DNAJA3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DNAJA3
Entrez Gene ID	9093
Full Name	DnaJ heat shock protein family (Hsp40) member A3
Synonyms	HCA57,TID1,hTID-1
General protein information	Preferred Names DnaJ heat shock protein family (Hsp40) member A3 Names dnaJ homolog subfamily A member 3, mitochondrial DnaJ (Hsp40) homolog, subfamily A, member 3 dnaJ protein Tid-1 hepatocellular carcinoma-associated antigen 57 tumorous imaginal discs protein Tid56 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:	608382

mRNA and Protein(s)

mRNA	Protein	Name
NM_001135110.2	NP_001128582.1	dnaJ homolog subfamily A member 3, mitochondrial isoform 2
NM_005147.6	NP_005138.3	dnaJ homolog subfamily A member 3, mitochondrial isoform 1
NM_001286516.1	NP_001273445.1	dnaJ homolog subfamily A member 3, mitochondrial isoform 3
NM_005147.5	NP_005138.3	dnaJ homolog subfamily A member 3, mitochondrial isoform 1
NM_001135110.3	NP_001128582.1	dnaJ homolog subfamily A member 3, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DNAJA3	XP_510781.2
Rattus norvegicus (Norway rat)	Dnaja3	NP_001033684.1
Kluyveromyces lactis	KLLA0D07656g	XP_453404.1
Magnaporthe oryzae (rice blast fungus)	MGG_11144	XP_003714776.1
Neurospora crassa	NCU05196	XP_962195.1
Eremothecium gossypii	AGOS_AFR507W	NP_986054.1
Canis lupus familiaris (dog)	DNAJA3	XP_851751.1
Bos taurus (cattle)	DNAJA3	NP_001073739.1
Mus musculus (house mouse)	Dnaja3	NP_076135.3
Gallus gallus (chicken)	DNAJA3	XP_414967.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007565	XP_001687891.1
Schizosaccharomyces pombe (fission yeast)	mdj1	NP_587824.1
Oryza sativa (rice)	Os06g0116800	NP_001056623.1
Saccharomyces cerevisiae (baker's yeast)	MDJ1	NP_116638.1
Arabidopsis thaliana (thale cress)	GFA2	NP_568690.1
Oryza sativa (rice)	Os12g0168400	NP_001066254.1
Xenopus tropicalis (tropical clawed frog)	dnaja3	NP_001120377.1
Homo sapiens (human)	DNAJA3	NP_005138.3
Danio rerio (zebrafish)	dnaja3a	NP_958470.1
Drosophila melanogaster (fruit fly)	l(2)tid	NP_995932.1

Gene Ontology
Bibliography

Related articles in PubMed

The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-?B Signaling.
Momiuchi Y, Kumada K, Kuraishi T, Takagaki T, Aigaki T, Oshima Y, Kurata S
The Journal of biological chemistry290(39)23816-25(2015 Sep)

Tid1, the Mammalian Homologue of Drosophila Tumor Suppressor Tid56, Mediates Macroautophagy by Interacting with Beclin1-containing Autophagy Protein Complex.
Niu G, Zhang H, Liu D, Chen L, Belani C, Wang HG, Cheng H
The Journal of biological chemistry290(29)18102-10(2015 Jul)

Stoichiometric expression of mtHsp40 and mtHsp70 modulates mitochondrial morphology and cristae structure via Opa1L cleavage.
Lee B, Ahn Y, Kang SM, Park Y, Jeon YJ, Rho JM, Kim SW
Molecular biology of the cell26(12)2156-67(2015 Jun)

Tid1 is a new regulator of p53 mitochondrial translocation and apoptosis in cancer.
Ahn BY, Trinh DL, Zajchowski LD, Lee B, Elwi AN, Kim SW
Oncogene29(8)1155-66(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-kappaB Signaling.
Title: The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-?B Signaling.

Tid1 increases autophagic flux by interacting with the Beclin 1-containing autophagy protein complex.
Title: Tid1, the Mammalian Homologue of Drosophila Tumor Suppressor Tid56, Mediates Macroautophagy by Interacting with Beclin1-containing Autophagy Protein Complex.

Impaired stoichiometry between mtHsp40 and mtHsp70 promotes Opa1L cleavage, leading to cristae opening, decreased OXPHOS, and triggering of mitochondrial fragmentation after reduction in their chaperone function.
Title: Stoichiometric expression of mtHsp40 and mtHsp70 modulates mitochondrial morphology and cristae structure via Opa1L cleavage.

TID1 prevents complex I aggregation and support the existence of a TID1-mediated stress response to ATP synthase inhibition
Title: Essential role of TID1 in maintaining mitochondrial membrane potential homogeneity and mitochondrial DNA integrity.

Tid1 acts as a tumor suppressor by inhibiting EGFR signaling through interaction with EGFR/HSP70/HSP90 and enhancing EGFR ubiquitinylation and degradation.
Title: Tid1-L inhibits EGFR signaling in lung adenocarcinoma by enhancing EGFR Ubiquitinylation and degradation.

The following DNAJA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DNAJA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26971	NM_001135110.3 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu27322	NM_005147.6 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$204.50-$286.30 $409.00
OHu07907	NM_001286516.1 Latest version!	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu27322	NM_005147.5	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$204.50-$286.30 $409.00
OHu26971	NM_001135110.2	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DNAJA3 ( NM_001135110.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128582.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26971
Clone ID Related Accession (Same CDS sequence)	NM_001135110.2 , NM_001135110.3
Accession Version	NM_001135110.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily A member 3, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX445320.2, DA172592.1, AK001333.1 and BC020248.1. On Aug 31, 2011 this sequence version replaced NM_001135110.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2, also known as TID1S) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF411044.1, SRR1660809.58231.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG AAAAAGATCC ACTGGAAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128582.1
CDS	78..1439
Translation	MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRSTGN

Target ORF information:

RefSeq Version	NM_001135110.2
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135110.2

ORF Insert Sequence:

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG AAAAAGATCC ACTGGAAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJA3 ( NM_001135110.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128582.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26971
Clone ID Related Accession (Same CDS sequence)	NM_001135110.2 , NM_001135110.3
Accession Version	NM_001135110.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1362bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily A member 3, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA172592.1, AK001333.1 and BC020248.1. On May 31, 2019 this sequence version replaced NM_001135110.2. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2, also known as TID1S) is shorter and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.263417.1, SRR1660809.65753.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG AAAAAGATCC ACTGGAAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128582.1
CDS	15..1376
Translation	MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRSTGN

Target ORF information:

RefSeq Version	NM_001135110.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135110.3

ORF Insert Sequence:

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG AAAAAGATCC ACTGGAAACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJA3 ( NM_005147.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005138.3 Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27322
Clone ID Related Accession (Same CDS sequence)	NM_005147.6 , NM_005147.5
Accession Version	NM_005147.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily A member 3, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK223105.1, BC011855.2 and AB209923.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005147.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as TID1L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.495647.1, SRR3476690.1054958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000262375.11/ ENSP00000262375.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG TGGCAGCACC ATGGATAGCT CCGCAGGAAG CAAGGCTAGG 
CGTGAGGCTG GGGAGGACGA GGAGGGATTC CTTTCCAAAC TTAAGAAAAT GTTTACCTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005138.3
CDS	15..1457
Translation	MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS

Target ORF information:

RefSeq Version	NM_005147.6
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005147.6

ORF Insert Sequence:

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG TGGCAGCACC ATGGATAGCT CCGCAGGAAG CAAGGCTAGG 
CGTGAGGCTG GGGAGGACGA GGAGGGATTC CTTTCCAAAC TTAAGAAAAT GTTTACCTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJA3 ( NM_005147.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005138.3 Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 1, mRNA.

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CloneID	OHu27322
Clone ID Related Accession (Same CDS sequence)	NM_005147.6 , NM_005147.5
Accession Version	NM_005147.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1443bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily A member 3, mitochondrial isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX445320.2, AK223105.1, BC011855.2 and AB209923.1. This sequence is a reference standard in the RefSeqGene project. On Aug 31, 2011 this sequence version replaced NM_005147.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1, also known as TID1L). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC011855.2, SRR1803616.237819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG TGGCAGCACC ATGGATAGCT CCGCAGGAAG CAAGGCTAGG 
CGTGAGGCTG GGGAGGACGA GGAGGGATTC CTTTCCAAAC TTAAGAAAAT GTTTACCTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005138.3
CDS	78..1520
Translation	MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS

Target ORF information:

RefSeq Version	NM_005147.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005147.5

ORF Insert Sequence:

ATGGCTGCGC GGTGCTCCAC ACGCTGGTTG CTGGTGGTTG TGGGGACCCC GCGGCTGCCG 
GCTATATCGG GTAGAGGGGC CCGGCCGCCC AGGGAGGGCG TGGTGGGGGC ATGGCTGAGC 
CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC CCGAGCGCTG 
CTGACATTGA GACCTGGTGT CAGCCTTACA GGAACAAAAC ATAACCCTTT CATTTGTACT 
GCCTCCTTCC ACACGAGTGC CCCTTTGGCC AAAGAAGATT ATTATCAGAT ATTAGGAGTG 
CCTCGAAATG CCAGCCAGAA AGAGATCAAG AAAGCCTATT ATCAGCTTGC CAAGAAGTAT 
CACCCTGACA CAAATAAGGA TGATCCCAAA GCCAAGGAGA AGTTCTCCCA GCTGGCAGAA 
GCCTATGAGG TTTTGAGTGA TGAGGTGAAG AGGAAGCAGT ACGATGCCTA CGGCTCTGCA 
GGCTTCGATC CTGGGGCCAG CGGCTCCCAG CATAGCTACT GGAAGGGAGG CCCCACTGTG 
GACCCCGAGG AGCTGTTCAG GAAGATCTTT GGCGAGTTCT CATCCTCTTC ATTTGGAGAT 
TTCCAGACCG TGTTTGATCA GCCTCAGGAA TACTTCATGG AGTTGACATT CAATCAAGCT 
GCAAAGGGGG TCAACAAGGA GTTCACCGTG AACATCATGG ACACGTGTGA GCGCTGCAAC 
GGCAAGGGGA ACGAGCCCGG CACCAAGGTG CAGCATTGCC ACTACTGTGG CGGCTCCGGC 
ATGGAAACCA TCAACACAGG CCCTTTTGTG ATGCGTTCCA CGTGTAGGAG ATGTGGTGGC 
CGCGGCTCCA TCATCATATC GCCCTGTGTG GTCTGCAGGG GAGCAGGACA AGCCAAGCAG 
AAAAAGCGAG TGATGATCCC TGTGCCTGCA GGAGTCGAGG ATGGCCAGAC CGTGAGGATG 
CCTGTGGGAA AAAGGGAAAT TTTCATTACG TTCAGGGTGC AGAAAAGCCC TGTGTTCCGG 
AGGGACGGCG CAGACATCCA CTCCGACCTC TTTATTTCTA TAGCTCAGGC TCTTCTTGGG 
GGTACAGCCA GAGCCCAGGG CCTGTACGAG ACGATCAACG TGACGATCCC CCCTGGGACT 
CAGACAGACC AGAAGATTCG GATGGGTGGG AAAGGCATCC CCCGGATTAA CAGCTACGGC 
TACGGAGACC ACTACATCCA CATCAAGATA CGAGTTCCAA AGAGGCTAAC GAGCCGGCAG 
CAGAGCCTGA TCCTGAGCTA CGCCGAGGAC GAGACAGATG TGGAGGGGAC GGTGAACGGC 
GTCACCCTCA CCAGCTCTGG TGGCAGCACC ATGGATAGCT CCGCAGGAAG CAAGGCTAGG 
CGTGAGGCTG GGGAGGACGA GGAGGGATTC CTTTCCAAAC TTAAGAAAAT GTTTACCTCA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DNAJA3 ( NM_001286516.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273445.1 Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07907
Clone ID Related Accession (Same CDS sequence)	NM_001286516.1
Accession Version	NM_001286516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 903bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	dnaJ homolog subfamily A member 3, mitochondrial isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX445320.2, AC012676.5, AK295391.1, AK001333.1 and BC011855.2. On Nov 7, 2013 this sequence version replaced XM_005255677.1. Transcript Variant: This variant (3) lacks a segment in the 5' end and an alternate exon in the 3' end compared to variant 1. The resulting isoform (3) has shorter and distinct N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295391.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAGC CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC 
CCGAGCGCTG CTGACATTGA GACCTGGTGT CAGCCTTACA GGAAGATCTT TGGCGAGTTC 
TCATCCTCTT CATTTGGAGA TTTCCAGACC GTGTTTGATC AGCCTCAGGA ATACTTCATG 
GAGTTGACAT TCAATCAAGC TGCAAAGGGG GTCAACAAGG AGTTCACCGT GAACATCATG 
GACACGTGTG AGCGCTGCAA CGGCAAGGGG AACGAGCCCG GCACCAAGGT GCAGCATTGC 
CACTACTGTG GCGGCTCCGG CATGGAAACC ATCAACACAG GCCCTTTTGT GATGCGTTCC 
ACGTGTAGGA GATGTGGTGG CCGCGGCTCC ATCATCATAT CGCCCTGTGT GGTCTGCAGG 
GGAGCAGGAC AAGCCAAGCA GAAAAAGCGA GTGATGATCC CTGTGCCTGC AGGAGTCGAG 
GATGGCCAGA CCGTGAGGAT GCCTGTGGGA AAAAGGGAAA TTTTCATTAC GTTCAGGGTG 
CAGAAAAGCC CTGTGTTCCG GAGGGACGGC GCAGACATCC ACTCCGACCT CTTTATTTCT 
ATAGCTCAGG CTCTTCTTGG GGGTACAGCC AGAGCCCAGG GCCTGTACGA GACGATCAAC 
GTGACGATCC CCCCTGGGAC TCAGACAGAC CAGAAGATTC GGATGGGTGG GAAAGGCATC 
CCCCGGATTA ACAGCTACGG CTACGGAGAC CACTACATCC ACATCAAGAT ACGAGTTCCA 
AAGAGGCTAA CGAGCCGGCA GCAGAGCCTG ATCCTGAGCT ACGCCGAGGA CGAGACAGAT 
GTGGAGGGGA CGGTGAACGG CGTCACCCTC ACCAGCTCTG GAAAAAGATC CACTGGAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273445.1
CDS	188..1090
Translation	MAEPQAERPRLCVFPDLLRPPSAADIETWCQPYRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRSTGN

Target ORF information:

RefSeq Version	NM_001286516.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DnaJ heat shock protein family (Hsp40) member A3 (DNAJA3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286516.1

ORF Insert Sequence:

ATGGCTGAGC CGCAAGCTGA GCGTCCCCGC CTTTGCGTCT TCCCTGACCT CTTGCGGCCC 
CCGAGCGCTG CTGACATTGA GACCTGGTGT CAGCCTTACA GGAAGATCTT TGGCGAGTTC 
TCATCCTCTT CATTTGGAGA TTTCCAGACC GTGTTTGATC AGCCTCAGGA ATACTTCATG 
GAGTTGACAT TCAATCAAGC TGCAAAGGGG GTCAACAAGG AGTTCACCGT GAACATCATG 
GACACGTGTG AGCGCTGCAA CGGCAAGGGG AACGAGCCCG GCACCAAGGT GCAGCATTGC 
CACTACTGTG GCGGCTCCGG CATGGAAACC ATCAACACAG GCCCTTTTGT GATGCGTTCC 
ACGTGTAGGA GATGTGGTGG CCGCGGCTCC ATCATCATAT CGCCCTGTGT GGTCTGCAGG 
GGAGCAGGAC AAGCCAAGCA GAAAAAGCGA GTGATGATCC CTGTGCCTGC AGGAGTCGAG 
GATGGCCAGA CCGTGAGGAT GCCTGTGGGA AAAAGGGAAA TTTTCATTAC GTTCAGGGTG 
CAGAAAAGCC CTGTGTTCCG GAGGGACGGC GCAGACATCC ACTCCGACCT CTTTATTTCT 
ATAGCTCAGG CTCTTCTTGG GGGTACAGCC AGAGCCCAGG GCCTGTACGA GACGATCAAC 
GTGACGATCC CCCCTGGGAC TCAGACAGAC CAGAAGATTC GGATGGGTGG GAAAGGCATC 
CCCCGGATTA ACAGCTACGG CTACGGAGAC CACTACATCC ACATCAAGAT ACGAGTTCCA 
AAGAGGCTAA CGAGCCGGCA GCAGAGCCTG ATCCTGAGCT ACGCCGAGGA CGAGACAGAT 
GTGGAGGGGA CGGTGAACGG CGTCACCCTC ACCAGCTCTG GAAAAAGATC CACTGGAAAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Role of the Phylogenetically Conserved Cochaperone Protein Droj2/DNAJA3 in NF-?B Signaling.
The Journal of biological chemistry290(39)23816-25(2015 Sep)
Momiuchi Y,Kumada K,Kuraishi T,Takagaki T,Aigaki T,Oshima Y,Kurata S

Tid1, the Mammalian Homologue of Drosophila Tumor Suppressor Tid56, Mediates Macroautophagy by Interacting with Beclin1-containing Autophagy Protein Complex.
The Journal of biological chemistry290(29)18102-10(2015 Jul)
Niu G,Zhang H,Liu D,Chen L,Belani C,Wang HG,Cheng H

Stoichiometric expression of mtHsp40 and mtHsp70 modulates mitochondrial morphology and cristae structure via Opa1L cleavage.
Molecular biology of the cell26(12)2156-67(2015 Jun)
Lee B,Ahn Y,Kang SM,Park Y,Jeon YJ,Rho JM,Kim SW

Tid1 is a new regulator of p53 mitochondrial translocation and apoptosis in cancer.
Oncogene29(8)1155-66(2010 Feb)
Ahn BY,Trinh DL,Zajchowski LD,Lee B,Elwi AN,Kim SW

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DNAJA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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