PAPSS1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PAPSS1
Entrez Gene ID	9061
Full Name	3'-phosphoadenosine 5'-phosphosulfate synthase 1
Synonyms	ATPSK1,PAPSS,SK1
General protein information	Preferred Names 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Names bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 3-prime-phosphoadenosine 5-prime-phosphosulfate synthase 1 PAPS synthase 1 PAPSS 1 SK 1 adenylyl-sulfate kinase sulfate adenylyltransferase sulfurylase kinase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4q25
Summary	Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes (Xu et al., 2000 [PubMed 10679223]). SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (MIM 603005).[supplied by OMIM, Mar 2008].
Disorder MIM:	603262

mRNA and Protein(s)

mRNA	Protein	Name
NM_005443.4	NP_005434.4	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
NM_005443.5	NP_005434.4	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
XM_011532400.2	XP_011530702.1	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 isoform X1
XM_011532400.1	XP_011530702.1	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 isoform X1
XM_011532401.1	XP_011530703.1	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PAPSS1	XP_517384.4
Saccharomyces cerevisiae (baker's yeast)	MET14	NP_012925.3
Magnaporthe oryzae (rice blast fungus)	MGG_06348	XP_003717233.1
Drosophila melanogaster (fruit fly)	Papss	NP_001262072.1
Neurospora crassa	NCU09896	XP_958621.1
Homo sapiens (human)	PAPSS1	NP_005434.4
Rattus norvegicus (Norway rat)	Papss1	NP_001099941.1
Kluyveromyces lactis	KLLA0B13321g	XP_452124.1
Schizosaccharomyces pombe (fission yeast)	met14	NP_594718.1
Xenopus tropicalis (tropical clawed frog)	papss1	NP_001006743.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001256	XP_003435789.1
	PAPSS1	XP_001085840.2
Canis lupus familiaris (dog)	PAPSS1	XP_851070.2
Bos taurus (cattle)	PAPSS1	NP_001029382.1
Mus musculus (house mouse)	Papss1	NP_035993.1
Gallus gallus (chicken)	PAPSS1	XP_420493.3
Danio rerio (zebrafish)	papss1	NP_001122171.1

Gene Ontology
Bibliography

Related articles in PubMed

Leung AWY, Veinotte CJ, Melong N, Oh MH, Chen K, Enfield KSS, Backstrom I, Warburton C, Yapp D, Berman JN, Bally MB, Lockwood WW
Clinical cancer research : an official journal of the American Association for Cancer Research23(21)6555-6566(2017 Nov)

CIB1 contributes to oncogenic signalling by Ras via modulating the subcellular localisation of sphingosine kinase 1.
Zhu W, Gliddon BL, Jarman KE, Moretti PAB, Tin T, Parise LV, Woodcock JM, Powell JA, Ruszkiewicz A, Pitman MR, Pitson SM
Oncogene36(18)2619-2627(2017 May)

Upregulation of UGT2B4 Expression by 3'-Phosphoadenosine-5'-Phosphosulfate Synthase Knockdown: Implications for Coordinated Control of Bile Acid Conjugation.
Barrett KG, Fang H, Cukovic D, Dombkowski AA, Kocarek TA, Runge-Morris M
Drug metabolism and disposition: the biological fate of chemicals43(7)1061-70(2015 Jul)

Leptin induces upregulation of sphingosine kinase 1 in oestrogen receptor-negative breast cancer via Src family kinase-mediated, janus kinase 2-independent pathway.
Alshaker H, Krell J, Frampton AE, Waxman J, Blyuss O, Zaikin A, Winkler M, Stebbing J, Yag?e E, Pchejetski D
Breast cancer research : BCR16(5)426(2014 Oct)

Sphingosine kinase 1 and cancer: a systematic review and meta-analysis.
Zhang Y, Wang Y, Wan Z, Liu S, Cao Y, Zeng Z
PloS one9(2)e90362(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we found that ectopic expression of oncogenic KRas and HRas in cells resulted in elevated CIB1 expression. We previously described the Ca(2+)-myristoyl switch function of CIB1, and its ability to facilitate agonist-induced plasma membrane localisation of sphingosine kinase 1 (SK1), a location where SK1 is known to elicit oncogenic signalling.
Title: CIB1 contributes to oncogenic signalling by Ras via modulating the subcellular localisation of sphingosine kinase 1.

These data indicate that knocking down PAPSS increases UGT2B4 transcription and mRNA stability as a compensatory response to the loss of SULT2A1 activity
Title: Upregulation of UGT2B4 Expression by 3'-Phosphoadenosine-5'-Phosphosulfate Synthase Knockdown: Implications for Coordinated Control of Bile Acid Conjugation.

leptin signalling links a novel SFK/ERK1/2-mediated pathway and SK1 expression.
Title: Leptin induces upregulation of sphingosine kinase 1 in oestrogen receptor-negative breast cancer via Src family kinase-mediated, janus kinase 2-independent pathway.

SK1 positivity and high expression were significantly associated with cancer and a shorter 5-year and overall survival.
Title: Sphingosine kinase 1 and cancer: a systematic review and meta-analysis.

study showed expression patterns of SULT1E1 and PAPSS in breast and endometrial tissues; the estrogen sulfation enzymes were comparatively higher in the tumorous tissues than adjacent normal tissues; overexpression of SULT1E1 and PAPSS1 retarded MCF-7 cells growth in vivo and in vitro by arresting cell cycles and inducing apoptosis
Title: Effect of estrogen sulfation by SULT1E1 and PAPSS on the development of estrogen-dependent cancers.

The following PAPSS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PAPSS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu62439	XM_011532400.2 Latest version!	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu62439	XM_011532401.1 Latest version!	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu13190	NM_005443.5 Latest version!	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu13190	NM_005443.4	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu62439	XM_011532400.1	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PAPSS1 ( XM_011532400.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530702.1 Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu62439
Clone ID Related Accession (Same CDS sequence)	XM_011532400.2 , XM_011532400.1 , XM_011532401.1
Accession Version	XM_011532400.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011532400.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG CAACCAATGT CACCTACCAA GCCCATCATG TCAGCAGGAA CAAGAGAGGT 
CAGGTGGTGG GGACCAGAGG TGGCTTTCGT GGTTGCACAG TTTGGCTAAC AGGCTTGTCT 
GGAGCGGGAA AGACTACTGT GAGCATGGCC TTGGAGGAGT ACCTGGTTTG TCATGGTATT 
CCATGCTACA CTCTGGATGG TGACAATATT CGTCAAGGTC TCAATAAAAA TCTTGGCTTT 
AGTCCTGAAG ACAGAGAAGA GAATGTTCGA CGCATCGCAG AAGTTGCTAA ACTGTTTGCA 
GATGCTGGCT TAGTGTGCAT CACAAGTTTC ATATCACCTT ACACTCAGGA TCGCAACAAT 
GCAAGGCAAA TTCATGAAGG TGCAAGTTTA CCGTTTTTTG AAGTATTTGT TGATGCTCCT 
CTGCATGTTT GTGAACAGAG GGATGTCAAA GGACTCTACA AAAAAGCCCG GGCAGGAGAA 
ATTAAAGGTT TCACTGGGAT CGATTCTGAA TATGAAAAGC CAGAGGCCCC TGAGTTGGTG 
CTGAAAACAG ACTCCTGTGA TGTAAATGAC TGTGTCCAGC AAGTTGTGGA ACTTCTACAG 
GAACGGGATA TTGTACCTGT GGATGCATCT TATGAAGTAA AAGAACTATA TGTGCCAGAA 
AATAAACTTC ATTTGGCAAA AACAGATGCG GAAACATTAC CAGCACTGAA AATTAATAAA 
GTGGATATGC AGTGGGTGCA GGTTTTGGCA GAAGGTTGGG CAACCCCATT GAATGGCTTT 
ATGAGAGAGA GGGAGTACTT GCAGTGCCTT CATTTTGATT GTCTTCTGGA TGGAGGTGTC 
ATTAACTTGT CAGTACCTAT AGTTCTGACT GCGACTCATG AAGATAAAGA GAGGCTGGAC 
GGCTGTACAG CATTTGCTCT GATGTATGAG GGCCGCCGTG TGGCCATTCT TCGCAATCCA 
GAGTTTTTTG AGCACAGGAA AGAGGAGCGC TGTGCCAGAC AGTGGGGAAC GACATGCAAG 
AACCACCCCT ATATTAAGAT GGTGATGGAA CAAGGAGATT GGCTGATTGG AGGAGATCTT 
CAAGTCTTGG ATCGAGTTTA TTGGAATGAT GGTCTTGATC AGTATCGTCT TACTCCTACT 
GAGCTAAAGC AGAAATTTAA AGATATGAAT GCTGATGCTG TCTTTGCATT TCAACTACGC 
AACCCAGTGC ACAATGGACA TGCCCTGTTA ATGCAGGATA CCCATAAGCA ACTTCTAGAG 
AGGGGCTACC GGCGCCCTGT CCTCCTCCTC CACCCTCTGG GTGGCTGGAC AAAGGATGAC 
GATGTTCCTT TGATGTGGCG TATGAAGCAG CATGCTGCAG TGTTGGAGGA AGGAGTTCTG 
AATCCTGAGA CGACAGTGGT GGCCATCTTC CCATCTCCCA TGATGTATGC TGGACCAACT 
GAGGTCCAGT GGCATTGCAG AGCACGGATG GTTGCAGGAG CCAACTTTTA CATTGTTGGA 
CGAGACCCTG CTGGCATGCC TCATCCAGAA ACAGGGAAGG ATCTTTATGA GCCAAGTCAT 
GGTGCCAAAG TGCTGACGAT GGCCCCTGGT TTAATCACTT TGGAAATAGT TCCCTTTCGA 
GTTGCAGCTT ACAACAAGAA AAAGAAGCGT ATGGACTACT ATGACTCTGA ACACCATGAA 
GACTTTGAAT TTATTTCAGG AACACGAATG CGCAAACTTG CTCGAGAAGG CCAGAAACCA 
CCTGAAGGTT TCATGGCTCC CAAGGCTTGG ACCGTGCTGA CAGAATACTA CAAATCCTTG 
GAGAAAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530702.1
CDS	185..1996
Translation	MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

Target ORF information:

RefSeq Version	XM_011532400.2
Organism	Homo sapiens(human)
Definition	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532400.2

ORF Insert Sequence:

ATGCAGAGAG CAACCAATGT CACCTACCAA GCCCATCATG TCAGCAGGAA CAAGAGAGGT 
CAGGTGGTGG GGACCAGAGG TGGCTTTCGT GGTTGCACAG TTTGGCTAAC AGGCTTGTCT 
GGAGCGGGAA AGACTACTGT GAGCATGGCC TTGGAGGAGT ACCTGGTTTG TCATGGTATT 
CCATGCTACA CTCTGGATGG TGACAATATT CGTCAAGGTC TCAATAAAAA TCTTGGCTTT 
AGTCCTGAAG ACAGAGAAGA GAATGTTCGA CGCATCGCAG AAGTTGCTAA ACTGTTTGCA 
GATGCTGGCT TAGTGTGCAT CACAAGTTTC ATATCACCTT ACACTCAGGA TCGCAACAAT 
GCAAGGCAAA TTCATGAAGG TGCAAGTTTA CCGTTTTTTG AAGTATTTGT TGATGCTCCT 
CTGCATGTTT GTGAACAGAG GGATGTCAAA GGACTCTACA AAAAAGCCCG GGCAGGAGAA 
ATTAAAGGTT TCACTGGGAT CGATTCTGAA TATGAAAAGC CAGAGGCCCC TGAGTTGGTG 
CTGAAAACAG ACTCCTGTGA TGTAAATGAC TGTGTCCAGC AAGTTGTGGA ACTTCTACAG 
GAACGGGATA TTGTACCTGT GGATGCATCT TATGAAGTAA AAGAACTATA TGTGCCAGAA 
AATAAACTTC ATTTGGCAAA AACAGATGCG GAAACATTAC CAGCACTGAA AATTAATAAA 
GTGGATATGC AGTGGGTGCA GGTTTTGGCA GAAGGTTGGG CAACCCCATT GAATGGCTTT 
ATGAGAGAGA GGGAGTACTT GCAGTGCCTT CATTTTGATT GTCTTCTGGA TGGAGGTGTC 
ATTAACTTGT CAGTACCTAT AGTTCTGACT GCGACTCATG AAGATAAAGA GAGGCTGGAC 
GGCTGTACAG CATTTGCTCT GATGTATGAG GGCCGCCGTG TGGCCATTCT TCGCAATCCA 
GAGTTTTTTG AGCACAGGAA AGAGGAGCGC TGTGCCAGAC AGTGGGGAAC GACATGCAAG 
AACCACCCCT ATATTAAGAT GGTGATGGAA CAAGGAGATT GGCTGATTGG AGGAGATCTT 
CAAGTCTTGG ATCGAGTTTA TTGGAATGAT GGTCTTGATC AGTATCGTCT TACTCCTACT 
GAGCTAAAGC AGAAATTTAA AGATATGAAT GCTGATGCTG TCTTTGCATT TCAACTACGC 
AACCCAGTGC ACAATGGACA TGCCCTGTTA ATGCAGGATA CCCATAAGCA ACTTCTAGAG 
AGGGGCTACC GGCGCCCTGT CCTCCTCCTC CACCCTCTGG GTGGCTGGAC AAAGGATGAC 
GATGTTCCTT TGATGTGGCG TATGAAGCAG CATGCTGCAG TGTTGGAGGA AGGAGTTCTG 
AATCCTGAGA CGACAGTGGT GGCCATCTTC CCATCTCCCA TGATGTATGC TGGACCAACT 
GAGGTCCAGT GGCATTGCAG AGCACGGATG GTTGCAGGAG CCAACTTTTA CATTGTTGGA 
CGAGACCCTG CTGGCATGCC TCATCCAGAA ACAGGGAAGG ATCTTTATGA GCCAAGTCAT 
GGTGCCAAAG TGCTGACGAT GGCCCCTGGT TTAATCACTT TGGAAATAGT TCCCTTTCGA 
GTTGCAGCTT ACAACAAGAA AAAGAAGCGT ATGGACTACT ATGACTCTGA ACACCATGAA 
GACTTTGAAT TTATTTCAGG AACACGAATG CGCAAACTTG CTCGAGAAGG CCAGAAACCA 
CCTGAAGGTT TCATGGCTCC CAAGGCTTGG ACCGTGCTGA CAGAATACTA CAAATCCTTG 
GAGAAAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPSS1 ( XM_011532400.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530702.1 Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu62439
Clone ID Related Accession (Same CDS sequence)	XM_011532400.2 , XM_011532400.1 , XM_011532401.1
Accession Version	XM_011532400.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG CAACCAATGT CACCTACCAA GCCCATCATG TCAGCAGGAA CAAGAGAGGT 
CAGGTGGTGG GGACCAGAGG TGGCTTTCGT GGTTGCACAG TTTGGCTAAC AGGCTTGTCT 
GGAGCGGGAA AGACTACTGT GAGCATGGCC TTGGAGGAGT ACCTGGTTTG TCATGGTATT 
CCATGCTACA CTCTGGATGG TGACAATATT CGTCAAGGTC TCAATAAAAA TCTTGGCTTT 
AGTCCTGAAG ACAGAGAAGA GAATGTTCGA CGCATCGCAG AAGTTGCTAA ACTGTTTGCA 
GATGCTGGCT TAGTGTGCAT CACAAGTTTC ATATCACCTT ACACTCAGGA TCGCAACAAT 
GCAAGGCAAA TTCATGAAGG TGCAAGTTTA CCGTTTTTTG AAGTATTTGT TGATGCTCCT 
CTGCATGTTT GTGAACAGAG GGATGTCAAA GGACTCTACA AAAAAGCCCG GGCAGGAGAA 
ATTAAAGGTT TCACTGGGAT CGATTCTGAA TATGAAAAGC CAGAGGCCCC TGAGTTGGTG 
CTGAAAACAG ACTCCTGTGA TGTAAATGAC TGTGTCCAGC AAGTTGTGGA ACTTCTACAG 
GAACGGGATA TTGTACCTGT GGATGCATCT TATGAAGTAA AAGAACTATA TGTGCCAGAA 
AATAAACTTC ATTTGGCAAA AACAGATGCG GAAACATTAC CAGCACTGAA AATTAATAAA 
GTGGATATGC AGTGGGTGCA GGTTTTGGCA GAAGGTTGGG CAACCCCATT GAATGGCTTT 
ATGAGAGAGA GGGAGTACTT GCAGTGCCTT CATTTTGATT GTCTTCTGGA TGGAGGTGTC 
ATTAACTTGT CAGTACCTAT AGTTCTGACT GCGACTCATG AAGATAAAGA GAGGCTGGAC 
GGCTGTACAG CATTTGCTCT GATGTATGAG GGCCGCCGTG TGGCCATTCT TCGCAATCCA 
GAGTTTTTTG AGCACAGGAA AGAGGAGCGC TGTGCCAGAC AGTGGGGAAC GACATGCAAG 
AACCACCCCT ATATTAAGAT GGTGATGGAA CAAGGAGATT GGCTGATTGG AGGAGATCTT 
CAAGTCTTGG ATCGAGTTTA TTGGAATGAT GGTCTTGATC AGTATCGTCT TACTCCTACT 
GAGCTAAAGC AGAAATTTAA AGATATGAAT GCTGATGCTG TCTTTGCATT TCAACTACGC 
AACCCAGTGC ACAATGGACA TGCCCTGTTA ATGCAGGATA CCCATAAGCA ACTTCTAGAG 
AGGGGCTACC GGCGCCCTGT CCTCCTCCTC CACCCTCTGG GTGGCTGGAC AAAGGATGAC 
GATGTTCCTT TGATGTGGCG TATGAAGCAG CATGCTGCAG TGTTGGAGGA AGGAGTTCTG 
AATCCTGAGA CGACAGTGGT GGCCATCTTC CCATCTCCCA TGATGTATGC TGGACCAACT 
GAGGTCCAGT GGCATTGCAG AGCACGGATG GTTGCAGGAG CCAACTTTTA CATTGTTGGA 
CGAGACCCTG CTGGCATGCC TCATCCAGAA ACAGGGAAGG ATCTTTATGA GCCAAGTCAT 
GGTGCCAAAG TGCTGACGAT GGCCCCTGGT TTAATCACTT TGGAAATAGT TCCCTTTCGA 
GTTGCAGCTT ACAACAAGAA AAAGAAGCGT ATGGACTACT ATGACTCTGA ACACCATGAA 
GACTTTGAAT TTATTTCAGG AACACGAATG CGCAAACTTG CTCGAGAAGG CCAGAAACCA 
CCTGAAGGTT TCATGGCTCC CAAGGCTTGG ACCGTGCTGA CAGAATACTA CAAATCCTTG 
GAGAAAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530702.1
CDS	128..1939
Translation	MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

Target ORF information:

RefSeq Version	XM_011532400.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532400.1

ORF Insert Sequence:

ATGCAGAGAG CAACCAATGT CACCTACCAA GCCCATCATG TCAGCAGGAA CAAGAGAGGT 
CAGGTGGTGG GGACCAGAGG TGGCTTTCGT GGTTGCACAG TTTGGCTAAC AGGCTTGTCT 
GGAGCGGGAA AGACTACTGT GAGCATGGCC TTGGAGGAGT ACCTGGTTTG TCATGGTATT 
CCATGCTACA CTCTGGATGG TGACAATATT CGTCAAGGTC TCAATAAAAA TCTTGGCTTT 
AGTCCTGAAG ACAGAGAAGA GAATGTTCGA CGCATCGCAG AAGTTGCTAA ACTGTTTGCA 
GATGCTGGCT TAGTGTGCAT CACAAGTTTC ATATCACCTT ACACTCAGGA TCGCAACAAT 
GCAAGGCAAA TTCATGAAGG TGCAAGTTTA CCGTTTTTTG AAGTATTTGT TGATGCTCCT 
CTGCATGTTT GTGAACAGAG GGATGTCAAA GGACTCTACA AAAAAGCCCG GGCAGGAGAA 
ATTAAAGGTT TCACTGGGAT CGATTCTGAA TATGAAAAGC CAGAGGCCCC TGAGTTGGTG 
CTGAAAACAG ACTCCTGTGA TGTAAATGAC TGTGTCCAGC AAGTTGTGGA ACTTCTACAG 
GAACGGGATA TTGTACCTGT GGATGCATCT TATGAAGTAA AAGAACTATA TGTGCCAGAA 
AATAAACTTC ATTTGGCAAA AACAGATGCG GAAACATTAC CAGCACTGAA AATTAATAAA 
GTGGATATGC AGTGGGTGCA GGTTTTGGCA GAAGGTTGGG CAACCCCATT GAATGGCTTT 
ATGAGAGAGA GGGAGTACTT GCAGTGCCTT CATTTTGATT GTCTTCTGGA TGGAGGTGTC 
ATTAACTTGT CAGTACCTAT AGTTCTGACT GCGACTCATG AAGATAAAGA GAGGCTGGAC 
GGCTGTACAG CATTTGCTCT GATGTATGAG GGCCGCCGTG TGGCCATTCT TCGCAATCCA 
GAGTTTTTTG AGCACAGGAA AGAGGAGCGC TGTGCCAGAC AGTGGGGAAC GACATGCAAG 
AACCACCCCT ATATTAAGAT GGTGATGGAA CAAGGAGATT GGCTGATTGG AGGAGATCTT 
CAAGTCTTGG ATCGAGTTTA TTGGAATGAT GGTCTTGATC AGTATCGTCT TACTCCTACT 
GAGCTAAAGC AGAAATTTAA AGATATGAAT GCTGATGCTG TCTTTGCATT TCAACTACGC 
AACCCAGTGC ACAATGGACA TGCCCTGTTA ATGCAGGATA CCCATAAGCA ACTTCTAGAG 
AGGGGCTACC GGCGCCCTGT CCTCCTCCTC CACCCTCTGG GTGGCTGGAC AAAGGATGAC 
GATGTTCCTT TGATGTGGCG TATGAAGCAG CATGCTGCAG TGTTGGAGGA AGGAGTTCTG 
AATCCTGAGA CGACAGTGGT GGCCATCTTC CCATCTCCCA TGATGTATGC TGGACCAACT 
GAGGTCCAGT GGCATTGCAG AGCACGGATG GTTGCAGGAG CCAACTTTTA CATTGTTGGA 
CGAGACCCTG CTGGCATGCC TCATCCAGAA ACAGGGAAGG ATCTTTATGA GCCAAGTCAT 
GGTGCCAAAG TGCTGACGAT GGCCCCTGGT TTAATCACTT TGGAAATAGT TCCCTTTCGA 
GTTGCAGCTT ACAACAAGAA AAAGAAGCGT ATGGACTACT ATGACTCTGA ACACCATGAA 
GACTTTGAAT TTATTTCAGG AACACGAATG CGCAAACTTG CTCGAGAAGG CCAGAAACCA 
CCTGAAGGTT TCATGGCTCC CAAGGCTTGG ACCGTGCTGA CAGAATACTA CAAATCCTTG 
GAGAAAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPSS1 ( XM_011532401.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011530703.1 Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X1, mRNA.

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CloneID	OHu62439
Clone ID Related Accession (Same CDS sequence)	XM_011532400.2 , XM_011532400.1 , XM_011532401.1
Accession Version	XM_011532401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAGAG CAACCAATGT CACCTACCAA GCCCATCATG TCAGCAGGAA CAAGAGAGGT 
CAGGTGGTGG GGACCAGAGG TGGCTTTCGT GGTTGCACAG TTTGGCTAAC AGGCTTGTCT 
GGAGCGGGAA AGACTACTGT GAGCATGGCC TTGGAGGAGT ACCTGGTTTG TCATGGTATT 
CCATGCTACA CTCTGGATGG TGACAATATT CGTCAAGGTC TCAATAAAAA TCTTGGCTTT 
AGTCCTGAAG ACAGAGAAGA GAATGTTCGA CGCATCGCAG AAGTTGCTAA ACTGTTTGCA 
GATGCTGGCT TAGTGTGCAT CACAAGTTTC ATATCACCTT ACACTCAGGA TCGCAACAAT 
GCAAGGCAAA TTCATGAAGG TGCAAGTTTA CCGTTTTTTG AAGTATTTGT TGATGCTCCT 
CTGCATGTTT GTGAACAGAG GGATGTCAAA GGACTCTACA AAAAAGCCCG GGCAGGAGAA 
ATTAAAGGTT TCACTGGGAT CGATTCTGAA TATGAAAAGC CAGAGGCCCC TGAGTTGGTG 
CTGAAAACAG ACTCCTGTGA TGTAAATGAC TGTGTCCAGC AAGTTGTGGA ACTTCTACAG 
GAACGGGATA TTGTACCTGT GGATGCATCT TATGAAGTAA AAGAACTATA TGTGCCAGAA 
AATAAACTTC ATTTGGCAAA AACAGATGCG GAAACATTAC CAGCACTGAA AATTAATAAA 
GTGGATATGC AGTGGGTGCA GGTTTTGGCA GAAGGTTGGG CAACCCCATT GAATGGCTTT 
ATGAGAGAGA GGGAGTACTT GCAGTGCCTT CATTTTGATT GTCTTCTGGA TGGAGGTGTC 
ATTAACTTGT CAGTACCTAT AGTTCTGACT GCGACTCATG AAGATAAAGA GAGGCTGGAC 
GGCTGTACAG CATTTGCTCT GATGTATGAG GGCCGCCGTG TGGCCATTCT TCGCAATCCA 
GAGTTTTTTG AGCACAGGAA AGAGGAGCGC TGTGCCAGAC AGTGGGGAAC GACATGCAAG 
AACCACCCCT ATATTAAGAT GGTGATGGAA CAAGGAGATT GGCTGATTGG AGGAGATCTT 
CAAGTCTTGG ATCGAGTTTA TTGGAATGAT GGTCTTGATC AGTATCGTCT TACTCCTACT 
GAGCTAAAGC AGAAATTTAA AGATATGAAT GCTGATGCTG TCTTTGCATT TCAACTACGC 
AACCCAGTGC ACAATGGACA TGCCCTGTTA ATGCAGGATA CCCATAAGCA ACTTCTAGAG 
AGGGGCTACC GGCGCCCTGT CCTCCTCCTC CACCCTCTGG GTGGCTGGAC AAAGGATGAC 
GATGTTCCTT TGATGTGGCG TATGAAGCAG CATGCTGCAG TGTTGGAGGA AGGAGTTCTG 
AATCCTGAGA CGACAGTGGT GGCCATCTTC CCATCTCCCA TGATGTATGC TGGACCAACT 
GAGGTCCAGT GGCATTGCAG AGCACGGATG GTTGCAGGAG CCAACTTTTA CATTGTTGGA 
CGAGACCCTG CTGGCATGCC TCATCCAGAA ACAGGGAAGG ATCTTTATGA GCCAAGTCAT 
GGTGCCAAAG TGCTGACGAT GGCCCCTGGT TTAATCACTT TGGAAATAGT TCCCTTTCGA 
GTTGCAGCTT ACAACAAGAA AAAGAAGCGT ATGGACTACT ATGACTCTGA ACACCATGAA 
GACTTTGAAT TTATTTCAGG AACACGAATG CGCAAACTTG CTCGAGAAGG CCAGAAACCA 
CCTGAAGGTT TCATGGCTCC CAAGGCTTGG ACCGTGCTGA CAGAATACTA CAAATCCTTG 
GAGAAAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011530703.1
CDS	214..2025
Translation	MQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

Target ORF information:

RefSeq Version	XM_011532401.1
Organism	Homo sapiens(human)
Definition	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532401.1

ORF Insert Sequence:

ATGCAGAGAG CAACCAATGT CACCTACCAA GCCCATCATG TCAGCAGGAA CAAGAGAGGT 
CAGGTGGTGG GGACCAGAGG TGGCTTTCGT GGTTGCACAG TTTGGCTAAC AGGCTTGTCT 
GGAGCGGGAA AGACTACTGT GAGCATGGCC TTGGAGGAGT ACCTGGTTTG TCATGGTATT 
CCATGCTACA CTCTGGATGG TGACAATATT CGTCAAGGTC TCAATAAAAA TCTTGGCTTT 
AGTCCTGAAG ACAGAGAAGA GAATGTTCGA CGCATCGCAG AAGTTGCTAA ACTGTTTGCA 
GATGCTGGCT TAGTGTGCAT CACAAGTTTC ATATCACCTT ACACTCAGGA TCGCAACAAT 
GCAAGGCAAA TTCATGAAGG TGCAAGTTTA CCGTTTTTTG AAGTATTTGT TGATGCTCCT 
CTGCATGTTT GTGAACAGAG GGATGTCAAA GGACTCTACA AAAAAGCCCG GGCAGGAGAA 
ATTAAAGGTT TCACTGGGAT CGATTCTGAA TATGAAAAGC CAGAGGCCCC TGAGTTGGTG 
CTGAAAACAG ACTCCTGTGA TGTAAATGAC TGTGTCCAGC AAGTTGTGGA ACTTCTACAG 
GAACGGGATA TTGTACCTGT GGATGCATCT TATGAAGTAA AAGAACTATA TGTGCCAGAA 
AATAAACTTC ATTTGGCAAA AACAGATGCG GAAACATTAC CAGCACTGAA AATTAATAAA 
GTGGATATGC AGTGGGTGCA GGTTTTGGCA GAAGGTTGGG CAACCCCATT GAATGGCTTT 
ATGAGAGAGA GGGAGTACTT GCAGTGCCTT CATTTTGATT GTCTTCTGGA TGGAGGTGTC 
ATTAACTTGT CAGTACCTAT AGTTCTGACT GCGACTCATG AAGATAAAGA GAGGCTGGAC 
GGCTGTACAG CATTTGCTCT GATGTATGAG GGCCGCCGTG TGGCCATTCT TCGCAATCCA 
GAGTTTTTTG AGCACAGGAA AGAGGAGCGC TGTGCCAGAC AGTGGGGAAC GACATGCAAG 
AACCACCCCT ATATTAAGAT GGTGATGGAA CAAGGAGATT GGCTGATTGG AGGAGATCTT 
CAAGTCTTGG ATCGAGTTTA TTGGAATGAT GGTCTTGATC AGTATCGTCT TACTCCTACT 
GAGCTAAAGC AGAAATTTAA AGATATGAAT GCTGATGCTG TCTTTGCATT TCAACTACGC 
AACCCAGTGC ACAATGGACA TGCCCTGTTA ATGCAGGATA CCCATAAGCA ACTTCTAGAG 
AGGGGCTACC GGCGCCCTGT CCTCCTCCTC CACCCTCTGG GTGGCTGGAC AAAGGATGAC 
GATGTTCCTT TGATGTGGCG TATGAAGCAG CATGCTGCAG TGTTGGAGGA AGGAGTTCTG 
AATCCTGAGA CGACAGTGGT GGCCATCTTC CCATCTCCCA TGATGTATGC TGGACCAACT 
GAGGTCCAGT GGCATTGCAG AGCACGGATG GTTGCAGGAG CCAACTTTTA CATTGTTGGA 
CGAGACCCTG CTGGCATGCC TCATCCAGAA ACAGGGAAGG ATCTTTATGA GCCAAGTCAT 
GGTGCCAAAG TGCTGACGAT GGCCCCTGGT TTAATCACTT TGGAAATAGT TCCCTTTCGA 
GTTGCAGCTT ACAACAAGAA AAAGAAGCGT ATGGACTACT ATGACTCTGA ACACCATGAA 
GACTTTGAAT TTATTTCAGG AACACGAATG CGCAAACTTG CTCGAGAAGG CCAGAAACCA 
CCTGAAGGTT TCATGGCTCC CAAGGCTTGG ACCGTGCTGA CAGAATACTA CAAATCCTTG 
GAGAAAGCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPSS1 ( NM_005443.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005434.4 Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mRNA.

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CloneID	OHu13190
Clone ID Related Accession (Same CDS sequence)	NM_005443.4 , NM_005443.5
Accession Version	NM_005443.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-13
Organism	Homo sapiens(human)
Product	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU132232.1, BQ437924.1, BC011392.1 and BU517185.1. On Apr 2, 2004 this sequence version replaced NM_005443.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF105227.1, SRR1803614.127325.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGAGATCC CCGGGAGCCT GTGCAAGAAA GTCAAACTGA GCAATAACGC GCAGAACTGG 
GGAATGCAGA GAGCAACCAA TGTCACCTAC CAAGCCCATC ATGTCAGCAG GAACAAGAGA 
GGTCAGGTGG TGGGGACCAG AGGTGGCTTT CGTGGTTGCA CAGTTTGGCT AACAGGCTTG 
TCTGGAGCGG GAAAGACTAC TGTGAGCATG GCCTTGGAGG AGTACCTGGT TTGTCATGGT 
ATTCCATGCT ACACTCTGGA TGGTGACAAT ATTCGTCAAG GTCTCAATAA AAATCTTGGC 
TTTAGTCCTG AAGACAGAGA AGAGAATGTT CGACGCATCG CAGAAGTTGC TAAACTGTTT 
GCAGATGCTG GCTTAGTGTG CATCACAAGT TTCATATCAC CTTACACTCA GGATCGCAAC 
AATGCAAGGC AAATTCATGA AGGTGCAAGT TTACCGTTTT TTGAAGTATT TGTTGATGCT 
CCTCTGCATG TTTGTGAACA GAGGGATGTC AAAGGACTCT ACAAAAAAGC CCGGGCAGGA 
GAAATTAAAG GTTTCACTGG GATCGATTCT GAATATGAAA AGCCAGAGGC CCCTGAGTTG 
GTGCTGAAAA CAGACTCCTG TGATGTAAAT GACTGTGTCC AGCAAGTTGT GGAACTTCTA 
CAGGAACGGG ATATTGTACC TGTGGATGCA TCTTATGAAG TAAAAGAACT ATATGTGCCA 
GAAAATAAAC TTCATTTGGC AAAAACAGAT GCGGAAACAT TACCAGCACT GAAAATTAAT 
AAAGTGGATA TGCAGTGGGT GCAGGTTTTG GCAGAAGGTT GGGCAACCCC ATTGAATGGC 
TTTATGAGAG AGAGGGAGTA CTTGCAGTGC CTTCATTTTG ATTGTCTTCT GGATGGAGGT 
GTCATTAACT TGTCAGTACC TATAGTTCTG ACTGCGACTC ATGAAGATAA AGAGAGGCTG 
GACGGCTGTA CAGCATTTGC TCTGATGTAT GAGGGCCGCC GTGTGGCCAT TCTTCGCAAT 
CCAGAGTTTT TTGAGCACAG GAAAGAGGAG CGCTGTGCCA GACAGTGGGG AACGACATGC 
AAGAACCACC CCTATATTAA GATGGTGATG GAACAAGGAG ATTGGCTGAT TGGAGGAGAT 
CTTCAAGTCT TGGATCGAGT TTATTGGAAT GATGGTCTTG ATCAGTATCG TCTTACTCCT 
ACTGAGCTAA AGCAGAAATT TAAAGATATG AATGCTGATG CTGTCTTTGC ATTTCAACTA 
CGCAACCCAG TGCACAATGG ACATGCCCTG TTAATGCAGG ATACCCATAA GCAACTTCTA 
GAGAGGGGCT ACCGGCGCCC TGTCCTCCTC CTCCACCCTC TGGGTGGCTG GACAAAGGAT 
GACGATGTTC CTTTGATGTG GCGTATGAAG CAGCATGCTG CAGTGTTGGA GGAAGGAGTT 
CTGAATCCTG AGACGACAGT GGTGGCCATC TTCCCATCTC CCATGATGTA TGCTGGACCA 
ACTGAGGTCC AGTGGCATTG CAGAGCACGG ATGGTTGCAG GAGCCAACTT TTACATTGTT 
GGACGAGACC CTGCTGGCAT GCCTCATCCA GAAACAGGGA AGGATCTTTA TGAGCCAAGT 
CATGGTGCCA AAGTGCTGAC GATGGCCCCT GGTTTAATCA CTTTGGAAAT AGTTCCCTTT 
CGAGTTGCAG CTTACAACAA GAAAAAGAAG CGTATGGACT ACTATGACTC TGAACACCAT 
GAAGACTTTG AATTTATTTC AGGAACACGA ATGCGCAAAC TTGCTCGAGA AGGCCAGAAA 
CCACCTGAAG GTTTCATGGC TCCCAAGGCT TGGACCGTGC TGACAGAATA CTACAAATCC 
TTGGAGAAAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005434.4
CDS	85..1959
Translation	MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

Target ORF information:

RefSeq Version	NM_005443.4
Organism	Homo sapiens(human)
Definition	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005443.4

ORF Insert Sequence:

ATGGAGATCC CCGGGAGCCT GTGCAAGAAA GTCAAACTGA GCAATAACGC GCAGAACTGG 
GGAATGCAGA GAGCAACCAA TGTCACCTAC CAAGCCCATC ATGTCAGCAG GAACAAGAGA 
GGTCAGGTGG TGGGGACCAG AGGTGGCTTT CGTGGTTGCA CAGTTTGGCT AACAGGCTTG 
TCTGGAGCGG GAAAGACTAC TGTGAGCATG GCCTTGGAGG AGTACCTGGT TTGTCATGGT 
ATTCCATGCT ACACTCTGGA TGGTGACAAT ATTCGTCAAG GTCTCAATAA AAATCTTGGC 
TTTAGTCCTG AAGACAGAGA AGAGAATGTT CGACGCATCG CAGAAGTTGC TAAACTGTTT 
GCAGATGCTG GCTTAGTGTG CATCACAAGT TTCATATCAC CTTACACTCA GGATCGCAAC 
AATGCAAGGC AAATTCATGA AGGTGCAAGT TTACCGTTTT TTGAAGTATT TGTTGATGCT 
CCTCTGCATG TTTGTGAACA GAGGGATGTC AAAGGACTCT ACAAAAAAGC CCGGGCAGGA 
GAAATTAAAG GTTTCACTGG GATCGATTCT GAATATGAAA AGCCAGAGGC CCCTGAGTTG 
GTGCTGAAAA CAGACTCCTG TGATGTAAAT GACTGTGTCC AGCAAGTTGT GGAACTTCTA 
CAGGAACGGG ATATTGTACC TGTGGATGCA TCTTATGAAG TAAAAGAACT ATATGTGCCA 
GAAAATAAAC TTCATTTGGC AAAAACAGAT GCGGAAACAT TACCAGCACT GAAAATTAAT 
AAAGTGGATA TGCAGTGGGT GCAGGTTTTG GCAGAAGGTT GGGCAACCCC ATTGAATGGC 
TTTATGAGAG AGAGGGAGTA CTTGCAGTGC CTTCATTTTG ATTGTCTTCT GGATGGAGGT 
GTCATTAACT TGTCAGTACC TATAGTTCTG ACTGCGACTC ATGAAGATAA AGAGAGGCTG 
GACGGCTGTA CAGCATTTGC TCTGATGTAT GAGGGCCGCC GTGTGGCCAT TCTTCGCAAT 
CCAGAGTTTT TTGAGCACAG GAAAGAGGAG CGCTGTGCCA GACAGTGGGG AACGACATGC 
AAGAACCACC CCTATATTAA GATGGTGATG GAACAAGGAG ATTGGCTGAT TGGAGGAGAT 
CTTCAAGTCT TGGATCGAGT TTATTGGAAT GATGGTCTTG ATCAGTATCG TCTTACTCCT 
ACTGAGCTAA AGCAGAAATT TAAAGATATG AATGCTGATG CTGTCTTTGC ATTTCAACTA 
CGCAACCCAG TGCACAATGG ACATGCCCTG TTAATGCAGG ATACCCATAA GCAACTTCTA 
GAGAGGGGCT ACCGGCGCCC TGTCCTCCTC CTCCACCCTC TGGGTGGCTG GACAAAGGAT 
GACGATGTTC CTTTGATGTG GCGTATGAAG CAGCATGCTG CAGTGTTGGA GGAAGGAGTT 
CTGAATCCTG AGACGACAGT GGTGGCCATC TTCCCATCTC CCATGATGTA TGCTGGACCA 
ACTGAGGTCC AGTGGCATTG CAGAGCACGG ATGGTTGCAG GAGCCAACTT TTACATTGTT 
GGACGAGACC CTGCTGGCAT GCCTCATCCA GAAACAGGGA AGGATCTTTA TGAGCCAAGT 
CATGGTGCCA AAGTGCTGAC GATGGCCCCT GGTTTAATCA CTTTGGAAAT AGTTCCCTTT 
CGAGTTGCAG CTTACAACAA GAAAAAGAAG CGTATGGACT ACTATGACTC TGAACACCAT 
GAAGACTTTG AATTTATTTC AGGAACACGA ATGCGCAAAC TTGCTCGAGA AGGCCAGAAA 
CCACCTGAAG GTTTCATGGC TCCCAAGGCT TGGACCGTGC TGACAGAATA CTACAAATCC 
TTGGAGAAAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PAPSS1 ( NM_005443.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005434.4 Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mRNA.

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CloneID	OHu13190
Clone ID Related Accession (Same CDS sequence)	NM_005443.4 , NM_005443.5
Accession Version	NM_005443.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AU132232.1, BQ437924.1 and BC011392.1. On Nov 23, 2018 this sequence version replaced NM_005443.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF105227.1, SRR3476690.94533.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000265174.5/ ENSP00000265174.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGATCC CCGGGAGCCT GTGCAAGAAA GTCAAACTGA GCAATAACGC GCAGAACTGG 
GGAATGCAGA GAGCAACCAA TGTCACCTAC CAAGCCCATC ATGTCAGCAG GAACAAGAGA 
GGTCAGGTGG TGGGGACCAG AGGTGGCTTT CGTGGTTGCA CAGTTTGGCT AACAGGCTTG 
TCTGGAGCGG GAAAGACTAC TGTGAGCATG GCCTTGGAGG AGTACCTGGT TTGTCATGGT 
ATTCCATGCT ACACTCTGGA TGGTGACAAT ATTCGTCAAG GTCTCAATAA AAATCTTGGC 
TTTAGTCCTG AAGACAGAGA AGAGAATGTT CGACGCATCG CAGAAGTTGC TAAACTGTTT 
GCAGATGCTG GCTTAGTGTG CATCACAAGT TTCATATCAC CTTACACTCA GGATCGCAAC 
AATGCAAGGC AAATTCATGA AGGTGCAAGT TTACCGTTTT TTGAAGTATT TGTTGATGCT 
CCTCTGCATG TTTGTGAACA GAGGGATGTC AAAGGACTCT ACAAAAAAGC CCGGGCAGGA 
GAAATTAAAG GTTTCACTGG GATCGATTCT GAATATGAAA AGCCAGAGGC CCCTGAGTTG 
GTGCTGAAAA CAGACTCCTG TGATGTAAAT GACTGTGTCC AGCAAGTTGT GGAACTTCTA 
CAGGAACGGG ATATTGTACC TGTGGATGCA TCTTATGAAG TAAAAGAACT ATATGTGCCA 
GAAAATAAAC TTCATTTGGC AAAAACAGAT GCGGAAACAT TACCAGCACT GAAAATTAAT 
AAAGTGGATA TGCAGTGGGT GCAGGTTTTG GCAGAAGGTT GGGCAACCCC ATTGAATGGC 
TTTATGAGAG AGAGGGAGTA CTTGCAGTGC CTTCATTTTG ATTGTCTTCT GGATGGAGGT 
GTCATTAACT TGTCAGTACC TATAGTTCTG ACTGCGACTC ATGAAGATAA AGAGAGGCTG 
GACGGCTGTA CAGCATTTGC TCTGATGTAT GAGGGCCGCC GTGTGGCCAT TCTTCGCAAT 
CCAGAGTTTT TTGAGCACAG GAAAGAGGAG CGCTGTGCCA GACAGTGGGG AACGACATGC 
AAGAACCACC CCTATATTAA GATGGTGATG GAACAAGGAG ATTGGCTGAT TGGAGGAGAT 
CTTCAAGTCT TGGATCGAGT TTATTGGAAT GATGGTCTTG ATCAGTATCG TCTTACTCCT 
ACTGAGCTAA AGCAGAAATT TAAAGATATG AATGCTGATG CTGTCTTTGC ATTTCAACTA 
CGCAACCCAG TGCACAATGG ACATGCCCTG TTAATGCAGG ATACCCATAA GCAACTTCTA 
GAGAGGGGCT ACCGGCGCCC TGTCCTCCTC CTCCACCCTC TGGGTGGCTG GACAAAGGAT 
GACGATGTTC CTTTGATGTG GCGTATGAAG CAGCATGCTG CAGTGTTGGA GGAAGGAGTT 
CTGAATCCTG AGACGACAGT GGTGGCCATC TTCCCATCTC CCATGATGTA TGCTGGACCA 
ACTGAGGTCC AGTGGCATTG CAGAGCACGG ATGGTTGCAG GAGCCAACTT TTACATTGTT 
GGACGAGACC CTGCTGGCAT GCCTCATCCA GAAACAGGGA AGGATCTTTA TGAGCCAAGT 
CATGGTGCCA AAGTGCTGAC GATGGCCCCT GGTTTAATCA CTTTGGAAAT AGTTCCCTTT 
CGAGTTGCAG CTTACAACAA GAAAAAGAAG CGTATGGACT ACTATGACTC TGAACACCAT 
GAAGACTTTG AATTTATTTC AGGAACACGA ATGCGCAAAC TTGCTCGAGA AGGCCAGAAA 
CCACCTGAAG GTTTCATGGC TCCCAAGGCT TGGACCGTGC TGACAGAATA CTACAAATCC 
TTGGAGAAAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005434.4
CDS	56..1930
Translation	MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGKTTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITSFISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKPEAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKINKVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTAFALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWNDGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLGGWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIVGRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEFISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKA

Target ORF information:

RefSeq Version	NM_005443.5
Organism	Homo sapiens(human)
Definition	Homo sapiens 3'-phosphoadenosine 5'-phosphosulfate synthase 1 (PAPSS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005443.5

ORF Insert Sequence:

ATGGAGATCC CCGGGAGCCT GTGCAAGAAA GTCAAACTGA GCAATAACGC GCAGAACTGG 
GGAATGCAGA GAGCAACCAA TGTCACCTAC CAAGCCCATC ATGTCAGCAG GAACAAGAGA 
GGTCAGGTGG TGGGGACCAG AGGTGGCTTT CGTGGTTGCA CAGTTTGGCT AACAGGCTTG 
TCTGGAGCGG GAAAGACTAC TGTGAGCATG GCCTTGGAGG AGTACCTGGT TTGTCATGGT 
ATTCCATGCT ACACTCTGGA TGGTGACAAT ATTCGTCAAG GTCTCAATAA AAATCTTGGC 
TTTAGTCCTG AAGACAGAGA AGAGAATGTT CGACGCATCG CAGAAGTTGC TAAACTGTTT 
GCAGATGCTG GCTTAGTGTG CATCACAAGT TTCATATCAC CTTACACTCA GGATCGCAAC 
AATGCAAGGC AAATTCATGA AGGTGCAAGT TTACCGTTTT TTGAAGTATT TGTTGATGCT 
CCTCTGCATG TTTGTGAACA GAGGGATGTC AAAGGACTCT ACAAAAAAGC CCGGGCAGGA 
GAAATTAAAG GTTTCACTGG GATCGATTCT GAATATGAAA AGCCAGAGGC CCCTGAGTTG 
GTGCTGAAAA CAGACTCCTG TGATGTAAAT GACTGTGTCC AGCAAGTTGT GGAACTTCTA 
CAGGAACGGG ATATTGTACC TGTGGATGCA TCTTATGAAG TAAAAGAACT ATATGTGCCA 
GAAAATAAAC TTCATTTGGC AAAAACAGAT GCGGAAACAT TACCAGCACT GAAAATTAAT 
AAAGTGGATA TGCAGTGGGT GCAGGTTTTG GCAGAAGGTT GGGCAACCCC ATTGAATGGC 
TTTATGAGAG AGAGGGAGTA CTTGCAGTGC CTTCATTTTG ATTGTCTTCT GGATGGAGGT 
GTCATTAACT TGTCAGTACC TATAGTTCTG ACTGCGACTC ATGAAGATAA AGAGAGGCTG 
GACGGCTGTA CAGCATTTGC TCTGATGTAT GAGGGCCGCC GTGTGGCCAT TCTTCGCAAT 
CCAGAGTTTT TTGAGCACAG GAAAGAGGAG CGCTGTGCCA GACAGTGGGG AACGACATGC 
AAGAACCACC CCTATATTAA GATGGTGATG GAACAAGGAG ATTGGCTGAT TGGAGGAGAT 
CTTCAAGTCT TGGATCGAGT TTATTGGAAT GATGGTCTTG ATCAGTATCG TCTTACTCCT 
ACTGAGCTAA AGCAGAAATT TAAAGATATG AATGCTGATG CTGTCTTTGC ATTTCAACTA 
CGCAACCCAG TGCACAATGG ACATGCCCTG TTAATGCAGG ATACCCATAA GCAACTTCTA 
GAGAGGGGCT ACCGGCGCCC TGTCCTCCTC CTCCACCCTC TGGGTGGCTG GACAAAGGAT 
GACGATGTTC CTTTGATGTG GCGTATGAAG CAGCATGCTG CAGTGTTGGA GGAAGGAGTT 
CTGAATCCTG AGACGACAGT GGTGGCCATC TTCCCATCTC CCATGATGTA TGCTGGACCA 
ACTGAGGTCC AGTGGCATTG CAGAGCACGG ATGGTTGCAG GAGCCAACTT TTACATTGTT 
GGACGAGACC CTGCTGGCAT GCCTCATCCA GAAACAGGGA AGGATCTTTA TGAGCCAAGT 
CATGGTGCCA AAGTGCTGAC GATGGCCCCT GGTTTAATCA CTTTGGAAAT AGTTCCCTTT 
CGAGTTGCAG CTTACAACAA GAAAAAGAAG CGTATGGACT ACTATGACTC TGAACACCAT 
GAAGACTTTG AATTTATTTC AGGAACACGA ATGCGCAAAC TTGCTCGAGA AGGCCAGAAA 
CCACCTGAAG GTTTCATGGC TCCCAAGGCT TGGACCGTGC TGACAGAATA CTACAAATCC 
TTGGAGAAAG CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical cancer research : an official journal of the American Association for Cancer Research23(21)6555-6566(2017 Nov)
Leung AWY,Veinotte CJ,Melong N,Oh MH,Chen K,Enfield KSS,Backstrom I,Warburton C,Yapp D,Berman JN,Bally MB,Lockwood WW

CIB1 contributes to oncogenic signalling by Ras via modulating the subcellular localisation of sphingosine kinase 1.
Oncogene36(18)2619-2627(2017 May)
Zhu W,Gliddon BL,Jarman KE,Moretti PAB,Tin T,Parise LV,Woodcock JM,Powell JA,Ruszkiewicz A,Pitman MR,Pitson SM

Upregulation of UGT2B4 Expression by 3'-Phosphoadenosine-5'-Phosphosulfate Synthase Knockdown: Implications for Coordinated Control of Bile Acid Conjugation.
Drug metabolism and disposition: the biological fate of chemicals43(7)1061-70(2015 Jul)
Barrett KG,Fang H,Cukovic D,Dombkowski AA,Kocarek TA,Runge-Morris M

Leptin induces upregulation of sphingosine kinase 1 in oestrogen receptor-negative breast cancer via Src family kinase-mediated, janus kinase 2-independent pathway.
Breast cancer research : BCR16(5)426(2014 Oct)
Alshaker H,Krell J,Frampton AE,Waxman J,Blyuss O,Zaikin A,Winkler M,Stebbing J,Yag?e E,Pchejetski D

Sphingosine kinase 1 and cancer: a systematic review and meta-analysis.
PloS one9(2)e90362(2014)
Zhang Y,Wang Y,Wan Z,Liu S,Cao Y,Zeng Z

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PAPSS1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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