SART1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SART1
Entrez Gene ID	9092
Full Name	SART1, U4/U6.U5 tri-snRNP-associated protein 1
Synonyms	Ara1,HAF,HOMS1,SART1259,SNRNP110,Snu66
General protein information	Preferred Names SART1, U4/U6.U5 tri-snRNP-associated protein 1 Names U4/U6.U5 tri-snRNP-associated protein 1 IgE autoantigen SART-1 SART1(259) protein SART1(800) protein SNU66 homolog U4/U6.U5 tri-snRNP-associated 110 kDa protein hSART-1 hSnu66 hypoxia associated factor small nuclear ribonucleoprotein 110kDa (U4/U6.U5) squamous cell carcinoma antigen recognised by T cells squamous cell carcinoma antigen recognized by T-cells 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.1
Summary	This gene encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full-length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor-rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri-snRNP to the pre-spliceosome in the spliceosome assembly pathway. [provided by RefSeq, Jul 2008].
Disorder MIM:	605941

mRNA and Protein(s)

mRNA	Protein	Name
XM_011545345.2	XP_011543647.1	U4/U6.U5 tri-snRNP-associated protein 1 isoform X2
NM_005146.4	NP_005137.1	U4/U6.U5 tri-snRNP-associated protein 1
NM_005146.5	NP_005137.1	U4/U6.U5 tri-snRNP-associated protein 1
XM_011545344.1	XP_011543646.1	U4/U6.U5 tri-snRNP-associated protein 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SART1	NP_005137.1
Danio rerio (zebrafish)	sart1	NP_001002673.1
Pan troglodytes (chimpanzee)	SART1	XP_003313187.1
Canis lupus familiaris (dog)	SART1	XP_005631804.1
Xenopus tropicalis (tropical clawed frog)	sart1	XP_002940547.2
Bos taurus (cattle)	SART1	NP_001180015.1
Rattus norvegicus (Norway rat)	Sart1	NP_113784.1
Mus musculus (house mouse)	Sart1	NP_058578.3

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Bertram K, Agafonov DE, Dybkov O, Haselbach D, Leelaram MN, Will CL, Urlaub H, Kastner B, L?hrmann R, Stark H
Cell170(4)701-713.e11(2017 Aug)

Mutant versions of von Hippel-Lindau (VHL) can protect HIF1? from SART1-mediated degradation in clear-cell renal cell carcinoma.
Ord??ez-Navadijo ?, Fuertes-Yebra E, Acosta-Iborra B, Balsa E, Elorza A, Aragon?s J, Landazuri MO
Oncogene35(5)587-94(2016 Feb)

Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.
Li Y, Zhu C, Wang F, Zhu T, Li J, Liu S, Xiao F
Mediators of inflammation20163894816(2016)

The spliceosome factor SART1 exerts its anti-HCV action through mRNA splicing.
Lin W, Zhu C, Hong J, Zhao L, Jilg N, Fusco DN, Schaefer EA, Brisac C, Liu X, Peng LF, Xu Q, Chung RT
Journal of hepatology62(5)1024-32(2015 May)

UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells.
Oka Y, Varmark H, Vitting-Seerup K, Beli P, Waage J, Hakobyan A, Mistrik M, Choudhary C, Rohde M, Bekker-Jensen S, Mailand N
EMBO reports15(9)956-64(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that mutant VHL can protect HIF1alpha from SART1-dependent degradation in normoxic conditions, but this protection is lost in hypoxic settings, favoring hypoxia-dependent ccRCC proliferation.
Title: Mutant versions of von Hippel-Lindau (VHL) can protect HIF1? from SART1-mediated degradation in clear-cell renal cell carcinoma.

SART1 has a role in IFN-mediated anti-Hepatitis B virus response
Title: Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.

SART1 exerts its anti-Hepatitis C virus action through direct transcriptional regulation for some Interferon stimulating genes and alternative splicing for others.
Title: The spliceosome factor SART1 exerts its anti-HCV action through mRNA splicing.

This study identifies SART1 as a previously unidentified regulator of c-FLIP and drug-induced activation of caspase-8.
Title: A systems biology approach identifies SART1 as a novel determinant of both 5-fluorouracil and SN38 drug resistance in colorectal cancer.

Observational study of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Association of mitotic regulation pathway polymorphisms with pancreatic cancer risk and outcome.

The following SART1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SART1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30856	NM_005146.5 Latest version!	Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu62455	XM_011545345.2 Latest version!	Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu62454	XM_011545344.1 Latest version!	Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu30856	NM_005146.4	Homo sapiens SART1, U4/U6.U5 tri-snRNP-associated protein 1 (SART1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SART1 ( NM_005146.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005137.1 Homo sapiens SART1, U4/U6.U5 tri-snRNP-associated protein 1 (SART1), mRNA.

Price & Availability↑

CloneID	OHu30856
Clone ID Related Accession (Same CDS sequence)	NM_005146.4 , NM_005146.5
Accession Version	NM_005146.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	U4/U6.U5 tri-snRNP-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU136920.1, AB006198.1 and AP006287.2. On Dec 14, 2006 this sequence version replaced NM_005146.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.130432.1, SRR1660807.226377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTCGT CCAAGAAGCA TCGCGGAGAG AAGGAGGCGG CCGGGACGAC GGCGGCGGCC 
GGCACCGGGG GTGCCACCGA GCAGCCGCCG CGGCACCGGG AACACAAAAA ACACAAGCAC 
CGGAGTGGCG GCAGTGGCGG TAGCGGTGGC GAACGACGGA AGCGGAGCCG GGAACGTGGG 
GGCGAGCGCG GGAGCGGGCG GCGCGGGGCC GAAGCTGAGG CCCGGAGCAG CACGCACGGG 
CGGGAGCGCA GCCAGGCAGA GCCCTCCGAG CGGCGCGTGA AGCGGGAGAA GCGCGATGAC 
GGCTACGAGG CCGCTGCCAG CTCCAAAACT AGCTCAGGCG ATGCCTCCTC ACTCAGCATC 
GAGGAGACTA ACAAACTCCG GGCAAAGTTG GGGCTGAAAC CCTTGGAGGT TAATGCCATC 
AAGAAGGAGG CGGGCACCAA GGAGGAGCCC GTGACAGCTG ATGTCATCAA CCCTATGGCC 
TTGCGACAGC GAGAGGAGCT GCGGGAGAAG CTGGCGGCTG CCAAGGAGAA GCGCCTGCTG 
AACCAAAAGC TGGGGAAGAT AAAGACCCTA GGAGAGGATG ACCCCTGGCT GGACGACACT 
GCAGCCTGGA TCGAGAGGAG CCGGCAGCTG CAGAAGGAGA AGGACCTGGC AGAGAAGAGG 
GCCAAGTTAC TGGAGGAGAT GGACCAAGAG TTTGGTGTCA GCACTCTGGT GGAGGAGGAG 
TTCGGGCAGA GGCGGCAGGA CCTGTACAGT GCCCGGGACC TGCAGGGCCT CACCGTGGAG 
CATGCCATTG ATTCCTTCCG AGAAGGGGAG ACAATGATTC TTACCCTCAA GGACAAAGGC 
GTGCTGCAGG AGGAGGAGGA CGTGCTGGTG AACGTGAACC TGGTGGATAA GGAGCGGGCA 
GAGAAAAATG TGGAGCTGCG GAAGAAGAAG CCTGACTACC TGCCCTATGC CGAGGACGAG 
AGCGTGGACG ACCTGGCGCA GCAAAAACCT CGCTCTATCC TGTCCAAGTA TGACGAAGAG 
CTTGAAGGGG AGCGGCCACA TTCCTTCCGC TTGGAGCAGG GCGGCACGGC TGATGGCCTG 
CGGGAGCGGG AGCTGGAGGA GATCCGGGCC AAGCTGCGGC TGCAGGCTCA GTCCCTGAGC 
ACAGTGGGGC CCCGGCTGGC CTCCGAATAC CTCACGCCTG AGGAGATGGT GACCTTTAAA 
AAGACCAAGC GGAGGGTGAA GAAAATCCGC AAGAAGGAGA AGGAGGTAGT AGTGCGGGCA 
GATGACTTGC TGCCTCTCGG GGACCAGACT CAGGATGGGG ACTTTGGTTC CAGACTGCGG 
GGACGGGGTC GCCGCCGAGT GTCCGAAGTG GAGGAGGAGA AGGAGCCTGT GCCTCAGCCC 
CTGCCGTCGG ACGACACCCG AGTGGAGAAC ATGGACATCA GTGATGAGGA GGAAGGTGGA 
GCTCCACCGC CGGGGTCCCC GCAGGTGCTG GAGGAGGACG AGGCGGAGCT GGAGCTGCAG 
AAGCAGCTGG AGAAGGGACG CCGGCTGCGA CAGTTACAGC AGCTACAGCA GCTGCGAGAC 
AGTGGCGAGA AGGTGGTGGA GATTGTGAAG AAGCTGGAGT CTCGCCAGCG GGGCTGGGAG 
GAGGATGAGG ATCCCGAGCG GAAGGGGGCC ATCGTGTTCA ACGCCACGTC CGAGTTCTGC 
CGCACCTTGG GGGAGATCCC CACCTACGGG CTGGCTGGCA ATCGCGAGGA GCAGGAGGAG 
CTCATGGACT TTGAACGGGA TGAGGAGCGC TCAGCCAACG GTGGCTCCGA ATCTGACGGG 
GAGGAGAACA TCGGCTGGAG CACGGTGAAC CTGGACGAGG AGAAGCAGCA GCAGGATTTC 
TCTGCTTCCT CCACCACCAT CCTGGACGAG GAACCGATCG TGAATAGGGG GCTGGCAGCT 
GCCCTGCTCC TGTGTCAGAA CAAAGGGCTG CTGGAGACCA CAGTGCAGAA GGTGGCCCGG 
GTGAAGGCCC CCAACAAGTC GCTGCCCTCA GCCGTGTACT GCATCGAGGA TAAGATGGCC 
ATCGATGACA AGTACAGCCG GAGGGAGGAA TACCGAGGCT TCACACAGGA CTTCAAGGAG 
AAGGACGGCT ACAAACCCGA CGTTAAGATC GAATACGTGG ATGAGACGGG CCGGAAACTC 
ACACCCAAGG AGGCTTTCCG GCAGCTGTCG CACCGCTTCC ATGGCAAGGG CTCAGGCAAG 
ATGAAGACAG AGCGGCGGAT GAAGAAGCTG GACGAGGAGG CGCTCCTGAA GAAGATGAGC 
TCCAGCGACA CGCCCCTGGG CACCGTGGCC CTGCTCCAGG AGAAGCAGAA GGCTCAGAAG 
ACCCCCTACA TCGTGCTCAG CGGCAGCGGC AAGAGCATGA ACGCGAACAC CATCACCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005137.1
CDS	93..2495
Translation	MGSSKKHRGEKEAAGTTAAAGTGGATEQPPRHREHKKHKHRSGGSGGSGGERRKRSRERGGERGSGRRGAEAEARSSTHGRERSQAEPSERRVKREKRDDGYEAAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAIKKEAGTKEEPVTADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	NM_005146.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SART1, U4/U6.U5 tri-snRNP-associated protein 1 (SART1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005146.4

ORF Insert Sequence:

ATGGGGTCGT CCAAGAAGCA TCGCGGAGAG AAGGAGGCGG CCGGGACGAC GGCGGCGGCC 
GGCACCGGGG GTGCCACCGA GCAGCCGCCG CGGCACCGGG AACACAAAAA ACACAAGCAC 
CGGAGTGGCG GCAGTGGCGG TAGCGGTGGC GAACGACGGA AGCGGAGCCG GGAACGTGGG 
GGCGAGCGCG GGAGCGGGCG GCGCGGGGCC GAAGCTGAGG CCCGGAGCAG CACGCACGGG 
CGGGAGCGCA GCCAGGCAGA GCCCTCCGAG CGGCGCGTGA AGCGGGAGAA GCGCGATGAC 
GGCTACGAGG CCGCTGCCAG CTCCAAAACT AGCTCAGGCG ATGCCTCCTC ACTCAGCATC 
GAGGAGACTA ACAAACTCCG GGCAAAGTTG GGGCTGAAAC CCTTGGAGGT TAATGCCATC 
AAGAAGGAGG CGGGCACCAA GGAGGAGCCC GTGACAGCTG ATGTCATCAA CCCTATGGCC 
TTGCGACAGC GAGAGGAGCT GCGGGAGAAG CTGGCGGCTG CCAAGGAGAA GCGCCTGCTG 
AACCAAAAGC TGGGGAAGAT AAAGACCCTA GGAGAGGATG ACCCCTGGCT GGACGACACT 
GCAGCCTGGA TCGAGAGGAG CCGGCAGCTG CAGAAGGAGA AGGACCTGGC AGAGAAGAGG 
GCCAAGTTAC TGGAGGAGAT GGACCAAGAG TTTGGTGTCA GCACTCTGGT GGAGGAGGAG 
TTCGGGCAGA GGCGGCAGGA CCTGTACAGT GCCCGGGACC TGCAGGGCCT CACCGTGGAG 
CATGCCATTG ATTCCTTCCG AGAAGGGGAG ACAATGATTC TTACCCTCAA GGACAAAGGC 
GTGCTGCAGG AGGAGGAGGA CGTGCTGGTG AACGTGAACC TGGTGGATAA GGAGCGGGCA 
GAGAAAAATG TGGAGCTGCG GAAGAAGAAG CCTGACTACC TGCCCTATGC CGAGGACGAG 
AGCGTGGACG ACCTGGCGCA GCAAAAACCT CGCTCTATCC TGTCCAAGTA TGACGAAGAG 
CTTGAAGGGG AGCGGCCACA TTCCTTCCGC TTGGAGCAGG GCGGCACGGC TGATGGCCTG 
CGGGAGCGGG AGCTGGAGGA GATCCGGGCC AAGCTGCGGC TGCAGGCTCA GTCCCTGAGC 
ACAGTGGGGC CCCGGCTGGC CTCCGAATAC CTCACGCCTG AGGAGATGGT GACCTTTAAA 
AAGACCAAGC GGAGGGTGAA GAAAATCCGC AAGAAGGAGA AGGAGGTAGT AGTGCGGGCA 
GATGACTTGC TGCCTCTCGG GGACCAGACT CAGGATGGGG ACTTTGGTTC CAGACTGCGG 
GGACGGGGTC GCCGCCGAGT GTCCGAAGTG GAGGAGGAGA AGGAGCCTGT GCCTCAGCCC 
CTGCCGTCGG ACGACACCCG AGTGGAGAAC ATGGACATCA GTGATGAGGA GGAAGGTGGA 
GCTCCACCGC CGGGGTCCCC GCAGGTGCTG GAGGAGGACG AGGCGGAGCT GGAGCTGCAG 
AAGCAGCTGG AGAAGGGACG CCGGCTGCGA CAGTTACAGC AGCTACAGCA GCTGCGAGAC 
AGTGGCGAGA AGGTGGTGGA GATTGTGAAG AAGCTGGAGT CTCGCCAGCG GGGCTGGGAG 
GAGGATGAGG ATCCCGAGCG GAAGGGGGCC ATCGTGTTCA ACGCCACGTC CGAGTTCTGC 
CGCACCTTGG GGGAGATCCC CACCTACGGG CTGGCTGGCA ATCGCGAGGA GCAGGAGGAG 
CTCATGGACT TTGAACGGGA TGAGGAGCGC TCAGCCAACG GTGGCTCCGA ATCTGACGGG 
GAGGAGAACA TCGGCTGGAG CACGGTGAAC CTGGACGAGG AGAAGCAGCA GCAGGATTTC 
TCTGCTTCCT CCACCACCAT CCTGGACGAG GAACCGATCG TGAATAGGGG GCTGGCAGCT 
GCCCTGCTCC TGTGTCAGAA CAAAGGGCTG CTGGAGACCA CAGTGCAGAA GGTGGCCCGG 
GTGAAGGCCC CCAACAAGTC GCTGCCCTCA GCCGTGTACT GCATCGAGGA TAAGATGGCC 
ATCGATGACA AGTACAGCCG GAGGGAGGAA TACCGAGGCT TCACACAGGA CTTCAAGGAG 
AAGGACGGCT ACAAACCCGA CGTTAAGATC GAATACGTGG ATGAGACGGG CCGGAAACTC 
ACACCCAAGG AGGCTTTCCG GCAGCTGTCG CACCGCTTCC ATGGCAAGGG CTCAGGCAAG 
ATGAAGACAG AGCGGCGGAT GAAGAAGCTG GACGAGGAGG CGCTCCTGAA GAAGATGAGC 
TCCAGCGACA CGCCCCTGGG CACCGTGGCC CTGCTCCAGG AGAAGCAGAA GGCTCAGAAG 
ACCCCCTACA TCGTGCTCAG CGGCAGCGGC AAGAGCATGA ACGCGAACAC CATCACCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SART1 ( NM_005146.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005137.1 Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), mRNA.

Price & Availability↑

CloneID	OHu30856
Clone ID Related Accession (Same CDS sequence)	NM_005146.4 , NM_005146.5
Accession Version	NM_005146.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2403bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	U4/U6.U5 tri-snRNP-associated protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU136920.1, AB006198.1 and AP006287.2. On Nov 22, 2018 this sequence version replaced NM_005146.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.130432.1, SRR1660807.226377.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312397.10/ ENSP00000310448.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGTCGT CCAAGAAGCA TCGCGGAGAG AAGGAGGCGG CCGGGACGAC GGCGGCGGCC 
GGCACCGGGG GTGCCACCGA GCAGCCGCCG CGGCACCGGG AACACAAAAA ACACAAGCAC 
CGGAGTGGCG GCAGTGGCGG TAGCGGTGGC GAACGACGGA AGCGGAGCCG GGAACGTGGG 
GGCGAGCGCG GGAGCGGGCG GCGCGGGGCC GAAGCTGAGG CCCGGAGCAG CACGCACGGG 
CGGGAGCGCA GCCAGGCAGA GCCCTCCGAG CGGCGCGTGA AGCGGGAGAA GCGCGATGAC 
GGCTACGAGG CCGCTGCCAG CTCCAAAACT AGCTCAGGCG ATGCCTCCTC ACTCAGCATC 
GAGGAGACTA ACAAACTCCG GGCAAAGTTG GGGCTGAAAC CCTTGGAGGT TAATGCCATC 
AAGAAGGAGG CGGGCACCAA GGAGGAGCCC GTGACAGCTG ATGTCATCAA CCCTATGGCC 
TTGCGACAGC GAGAGGAGCT GCGGGAGAAG CTGGCGGCTG CCAAGGAGAA GCGCCTGCTG 
AACCAAAAGC TGGGGAAGAT AAAGACCCTA GGAGAGGATG ACCCCTGGCT GGACGACACT 
GCAGCCTGGA TCGAGAGGAG CCGGCAGCTG CAGAAGGAGA AGGACCTGGC AGAGAAGAGG 
GCCAAGTTAC TGGAGGAGAT GGACCAAGAG TTTGGTGTCA GCACTCTGGT GGAGGAGGAG 
TTCGGGCAGA GGCGGCAGGA CCTGTACAGT GCCCGGGACC TGCAGGGCCT CACCGTGGAG 
CATGCCATTG ATTCCTTCCG AGAAGGGGAG ACAATGATTC TTACCCTCAA GGACAAAGGC 
GTGCTGCAGG AGGAGGAGGA CGTGCTGGTG AACGTGAACC TGGTGGATAA GGAGCGGGCA 
GAGAAAAATG TGGAGCTGCG GAAGAAGAAG CCTGACTACC TGCCCTATGC CGAGGACGAG 
AGCGTGGACG ACCTGGCGCA GCAAAAACCT CGCTCTATCC TGTCCAAGTA TGACGAAGAG 
CTTGAAGGGG AGCGGCCACA TTCCTTCCGC TTGGAGCAGG GCGGCACGGC TGATGGCCTG 
CGGGAGCGGG AGCTGGAGGA GATCCGGGCC AAGCTGCGGC TGCAGGCTCA GTCCCTGAGC 
ACAGTGGGGC CCCGGCTGGC CTCCGAATAC CTCACGCCTG AGGAGATGGT GACCTTTAAA 
AAGACCAAGC GGAGGGTGAA GAAAATCCGC AAGAAGGAGA AGGAGGTAGT AGTGCGGGCA 
GATGACTTGC TGCCTCTCGG GGACCAGACT CAGGATGGGG ACTTTGGTTC CAGACTGCGG 
GGACGGGGTC GCCGCCGAGT GTCCGAAGTG GAGGAGGAGA AGGAGCCTGT GCCTCAGCCC 
CTGCCGTCGG ACGACACCCG AGTGGAGAAC ATGGACATCA GTGATGAGGA GGAAGGTGGA 
GCTCCACCGC CGGGGTCCCC GCAGGTGCTG GAGGAGGACG AGGCGGAGCT GGAGCTGCAG 
AAGCAGCTGG AGAAGGGACG CCGGCTGCGA CAGTTACAGC AGCTACAGCA GCTGCGAGAC 
AGTGGCGAGA AGGTGGTGGA GATTGTGAAG AAGCTGGAGT CTCGCCAGCG GGGCTGGGAG 
GAGGATGAGG ATCCCGAGCG GAAGGGGGCC ATCGTGTTCA ACGCCACGTC CGAGTTCTGC 
CGCACCTTGG GGGAGATCCC CACCTACGGG CTGGCTGGCA ATCGCGAGGA GCAGGAGGAG 
CTCATGGACT TTGAACGGGA TGAGGAGCGC TCAGCCAACG GTGGCTCCGA ATCTGACGGG 
GAGGAGAACA TCGGCTGGAG CACGGTGAAC CTGGACGAGG AGAAGCAGCA GCAGGATTTC 
TCTGCTTCCT CCACCACCAT CCTGGACGAG GAACCGATCG TGAATAGGGG GCTGGCAGCT 
GCCCTGCTCC TGTGTCAGAA CAAAGGGCTG CTGGAGACCA CAGTGCAGAA GGTGGCCCGG 
GTGAAGGCCC CCAACAAGTC GCTGCCCTCA GCCGTGTACT GCATCGAGGA TAAGATGGCC 
ATCGATGACA AGTACAGCCG GAGGGAGGAA TACCGAGGCT TCACACAGGA CTTCAAGGAG 
AAGGACGGCT ACAAACCCGA CGTTAAGATC GAATACGTGG ATGAGACGGG CCGGAAACTC 
ACACCCAAGG AGGCTTTCCG GCAGCTGTCG CACCGCTTCC ATGGCAAGGG CTCAGGCAAG 
ATGAAGACAG AGCGGCGGAT GAAGAAGCTG GACGAGGAGG CGCTCCTGAA GAAGATGAGC 
TCCAGCGACA CGCCCCTGGG CACCGTGGCC CTGCTCCAGG AGAAGCAGAA GGCTCAGAAG 
ACCCCCTACA TCGTGCTCAG CGGCAGCGGC AAGAGCATGA ACGCGAACAC CATCACCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005137.1
CDS	48..2450
Translation	MGSSKKHRGEKEAAGTTAAAGTGGATEQPPRHREHKKHKHRSGGSGGSGGERRKRSRERGGERGSGRRGAEAEARSSTHGRERSQAEPSERRVKREKRDDGYEAAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAIKKEAGTKEEPVTADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	NM_005146.5
Organism	Homo sapiens(human)
Definition	Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005146.5

ORF Insert Sequence:

ATGGGGTCGT CCAAGAAGCA TCGCGGAGAG AAGGAGGCGG CCGGGACGAC GGCGGCGGCC 
GGCACCGGGG GTGCCACCGA GCAGCCGCCG CGGCACCGGG AACACAAAAA ACACAAGCAC 
CGGAGTGGCG GCAGTGGCGG TAGCGGTGGC GAACGACGGA AGCGGAGCCG GGAACGTGGG 
GGCGAGCGCG GGAGCGGGCG GCGCGGGGCC GAAGCTGAGG CCCGGAGCAG CACGCACGGG 
CGGGAGCGCA GCCAGGCAGA GCCCTCCGAG CGGCGCGTGA AGCGGGAGAA GCGCGATGAC 
GGCTACGAGG CCGCTGCCAG CTCCAAAACT AGCTCAGGCG ATGCCTCCTC ACTCAGCATC 
GAGGAGACTA ACAAACTCCG GGCAAAGTTG GGGCTGAAAC CCTTGGAGGT TAATGCCATC 
AAGAAGGAGG CGGGCACCAA GGAGGAGCCC GTGACAGCTG ATGTCATCAA CCCTATGGCC 
TTGCGACAGC GAGAGGAGCT GCGGGAGAAG CTGGCGGCTG CCAAGGAGAA GCGCCTGCTG 
AACCAAAAGC TGGGGAAGAT AAAGACCCTA GGAGAGGATG ACCCCTGGCT GGACGACACT 
GCAGCCTGGA TCGAGAGGAG CCGGCAGCTG CAGAAGGAGA AGGACCTGGC AGAGAAGAGG 
GCCAAGTTAC TGGAGGAGAT GGACCAAGAG TTTGGTGTCA GCACTCTGGT GGAGGAGGAG 
TTCGGGCAGA GGCGGCAGGA CCTGTACAGT GCCCGGGACC TGCAGGGCCT CACCGTGGAG 
CATGCCATTG ATTCCTTCCG AGAAGGGGAG ACAATGATTC TTACCCTCAA GGACAAAGGC 
GTGCTGCAGG AGGAGGAGGA CGTGCTGGTG AACGTGAACC TGGTGGATAA GGAGCGGGCA 
GAGAAAAATG TGGAGCTGCG GAAGAAGAAG CCTGACTACC TGCCCTATGC CGAGGACGAG 
AGCGTGGACG ACCTGGCGCA GCAAAAACCT CGCTCTATCC TGTCCAAGTA TGACGAAGAG 
CTTGAAGGGG AGCGGCCACA TTCCTTCCGC TTGGAGCAGG GCGGCACGGC TGATGGCCTG 
CGGGAGCGGG AGCTGGAGGA GATCCGGGCC AAGCTGCGGC TGCAGGCTCA GTCCCTGAGC 
ACAGTGGGGC CCCGGCTGGC CTCCGAATAC CTCACGCCTG AGGAGATGGT GACCTTTAAA 
AAGACCAAGC GGAGGGTGAA GAAAATCCGC AAGAAGGAGA AGGAGGTAGT AGTGCGGGCA 
GATGACTTGC TGCCTCTCGG GGACCAGACT CAGGATGGGG ACTTTGGTTC CAGACTGCGG 
GGACGGGGTC GCCGCCGAGT GTCCGAAGTG GAGGAGGAGA AGGAGCCTGT GCCTCAGCCC 
CTGCCGTCGG ACGACACCCG AGTGGAGAAC ATGGACATCA GTGATGAGGA GGAAGGTGGA 
GCTCCACCGC CGGGGTCCCC GCAGGTGCTG GAGGAGGACG AGGCGGAGCT GGAGCTGCAG 
AAGCAGCTGG AGAAGGGACG CCGGCTGCGA CAGTTACAGC AGCTACAGCA GCTGCGAGAC 
AGTGGCGAGA AGGTGGTGGA GATTGTGAAG AAGCTGGAGT CTCGCCAGCG GGGCTGGGAG 
GAGGATGAGG ATCCCGAGCG GAAGGGGGCC ATCGTGTTCA ACGCCACGTC CGAGTTCTGC 
CGCACCTTGG GGGAGATCCC CACCTACGGG CTGGCTGGCA ATCGCGAGGA GCAGGAGGAG 
CTCATGGACT TTGAACGGGA TGAGGAGCGC TCAGCCAACG GTGGCTCCGA ATCTGACGGG 
GAGGAGAACA TCGGCTGGAG CACGGTGAAC CTGGACGAGG AGAAGCAGCA GCAGGATTTC 
TCTGCTTCCT CCACCACCAT CCTGGACGAG GAACCGATCG TGAATAGGGG GCTGGCAGCT 
GCCCTGCTCC TGTGTCAGAA CAAAGGGCTG CTGGAGACCA CAGTGCAGAA GGTGGCCCGG 
GTGAAGGCCC CCAACAAGTC GCTGCCCTCA GCCGTGTACT GCATCGAGGA TAAGATGGCC 
ATCGATGACA AGTACAGCCG GAGGGAGGAA TACCGAGGCT TCACACAGGA CTTCAAGGAG 
AAGGACGGCT ACAAACCCGA CGTTAAGATC GAATACGTGG ATGAGACGGG CCGGAAACTC 
ACACCCAAGG AGGCTTTCCG GCAGCTGTCG CACCGCTTCC ATGGCAAGGG CTCAGGCAAG 
ATGAAGACAG AGCGGCGGAT GAAGAAGCTG GACGAGGAGG CGCTCCTGAA GAAGATGAGC 
TCCAGCGACA CGCCCCTGGG CACCGTGGCC CTGCTCCAGG AGAAGCAGAA GGCTCAGAAG 
ACCCCCTACA TCGTGCTCAG CGGCAGCGGC AAGAGCATGA ACGCGAACAC CATCACCAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SART1 ( XM_011545345.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543647.1 Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), transcript variant X2, mRNA.

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CloneID	OHu62455
Clone ID Related Accession (Same CDS sequence)	XM_011545345.2
Accession Version	XM_011545345.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1929bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	U4/U6.U5 tri-snRNP-associated protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTGC GACAGCGAGA GGAGCTGCGG GAGAAGCTGG CGGCTGCCAA GGAGAAGCGC 
CTGCTGAACC AAAAGCTGGG GAAGATAAAG ACCCTAGGAG AGGATGACCC CTGGCTGGAC 
GACACTGCAG CCTGGATCGA GAGGAGCCGG CAGCTGCAGA AGGAGAAGGA CCTGGCAGAG 
AAGAGGGCCA AGTTACTGGA GGAGATGGAC CAAGAGTTTG GTGTCAGCAC TCTGGTGGAG 
GAGGAGTTCG GGCAGAGGCG GCAGGACCTG TACAGTGCCC GGGACCTGCA GGGCCTCACC 
GTGGAGCATG CCATTGATTC CTTCCGAGAA GGGGAGACAA TGATTCTTAC CCTCAAGGAC 
AAAGGCGTGC TGCAGGAGGA GGAGGACGTG CTGGTGAACG TGAACCTGGT GGATAAGGAG 
CGGGCAGAGA AAAATGTGGA GCTGCGGAAG AAGAAGCCTG ACTACCTGCC CTATGCCGAG 
GACGAGAGCG TGGACGACCT GGCGCAGCAA AAACCTCGCT CTATCCTGTC CAAGTATGAC 
GAAGAGCTTG AAGGGGAGCG GCCACATTCC TTCCGCTTGG AGCAGGGCGG CACGGCTGAT 
GGCCTGCGGG AGCGGGAGCT GGAGGAGATC CGGGCCAAGC TGCGGCTGCA GGCTCAGTCC 
CTGAGCACAG TGGGGCCCCG GCTGGCCTCC GAATACCTCA CGCCTGAGGA GATGGTGACC 
TTTAAAAAGA CCAAGCGGAG GGTGAAGAAA ATCCGCAAGA AGGAGAAGGA GGTAGTAGTG 
CGGGCAGATG ACTTGCTGCC TCTCGGGGAC CAGACTCAGG ATGGGGACTT TGGTTCCAGA 
CTGCGGGGAC GGGGTCGCCG CCGAGTGTCC GAAGTGGAGG AGGAGAAGGA GCCTGTGCCT 
CAGCCCCTGC CGTCGGACGA CACCCGAGTG GAGAACATGG ACATCAGTGA TGAGGAGGAA 
GGTGGAGCTC CACCGCCGGG GTCCCCGCAG GTGCTGGAGG AGGACGAGGC GGAGCTGGAG 
CTGCAGAAGC AGCTGGAGAA GGGACGCCGG CTGCGACAGT TACAGCAGCT ACAGCAGCTG 
CGAGACAGTG GCGAGAAGGT GGTGGAGATT GTGAAGAAGC TGGAGTCTCG CCAGCGGGGC 
TGGGAGGAGG ATGAGGATCC CGAGCGGAAG GGGGCCATCG TGTTCAACGC CACGTCCGAG 
TTCTGCCGCA CCTTGGGGGA GATCCCCACC TACGGGCTGG CTGGCAATCG CGAGGAGCAG 
GAGGAGCTCA TGGACTTTGA ACGGGATGAG GAGCGCTCAG CCAACGGTGG CTCCGAATCT 
GACGGGGAGG AGAACATCGG CTGGAGCACG GTGAACCTGG ACGAGGAGAA GCAGCAGCAG 
GATTTCTCTG CTTCCTCCAC CACCATCCTG GACGAGGAAC CGATCGTGAA TAGGGGGCTG 
GCAGCTGCCC TGCTCCTGTG TCAGAACAAA GGGCTGCTGG AGACCACAGT GCAGAAGGTG 
GCCCGGGTGA AGGCCCCCAA CAAGTCGCTG CCCTCAGCCG TGTACTGCAT CGAGGATAAG 
ATGGCCATCG ATGACAAGTA CAGCCGGAGG GAGGAATACC GAGGCTTCAC ACAGGACTTC 
AAGGAGAAGG ACGGCTACAA ACCCGACGTT AAGATCGAAT ACGTGGATGA GACGGGCCGG 
AAACTCACAC CCAAGGAGGC TTTCCGGCAG CTGTCGCACC GCTTCCATGG CAAGGGCTCA 
GGCAAGATGA AGACAGAGCG GCGGATGAAG AAGCTGGACG AGGAGGCGCT CCTGAAGAAG 
ATGAGCTCCA GCGACACGCC CCTGGGCACC GTGGCCCTGC TCCAGGAGAA GCAGAAGGCT 
CAGAAGACCC CCTACATCGT GCTCAGCGGC AGCGGCAAGA GCATGAACGC GAACACCATC 
ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543647.1
CDS	359..2287
Translation	MALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	XM_011545345.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545345.2

ORF Insert Sequence:

ATGGCCTTGC GACAGCGAGA GGAGCTGCGG GAGAAGCTGG CGGCTGCCAA GGAGAAGCGC 
CTGCTGAACC AAAAGCTGGG GAAGATAAAG ACCCTAGGAG AGGATGACCC CTGGCTGGAC 
GACACTGCAG CCTGGATCGA GAGGAGCCGG CAGCTGCAGA AGGAGAAGGA CCTGGCAGAG 
AAGAGGGCCA AGTTACTGGA GGAGATGGAC CAAGAGTTTG GTGTCAGCAC TCTGGTGGAG 
GAGGAGTTCG GGCAGAGGCG GCAGGACCTG TACAGTGCCC GGGACCTGCA GGGCCTCACC 
GTGGAGCATG CCATTGATTC CTTCCGAGAA GGGGAGACAA TGATTCTTAC CCTCAAGGAC 
AAAGGCGTGC TGCAGGAGGA GGAGGACGTG CTGGTGAACG TGAACCTGGT GGATAAGGAG 
CGGGCAGAGA AAAATGTGGA GCTGCGGAAG AAGAAGCCTG ACTACCTGCC CTATGCCGAG 
GACGAGAGCG TGGACGACCT GGCGCAGCAA AAACCTCGCT CTATCCTGTC CAAGTATGAC 
GAAGAGCTTG AAGGGGAGCG GCCACATTCC TTCCGCTTGG AGCAGGGCGG CACGGCTGAT 
GGCCTGCGGG AGCGGGAGCT GGAGGAGATC CGGGCCAAGC TGCGGCTGCA GGCTCAGTCC 
CTGAGCACAG TGGGGCCCCG GCTGGCCTCC GAATACCTCA CGCCTGAGGA GATGGTGACC 
TTTAAAAAGA CCAAGCGGAG GGTGAAGAAA ATCCGCAAGA AGGAGAAGGA GGTAGTAGTG 
CGGGCAGATG ACTTGCTGCC TCTCGGGGAC CAGACTCAGG ATGGGGACTT TGGTTCCAGA 
CTGCGGGGAC GGGGTCGCCG CCGAGTGTCC GAAGTGGAGG AGGAGAAGGA GCCTGTGCCT 
CAGCCCCTGC CGTCGGACGA CACCCGAGTG GAGAACATGG ACATCAGTGA TGAGGAGGAA 
GGTGGAGCTC CACCGCCGGG GTCCCCGCAG GTGCTGGAGG AGGACGAGGC GGAGCTGGAG 
CTGCAGAAGC AGCTGGAGAA GGGACGCCGG CTGCGACAGT TACAGCAGCT ACAGCAGCTG 
CGAGACAGTG GCGAGAAGGT GGTGGAGATT GTGAAGAAGC TGGAGTCTCG CCAGCGGGGC 
TGGGAGGAGG ATGAGGATCC CGAGCGGAAG GGGGCCATCG TGTTCAACGC CACGTCCGAG 
TTCTGCCGCA CCTTGGGGGA GATCCCCACC TACGGGCTGG CTGGCAATCG CGAGGAGCAG 
GAGGAGCTCA TGGACTTTGA ACGGGATGAG GAGCGCTCAG CCAACGGTGG CTCCGAATCT 
GACGGGGAGG AGAACATCGG CTGGAGCACG GTGAACCTGG ACGAGGAGAA GCAGCAGCAG 
GATTTCTCTG CTTCCTCCAC CACCATCCTG GACGAGGAAC CGATCGTGAA TAGGGGGCTG 
GCAGCTGCCC TGCTCCTGTG TCAGAACAAA GGGCTGCTGG AGACCACAGT GCAGAAGGTG 
GCCCGGGTGA AGGCCCCCAA CAAGTCGCTG CCCTCAGCCG TGTACTGCAT CGAGGATAAG 
ATGGCCATCG ATGACAAGTA CAGCCGGAGG GAGGAATACC GAGGCTTCAC ACAGGACTTC 
AAGGAGAAGG ACGGCTACAA ACCCGACGTT AAGATCGAAT ACGTGGATGA GACGGGCCGG 
AAACTCACAC CCAAGGAGGC TTTCCGGCAG CTGTCGCACC GCTTCCATGG CAAGGGCTCA 
GGCAAGATGA AGACAGAGCG GCGGATGAAG AAGCTGGACG AGGAGGCGCT CCTGAAGAAG 
ATGAGCTCCA GCGACACGCC CCTGGGCACC GTGGCCCTGC TCCAGGAGAA GCAGAAGGCT 
CAGAAGACCC CCTACATCGT GCTCAGCGGC AGCGGCAAGA GCATGAACGC GAACACCATC 
ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SART1 ( XM_011545344.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543646.1 Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu62454
Clone ID Related Accession (Same CDS sequence)	XM_011545344.1
Accession Version	XM_011545344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	U4/U6.U5 tri-snRNP-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC TTGTTCCAGC TGCCAGCTCC AAAACTAGCT CAGGCGATGC CTCCTCACTC 
AGCATCGAGG AGACTAACAA ACTCCGGGCA AAGTTGGGGC TGAAACCCTT GGAGGTTAAT 
GCCATCAAGA AGGAGGCGGG CACCAAGGAG GAGCCCGTGA CAGCTGATGT CATCAACCCT 
ATGGCCTTGC GACAGCGAGA GGAGCTGCGG GAGAAGCTGG CGGCTGCCAA GGAGAAGCGC 
CTGCTGAACC AAAAGCTGGG GAAGATAAAG ACCCTAGGAG AGGATGACCC CTGGCTGGAC 
GACACTGCAG CCTGGATCGA GAGGAGCCGG CAGCTGCAGA AGGAGAAGGA CCTGGCAGAG 
AAGAGGGCCA AGTTACTGGA GGAGATGGAC CAAGAGTTTG GTGTCAGCAC TCTGGTGGAG 
GAGGAGTTCG GGCAGAGGCG GCAGGACCTG TACAGTGCCC GGGACCTGCA GGGCCTCACC 
GTGGAGCATG CCATTGATTC CTTCCGAGAA GGGGAGACAA TGATTCTTAC CCTCAAGGAC 
AAAGGCGTGC TGCAGGAGGA GGAGGACGTG CTGGTGAACG TGAACCTGGT GGATAAGGAG 
CGGGCAGAGA AAAATGTGGA GCTGCGGAAG AAGAAGCCTG ACTACCTGCC CTATGCCGAG 
GACGAGAGCG TGGACGACCT GGCGCAGCAA AAACCTCGCT CTATCCTGTC CAAGTATGAC 
GAAGAGCTTG AAGGGGAGCG GCCACATTCC TTCCGCTTGG AGCAGGGCGG CACGGCTGAT 
GGCCTGCGGG AGCGGGAGCT GGAGGAGATC CGGGCCAAGC TGCGGCTGCA GGCTCAGTCC 
CTGAGCACAG TGGGGCCCCG GCTGGCCTCC GAATACCTCA CGCCTGAGGA GATGGTGACC 
TTTAAAAAGA CCAAGCGGAG GGTGAAGAAA ATCCGCAAGA AGGAGAAGGA GGTAGTAGTG 
CGGGCAGATG ACTTGCTGCC TCTCGGGGAC CAGACTCAGG ATGGGGACTT TGGTTCCAGA 
CTGCGGGGAC GGGGTCGCCG CCGAGTGTCC GAAGTGGAGG AGGAGAAGGA GCCTGTGCCT 
CAGCCCCTGC CGTCGGACGA CACCCGAGTG GAGAACATGG ACATCAGTGA TGAGGAGGAA 
GGTGGAGCTC CACCGCCGGG GTCCCCGCAG GTGCTGGAGG AGGACGAGGC GGAGCTGGAG 
CTGCAGAAGC AGCTGGAGAA GGGACGCCGG CTGCGACAGT TACAGCAGCT ACAGCAGCTG 
CGAGACAGTG GCGAGAAGGT GGTGGAGATT GTGAAGAAGC TGGAGTCTCG CCAGCGGGGC 
TGGGAGGAGG ATGAGGATCC CGAGCGGAAG GGGGCCATCG TGTTCAACGC CACGTCCGAG 
TTCTGCCGCA CCTTGGGGGA GATCCCCACC TACGGGCTGG CTGGCAATCG CGAGGAGCAG 
GAGGAGCTCA TGGACTTTGA ACGGGATGAG GAGCGCTCAG CCAACGGTGG CTCCGAATCT 
GACGGGGAGG AGAACATCGG CTGGAGCACG GTGAACCTGG ACGAGGAGAA GCAGCAGCAG 
GATTTCTCTG CTTCCTCCAC CACCATCCTG GACGAGGAAC CGATCGTGAA TAGGGGGCTG 
GCAGCTGCCC TGCTCCTGTG TCAGAACAAA GGGCTGCTGG AGACCACAGT GCAGAAGGTG 
GCCCGGGTGA AGGCCCCCAA CAAGTCGCTG CCCTCAGCCG TGTACTGCAT CGAGGATAAG 
ATGGCCATCG ATGACAAGTA CAGCCGGAGG GAGGAATACC GAGGCTTCAC ACAGGACTTC 
AAGGAGAAGG ACGGCTACAA ACCCGACGTT AAGATCGAAT ACGTGGATGA GACGGGCCGG 
AAACTCACAC CCAAGGAGGC TTTCCGGCAG CTGTCGCACC GCTTCCATGG CAAGGGCTCA 
GGCAAGATGA AGACAGAGCG GCGGATGAAG AAGCTGGACG AGGAGGCGCT CCTGAAGAAG 
ATGAGCTCCA GCGACACGCC CCTGGGCACC GTGGCCCTGC TCCAGGAGAA GCAGAAGGCT 
CAGAAGACCC CCTACATCGT GCTCAGCGGC AGCGGCAAGA GCATGAACGC GAACACCATC 
ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543646.1
CDS	416..2524
Translation	MLLLVPAASSKTSSGDASSLSIEETNKLRAKLGLKPLEVNAIKKEAGTKEEPVTADVINPMALRQREELREKLAAAKEKRLLNQKLGKIKTLGEDDPWLDDTAAWIERSRQLQKEKDLAEKRAKLLEEMDQEFGVSTLVEEEFGQRRQDLYSARDLQGLTVEHAIDSFREGETMILTLKDKGVLQEEEDVLVNVNLVDKERAEKNVELRKKKPDYLPYAEDESVDDLAQQKPRSILSKYDEELEGERPHSFRLEQGGTADGLRERELEEIRAKLRLQAQSLSTVGPRLASEYLTPEEMVTFKKTKRRVKKIRKKEKEVVVRADDLLPLGDQTQDGDFGSRLRGRGRRRVSEVEEEKEPVPQPLPSDDTRVENMDISDEEEGGAPPPGSPQVLEEDEAELELQKQLEKGRRLRQLQQLQQLRDSGEKVVEIVKKLESRQRGWEEDEDPERKGAIVFNATSEFCRTLGEIPTYGLAGNREEQEELMDFERDEERSANGGSESDGEENIGWSTVNLDEEKQQQDFSASSTTILDEEPIVNRGLAAALLLCQNKGLLETTVQKVARVKAPNKSLPSAVYCIEDKMAIDDKYSRREEYRGFTQDFKEKDGYKPDVKIEYVDETGRKLTPKEAFRQLSHRFHGKGSGKMKTERRMKKLDEEALLKKMSSSDTPLGTVALLQEKQKAQKTPYIVLSGSGKSMNANTITK

Target ORF information:

RefSeq Version	XM_011545344.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spliceosome associated factor 1, recruiter of U4/U6.U5 tri-snRNP (SART1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545344.1

ORF Insert Sequence:

ATGCTTCTTC TTGTTCCAGC TGCCAGCTCC AAAACTAGCT CAGGCGATGC CTCCTCACTC 
AGCATCGAGG AGACTAACAA ACTCCGGGCA AAGTTGGGGC TGAAACCCTT GGAGGTTAAT 
GCCATCAAGA AGGAGGCGGG CACCAAGGAG GAGCCCGTGA CAGCTGATGT CATCAACCCT 
ATGGCCTTGC GACAGCGAGA GGAGCTGCGG GAGAAGCTGG CGGCTGCCAA GGAGAAGCGC 
CTGCTGAACC AAAAGCTGGG GAAGATAAAG ACCCTAGGAG AGGATGACCC CTGGCTGGAC 
GACACTGCAG CCTGGATCGA GAGGAGCCGG CAGCTGCAGA AGGAGAAGGA CCTGGCAGAG 
AAGAGGGCCA AGTTACTGGA GGAGATGGAC CAAGAGTTTG GTGTCAGCAC TCTGGTGGAG 
GAGGAGTTCG GGCAGAGGCG GCAGGACCTG TACAGTGCCC GGGACCTGCA GGGCCTCACC 
GTGGAGCATG CCATTGATTC CTTCCGAGAA GGGGAGACAA TGATTCTTAC CCTCAAGGAC 
AAAGGCGTGC TGCAGGAGGA GGAGGACGTG CTGGTGAACG TGAACCTGGT GGATAAGGAG 
CGGGCAGAGA AAAATGTGGA GCTGCGGAAG AAGAAGCCTG ACTACCTGCC CTATGCCGAG 
GACGAGAGCG TGGACGACCT GGCGCAGCAA AAACCTCGCT CTATCCTGTC CAAGTATGAC 
GAAGAGCTTG AAGGGGAGCG GCCACATTCC TTCCGCTTGG AGCAGGGCGG CACGGCTGAT 
GGCCTGCGGG AGCGGGAGCT GGAGGAGATC CGGGCCAAGC TGCGGCTGCA GGCTCAGTCC 
CTGAGCACAG TGGGGCCCCG GCTGGCCTCC GAATACCTCA CGCCTGAGGA GATGGTGACC 
TTTAAAAAGA CCAAGCGGAG GGTGAAGAAA ATCCGCAAGA AGGAGAAGGA GGTAGTAGTG 
CGGGCAGATG ACTTGCTGCC TCTCGGGGAC CAGACTCAGG ATGGGGACTT TGGTTCCAGA 
CTGCGGGGAC GGGGTCGCCG CCGAGTGTCC GAAGTGGAGG AGGAGAAGGA GCCTGTGCCT 
CAGCCCCTGC CGTCGGACGA CACCCGAGTG GAGAACATGG ACATCAGTGA TGAGGAGGAA 
GGTGGAGCTC CACCGCCGGG GTCCCCGCAG GTGCTGGAGG AGGACGAGGC GGAGCTGGAG 
CTGCAGAAGC AGCTGGAGAA GGGACGCCGG CTGCGACAGT TACAGCAGCT ACAGCAGCTG 
CGAGACAGTG GCGAGAAGGT GGTGGAGATT GTGAAGAAGC TGGAGTCTCG CCAGCGGGGC 
TGGGAGGAGG ATGAGGATCC CGAGCGGAAG GGGGCCATCG TGTTCAACGC CACGTCCGAG 
TTCTGCCGCA CCTTGGGGGA GATCCCCACC TACGGGCTGG CTGGCAATCG CGAGGAGCAG 
GAGGAGCTCA TGGACTTTGA ACGGGATGAG GAGCGCTCAG CCAACGGTGG CTCCGAATCT 
GACGGGGAGG AGAACATCGG CTGGAGCACG GTGAACCTGG ACGAGGAGAA GCAGCAGCAG 
GATTTCTCTG CTTCCTCCAC CACCATCCTG GACGAGGAAC CGATCGTGAA TAGGGGGCTG 
GCAGCTGCCC TGCTCCTGTG TCAGAACAAA GGGCTGCTGG AGACCACAGT GCAGAAGGTG 
GCCCGGGTGA AGGCCCCCAA CAAGTCGCTG CCCTCAGCCG TGTACTGCAT CGAGGATAAG 
ATGGCCATCG ATGACAAGTA CAGCCGGAGG GAGGAATACC GAGGCTTCAC ACAGGACTTC 
AAGGAGAAGG ACGGCTACAA ACCCGACGTT AAGATCGAAT ACGTGGATGA GACGGGCCGG 
AAACTCACAC CCAAGGAGGC TTTCCGGCAG CTGTCGCACC GCTTCCATGG CAAGGGCTCA 
GGCAAGATGA AGACAGAGCG GCGGATGAAG AAGCTGGACG AGGAGGCGCT CCTGAAGAAG 
ATGAGCTCCA GCGACACGCC CCTGGGCACC GTGGCCCTGC TCCAGGAGAA GCAGAAGGCT 
CAGAAGACCC CCTACATCGT GCTCAGCGGC AGCGGCAAGA GCATGAACGC GAACACCATC 
ACCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM Structure of a Pre-catalytic Human Spliceosome Primed for Activation.
Cell170(4)701-713.e11(2017 Aug)
Bertram K,Agafonov DE,Dybkov O,Haselbach D,Leelaram MN,Will CL,Urlaub H,Kastner B,L?hrmann R,Stark H

Mutant versions of von Hippel-Lindau (VHL) can protect HIF1? from SART1-mediated degradation in clear-cell renal cell carcinoma.
Oncogene35(5)587-94(2016 Feb)
Ord??ez-Navadijo ?,Fuertes-Yebra E,Acosta-Iborra B,Balsa E,Elorza A,Aragon?s J,Landazuri MO

Expression of Interferon Effector Gene SART1 Correlates with Interferon Treatment Response against Hepatitis B Infection.
Mediators of inflammation20163894816(2016)
Li Y,Zhu C,Wang F,Zhu T,Li J,Liu S,Xiao F

The spliceosome factor SART1 exerts its anti-HCV action through mRNA splicing.
Journal of hepatology62(5)1024-32(2015 May)
Lin W,Zhu C,Hong J,Zhao L,Jilg N,Fusco DN,Schaefer EA,Brisac C,Liu X,Peng LF,Xu Q,Chung RT

UBL5 is essential for pre-mRNA splicing and sister chromatid cohesion in human cells.
EMBO reports15(9)956-64(2014 Sep)
Oka Y,Varmark H,Vitting-Seerup K,Beli P,Waage J,Hakobyan A,Mistrik M,Choudhary C,Rohde M,Bekker-Jensen S,Mailand N

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SART1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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