CLDN12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLDN12
Entrez Gene ID	9069
Full Name	claudin 12
General protein information	Preferred Names claudin 12 Names claudin-12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q21.13
Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is expressed in the inner ear and bladder epithelium, and it is over-expressed in colorectal carcinomas. This protein and claudin 2 are critical for vitamin D-dependent Ca2+ absorption between enterocytes. Multiple alternatively spliced transcript variants encoding the same protein have been found.[provided by RefSeq, Sep 2011].
Disorder MIM:	611232

mRNA and Protein(s)

mRNA	Protein	Name
NM_001185073.2	NP_001172002.1	claudin-12
NM_001185072.2	NP_001172001.1	claudin-12
NM_012129.5	NP_036261.1	claudin-12
NM_001185073.3	NP_001172002.1	claudin-12
NM_012129.4	NP_036261.1	claudin-12
NM_001185072.3	NP_001172001.1	claudin-12

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CLDN12	NP_001069591.1
Danio rerio (zebrafish)	cldn12	NP_571848.1
Gallus gallus (chicken)	CLDN12	XP_004939337.1
	CLDN12	XP_001092396.2
Homo sapiens (human)	CLDN12	NP_001172001.1
Pan troglodytes (chimpanzee)	CLDN12	XP_003339455.1
Canis lupus familiaris (dog)	CLDN12	XP_005628548.1
Rattus norvegicus (Norway rat)	Cldn12	NP_001094283.1
Mus musculus (house mouse)	Cldn12	NP_001180589.1
Xenopus tropicalis (tropical clawed frog)	cldn12	NP_001016851.1

Gene Ontology
Bibliography

Related articles in PubMed

Prognostic significance of Claudin 12 in estrogen receptor-negative breast cancer.
Iravani O, Yip GW, Thike AA, Chua PJ, Jane Scully O, Tan PH, Bay BH
Journal of clinical pathology69(10)878-83(2016 Oct)

Interleukin-18 enhances breast cancer cell migration via down-regulation of claudin-12 and induction of the p38 MAPK pathway.
Yang Y, Cheon S, Jung MK, Song SB, Kim D, Kim HJ, Park H, Bang SI, Cho D
Biochemical and biophysical research communications459(3)379-86(2015 Apr)

Claudin association with CD81 defines hepatitis C virus entry.
Harris HJ, Davis C, Mullins JG, Hu K, Goodall M, Farquhar MJ, Mee CJ, McCaffrey K, Young S, Drummer H, Balfe P, McKeating JA
The Journal of biological chemistry285(27)21092-102(2010 Jul)

Claudins in human cancer: a review.
Ouban A, Ahmed AA
Histology and histopathology25(1)83-90(2010 01)

The claudins.
Lal-Nag M, Morin PJ
Genome biology10(8)235(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CLDN 12 expression could be clinically useful for predicting the survival of the estrogen receptor (ER)-negative subgroup of patients with breast cancer.
Title: Prognostic significance of Claudin 12 in estrogen receptor-negative breast cancer.

Knockdown of claudin-3, claudin-4, and claudin-12, but not claudin-1, increased breast cancer MCF-7 cell migration with maximal effects observed in claudin-12 siRNA-transfected cells.
Title: Interleukin-18 enhances breast cancer cell migration via down-regulation of claudin-12 and induction of the p38 MAPK pathway.

These findings strongly suggest that claudin-2- and/or claudin-12-based tight junctions form paracellular Ca(2+) channels in intestinal epithelia, and they highlight a novel mechanism behind vitamin D-dependent calcium homeostasis.
Title: Tight junction proteins claudin-2 and -12 are critical for vitamin D-dependent Ca2+ absorption between enterocytes.

Differential expression of genes encoding claudins in colorectal cancer suggests that these tight junction proteins may be associated to and involved in tumorigenesis.
Title: Differential expression of genes encoding tight junction proteins in colorectal cancer: frequent dysregulation of claudin-1, -8 and -12.

The following CLDN12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLDN12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04869	NM_012129.5 Latest version!	Homo sapiens claudin 12 (CLDN12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04869	NM_001185072.3 Latest version!	Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04869	NM_001185073.3 Latest version!	Homo sapiens claudin 12 (CLDN12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu04869	NM_001185073.2	Homo sapiens claudin 12 (CLDN12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04869	NM_012129.4	Homo sapiens claudin 12 (CLDN12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04869	NM_001185072.2	Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLDN12 ( NM_001185073.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172002.1 Homo sapiens claudin 12 (CLDN12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04869
Clone ID Related Accession (Same CDS sequence)	NM_001185073.2 , NM_001185072.2 , NM_012129.5 , NM_001185073.3 , NM_012129.4 , NM_001185072.3
Accession Version	NM_001185073.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	claudin-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062973.1, DB224591.1, AK312480.1, BC036754.2, BC068532.1 and AL136770.1. On Dec 9, 2010 this sequence version replaced NM_001185073.1. Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in the 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.231538.1, SRR1803613.115671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172002.1
CDS	344..1078
Translation	MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALLLCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIHLNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Target ORF information:

RefSeq Version	NM_001185073.2
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 12 (CLDN12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185073.2

ORF Insert Sequence:

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLDN12 ( NM_001185072.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172001.1 Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04869
Clone ID Related Accession (Same CDS sequence)	NM_001185073.2 , NM_001185072.2 , NM_012129.5 , NM_001185073.3 , NM_012129.4 , NM_001185072.3
Accession Version	NM_001185072.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	claudin-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062973.1, AK312480.1, BC036754.2, BC068532.1 and AL136770.1. On Dec 9, 2010 this sequence version replaced NM_001185072.1. Transcript Variant: This variant (1) is the longest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.77004.1, SRR1660807.79304.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172001.1
CDS	383..1117
Translation	MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALLLCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIHLNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Target ORF information:

RefSeq Version	NM_001185072.2
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185072.2

ORF Insert Sequence:

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLDN12 ( NM_012129.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_036261.1 Homo sapiens claudin 12 (CLDN12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu04869
Clone ID Related Accession (Same CDS sequence)	NM_001185073.2 , NM_001185072.2 , NM_012129.5 , NM_001185073.3 , NM_012129.4 , NM_001185072.3
Accession Version	NM_012129.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	claudin-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006153.2. On May 31, 2019 this sequence version replaced NM_012129.4. Transcript Variant: This variant (3) lacks an exon in the 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1967800.1, BC068532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036261.1
CDS	213..947
Translation	MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALLLCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIHLNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Target ORF information:

RefSeq Version	NM_012129.5
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 12 (CLDN12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012129.5

ORF Insert Sequence:

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLDN12 ( NM_001185073.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172002.1 Homo sapiens claudin 12 (CLDN12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04869
Clone ID Related Accession (Same CDS sequence)	NM_001185073.2 , NM_001185072.2 , NM_012129.5 , NM_001185073.3 , NM_012129.4 , NM_001185072.3
Accession Version	NM_001185073.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	claudin-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006153.2. On May 31, 2019 this sequence version replaced NM_001185073.2. Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in the 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.115671.1, SRR1660803.240223.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172002.1
CDS	217..951
Translation	MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALLLCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIHLNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Target ORF information:

RefSeq Version	NM_001185073.3
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 12 (CLDN12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185073.3

ORF Insert Sequence:

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLDN12 ( NM_012129.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036261.1 Homo sapiens claudin 12 (CLDN12), transcript variant 3, mRNA.

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CloneID	OHu04869
Clone ID Related Accession (Same CDS sequence)	NM_001185073.2 , NM_001185072.2 , NM_012129.5 , NM_001185073.3 , NM_012129.4 , NM_001185072.3
Accession Version	NM_012129.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	claudin-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB062973.1, BC068532.1, BC036754.2 and AL136770.1. On Dec 10, 2010 this sequence version replaced NM_012129.3. Transcript Variant: This variant (3) lacks an exon in the 5' UTR, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC068532.1, SRR1660807.117964.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036261.1
CDS	340..1074
Translation	MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALLLCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIHLNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Target ORF information:

RefSeq Version	NM_012129.4
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 12 (CLDN12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012129.4

ORF Insert Sequence:

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CLDN12 ( NM_001185072.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172001.1 Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.

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CloneID	OHu04869
Clone ID Related Accession (Same CDS sequence)	NM_001185073.2 , NM_001185072.2 , NM_012129.5 , NM_001185073.3 , NM_012129.4 , NM_001185072.3
Accession Version	NM_001185072.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	claudin-12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006153.2. On Nov 24, 2018 this sequence version replaced NM_001185072.2. Transcript Variant: This variant (1) is the longest transcript. Variants 1-3 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.253211.1, SRR1660809.15320.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000496677.6/ ENSP00000419053.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172001.1
CDS	256..990
Translation	MGCRDVHAATVLSFLCGIASVAGLFAGTLLPNWRKLRLITFNRNEKNLTVYTGLWVKCARYDGSSDCLMYDTTWYSSVDQLDLRVLQFALPLSMLIAMGALLLCLIGMCNTAFRSSVPNIKLAKCLVNSAGCHLVAGLLFFLAGTVSLSPSIWVIFYNIHLNKKFEPVFSFDYAVYVTIASAGGLFMTSLILFIWYCTCKSLPSPFWQPLYSHPPSMHTYSQPYSARSRLSAIEIDIPVVSHTT

Target ORF information:

RefSeq Version	NM_001185072.3
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 12 (CLDN12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185072.3

ORF Insert Sequence:

ATGGGCTGTC GGGATGTCCA CGCAGCCACA GTCCTTTCCT TCCTGTGTGG AATCGCCTCA 
GTAGCAGGCC TCTTTGCAGG GACTCTGCTT CCCAACTGGA GAAAATTACG ATTGATCACA 
TTCAACAGAA ACGAGAAGAA CCTGACTGTT TACACAGGCC TGTGGGTGAA ATGTGCCCGG 
TATGACGGGA GCAGTGACTG CCTGATGTAC GACACTACTT GGTACTCATC AGTTGACCAG 
CTGGACCTGC GTGTCCTCCA GTTTGCCCTA CCCCTCAGCA TGCTGATCGC CATGGGTGCC 
CTGCTGCTCT GCCTGATTGG AATGTGCAAC ACTGCCTTCA GGTCCTCGGT GCCCAACATC 
AAACTGGCCA AGTGTCTGGT CAATAGTGCA GGTTGCCACC TGGTGGCTGG GCTGCTATTT 
TTCCTGGCAG GTACTGTGAG CCTCTCCCCA TCTATCTGGG TCATCTTTTA TAACATCCAT 
CTGAACAAGA AGTTTGAGCC AGTCTTTTCA TTTGACTATG CAGTGTATGT CACTATTGCT 
AGTGCTGGGG GCCTGTTTAT GACTTCCCTT ATACTATTTA TTTGGTATTG TACATGCAAA 
TCTTTGCCTT CTCCTTTCTG GCAACCATTG TACTCCCATC CACCCAGTAT GCATACTTAC 
TCACAGCCCT ATTCAGCACG CTCTCGCCTC TCTGCCATTG AAATTGACAT TCCAGTAGTT 
TCACACACCA CTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prognostic significance of Claudin 12 in estrogen receptor-negative breast cancer.
Journal of clinical pathology69(10)878-83(2016 Oct)
Iravani O,Yip GW,Thike AA,Chua PJ,Jane Scully O,Tan PH,Bay BH

Interleukin-18 enhances breast cancer cell migration via down-regulation of claudin-12 and induction of the p38 MAPK pathway.
Biochemical and biophysical research communications459(3)379-86(2015 Apr)
Yang Y,Cheon S,Jung MK,Song SB,Kim D,Kim HJ,Park H,Bang SI,Cho D

Claudin association with CD81 defines hepatitis C virus entry.
The Journal of biological chemistry285(27)21092-102(2010 Jul)
Harris HJ,Davis C,Mullins JG,Hu K,Goodall M,Farquhar MJ,Mee CJ,McCaffrey K,Young S,Drummer H,Balfe P,McKeating JA

Claudins in human cancer: a review.
Histology and histopathology25(1)83-90(2010 01)
Ouban A,Ahmed AA

The claudins.
Genome biology10(8)235(2009)
Lal-Nag M,Morin PJ

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CLDN12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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