1	XP_003317723.1
	AIFM1	XP_001092025.1
Xenopus tropicalis (tropical clawed frog)	aifm1	NP_001017244.1

Gene Ontology
Bibliography

Related articles in PubMed

Influenza virus infection induces translocation of apoptosis-inducing factor (AIF) in A549 cells: role of AIF in apoptosis and viral propagation.
Qu X, Ding X, Duan M, Yang J, Lin R, Zhou Z, Wang S
Archives of virology162(3)669-675(2017 Mar)

AIF-independent parthanatos in the pathogenesis of dry age-related macular degeneration.
Jang KH, Do YJ, Son D, Son E, Choi JS, Kim E
Cell death & disease8(1)e2526(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

AIF translocation plays a role in influenza-virus-induced apoptosis.
Title: Influenza virus infection induces translocation of apoptosis-inducing factor (AIF) in A549 cells: role of AIF in apoptosis and viral propagation.

we report that AIF-independent PARP-1-dependent necrosis constitutes a major mechanism of RPE cell death leading to retinal degeneration in dry age-related macular degeneration .
Title: AIF-independent parthanatos in the pathogenesis of dry age-related macular degeneration.

Study defines the mechanism whereby AIF is allosterically modulated by charge-complex formation with NADH. Two distinct molecular pathways couple AIF's active site to dimerization and release of its regulatory C-loop insert with implications for AIF allosteric switching in cell survival and death.
Title: Defining NADH-Driven Allostery Regulating Apoptosis-Inducing Factor.

Downregulation of AIF by hypoxia causes oxidative inactivation of the lipid phosphatase activity of phosphatase and tensin homolog on chromosome 10 (PTEN), with ensuing activation of Akt kinase, phosphorylation of the Akt substrate GSK-3beta and activation of WNT/beta-catenin signaling in colon cancer cells.
Title: Downregulation of AIF by HIF-1 contributes to hypoxia-induced epithelial-mesenchymal transition of colon cancer.

AIF may participate in but not be dominant in the process of retinal pigment epithelium cell apoptosis induced by oxidative stress.
Title: The role of apoptosis inducing factor in the apoptosis of retinal pigment epithelium cells induced by oxidative stress.

The following AIFM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AIFM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26734	NM_001130846.2 Latest version!	Homo sapiens apoptosis-inducing factor, mitochondrion-associated, 1 (AIFM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
OHu27307	NM_004208.4 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu101512	NM_001130846.3 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu27083	NM_145812.2 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu27310	NM_001130847.3 Latest version!	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu27307	NM_004208.3	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AIFM1 ( NM_001130846.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124318.1 Homo sapiens apoptosis-inducing factor, mitochondrion-associated, 1 (AIFM1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu26734
Clone ID Related Accession (Same CDS sequence)	NM_001130846.2
Accession Version	NM_001130846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-05-12
Organism	Homo sapiens(Human)
Product	apoptosis-inducing factor 1, mitochondrial isoform 4
Comment

ATGGAAAAAG TCAGACGAGA GGGGGTTAAG GTGATGCCCA ATGCTATTGT GCAATCCGTT 
GGAGTCAGCA GTGGCAAGTT ACTTATCAAG CTGAAAGACG GCAGGAAGGT AGAAACTGAC 
CACATAGTGG CAGCTGTGGG CCTGGAGCCC AATGTTGAGT TGGCCAAGAC TGGTGGCCTG 
GAAATAGACT CAGATTTTGG TGGCTTCCGG GTAAATGCAG AGCTACAAGC ACGCTCTAAC 
ATCTGGGTGG CAGGAGATGC TGCATGCTTC TACGATATAA AGTTGGGAAG GAGGCGGGTA 
GAGCACCATG ATCACGCTGT TGTGAGTGGA AGATTGGCTG GAGAAAATAT GACTGGAGCT 
GCTAAGCCGT ACTGGCATCA GTCAATGTTC TGGAGTGATT TGGGCCCCGA TGTTGGCTAT 
GAAGCTATTG GTCTTGTGGA CAGTAGTTTG CCCACAGTTG GTGTTTTTGC AAAAGCAACT 
GCACAAGACA ACCCCAAATC TGCCACAGAG CAGTCAGGAA CTGGTATCCG ATCAGAGAGT 
GAGACAGAGT CCGAGGCCTC AGAAATTACT ATTCCTCCCA GCACCCCGGC AGTTCCACAG 
GCTCCCGTCC AGGGGGAGGA CTACGGCAAA GGTGTCATCT TCTACCTCAG GGACAAAGTG 
GTCGTGGGGA TTGTGCTATG GAACATCTTT AACCGAATGC CAATAGCAAG GAAGATCATT 
AAGGACGGTG AGCAGCATGA AGATCTCAAT GAAGTAGCCA AACTATTCAA CATTCATGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124318.1
Translation	MEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Target ORF information:

RefSeq Version	NM_001130846.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens apoptosis-inducing factor, mitochondrion-associated, 1 (AIFM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130846.2

ORF Insert Sequence:

ATGGAAAAAG TCAGACGAGA GGGGGTTAAG GTGATGCCCA ATGCTATTGT GCAATCCGTT 
GGAGTCAGCA GTGGCAAGTT ACTTATCAAG CTGAAAGACG GCAGGAAGGT AGAAACTGAC 
CACATAGTGG CAGCTGTGGG CCTGGAGCCC AATGTTGAGT TGGCCAAGAC TGGTGGCCTG 
GAAATAGACT CAGATTTTGG TGGCTTCCGG GTAAATGCAG AGCTACAAGC ACGCTCTAAC 
ATCTGGGTGG CAGGAGATGC TGCATGCTTC TACGATATAA AGTTGGGAAG GAGGCGGGTA 
GAGCACCATG ATCACGCTGT TGTGAGTGGA AGATTGGCTG GAGAAAATAT GACTGGAGCT 
GCTAAGCCGT ACTGGCATCA GTCAATGTTC TGGAGTGATT TGGGCCCCGA TGTTGGCTAT 
GAAGCTATTG GTCTTGTGGA CAGTAGTTTG CCCACAGTTG GTGTTTTTGC AAAAGCAACT 
GCACAAGACA ACCCCAAATC TGCCACAGAG CAGTCAGGAA CTGGTATCCG ATCAGAGAGT 
GAGACAGAGT CCGAGGCCTC AGAAATTACT ATTCCTCCCA GCACCCCGGC AGTTCCACAG 
GCTCCCGTCC AGGGGGAGGA CTACGGCAAA GGTGTCATCT TCTACCTCAG GGACAAAGTG 
GTCGTGGGGA TTGTGCTATG GAACATCTTT AACCGAATGC CAATAGCAAG GAAGATCATT 
AAGGACGGTG AGCAGCATGA AGATCTCAAT GAAGTAGCCA AACTATTCAA CATTCATGAA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM1 ( NM_004208.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004199.1 Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27307
Clone ID Related Accession (Same CDS sequence)	NM_004208.3 , NM_004208.4
Accession Version	NM_004208.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 1, mitochondrial isoform AIF precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA624842.1, BC139738.1 and BC111065.1. This sequence is a reference standard in the RefSeqGene project. On May 27, 2010 this sequence version replaced NM_004208.2. Transcript Variant: This variant (1) encodes the longest isoform (AIF). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK000775.1, SRR1660807.114105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGGCAA CTTGTTCCAG 
CGATGGCATG TTCCTCTAGA ACTCCAGATG ACAAGACAAA TGGCTAGCTC TGGTGCATCA 
GGGGGCAAAA TCGATAATTC TGTGTTAGTC CTTATTGTGG GCTTATCAAC AGTAGGAGCT 
GGTGCCTATG CCTACAAGAC TATGAAAGAG GATGAAAAAA GATACAATGA AAGAATTTCA 
GGGTTAGGGC TGACACCAGA ACAGAAACAG AAAAAGGCCG CGTTATCTGC TTCAGAAGGA 
GAGGAAGTTC CTCAAGACAA GGCGCCAAGT CATGTTCCTT TCCTGCTAAT TGGTGGAGGC 
ACAGCTGCTT TTGCTGCAGC CAGATCCATC CGGGCTCGGG ATCCTGGGGC CAGGGTACTG 
ATTGTATCTG AAGATCCTGA GCTGCCGTAC ATGCGACCTC CTCTTTCAAA AGAACTGTGG 
TTTTCAGATG ACCCAAATGT CACAAAGACA CTGCGATTCA AACAGTGGAA TGGAAAAGAG 
AGAAGCATAT ATTTCCAGCC ACCTTCTTTC TATGTCTCTG CTCAGGACCT GCCTCATATT 
GAGAATGGTG GTGTGGCTGT CCTCACTGGG AAGAAGGTAG TACAGCTGGA TGTGAGAGAC 
AACATGGTGA AACTTAATGA TGGCTCTCAA ATAACCTATG AAAAGTGCTT GATTGCAACA 
GGAGGTACTC CAAGAAGTCT GTCTGCCATT GATAGGGCTG GAGCAGAGGT GAAGAGTAGA 
ACAACGCTTT TCAGAAAGAT TGGAGACTTT AGAAGCTTGG AGAAGATTTC ACGGGAAGTC 
AAATCAATTA CGATTATCGG TGGGGGCTTC CTTGGTAGCG AACTGGCCTG TGCTCTTGGC 
AGAAAGGCTC GAGCCTTGGG CACAGAAGTG ATTCAACTCT TCCCCGAGAA AGGAAATATG 
GGAAAGATCC TCCCCGAATA CCTCAGCAAC TGGACCATGG AAAAAGTCAG ACGAGAGGGG 
GTTAAGGTGA TGCCCAATGC TATTGTGCAA TCCGTTGGAG TCAGCAGTGG CAAGTTACTT 
ATCAAGCTGA AAGACGGCAG GAAGGTAGAA ACTGACCACA TAGTGGCAGC TGTGGGCCTG 
GAGCCCAATG TTGAGTTGGC CAAGACTGGT GGCCTGGAAA TAGACTCAGA TTTTGGTGGC 
TTCCGGGTAA ATGCAGAGCT ACAAGCACGC TCTAACATCT GGGTGGCAGG AGATGCTGCA 
TGCTTCTACG ATATAAAGTT GGGAAGGAGG CGGGTAGAGC ACCATGATCA CGCTGTTGTG 
AGTGGAAGAT TGGCTGGAGA AAATATGACT GGAGCTGCTA AGCCGTACTG GCATCAGTCA 
ATGTTCTGGA GTGATTTGGG CCCCGATGTT GGCTATGAAG CTATTGGTCT TGTGGACAGT 
AGTTTGCCCA CAGTTGGTGT TTTTGCAAAA GCAACTGCAC AAGACAACCC CAAATCTGCC 
ACAGAGCAGT CAGGAACTGG TATCCGATCA GAGAGTGAGA CAGAGTCCGA GGCCTCAGAA 
ATTACTATTC CTCCCAGCAC CCCGGCAGTT CCACAGGCTC CCGTCCAGGG GGAGGACTAC 
GGCAAAGGTG TCATCTTCTA CCTCAGGGAC AAAGTGGTCG TGGGGATTGT GCTATGGAAC 
ATCTTTAACC GAATGCCAAT AGCAAGGAAG ATCATTAAGG ACGGTGAGCA GCATGAAGAT 
CTCAATGAAG TAGCCAAACT ATTCAACATT CATGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004199.1
CDS	232..2073
Translation	MFRCGGLAAGALKQKLVPLVRTVCVRSPRQRNRLPGNLFQRWHVPLELQMTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Target ORF information:

RefSeq Version	NM_004208.3
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004208.3

ORF Insert Sequence:

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGGCAA CTTGTTCCAG 
CGATGGCATG TTCCTCTAGA ACTCCAGATG ACAAGACAAA TGGCTAGCTC TGGTGCATCA 
GGGGGCAAAA TCGATAATTC TGTGTTAGTC CTTATTGTGG GCTTATCAAC AGTAGGAGCT 
GGTGCCTATG CCTACAAGAC TATGAAAGAG GATGAAAAAA GATACAATGA AAGAATTTCA 
GGGTTAGGGC TGACACCAGA ACAGAAACAG AAAAAGGCCG CGTTATCTGC TTCAGAAGGA 
GAGGAAGTTC CTCAAGACAA GGCGCCAAGT CATGTTCCTT TCCTGCTAAT TGGTGGAGGC 
ACAGCTGCTT TTGCTGCAGC CAGATCCATC CGGGCTCGGG ATCCTGGGGC CAGGGTACTG 
ATTGTATCTG AAGATCCTGA GCTGCCGTAC ATGCGACCTC CTCTTTCAAA AGAACTGTGG 
TTTTCAGATG ACCCAAATGT CACAAAGACA CTGCGATTCA AACAGTGGAA TGGAAAAGAG 
AGAAGCATAT ATTTCCAGCC ACCTTCTTTC TATGTCTCTG CTCAGGACCT GCCTCATATT 
GAGAATGGTG GTGTGGCTGT CCTCACTGGG AAGAAGGTAG TACAGCTGGA TGTGAGAGAC 
AACATGGTGA AACTTAATGA TGGCTCTCAA ATAACCTATG AAAAGTGCTT GATTGCAACA 
GGAGGTACTC CAAGAAGTCT GTCTGCCATT GATAGGGCTG GAGCAGAGGT GAAGAGTAGA 
ACAACGCTTT TCAGAAAGAT TGGAGACTTT AGAAGCTTGG AGAAGATTTC ACGGGAAGTC 
AAATCAATTA CGATTATCGG TGGGGGCTTC CTTGGTAGCG AACTGGCCTG TGCTCTTGGC 
AGAAAGGCTC GAGCCTTGGG CACAGAAGTG ATTCAACTCT TCCCCGAGAA AGGAAATATG 
GGAAAGATCC TCCCCGAATA CCTCAGCAAC TGGACCATGG AAAAAGTCAG ACGAGAGGGG 
GTTAAGGTGA TGCCCAATGC TATTGTGCAA TCCGTTGGAG TCAGCAGTGG CAAGTTACTT 
ATCAAGCTGA AAGACGGCAG GAAGGTAGAA ACTGACCACA TAGTGGCAGC TGTGGGCCTG 
GAGCCCAATG TTGAGTTGGC CAAGACTGGT GGCCTGGAAA TAGACTCAGA TTTTGGTGGC 
TTCCGGGTAA ATGCAGAGCT ACAAGCACGC TCTAACATCT GGGTGGCAGG AGATGCTGCA 
TGCTTCTACG ATATAAAGTT GGGAAGGAGG CGGGTAGAGC ACCATGATCA CGCTGTTGTG 
AGTGGAAGAT TGGCTGGAGA AAATATGACT GGAGCTGCTA AGCCGTACTG GCATCAGTCA 
ATGTTCTGGA GTGATTTGGG CCCCGATGTT GGCTATGAAG CTATTGGTCT TGTGGACAGT 
AGTTTGCCCA CAGTTGGTGT TTTTGCAAAA GCAACTGCAC AAGACAACCC CAAATCTGCC 
ACAGAGCAGT CAGGAACTGG TATCCGATCA GAGAGTGAGA CAGAGTCCGA GGCCTCAGAA 
ATTACTATTC CTCCCAGCAC CCCGGCAGTT CCACAGGCTC CCGTCCAGGG GGAGGACTAC 
GGCAAAGGTG TCATCTTCTA CCTCAGGGAC AAAGTGGTCG TGGGGATTGT GCTATGGAAC 
ATCTTTAACC GAATGCCAAT AGCAAGGAAG ATCATTAAGG ACGGTGAGCA GCATGAAGAT 
CTCAATGAAG TAGCCAAACT ATTCAACATT CATGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM1 ( NM_004208.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004199.1 Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27307
Clone ID Related Accession (Same CDS sequence)	NM_004208.3 , NM_004208.4
Accession Version	NM_004208.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 1, mitochondrial isoform AIF precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA624842.1, BC139738.1 and BC111065.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004208.3. Transcript Variant: This variant (1) encodes the longest isoform (AIF). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.65273.1, SRR1660807.114105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000287295.8/ ENSP00000287295.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGGCAA CTTGTTCCAG 
CGATGGCATG TTCCTCTAGA ACTCCAGATG ACAAGACAAA TGGCTAGCTC TGGTGCATCA 
GGGGGCAAAA TCGATAATTC TGTGTTAGTC CTTATTGTGG GCTTATCAAC AGTAGGAGCT 
GGTGCCTATG CCTACAAGAC TATGAAAGAG GATGAAAAAA GATACAATGA AAGAATTTCA 
GGGTTAGGGC TGACACCAGA ACAGAAACAG AAAAAGGCCG CGTTATCTGC TTCAGAAGGA 
GAGGAAGTTC CTCAAGACAA GGCGCCAAGT CATGTTCCTT TCCTGCTAAT TGGTGGAGGC 
ACAGCTGCTT TTGCTGCAGC CAGATCCATC CGGGCTCGGG ATCCTGGGGC CAGGGTACTG 
ATTGTATCTG AAGATCCTGA GCTGCCGTAC ATGCGACCTC CTCTTTCAAA AGAACTGTGG 
TTTTCAGATG ACCCAAATGT CACAAAGACA CTGCGATTCA AACAGTGGAA TGGAAAAGAG 
AGAAGCATAT ATTTCCAGCC ACCTTCTTTC TATGTCTCTG CTCAGGACCT GCCTCATATT 
GAGAATGGTG GTGTGGCTGT CCTCACTGGG AAGAAGGTAG TACAGCTGGA TGTGAGAGAC 
AACATGGTGA AACTTAATGA TGGCTCTCAA ATAACCTATG AAAAGTGCTT GATTGCAACA 
GGAGGTACTC CAAGAAGTCT GTCTGCCATT GATAGGGCTG GAGCAGAGGT GAAGAGTAGA 
ACAACGCTTT TCAGAAAGAT TGGAGACTTT AGAAGCTTGG AGAAGATTTC ACGGGAAGTC 
AAATCAATTA CGATTATCGG TGGGGGCTTC CTTGGTAGCG AACTGGCCTG TGCTCTTGGC 
AGAAAGGCTC GAGCCTTGGG CACAGAAGTG ATTCAACTCT TCCCCGAGAA AGGAAATATG 
GGAAAGATCC TCCCCGAATA CCTCAGCAAC TGGACCATGG AAAAAGTCAG ACGAGAGGGG 
GTTAAGGTGA TGCCCAATGC TATTGTGCAA TCCGTTGGAG TCAGCAGTGG CAAGTTACTT 
ATCAAGCTGA AAGACGGCAG GAAGGTAGAA ACTGACCACA TAGTGGCAGC TGTGGGCCTG 
GAGCCCAATG TTGAGTTGGC CAAGACTGGT GGCCTGGAAA TAGACTCAGA TTTTGGTGGC 
TTCCGGGTAA ATGCAGAGCT ACAAGCACGC TCTAACATCT GGGTGGCAGG AGATGCTGCA 
TGCTTCTACG ATATAAAGTT GGGAAGGAGG CGGGTAGAGC ACCATGATCA CGCTGTTGTG 
AGTGGAAGAT TGGCTGGAGA AAATATGACT GGAGCTGCTA AGCCGTACTG GCATCAGTCA 
ATGTTCTGGA GTGATTTGGG CCCCGATGTT GGCTATGAAG CTATTGGTCT TGTGGACAGT 
AGTTTGCCCA CAGTTGGTGT TTTTGCAAAA GCAACTGCAC AAGACAACCC CAAATCTGCC 
ACAGAGCAGT CAGGAACTGG TATCCGATCA GAGAGTGAGA CAGAGTCCGA GGCCTCAGAA 
ATTACTATTC CTCCCAGCAC CCCGGCAGTT CCACAGGCTC CCGTCCAGGG GGAGGACTAC 
GGCAAAGGTG TCATCTTCTA CCTCAGGGAC AAAGTGGTCG TGGGGATTGT GCTATGGAAC 
ATCTTTAACC GAATGCCAAT AGCAAGGAAG ATCATTAAGG ACGGTGAGCA GCATGAAGAT 
CTCAATGAAG TAGCCAAACT ATTCAACATT CATGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004199.1
CDS	186..2027
Translation	MFRCGGLAAGALKQKLVPLVRTVCVRSPRQRNRLPGNLFQRWHVPLELQMTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Target ORF information:

RefSeq Version	NM_004208.4
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004208.4

ORF Insert Sequence:

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGGCAA CTTGTTCCAG 
CGATGGCATG TTCCTCTAGA ACTCCAGATG ACAAGACAAA TGGCTAGCTC TGGTGCATCA 
GGGGGCAAAA TCGATAATTC TGTGTTAGTC CTTATTGTGG GCTTATCAAC AGTAGGAGCT 
GGTGCCTATG CCTACAAGAC TATGAAAGAG GATGAAAAAA GATACAATGA AAGAATTTCA 
GGGTTAGGGC TGACACCAGA ACAGAAACAG AAAAAGGCCG CGTTATCTGC TTCAGAAGGA 
GAGGAAGTTC CTCAAGACAA GGCGCCAAGT CATGTTCCTT TCCTGCTAAT TGGTGGAGGC 
ACAGCTGCTT TTGCTGCAGC CAGATCCATC CGGGCTCGGG ATCCTGGGGC CAGGGTACTG 
ATTGTATCTG AAGATCCTGA GCTGCCGTAC ATGCGACCTC CTCTTTCAAA AGAACTGTGG 
TTTTCAGATG ACCCAAATGT CACAAAGACA CTGCGATTCA AACAGTGGAA TGGAAAAGAG 
AGAAGCATAT ATTTCCAGCC ACCTTCTTTC TATGTCTCTG CTCAGGACCT GCCTCATATT 
GAGAATGGTG GTGTGGCTGT CCTCACTGGG AAGAAGGTAG TACAGCTGGA TGTGAGAGAC 
AACATGGTGA AACTTAATGA TGGCTCTCAA ATAACCTATG AAAAGTGCTT GATTGCAACA 
GGAGGTACTC CAAGAAGTCT GTCTGCCATT GATAGGGCTG GAGCAGAGGT GAAGAGTAGA 
ACAACGCTTT TCAGAAAGAT TGGAGACTTT AGAAGCTTGG AGAAGATTTC ACGGGAAGTC 
AAATCAATTA CGATTATCGG TGGGGGCTTC CTTGGTAGCG AACTGGCCTG TGCTCTTGGC 
AGAAAGGCTC GAGCCTTGGG CACAGAAGTG ATTCAACTCT TCCCCGAGAA AGGAAATATG 
GGAAAGATCC TCCCCGAATA CCTCAGCAAC TGGACCATGG AAAAAGTCAG ACGAGAGGGG 
GTTAAGGTGA TGCCCAATGC TATTGTGCAA TCCGTTGGAG TCAGCAGTGG CAAGTTACTT 
ATCAAGCTGA AAGACGGCAG GAAGGTAGAA ACTGACCACA TAGTGGCAGC TGTGGGCCTG 
GAGCCCAATG TTGAGTTGGC CAAGACTGGT GGCCTGGAAA TAGACTCAGA TTTTGGTGGC 
TTCCGGGTAA ATGCAGAGCT ACAAGCACGC TCTAACATCT GGGTGGCAGG AGATGCTGCA 
TGCTTCTACG ATATAAAGTT GGGAAGGAGG CGGGTAGAGC ACCATGATCA CGCTGTTGTG 
AGTGGAAGAT TGGCTGGAGA AAATATGACT GGAGCTGCTA AGCCGTACTG GCATCAGTCA 
ATGTTCTGGA GTGATTTGGG CCCCGATGTT GGCTATGAAG CTATTGGTCT TGTGGACAGT 
AGTTTGCCCA CAGTTGGTGT TTTTGCAAAA GCAACTGCAC AAGACAACCC CAAATCTGCC 
ACAGAGCAGT CAGGAACTGG TATCCGATCA GAGAGTGAGA CAGAGTCCGA GGCCTCAGAA 
ATTACTATTC CTCCCAGCAC CCCGGCAGTT CCACAGGCTC CCGTCCAGGG GGAGGACTAC 
GGCAAAGGTG TCATCTTCTA CCTCAGGGAC AAAGTGGTCG TGGGGATTGT GCTATGGAAC 
ATCTTTAACC GAATGCCAAT AGCAAGGAAG ATCATTAAGG ACGGTGAGCA GCATGAAGAT 
CTCAATGAAG TAGCCAAACT ATTCAACATT CATGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM1 ( NM_001130846.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124318.2 Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 4, mRNA.

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CloneID	OHu101512
Clone ID Related Accession (Same CDS sequence)	NM_001130846.3
Accession Version	NM_001130846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 825bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 1, mitochondrial isoform AIFsh
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ016496.2 and AI521711.1. On Aug 31, 2015 this sequence version replaced NM_001130846.2. Transcript Variant: This variant (4) lacks several 5' exons but contains an alternate 5' terminal exon, and it thus differs in the 5' UTR and initiates translation at a downstream in-frame start codon, compared to variant 1. The encoded isoform (AIFsh, also known as AIFshort) is shorter at the N-terminus, compared to isoform AIF. This variant can initiate translation at alternative start codons according to protein N-terminal sequencing and in vitro translation studies in PMID:16365034. The 5'-most start codon is represented in this RefSeq. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ016496.2, SRR1803613.183595.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154529, SAMEA2155550 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGAAAGA TCCTCCCCGA ATACCTCAGC AACTGGACCA TGGAAAAAGT CAGACGAGAG 
GGGGTTAAGG TGATGCCCAA TGCTATTGTG CAATCCGTTG GAGTCAGCAG TGGCAAGTTA 
CTTATCAAGC TGAAAGACGG CAGGAAGGTA GAAACTGACC ACATAGTGGC AGCTGTGGGC 
CTGGAGCCCA ATGTTGAGTT GGCCAAGACT GGTGGCCTGG AAATAGACTC AGATTTTGGT 
GGCTTCCGGG TAAATGCAGA GCTACAAGCA CGCTCTAACA TCTGGGTGGC AGGAGATGCT 
GCATGCTTCT ACGATATAAA GTTGGGAAGG AGGCGGGTAG AGCACCATGA TCACGCTGTT 
GTGAGTGGAA GATTGGCTGG AGAAAATATG ACTGGAGCTG CTAAGCCGTA CTGGCATCAG 
TCAATGTTCT GGAGTGATTT GGGCCCCGAT GTTGGCTATG AAGCTATTGG TCTTGTGGAC 
AGTAGTTTGC CCACAGTTGG TGTTTTTGCA AAAGCAACTG CACAAGACAA CCCCAAATCT 
GCCACAGAGC AGTCAGGAAC TGGTATCCGA TCAGAGAGTG AGACAGAGTC CGAGGCCTCA 
GAAATTACTA TTCCTCCCAG CACCCCGGCA GTTCCACAGG CTCCCGTCCA GGGGGAGGAC 
TACGGCAAAG GTGTCATCTT CTACCTCAGG GACAAAGTGG TCGTGGGGAT TGTGCTATGG 
AACATCTTTA ACCGAATGCC AATAGCAAGG AAGATCATTA AGGACGGTGA GCAGCATGAA 
GATCTCAATG AAGTAGCCAA ACTATTCAAC ATTCATGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124318.2
CDS	906..1730
Translation	MGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Target ORF information:

RefSeq Version	NM_001130846.3
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130846.3

ORF Insert Sequence:

ATGGGAAAGA TCCTCCCCGA ATACCTCAGC AACTGGACCA TGGAAAAAGT CAGACGAGAG 
GGGGTTAAGG TGATGCCCAA TGCTATTGTG CAATCCGTTG GAGTCAGCAG TGGCAAGTTA 
CTTATCAAGC TGAAAGACGG CAGGAAGGTA GAAACTGACC ACATAGTGGC AGCTGTGGGC 
CTGGAGCCCA ATGTTGAGTT GGCCAAGACT GGTGGCCTGG AAATAGACTC AGATTTTGGT 
GGCTTCCGGG TAAATGCAGA GCTACAAGCA CGCTCTAACA TCTGGGTGGC AGGAGATGCT 
GCATGCTTCT ACGATATAAA GTTGGGAAGG AGGCGGGTAG AGCACCATGA TCACGCTGTT 
GTGAGTGGAA GATTGGCTGG AGAAAATATG ACTGGAGCTG CTAAGCCGTA CTGGCATCAG 
TCAATGTTCT GGAGTGATTT GGGCCCCGAT GTTGGCTATG AAGCTATTGG TCTTGTGGAC 
AGTAGTTTGC CCACAGTTGG TGTTTTTGCA AAAGCAACTG CACAAGACAA CCCCAAATCT 
GCCACAGAGC AGTCAGGAAC TGGTATCCGA TCAGAGAGTG AGACAGAGTC CGAGGCCTCA 
GAAATTACTA TTCCTCCCAG CACCCCGGCA GTTCCACAGG CTCCCGTCCA GGGGGAGGAC 
TACGGCAAAG GTGTCATCTT CTACCTCAGG GACAAAGTGG TCGTGGGGAT TGTGCTATGG 
AACATCTTTA ACCGAATGCC AATAGCAAGG AAGATCATTA AGGACGGTGA GCAGCATGAA 
GATCTCAATG AAGTAGCCAA ACTATTCAAC ATTCATGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM1 ( NM_145812.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_665811.1 Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 2, mRNA.

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CloneID	OHu27083
Clone ID Related Accession (Same CDS sequence)	NM_145812.2
Accession Version	NM_145812.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 1, mitochondrial isoform AIF-exB precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA624842.1, AK314446.1 and BC111065.1. On May 27, 2010 this sequence version replaced NM_145812.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon but contains a different in-frame exon in the 5' coding region, compared to variant 1, resulting in an isoform (AIF-exB) that is shorter than isoform AIF. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111065.1, SRR1803617.218395.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGTTGT GCAGTCTCAT 
CACCTAGGAT CCCCTTCTAG ATCACTAGCA TCTACAGGTG CTTCTGGGAA AGATGGCAGC 
AACCTAGTGT ACTTCTTAAT TGTAGGAGCA ACAGTCACTG GGGCAGGAGT TTATTATGCC 
TACAAGACTA TGAAAGAGGA TGAAAAAAGA TACAATGAAA GAATTTCAGG GTTAGGGCTG 
ACACCAGAAC AGAAACAGAA AAAGGCCGCG TTATCTGCTT CAGAAGGAGA GGAAGTTCCT 
CAAGACAAGG CGCCAAGTCA TGTTCCTTTC CTGCTAATTG GTGGAGGCAC AGCTGCTTTT 
GCTGCAGCCA GATCCATCCG GGCTCGGGAT CCTGGGGCCA GGGTACTGAT TGTATCTGAA 
GATCCTGAGC TGCCGTACAT GCGACCTCCT CTTTCAAAAG AACTGTGGTT TTCAGATGAC 
CCAAATGTCA CAAAGACACT GCGATTCAAA CAGTGGAATG GAAAAGAGAG AAGCATATAT 
TTCCAGCCAC CTTCTTTCTA TGTCTCTGCT CAGGACCTGC CTCATATTGA GAATGGTGGT 
GTGGCTGTCC TCACTGGGAA GAAGGTAGTA CAGCTGGATG TGAGAGACAA CATGGTGAAA 
CTTAATGATG GCTCTCAAAT AACCTATGAA AAGTGCTTGA TTGCAACAGG AGGTACTCCA 
AGAAGTCTGT CTGCCATTGA TAGGGCTGGA GCAGAGGTGA AGAGTAGAAC AACGCTTTTC 
AGAAAGATTG GAGACTTTAG AAGCTTGGAG AAGATTTCAC GGGAAGTCAA ATCAATTACG 
ATTATCGGTG GGGGCTTCCT TGGTAGCGAA CTGGCCTGTG CTCTTGGCAG AAAGGCTCGA 
GCCTTGGGCA CAGAAGTGAT TCAACTCTTC CCCGAGAAAG GAAATATGGG AAAGATCCTC 
CCCGAATACC TCAGCAACTG GACCATGGAA AAAGTCAGAC GAGAGGGGGT TAAGGTGATG 
CCCAATGCTA TTGTGCAATC CGTTGGAGTC AGCAGTGGCA AGTTACTTAT CAAGCTGAAA 
GACGGCAGGA AGGTAGAAAC TGACCACATA GTGGCAGCTG TGGGCCTGGA GCCCAATGTT 
GAGTTGGCCA AGACTGGTGG CCTGGAAATA GACTCAGATT TTGGTGGCTT CCGGGTAAAT 
GCAGAGCTAC AAGCACGCTC TAACATCTGG GTGGCAGGAG ATGCTGCATG CTTCTACGAT 
ATAAAGTTGG GAAGGAGGCG GGTAGAGCAC CATGATCACG CTGTTGTGAG TGGAAGATTG 
GCTGGAGAAA ATATGACTGG AGCTGCTAAG CCGTACTGGC ATCAGTCAAT GTTCTGGAGT 
GATTTGGGCC CCGATGTTGG CTATGAAGCT ATTGGTCTTG TGGACAGTAG TTTGCCCACA 
GTTGGTGTTT TTGCAAAAGC AACTGCACAA GACAACCCCA AATCTGCCAC AGAGCAGTCA 
GGAACTGGTA TCCGATCAGA GAGTGAGACA GAGTCCGAGG CCTCAGAAAT TACTATTCCT 
CCCAGCACCC CGGCAGTTCC ACAGGCTCCC GTCCAGGGGG AGGACTACGG CAAAGGTGTC 
ATCTTCTACC TCAGGGACAA AGTGGTCGTG GGGATTGTGC TATGGAACAT CTTTAACCGA 
ATGCCAATAG CAAGGAAGAT CATTAAGGAC GGTGAGCAGC ATGAAGATCT CAATGAAGTA 
GCCAAACTAT TCAACATTCA TGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665811.1
CDS	232..2061
Translation	MFRCGGLAAGALKQKLVPLVRTVCVRSPRQRNRLPVVQSHHLGSPSRSLASTGASGKDGSNLVYFLIVGATVTGAGVYYAYKTMKEDEKRYNERISGLGLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKARALGTEVIQLFPEKGNMGKILPEYLSNWTMEKVRREGVKVMPNAIVQSVGVSSGKLLIKLKDGRKVETDHIVAAVGLEPNVELAKTGGLEIDSDFGGFRVNAELQARSNIWVAGDAACFYDIKLGRRRVEHHDHAVVSGRLAGENMTGAAKPYWHQSMFWSDLGPDVGYEAIGLVDSSLPTVGVFAKATAQDNPKSATEQSGTGIRSESETESEASEITIPPSTPAVPQAPVQGEDYGKGVIFYLRDKVVVGIVLWNIFNRMPIARKIIKDGEQHEDLNEVAKLFNIHED

Target ORF information:

RefSeq Version	NM_145812.2
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145812.2

ORF Insert Sequence:

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGTTGT GCAGTCTCAT 
CACCTAGGAT CCCCTTCTAG ATCACTAGCA TCTACAGGTG CTTCTGGGAA AGATGGCAGC 
AACCTAGTGT ACTTCTTAAT TGTAGGAGCA ACAGTCACTG GGGCAGGAGT TTATTATGCC 
TACAAGACTA TGAAAGAGGA TGAAAAAAGA TACAATGAAA GAATTTCAGG GTTAGGGCTG 
ACACCAGAAC AGAAACAGAA AAAGGCCGCG TTATCTGCTT CAGAAGGAGA GGAAGTTCCT 
CAAGACAAGG CGCCAAGTCA TGTTCCTTTC CTGCTAATTG GTGGAGGCAC AGCTGCTTTT 
GCTGCAGCCA GATCCATCCG GGCTCGGGAT CCTGGGGCCA GGGTACTGAT TGTATCTGAA 
GATCCTGAGC TGCCGTACAT GCGACCTCCT CTTTCAAAAG AACTGTGGTT TTCAGATGAC 
CCAAATGTCA CAAAGACACT GCGATTCAAA CAGTGGAATG GAAAAGAGAG AAGCATATAT 
TTCCAGCCAC CTTCTTTCTA TGTCTCTGCT CAGGACCTGC CTCATATTGA GAATGGTGGT 
GTGGCTGTCC TCACTGGGAA GAAGGTAGTA CAGCTGGATG TGAGAGACAA CATGGTGAAA 
CTTAATGATG GCTCTCAAAT AACCTATGAA AAGTGCTTGA TTGCAACAGG AGGTACTCCA 
AGAAGTCTGT CTGCCATTGA TAGGGCTGGA GCAGAGGTGA AGAGTAGAAC AACGCTTTTC 
AGAAAGATTG GAGACTTTAG AAGCTTGGAG AAGATTTCAC GGGAAGTCAA ATCAATTACG 
ATTATCGGTG GGGGCTTCCT TGGTAGCGAA CTGGCCTGTG CTCTTGGCAG AAAGGCTCGA 
GCCTTGGGCA CAGAAGTGAT TCAACTCTTC CCCGAGAAAG GAAATATGGG AAAGATCCTC 
CCCGAATACC TCAGCAACTG GACCATGGAA AAAGTCAGAC GAGAGGGGGT TAAGGTGATG 
CCCAATGCTA TTGTGCAATC CGTTGGAGTC AGCAGTGGCA AGTTACTTAT CAAGCTGAAA 
GACGGCAGGA AGGTAGAAAC TGACCACATA GTGGCAGCTG TGGGCCTGGA GCCCAATGTT 
GAGTTGGCCA AGACTGGTGG CCTGGAAATA GACTCAGATT TTGGTGGCTT CCGGGTAAAT 
GCAGAGCTAC AAGCACGCTC TAACATCTGG GTGGCAGGAG ATGCTGCATG CTTCTACGAT 
ATAAAGTTGG GAAGGAGGCG GGTAGAGCAC CATGATCACG CTGTTGTGAG TGGAAGATTG 
GCTGGAGAAA ATATGACTGG AGCTGCTAAG CCGTACTGGC ATCAGTCAAT GTTCTGGAGT 
GATTTGGGCC CCGATGTTGG CTATGAAGCT ATTGGTCTTG TGGACAGTAG TTTGCCCACA 
GTTGGTGTTT TTGCAAAAGC AACTGCACAA GACAACCCCA AATCTGCCAC AGAGCAGTCA 
GGAACTGGTA TCCGATCAGA GAGTGAGACA GAGTCCGAGG CCTCAGAAAT TACTATTCCT 
CCCAGCACCC CGGCAGTTCC ACAGGCTCCC GTCCAGGGGG AGGACTACGG CAAAGGTGTC 
ATCTTCTACC TCAGGGACAA AGTGGTCGTG GGGATTGTGC TATGGAACAT CTTTAACCGA 
ATGCCAATAG CAAGGAAGAT CATTAAGGAC GGTGAGCAGC ATGAAGATCT CAATGAAGTA 
GCCAAACTAT TCAACATTCA TGAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AIFM1 ( NM_001130847.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124319.1 Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 5, mRNA.

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CloneID	OHu27310
Clone ID Related Accession (Same CDS sequence)	NM_001130847.3
Accession Version	NM_001130847.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	apoptosis-inducing factor 1, mitochondrial isoform AIFsh2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA624842.1, DQ016498.1 and BC111065.1. On or before Aug 31, 2013 this sequence version replaced XR_244528.1, NM_001130847.2. Transcript Variant: This variant (5) contains an additional internal exon, which results in a distinct 3' coding region and differences in the 3' UTR, compared to variant 1. The encoded isoform (AIFsh2, also known as AIFshort2) has a distinct C-terminus and is shorter than isoform AIF. CCDS Note: This CCDS represents the AIFsh2 isoform described in PMID:16644725. The transcript is a candidate for nonsense-mediated mRNA decay (NMD), but the protein is represented based on evidence for the endogenous protein in PMID:16644725. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ016498.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta NMD candidate :: PMID: 16644725 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGGCAA CTTGTTCCAG 
CGATGGCATG TTCCTCTAGA ACTCCAGATG ACAAGACAAA TGGCTAGCTC TGGTGCATCA 
GGGGGCAAAA TCGATAATTC TGTGTTAGTC CTTATTGTGG GCTTATCAAC AGTAGGAGCT 
GGTGCCTATG CCTACAAGAC TATGAAAGAG GATGAAAAAA GATACAATGA AAGAATTTCA 
GGGTTAGGGC TGACACCAGA ACAGAAACAG AAAAAGGCCG CGTTATCTGC TTCAGAAGGA 
GAGGAAGTTC CTCAAGACAA GGCGCCAAGT CATGTTCCTT TCCTGCTAAT TGGTGGAGGC 
ACAGCTGCTT TTGCTGCAGC CAGATCCATC CGGGCTCGGG ATCCTGGGGC CAGGGTACTG 
ATTGTATCTG AAGATCCTGA GCTGCCGTAC ATGCGACCTC CTCTTTCAAA AGAACTGTGG 
TTTTCAGATG ACCCAAATGT CACAAAGACA CTGCGATTCA AACAGTGGAA TGGAAAAGAG 
AGAAGCATAT ATTTCCAGCC ACCTTCTTTC TATGTCTCTG CTCAGGACCT GCCTCATATT 
GAGAATGGTG GTGTGGCTGT CCTCACTGGG AAGAAGGTAG TACAGCTGGA TGTGAGAGAC 
AACATGGTGA AACTTAATGA TGGCTCTCAA ATAACCTATG AAAAGTGCTT GATTGCAACA 
GGAGGTACTC CAAGAAGTCT GTCTGCCATT GATAGGGCTG GAGCAGAGGT GAAGAGTAGA 
ACAACGCTTT TCAGAAAGAT TGGAGACTTT AGAAGCTTGG AGAAGATTTC ACGGGAAGTC 
AAATCAATTA CGATTATCGG TGGGGGCTTC CTTGGTAGCG AACTGGCCTG TGCTCTTGGC 
AGAAAGGATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124319.1
CDS	232..1206
Translation	MFRCGGLAAGALKQKLVPLVRTVCVRSPRQRNRLPGNLFQRWHVPLELQMTRQMASSGASGGKIDNSVLVLIVGLSTVGAGAYAYKTMKEDEKRYNERISGLGLTPEQKQKKAALSASEGEEVPQDKAPSHVPFLLIGGGTAAFAAARSIRARDPGARVLIVSEDPELPYMRPPLSKELWFSDDPNVTKTLRFKQWNGKERSIYFQPPSFYVSAQDLPHIENGGVAVLTGKKVVQLDVRDNMVKLNDGSQITYEKCLIATGGTPRSLSAIDRAGAEVKSRTTLFRKIGDFRSLEKISREVKSITIIGGGFLGSELACALGRKDI

Target ORF information:

RefSeq Version	NM_001130847.3
Organism	Homo sapiens(human)
Definition	Homo sapiens apoptosis inducing factor mitochondria associated 1 (AIFM1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130847.3

ORF Insert Sequence:

ATGTTCCGGT GTGGAGGCCT GGCGGCGGGT GCTTTGAAGC AGAAGCTGGT GCCCTTGGTG 
CGGACCGTGT GCGTCCGAAG CCCGAGGCAG AGGAACCGGC TCCCAGGCAA CTTGTTCCAG 
CGATGGCATG TTCCTCTAGA ACTCCAGATG ACAAGACAAA TGGCTAGCTC TGGTGCATCA 
GGGGGCAAAA TCGATAATTC TGTGTTAGTC CTTATTGTGG GCTTATCAAC AGTAGGAGCT 
GGTGCCTATG CCTACAAGAC TATGAAAGAG GATGAAAAAA GATACAATGA AAGAATTTCA 
GGGTTAGGGC TGACACCAGA ACAGAAACAG AAAAAGGCCG CGTTATCTGC TTCAGAAGGA 
GAGGAAGTTC CTCAAGACAA GGCGCCAAGT CATGTTCCTT TCCTGCTAAT TGGTGGAGGC 
ACAGCTGCTT TTGCTGCAGC CAGATCCATC CGGGCTCGGG ATCCTGGGGC CAGGGTACTG 
ATTGTATCTG AAGATCCTGA GCTGCCGTAC ATGCGACCTC CTCTTTCAAA AGAACTGTGG 
TTTTCAGATG ACCCAAATGT CACAAAGACA CTGCGATTCA AACAGTGGAA TGGAAAAGAG 
AGAAGCATAT ATTTCCAGCC ACCTTCTTTC TATGTCTCTG CTCAGGACCT GCCTCATATT 
GAGAATGGTG GTGTGGCTGT CCTCACTGGG AAGAAGGTAG TACAGCTGGA TGTGAGAGAC 
AACATGGTGA AACTTAATGA TGGCTCTCAA ATAACCTATG AAAAGTGCTT GATTGCAACA 
GGAGGTACTC CAAGAAGTCT GTCTGCCATT GATAGGGCTG GAGCAGAGGT GAAGAGTAGA 
ACAACGCTTT TCAGAAAGAT TGGAGACTTT AGAAGCTTGG AGAAGATTTC ACGGGAAGTC 
AAATCAATTA CGATTATCGG TGGGGGCTTC CTTGGTAGCG AACTGGCCTG TGCTCTTGGC 
AGAAAGGATA TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Influenza virus infection induces translocation of apoptosis-inducing factor (AIF) in A549 cells: role of AIF in apoptosis and viral propagation.
Archives of virology162(3)669-675(2017 Mar)
Qu X,Ding X,Duan M,Yang J,Lin R,Zhou Z,Wang S

AIF-independent parthanatos in the pathogenesis of dry age-related macular degeneration.
Cell death & disease8(1)e2526(2017 Jan)
Jang KH,Do YJ,Son D,Son E,Choi JS,Kim E

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AIFM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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