UBE2M cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2M
Entrez Gene ID	9040
Full Name	ubiquitin conjugating enzyme E2 M
Synonyms	UBC-RS2,UBC12,hUbc12
General protein information	Preferred Names ubiquitin conjugating enzyme E2 M Names NEDD8-conjugating enzyme Ubc12 NEDD8 carrier protein NEDD8 protein ligase ubiquitin carrier protein M ubiquitin conjugating enzyme E2M ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast) ubiquitin-protein ligase M yeast UBC12 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.43
Summary	The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008].
Disorder MIM:	603173

mRNA and Protein(s)

mRNA	Protein	Name
NM_003969.4	NP_003960.1	NEDD8-conjugating enzyme Ubc12
NM_003969.3	NP_003960.1	NEDD8-conjugating enzyme Ubc12

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2M	XP_001146573.2
	UBE2M	XP_001099932.1
Bos taurus (cattle)	UBE2M	NP_001076972.1
Schizosaccharomyces pombe (fission yeast)	ubc12	NP_588256.1
Mus musculus (house mouse)	Ube2m	NP_663553.1
Magnaporthe oryzae (rice blast fungus)	MGG_01807	XP_003714818.1
Neurospora crassa	NCU03610	XP_961337.1
Oryza sativa (rice)	Os09g0321900	NP_001062859.1
Homo sapiens (human)	UBE2M	NP_003960.1
Canis lupus familiaris (dog)	UBE2M	XP_541337.3
Drosophila melanogaster (fruit fly)	Ubc12	NP_001261568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005916	XP_315947.2
Saccharomyces cerevisiae (baker's yeast)	UBC12	NP_013409.1
Arabidopsis thaliana (thale cress)	RCE1	NP_001154289.1
Arabidopsis thaliana (thale cress)	AT2G18600	NP_565440.1
Rattus norvegicus (Norway rat)	Ube2m	NP_001101941.1
Danio rerio (zebrafish)	wu:fb58e05	XP_001920670.3
Kluyveromyces lactis	KLLA0C17248g	XP_452971.1
Oryza sativa (rice)	Os08g0374100	NP_001061664.1
Eremothecium gossypii	AGOS_ADL035C	NP_984062.1
Xenopus tropicalis (tropical clawed frog)	ube2m	NP_988956.1

Gene Ontology
Bibliography

Related articles in PubMed

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.
Zhou H, Lu J, Liu L, Bernard D, Yang CY, Fernandez-Salas E, Chinnaswamy K, Layton S, Stuckey J, Yu Q, Zhou W, Pan Z, Sun Y, Wang S
Nature communications8(1)1150(2017 Oct)

NEDDylation antagonizes ubiquitination of proliferating cell nuclear antigen and regulates the recruitment of polymerase ? in response to oxidative DNA damage.
Guan J, Yu S, Zheng X
Protein & cell(2017 Aug)

Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase.
Scott DC, Hammill JT, Min J, Rhee DY, Connelly M, Sviderskiy VO, Bhasin D, Chen Y, Ong SS, Chai SC, Goktug AN, Huang G, Monda JK, Low J, Kim HS, Paulo JA, Cannon JR, Shelat AA, Chen T, Kelsall IR, Alpi AF, Pagala V, Wang X, Peng J, Singh B, Harper JW, Schulman BA, Guy RK
Nature chemical biology13(8)850-857(2017 Aug)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

GlyRS preferentially binds and protects Ubc12.
Title: Neddylation requires glycyl-tRNA synthetase to protect activated E2.

Polymorphisms in the UBE2M gene are associated with cetuximab efficacy in colorectal cancer.
Title: Polymorphisms in Genes Involved in EGFR Turnover Are Predictive for Cetuximab Efficacy in Colorectal Cancer.

E1 (NAE1 and UBA3) and E2 (UBC12) enzymes, as well as global NEDD8 conjugation, were upregulated in over 2/3 of human intrahepatic cholangiocarcinoma
Title: Neddylation pathway is up-regulated in human intrahepatic cholangiocarcinoma and serves as a potential therapeutic target.

UBE2M is required to maintain genome integrity by activating multiple Cullin ligases throughout the cell cycle.
Title: Inactivating UBE2M impacts the DNA damage response and genome integrity involving multiple cullin ligases.

Distinct preferences of UBC12 and UBE2F peptides for inhibiting different DCNLs, including the oncogenic DCNL1.
Title: Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.

The following UBE2M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2M cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10563	NM_003969.4 Latest version!	Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu10563	NM_003969.3	Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2M ( NM_003969.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003960.1 Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.

Price & Availability↑

CloneID	OHu10563
Clone ID Related Accession (Same CDS sequence)	NM_003969.4 , NM_003969.3
Accession Version	NM_003969.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	NEDD8-conjugating enzyme Ubc12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016630.6 and BC058924.1. On May 17, 2019 this sequence version replaced NM_003969.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.204094.1, SRR1163658.422502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000253023.8/ ENSP00000253023.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC GGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTGCGGA TCCAGAAGGA CATAAACGAG 
CTGAACCTGC CCAAGACGTG TGATATCAGC TTCTCAGATC CAGACGACCT CCTCAACTTC 
AAGCTGGTCA TCTGTCCTGA TGAGGGCTTC TACAAGAGTG GGAAGTTTGT GTTCAGTTTT 
AAGGTGGGCC AGGGTTACCC GCATGATCCC CCCAAGGTGA AGTGTGAGAC AATGGTCTAT 
CACCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAGGACC CACTGAACAA GGAGGCCGCA GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAGCAGAACG TGCAGCGCTC CATGCGGGGT GGCTACATCG GCTCCACCTA CTTTGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003960.1
CDS	231..782
Translation	MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	NM_003969.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003969.4

ORF Insert Sequence:

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC GGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTGCGGA TCCAGAAGGA CATAAACGAG 
CTGAACCTGC CCAAGACGTG TGATATCAGC TTCTCAGATC CAGACGACCT CCTCAACTTC 
AAGCTGGTCA TCTGTCCTGA TGAGGGCTTC TACAAGAGTG GGAAGTTTGT GTTCAGTTTT 
AAGGTGGGCC AGGGTTACCC GCATGATCCC CCCAAGGTGA AGTGTGAGAC AATGGTCTAT 
CACCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAGGACC CACTGAACAA GGAGGCCGCA GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAGCAGAACG TGCAGCGCTC CATGCGGGGT GGCTACATCG GCTCCACCTA CTTTGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UBE2M ( NM_003969.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003960.1 Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.

Price & Availability↑

CloneID	OHu10563
Clone ID Related Accession (Same CDS sequence)	NM_003969.4 , NM_003969.3
Accession Version	NM_003969.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	NEDD8-conjugating enzyme Ubc12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM798750.1, AC016630.6 and BC058924.1. On Jul 3, 2007 this sequence version replaced NM_003969.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.262687.1, SRR1163657.13181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC GGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTGCGGA TCCAGAAGGA CATAAACGAG 
CTGAACCTGC CCAAGACGTG TGATATCAGC TTCTCAGATC CAGACGACCT CCTCAACTTC 
AAGCTGGTCA TCTGTCCTGA TGAGGGCTTC TACAAGAGTG GGAAGTTTGT GTTCAGTTTT 
AAGGTGGGCC AGGGTTACCC GCATGATCCC CCCAAGGTGA AGTGTGAGAC AATGGTCTAT 
CACCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAGGACC CACTGAACAA GGAGGCCGCA GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAGCAGAACG TGCAGCGCTC CATGCGGGGT GGCTACATCG GCTCCACCTA CTTTGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003960.1
CDS	596..1147
Translation	MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	NM_003969.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003969.3

ORF Insert Sequence:

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC GGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCGGCG CAGCTGCGGA TCCAGAAGGA CATAAACGAG 
CTGAACCTGC CCAAGACGTG TGATATCAGC TTCTCAGATC CAGACGACCT CCTCAACTTC 
AAGCTGGTCA TCTGTCCTGA TGAGGGCTTC TACAAGAGTG GGAAGTTTGT GTTCAGTTTT 
AAGGTGGGCC AGGGTTACCC GCATGATCCC CCCAAGGTGA AGTGTGAGAC AATGGTCTAT 
CACCCCAACA TTGACCTCGA GGGCAACGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAGGACC CACTGAACAA GGAGGCCGCA GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAGCAGAACG TGCAGCGCTC CATGCGGGGT GGCTACATCG GCTCCACCTA CTTTGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.
Nature communications8(1)1150(2017 Oct)
Zhou H,Lu J,Liu L,Bernard D,Yang CY,Fernandez-Salas E,Chinnaswamy K,Layton S,Stuckey J,Yu Q,Zhou W,Pan Z,Sun Y,Wang S

NEDDylation antagonizes ubiquitination of proliferating cell nuclear antigen and regulates the recruitment of polymerase ? in response to oxidative DNA damage.
Protein & cell(2017 Aug)
Guan J,Yu S,Zheng X

Blocking an N-terminal acetylation-dependent protein interaction inhibits an E3 ligase.
Nature chemical biology13(8)850-857(2017 Aug)
Scott DC,Hammill JT,Min J,Rhee DY,Connelly M,Sviderskiy VO,Bhasin D,Chen Y,Ong SS,Chai SC,Goktug AN,Huang G,Monda JK,Low J,Kim HS,Paulo JA,Cannon JR,Shelat AA,Chen T,Kelsall IR,Alpi AF,Pagala V,Wang X,Peng J,Singh B,Harper JW,Schulman BA,Guy RK

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

UBE2M cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報