SPAG9 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPAG9
Entrez Gene ID	9043
Full Name	sperm associated antigen 9
Synonyms	CT89,HLC-6,HLC4,HLC6,JIP-4,JIP4,JLP,PHET,PIG6
General protein information	Preferred Names sperm associated antigen 9 Names C-Jun-amino-terminal kinase-interacting protein 4 JNK interacting protein JNK/SAPK-associated protein Max-binding protein c-Jun NH2-terminal kinase-associated leucine zipper protein cancer/testis antigen 89 human lung cancer oncogene 6 protein lung cancer oncogene 4 mitogen-activated protein kinase 8-interacting protein 4 proliferation-inducing gene 6 protein highly expressed in testis sperm associated antigen 9 variant 5 sperm surface protein sunday driver 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.33
Summary	This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011].
Disorder MIM:	605430

mRNA and Protein(s)

mRNA	Protein	Name
XM_005257771.2	XP_005257828.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
NM_001130527.3	NP_001123999.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform 2
XM_017025284.2	XP_016880773.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
XM_005257774.4	XP_005257831.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
XM_017025285.2	XP_016880774.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
NM_001130528.2	NP_001124000.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform 1
NM_003971.5	NP_003962.3	C-Jun-amino-terminal kinase-interacting protein 4 isoform 3
NM_001130528.3	NP_001124000.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform 1
NM_003971.6	NP_003962.3	C-Jun-amino-terminal kinase-interacting protein 4 isoform 3
XM_005257768.2	XP_005257825.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
XM_017025283.1	XP_016880772.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
XM_017025284.1	XP_016880773.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
XM_017025285.1	XP_016880774.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
NM_001130527.2	NP_001123999.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform 2
XM_005257771.3	XP_005257828.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
NM_001251971.1	NP_001238900.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform 4
XM_005257774.3	XP_005257831.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
NM_001251971.2	NP_001238900.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform 4
XM_017025283.2	XP_016880772.1	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SPAG9	XP_001171158.1
Bos taurus (cattle)	SPAG9	NP_001070393.1
Drosophila melanogaster (fruit fly)	syd	NP_524652.1
Xenopus tropicalis (tropical clawed frog)	spag9	XP_002935990.2
Homo sapiens (human)	SPAG9	NP_003962.3
	SPAG9	XP_001096030.2
Gallus gallus (chicken)	SPAG9	XP_004946363.1
Canis lupus familiaris (dog)	SPAG9	XP_005624735.1
Rattus norvegicus (Norway rat)	Spag9	NP_001101760.2
Mus musculus (house mouse)	Spag9	NP_001186133.1
Danio rerio (zebrafish)	LOC100333761	XP_002663916.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005042	XP_313912.4
Caenorhabditis elegans (roundworm)	unc-16	NP_001122748.1

Gene Ontology
Bibliography

Related articles in PubMed

TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes.
Willett R, Martina JA, Zewe JP, Wills R, Hammond GRV, Puertollano R
Nature communications8(1)1580(2017 Nov)

Aberrant expression of JNK-associated leucine-zipper protein, JLP, promotes accelerated growth of ovarian cancer.
Ha JH, Yan M, Gomathinayagam R, Jayaraman M, Husain S, Liu J, Mukherjee P, Reddy EP, Song YS, Dhanasekaran DN
Oncotarget7(45)72845-72859(2016 Nov)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

SPAG9 promotes endometrial carcinoma cell invasion through regulation of genes related to the epithelial-mesenchymal transition.
Zhang L, Yan L, Cao M, Zhang H, Li C, Bai Y, Yu P, Li M, Zhao X
European journal of gynaecological oncology37(3)312-9(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPAG9 mRNA and protein overexpression in lung cancer tissue, and the presence of SPAG9 IgG antibody in peripheral blood of lung cancer patients indicates that it has potential as a biomarker for lung cancer diagnosis.
Title: Cancer testis antigen SPAG9 is a promising marker for the diagnosis and treatment of lung cancer.

Based on in vitro assays, we found miR-200a-3p significantly inhibit cancer cell proliferation by inducing apoptosis.
Title: MicroRNA-200a-3p suppresses tumor proliferation and induces apoptosis by targeting SPAG9 in renal cell carcinoma.

expression of SPAG9 in ECC cells with TGF-beta1 treatment and spheroids formation was increased
Title: SPAG9 promotes endometrial carcinoma cell invasion through regulation of genes related to the epithelial-mesenchymal transition.

SPAG9-elevated expression contributes to malignant behavior and poor prognosis of breast cancer and may support a potential indicator in treatment selection.
Title: Sperm-associated antigen 9 overexpression correlates with poor prognosis and insensitive to Taxol treatment in breast cancer.

Study suggest that suppression of miR-141 may cause an aberrant overexpression of SPAG9 promoting growth and metastasis of hepatocellular carcinoma cells.
Title: Direct targeting sperm-associated antigen 9 by miR-141 influences hepatocellular carcinoma cell growth and metastasis via JNK pathway.

The following SPAG9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAG9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28444	NM_001130528.3 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu87604	XM_017025283.2 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87605	XM_017025284.2 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87606	XM_017025285.2 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu28428	NM_001251971.2 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu28436	NM_003971.6 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu28461	NM_001130527.3 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu50770	XM_005257771.3 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu50774	XM_005257774.4 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu50767	XM_005257768.2 Latest version!	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu28444	NM_001130528.2	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$459.50-$643.30 $919.00
OHu28436	NM_003971.5	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu87605	XM_017025284.1	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu87606	XM_017025285.1	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu28461	NM_001130527.2	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu50770	XM_005257771.2	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu50774	XM_005257774.3	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu28428	NM_001251971.1	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu87604	XM_017025283.1	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPAG9 ( NM_001130528.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124000.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu28444
Clone ID Related Accession (Same CDS sequence)	NM_001130528.2 , NM_001130528.3
Accession Version	NM_001130528.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091921.1, BC146755.1 and AC005920.1. This sequence is a reference standard in the RefSeqGene project. On Aug 6, 2008 this sequence version replaced NM_001130528.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153878.1, AB011088.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGTCAGCAAT AGTCCTGAAC CTCAGAAGGC TGTAGAACAG 
GAGGATGAGC TTTCTGATGT TAGCCAAGGC GGATCTAAAG CTACCACTCC AGCATCAACA 
GCTAATTCAG ATGTGGCAAC AATTCCTACT GATACTCCCT TAAAGGAAGA AAACGAAGGA 
TTTGTGAAGG TTACAGATGC GCCAAATAAA TCAGAGATAA GCAAACACAT TGAAGTACAG 
GTAGCCCAGG AAACTAGAAA TGTATCTACT GGCTCTGCTG AAAATGAAGA AAAGTCAGAA 
GTTCAAGCAA TCATCGAATC TACTCCTGAG CTGGATATGG ACAAAGATCT CAGTGGATAT 
AAAGGTTCAA GCACTCCCAC CAAAGGCATA GAGAACAAAG CTTTTGATCG CAATACAGAA 
TCTCTCTTTG AAGAACTGTC TTCAGCTGGC TCAGGCCTAA TAGGAGATGT GGATGAAGGA 
GCAGATTTAC TAGGAATGGG TCGGGAAGTT GAGAATCTTA TATTAGAAAA TACACAACTG 
TTGGAAACCA AAAATGCTTT GAACATAGTG AAGAATGATT TGATAGCAAA AGTGGATGAA 
CTGACCTGTG AGAAAGATGT GCTGCAAGGG GAATTGGAGG CTGTGAAGCA AGCCAAACTG 
AAACTAGAGG AAAAGAACAG AGAATTGGAG GAAGAGCTTA GGAAAGCTCG GGCAGAAGCT 
GAAGATGCAA GGCAAAAAGC AAAAGATGAC GATGATAGTG ATATTCCCAC AGCCCAGAGG 
AAACGGTTTA CTAGAGTAGA AATGGCCCGT GTTCTCATGG AGCGAAACCA GTATAAAGAG 
AGATTGATGG AGCTTCAGGA AGCTGTTCGA TGGACAGAGA TGATTCGGGC ATCACGAGAA 
AATCCAGCCA TGCAGGAAAA AAAAAGGTCA AGCATTTGGC AGTTTTTCAG CCGACTTTTC 
AGCTCCTCAA GTAACACGAC TAAGAAGCCT GAACCACCTG TTAATCTGAA GTACAATGCA 
CCCACGTCTC ATGTTACTCC GTCCGTCAAG AAAAGAAGCA GCACCTTATC TCAGCTCCCT 
GGGGATAAGT CCAAAGCCTT TGATTTCCTT AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA 
CGCAGAGAAC AAAAGAGAGA GCAGTATCGT CAGGTAAAAG CACATGTTCA GAAGGAAGAC 
GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG CCTCAGAAGT ACAAACAGGT AACCAATGGT 
CAAGGTGAAA ATAAGATGAA AAATTTACCT GTGCCTGTCT ATCTCAGACC TCTGGATGAA 
AAAGATACAT CAATGAAGCT GTGGTGTGCT GTTGGAGTCA ATTTATCTGG TGGGAAGACC 
AGAGATGGTG GTTCTGTTGT TGGAGCAAGT GTATTTTACA AGGATGTTGC TGGTTTGGAT 
ACAGAAGGCA GTAAACAGCG AAGTGCCTCT CAGAGTAGTT TAGATAAGTT AGATCAGGAA 
CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT CAAGAAGAAT TATCCAGTCT AGTTTGGATC 
TGTACCAGCA CTCATTCGGC TACAAAAGTT CTTATTATTG ATGCTGTTCA ACCTGGCAAC 
ATCCTAGACA GTTTCACTGT TTGCAACTCT CATGTTCTGT GCATTGCAAG TGTGCCAGGT 
GCACGAGAAA CAGACTACCC TGCAGGAGAA GATCTTTCAG AATCTGGTCA GGTAGACAAA 
GCATCTTTAT GTGGAAGTAT GACAAGCAAC AGCTCAGCAG AGACAGACAG CCTGTTAGGA 
GGCATCACAG TGGTTGGTTG TTCTGCAGAA GGTGTGACGG GAGCTGCCAC TTCCCCTAGT 
ACAAATGGTG CTTCTCCAGT GATGGATAAA CCACCAGAAA TGGAAGCAGA AAATAGTGAG 
GTTGATGAAA ATGTTCCAAC AGCAGAAGAA GCAACTGAAG CTACAGAAGG GAATGCGGGG 
TCAGCTGAAG ACACAGTGGA CATCTCCCAA ACTGGCGTCT ACACAGAGCA TGTCTTTACA 
GATCCTTTGG GAGTTCAGAT CCCAGAAGAC CTCTCCCCAG TGTATCAGTC GAGCAATGAC 
TCAGATGCAT ATAAAGATCA AATATCAGTA CTGCCAAATG AACAAGACTT GGTGAGAGAA 
GAAGCCCAGA AAATGAGTAG TCTTTTACCA ACTATGTGGC TTGGAGCTCA AAATGGCTGT 
TTGTATGTCC ATTCATCTGT AGCCCAGTGG AGGAAATGTC TCCATTCCAT TAAACTTAAA 
GATTCGATTC TCAGTATTGT ACACGTGAAG GGAATCGTGT TAGTAGCCCT GGCTGACGGC 
ACCCTTGCAA TCTTTCACAG AGGAGTGGAT GGGCAGTGGG ATTTGTCAAA CTATCACCTC 
TTAGACCTTG GACGGCCTCA TCATTCCATC CGTTGCATGA CTGTGGTACA TGACAAAGTC 
TGGTGTGGCT ATAGGAACAA AATCTATGTG GTGCAGCCAA AGGCCATGAA AATAGAGAAA 
TCTTTTGATG CACATCCCAG GAAGGAGAGC CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT 
GGCGTGTGGG TCTCCATTCG CTTGGATTCT ACGCTCCGTC TCTATCATGC ACACACTTAT 
CAACATCTAC AGGATGTGGA CATTGAGCCT TATGTAAGCA AAATGTTAGG TACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATTACAGCT CTTATGGTGT CTTGTAATCG TTTGTGGGTG 
GGGACAGGAA ATGGTGTCAT TATCTCCATC CCATTGACAG AAACAAATAA AACCTCAGGT 
GTACCAGGAA ATCGTCCTGG AAGTGTAATC CGTGTATATG GTGATGAAAA CAGTGATAAA 
GTGACTCCAG GGACATTTAT ACCCTATTGT TCAATGGCAC ATGCACAGCT TTGCTTCCAT 
GGGCACCGGG ATGCTGTGAA ATTCTTTGTG GCAGTCCCAG GTCAAGTCAT CAGCCCACAA 
AGTAGCAGTA GTGGCACGGA TCTGACGGGT GACAAAGCAG GGCCATCTGC ACAGGAGCCT 
GGTAGTCAGA CGCCCTTGAA GTCTATGCTT GTCATCAGTG GAGGAGAGGG CTACATCGAC 
TTCCGAATGG GTGATGAAGG TGGAGAATCA GAACTTCTTG GAGAGGATCT TCCACTTGAA 
CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC TTGATAGTGT GGCAAGTGAT GTATGGCAAT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124000.1
CDS	210..4175
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_001130528.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130528.2

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGTCAGCAAT AGTCCTGAAC CTCAGAAGGC TGTAGAACAG 
GAGGATGAGC TTTCTGATGT TAGCCAAGGC GGATCTAAAG CTACCACTCC AGCATCAACA 
GCTAATTCAG ATGTGGCAAC AATTCCTACT GATACTCCCT TAAAGGAAGA AAACGAAGGA 
TTTGTGAAGG TTACAGATGC GCCAAATAAA TCAGAGATAA GCAAACACAT TGAAGTACAG 
GTAGCCCAGG AAACTAGAAA TGTATCTACT GGCTCTGCTG AAAATGAAGA AAAGTCAGAA 
GTTCAAGCAA TCATCGAATC TACTCCTGAG CTGGATATGG ACAAAGATCT CAGTGGATAT 
AAAGGTTCAA GCACTCCCAC CAAAGGCATA GAGAACAAAG CTTTTGATCG CAATACAGAA 
TCTCTCTTTG AAGAACTGTC TTCAGCTGGC TCAGGCCTAA TAGGAGATGT GGATGAAGGA 
GCAGATTTAC TAGGAATGGG TCGGGAAGTT GAGAATCTTA TATTAGAAAA TACACAACTG 
TTGGAAACCA AAAATGCTTT GAACATAGTG AAGAATGATT TGATAGCAAA AGTGGATGAA 
CTGACCTGTG AGAAAGATGT GCTGCAAGGG GAATTGGAGG CTGTGAAGCA AGCCAAACTG 
AAACTAGAGG AAAAGAACAG AGAATTGGAG GAAGAGCTTA GGAAAGCTCG GGCAGAAGCT 
GAAGATGCAA GGCAAAAAGC AAAAGATGAC GATGATAGTG ATATTCCCAC AGCCCAGAGG 
AAACGGTTTA CTAGAGTAGA AATGGCCCGT GTTCTCATGG AGCGAAACCA GTATAAAGAG 
AGATTGATGG AGCTTCAGGA AGCTGTTCGA TGGACAGAGA TGATTCGGGC ATCACGAGAA 
AATCCAGCCA TGCAGGAAAA AAAAAGGTCA AGCATTTGGC AGTTTTTCAG CCGACTTTTC 
AGCTCCTCAA GTAACACGAC TAAGAAGCCT GAACCACCTG TTAATCTGAA GTACAATGCA 
CCCACGTCTC ATGTTACTCC GTCCGTCAAG AAAAGAAGCA GCACCTTATC TCAGCTCCCT 
GGGGATAAGT CCAAAGCCTT TGATTTCCTT AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA 
CGCAGAGAAC AAAAGAGAGA GCAGTATCGT CAGGTAAAAG CACATGTTCA GAAGGAAGAC 
GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG CCTCAGAAGT ACAAACAGGT AACCAATGGT 
CAAGGTGAAA ATAAGATGAA AAATTTACCT GTGCCTGTCT ATCTCAGACC TCTGGATGAA 
AAAGATACAT CAATGAAGCT GTGGTGTGCT GTTGGAGTCA ATTTATCTGG TGGGAAGACC 
AGAGATGGTG GTTCTGTTGT TGGAGCAAGT GTATTTTACA AGGATGTTGC TGGTTTGGAT 
ACAGAAGGCA GTAAACAGCG AAGTGCCTCT CAGAGTAGTT TAGATAAGTT AGATCAGGAA 
CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT CAAGAAGAAT TATCCAGTCT AGTTTGGATC 
TGTACCAGCA CTCATTCGGC TACAAAAGTT CTTATTATTG ATGCTGTTCA ACCTGGCAAC 
ATCCTAGACA GTTTCACTGT TTGCAACTCT CATGTTCTGT GCATTGCAAG TGTGCCAGGT 
GCACGAGAAA CAGACTACCC TGCAGGAGAA GATCTTTCAG AATCTGGTCA GGTAGACAAA 
GCATCTTTAT GTGGAAGTAT GACAAGCAAC AGCTCAGCAG AGACAGACAG CCTGTTAGGA 
GGCATCACAG TGGTTGGTTG TTCTGCAGAA GGTGTGACGG GAGCTGCCAC TTCCCCTAGT 
ACAAATGGTG CTTCTCCAGT GATGGATAAA CCACCAGAAA TGGAAGCAGA AAATAGTGAG 
GTTGATGAAA ATGTTCCAAC AGCAGAAGAA GCAACTGAAG CTACAGAAGG GAATGCGGGG 
TCAGCTGAAG ACACAGTGGA CATCTCCCAA ACTGGCGTCT ACACAGAGCA TGTCTTTACA 
GATCCTTTGG GAGTTCAGAT CCCAGAAGAC CTCTCCCCAG TGTATCAGTC GAGCAATGAC 
TCAGATGCAT ATAAAGATCA AATATCAGTA CTGCCAAATG AACAAGACTT GGTGAGAGAA 
GAAGCCCAGA AAATGAGTAG TCTTTTACCA ACTATGTGGC TTGGAGCTCA AAATGGCTGT 
TTGTATGTCC ATTCATCTGT AGCCCAGTGG AGGAAATGTC TCCATTCCAT TAAACTTAAA 
GATTCGATTC TCAGTATTGT ACACGTGAAG GGAATCGTGT TAGTAGCCCT GGCTGACGGC 
ACCCTTGCAA TCTTTCACAG AGGAGTGGAT GGGCAGTGGG ATTTGTCAAA CTATCACCTC 
TTAGACCTTG GACGGCCTCA TCATTCCATC CGTTGCATGA CTGTGGTACA TGACAAAGTC 
TGGTGTGGCT ATAGGAACAA AATCTATGTG GTGCAGCCAA AGGCCATGAA AATAGAGAAA 
TCTTTTGATG CACATCCCAG GAAGGAGAGC CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT 
GGCGTGTGGG TCTCCATTCG CTTGGATTCT ACGCTCCGTC TCTATCATGC ACACACTTAT 
CAACATCTAC AGGATGTGGA CATTGAGCCT TATGTAAGCA AAATGTTAGG TACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATTACAGCT CTTATGGTGT CTTGTAATCG TTTGTGGGTG 
GGGACAGGAA ATGGTGTCAT TATCTCCATC CCATTGACAG AAACAAATAA AACCTCAGGT 
GTACCAGGAA ATCGTCCTGG AAGTGTAATC CGTGTATATG GTGATGAAAA CAGTGATAAA 
GTGACTCCAG GGACATTTAT ACCCTATTGT TCAATGGCAC ATGCACAGCT TTGCTTCCAT 
GGGCACCGGG ATGCTGTGAA ATTCTTTGTG GCAGTCCCAG GTCAAGTCAT CAGCCCACAA 
AGTAGCAGTA GTGGCACGGA TCTGACGGGT GACAAAGCAG GGCCATCTGC ACAGGAGCCT 
GGTAGTCAGA CGCCCTTGAA GTCTATGCTT GTCATCAGTG GAGGAGAGGG CTACATCGAC 
TTCCGAATGG GTGATGAAGG TGGAGAATCA GAACTTCTTG GAGAGGATCT TCCACTTGAA 
CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC TTGATAGTGT GGCAAGTGAT GTATGGCAAT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_001130528.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001124000.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 1, mRNA.

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CloneID	OHu28444
Clone ID Related Accession (Same CDS sequence)	NM_001130528.2 , NM_001130528.3
Accession Version	NM_001130528.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3966bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005839.1, AK091921.1, BC146755.1 and AC005920.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001130528.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC153878.1, AB011088.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262013.12/ ENSP00000262013.7 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGTCAGCAAT AGTCCTGAAC CTCAGAAGGC TGTAGAACAG 
GAGGATGAGC TTTCTGATGT TAGCCAAGGC GGATCTAAAG CTACCACTCC AGCATCAACA 
GCTAATTCAG ATGTGGCAAC AATTCCTACT GATACTCCCT TAAAGGAAGA AAACGAAGGA 
TTTGTGAAGG TTACAGATGC GCCAAATAAA TCAGAGATAA GCAAACACAT TGAAGTACAG 
GTAGCCCAGG AAACTAGAAA TGTATCTACT GGCTCTGCTG AAAATGAAGA AAAGTCAGAA 
GTTCAAGCAA TCATCGAATC TACTCCTGAG CTGGATATGG ACAAAGATCT CAGTGGATAT 
AAAGGTTCAA GCACTCCCAC CAAAGGCATA GAGAACAAAG CTTTTGATCG CAATACAGAA 
TCTCTCTTTG AAGAACTGTC TTCAGCTGGC TCAGGCCTAA TAGGAGATGT GGATGAAGGA 
GCAGATTTAC TAGGAATGGG TCGGGAAGTT GAGAATCTTA TATTAGAAAA TACACAACTG 
TTGGAAACCA AAAATGCTTT GAACATAGTG AAGAATGATT TGATAGCAAA AGTGGATGAA 
CTGACCTGTG AGAAAGATGT GCTGCAAGGG GAATTGGAGG CTGTGAAGCA AGCCAAACTG 
AAACTAGAGG AAAAGAACAG AGAATTGGAG GAAGAGCTTA GGAAAGCTCG GGCAGAAGCT 
GAAGATGCAA GGCAAAAAGC AAAAGATGAC GATGATAGTG ATATTCCCAC AGCCCAGAGG 
AAACGGTTTA CTAGAGTAGA AATGGCCCGT GTTCTCATGG AGCGAAACCA GTATAAAGAG 
AGATTGATGG AGCTTCAGGA AGCTGTTCGA TGGACAGAGA TGATTCGGGC ATCACGAGAA 
AATCCAGCCA TGCAGGAAAA AAAAAGGTCA AGCATTTGGC AGTTTTTCAG CCGACTTTTC 
AGCTCCTCAA GTAACACGAC TAAGAAGCCT GAACCACCTG TTAATCTGAA GTACAATGCA 
CCCACGTCTC ATGTTACTCC GTCCGTCAAG AAAAGAAGCA GCACCTTATC TCAGCTCCCT 
GGGGATAAGT CCAAAGCCTT TGATTTCCTT AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA 
CGCAGAGAAC AAAAGAGAGA GCAGTATCGT CAGGTAAAAG CACATGTTCA GAAGGAAGAC 
GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG CCTCAGAAGT ACAAACAGGT AACCAATGGT 
CAAGGTGAAA ATAAGATGAA AAATTTACCT GTGCCTGTCT ATCTCAGACC TCTGGATGAA 
AAAGATACAT CAATGAAGCT GTGGTGTGCT GTTGGAGTCA ATTTATCTGG TGGGAAGACC 
AGAGATGGTG GTTCTGTTGT TGGAGCAAGT GTATTTTACA AGGATGTTGC TGGTTTGGAT 
ACAGAAGGCA GTAAACAGCG AAGTGCCTCT CAGAGTAGTT TAGATAAGTT AGATCAGGAA 
CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT CAAGAAGAAT TATCCAGTCT AGTTTGGATC 
TGTACCAGCA CTCATTCGGC TACAAAAGTT CTTATTATTG ATGCTGTTCA ACCTGGCAAC 
ATCCTAGACA GTTTCACTGT TTGCAACTCT CATGTTCTGT GCATTGCAAG TGTGCCAGGT 
GCACGAGAAA CAGACTACCC TGCAGGAGAA GATCTTTCAG AATCTGGTCA GGTAGACAAA 
GCATCTTTAT GTGGAAGTAT GACAAGCAAC AGCTCAGCAG AGACAGACAG CCTGTTAGGA 
GGCATCACAG TGGTTGGTTG TTCTGCAGAA GGTGTGACGG GAGCTGCCAC TTCCCCTAGT 
ACAAATGGTG CTTCTCCAGT GATGGATAAA CCACCAGAAA TGGAAGCAGA AAATAGTGAG 
GTTGATGAAA ATGTTCCAAC AGCAGAAGAA GCAACTGAAG CTACAGAAGG GAATGCGGGG 
TCAGCTGAAG ACACAGTGGA CATCTCCCAA ACTGGCGTCT ACACAGAGCA TGTCTTTACA 
GATCCTTTGG GAGTTCAGAT CCCAGAAGAC CTCTCCCCAG TGTATCAGTC GAGCAATGAC 
TCAGATGCAT ATAAAGATCA AATATCAGTA CTGCCAAATG AACAAGACTT GGTGAGAGAA 
GAAGCCCAGA AAATGAGTAG TCTTTTACCA ACTATGTGGC TTGGAGCTCA AAATGGCTGT 
TTGTATGTCC ATTCATCTGT AGCCCAGTGG AGGAAATGTC TCCATTCCAT TAAACTTAAA 
GATTCGATTC TCAGTATTGT ACACGTGAAG GGAATCGTGT TAGTAGCCCT GGCTGACGGC 
ACCCTTGCAA TCTTTCACAG AGGAGTGGAT GGGCAGTGGG ATTTGTCAAA CTATCACCTC 
TTAGACCTTG GACGGCCTCA TCATTCCATC CGTTGCATGA CTGTGGTACA TGACAAAGTC 
TGGTGTGGCT ATAGGAACAA AATCTATGTG GTGCAGCCAA AGGCCATGAA AATAGAGAAA 
TCTTTTGATG CACATCCCAG GAAGGAGAGC CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT 
GGCGTGTGGG TCTCCATTCG CTTGGATTCT ACGCTCCGTC TCTATCATGC ACACACTTAT 
CAACATCTAC AGGATGTGGA CATTGAGCCT TATGTAAGCA AAATGTTAGG TACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATTACAGCT CTTATGGTGT CTTGTAATCG TTTGTGGGTG 
GGGACAGGAA ATGGTGTCAT TATCTCCATC CCATTGACAG AAACAAATAA AACCTCAGGT 
GTACCAGGAA ATCGTCCTGG AAGTGTAATC CGTGTATATG GTGATGAAAA CAGTGATAAA 
GTGACTCCAG GGACATTTAT ACCCTATTGT TCAATGGCAC ATGCACAGCT TTGCTTCCAT 
GGGCACCGGG ATGCTGTGAA ATTCTTTGTG GCAGTCCCAG GTCAAGTCAT CAGCCCACAA 
AGTAGCAGTA GTGGCACGGA TCTGACGGGT GACAAAGCAG GGCCATCTGC ACAGGAGCCT 
GGTAGTCAGA CGCCCTTGAA GTCTATGCTT GTCATCAGTG GAGGAGAGGG CTACATCGAC 
TTCCGAATGG GTGATGAAGG TGGAGAATCA GAACTTCTTG GAGAGGATCT TCCACTTGAA 
CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC TTGATAGTGT GGCAAGTGAT GTATGGCAAT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001124000.1
CDS	213..4178
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_001130528.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130528.3

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGTCAGCAAT AGTCCTGAAC CTCAGAAGGC TGTAGAACAG 
GAGGATGAGC TTTCTGATGT TAGCCAAGGC GGATCTAAAG CTACCACTCC AGCATCAACA 
GCTAATTCAG ATGTGGCAAC AATTCCTACT GATACTCCCT TAAAGGAAGA AAACGAAGGA 
TTTGTGAAGG TTACAGATGC GCCAAATAAA TCAGAGATAA GCAAACACAT TGAAGTACAG 
GTAGCCCAGG AAACTAGAAA TGTATCTACT GGCTCTGCTG AAAATGAAGA AAAGTCAGAA 
GTTCAAGCAA TCATCGAATC TACTCCTGAG CTGGATATGG ACAAAGATCT CAGTGGATAT 
AAAGGTTCAA GCACTCCCAC CAAAGGCATA GAGAACAAAG CTTTTGATCG CAATACAGAA 
TCTCTCTTTG AAGAACTGTC TTCAGCTGGC TCAGGCCTAA TAGGAGATGT GGATGAAGGA 
GCAGATTTAC TAGGAATGGG TCGGGAAGTT GAGAATCTTA TATTAGAAAA TACACAACTG 
TTGGAAACCA AAAATGCTTT GAACATAGTG AAGAATGATT TGATAGCAAA AGTGGATGAA 
CTGACCTGTG AGAAAGATGT GCTGCAAGGG GAATTGGAGG CTGTGAAGCA AGCCAAACTG 
AAACTAGAGG AAAAGAACAG AGAATTGGAG GAAGAGCTTA GGAAAGCTCG GGCAGAAGCT 
GAAGATGCAA GGCAAAAAGC AAAAGATGAC GATGATAGTG ATATTCCCAC AGCCCAGAGG 
AAACGGTTTA CTAGAGTAGA AATGGCCCGT GTTCTCATGG AGCGAAACCA GTATAAAGAG 
AGATTGATGG AGCTTCAGGA AGCTGTTCGA TGGACAGAGA TGATTCGGGC ATCACGAGAA 
AATCCAGCCA TGCAGGAAAA AAAAAGGTCA AGCATTTGGC AGTTTTTCAG CCGACTTTTC 
AGCTCCTCAA GTAACACGAC TAAGAAGCCT GAACCACCTG TTAATCTGAA GTACAATGCA 
CCCACGTCTC ATGTTACTCC GTCCGTCAAG AAAAGAAGCA GCACCTTATC TCAGCTCCCT 
GGGGATAAGT CCAAAGCCTT TGATTTCCTT AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA 
CGCAGAGAAC AAAAGAGAGA GCAGTATCGT CAGGTAAAAG CACATGTTCA GAAGGAAGAC 
GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG CCTCAGAAGT ACAAACAGGT AACCAATGGT 
CAAGGTGAAA ATAAGATGAA AAATTTACCT GTGCCTGTCT ATCTCAGACC TCTGGATGAA 
AAAGATACAT CAATGAAGCT GTGGTGTGCT GTTGGAGTCA ATTTATCTGG TGGGAAGACC 
AGAGATGGTG GTTCTGTTGT TGGAGCAAGT GTATTTTACA AGGATGTTGC TGGTTTGGAT 
ACAGAAGGCA GTAAACAGCG AAGTGCCTCT CAGAGTAGTT TAGATAAGTT AGATCAGGAA 
CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT CAAGAAGAAT TATCCAGTCT AGTTTGGATC 
TGTACCAGCA CTCATTCGGC TACAAAAGTT CTTATTATTG ATGCTGTTCA ACCTGGCAAC 
ATCCTAGACA GTTTCACTGT TTGCAACTCT CATGTTCTGT GCATTGCAAG TGTGCCAGGT 
GCACGAGAAA CAGACTACCC TGCAGGAGAA GATCTTTCAG AATCTGGTCA GGTAGACAAA 
GCATCTTTAT GTGGAAGTAT GACAAGCAAC AGCTCAGCAG AGACAGACAG CCTGTTAGGA 
GGCATCACAG TGGTTGGTTG TTCTGCAGAA GGTGTGACGG GAGCTGCCAC TTCCCCTAGT 
ACAAATGGTG CTTCTCCAGT GATGGATAAA CCACCAGAAA TGGAAGCAGA AAATAGTGAG 
GTTGATGAAA ATGTTCCAAC AGCAGAAGAA GCAACTGAAG CTACAGAAGG GAATGCGGGG 
TCAGCTGAAG ACACAGTGGA CATCTCCCAA ACTGGCGTCT ACACAGAGCA TGTCTTTACA 
GATCCTTTGG GAGTTCAGAT CCCAGAAGAC CTCTCCCCAG TGTATCAGTC GAGCAATGAC 
TCAGATGCAT ATAAAGATCA AATATCAGTA CTGCCAAATG AACAAGACTT GGTGAGAGAA 
GAAGCCCAGA AAATGAGTAG TCTTTTACCA ACTATGTGGC TTGGAGCTCA AAATGGCTGT 
TTGTATGTCC ATTCATCTGT AGCCCAGTGG AGGAAATGTC TCCATTCCAT TAAACTTAAA 
GATTCGATTC TCAGTATTGT ACACGTGAAG GGAATCGTGT TAGTAGCCCT GGCTGACGGC 
ACCCTTGCAA TCTTTCACAG AGGAGTGGAT GGGCAGTGGG ATTTGTCAAA CTATCACCTC 
TTAGACCTTG GACGGCCTCA TCATTCCATC CGTTGCATGA CTGTGGTACA TGACAAAGTC 
TGGTGTGGCT ATAGGAACAA AATCTATGTG GTGCAGCCAA AGGCCATGAA AATAGAGAAA 
TCTTTTGATG CACATCCCAG GAAGGAGAGC CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT 
GGCGTGTGGG TCTCCATTCG CTTGGATTCT ACGCTCCGTC TCTATCATGC ACACACTTAT 
CAACATCTAC AGGATGTGGA CATTGAGCCT TATGTAAGCA AAATGTTAGG TACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATTACAGCT CTTATGGTGT CTTGTAATCG TTTGTGGGTG 
GGGACAGGAA ATGGTGTCAT TATCTCCATC CCATTGACAG AAACAAATAA AACCTCAGGT 
GTACCAGGAA ATCGTCCTGG AAGTGTAATC CGTGTATATG GTGATGAAAA CAGTGATAAA 
GTGACTCCAG GGACATTTAT ACCCTATTGT TCAATGGCAC ATGCACAGCT TTGCTTCCAT 
GGGCACCGGG ATGCTGTGAA ATTCTTTGTG GCAGTCCCAG GTCAAGTCAT CAGCCCACAA 
AGTAGCAGTA GTGGCACGGA TCTGACGGGT GACAAAGCAG GGCCATCTGC ACAGGAGCCT 
GGTAGTCAGA CGCCCTTGAA GTCTATGCTT GTCATCAGTG GAGGAGAGGG CTACATCGAC 
TTCCGAATGG GTGATGAAGG TGGAGAATCA GAACTTCTTG GAGAGGATCT TCCACTTGAA 
CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC TTGATAGTGT GGCAAGTGAT GTATGGCAAT 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_017025283.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880772.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu87604
Clone ID Related Accession (Same CDS sequence)	XM_017025283.1 , XM_017025283.2
Accession Version	XM_017025283.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACCGTAA TCCTCCACCA GGGACGTTTA 
CTGGGGCTGA GGGCAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880772.1
CDS	225..3800
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_017025283.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025283.1

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACCGTAA TCCTCCACCA GGGACGTTTA 
CTGGGGCTGA GGGCAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_017025283.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880772.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.

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CloneID	OHu87604
Clone ID Related Accession (Same CDS sequence)	XM_017025283.1 , XM_017025283.2
Accession Version	XM_017025283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACCGTAA TCCTCCACCA GGGACGTTTA 
CTGGGGCTGA GGGCAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880772.1
CDS	219..3794
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_017025283.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025283.2

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACCGTAA TCCTCCACCA GGGACGTTTA 
CTGGGGCTGA GGGCAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_017025284.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880773.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu87605
Clone ID Related Accession (Same CDS sequence)	XM_017025284.2 , XM_017025284.1
Accession Version	XM_017025284.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025284.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACAAATA AAACCTCAGG TGTACCAGGA 
AATCGTCCTG GAAGTGTAAT CCGTGTATAT GGTGATGAAA ACAGTGATAA AGTGACTCCA 
GGGACATTTA TACCCTATTG TTCAATGGCA CATGCACAGC TTTGCTTCCA TGGGCACCGG 
GATGCTGTGA AATTCTTTGT GGCAGTCCCA GGTCAAGTCA TCAGCCCACA AAGTAGCAGT 
AGTGGCACGG ATCTGACGGG TGACAAAGCA GGGCCATCTG CACAGGAGCC TGGTAGTCAG 
ACGCCCTTGA AGTCTATGCT TGTCATCAGT GGAGGAGAGG GCTACATCGA CTTCCGAATG 
GGTGATGAAG GTGGAGAATC AGAACTTCTT GGAGAGGATC TTCCACTTGA ACCTTCTGTC 
ACCAAAGCAG AAAGGAGTCA CTTGATAGTG TGGCAAGTGA TGTATGGCAA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880773.1
CDS	219..3755
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_017025284.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025284.2

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACAAATA AAACCTCAGG TGTACCAGGA 
AATCGTCCTG GAAGTGTAAT CCGTGTATAT GGTGATGAAA ACAGTGATAA AGTGACTCCA 
GGGACATTTA TACCCTATTG TTCAATGGCA CATGCACAGC TTTGCTTCCA TGGGCACCGG 
GATGCTGTGA AATTCTTTGT GGCAGTCCCA GGTCAAGTCA TCAGCCCACA AAGTAGCAGT 
AGTGGCACGG ATCTGACGGG TGACAAAGCA GGGCCATCTG CACAGGAGCC TGGTAGTCAG 
ACGCCCTTGA AGTCTATGCT TGTCATCAGT GGAGGAGAGG GCTACATCGA CTTCCGAATG 
GGTGATGAAG GTGGAGAATC AGAACTTCTT GGAGAGGATC TTCCACTTGA ACCTTCTGTC 
ACCAAAGCAG AAAGGAGTCA CTTGATAGTG TGGCAAGTGA TGTATGGCAA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_017025284.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880773.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu87605
Clone ID Related Accession (Same CDS sequence)	XM_017025284.2 , XM_017025284.1
Accession Version	XM_017025284.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACAAATA AAACCTCAGG TGTACCAGGA 
AATCGTCCTG GAAGTGTAAT CCGTGTATAT GGTGATGAAA ACAGTGATAA AGTGACTCCA 
GGGACATTTA TACCCTATTG TTCAATGGCA CATGCACAGC TTTGCTTCCA TGGGCACCGG 
GATGCTGTGA AATTCTTTGT GGCAGTCCCA GGTCAAGTCA TCAGCCCACA AAGTAGCAGT 
AGTGGCACGG ATCTGACGGG TGACAAAGCA GGGCCATCTG CACAGGAGCC TGGTAGTCAG 
ACGCCCTTGA AGTCTATGCT TGTCATCAGT GGAGGAGAGG GCTACATCGA CTTCCGAATG 
GGTGATGAAG GTGGAGAATC AGAACTTCTT GGAGAGGATC TTCCACTTGA ACCTTCTGTC 
ACCAAAGCAG AAAGGAGTCA CTTGATAGTG TGGCAAGTGA TGTATGGCAA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880773.1
CDS	225..3761
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_017025284.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025284.1

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGTCAGCAA TAGTCCTGAA CCTCAGAAGG CTGTAGAACA GGAGGATGAG 
CTTTCTGATG TTAGCCAAGG CGGATCTAAA GCTACCACTC CAGCATCAAC AGCTAATTCA 
GATGTGGCAA CAATTCCTAC TGATACTCCC TTAAAGGAAG AAAACGAAGG ATTTGTGAAG 
GTTACAGATG CGCCAAATAA ATCAGAGATA AGCAAACACA TTGAAGTACA GGTAGCCCAG 
GAAACTAGAA ATGTATCTAC TGGCTCTGCT GAAAATGAAG AAAAGTCAGA AGTTCAAGCA 
ATCATCGAAT CTACTCCTGA GCTGGATATG GACAAAGATC TCAGTGGATA TAAAGGTTCA 
AGCACTCCCA CCAAAGGCAT AGAGAACAAA GCTTTTGATC GCAATACAGA ATCTCTCTTT 
GAAGAACTGT CTTCAGCTGG CTCAGGCCTA ATAGGAGATG TGGATGAAGG AGCAGATTTA 
CTAGGAATGG GTCGGGAAGT TGAGAATCTT ATATTAGAAA ATACACAACT GTTGGAAACC 
AAAAATGCTT TGAACATAGT GAAGAATGAT TTGATAGCAA AAGTGGATGA ACTGACCTGT 
GAGAAAGATG TGCTGCAAGG GGAATTGGAG GCTGTGAAGC AAGCCAAACT GAAACTAGAG 
GAAAAGAACA GAGAATTGGA GGAAGAGCTT AGGAAAGCTC GGGCAGAAGC TGAAGATGCA 
AGGCAAAAAG CAAAAGATGA CGATGATAGT GATATTCCCA CAGCCCAGAG GAAACGGTTT 
ACTAGAGTAG AAATGGCCCG TGTTCTCATG GAGCGAAACC AGTATAAAGA GAGATTGATG 
GAGCTTCAGG AAGCTGTTCG ATGGACAGAG ATGATTCGGG CATCACGAGA AAATCCAGCC 
ATGCAGGAAA AAAAAAGGTC AAGCATTTGG CAGTTTTTCA GCCGACTTTT CAGCTCCTCA 
AGTAACACGA CTAAGAAGCC TGAACCACCT GTTAATCTGA AGTACAATGC ACCCACGTCT 
CATGTTACTC CGTCCGTCAA GAAAAGAAGC AGCACCTTAT CTCAGCTCCC TGGGGATAAG 
TCCAAAGCCT TTGATTTCCT TAGTGAAGAA ACTGAAGCTA GTTTAGCCTC ACGCAGAGAA 
CAAAAGAGAG AGCAGTATCG TCAGGTAAAA GCACATGTTC AGAAGGAAGA CGGTAGAGTG 
CAGGCTTTTG GCTGGAGTCT GCCTCAGAAG TACAAACAGG TAACCAATGG TCAAGGTGAA 
AATAAGATGA AAAATTTACC TGTGCCTGTC TATCTCAGAC CTCTGGATGA AAAAGATACA 
TCAATGAAGC TGTGGTGTGC TGTTGGAGTC AATTTATCTG GTGGGAAGAC CAGAGATGGT 
GGTTCTGTTG TTGGAGCAAG TGTATTTTAC AAGGATGTTG CTGGTTTGGA TACAGAAGGC 
AGTAAACAGC GAAGTGCCTC TCAGAGTAGT TTAGATAAGT TAGATCAGGA ACTTAAGGAA 
CAGCAGAAGG AGTTAAAAAA TCAAGAAGAA TTATCCAGTC TAGTTTGGAT CTGTACCAGC 
ACTCATTCGG CTACAAAAGT TCTTATTATT GATGCTGTTC AACCTGGCAA CATCCTAGAC 
AGTTTCACTG TTTGCAACTC TCATGTTCTG TGCATTGCAA GTGTGCCAGG TGCACGAGAA 
ACAGACTACC CTGCAGGAGA AGATCTTTCA GAATCTGGTC AGGTAGACAA AGCATCTTTA 
TGTGGAAGTA TGACAAGCAA CAGCTCAGCA GAGACAGACA GCCTGTTAGG AGGCATCACA 
GTGGTTGGTT GTTCTGCAGA AGGTGTGACG GGAGCTGCCA CTTCCCCTAG TACAAATGGT 
GCTTCTCCAG TGATGGATAA ACCACCAGAA ATGGAAGCAG AAAATAGTGA GGTTGATGAA 
AATGTTCCAA CAGCAGAAGA AGCAACTGAA GCTACAGAAG GGAATGCGGG GTCAGCTGAA 
GACACAGTGG ACATCTCCCA AACTGGCGTC TACACAGAGC ATGTCTTTAC AGATCCTTTG 
GGAGTTCAGA TCCCAGAAGA CCTCTCCCCA GTGTATCAGT CGAGCAATGA CTCAGATGCA 
TATAAAGATC AAATATCAGT ACTGCCAAAT GAACAAGACT TGGTGAGAGA AGAAGCCCAG 
AAAATGAGTA GTCTTTTACC AACTATGTGG CTTGGAGCTC AAAATGGCTG TTTGTATGTC 
CATTCATCTG TAGCCCAGTG GAGGAAATGT CTCCATTCCA TTAAACTTAA AGATTCGATT 
CTCAGTATTG TACACGTGAA GGGAATCGTG TTAGTAGCCC TGGCTGACGG CACCCTTGCA 
ATCTTTCACA GAGGAGTGGA TGGGCAGTGG GATTTGTCAA ACTATCACCT CTTAGACCTT 
GGACGGCCTC ATCATTCCAT CCGTTGCATG ACTGTGGTAC ATGACAAAGT CTGGTGTGGC 
TATAGGAACA AAATCTATGT GGTGCAGCCA AAGGCCATGA AAATAGAGAA ATCTTTTGAT 
GCACATCCCA GGAAGGAGAG CCAAGTGCGA CAGCTTGCGT GGGTGGGGGA TGGCGTGTGG 
GTCTCCATTC GCTTGGATTC TACGCTCCGT CTCTATCATG CACACACTTA TCAACATCTA 
CAGGATGTGG ACATTGAGCC TTATGTAAGC AAAATGTTAG GTACTGGAAA ACTGGGCTTC 
TCTTTTGTGA GAATTACAGC TCTTATGGTG TCTTGTAATC GTTTGTGGGT GGGGACAGGA 
AATGGTGTCA TTATCTCCAT CCCATTGACA GAAACAAATA AAACCTCAGG TGTACCAGGA 
AATCGTCCTG GAAGTGTAAT CCGTGTATAT GGTGATGAAA ACAGTGATAA AGTGACTCCA 
GGGACATTTA TACCCTATTG TTCAATGGCA CATGCACAGC TTTGCTTCCA TGGGCACCGG 
GATGCTGTGA AATTCTTTGT GGCAGTCCCA GGTCAAGTCA TCAGCCCACA AAGTAGCAGT 
AGTGGCACGG ATCTGACGGG TGACAAAGCA GGGCCATCTG CACAGGAGCC TGGTAGTCAG 
ACGCCCTTGA AGTCTATGCT TGTCATCAGT GGAGGAGAGG GCTACATCGA CTTCCGAATG 
GGTGATGAAG GTGGAGAATC AGAACTTCTT GGAGAGGATC TTCCACTTGA ACCTTCTGTC 
ACCAAAGCAG AAAGGAGTCA CTTGATAGTG TGGCAAGTGA TGTATGGCAA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_017025285.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880774.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.

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CloneID	OHu87606
Clone ID Related Accession (Same CDS sequence)	XM_017025285.2 , XM_017025285.1
Accession Version	XM_017025285.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017025285.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCATA ATTATATGGA ACATTTAGAA AGAACAAAAC TTCATCAGCT CTCAGGGAGT 
GATCAACTAG AATCCACAGC TCATAGTAGA ATTAGAAAAG AACGCCCTAT ATCATTAGGA 
ATTTTCCCAT TACCTGCTGG AGATGGATTG CTTACACCTG ATGCTCAGAA AGGAGGAGAG 
ACCCCTGGAT CTGAGCAATG GAAATTTCAG GAATTAAGTC AACCACGTTC TCATACCAGC 
CTGAAGGTCA GCAATAGTCC TGAACCTCAG AAGGCTGTAG AACAGGAGGA TGAGCTTTCT 
GATGTTAGCC AAGGCGGATC TAAAGCTACC ACTCCAGCAT CAACAGCTAA TTCAGATGTG 
GCAACAATTC CTACTGATAC TCCCTTAAAG GAAGAAAACG AAGGATTTGT GAAGGTTACA 
GATGCGCCAA ATAAATCAGA GATAAGCAAA CACATTGAAG TACAGGTAGC CCAGGAAACT 
AGAAATGTAT CTACTGGCTC TGCTGAAAAT GAAGAAAAGT CAGAAGTTCA AGCAATCATC 
GAATCTACTC CTGAGCTGGA TATGGACAAA GATCTCAGTG GATATAAAGG TTCAAGCACT 
CCCACCAAAG GCATAGAGAA CAAAGCTTTT GATCGCAATA CAGAATCTCT CTTTGAAGAA 
CTGTCTTCAG CTGGCTCAGG CCTAATAGGA GATGTGGATG AAGGAGCAGA TTTACTAGGA 
ATGGGTCGGG AAGTTGAGAA TCTTATATTA GAAAATACAC AACTGTTGGA AACCAAAAAT 
GCTTTGAACA TAGTGAAGAA TGATTTGATA GCAAAAGTGG ATGAACTGAC CTGTGAGAAA 
GATGTGCTGC AAGGGGAATT GGAGGCTGTG AAGCAAGCCA AACTGAAACT AGAGGAAAAG 
AACAGAGAAT TGGAGGAAGA GCTTAGGAAA GCTCGGGCAG AAGCTGAAGA TGCAAGGCAA 
AAAGCAAAAG ATGACGATGA TAGTGATATT CCCACAGCCC AGAGGAAACG GTTTACTAGA 
GTAGAAATGG CCCGTGTTCT CATGGAGCGA AACCAGTATA AAGAGAGATT GATGGAGCTT 
CAGGAAGCTG TTCGATGGAC AGAGATGATT CGGGCATCAC GAGAAAATCC AGCCATGCAG 
GAAAAAAAAA GGTCAAGCAT TTGGCAGTTT TTCAGCCGAC TTTTCAGCTC CTCAAGTAAC 
ACGACTAAGA AGCCTGAACC ACCTGTTAAT CTGAAGTACA ATGCACCCAC GTCTCATGTT 
ACTCCGTCCG TCAAGAAAAG AAGCAGCACC TTATCTCAGC TCCCTGGGGA TAAGTCCAAA 
GCCTTTGATT TCCTTAGTGA AGAAACTGAA GCTAGTTTAG CCTCACGCAG AGAACAAAAG 
AGAGAGCAGT ATCGTCAGGT AAAAGCACAT GTTCAGAAGG AAGACGGTAG AGTGCAGGCT 
TTTGGCTGGA GTCTGCCTCA GAAGTACAAA CAGGTAACCA ATGGTCAAGG TGAAAATAAG 
ATGAAAAATT TACCTGTGCC TGTCTATCTC AGACCTCTGG ATGAAAAAGA TACATCAATG 
AAGCTGTGGT GTGCTGTTGG AGTCAATTTA TCTGGTGGGA AGACCAGAGA TGGTGGTTCT 
GTTGTTGGAG CAAGTGTATT TTACAAGGAT GTTGCTGGTT TGGATACAGA AGGCAGTAAA 
CAGCGAAGTG CCTCTCAGAG TAGTTTAGAT AAGTTAGATC AGGAACTTAA GGAACAGCAG 
AAGGAGTTAA AAAATCAAGA AGAATTATCC AGTCTAGTTT GGATCTGTAC CAGCACTCAT 
TCGGCTACAA AAGTTCTTAT TATTGATGCT GTTCAACCTG GCAACATCCT AGACAGTTTC 
ACTGTTTGCA ACTCTCATGT TCTGTGCATT GCAAGTGTGC CAGGTGCACG AGAAACAGAC 
TACCCTGCAG GAGAAGATCT TTCAGAATCT GGTCAGGTAG ACAAAGCATC TTTATGTGGA 
AGTATGACAA GCAACAGCTC AGCAGAGACA GACAGCCTGT TAGGAGGCAT CACAGTGGTT 
GGTTGTTCTG CAGAAGGTGT GACGGGAGCT GCCACTTCCC CTAGTACAAA TGGTGCTTCT 
CCAGTGATGG ATAAACCACC AGAAATGGAA GCAGAAAATA GTGAGGTTGA TGAAAATGTT 
CCAACAGCAG AAGAAGCAAC TGAAGCTACA GAAGGGAATG CGGGGTCAGC TGAAGACACA 
GTGGACATCT CCCAAACTGG CGTCTACACA GAGCATGTCT TTACAGATCC TTTGGGAGTT 
CAGATCCCAG AAGACCTCTC CCCAGTGTAT CAGTCGAGCA ATGACTCAGA TGCATATAAA 
GATCAAATAT CAGTACTGCC AAATGAACAA GACTTGGTGA GAGAAGAAGC CCAGAAAATG 
AGTAGTCTTT TACCAACTAT GTGGCTTGGA GCTCAAAATG GCTGTTTGTA TGTCCATTCA 
TCTGTAGCCC AGTGGAGGAA ATGTCTCCAT TCCATTAAAC TTAAAGATTC GATTCTCAGT 
ATTGTACACG TGAAGGGAAT CGTGTTAGTA GCCCTGGCTG ACGGCACCCT TGCAATCTTT 
CACAGAGGAG TGGATGGGCA GTGGGATTTG TCAAACTATC ACCTCTTAGA CCTTGGACGG 
CCTCATCATT CCATCCGTTG CATGACTGTG GTACATGACA AAGTCTGGTG TGGCTATAGG 
AACAAAATCT ATGTGGTGCA GCCAAAGGCC ATGAAAATAG AGAAATCTTT TGATGCACAT 
CCCAGGAAGG AGAGCCAAGT GCGACAGCTT GCGTGGGTGG GGGATGGCGT GTGGGTCTCC 
ATTCGCTTGG ATTCTACGCT CCGTCTCTAT CATGCACACA CTTATCAACA TCTACAGGAT 
GTGGACATTG AGCCTTATGT AAGCAAAATG TTAGGTACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATTA CAGCTCTTAT GGTGTCTTGT AATCGTTTGT GGGTGGGGAC AGGAAATGGT 
GTCATTATCT CCATCCCATT GACAGAAACC GTAATCCTCC ACCAGGGACG TTTACTGGGG 
CTGAGGGCAA ATAAAACCTC AGGTGTACCA GGAAATCGTC CTGGAAGTGT AATCCGTGTA 
TATGGTGATG AAAACAGTGA TAAAGTGACT CCAGGGACAT TTATACCCTA TTGTTCAATG 
GCACATGCAC AGCTTTGCTT CCATGGGCAC CGGGATGCTG TGAAATTCTT TGTGGCAGTC 
CCAGGTCAAG TCATCAGCCC ACAAAGTAGC AGTAGTGGCA CGGATCTGAC GGGTGACAAA 
GCAGGGCCAT CTGCACAGGA GCCTGGTAGT CAGACGCCCT TGAAGTCTAT GCTTGTCATC 
AGTGGAGGAG AGGGCTACAT CGACTTCCGA ATGGGTGATG AAGGTGGAGA ATCAGAACTT 
CTTGGAGAGG ATCTTCCACT TGAACCTTCT GTCACCAAAG CAGAAAGGAG TCACTTGATA 
GTGTGGCAAG TGATGTATGG CAATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880774.1
CDS	136..3645
Translation	MIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_017025285.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025285.2

ORF Insert Sequence:

ATGATCCATA ATTATATGGA ACATTTAGAA AGAACAAAAC TTCATCAGCT CTCAGGGAGT 
GATCAACTAG AATCCACAGC TCATAGTAGA ATTAGAAAAG AACGCCCTAT ATCATTAGGA 
ATTTTCCCAT TACCTGCTGG AGATGGATTG CTTACACCTG ATGCTCAGAA AGGAGGAGAG 
ACCCCTGGAT CTGAGCAATG GAAATTTCAG GAATTAAGTC AACCACGTTC TCATACCAGC 
CTGAAGGTCA GCAATAGTCC TGAACCTCAG AAGGCTGTAG AACAGGAGGA TGAGCTTTCT 
GATGTTAGCC AAGGCGGATC TAAAGCTACC ACTCCAGCAT CAACAGCTAA TTCAGATGTG 
GCAACAATTC CTACTGATAC TCCCTTAAAG GAAGAAAACG AAGGATTTGT GAAGGTTACA 
GATGCGCCAA ATAAATCAGA GATAAGCAAA CACATTGAAG TACAGGTAGC CCAGGAAACT 
AGAAATGTAT CTACTGGCTC TGCTGAAAAT GAAGAAAAGT CAGAAGTTCA AGCAATCATC 
GAATCTACTC CTGAGCTGGA TATGGACAAA GATCTCAGTG GATATAAAGG TTCAAGCACT 
CCCACCAAAG GCATAGAGAA CAAAGCTTTT GATCGCAATA CAGAATCTCT CTTTGAAGAA 
CTGTCTTCAG CTGGCTCAGG CCTAATAGGA GATGTGGATG AAGGAGCAGA TTTACTAGGA 
ATGGGTCGGG AAGTTGAGAA TCTTATATTA GAAAATACAC AACTGTTGGA AACCAAAAAT 
GCTTTGAACA TAGTGAAGAA TGATTTGATA GCAAAAGTGG ATGAACTGAC CTGTGAGAAA 
GATGTGCTGC AAGGGGAATT GGAGGCTGTG AAGCAAGCCA AACTGAAACT AGAGGAAAAG 
AACAGAGAAT TGGAGGAAGA GCTTAGGAAA GCTCGGGCAG AAGCTGAAGA TGCAAGGCAA 
AAAGCAAAAG ATGACGATGA TAGTGATATT CCCACAGCCC AGAGGAAACG GTTTACTAGA 
GTAGAAATGG CCCGTGTTCT CATGGAGCGA AACCAGTATA AAGAGAGATT GATGGAGCTT 
CAGGAAGCTG TTCGATGGAC AGAGATGATT CGGGCATCAC GAGAAAATCC AGCCATGCAG 
GAAAAAAAAA GGTCAAGCAT TTGGCAGTTT TTCAGCCGAC TTTTCAGCTC CTCAAGTAAC 
ACGACTAAGA AGCCTGAACC ACCTGTTAAT CTGAAGTACA ATGCACCCAC GTCTCATGTT 
ACTCCGTCCG TCAAGAAAAG AAGCAGCACC TTATCTCAGC TCCCTGGGGA TAAGTCCAAA 
GCCTTTGATT TCCTTAGTGA AGAAACTGAA GCTAGTTTAG CCTCACGCAG AGAACAAAAG 
AGAGAGCAGT ATCGTCAGGT AAAAGCACAT GTTCAGAAGG AAGACGGTAG AGTGCAGGCT 
TTTGGCTGGA GTCTGCCTCA GAAGTACAAA CAGGTAACCA ATGGTCAAGG TGAAAATAAG 
ATGAAAAATT TACCTGTGCC TGTCTATCTC AGACCTCTGG ATGAAAAAGA TACATCAATG 
AAGCTGTGGT GTGCTGTTGG AGTCAATTTA TCTGGTGGGA AGACCAGAGA TGGTGGTTCT 
GTTGTTGGAG CAAGTGTATT TTACAAGGAT GTTGCTGGTT TGGATACAGA AGGCAGTAAA 
CAGCGAAGTG CCTCTCAGAG TAGTTTAGAT AAGTTAGATC AGGAACTTAA GGAACAGCAG 
AAGGAGTTAA AAAATCAAGA AGAATTATCC AGTCTAGTTT GGATCTGTAC CAGCACTCAT 
TCGGCTACAA AAGTTCTTAT TATTGATGCT GTTCAACCTG GCAACATCCT AGACAGTTTC 
ACTGTTTGCA ACTCTCATGT TCTGTGCATT GCAAGTGTGC CAGGTGCACG AGAAACAGAC 
TACCCTGCAG GAGAAGATCT TTCAGAATCT GGTCAGGTAG ACAAAGCATC TTTATGTGGA 
AGTATGACAA GCAACAGCTC AGCAGAGACA GACAGCCTGT TAGGAGGCAT CACAGTGGTT 
GGTTGTTCTG CAGAAGGTGT GACGGGAGCT GCCACTTCCC CTAGTACAAA TGGTGCTTCT 
CCAGTGATGG ATAAACCACC AGAAATGGAA GCAGAAAATA GTGAGGTTGA TGAAAATGTT 
CCAACAGCAG AAGAAGCAAC TGAAGCTACA GAAGGGAATG CGGGGTCAGC TGAAGACACA 
GTGGACATCT CCCAAACTGG CGTCTACACA GAGCATGTCT TTACAGATCC TTTGGGAGTT 
CAGATCCCAG AAGACCTCTC CCCAGTGTAT CAGTCGAGCA ATGACTCAGA TGCATATAAA 
GATCAAATAT CAGTACTGCC AAATGAACAA GACTTGGTGA GAGAAGAAGC CCAGAAAATG 
AGTAGTCTTT TACCAACTAT GTGGCTTGGA GCTCAAAATG GCTGTTTGTA TGTCCATTCA 
TCTGTAGCCC AGTGGAGGAA ATGTCTCCAT TCCATTAAAC TTAAAGATTC GATTCTCAGT 
ATTGTACACG TGAAGGGAAT CGTGTTAGTA GCCCTGGCTG ACGGCACCCT TGCAATCTTT 
CACAGAGGAG TGGATGGGCA GTGGGATTTG TCAAACTATC ACCTCTTAGA CCTTGGACGG 
CCTCATCATT CCATCCGTTG CATGACTGTG GTACATGACA AAGTCTGGTG TGGCTATAGG 
AACAAAATCT ATGTGGTGCA GCCAAAGGCC ATGAAAATAG AGAAATCTTT TGATGCACAT 
CCCAGGAAGG AGAGCCAAGT GCGACAGCTT GCGTGGGTGG GGGATGGCGT GTGGGTCTCC 
ATTCGCTTGG ATTCTACGCT CCGTCTCTAT CATGCACACA CTTATCAACA TCTACAGGAT 
GTGGACATTG AGCCTTATGT AAGCAAAATG TTAGGTACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATTA CAGCTCTTAT GGTGTCTTGT AATCGTTTGT GGGTGGGGAC AGGAAATGGT 
GTCATTATCT CCATCCCATT GACAGAAACC GTAATCCTCC ACCAGGGACG TTTACTGGGG 
CTGAGGGCAA ATAAAACCTC AGGTGTACCA GGAAATCGTC CTGGAAGTGT AATCCGTGTA 
TATGGTGATG AAAACAGTGA TAAAGTGACT CCAGGGACAT TTATACCCTA TTGTTCAATG 
GCACATGCAC AGCTTTGCTT CCATGGGCAC CGGGATGCTG TGAAATTCTT TGTGGCAGTC 
CCAGGTCAAG TCATCAGCCC ACAAAGTAGC AGTAGTGGCA CGGATCTGAC GGGTGACAAA 
GCAGGGCCAT CTGCACAGGA GCCTGGTAGT CAGACGCCCT TGAAGTCTAT GCTTGTCATC 
AGTGGAGGAG AGGGCTACAT CGACTTCCGA ATGGGTGATG AAGGTGGAGA ATCAGAACTT 
CTTGGAGAGG ATCTTCCACT TGAACCTTCT GTCACCAAAG CAGAAAGGAG TCACTTGATA 
GTGTGGCAAG TGATGTATGG CAATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_017025285.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880774.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu87606
Clone ID Related Accession (Same CDS sequence)	XM_017025285.2 , XM_017025285.1
Accession Version	XM_017025285.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCATA ATTATATGGA ACATTTAGAA AGAACAAAAC TTCATCAGCT CTCAGGGAGT 
GATCAACTAG AATCCACAGC TCATAGTAGA ATTAGAAAAG AACGCCCTAT ATCATTAGGA 
ATTTTCCCAT TACCTGCTGG AGATGGATTG CTTACACCTG ATGCTCAGAA AGGAGGAGAG 
ACCCCTGGAT CTGAGCAATG GAAATTTCAG GAATTAAGTC AACCACGTTC TCATACCAGC 
CTGAAGGTCA GCAATAGTCC TGAACCTCAG AAGGCTGTAG AACAGGAGGA TGAGCTTTCT 
GATGTTAGCC AAGGCGGATC TAAAGCTACC ACTCCAGCAT CAACAGCTAA TTCAGATGTG 
GCAACAATTC CTACTGATAC TCCCTTAAAG GAAGAAAACG AAGGATTTGT GAAGGTTACA 
GATGCGCCAA ATAAATCAGA GATAAGCAAA CACATTGAAG TACAGGTAGC CCAGGAAACT 
AGAAATGTAT CTACTGGCTC TGCTGAAAAT GAAGAAAAGT CAGAAGTTCA AGCAATCATC 
GAATCTACTC CTGAGCTGGA TATGGACAAA GATCTCAGTG GATATAAAGG TTCAAGCACT 
CCCACCAAAG GCATAGAGAA CAAAGCTTTT GATCGCAATA CAGAATCTCT CTTTGAAGAA 
CTGTCTTCAG CTGGCTCAGG CCTAATAGGA GATGTGGATG AAGGAGCAGA TTTACTAGGA 
ATGGGTCGGG AAGTTGAGAA TCTTATATTA GAAAATACAC AACTGTTGGA AACCAAAAAT 
GCTTTGAACA TAGTGAAGAA TGATTTGATA GCAAAAGTGG ATGAACTGAC CTGTGAGAAA 
GATGTGCTGC AAGGGGAATT GGAGGCTGTG AAGCAAGCCA AACTGAAACT AGAGGAAAAG 
AACAGAGAAT TGGAGGAAGA GCTTAGGAAA GCTCGGGCAG AAGCTGAAGA TGCAAGGCAA 
AAAGCAAAAG ATGACGATGA TAGTGATATT CCCACAGCCC AGAGGAAACG GTTTACTAGA 
GTAGAAATGG CCCGTGTTCT CATGGAGCGA AACCAGTATA AAGAGAGATT GATGGAGCTT 
CAGGAAGCTG TTCGATGGAC AGAGATGATT CGGGCATCAC GAGAAAATCC AGCCATGCAG 
GAAAAAAAAA GGTCAAGCAT TTGGCAGTTT TTCAGCCGAC TTTTCAGCTC CTCAAGTAAC 
ACGACTAAGA AGCCTGAACC ACCTGTTAAT CTGAAGTACA ATGCACCCAC GTCTCATGTT 
ACTCCGTCCG TCAAGAAAAG AAGCAGCACC TTATCTCAGC TCCCTGGGGA TAAGTCCAAA 
GCCTTTGATT TCCTTAGTGA AGAAACTGAA GCTAGTTTAG CCTCACGCAG AGAACAAAAG 
AGAGAGCAGT ATCGTCAGGT AAAAGCACAT GTTCAGAAGG AAGACGGTAG AGTGCAGGCT 
TTTGGCTGGA GTCTGCCTCA GAAGTACAAA CAGGTAACCA ATGGTCAAGG TGAAAATAAG 
ATGAAAAATT TACCTGTGCC TGTCTATCTC AGACCTCTGG ATGAAAAAGA TACATCAATG 
AAGCTGTGGT GTGCTGTTGG AGTCAATTTA TCTGGTGGGA AGACCAGAGA TGGTGGTTCT 
GTTGTTGGAG CAAGTGTATT TTACAAGGAT GTTGCTGGTT TGGATACAGA AGGCAGTAAA 
CAGCGAAGTG CCTCTCAGAG TAGTTTAGAT AAGTTAGATC AGGAACTTAA GGAACAGCAG 
AAGGAGTTAA AAAATCAAGA AGAATTATCC AGTCTAGTTT GGATCTGTAC CAGCACTCAT 
TCGGCTACAA AAGTTCTTAT TATTGATGCT GTTCAACCTG GCAACATCCT AGACAGTTTC 
ACTGTTTGCA ACTCTCATGT TCTGTGCATT GCAAGTGTGC CAGGTGCACG AGAAACAGAC 
TACCCTGCAG GAGAAGATCT TTCAGAATCT GGTCAGGTAG ACAAAGCATC TTTATGTGGA 
AGTATGACAA GCAACAGCTC AGCAGAGACA GACAGCCTGT TAGGAGGCAT CACAGTGGTT 
GGTTGTTCTG CAGAAGGTGT GACGGGAGCT GCCACTTCCC CTAGTACAAA TGGTGCTTCT 
CCAGTGATGG ATAAACCACC AGAAATGGAA GCAGAAAATA GTGAGGTTGA TGAAAATGTT 
CCAACAGCAG AAGAAGCAAC TGAAGCTACA GAAGGGAATG CGGGGTCAGC TGAAGACACA 
GTGGACATCT CCCAAACTGG CGTCTACACA GAGCATGTCT TTACAGATCC TTTGGGAGTT 
CAGATCCCAG AAGACCTCTC CCCAGTGTAT CAGTCGAGCA ATGACTCAGA TGCATATAAA 
GATCAAATAT CAGTACTGCC AAATGAACAA GACTTGGTGA GAGAAGAAGC CCAGAAAATG 
AGTAGTCTTT TACCAACTAT GTGGCTTGGA GCTCAAAATG GCTGTTTGTA TGTCCATTCA 
TCTGTAGCCC AGTGGAGGAA ATGTCTCCAT TCCATTAAAC TTAAAGATTC GATTCTCAGT 
ATTGTACACG TGAAGGGAAT CGTGTTAGTA GCCCTGGCTG ACGGCACCCT TGCAATCTTT 
CACAGAGGAG TGGATGGGCA GTGGGATTTG TCAAACTATC ACCTCTTAGA CCTTGGACGG 
CCTCATCATT CCATCCGTTG CATGACTGTG GTACATGACA AAGTCTGGTG TGGCTATAGG 
AACAAAATCT ATGTGGTGCA GCCAAAGGCC ATGAAAATAG AGAAATCTTT TGATGCACAT 
CCCAGGAAGG AGAGCCAAGT GCGACAGCTT GCGTGGGTGG GGGATGGCGT GTGGGTCTCC 
ATTCGCTTGG ATTCTACGCT CCGTCTCTAT CATGCACACA CTTATCAACA TCTACAGGAT 
GTGGACATTG AGCCTTATGT AAGCAAAATG TTAGGTACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATTA CAGCTCTTAT GGTGTCTTGT AATCGTTTGT GGGTGGGGAC AGGAAATGGT 
GTCATTATCT CCATCCCATT GACAGAAACC GTAATCCTCC ACCAGGGACG TTTACTGGGG 
CTGAGGGCAA ATAAAACCTC AGGTGTACCA GGAAATCGTC CTGGAAGTGT AATCCGTGTA 
TATGGTGATG AAAACAGTGA TAAAGTGACT CCAGGGACAT TTATACCCTA TTGTTCAATG 
GCACATGCAC AGCTTTGCTT CCATGGGCAC CGGGATGCTG TGAAATTCTT TGTGGCAGTC 
CCAGGTCAAG TCATCAGCCC ACAAAGTAGC AGTAGTGGCA CGGATCTGAC GGGTGACAAA 
GCAGGGCCAT CTGCACAGGA GCCTGGTAGT CAGACGCCCT TGAAGTCTAT GCTTGTCATC 
AGTGGAGGAG AGGGCTACAT CGACTTCCGA ATGGGTGATG AAGGTGGAGA ATCAGAACTT 
CTTGGAGAGG ATCTTCCACT TGAACCTTCT GTCACCAAAG CAGAAAGGAG TCACTTGATA 
GTGTGGCAAG TGATGTATGG CAATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880774.1
CDS	136..3645
Translation	MIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_017025285.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017025285.1

ORF Insert Sequence:

ATGATCCATA ATTATATGGA ACATTTAGAA AGAACAAAAC TTCATCAGCT CTCAGGGAGT 
GATCAACTAG AATCCACAGC TCATAGTAGA ATTAGAAAAG AACGCCCTAT ATCATTAGGA 
ATTTTCCCAT TACCTGCTGG AGATGGATTG CTTACACCTG ATGCTCAGAA AGGAGGAGAG 
ACCCCTGGAT CTGAGCAATG GAAATTTCAG GAATTAAGTC AACCACGTTC TCATACCAGC 
CTGAAGGTCA GCAATAGTCC TGAACCTCAG AAGGCTGTAG AACAGGAGGA TGAGCTTTCT 
GATGTTAGCC AAGGCGGATC TAAAGCTACC ACTCCAGCAT CAACAGCTAA TTCAGATGTG 
GCAACAATTC CTACTGATAC TCCCTTAAAG GAAGAAAACG AAGGATTTGT GAAGGTTACA 
GATGCGCCAA ATAAATCAGA GATAAGCAAA CACATTGAAG TACAGGTAGC CCAGGAAACT 
AGAAATGTAT CTACTGGCTC TGCTGAAAAT GAAGAAAAGT CAGAAGTTCA AGCAATCATC 
GAATCTACTC CTGAGCTGGA TATGGACAAA GATCTCAGTG GATATAAAGG TTCAAGCACT 
CCCACCAAAG GCATAGAGAA CAAAGCTTTT GATCGCAATA CAGAATCTCT CTTTGAAGAA 
CTGTCTTCAG CTGGCTCAGG CCTAATAGGA GATGTGGATG AAGGAGCAGA TTTACTAGGA 
ATGGGTCGGG AAGTTGAGAA TCTTATATTA GAAAATACAC AACTGTTGGA AACCAAAAAT 
GCTTTGAACA TAGTGAAGAA TGATTTGATA GCAAAAGTGG ATGAACTGAC CTGTGAGAAA 
GATGTGCTGC AAGGGGAATT GGAGGCTGTG AAGCAAGCCA AACTGAAACT AGAGGAAAAG 
AACAGAGAAT TGGAGGAAGA GCTTAGGAAA GCTCGGGCAG AAGCTGAAGA TGCAAGGCAA 
AAAGCAAAAG ATGACGATGA TAGTGATATT CCCACAGCCC AGAGGAAACG GTTTACTAGA 
GTAGAAATGG CCCGTGTTCT CATGGAGCGA AACCAGTATA AAGAGAGATT GATGGAGCTT 
CAGGAAGCTG TTCGATGGAC AGAGATGATT CGGGCATCAC GAGAAAATCC AGCCATGCAG 
GAAAAAAAAA GGTCAAGCAT TTGGCAGTTT TTCAGCCGAC TTTTCAGCTC CTCAAGTAAC 
ACGACTAAGA AGCCTGAACC ACCTGTTAAT CTGAAGTACA ATGCACCCAC GTCTCATGTT 
ACTCCGTCCG TCAAGAAAAG AAGCAGCACC TTATCTCAGC TCCCTGGGGA TAAGTCCAAA 
GCCTTTGATT TCCTTAGTGA AGAAACTGAA GCTAGTTTAG CCTCACGCAG AGAACAAAAG 
AGAGAGCAGT ATCGTCAGGT AAAAGCACAT GTTCAGAAGG AAGACGGTAG AGTGCAGGCT 
TTTGGCTGGA GTCTGCCTCA GAAGTACAAA CAGGTAACCA ATGGTCAAGG TGAAAATAAG 
ATGAAAAATT TACCTGTGCC TGTCTATCTC AGACCTCTGG ATGAAAAAGA TACATCAATG 
AAGCTGTGGT GTGCTGTTGG AGTCAATTTA TCTGGTGGGA AGACCAGAGA TGGTGGTTCT 
GTTGTTGGAG CAAGTGTATT TTACAAGGAT GTTGCTGGTT TGGATACAGA AGGCAGTAAA 
CAGCGAAGTG CCTCTCAGAG TAGTTTAGAT AAGTTAGATC AGGAACTTAA GGAACAGCAG 
AAGGAGTTAA AAAATCAAGA AGAATTATCC AGTCTAGTTT GGATCTGTAC CAGCACTCAT 
TCGGCTACAA AAGTTCTTAT TATTGATGCT GTTCAACCTG GCAACATCCT AGACAGTTTC 
ACTGTTTGCA ACTCTCATGT TCTGTGCATT GCAAGTGTGC CAGGTGCACG AGAAACAGAC 
TACCCTGCAG GAGAAGATCT TTCAGAATCT GGTCAGGTAG ACAAAGCATC TTTATGTGGA 
AGTATGACAA GCAACAGCTC AGCAGAGACA GACAGCCTGT TAGGAGGCAT CACAGTGGTT 
GGTTGTTCTG CAGAAGGTGT GACGGGAGCT GCCACTTCCC CTAGTACAAA TGGTGCTTCT 
CCAGTGATGG ATAAACCACC AGAAATGGAA GCAGAAAATA GTGAGGTTGA TGAAAATGTT 
CCAACAGCAG AAGAAGCAAC TGAAGCTACA GAAGGGAATG CGGGGTCAGC TGAAGACACA 
GTGGACATCT CCCAAACTGG CGTCTACACA GAGCATGTCT TTACAGATCC TTTGGGAGTT 
CAGATCCCAG AAGACCTCTC CCCAGTGTAT CAGTCGAGCA ATGACTCAGA TGCATATAAA 
GATCAAATAT CAGTACTGCC AAATGAACAA GACTTGGTGA GAGAAGAAGC CCAGAAAATG 
AGTAGTCTTT TACCAACTAT GTGGCTTGGA GCTCAAAATG GCTGTTTGTA TGTCCATTCA 
TCTGTAGCCC AGTGGAGGAA ATGTCTCCAT TCCATTAAAC TTAAAGATTC GATTCTCAGT 
ATTGTACACG TGAAGGGAAT CGTGTTAGTA GCCCTGGCTG ACGGCACCCT TGCAATCTTT 
CACAGAGGAG TGGATGGGCA GTGGGATTTG TCAAACTATC ACCTCTTAGA CCTTGGACGG 
CCTCATCATT CCATCCGTTG CATGACTGTG GTACATGACA AAGTCTGGTG TGGCTATAGG 
AACAAAATCT ATGTGGTGCA GCCAAAGGCC ATGAAAATAG AGAAATCTTT TGATGCACAT 
CCCAGGAAGG AGAGCCAAGT GCGACAGCTT GCGTGGGTGG GGGATGGCGT GTGGGTCTCC 
ATTCGCTTGG ATTCTACGCT CCGTCTCTAT CATGCACACA CTTATCAACA TCTACAGGAT 
GTGGACATTG AGCCTTATGT AAGCAAAATG TTAGGTACTG GAAAACTGGG CTTCTCTTTT 
GTGAGAATTA CAGCTCTTAT GGTGTCTTGT AATCGTTTGT GGGTGGGGAC AGGAAATGGT 
GTCATTATCT CCATCCCATT GACAGAAACC GTAATCCTCC ACCAGGGACG TTTACTGGGG 
CTGAGGGCAA ATAAAACCTC AGGTGTACCA GGAAATCGTC CTGGAAGTGT AATCCGTGTA 
TATGGTGATG AAAACAGTGA TAAAGTGACT CCAGGGACAT TTATACCCTA TTGTTCAATG 
GCACATGCAC AGCTTTGCTT CCATGGGCAC CGGGATGCTG TGAAATTCTT TGTGGCAGTC 
CCAGGTCAAG TCATCAGCCC ACAAAGTAGC AGTAGTGGCA CGGATCTGAC GGGTGACAAA 
GCAGGGCCAT CTGCACAGGA GCCTGGTAGT CAGACGCCCT TGAAGTCTAT GCTTGTCATC 
AGTGGAGGAG AGGGCTACAT CGACTTCCGA ATGGGTGATG AAGGTGGAGA ATCAGAACTT 
CTTGGAGAGG ATCTTCCACT TGAACCTTCT GTCACCAAAG CAGAAAGGAG TCACTTGATA 
GTGTGGCAAG TGATGTATGG CAATGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_001251971.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238900.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 4, mRNA.

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CloneID	OHu28428
Clone ID Related Accession (Same CDS sequence)	NM_001251971.1 , NM_001251971.2
Accession Version	NM_001251971.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC332810.1, AK295098.1 and AC005920.1. Transcript Variant: This variant (4) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. The resulting isoform (4) has a distinct N-terminus and is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.238016.1, SRR1660809.169783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACCGTAATC 
CTCCACCAGG GACGTTTACT GGGGCTGAGG GCAAATAAAA CCTCAGGTGT ACCAGGAAAT 
CGTCCTGGAA GTGTAATCCG TGTATATGGT GATGAAAACA GTGATAAAGT GACTCCAGGG 
ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT GCTTCCATGG GCACCGGGAT 
GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT CAAGTCATCA GCCCACAAAG TAGCAGTAGT 
GGCACGGATC TGACGGGTGA CAAAGCAGGG CCATCTGCAC AGGAGCCTGG TAGTCAGACG 
CCCTTGAAGT CTATGCTTGT CATCAGTGGA GGAGAGGGCT ACATCGACTT CCGAATGGGT 
GATGAAGGTG GAGAATCAGA ACTTCTTGGA GAGGATCTTC CACTTGAACC TTCTGTCACC 
AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238900.1
CDS	309..3842
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_001251971.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251971.1

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACCGTAATC 
CTCCACCAGG GACGTTTACT GGGGCTGAGG GCAAATAAAA CCTCAGGTGT ACCAGGAAAT 
CGTCCTGGAA GTGTAATCCG TGTATATGGT GATGAAAACA GTGATAAAGT GACTCCAGGG 
ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT GCTTCCATGG GCACCGGGAT 
GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT CAAGTCATCA GCCCACAAAG TAGCAGTAGT 
GGCACGGATC TGACGGGTGA CAAAGCAGGG CCATCTGCAC AGGAGCCTGG TAGTCAGACG 
CCCTTGAAGT CTATGCTTGT CATCAGTGGA GGAGAGGGCT ACATCGACTT CCGAATGGGT 
GATGAAGGTG GAGAATCAGA ACTTCTTGGA GAGGATCTTC CACTTGAACC TTCTGTCACC 
AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_001251971.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001238900.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 4, mRNA.

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CloneID	OHu28428
Clone ID Related Accession (Same CDS sequence)	NM_001251971.1 , NM_001251971.2
Accession Version	NM_001251971.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3534bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC332810.1, AK295098.1 and AC005920.1. On May 31, 2019 this sequence version replaced NM_001251971.1. Transcript Variant: This variant (4) differs in the 5' UTR and has multiple coding region differences, compared to variant 1. The resulting isoform (4) has a distinct N-terminus and is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK295098.1, SRR1660809.169783.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACCGTAATC 
CTCCACCAGG GACGTTTACT GGGGCTGAGG GCAAATAAAA CCTCAGGTGT ACCAGGAAAT 
CGTCCTGGAA GTGTAATCCG TGTATATGGT GATGAAAACA GTGATAAAGT GACTCCAGGG 
ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT GCTTCCATGG GCACCGGGAT 
GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT CAAGTCATCA GCCCACAAAG TAGCAGTAGT 
GGCACGGATC TGACGGGTGA CAAAGCAGGG CCATCTGCAC AGGAGCCTGG TAGTCAGACG 
CCCTTGAAGT CTATGCTTGT CATCAGTGGA GGAGAGGGCT ACATCGACTT CCGAATGGGT 
GATGAAGGTG GAGAATCAGA ACTTCTTGGA GAGGATCTTC CACTTGAACC TTCTGTCACC 
AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001238900.1
CDS	219..3752
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_001251971.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001251971.2

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACCGTAATC 
CTCCACCAGG GACGTTTACT GGGGCTGAGG GCAAATAAAA CCTCAGGTGT ACCAGGAAAT 
CGTCCTGGAA GTGTAATCCG TGTATATGGT GATGAAAACA GTGATAAAGT GACTCCAGGG 
ACATTTATAC CCTATTGTTC AATGGCACAT GCACAGCTTT GCTTCCATGG GCACCGGGAT 
GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT CAAGTCATCA GCCCACAAAG TAGCAGTAGT 
GGCACGGATC TGACGGGTGA CAAAGCAGGG CCATCTGCAC AGGAGCCTGG TAGTCAGACG 
CCCTTGAAGT CTATGCTTGT CATCAGTGGA GGAGAGGGCT ACATCGACTT CCGAATGGGT 
GATGAAGGTG GAGAATCAGA ACTTCTTGGA GAGGATCTTC CACTTGAACC TTCTGTCACC 
AAAGCAGAAA GGAGTCACTT GATAGTGTGG CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_003971.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003962.3 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28436
Clone ID Related Accession (Same CDS sequence)	NM_003971.5 , NM_003971.6
Accession Version	NM_003971.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK091921.1, AY850123.1 and AC005920.1. On Aug 6, 2008 this sequence version replaced NM_003971.4. Transcript Variant: This variant (3) lacks an in-frame coding exon in the coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY850123.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACAAATAAAA CCTCAGGTGT ACCAGGAAAT CGTCCTGGAA GTGTAATCCG TGTATATGGT 
GATGAAAACA GTGATAAAGT GACTCCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT 
GCACAGCTTT GCTTCCATGG GCACCGGGAT GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT 
CAAGTCATCA GCCCACAAAG TAGCAGTAGT GGCACGGATC TGACGGGTGA CAAAGCAGGG 
CCATCTGCAC AGGAGCCTGG TAGTCAGACG CCCTTGAAGT CTATGCTTGT CATCAGTGGA 
GGAGAGGGCT ACATCGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA ACTTCTTGGA 
GAGGATCTTC CACTTGAACC TTCTGTCACC AAAGCAGAAA GGAGTCACTT GATAGTGTGG 
CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003962.3
CDS	210..4133
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_003971.5
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003971.5

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACAAATAAAA CCTCAGGTGT ACCAGGAAAT CGTCCTGGAA GTGTAATCCG TGTATATGGT 
GATGAAAACA GTGATAAAGT GACTCCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT 
GCACAGCTTT GCTTCCATGG GCACCGGGAT GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT 
CAAGTCATCA GCCCACAAAG TAGCAGTAGT GGCACGGATC TGACGGGTGA CAAAGCAGGG 
CCATCTGCAC AGGAGCCTGG TAGTCAGACG CCCTTGAAGT CTATGCTTGT CATCAGTGGA 
GGAGAGGGCT ACATCGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA ACTTCTTGGA 
GAGGATCTTC CACTTGAACC TTCTGTCACC AAAGCAGAAA GGAGTCACTT GATAGTGTGG 
CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_003971.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_003962.3 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 3, mRNA.

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CloneID	OHu28436
Clone ID Related Accession (Same CDS sequence)	NM_003971.5 , NM_003971.6
Accession Version	NM_003971.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005839.1, AK091921.1, AY850123.1 and AC005920.1. On May 31, 2019 this sequence version replaced NM_003971.5. Transcript Variant: This variant (3) lacks an in-frame coding exon in the coding region, compared to variant 1. The resulting isoform (3) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY850123.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACAAATAAAA CCTCAGGTGT ACCAGGAAAT CGTCCTGGAA GTGTAATCCG TGTATATGGT 
GATGAAAACA GTGATAAAGT GACTCCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT 
GCACAGCTTT GCTTCCATGG GCACCGGGAT GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT 
CAAGTCATCA GCCCACAAAG TAGCAGTAGT GGCACGGATC TGACGGGTGA CAAAGCAGGG 
CCATCTGCAC AGGAGCCTGG TAGTCAGACG CCCTTGAAGT CTATGCTTGT CATCAGTGGA 
GGAGAGGGCT ACATCGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA ACTTCTTGGA 
GAGGATCTTC CACTTGAACC TTCTGTCACC AAAGCAGAAA GGAGTCACTT GATAGTGTGG 
CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003962.3
CDS	213..4136
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_003971.6
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003971.6

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACAAATAAAA CCTCAGGTGT ACCAGGAAAT CGTCCTGGAA GTGTAATCCG TGTATATGGT 
GATGAAAACA GTGATAAAGT GACTCCAGGG ACATTTATAC CCTATTGTTC AATGGCACAT 
GCACAGCTTT GCTTCCATGG GCACCGGGAT GCTGTGAAAT TCTTTGTGGC AGTCCCAGGT 
CAAGTCATCA GCCCACAAAG TAGCAGTAGT GGCACGGATC TGACGGGTGA CAAAGCAGGG 
CCATCTGCAC AGGAGCCTGG TAGTCAGACG CCCTTGAAGT CTATGCTTGT CATCAGTGGA 
GGAGAGGGCT ACATCGACTT CCGAATGGGT GATGAAGGTG GAGAATCAGA ACTTCTTGGA 
GAGGATCTTC CACTTGAACC TTCTGTCACC AAAGCAGAAA GGAGTCACTT GATAGTGTGG 
CAAGTGATGT ATGGCAATGA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_001130527.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123999.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28461
Clone ID Related Accession (Same CDS sequence)	NM_001130527.3 , NM_001130527.2
Accession Version	NM_001130527.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC005839.1, DA631864.1, AF327452.1, AY236487.1 and AC005920.1. On May 31, 2019 this sequence version replaced NM_001130527.2. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF327452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTGTGCCAA CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACGAC TAAGAAGCCT 
GAACCACCTG TTAATCTGAA GTACAATGCA CCCACGTCTC ATGTTACTCC GTCCGTCAAG 
AAAAGAAGCA GCACCTTATC TCAGCTCCCT GGGGATAAGT CCAAAGCCTT TGATTTCCTT 
AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA CGCAGAGAAC AAAAGAGAGA GCAGTATCGT 
CAGGTAAAAG CACATGTTCA GAAGGAAGAC GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG 
CCTCAGAAGT ACAAACAGGT AACCAATGGT CAAGGTGAAA ATAAGATGAA AAATTTACCT 
GTGCCTGTCT ATCTCAGACC TCTGGATGAA AAAGATACAT CAATGAAGCT GTGGTGTGCT 
GTTGGAGTCA ATTTATCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT 
GTATTTTACA AGGATGTTGC TGGTTTGGAT ACAGAAGGCA GTAAACAGCG AAGTGCCTCT 
CAGAGTAGTT TAGATAAGTT AGATCAGGAA CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT 
CAAGAAGAAT TATCCAGTCT AGTTTGGATC TGTACCAGCA CTCATTCGGC TACAAAAGTT 
CTTATTATTG ATGCTGTTCA ACCTGGCAAC ATCCTAGACA GTTTCACTGT TTGCAACTCT 
CATGTTCTGT GCATTGCAAG TGTGCCAGGT GCACGAGAAA CAGACTACCC TGCAGGAGAA 
GATCTTTCAG AATCTGGTCA GGTAGACAAA GCATCTTTAT GTGGAAGTAT GACAAGCAAC 
AGCTCAGCAG AGACAGACAG CCTGTTAGGA GGCATCACAG TGGTTGGTTG TTCTGCAGAA 
GGTGTGACGG GAGCTGCCAC TTCCCCTAGT ACAAATGGTG CTTCTCCAGT GATGGATAAA 
CCACCAGAAA TGGAAGCAGA AAATAGTGAG GTTGATGAAA ATGTTCCAAC AGCAGAAGAA 
GCAACTGAAG CTACAGAAGG GAATGCGGGG TCAGCTGAAG ACACAGTGGA CATCTCCCAA 
ACTGGCGTCT ACACAGAGCA TGTCTTTACA GATCCTTTGG GAGTTCAGAT CCCAGAAGAC 
CTCTCCCCAG TGTATCAGTC GAGCAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA 
CTGCCAAATG AACAAGACTT GGTGAGAGAA GAAGCCCAGA AAATGAGTAG TCTTTTACCA 
ACTATGTGGC TTGGAGCTCA AAATGGCTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG 
AGGAAATGTC TCCATTCCAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAG 
GGAATCGTGT TAGTAGCCCT GGCTGACGGC ACCCTTGCAA TCTTTCACAG AGGAGTGGAT 
GGGCAGTGGG ATTTGTCAAA CTATCACCTC TTAGACCTTG GACGGCCTCA TCATTCCATC 
CGTTGCATGA CTGTGGTACA TGACAAAGTC TGGTGTGGCT ATAGGAACAA AATCTATGTG 
GTGCAGCCAA AGGCCATGAA AATAGAGAAA TCTTTTGATG CACATCCCAG GAAGGAGAGC 
CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT GGCGTGTGGG TCTCCATTCG CTTGGATTCT 
ACGCTCCGTC TCTATCATGC ACACACTTAT CAACATCTAC AGGATGTGGA CATTGAGCCT 
TATGTAAGCA AAATGTTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG AATTACAGCT 
CTTATGGTGT CTTGTAATCG TTTGTGGGTG GGGACAGGAA ATGGTGTCAT TATCTCCATC 
CCATTGACAG AAACAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123999.1
CDS	213..4148
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFVPTRFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_001130527.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130527.3

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTGTGCCAA CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACGAC TAAGAAGCCT 
GAACCACCTG TTAATCTGAA GTACAATGCA CCCACGTCTC ATGTTACTCC GTCCGTCAAG 
AAAAGAAGCA GCACCTTATC TCAGCTCCCT GGGGATAAGT CCAAAGCCTT TGATTTCCTT 
AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA CGCAGAGAAC AAAAGAGAGA GCAGTATCGT 
CAGGTAAAAG CACATGTTCA GAAGGAAGAC GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG 
CCTCAGAAGT ACAAACAGGT AACCAATGGT CAAGGTGAAA ATAAGATGAA AAATTTACCT 
GTGCCTGTCT ATCTCAGACC TCTGGATGAA AAAGATACAT CAATGAAGCT GTGGTGTGCT 
GTTGGAGTCA ATTTATCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT 
GTATTTTACA AGGATGTTGC TGGTTTGGAT ACAGAAGGCA GTAAACAGCG AAGTGCCTCT 
CAGAGTAGTT TAGATAAGTT AGATCAGGAA CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT 
CAAGAAGAAT TATCCAGTCT AGTTTGGATC TGTACCAGCA CTCATTCGGC TACAAAAGTT 
CTTATTATTG ATGCTGTTCA ACCTGGCAAC ATCCTAGACA GTTTCACTGT TTGCAACTCT 
CATGTTCTGT GCATTGCAAG TGTGCCAGGT GCACGAGAAA CAGACTACCC TGCAGGAGAA 
GATCTTTCAG AATCTGGTCA GGTAGACAAA GCATCTTTAT GTGGAAGTAT GACAAGCAAC 
AGCTCAGCAG AGACAGACAG CCTGTTAGGA GGCATCACAG TGGTTGGTTG TTCTGCAGAA 
GGTGTGACGG GAGCTGCCAC TTCCCCTAGT ACAAATGGTG CTTCTCCAGT GATGGATAAA 
CCACCAGAAA TGGAAGCAGA AAATAGTGAG GTTGATGAAA ATGTTCCAAC AGCAGAAGAA 
GCAACTGAAG CTACAGAAGG GAATGCGGGG TCAGCTGAAG ACACAGTGGA CATCTCCCAA 
ACTGGCGTCT ACACAGAGCA TGTCTTTACA GATCCTTTGG GAGTTCAGAT CCCAGAAGAC 
CTCTCCCCAG TGTATCAGTC GAGCAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA 
CTGCCAAATG AACAAGACTT GGTGAGAGAA GAAGCCCAGA AAATGAGTAG TCTTTTACCA 
ACTATGTGGC TTGGAGCTCA AAATGGCTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG 
AGGAAATGTC TCCATTCCAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAG 
GGAATCGTGT TAGTAGCCCT GGCTGACGGC ACCCTTGCAA TCTTTCACAG AGGAGTGGAT 
GGGCAGTGGG ATTTGTCAAA CTATCACCTC TTAGACCTTG GACGGCCTCA TCATTCCATC 
CGTTGCATGA CTGTGGTACA TGACAAAGTC TGGTGTGGCT ATAGGAACAA AATCTATGTG 
GTGCAGCCAA AGGCCATGAA AATAGAGAAA TCTTTTGATG CACATCCCAG GAAGGAGAGC 
CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT GGCGTGTGGG TCTCCATTCG CTTGGATTCT 
ACGCTCCGTC TCTATCATGC ACACACTTAT CAACATCTAC AGGATGTGGA CATTGAGCCT 
TATGTAAGCA AAATGTTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG AATTACAGCT 
CTTATGGTGT CTTGTAATCG TTTGTGGGTG GGGACAGGAA ATGGTGTCAT TATCTCCATC 
CCATTGACAG AAACAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( NM_001130527.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123999.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu28461
Clone ID Related Accession (Same CDS sequence)	NM_001130527.3 , NM_001130527.2
Accession Version	NM_001130527.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA631864.1, AF327452.1, AY236487.1 and AC005920.1. On Oct 28, 2011 this sequence version replaced NM_001130527.1. Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF327452.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTGTGCCAA CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACGAC TAAGAAGCCT 
GAACCACCTG TTAATCTGAA GTACAATGCA CCCACGTCTC ATGTTACTCC GTCCGTCAAG 
AAAAGAAGCA GCACCTTATC TCAGCTCCCT GGGGATAAGT CCAAAGCCTT TGATTTCCTT 
AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA CGCAGAGAAC AAAAGAGAGA GCAGTATCGT 
CAGGTAAAAG CACATGTTCA GAAGGAAGAC GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG 
CCTCAGAAGT ACAAACAGGT AACCAATGGT CAAGGTGAAA ATAAGATGAA AAATTTACCT 
GTGCCTGTCT ATCTCAGACC TCTGGATGAA AAAGATACAT CAATGAAGCT GTGGTGTGCT 
GTTGGAGTCA ATTTATCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT 
GTATTTTACA AGGATGTTGC TGGTTTGGAT ACAGAAGGCA GTAAACAGCG AAGTGCCTCT 
CAGAGTAGTT TAGATAAGTT AGATCAGGAA CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT 
CAAGAAGAAT TATCCAGTCT AGTTTGGATC TGTACCAGCA CTCATTCGGC TACAAAAGTT 
CTTATTATTG ATGCTGTTCA ACCTGGCAAC ATCCTAGACA GTTTCACTGT TTGCAACTCT 
CATGTTCTGT GCATTGCAAG TGTGCCAGGT GCACGAGAAA CAGACTACCC TGCAGGAGAA 
GATCTTTCAG AATCTGGTCA GGTAGACAAA GCATCTTTAT GTGGAAGTAT GACAAGCAAC 
AGCTCAGCAG AGACAGACAG CCTGTTAGGA GGCATCACAG TGGTTGGTTG TTCTGCAGAA 
GGTGTGACGG GAGCTGCCAC TTCCCCTAGT ACAAATGGTG CTTCTCCAGT GATGGATAAA 
CCACCAGAAA TGGAAGCAGA AAATAGTGAG GTTGATGAAA ATGTTCCAAC AGCAGAAGAA 
GCAACTGAAG CTACAGAAGG GAATGCGGGG TCAGCTGAAG ACACAGTGGA CATCTCCCAA 
ACTGGCGTCT ACACAGAGCA TGTCTTTACA GATCCTTTGG GAGTTCAGAT CCCAGAAGAC 
CTCTCCCCAG TGTATCAGTC GAGCAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA 
CTGCCAAATG AACAAGACTT GGTGAGAGAA GAAGCCCAGA AAATGAGTAG TCTTTTACCA 
ACTATGTGGC TTGGAGCTCA AAATGGCTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG 
AGGAAATGTC TCCATTCCAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAG 
GGAATCGTGT TAGTAGCCCT GGCTGACGGC ACCCTTGCAA TCTTTCACAG AGGAGTGGAT 
GGGCAGTGGG ATTTGTCAAA CTATCACCTC TTAGACCTTG GACGGCCTCA TCATTCCATC 
CGTTGCATGA CTGTGGTACA TGACAAAGTC TGGTGTGGCT ATAGGAACAA AATCTATGTG 
GTGCAGCCAA AGGCCATGAA AATAGAGAAA TCTTTTGATG CACATCCCAG GAAGGAGAGC 
CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT GGCGTGTGGG TCTCCATTCG CTTGGATTCT 
ACGCTCCGTC TCTATCATGC ACACACTTAT CAACATCTAC AGGATGTGGA CATTGAGCCT 
TATGTAAGCA AAATGTTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG AATTACAGCT 
CTTATGGTGT CTTGTAATCG TTTGTGGGTG GGGACAGGAA ATGGTGTCAT TATCTCCATC 
CCATTGACAG AAACAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123999.1
CDS	210..4145
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFVPTRFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	NM_001130527.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130527.2

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTGTGCCAA CTCGTTTCAG CCGACTTTTC AGCTCCTCAA GTAACACGAC TAAGAAGCCT 
GAACCACCTG TTAATCTGAA GTACAATGCA CCCACGTCTC ATGTTACTCC GTCCGTCAAG 
AAAAGAAGCA GCACCTTATC TCAGCTCCCT GGGGATAAGT CCAAAGCCTT TGATTTCCTT 
AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA CGCAGAGAAC AAAAGAGAGA GCAGTATCGT 
CAGGTAAAAG CACATGTTCA GAAGGAAGAC GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG 
CCTCAGAAGT ACAAACAGGT AACCAATGGT CAAGGTGAAA ATAAGATGAA AAATTTACCT 
GTGCCTGTCT ATCTCAGACC TCTGGATGAA AAAGATACAT CAATGAAGCT GTGGTGTGCT 
GTTGGAGTCA ATTTATCTGG TGGGAAGACC AGAGATGGTG GTTCTGTTGT TGGAGCAAGT 
GTATTTTACA AGGATGTTGC TGGTTTGGAT ACAGAAGGCA GTAAACAGCG AAGTGCCTCT 
CAGAGTAGTT TAGATAAGTT AGATCAGGAA CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT 
CAAGAAGAAT TATCCAGTCT AGTTTGGATC TGTACCAGCA CTCATTCGGC TACAAAAGTT 
CTTATTATTG ATGCTGTTCA ACCTGGCAAC ATCCTAGACA GTTTCACTGT TTGCAACTCT 
CATGTTCTGT GCATTGCAAG TGTGCCAGGT GCACGAGAAA CAGACTACCC TGCAGGAGAA 
GATCTTTCAG AATCTGGTCA GGTAGACAAA GCATCTTTAT GTGGAAGTAT GACAAGCAAC 
AGCTCAGCAG AGACAGACAG CCTGTTAGGA GGCATCACAG TGGTTGGTTG TTCTGCAGAA 
GGTGTGACGG GAGCTGCCAC TTCCCCTAGT ACAAATGGTG CTTCTCCAGT GATGGATAAA 
CCACCAGAAA TGGAAGCAGA AAATAGTGAG GTTGATGAAA ATGTTCCAAC AGCAGAAGAA 
GCAACTGAAG CTACAGAAGG GAATGCGGGG TCAGCTGAAG ACACAGTGGA CATCTCCCAA 
ACTGGCGTCT ACACAGAGCA TGTCTTTACA GATCCTTTGG GAGTTCAGAT CCCAGAAGAC 
CTCTCCCCAG TGTATCAGTC GAGCAATGAC TCAGATGCAT ATAAAGATCA AATATCAGTA 
CTGCCAAATG AACAAGACTT GGTGAGAGAA GAAGCCCAGA AAATGAGTAG TCTTTTACCA 
ACTATGTGGC TTGGAGCTCA AAATGGCTGT TTGTATGTCC ATTCATCTGT AGCCCAGTGG 
AGGAAATGTC TCCATTCCAT TAAACTTAAA GATTCGATTC TCAGTATTGT ACACGTGAAG 
GGAATCGTGT TAGTAGCCCT GGCTGACGGC ACCCTTGCAA TCTTTCACAG AGGAGTGGAT 
GGGCAGTGGG ATTTGTCAAA CTATCACCTC TTAGACCTTG GACGGCCTCA TCATTCCATC 
CGTTGCATGA CTGTGGTACA TGACAAAGTC TGGTGTGGCT ATAGGAACAA AATCTATGTG 
GTGCAGCCAA AGGCCATGAA AATAGAGAAA TCTTTTGATG CACATCCCAG GAAGGAGAGC 
CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT GGCGTGTGGG TCTCCATTCG CTTGGATTCT 
ACGCTCCGTC TCTATCATGC ACACACTTAT CAACATCTAC AGGATGTGGA CATTGAGCCT 
TATGTAAGCA AAATGTTAGG TACTGGAAAA CTGGGCTTCT CTTTTGTGAG AATTACAGCT 
CTTATGGTGT CTTGTAATCG TTTGTGGGTG GGGACAGGAA ATGGTGTCAT TATCTCCATC 
CCATTGACAG AAACAAATAA AACCTCAGGT GTACCAGGAA ATCGTCCTGG AAGTGTAATC 
CGTGTATATG GTGATGAAAA CAGTGATAAA GTGACTCCAG GGACATTTAT ACCCTATTGT 
TCAATGGCAC ATGCACAGCT TTGCTTCCAT GGGCACCGGG ATGCTGTGAA ATTCTTTGTG 
GCAGTCCCAG GTCAAGTCAT CAGCCCACAA AGTAGCAGTA GTGGCACGGA TCTGACGGGT 
GACAAAGCAG GGCCATCTGC ACAGGAGCCT GGTAGTCAGA CGCCCTTGAA GTCTATGCTT 
GTCATCAGTG GAGGAGAGGG CTACATCGAC TTCCGAATGG GTGATGAAGG TGGAGAATCA 
GAACTTCTTG GAGAGGATCT TCCACTTGAA CCTTCTGTCA CCAAAGCAGA AAGGAGTCAC 
TTGATAGTGT GGCAAGTGAT GTATGGCAAT GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_005257771.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257828.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.

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CloneID	OHu50770
Clone ID Related Accession (Same CDS sequence)	XM_005257771.2 , XM_005257771.3
Accession Version	XM_005257771.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CAAATAAAAC CTCAGGTGTA 
CCAGGAAATC GTCCTGGAAG TGTAATCCGT GTATATGGTG ATGAAAACAG TGATAAAGTG 
ACTCCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG 
CACCGGGATG CTGTGAAATT CTTTGTGGCA GTCCCAGGTC AAGTCATCAG CCCACAAAGT 
AGCAGTAGTG GCACGGATCT GACGGGTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGT 
AGTCAGACGC CCTTGAAGTC TATGCTTGTC ATCAGTGGAG GAGAGGGCTA CATCGACTTC 
CGAATGGGTG ATGAAGGTGG AGAATCAGAA CTTCTTGGAG AGGATCTTCC ACTTGAACCT 
TCTGTCACCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGCAATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257828.1
CDS	210..4172
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_005257771.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257771.2

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CAAATAAAAC CTCAGGTGTA 
CCAGGAAATC GTCCTGGAAG TGTAATCCGT GTATATGGTG ATGAAAACAG TGATAAAGTG 
ACTCCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG 
CACCGGGATG CTGTGAAATT CTTTGTGGCA GTCCCAGGTC AAGTCATCAG CCCACAAAGT 
AGCAGTAGTG GCACGGATCT GACGGGTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGT 
AGTCAGACGC CCTTGAAGTC TATGCTTGTC ATCAGTGGAG GAGAGGGCTA CATCGACTTC 
CGAATGGGTG ATGAAGGTGG AGAATCAGAA CTTCTTGGAG AGGATCTTCC ACTTGAACCT 
TCTGTCACCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGCAATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_005257771.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257828.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu50770
Clone ID Related Accession (Same CDS sequence)	XM_005257771.2 , XM_005257771.3
Accession Version	XM_005257771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3963bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005257771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CAAATAAAAC CTCAGGTGTA 
CCAGGAAATC GTCCTGGAAG TGTAATCCGT GTATATGGTG ATGAAAACAG TGATAAAGTG 
ACTCCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG 
CACCGGGATG CTGTGAAATT CTTTGTGGCA GTCCCAGGTC AAGTCATCAG CCCACAAAGT 
AGCAGTAGTG GCACGGATCT GACGGGTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGT 
AGTCAGACGC CCTTGAAGTC TATGCTTGTC ATCAGTGGAG GAGAGGGCTA CATCGACTTC 
CGAATGGGTG ATGAAGGTGG AGAATCAGAA CTTCTTGGAG AGGATCTTCC ACTTGAACCT 
TCTGTCACCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGCAATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257828.1
CDS	210..4172
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_005257771.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257771.3

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGATGAGCTT TCTGATGTTA GCCAAGGCGG ATCTAAAGCT 
ACCACTCCAG CATCAACAGC TAATTCAGAT GTGGCAACAA TTCCTACTGA TACTCCCTTA 
AAGGAAGAAA ACGAAGGATT TGTGAAGGTT ACAGATGCGC CAAATAAATC AGAGATAAGC 
AAACACATTG AAGTACAGGT AGCCCAGGAA ACTAGAAATG TATCTACTGG CTCTGCTGAA 
AATGAAGAAA AGTCAGAAGT TCAAGCAATC ATCGAATCTA CTCCTGAGCT GGATATGGAC 
AAAGATCTCA GTGGATATAA AGGTTCAAGC ACTCCCACCA AAGGCATAGA GAACAAAGCT 
TTTGATCGCA ATACAGAATC TCTCTTTGAA GAACTGTCTT CAGCTGGCTC AGGCCTAATA 
GGAGATGTGG ATGAAGGAGC AGATTTACTA GGAATGGGTC GGGAAGTTGA GAATCTTATA 
TTAGAAAATA CACAACTGTT GGAAACCAAA AATGCTTTGA ACATAGTGAA GAATGATTTG 
ATAGCAAAAG TGGATGAACT GACCTGTGAG AAAGATGTGC TGCAAGGGGA ATTGGAGGCT 
GTGAAGCAAG CCAAACTGAA ACTAGAGGAA AAGAACAGAG AATTGGAGGA AGAGCTTAGG 
AAAGCTCGGG CAGAAGCTGA AGATGCAAGG CAAAAAGCAA AAGATGACGA TGATAGTGAT 
ATTCCCACAG CCCAGAGGAA ACGGTTTACT AGAGTAGAAA TGGCCCGTGT TCTCATGGAG 
CGAAACCAGT ATAAAGAGAG ATTGATGGAG CTTCAGGAAG CTGTTCGATG GACAGAGATG 
ATTCGGGCAT CACGAGAAAA TCCAGCCATG CAGGAAAAAA AAAGGTCAAG CATTTGGCAG 
TTTTTCAGCC GACTTTTCAG CTCCTCAAGT AACACGACTA AGAAGCCTGA ACCACCTGTT 
AATCTGAAGT ACAATGCACC CACGTCTCAT GTTACTCCGT CCGTCAAGAA AAGAAGCAGC 
ACCTTATCTC AGCTCCCTGG GGATAAGTCC AAAGCCTTTG ATTTCCTTAG TGAAGAAACT 
GAAGCTAGTT TAGCCTCACG CAGAGAACAA AAGAGAGAGC AGTATCGTCA GGTAAAAGCA 
CATGTTCAGA AGGAAGACGG TAGAGTGCAG GCTTTTGGCT GGAGTCTGCC TCAGAAGTAC 
AAACAGGTAA CCAATGGTCA AGGTGAAAAT AAGATGAAAA ATTTACCTGT GCCTGTCTAT 
CTCAGACCTC TGGATGAAAA AGATACATCA ATGAAGCTGT GGTGTGCTGT TGGAGTCAAT 
TTATCTGGTG GGAAGACCAG AGATGGTGGT TCTGTTGTTG GAGCAAGTGT ATTTTACAAG 
GATGTTGCTG GTTTGGATAC AGAAGGCAGT AAACAGCGAA GTGCCTCTCA GAGTAGTTTA 
GATAAGTTAG ATCAGGAACT TAAGGAACAG CAGAAGGAGT TAAAAAATCA AGAAGAATTA 
TCCAGTCTAG TTTGGATCTG TACCAGCACT CATTCGGCTA CAAAAGTTCT TATTATTGAT 
GCTGTTCAAC CTGGCAACAT CCTAGACAGT TTCACTGTTT GCAACTCTCA TGTTCTGTGC 
ATTGCAAGTG TGCCAGGTGC ACGAGAAACA GACTACCCTG CAGGAGAAGA TCTTTCAGAA 
TCTGGTCAGG TAGACAAAGC ATCTTTATGT GGAAGTATGA CAAGCAACAG CTCAGCAGAG 
ACAGACAGCC TGTTAGGAGG CATCACAGTG GTTGGTTGTT CTGCAGAAGG TGTGACGGGA 
GCTGCCACTT CCCCTAGTAC AAATGGTGCT TCTCCAGTGA TGGATAAACC ACCAGAAATG 
GAAGCAGAAA ATAGTGAGGT TGATGAAAAT GTTCCAACAG CAGAAGAAGC AACTGAAGCT 
ACAGAAGGGA ATGCGGGGTC AGCTGAAGAC ACAGTGGACA TCTCCCAAAC TGGCGTCTAC 
ACAGAGCATG TCTTTACAGA TCCTTTGGGA GTTCAGATCC CAGAAGACCT CTCCCCAGTG 
TATCAGTCGA GCAATGACTC AGATGCATAT AAAGATCAAA TATCAGTACT GCCAAATGAA 
CAAGACTTGG TGAGAGAAGA AGCCCAGAAA ATGAGTAGTC TTTTACCAAC TATGTGGCTT 
GGAGCTCAAA ATGGCTGTTT GTATGTCCAT TCATCTGTAG CCCAGTGGAG GAAATGTCTC 
CATTCCATTA AACTTAAAGA TTCGATTCTC AGTATTGTAC ACGTGAAGGG AATCGTGTTA 
GTAGCCCTGG CTGACGGCAC CCTTGCAATC TTTCACAGAG GAGTGGATGG GCAGTGGGAT 
TTGTCAAACT ATCACCTCTT AGACCTTGGA CGGCCTCATC ATTCCATCCG TTGCATGACT 
GTGGTACATG ACAAAGTCTG GTGTGGCTAT AGGAACAAAA TCTATGTGGT GCAGCCAAAG 
GCCATGAAAA TAGAGAAATC TTTTGATGCA CATCCCAGGA AGGAGAGCCA AGTGCGACAG 
CTTGCGTGGG TGGGGGATGG CGTGTGGGTC TCCATTCGCT TGGATTCTAC GCTCCGTCTC 
TATCATGCAC ACACTTATCA ACATCTACAG GATGTGGACA TTGAGCCTTA TGTAAGCAAA 
ATGTTAGGTA CTGGAAAACT GGGCTTCTCT TTTGTGAGAA TTACAGCTCT TATGGTGTCT 
TGTAATCGTT TGTGGGTGGG GACAGGAAAT GGTGTCATTA TCTCCATCCC ATTGACAGAA 
ACCGTAATCC TCCACCAGGG ACGTTTACTG GGGCTGAGGG CAAATAAAAC CTCAGGTGTA 
CCAGGAAATC GTCCTGGAAG TGTAATCCGT GTATATGGTG ATGAAAACAG TGATAAAGTG 
ACTCCAGGGA CATTTATACC CTATTGTTCA ATGGCACATG CACAGCTTTG CTTCCATGGG 
CACCGGGATG CTGTGAAATT CTTTGTGGCA GTCCCAGGTC AAGTCATCAG CCCACAAAGT 
AGCAGTAGTG GCACGGATCT GACGGGTGAC AAAGCAGGGC CATCTGCACA GGAGCCTGGT 
AGTCAGACGC CCTTGAAGTC TATGCTTGTC ATCAGTGGAG GAGAGGGCTA CATCGACTTC 
CGAATGGGTG ATGAAGGTGG AGAATCAGAA CTTCTTGGAG AGGATCTTCC ACTTGAACCT 
TCTGTCACCA AAGCAGAAAG GAGTCACTTG ATAGTGTGGC AAGTGATGTA TGGCAATGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_005257774.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257831.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu50774
Clone ID Related Accession (Same CDS sequence)	XM_005257774.4 , XM_005257774.3
Accession Version	XM_005257774.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005257774.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACAAATAAA 
ACCTCAGGTG TACCAGGAAA TCGTCCTGGA AGTGTAATCC GTGTATATGG TGATGAAAAC 
AGTGATAAAG TGACTCCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT 
TGCTTCCATG GGCACCGGGA TGCTGTGAAA TTCTTTGTGG CAGTCCCAGG TCAAGTCATC 
AGCCCACAAA GTAGCAGTAG TGGCACGGAT CTGACGGGTG ACAAAGCAGG GCCATCTGCA 
CAGGAGCCTG GTAGTCAGAC GCCCTTGAAG TCTATGCTTG TCATCAGTGG AGGAGAGGGC 
TACATCGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AACTTCTTGG AGAGGATCTT 
CCACTTGAAC CTTCTGTCAC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG 
TATGGCAATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257831.1
CDS	219..3713
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_005257774.4
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257774.4

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACAAATAAA 
ACCTCAGGTG TACCAGGAAA TCGTCCTGGA AGTGTAATCC GTGTATATGG TGATGAAAAC 
AGTGATAAAG TGACTCCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT 
TGCTTCCATG GGCACCGGGA TGCTGTGAAA TTCTTTGTGG CAGTCCCAGG TCAAGTCATC 
AGCCCACAAA GTAGCAGTAG TGGCACGGAT CTGACGGGTG ACAAAGCAGG GCCATCTGCA 
CAGGAGCCTG GTAGTCAGAC GCCCTTGAAG TCTATGCTTG TCATCAGTGG AGGAGAGGGC 
TACATCGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AACTTCTTGG AGAGGATCTT 
CCACTTGAAC CTTCTGTCAC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG 
TATGGCAATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_005257774.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257831.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.

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CloneID	OHu50774
Clone ID Related Accession (Same CDS sequence)	XM_005257774.4 , XM_005257774.3
Accession Version	XM_005257774.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACAAATAAA 
ACCTCAGGTG TACCAGGAAA TCGTCCTGGA AGTGTAATCC GTGTATATGG TGATGAAAAC 
AGTGATAAAG TGACTCCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT 
TGCTTCCATG GGCACCGGGA TGCTGTGAAA TTCTTTGTGG CAGTCCCAGG TCAAGTCATC 
AGCCCACAAA GTAGCAGTAG TGGCACGGAT CTGACGGGTG ACAAAGCAGG GCCATCTGCA 
CAGGAGCCTG GTAGTCAGAC GCCCTTGAAG TCTATGCTTG TCATCAGTGG AGGAGAGGGC 
TACATCGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AACTTCTTGG AGAGGATCTT 
CCACTTGAAC CTTCTGTCAC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG 
TATGGCAATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257831.1
CDS	225..3719
Translation	MSPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_005257774.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257774.3

ORF Insert Sequence:

ATGAGTCCTG GATGCATGCT GCTGTTTGTG TTTGGCTTTG TTGGCGGGGC GGTGGTCATT 
AATTCTGCTA TCTTAGTATC TCTCTCTGTT TTGCTGCTTG TGCACTTTTC TATTTCTACC 
GGTGTGCCAG CTCTGACGCA GAACCTACCA AGGATACTCA GAAAAGAACG CCCTATATCA 
TTAGGAATTT TCCCATTACC TGCTGGAGAT GGATTGCTTA CACCTGATGC TCAGAAAGGA 
GGAGAGACCC CTGGATCTGA GCAATGGAAA TTTCAGGAAT TAAGTCAACC ACGTTCTCAT 
ACCAGCCTGA AGGATGAGCT TTCTGATGTT AGCCAAGGCG GATCTAAAGC TACCACTCCA 
GCATCAACAG CTAATTCAGA TGTGGCAACA ATTCCTACTG ATACTCCCTT AAAGGAAGAA 
AACGAAGGAT TTGTGAAGGT TACAGATGCG CCAAATAAAT CAGAGATAAG CAAACACATT 
GAAGTACAGG TAGCCCAGGA AACTAGAAAT GTATCTACTG GCTCTGCTGA AAATGAAGAA 
AAGTCAGAAG TTCAAGCAAT CATCGAATCT ACTCCTGAGC TGGATATGGA CAAAGATCTC 
AGTGGATATA AAGGTTCAAG CACTCCCACC AAAGGCATAG AGAACAAAGC TTTTGATCGC 
AATACAGAAT CTCTCTTTGA AGAACTGTCT TCAGCTGGCT CAGGCCTAAT AGGAGATGTG 
GATGAAGGAG CAGATTTACT AGGAATGGGT CGGGAAGTTG AGAATCTTAT ATTAGAAAAT 
ACACAACTGT TGGAAACCAA AAATGCTTTG AACATAGTGA AGAATGATTT GATAGCAAAA 
GTGGATGAAC TGACCTGTGA GAAAGATGTG CTGCAAGGGG AATTGGAGGC TGTGAAGCAA 
GCCAAACTGA AACTAGAGGA AAAGAACAGA GAATTGGAGG AAGAGCTTAG GAAAGCTCGG 
GCAGAAGCTG AAGATGCAAG GCAAAAAGCA AAAGATGACG ATGATAGTGA TATTCCCACA 
GCCCAGAGGA AACGGTTTAC TAGAGTAGAA ATGGCCCGTG TTCTCATGGA GCGAAACCAG 
TATAAAGAGA GATTGATGGA GCTTCAGGAA GCTGTTCGAT GGACAGAGAT GATTCGGGCA 
TCACGAGAAA ATCCAGCCAT GCAGGAAAAA AAAAGGTCAA GCATTTGGCA GTTTTTCAGC 
CGACTTTTCA GCTCCTCAAG TAACACGACT AAGAAGCCTG AACCACCTGT TAATCTGAAG 
TACAATGCAC CCACGTCTCA TGTTACTCCG TCCGTCAAGA AAAGAAGCAG CACCTTATCT 
CAGCTCCCTG GGGATAAGTC CAAAGCCTTT GATTTCCTTA GTGAAGAAAC TGAAGCTAGT 
TTAGCCTCAC GCAGAGAACA AAAGAGAGAG CAGTATCGTC AGGTAAAAGC ACATGTTCAG 
AAGGAAGACG GTAGAGTGCA GGCTTTTGGC TGGAGTCTGC CTCAGAAGTA CAAACAGGTA 
ACCAATGGTC AAGGTGAAAA TAAGATGAAA AATTTACCTG TGCCTGTCTA TCTCAGACCT 
CTGGATGAAA AAGATACATC AATGAAGCTG TGGTGTGCTG TTGGAGTCAA TTTATCTGGT 
GGGAAGACCA GAGATGGTGG TTCTGTTGTT GGAGCAAGTG TATTTTACAA GGATGTTGCT 
GGTTTGGATA CAGAAGGCAG TAAACAGCGA AGTGCCTCTC AGAGTAGTTT AGATAAGTTA 
GATCAGGAAC TTAAGGAACA GCAGAAGGAG TTAAAAAATC AAGAAGAATT ATCCAGTCTA 
GTTTGGATCT GTACCAGCAC TCATTCGGCT ACAAAAGTTC TTATTATTGA TGCTGTTCAA 
CCTGGCAACA TCCTAGACAG TTTCACTGTT TGCAACTCTC ATGTTCTGTG CATTGCAAGT 
GTGCCAGGTG CACGAGAAAC AGACTACCCT GCAGGAGAAG ATCTTTCAGA ATCTGGTCAG 
GTAGACAAAG CATCTTTATG TGGAAGTATG ACAAGCAACA GCTCAGCAGA GACAGACAGC 
CTGTTAGGAG GCATCACAGT GGTTGGTTGT TCTGCAGAAG GTGTGACGGG AGCTGCCACT 
TCCCCTAGTA CAAATGGTGC TTCTCCAGTG ATGGATAAAC CACCAGAAAT GGAAGCAGAA 
AATAGTGAGG TTGATGAAAA TGTTCCAACA GCAGAAGAAG CAACTGAAGC TACAGAAGGG 
AATGCGGGGT CAGCTGAAGA CACAGTGGAC ATCTCCCAAA CTGGCGTCTA CACAGAGCAT 
GTCTTTACAG ATCCTTTGGG AGTTCAGATC CCAGAAGACC TCTCCCCAGT GTATCAGTCG 
AGCAATGACT CAGATGCATA TAAAGATCAA ATATCAGTAC TGCCAAATGA ACAAGACTTG 
GTGAGAGAAG AAGCCCAGAA AATGAGTAGT CTTTTACCAA CTATGTGGCT TGGAGCTCAA 
AATGGCTGTT TGTATGTCCA TTCATCTGTA GCCCAGTGGA GGAAATGTCT CCATTCCATT 
AAACTTAAAG ATTCGATTCT CAGTATTGTA CACGTGAAGG GAATCGTGTT AGTAGCCCTG 
GCTGACGGCA CCCTTGCAAT CTTTCACAGA GGAGTGGATG GGCAGTGGGA TTTGTCAAAC 
TATCACCTCT TAGACCTTGG ACGGCCTCAT CATTCCATCC GTTGCATGAC TGTGGTACAT 
GACAAAGTCT GGTGTGGCTA TAGGAACAAA ATCTATGTGG TGCAGCCAAA GGCCATGAAA 
ATAGAGAAAT CTTTTGATGC ACATCCCAGG AAGGAGAGCC AAGTGCGACA GCTTGCGTGG 
GTGGGGGATG GCGTGTGGGT CTCCATTCGC TTGGATTCTA CGCTCCGTCT CTATCATGCA 
CACACTTATC AACATCTACA GGATGTGGAC ATTGAGCCTT ATGTAAGCAA AATGTTAGGT 
ACTGGAAAAC TGGGCTTCTC TTTTGTGAGA ATTACAGCTC TTATGGTGTC TTGTAATCGT 
TTGTGGGTGG GGACAGGAAA TGGTGTCATT ATCTCCATCC CATTGACAGA AACAAATAAA 
ACCTCAGGTG TACCAGGAAA TCGTCCTGGA AGTGTAATCC GTGTATATGG TGATGAAAAC 
AGTGATAAAG TGACTCCAGG GACATTTATA CCCTATTGTT CAATGGCACA TGCACAGCTT 
TGCTTCCATG GGCACCGGGA TGCTGTGAAA TTCTTTGTGG CAGTCCCAGG TCAAGTCATC 
AGCCCACAAA GTAGCAGTAG TGGCACGGAT CTGACGGGTG ACAAAGCAGG GCCATCTGCA 
CAGGAGCCTG GTAGTCAGAC GCCCTTGAAG TCTATGCTTG TCATCAGTGG AGGAGAGGGC 
TACATCGACT TCCGAATGGG TGATGAAGGT GGAGAATCAG AACTTCTTGG AGAGGATCTT 
CCACTTGAAC CTTCTGTCAC CAAAGCAGAA AGGAGTCACT TGATAGTGTG GCAAGTGATG 
TATGGCAATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAG9 ( XM_005257768.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005257825.1 Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA.

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CloneID	OHu50767
Clone ID Related Accession (Same CDS sequence)	XM_005257768.2
Accession Version	XM_005257768.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	C-Jun-amino-terminal kinase-interacting protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257768.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGTCAGCAAT AGTCCTGAAC CTCAGAAGGC TGTAGAACAG 
GAGGATGAGC TTTCTGATGT TAGCCAAGGC GGATCTAAAG CTACCACTCC AGCATCAACA 
GCTAATTCAG ATGTGGCAAC AATTCCTACT GATACTCCCT TAAAGGAAGA AAACGAAGGA 
TTTGTGAAGG TTACAGATGC GCCAAATAAA TCAGAGATAA GCAAACACAT TGAAGTACAG 
GTAGCCCAGG AAACTAGAAA TGTATCTACT GGCTCTGCTG AAAATGAAGA AAAGTCAGAA 
GTTCAAGCAA TCATCGAATC TACTCCTGAG CTGGATATGG ACAAAGATCT CAGTGGATAT 
AAAGGTTCAA GCACTCCCAC CAAAGGCATA GAGAACAAAG CTTTTGATCG CAATACAGAA 
TCTCTCTTTG AAGAACTGTC TTCAGCTGGC TCAGGCCTAA TAGGAGATGT GGATGAAGGA 
GCAGATTTAC TAGGAATGGG TCGGGAAGTT GAGAATCTTA TATTAGAAAA TACACAACTG 
TTGGAAACCA AAAATGCTTT GAACATAGTG AAGAATGATT TGATAGCAAA AGTGGATGAA 
CTGACCTGTG AGAAAGATGT GCTGCAAGGG GAATTGGAGG CTGTGAAGCA AGCCAAACTG 
AAACTAGAGG AAAAGAACAG AGAATTGGAG GAAGAGCTTA GGAAAGCTCG GGCAGAAGCT 
GAAGATGCAA GGCAAAAAGC AAAAGATGAC GATGATAGTG ATATTCCCAC AGCCCAGAGG 
AAACGGTTTA CTAGAGTAGA AATGGCCCGT GTTCTCATGG AGCGAAACCA GTATAAAGAG 
AGATTGATGG AGCTTCAGGA AGCTGTTCGA TGGACAGAGA TGATTCGGGC ATCACGAGAA 
AATCCAGCCA TGCAGGAAAA AAAAAGGTCA AGCATTTGGC AGTTTTTCAG CCGACTTTTC 
AGCTCCTCAA GTAACACGAC TAAGAAGCCT GAACCACCTG TTAATCTGAA GTACAATGCA 
CCCACGTCTC ATGTTACTCC GTCCGTCAAG AAAAGAAGCA GCACCTTATC TCAGCTCCCT 
GGGGATAAGT CCAAAGCCTT TGATTTCCTT AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA 
CGCAGAGAAC AAAAGAGAGA GCAGTATCGT CAGGTAAAAG CACATGTTCA GAAGGAAGAC 
GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG CCTCAGAAGT ACAAACAGGT AACCAATGGT 
CAAGGTGAAA ATAAGATGAA AAATTTACCT GTGCCTGTCT ATCTCAGACC TCTGGATGAA 
AAAGATACAT CAATGAAGCT GTGGTGTGCT GTTGGAGTCA ATTTATCTGG TGGGAAGACC 
AGAGATGGTG GTTCTGTTGT TGGAGCAAGT GTATTTTACA AGGATGTTGC TGGTTTGGAT 
ACAGAAGGCA GTAAACAGCG AAGTGCCTCT CAGAGTAGTT TAGATAAGTT AGATCAGGAA 
CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT CAAGAAGAAT TATCCAGTCT AGTTTGGATC 
TGTACCAGCA CTCATTCGGC TACAAAAGTT CTTATTATTG ATGCTGTTCA ACCTGGCAAC 
ATCCTAGACA GTTTCACTGT TTGCAACTCT CATGTTCTGT GCATTGCAAG TGTGCCAGGT 
GCACGAGAAA CAGACTACCC TGCAGGAGAA GATCTTTCAG AATCTGGTCA GGTAGACAAA 
GCATCTTTAT GTGGAAGTAT GACAAGCAAC AGCTCAGCAG AGACAGACAG CCTGTTAGGA 
GGCATCACAG TGGTTGGTTG TTCTGCAGAA GGTGTGACGG GAGCTGCCAC TTCCCCTAGT 
ACAAATGGTG CTTCTCCAGT GATGGATAAA CCACCAGAAA TGGAAGCAGA AAATAGTGAG 
GTTGATGAAA ATGTTCCAAC AGCAGAAGAA GCAACTGAAG CTACAGAAGG GAATGCGGGG 
TCAGCTGAAG ACACAGTGGA CATCTCCCAA ACTGGCGTCT ACACAGAGCA TGTCTTTACA 
GATCCTTTGG GAGTTCAGAT CCCAGAAGAC CTCTCCCCAG TGTATCAGTC GAGCAATGAC 
TCAGATGCAT ATAAAGATCA AATATCAGTA CTGCCAAATG AACAAGACTT GGTGAGAGAA 
GAAGCCCAGA AAATGAGTAG TCTTTTACCA ACTATGTGGC TTGGAGCTCA AAATGGCTGT 
TTGTATGTCC ATTCATCTGT AGCCCAGTGG AGGAAATGTC TCCATTCCAT TAAACTTAAA 
GATTCGATTC TCAGTATTGT ACACGTGAAG GGAATCGTGT TAGTAGCCCT GGCTGACGGC 
ACCCTTGCAA TCTTTCACAG AGGAGTGGAT GGGCAGTGGG ATTTGTCAAA CTATCACCTC 
TTAGACCTTG GACGGCCTCA TCATTCCATC CGTTGCATGA CTGTGGTACA TGACAAAGTC 
TGGTGTGGCT ATAGGAACAA AATCTATGTG GTGCAGCCAA AGGCCATGAA AATAGAGAAA 
TCTTTTGATG CACATCCCAG GAAGGAGAGC CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT 
GGCGTGTGGG TCTCCATTCG CTTGGATTCT ACGCTCCGTC TCTATCATGC ACACACTTAT 
CAACATCTAC AGGATGTGGA CATTGAGCCT TATGTAAGCA AAATGTTAGG TACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATTACAGCT CTTATGGTGT CTTGTAATCG TTTGTGGGTG 
GGGACAGGAA ATGGTGTCAT TATCTCCATC CCATTGACAG AAACCGTAAT CCTCCACCAG 
GGACGTTTAC TGGGGCTGAG GGCAAATAAA ACCTCAGGTG TACCAGGAAA TCGTCCTGGA 
AGTGTAATCC GTGTATATGG TGATGAAAAC AGTGATAAAG TGACTCCAGG GACATTTATA 
CCCTATTGTT CAATGGCACA TGCACAGCTT TGCTTCCATG GGCACCGGGA TGCTGTGAAA 
TTCTTTGTGG CAGTCCCAGG TCAAGTCATC AGCCCACAAA GTAGCAGTAG TGGCACGGAT 
CTGACGGGTG ACAAAGCAGG GCCATCTGCA CAGGAGCCTG GTAGTCAGAC GCCCTTGAAG 
TCTATGCTTG TCATCAGTGG AGGAGAGGGC TACATCGACT TCCGAATGGG TGATGAAGGT 
GGAGAATCAG AACTTCTTGG AGAGGATCTT CCACTTGAAC CTTCTGTCAC CAAAGCAGAA 
AGGAGTCACT TGATAGTGTG GCAAGTGATG TATGGCAATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005257825.1
CDS	210..4214
Translation	MELEDGVVYQEEPGGSGAVMSERVSGLAGSIYREFERLIGRYDEEVVKELMPLVVAVLENLDSVFAQDQEHQVELELLRDDNEQLITQYEREKALRKHAEEKFIEFEDSQEQEKKDLQTRVESLESQTRQLELKAKNYADQISRLEEREAELKKEYNALHQRHTEMIHNYMEHLERTKLHQLSGSDQLESTAHSRIRKERPISLGIFPLPAGDGLLTPDAQKGGETPGSEQWKFQELSQPRSHTSLKVSNSPEPQKAVEQEDELSDVSQGGSKATTPASTANSDVATIPTDTPLKEENEGFVKVTDAPNKSEISKHIEVQVAQETRNVSTGSAENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPTKGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNIVKNDLIAKVDELTCEKDVLQGELEAVKQAKLKLEEKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNTTKKPEPPVNLKYNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFGWSLPQKYKQVTNGQGENKMKNLPVPVYLRPLDEKDTSMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDVAGLDTEGSKQRSASQSSLDKLDQELKEQQKELKNQEELSSLVWICTSTHSATKVLIIDAVQPGNILDSFTVCNSHVLCIASVPGARETDYPAGEDLSESGQVDKASLCGSMTSNSSAETDSLLGGITVVGCSAEGVTGAATSPSTNGASPVMDKPPEMEAENSEVDENVPTAEEATEATEGNAGSAEDTVDISQTGVYTEHVFTDPLGVQIPEDLSPVYQSSNDSDAYKDQISVLPNEQDLVREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSIKLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNKIYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLGTGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETVILHQGRLLGLRANKTSGVPGNRPGSVIRVYGDENSDKVTPGTFIPYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSSGTDLTGDKAGPSAQEPGSQTPLKSMLVISGGEGYIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMYGNE

Target ORF information:

RefSeq Version	XM_005257768.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sperm associated antigen 9 (SPAG9), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257768.2

ORF Insert Sequence:

ATGGAGCTGG AGGACGGTGT GGTGTATCAG GAGGAGCCCG GCGGCTCCGG GGCCGTGATG 
TCGGAGCGGG TGTCCGGCCT GGCCGGCTCC ATCTACCGCG AGTTCGAGCG GCTTATCGGG 
CGCTATGACG AGGAGGTGGT CAAAGAGCTG ATGCCGCTGG TGGTGGCTGT GCTGGAGAAC 
CTGGACTCGG TGTTCGCGCA GGACCAGGAG CACCAGGTGG AGCTGGAGCT GCTGCGGGAC 
GACAACGAGC AGCTCATCAC CCAGTACGAG CGGGAGAAGG CGCTGCGCAA GCACGCTGAG 
GAGAAATTCA TTGAATTTGA AGACTCTCAA GAACAGGAAA AAAAGGACTT ACAGACCCGA 
GTGGAATCTT TAGAATCTCA AACAAGACAA CTTGAGCTGA AAGCGAAAAA CTATGCTGAC 
CAGATTAGCA GACTTGAAGA AAGAGAAGCA GAACTGAAGA AGGAATATAA TGCATTACAT 
CAAAGACACA CTGAGATGAT CCATAATTAT ATGGAACATT TAGAAAGAAC AAAACTTCAT 
CAGCTCTCAG GGAGTGATCA ACTAGAATCC ACAGCTCATA GTAGAATTAG AAAAGAACGC 
CCTATATCAT TAGGAATTTT CCCATTACCT GCTGGAGATG GATTGCTTAC ACCTGATGCT 
CAGAAAGGAG GAGAGACCCC TGGATCTGAG CAATGGAAAT TTCAGGAATT AAGTCAACCA 
CGTTCTCATA CCAGCCTGAA GGTCAGCAAT AGTCCTGAAC CTCAGAAGGC TGTAGAACAG 
GAGGATGAGC TTTCTGATGT TAGCCAAGGC GGATCTAAAG CTACCACTCC AGCATCAACA 
GCTAATTCAG ATGTGGCAAC AATTCCTACT GATACTCCCT TAAAGGAAGA AAACGAAGGA 
TTTGTGAAGG TTACAGATGC GCCAAATAAA TCAGAGATAA GCAAACACAT TGAAGTACAG 
GTAGCCCAGG AAACTAGAAA TGTATCTACT GGCTCTGCTG AAAATGAAGA AAAGTCAGAA 
GTTCAAGCAA TCATCGAATC TACTCCTGAG CTGGATATGG ACAAAGATCT CAGTGGATAT 
AAAGGTTCAA GCACTCCCAC CAAAGGCATA GAGAACAAAG CTTTTGATCG CAATACAGAA 
TCTCTCTTTG AAGAACTGTC TTCAGCTGGC TCAGGCCTAA TAGGAGATGT GGATGAAGGA 
GCAGATTTAC TAGGAATGGG TCGGGAAGTT GAGAATCTTA TATTAGAAAA TACACAACTG 
TTGGAAACCA AAAATGCTTT GAACATAGTG AAGAATGATT TGATAGCAAA AGTGGATGAA 
CTGACCTGTG AGAAAGATGT GCTGCAAGGG GAATTGGAGG CTGTGAAGCA AGCCAAACTG 
AAACTAGAGG AAAAGAACAG AGAATTGGAG GAAGAGCTTA GGAAAGCTCG GGCAGAAGCT 
GAAGATGCAA GGCAAAAAGC AAAAGATGAC GATGATAGTG ATATTCCCAC AGCCCAGAGG 
AAACGGTTTA CTAGAGTAGA AATGGCCCGT GTTCTCATGG AGCGAAACCA GTATAAAGAG 
AGATTGATGG AGCTTCAGGA AGCTGTTCGA TGGACAGAGA TGATTCGGGC ATCACGAGAA 
AATCCAGCCA TGCAGGAAAA AAAAAGGTCA AGCATTTGGC AGTTTTTCAG CCGACTTTTC 
AGCTCCTCAA GTAACACGAC TAAGAAGCCT GAACCACCTG TTAATCTGAA GTACAATGCA 
CCCACGTCTC ATGTTACTCC GTCCGTCAAG AAAAGAAGCA GCACCTTATC TCAGCTCCCT 
GGGGATAAGT CCAAAGCCTT TGATTTCCTT AGTGAAGAAA CTGAAGCTAG TTTAGCCTCA 
CGCAGAGAAC AAAAGAGAGA GCAGTATCGT CAGGTAAAAG CACATGTTCA GAAGGAAGAC 
GGTAGAGTGC AGGCTTTTGG CTGGAGTCTG CCTCAGAAGT ACAAACAGGT AACCAATGGT 
CAAGGTGAAA ATAAGATGAA AAATTTACCT GTGCCTGTCT ATCTCAGACC TCTGGATGAA 
AAAGATACAT CAATGAAGCT GTGGTGTGCT GTTGGAGTCA ATTTATCTGG TGGGAAGACC 
AGAGATGGTG GTTCTGTTGT TGGAGCAAGT GTATTTTACA AGGATGTTGC TGGTTTGGAT 
ACAGAAGGCA GTAAACAGCG AAGTGCCTCT CAGAGTAGTT TAGATAAGTT AGATCAGGAA 
CTTAAGGAAC AGCAGAAGGA GTTAAAAAAT CAAGAAGAAT TATCCAGTCT AGTTTGGATC 
TGTACCAGCA CTCATTCGGC TACAAAAGTT CTTATTATTG ATGCTGTTCA ACCTGGCAAC 
ATCCTAGACA GTTTCACTGT TTGCAACTCT CATGTTCTGT GCATTGCAAG TGTGCCAGGT 
GCACGAGAAA CAGACTACCC TGCAGGAGAA GATCTTTCAG AATCTGGTCA GGTAGACAAA 
GCATCTTTAT GTGGAAGTAT GACAAGCAAC AGCTCAGCAG AGACAGACAG CCTGTTAGGA 
GGCATCACAG TGGTTGGTTG TTCTGCAGAA GGTGTGACGG GAGCTGCCAC TTCCCCTAGT 
ACAAATGGTG CTTCTCCAGT GATGGATAAA CCACCAGAAA TGGAAGCAGA AAATAGTGAG 
GTTGATGAAA ATGTTCCAAC AGCAGAAGAA GCAACTGAAG CTACAGAAGG GAATGCGGGG 
TCAGCTGAAG ACACAGTGGA CATCTCCCAA ACTGGCGTCT ACACAGAGCA TGTCTTTACA 
GATCCTTTGG GAGTTCAGAT CCCAGAAGAC CTCTCCCCAG TGTATCAGTC GAGCAATGAC 
TCAGATGCAT ATAAAGATCA AATATCAGTA CTGCCAAATG AACAAGACTT GGTGAGAGAA 
GAAGCCCAGA AAATGAGTAG TCTTTTACCA ACTATGTGGC TTGGAGCTCA AAATGGCTGT 
TTGTATGTCC ATTCATCTGT AGCCCAGTGG AGGAAATGTC TCCATTCCAT TAAACTTAAA 
GATTCGATTC TCAGTATTGT ACACGTGAAG GGAATCGTGT TAGTAGCCCT GGCTGACGGC 
ACCCTTGCAA TCTTTCACAG AGGAGTGGAT GGGCAGTGGG ATTTGTCAAA CTATCACCTC 
TTAGACCTTG GACGGCCTCA TCATTCCATC CGTTGCATGA CTGTGGTACA TGACAAAGTC 
TGGTGTGGCT ATAGGAACAA AATCTATGTG GTGCAGCCAA AGGCCATGAA AATAGAGAAA 
TCTTTTGATG CACATCCCAG GAAGGAGAGC CAAGTGCGAC AGCTTGCGTG GGTGGGGGAT 
GGCGTGTGGG TCTCCATTCG CTTGGATTCT ACGCTCCGTC TCTATCATGC ACACACTTAT 
CAACATCTAC AGGATGTGGA CATTGAGCCT TATGTAAGCA AAATGTTAGG TACTGGAAAA 
CTGGGCTTCT CTTTTGTGAG AATTACAGCT CTTATGGTGT CTTGTAATCG TTTGTGGGTG 
GGGACAGGAA ATGGTGTCAT TATCTCCATC CCATTGACAG AAACCGTAAT CCTCCACCAG 
GGACGTTTAC TGGGGCTGAG GGCAAATAAA ACCTCAGGTG TACCAGGAAA TCGTCCTGGA 
AGTGTAATCC GTGTATATGG TGATGAAAAC AGTGATAAAG TGACTCCAGG GACATTTATA 
CCCTATTGTT CAATGGCACA TGCACAGCTT TGCTTCCATG GGCACCGGGA TGCTGTGAAA 
TTCTTTGTGG CAGTCCCAGG TCAAGTCATC AGCCCACAAA GTAGCAGTAG TGGCACGGAT 
CTGACGGGTG ACAAAGCAGG GCCATCTGCA CAGGAGCCTG GTAGTCAGAC GCCCTTGAAG 
TCTATGCTTG TCATCAGTGG AGGAGAGGGC TACATCGACT TCCGAATGGG TGATGAAGGT 
GGAGAATCAG AACTTCTTGG AGAGGATCTT CCACTTGAAC CTTCTGTCAC CAAAGCAGAA 
AGGAGTCACT TGATAGTGTG GCAAGTGATG TATGGCAATG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes.
Nature communications8(1)1580(2017 Nov)
Willett R,Martina JA,Zewe JP,Wills R,Hammond GRV,Puertollano R

Aberrant expression of JNK-associated leucine-zipper protein, JLP, promotes accelerated growth of ovarian cancer.
Oncotarget7(45)72845-72859(2016 Nov)
Ha JH,Yan M,Gomathinayagam R,Jayaraman M,Husain S,Liu J,Mukherjee P,Reddy EP,Song YS,Dhanasekaran DN

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

SPAG9 promotes endometrial carcinoma cell invasion through regulation of genes related to the epithelial-mesenchymal transition.
European journal of gynaecological oncology37(3)312-9(2016)
Zhang L,Yan L,Cao M,Zhang H,Li C,Bai Y,Yu P,Li M,Zhao X

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SPAG9 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム